CN106938949A - A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer - Google Patents
A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer Download PDFInfo
- Publication number
- CN106938949A CN106938949A CN201710228881.6A CN201710228881A CN106938949A CN 106938949 A CN106938949 A CN 106938949A CN 201710228881 A CN201710228881 A CN 201710228881A CN 106938949 A CN106938949 A CN 106938949A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- organic fertilizer
- kitchen garbage
- bacillus subtilis
- high concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer, belong to organic fertilizer production field, key step includes：The aerobic degradation and two steps of inoculation of bacillus subtilis of kitchen garbage and husk mixture.On the one hand this method remains the high efficiency of kitchen garbage aerobic degradation, on the other hand improves the content of bacillus subtilis in organic fertilizer, and both the feature with organic fertilizer also contains a large amount of bacillus subtilises beneficial to plant growth to the organic fertilizer produced.
Description
Technical field
The present invention relates to organic fertilizer production field, particularly a kind of load high concentration bacillus subtilis kitchen garbage is organic
The preparation method of fertilizer.
Background technology
Kitchen garbage is production and the common biomass solid wastes produced in life, wherein including substantial amounts of easy drop
Solve organic matter, these organic emissions into environment after can in the presence of various microorganisms rapid corruption, produce substantial amounts of oozes
Filtrate, corrupt gas can also grow pathogen simultaneously, and the production and living to environment and people cause to have a strong impact on.
Aerobic degradation technology is to utilize thermophilic microorganism under the high temperature conditions（50~60 ℃）Kitchen garbage is converted rapidly
For the resource technology of organic fertilizer, this method has transformation efficiency high（Kitchen garbage is converted into organic fertilizer in 96 hours）,
The characteristics of product content of organic matter is high, but the environmental condition required for aerobic degradation technology is 50 ~ 60 DEG C, this temperature strip
Part can allow a large amount of thermophilic normal temperature microorganisms to be in dormancy or dead state, wherein comprising microorganism largely beneficial to plant growth,
Substantial amounts of thermophilic microorganism is included in the organic fertilizer produced under these conditions, after organic fertilizer is delivered into farmland, by
Changed in temperature environment, thermophilic microorganism is in inactivated state.Therefore, contain in the organic fertilizer produced by this way
The thermophilic normal temperature micro organism quantity having is extremely limited, and the microorganism beneficial to plant is also extremely limited.
Bacillus subtilis is common bacterium in a kind of nature, is distributed widely in soil, this microorganism is in life
Various active material can be discharged in long process and suppresses a variety of viruses and harmful microbe breeding and growth, while withered grass gemma
Bacillus can also discharge some active materials beneficial to plant growth, stimulate the growth of plant.Due to fitting for bacillus subtilis
Suitable growth temperature is 30 ~ 37 DEG C, therefore in the aerobic degradation under the conditions of 50 ~ 60 DEG C, bacillus subtilis can not be fine
Breeding, also constrain the number of viable of Bacillus subtillis in product.
The content of the invention
The purpose of the present invention be for it is above-mentioned exist problem there is provided one kind load high concentration bacillus subtilis kitchen garbage
The preparation method of organic fertilizer, the organic fertilizer that profit is produced in this way can not only realize kitchen garbage to the quick of organic fertilizer
Conversion, while the organic fertilizer produced is also containing a large amount of bacillus subtilises beneficial to plant growth.
Technical scheme：
A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer, comprises the following steps：
（1）Stalk is dried and 1 ~ 10mm is crushed to；Kitchen garbage draining that dining room is fetched, drain and dry as far as possible；
（2）By microorganism Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans
Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009,
Seven kinds of Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 are micro-
Bacterization is cultivated 24 hours in 500ml LB culture mediums under the conditions of 55 DEG C.Obtained microbial inoculum is mixed micro-
Bacteria agent A.The formula of wherein every liter LB culture medium is：Peptone 10g, dusty yeast 5g, sodium chloride 5g, pH value 7.4；
（3）By stalk and kitchen garbage according to 34:66 mass ratio mixing；Mixture is placed in model TBH-CSCL-40's
In good time moisturizing in aerobic degradation, aerobic degradation is carried out in kitchen garbage biochemical reactor, material water ratio is maintained between 55%
~ 60%, while adding microbial bacterial agent A into reactor.It is 20rpm that rotating speed of agitator is set in course of reaction, and temperature conditionss are
55 DEG C, material water ratio is between 55% ~ 60% in appropriate moisturizing maintenance reaction device；
（4）Bacillus subtilis is inoculated in 20 liters of LB fluid nutrient mediums, is placed in after being cultivated 2 days under normal temperature and obtains withered grass gemma
Bacillus bacterium solution；
（5）After aerobic degradation terminates, cancel system heating, by 20 L bacillus subtilis bacterium after material is cooled to normal temperature
Liquid is all added in material, and the temperature remains within the normal range persistently stirs and divulge information for reactor, until material is spontaneously dried, that is, is obtained containing greatly
Measure the organic fertilizer of bacillus subtilis viable bacteria.
