CN103540548A - Compound fungicide for low-temperature compost, and preparation method and application thereof - Google Patents
Compound fungicide for low-temperature compost, and preparation method and application thereof Download PDFInfo
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- CN103540548A CN103540548A CN201310488121.0A CN201310488121A CN103540548A CN 103540548 A CN103540548 A CN 103540548A CN 201310488121 A CN201310488121 A CN 201310488121A CN 103540548 A CN103540548 A CN 103540548A
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- 239000002361 compost Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims description 14
- 230000000855 fungicidal effect Effects 0.000 title abstract description 5
- 239000000417 fungicide Substances 0.000 title abstract description 5
- 150000001875 compounds Chemical class 0.000 title abstract 4
- 230000001580 bacterial effect Effects 0.000 claims abstract description 24
- 241000233866 Fungi Species 0.000 claims description 37
- 239000003795 chemical substances by application Substances 0.000 claims description 37
- 239000002131 composite material Substances 0.000 claims description 37
- 241000190932 Rhodopseudomonas Species 0.000 claims description 17
- 230000004913 activation Effects 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 235000019319 peptone Nutrition 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 235000013379 molasses Nutrition 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 7
- 241000894006 Bacteria Species 0.000 abstract description 4
- 241000589516 Pseudomonas Species 0.000 abstract 2
- 230000000737 periodic effect Effects 0.000 abstract 1
- 238000009264 composting Methods 0.000 description 10
- 239000000463 material Substances 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
- 230000005526 G1 to G0 transition Effects 0.000 description 2
- 241000589774 Pseudomonas sp. Species 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention relates to a compound fungicide for low-temperature compost, which is composed of the following bacterial strains: 1.0*10<10>-1.0*10<12> cfu/mL of pseudomonas B6-15, and 1.0*10<10>-1.0*10<12> cfu/mL of pseudomonas B5-16, wherein the total bacteria count is 2.0*10<10>-2.0*10<12> cfu/mL. When the compound fungicide disclosed by the invention is used at low temperature, a periodic function can be played in the temperature increasing process of compost; the time required in the temperature increasing stage is obviously shortened; the temperature of the compost is increased; the compound fungicide disclosed by the invention has a certain practical significance for solving the temperature increasing difficulty of the compost at low temperature in winter in north china.
Description
Technical field
The present invention relates to a kind of composite fungus agent for low temperature compost and its preparation method and application, belong to microbial preparation field.
Background technology
China's agricultural wastes of a great variety, wherein, feces of livestock and poultry year generation be about 18.6 * 10
8t, wherein cow dung 10.7 * 10
8t, pig manure 2.7 * 10
8t, sheep excrement 3.4 * 10
8t, poultry manure 1.8 * 10
8t, and along with the surge with the size of population that develops rapidly of industrial and agricultural production, these wastes are with the speed increase in year 5%～10%.Composting technology, as a kind of product that keeps good environment effect, has the sustainability of biological treatment and the features such as recycle of waste resource, by many countries and regions, is accepted, and becomes one of effective ways of processing organic solid waste.
Microorganism adds as a kind of additive in composting process, i.e. microbial inoculant.Commercially available common microbial inoculant is generally mesophilic bacteria agent or high temperature microbial inoculum.But under low temperature environment, the large heavy discount of the effect of these microbial inoculants in composting process, causes time temperature rise period long, has affected compost hazard-free process and fermentation period.Therefore, filter out that effectively to shorten the composite microbial inoculant fungus strain of heating-up time significant for North China's winter low temperature compost.
Summary of the invention
The object of this invention is to provide a kind of composite fungus agent of being rapidly heated of in earlier stage helping for low temperature compost.
In order to realize object of the present invention, the present invention adopts following technical scheme.
For a composite fungus agent for low temperature compost, by following bacterial strain, formed:
Rhodopseudomonas B6-15 1.0 * 10
10～1.0 * 10
12cfu/mL;
Rhodopseudomonas B5-16 1.0 * 10
10～1.0 * 10
12cfu/mL;
Total count is 2.0 * 10
10-2.0 * 10
12cfu/mL.
