CN103484372B - A kind of cultural method improving microalgae biomass and grease yield - Google Patents
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000002028 Biomass Substances 0.000 title claims abstract description 21
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- 235000013619 trace mineral Nutrition 0.000 claims abstract description 21
- 235000016709 nutrition Nutrition 0.000 claims abstract description 15
- 230000035764 nutrition Effects 0.000 claims abstract description 15
- 241000195493 Cryptophyta Species 0.000 claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 239000002609 medium Substances 0.000 claims abstract description 5
- 235000015097 nutrients Nutrition 0.000 claims abstract description 5
- 235000013343 vitamin Nutrition 0.000 claims description 6
- 239000011782 vitamin Substances 0.000 claims description 6
- 229930003231 vitamin Natural products 0.000 claims description 6
- 229940088594 vitamin Drugs 0.000 claims description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 6
- 241000224476 Nannochloropsis salina Species 0.000 claims description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 4
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 4
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 4
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 4
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 4
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 4
- 229910052927 chalcanthite Inorganic materials 0.000 claims description 4
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 4
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 4
- 239000011565 manganese chloride Substances 0.000 claims description 4
- 239000011684 sodium molybdate Substances 0.000 claims description 4
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 239000011686 zinc sulphate Substances 0.000 claims description 4
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 3
- 235000002639 sodium chloride Nutrition 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 2
- 229930003779 Vitamin B12 Natural products 0.000 claims description 2
- 229960002685 biotin Drugs 0.000 claims description 2
- 235000020958 biotin Nutrition 0.000 claims description 2
- 239000011616 biotin Substances 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 2
- 239000011715 vitamin B12 Substances 0.000 claims description 2
- 235000019163 vitamin B12 Nutrition 0.000 claims description 2
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 9
- 230000011218 segmentation Effects 0.000 abstract description 3
- 239000012141 concentrate Substances 0.000 abstract description 2
- 230000012010 growth Effects 0.000 description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 239000003225 biodiesel Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000014593 oils and fats Nutrition 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000005791 algae growth Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- -1 iron ion Chemical class 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000002803 fossil fuel Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of cultural method improving microalgae biomass and grease yield in algae bio culture technique field.The method that the present invention is cultivated by lean nutrition second order segmentation after microalgae is used first eutrophy, first by microdisk electrode in eutrophy culture medium to exponential phase, then concentrate and collect microalgae and be inoculated into without the lean Nutrient medium of trace element continuing cultivate.The present invention reduces cost without adding extra nutrition, and the yield of biomass of microalgae, cell grease content and final grease yield are greatly facilitated, and overcomes traditional nitrogen stress and cultivates and only increase cell grease content, but the shortcoming that final grease yield is the lowest.Under the conditions of scarce trace element, the fat content of microalgae has reached 40%, and biomass and grease yield have been respectively increased 65% and 133%.
Description
Technical field
The present invention relates to the culture technique of algae bio, particularly to a kind of cultural method improving microalgae biomass and grease yield.
Background technology
Constantly consuming and the mankind's continuous growth to energy demand of conventional fossil fuel, result in the most serious energy crisis.There are some researches show, counting coal, oil and natural gas in the world will about exhaust behind 107 years, 35 years and 37 years from 2005.Seek a kind of environmental protection, regenerative resource becomes countries in the world and needs a big problem of solution badly.Utilize microalgae to produce biodiesel and provide a solution with great potential.Microalgae contrasts with other biological, and because of its fast growth, floor space is little, training method is simple, and part algae contains the highest lubricant component and paid close attention to the most widely.Utilizing microalgae to produce biodiesel is considered as the currently the only a kind of bioenergy that can replace conventional traffic transport fuel.Therefore, in order to improve grease yield and reduce cost, current normal condition of culture and culture process need further optimized and improve.
Micro algae growth speed under normal growing conditions compares comparatively fast, but fat content but ratio is relatively low.Under the condition pressure that certain is special, microalgae tends to the oils and fats of enriched.Such as, and a report on " Bioresource technology " periodical in 2008 (volume 99,11 phases, 4717-4722 page) find, the iron ion of high concentration can induce microalgaeChlorella vulgarisProduce the oils and fats of high-load.But this method needs to increase the concentration of iron ion in culture fluid, not only increases cost but also can cause the pollution of water body.It is the most relatively common a kind of oils and fats enrichment method that nitrogen stress is cultivated, but the speed of growth of microalgae is greatly inhibited under these conditions, causes final grease yield to be improved the most significantly.Chinese patent " is supported and rich nitrogen-nitrogen stress two benches training strategy is improved oil-producing microalgae Biomass and the method for oil and fat accumulation " (patent No. 201110192159.4) and proposes at microalgae with holding concurrentlyScenedesmus dimorphusThe nitrogen stress stage is simultaneously introduced 5.4g/L glucose, improves microalgae biomass yield and fat content (reaching 29.62%).While it is true, this method is suitable only for the microalgae of row growth of can holding concurrently, and the addition of high concentration glucose adds cost.Therefore, how to design or find a kind of low cost, fat content can be improved again on the speed of growth affect the least cultural method become currently utilize microalgae produce biodiesel a big problem.