The advantages of the present invention are：
（1）Advantage of the aerobic degradation technology to kitchen garbage rapid conversion is maintained, aerobic degradation hot environment is compensate for
Killing and inhibitory action to thermophilic normal temperature microorganism；
（2）Obtained organic fertilizer product had both had abundant organic fertilizer nutrition species, nutritive element content height, had not destroyed soil texture
The features such as, also containing a large amount of bacillus subtilis viable bacterias, this microorganism can provide nutrition and various lifes for the growth of plant
The long factor, prevents the generation of plant disease；
（3）Bacillus subtilis is comprised only in obtained organic fertilizer product, a large amount of bacillus subtilises are gathered in shape in organic fertilizer
The breeding of other microorganisms and pathogen can be suppressed into dominant population, product fertilizer efficiency is preserved for a long time；
（4）This mode of production is simple and easy to apply, under low cost, effect significantly, kitchen garbage large-scale industry metaplasia can be used as
Produce the template of efficient organic fertilizer.
Embodiment
The present invention is described in further detail by following examples, but the technology contents that are described of the present embodiment be it is illustrative,
Rather than it is limited, protection scope of the present invention should not be limited to according to this.
Embodiment：
A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer, comprises the following steps：
（1）Stalk is dried and 1 ~ 10mm is crushed to；Kitchen garbage draining that dining room is fetched, drain and dry as far as possible；
（2）By microorganism Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans
Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009,
Seven kinds of Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 are micro-
Bacterization is cultivated 24 hours in 500ml LB culture mediums under the conditions of 55 DEG C.Obtained microbial inoculum is mixed micro-
Bacteria agent A.The formula of wherein every liter LB culture medium is：Peptone 10g, dusty yeast 5g, sodium chloride 5g, pH value 7.4；
（3）By stalk and kitchen garbage according to 34:66 mass ratio mixing；Mixture is placed in model TBH-CSCL-40's
In good time moisturizing in aerobic degradation, aerobic degradation is carried out in kitchen garbage biochemical reactor, material water ratio is maintained between 55%
~ 60%, while adding microbial bacterial agent A into reactor.It is 20rpm that rotating speed of agitator is set in course of reaction, and temperature conditionss are
55 DEG C, material water ratio is between 55% ~ 60% in appropriate moisturizing maintenance reaction device；
（4）Bacillus subtilis is inoculated in 20 liters of LB fluid nutrient mediums, is placed in after being cultivated 2 days under normal temperature and obtains withered grass gemma
Bacillus bacterium solution；
（5）After aerobic degradation terminates, cancel system heating, by 20 L bacillus subtilis bacterium after material is cooled to normal temperature
Liquid is all added in material, and the temperature remains within the normal range persistently stirs and divulge information for reactor, until material is spontaneously dried, that is, is obtained containing greatly
Measure the organic fertilizer of bacillus subtilis viable bacteria.
Reaction is sampled after terminating from material, and the method according to standard NY525-2012 is carried out to organic fertilizer sample
Detection, test result indicate that every Testing index reaches or better than the requirement of defined in standard, wherein, TOC
For 88%, total nutrient content is 8.3%, and moisture content 21%, pH value is 7.3.Method according to standard NY/T 227-1994
Viable count in organic fertilizer sample is detected, testing result shows that the viable count in sample reaches 1.3 hundred million/g.