As the preferred embodiment of the present invention, the described composite fungus agent for low temperature compost, is comprised of following bacterial strain:
Rhodopseudomonas B6-15 2.6 * 10
10～20.0 * 10
10cfu/mL;
Rhodopseudomonas B5-16 1.8 * 10
10～15.0 * 10
10cfu/mL.
Rhodopseudomonas B6-15 of the present invention (Pseudomonas sp.), Rhodopseudomonas B5-16(Pseudomonas sp.) all can buy and obtain by Beijing Voto Sky & Land Biotech Co., Ltd..
The present invention also provides the preparation method of above-mentioned composite fungus agent, comprises the steps:
1) slant culture: by B6-15, be inoculated in respectively on solid medium under B5-16 original strain aseptic condition, cultivate 1-2 days under 15-25 ℃ of condition, activation bacterial strain;
2) first order seed cultivation: by step 1) under the bacterial classification aseptic condition of activation, be inoculated in respectively NR liquid nutrient medium, 15-25 ℃ of shaking table cultivated 1-2 days, makes primary seed solution;
3) mixing fermentation culture: by step 2) primary seed solution B6-15, B5-16 are inoculated in fermentor tank, and mixed culture 3-5 days under 20 ℃ of conditions, obtains composite fungus agent.
Wherein, in step 1), described solid culture based formulas is: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, agar 15g/L, deionized water 1000ml, sterilizing.
In step 2) in, described NR liquid culture based formulas is: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, deionized water 1000ml, sterilizing.
In step 2) in, described shaking table culture condition is: air flow 1:1-1.2,150-200r/min.
In step 3), described fermentative medium formula is: molasses 15%, corn steep liquor 2%, MgSO
40.05%, K
2hPO
40.15%, NaCl0.1%, glucose 1.5%.
In step 3), described inoculum size is the 1-10% of fermention medium volume.
The present invention also provides the application of composite fungus agent in low temperature compost.Be specially composite fungus agent in the 3-6 ‰ access composting material with composting material dry weight, 2-4 ℃ is carried out compost 15-48h.
Complex micro organism fungicide of the present invention has fast growth under cold condition, and symbiosis, the collaborative feature that promotes and do not produce antagonistic action, cultivate 20h and reach stationary phase, OD
600=2.9; In the process of going down to posterity, bacterial strain quantity stability remains on higher level, and pH is more stable in growth, between 7.5-8.
Under cold condition, use composite fungus agent of the present invention, can in compost temperature-rise period, play interim effect, significantly shorten compost temperature rise period required time, for solving under domestic northern area winter low temperature envrionment conditions the compost difficult present situation that heats up, there is certain realistic meaning.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
A composite fungus agent for low temperature compost, consists of:
Rhodopseudomonas B6-15 2.6 * 10
10cfu/mL;
Rhodopseudomonas B5-16 1.8 * 10
10cfu/mL;
The total content value range of two kinds of bacterial strains is 3.0 * 10
10-6.0 * 10
10cfu/mL.
The preparation method of above-mentioned composite fungus agent, comprises the steps:
1) slant culture: by B6-15, be inoculated in respectively on solid medium under B5-16 original strain aseptic condition, cultivate 1 day under 20 ℃ of conditions, activation bacterial strain;
2) first order seed cultivation: by step 1) under the bacterial classification aseptic condition of activation, be inoculated in respectively NR liquid nutrient medium, under 20 ℃ of conditions, air flow 1:1.2,160r/min shaking table is cultivated 1 day, makes primary seed solution;
3) mixing fermentation culture: by step 2) primary seed solution B6-15, B5-16 is inoculated in fermentor tank according to 5% of fermention medium volume, cultivates 3 days, obtains composite fungus agent.
Wherein, in step 1), described solid culture based formulas is: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, agar 15g/L, deionized water 1000ml, sterilizing.
In step 3), described fermentative medium formula is: molasses 15%, corn steep liquor 2%, MgSO
40.05%, K
2hPO
40.15%, NaCl0.1%, glucose 1.5%.