Summary of the invention
In order to solve the problem in background technology, the invention provides a kind of raising microalgae biomass and the cultural method of grease yield simultaneously, cultivated by dystrophic second order segmentation after first eutrophy, improve micro algae biomass and total grease yield.
Technical scheme: a kind of cultural method improving microalgae biomass and grease yield.First, microalgae is inoculated into normally rich in the culture medium of nutrition, cultivates to exponential phase.Then, algae solution is concentrated, be then seeded into without the lean Nutrient medium of trace element continuing cultivate.Without Na in the lean Nutrient medium of second stage2EDTA, FeCl3·6H2O, MnCl2·4H2O, CoCl2·6H2O, CuSO4·5H2O, ZnSO4·7H2O, Na2MoO4·2H2All trace element in O.
The present invention and prior art contrast have the advantages that the present invention uses dystrophic second order segmentation cultural method after first eutrophy, cost is reduced without adding extra nutrition, and the yield of biomass of microalgae, cell grease content and final grease yield are greatly facilitated.Overcome traditional nitrogen stress to cultivate and only increase cell grease content, but the shortcoming that final grease yield is the lowest.Under the conditions of scarce trace element, the fat content of microalgae has reached 40%, and biomass and grease yield have been respectively increased 65% and 133%.
Accompanying drawing explanation
Fig. 1 is the process flow diagram of the present invention.
Fig. 2 be micro-plan ball algae under the conditions of Different Nutrition in embodiments of the invention (N.salina) growth curve.
Fig. 3 be micro-plan ball algae under the conditions of Different Nutrition in embodiments of the invention (N.salina) fat content.
Fig. 4 be micro-plan ball algae under the conditions of Different Nutrition in embodiments of the invention (N.salina) grease yield.
Detailed description of the invention
Embodiment 1. the present embodiment micro-plan ball algae of raising (Nannochloropsis salina) cultural method of biomass and grease yield comprises the following steps that (process chart sees Fig. 1):
1. the eutrophy of microalgae is cultivated: by micro-plan ball algaeN.salinaBeing inoculated into normal rich in the f/2 culture medium of nutrition, initial pH is 8, temperature 24 ± 2oC, illumination 79.29 mol m-2
s-1, aerobic culture is to exponential phase.Rich in the f/2 culture medium of nutrition, every liter contains: the 33.6 artificial sea salt of g, 0.075 g NaNO3, 0.00565 g NaH2PO4·2H2O, 1 ml trace element (T) and 1 ml vitamin.Wherein, every liter of trace element T contains: 4.16 g Na2EDTA, 3.15 g FeCl3·6H2O, 0.18 g MnCl2·4H2O, 10 mg CoCl2·6H2O, 10 mg CuSO4·5H2O, 22 mg ZnSO4·7H2O, 6 mg Na2MoO4·2H2O.Every liter of vitamin contains: 100 mg vitamin B1s, 0.5 mg vitamin B12 and 0.5 mg biotin.
2. the collection of microalgae concentrates: treat micro-plan ball algaeN.salinaCultivate to logarithmic growth after date, algae solution medium-speed centrifuge is concentrated.
3. the lean nutrition of microalgae is cultivated: the microalgae after concentrating is inoculated in dystrophic f/2 culture medium, continues to cultivate 3 days.Use three kinds of different lean Nutrient medium to contrast, do not contain nitrate (nitrogen stress), not phosphate-containing (phosphorus deficiency) respectively or lack trace element without trace element T(), remaining component is identical with normal eutrophy f/2 culture medium.
Result shows, when lean nutrition cultivation stage, compared with original microdisk electrode, the micro algae growth Similar Broken Line of nitrogen stress, the biomass of phosphorus deficiency improve only 30%, and the microalgae biomass lacking trace element has the biggest raising (improve 65%, see Fig. 2).In terms of cell dry matter fat content, the microalgae of nitrogen stress, phosphorus deficiency and scarce trace element has respectively reached 38%, 46% and 40% (Fig. 3).In terms of final grease yield, compared with original microdisk electrode, the microalgae of nitrogen stress, phosphorus deficiency and scarce trace element has been respectively increased 42%, 117% and 133% (Fig. 4).Therefore, this example further demonstrate that the grease yield not significantly improving microalgae is cultivated in the lean nutrition of nitrogen stress.Although the fat content of phosphorus deficiency the highest (having reached 46%), but only improve 30% due to biomass content, so total grease yield still is below lacking trace element.By contrast, the microalgae lacking trace element but has obvious advantage, and its biomass and total grease yield are all the highest in all conditions, have been respectively increased 65% and 133% than biomass and the grease yield of original microalgae.Therefore, the training method lacking trace element after first eutrophy can either increase substantially yield of biomass, can guarantee that again higher cell grease content, thus improves total grease yield.