Claims (4)
1. a kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer, comprises the following steps：
（1）Stalk is dried and 1 ~ 10mm is crushed to；Kitchen garbage draining that dining room is fetched, drain and dry as far as possible；
（2）By microorganism Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans
Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009,
Seven kinds of Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 are micro-
Bacterization is cultivated 24 hours in 500ml LB culture mediums under the conditions of 55 DEG C, obtained microbial inoculum is mixed micro-
Bacteria agent A, wherein the formula of every liter of LB culture medium is：Peptone 10g, dusty yeast 5g, sodium chloride 5g, pH value 7.4；
（3）By stalk and kitchen garbage according to 34:66 mass ratio mixing；Mixture is placed in model TBH-CSCL-40's
In good time moisturizing in aerobic degradation, aerobic degradation is carried out in kitchen garbage biochemical reactor, material water ratio is maintained between 55%
~ 60%, set rotating speed of agitator for 20rpm while being added into reactor in microbial bacterial agent A, course of reaction, temperature conditionss are
55 DEG C, material water ratio is between 55% ~ 60% in appropriate moisturizing maintenance reaction device；
（4）Bacillus subtilis is inoculated in 20 liters of LB fluid nutrient mediums, is placed in after being cultivated 2 days under normal temperature and obtains withered grass gemma
Bacillus bacterium solution；
（5）After aerobic degradation terminates, cancel system heating, by 20 L bacillus subtilis bacterium after material is cooled to normal temperature
Liquid is all added in material, and the temperature remains within the normal range persistently stirs and divulge information for reactor, until material is spontaneously dried, that is, is obtained containing greatly
Measure the organic fertilizer of bacillus subtilis viable bacteria.
2. according to claim 1 it is a kind of load high concentration bacillus subtilis kitchen garbage organic fertilizer preparation method, its
It is characterised by：The manufacturing process of organic fertilizer uses aerobic thermophilic composting technology, and actual conditions is：Temperature is aqueous between 50 ~ 60 DEG C
Rate is between 55% ~ 65%, and agitating paddle and blower fan keep continuous running.
3. according to claim 1 it is a kind of load high concentration bacillus subtilis kitchen garbage organic fertilizer preparation method, its
It is characterised by：Microbial bacterial agent A is by Bacillus sp. MN-07, Bacillus coagulans, Bacillus
Coagulans strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain
Pb-WC09009, Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain
Seven kinds of microorganism groups of TS-11 into.
4. according to claim 1 it is a kind of load high concentration bacillus subtilis kitchen garbage organic fertilizer preparation method, its
It is characterised by：The microorganism of cooling stage inoculation is Bacillus subtillis zymocyte liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710228881.6A CN106938949A (en) | 2017-04-10 | 2017-04-10 | A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710228881.6A CN106938949A (en) | 2017-04-10 | 2017-04-10 | A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106938949A true CN106938949A (en) | 2017-07-11 |
Family
ID=59464551
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710228881.6A Pending CN106938949A (en) | 2017-04-10 | 2017-04-10 | A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106938949A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108203352A (en) * | 2018-03-13 | 2018-06-26 | 武汉理工大学 | A kind of production method of kitchen garbage organic fertilizer |
| CN108207812A (en) * | 2017-12-19 | 2018-06-29 | 奥为（天津）环保科技有限公司 | A kind of preparation method of vermiculture base-material |
| CN112462006A (en) * | 2020-11-27 | 2021-03-09 | 扬州鑫威环保生物科技有限公司 | Experiment method for culturing biological agent for kitchen waste treatment |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1460664A (en) * | 2003-05-19 | 2003-12-10 | 清华大学 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
| CN101538174A (en) * | 2009-01-20 | 2009-09-23 | 新疆惠森生物技术有限公司 | Method and special bacteria-adding equipment for adding bacteria after preparing bio-organic fertilizer |
| CN102951939A (en) * | 2012-11-02 | 2013-03-06 | 湖南回龙湖现代农业科技有限公司 | Rapid harmless plant organic residue treatment and fertilizer utilization process and equipment thereof |
| CN103980017A (en) * | 2014-05-17 | 2014-08-13 | 张鸿 | Method for rapidly producing high-quality organic fertilizer by virtue of high-temperature aerobic secondary fermentation |