Embodiment 2
For a composite fungus agent for low temperature compost, form:
Rhodopseudomonas B6-15 5.0 * 10
10cfu/mL;
Rhodopseudomonas B5-16 4.5 * 10
10cfu/mL;
The total content value range of two kinds of bacterial strains is 9.0 * 10
10-1.0 * 10
12cfu/mL.
The preparation method of above-mentioned composite fungus agent, comprises the steps:
1) slant culture: by B6-15, be inoculated in respectively on solid medium under B5-16 original strain aseptic condition, cultivate 1 day under 20 ℃ of conditions, activation bacterial strain;
2) first order seed cultivation: by step 1) under the bacterial classification aseptic condition of activation, be inoculated in respectively NR liquid nutrient medium, under 20 ℃ of conditions, air flow 1:1.2,160r/min shaking table is cultivated 1 day, makes primary seed solution;
3) mixing fermentation culture: by step 2) primary seed solution B6-15, B5-16 is inoculated in fermentor tank according to 5% of fermention medium volume, cultivates 3 days, obtains composite fungus agent.
Wherein, in step 1), described solid culture based formulas is: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, agar 15g/L, deionized water 1000ml, sterilizing.
In step 3), described fermentative medium formula is: molasses 15%, corn steep liquor 2%, MgSO
40.05%, K
2hPO
40.15%, NaCl0.1%, glucose 1.5%.
Embodiment 3
For a composite fungus agent for low temperature compost, form:
Rhodopseudomonas B6-15 2.0 * 10
11cfu/mL;
Rhodopseudomonas B5-16 1.5 * 10
11cfu/mL;
The total content value range of two kinds of bacterial strains is 3.0 * 10
11-2.0 * 10
12cfu/mL.
The preparation method of above-mentioned composite fungus agent, comprises the steps:
1) slant culture: by B6-15, be inoculated in respectively on solid medium under B5-16 original strain aseptic condition, cultivate 1 day under 20 ℃ of conditions, activation bacterial strain;
2) first order seed cultivation: by step 1) under the bacterial classification aseptic condition of activation, be inoculated in respectively NR liquid nutrient medium, under 20 ℃ of conditions, air flow 1:1.2,160r/min shaking table is cultivated 1 day, makes primary seed solution;
3) mixing fermentation culture: by step 2) primary seed solution B6-15, B5-16 is inoculated in fermentor tank according to 5% of fermention medium volume, cultivates 3 days, obtains composite fungus agent.
Wherein, in step 1), described solid culture based formulas is: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, agar 15g/L, deionized water 1000ml, sterilizing.
In step 3), described fermentative medium formula is: molasses 15%, corn steep liquor 2%, MgSO
40.05%, K
2hPO
40.15%, NaCl0.1%, glucose 1.5%.
Experimental example 1 strain growth situation is investigated
By Rhodopseudomonas B6-15, B5-16 respectively picking one articulating enter in 50ml liquid nutrient medium, in the shaking table of 20 ℃, rotating speed is 160r/min, cultivate 24h and make seed liquor, then respectively get mixed culture in the liquid nutrient medium that the above-mentioned seed liquor access of 1ml 100ml is fresh, cultured continuously in 20 ℃, 160r/min shaking table, every 4h measures the OD of liquid
600value, the results are shown in Table 1.
Wherein, beef extract-peptone liquid nutrient medium: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, deionized water 1000ml, sterilizing is standby.
The OD of table 1 composite fungus agent growth
600value
| ? | 0h | 4h | 8h | 12h | 16h | 20h | 24h |
| OD 600Value | 0 | 0.2 | 1.0 | 1.9 | 2.2 | 2.9 | 2.9 |
As shown in table 1, composite fungus agent can faster growing in 20 ℃ of environment, in 20h, substantially reaches stationary phase.