| CN105152704A (en) * | 2015-07-09 | 2015-12-16 | 标优美生态工程股份有限公司 | Organic solid waste fast resource treatment process for industrial production |
-
2017
- 2017-04-10 CN CN201710228881.6A patent/CN106938949A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1460664A (en) * | 2003-05-19 | 2003-12-10 | 清华大学 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
| CN101538174A (en) * | 2009-01-20 | 2009-09-23 | 新疆惠森生物技术有限公司 | Method and special bacteria-adding equipment for adding bacteria after preparing bio-organic fertilizer |
| CN102951939A (en) * | 2012-11-02 | 2013-03-06 | 湖南回龙湖现代农业科技有限公司 | Rapid harmless plant organic residue treatment and fertilizer utilization process and equipment thereof |
| CN103980017A (en) * | 2014-05-17 | 2014-08-13 | 张鸿 | Method for rapidly producing high-quality organic fertilizer by virtue of high-temperature aerobic secondary fermentation |
| CN105152704A (en) * | 2015-07-09 | 2015-12-16 | 标优美生态工程股份有限公司 | Organic solid waste fast resource treatment process for industrial production |
Non-Patent Citations (2)
| Title |
|---|
| 江洋等: "餐厨垃圾好氧堆肥技术市场化推广与应用的关键问题分析 ", 《生态经济》 * |
| 王正银主编: "《肥料研制与加工》", 30 June 2011, 中国农业大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108207812A (en) * | 2017-12-19 | 2018-06-29 | 奥为（天津）环保科技有限公司 | A kind of preparation method of vermiculture base-material |
| CN108203352A (en) * | 2018-03-13 | 2018-06-26 | 武汉理工大学 | A kind of production method of kitchen garbage organic fertilizer |
| CN112462006A (en) * | 2020-11-27 | 2021-03-09 | 扬州鑫威环保生物科技有限公司 | Experiment method for culturing biological agent for kitchen waste treatment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hou et al. | Role of psychrotrophic bacteria in organic domestic waste composting in cold regions of China | |
| CN103232944B (en) | Microorganism bacterium agent for straw and excrement mixed composting | |
| CN106497836A (en) | A kind of complex micro organism fungicide of fermented stalk and its preparation method and application | |
| CN105036853B (en) | A kind of method that biological organic fertilizer is prepared using chicken manure fermenting | |
| CN106701603A (en) | Preparation method of high-efficient decay-promoting agent | |
| CN107141047A (en) | A kind of damp and hot pretreatment promotes the compost method that feces of livestock and poultry becomes thoroughly decomposed | |
| CN106978367B (en) | Bacillus ureafaciens strain TB42 and application thereof in promoting compost maturity | |
| CN101857469B (en) | Preparation process for golf course lawn slow-release bio-organic fertilizer produced by three continuous fermentations | |
| CN106966800A (en) | A kind of preparation method containing high concentration bacillus licheniformis organic fertilizer | |
| CN106544302A (en) | A kind of complex micro organism fungicide for soil improvement and preparation method thereof | |
| CN108018233A (en) | One plant of Siam bacillus and its preparation and application of microbial bacterial agent | |
| CN106938949A (en) | A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer | |
| CN106699474A (en) | Novel bio-organic fertilizer and preparation method thereof | |
| CN104651267B (en) | A kind of organic fertilizer with the microbial bacteria and its application of fermentation production alkali | |
| CN112760274B (en) | Organic solid waste high-temperature aerobic composting strain and application thereof | |
| CN104909846B (en) | A kind of decomposed leavening of compound chicken manure containing bacillus amyloliquefaciens HFJ 7 and its application | |
| CN110195027A (en) | The preparation method and application of nicotianae ZL-1 and its compost low temperature fermentation inoculum | |
| CN103540548B (en) | Compound fungicide for low-temperature compost, and preparation method and application thereof | |
| CN103205388B (en) | Method for producing viable bacteria of bacillus licheniformis by high-density solid fermentation | |
| CN107446866A (en) | A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement | |
| CN108277187A (en) | Microbial bacterial agent for municipal sludge high-performance bio desiccation | |
| CN103289912A (en) | Solid fermentation method of bacillus coagulans | |
| CN109609412A (en) | A kind of Thermophilic Bacteria Bacillus smithii Ths1 and its application | |
| CN106905063A (en) | A kind of preparation method for loading high concentration Paenibacillus polymyxa kitchen garbage organic fertilizer | |
| CN106986694A (en) | A kind of preparation method containing high concentration potassium solubilizing bacteria organic fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170711 |