Stability and the pH of experimental example 2 composite fungus agents in the process of going down to posterity changes
Embodiment 1-3 composite fungus agent is inoculated in the 250mL Erlenmeyer flask that the bacterium NR substratum that gone out is housed by 1% inoculum size, under 20 ℃ of conditions, goes down to posterity and cultivate 24h and obtain s-generation bacterium liquid; The cultivation of in like manner going down to posterity for the third time.Go down to posterity altogether 20 times, measure respectively OD in the process that goes down to posterity
600value and pH, the results are shown in Table 2, table 3.
The stability of table 2 composite fungus agent
| ? | 5h | 15h | 24h | 3d | 10d | 15d | 20d |
| Embodiment 1 | 0.6 | 2.3 | 2.5 | 2.0 | 2.3 | 2.1 | 2.8 |
| Embodiment 2 | 0.5 | 2.1 | 2.4 | 2.5 | 2.2 | 2.7 | 2.8 |
| Embodiment 3 | 0.6 | 2.2 | 2.6 | 2.1 | 2.8 | 2.8 | 3.0 |
The pH of table 3 composite fungus agent
| ? | 5h | 15h | 24h | 3d | 10d | 15d | 20d |
| Embodiment 1 | 7.69 | 7.91 | 7.84 | 7.96 | 7.89 | 7.88 | 7.92 |
| Embodiment 2 | 7.96 | 8.00 | 7.85 | 7.98 | 7.74 | 7.73 | 7.73 |
| Embodiment 3 | 7.98 | 7.96 | 7.93 | 7.59 | 7.88 | 7.80 | 7.57 |
As shown in table 2, table 3, OD after cultivation 24h
600mean value is in 2.5 left and right, and pH value is the suitableeest at 7.50-8.00.
The application of experimental example 3 composite fungus agents in promoting compost temperature-rise period
Take bacterial strain B6-15, B5-16 as reference examples, carry out compost intensification experiment with embodiment 1-3 gained composite fungus agent.The material that uses chicken manure and sawdust to heat up and test as compost.Regulate compost original material usage quantity to make the initial C/N ratio of compost be about 25, moisture is about 60%, fully composting material is mixed, afterwards respectively by bacterial strain B6-15, B5-16, the 4 ‰ access composting materials of embodiment 1-3 gained composite fungus agent with composting material dry weight, after itself and composting material are mixed, pack into using foam box as compost fermentation reaction unit in, compost fermentation reaction unit is put in to envrionment temperature and in 2-4 ℃ of left and right indoor, carries out the compost experiment that heats up.From composting material, pack in fermentation reaction device and start timing, every 4h read and record in windrow, bottom temp once, average.The results are shown in Table 4.
Table 4 composite fungus agent and the experiment of reference examples compost
| ? | 0h | 4h | 8h | 18h | 26h | 40h | 45h |
| Embodiment 1 | 30℃ | 48.0℃ | 49.6℃ | 52.5℃ | 35.5℃ | 19.1℃ | 15.0℃ |
| Embodiment 2 | 30℃ | 48.0℃ | 50.5℃ | 53.0℃ | 39.0℃ | 22.0℃ | 18.5℃ |
| Embodiment 3 | 30℃ | 50.5℃ | 52.0℃ | 56.0℃ | 42.0℃ | 20.0℃ | 19.0℃ |
| Bacterial strain B6-15 | 30℃ | 47.5℃ | 47.5℃ | 48.0℃ | 45.5℃ | 27.5℃ | 26.0℃ |
| Bacterial strain B5-16 | 30℃ | 46.0℃ | 46.5℃ | 47.0℃ | 42.5℃ | 19.5℃ | 18.0℃ |
As shown in table 4, embodiment 1-3 gained composite fungus agent can effectively promote compost to be rapidly heated under the low temperature environment of 2-4 ℃, particularly the temperature after inoculation 18h of the composite fungus agent described in embodiment 3 reaches as high as 56 ℃, and single bacterial strain temperature after 4h is difficult to improve again, is only 48.0 ℃.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (9)
1. for a composite fungus agent for low temperature compost, it is characterized in that, by following bacterial strain, formed:
Rhodopseudomonas B6-15 1.0 * 10
10～1.0 * 10
12cfu/mL;
Rhodopseudomonas B5-16 1.0 * 10
10～1.0 * 10
12cfu/mL;
Total count is 2.0 * 10
10-2.0 * 10
12cfu/mL.
2. composite fungus agent according to claim 1, is characterized in that, following bacterial strain, consists of:
Rhodopseudomonas B6-15 2.6 * 10
10～20.0 * 10
10cfu/mL;
Rhodopseudomonas B5-16 1.8 * 10
10～15.0 * 10
10cfu/mL.
3. the preparation method of composite fungus agent described in claim 1 or 2, is characterized in that, comprises the steps:
1) slant culture: by B6-15, be inoculated in respectively on solid medium under B5-16 original strain aseptic condition, cultivate 1-2 days under 15-25 ℃ of condition, activation bacterial strain;
2) first order seed cultivation: by step 1) under the bacterial classification aseptic condition of activation, be inoculated in respectively NR liquid nutrient medium, 15-25 ℃ of shaking table cultivated 1-2 days, makes primary seed solution;
3) mixing fermentation culture: by step 2) primary seed solution B6-15, B5-16 is inoculated in fermentor tank mixed culture 3-5 days under 20 ℃ of conditions, obtains composite fungus agent.
4. preparation method according to claim 3, is characterized in that, in step 1), described solid culture based formulas is: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, agar 15g/L, deionized water 1000ml.
5. preparation method according to claim 3, is characterized in that, in step 2) in, described NR liquid culture based formulas is: peptone 10g/L, extractum carnis 5g/L, NaCl5g/L, deionized water 1000ml.
6. preparation method according to claim 3, is characterized in that, in step 2) in, described shaking table culture condition is: air flow 1:1-1.2,150-200r/min.
7. preparation method according to claim 3, is characterized in that, in step 3), fermentative medium formula is: molasses 15%, corn steep liquor 2%, MgSO
40.05%, K
2hPO
40.15%, NaCl0.1%, glucose 1.5%.
8. preparation method according to claim 3, is characterized in that, in step 3), inoculum size is the 1-10% of fermention medium volume.
9. the application of composite fungus agent in low temperature compost described in claim 1 or 2.
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| CN105294185A (en) * | 2015-11-06 | 2016-02-03 | 东北农业大学 | Preparation method of low-temperature agricultural compost temperature raising agent capable of deceasing agricultural non-point source pollution |
| CN105567591A (en) * | 2015-12-31 | 2016-05-11 | 北京沃土天地生物科技股份有限公司 | Low temperature anti-biological inoculant, solid state fermentation method and applications thereof |
| CN114958672A (en) * | 2022-06-06 | 2022-08-30 | 南京农业大学 | Compound microbial agent for rapid initiation of low-temperature compost as well as preparation method and application thereof |
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| CN104480051A (en) * | 2014-12-31 | 2015-04-01 | 北京沃土天地生物科技有限公司 | Low-temperature compost inoculant and preparation and application of low-temperature compost inoculant |
| CN104480051B (en) * | 2014-12-31 | 2019-10-29 | 北京沃土天地生物科技有限公司 | A kind of low temperature compost Inoculant and its preparation and application |
| CN105294185A (en) * | 2015-11-06 | 2016-02-03 | 东北农业大学 | Preparation method of low-temperature agricultural compost temperature raising agent capable of deceasing agricultural non-point source pollution |
| CN105294185B (en) * | 2015-11-06 | 2018-12-04 | 东北农业大学 | The agricultural compost of low temperature that can be reduced agricultural non-point source pollution plays the preparation method of warm agent |
| CN105567591A (en) * | 2015-12-31 | 2016-05-11 | 北京沃土天地生物科技股份有限公司 | Low temperature anti-biological inoculant, solid state fermentation method and applications thereof |
| CN114958672A (en) * | 2022-06-06 | 2022-08-30 | 南京农业大学 | Compound microbial agent for rapid initiation of low-temperature compost as well as preparation method and application thereof |
| CN114958672B (en) * | 2022-06-06 | 2024-03-01 | 南京农业大学 | Low-temperature composting rapid detonation composite microbial agent and preparation method and application thereof |
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