Claims (3)
1. one kind improve the raw micro-plan ball algae of salt (Nannochloropsis salina) biomass and the cultural method of grease yield, it is characterised in that microalgae carrying out successively two-period form and cultivates, after i.e. first eutrophy is cultivated, lean nutrition is cultivated, and described method includes:
(1) microalgae is inoculated into normally rich in the culture medium of nutrition, cultivates to exponential phase；
(2) then, algae solution is concentrated, be then seeded into without the lean Nutrient medium of trace element continuing cultivate.
Cultural method the most according to claim 1, the wherein said normal culture medium rich in nutrition, it is characterised in that every liter of culture medium contains: the 33.6 artificial sea salt of g, 0.075 g NaNO3, 0.00565 g NaH2PO4·2H2O, 1 ml trace element and 1 ml vitamin；Wherein, every liter of trace element contains: 4.16 g Na2EDTA, 3.15 g FeCl3·6H2O, 0.18 g MnCl2·4H2O, 10 mg CoCl2·6H2O, 10 mg CuSO4·5H2O, 22 mg ZnSO4·7H2O, 6 mg Na2MoO4·2H2O；Every liter of vitamin contains: 100 mg vitamin B1s, 0.5 mg vitamin B12 and 0.5 mg biotin.
Cultural method the most according to claim 2, wherein said refers to without the trace element part in culture medium without trace element, i.e. without Na2EDTA, FeCl3·6H2O, MnCl2·4H2O, CoCl2·6H2O, CuSO4·5H2O, ZnSO4·7H2O, Na2MoO4·2H2All trace element in O.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105647825B (en) * | 2014-11-13 | 2019-05-21 | 中国科学院大连化学物理研究所 | Method that is a kind of while improving spiral algal biomass and polysaccharide yield |
| CN105132404B (en) * | 2015-10-12 | 2021-03-02 | 山东大学 | Method for rapidly accumulating grease by utilizing ultrasonic stimulation microalgae |
| CN105858894A (en) * | 2016-02-19 | 2016-08-17 | 南昌大学 | Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen |
| CN106635810B (en) * | 2016-12-06 | 2020-02-14 | 沈阳化工研究院有限公司 | Microalgae and culture method of microalgae |
| CN107586720A (en) * | 2017-09-28 | 2018-01-16 | 新奥科技发展有限公司 | Microalgae culture method |
| CN110229755A (en) * | 2019-06-17 | 2019-09-13 | 昆明理工大学 | A method of promote bloom nitrogen stress to coerce lower microalgae grease using epiphysin and accumulates |
| CN113462578A (en) * | 2021-08-26 | 2021-10-01 | 海南绿藻世界生物科技有限公司 | Microalgae culture medium and culture method |
| CN116769847B (en) * | 2023-08-09 | 2023-12-05 | 德默特生物科技(珠海)有限公司 | Method for improving EPA content in pseudo-microalgae oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268377A (en) * | 2011-07-11 | 2011-12-07 | 江南大学 | Method for improving lipid producing microalga biomass and lipid accumulation with two stage culture strategy of mixotrophic and nitrogen-rich-nitrogen-deficient culture |
| CN103146581A (en) * | 2013-02-28 | 2013-06-12 | 大连理工大学 | Marine Nannochloropsis oceanica strain containing rich hexadecadienoic acid and culture method thereof capable of enhancing biomass and oil content |
-
2013
- 2013-10-01 CN CN201310463435.5A patent/CN103484372B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268377A (en) * | 2011-07-11 | 2011-12-07 | 江南大学 | Method for improving lipid producing microalga biomass and lipid accumulation with two stage culture strategy of mixotrophic and nitrogen-rich-nitrogen-deficient culture |
| CN103146581A (en) * | 2013-02-28 | 2013-06-12 | 大连理工大学 | Marine Nannochloropsis oceanica strain containing rich hexadecadienoic acid and culture method thereof capable of enhancing biomass and oil content |
Non-Patent Citations (6)
| Title |
|---|
| effect of nutirent limitation on fatty acid and lipid content of marine microalgae;Kiell Inge Reitan et al;《J.Phycol》;19941231;第30卷;972-979 * |
| Microalgae for oil strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor;Liliana Rodolfi et al;《Biotechnology and Bioengineering》;20091231;第102卷(第1期);材料与方法部分、图4、图5 * |
| 两步培养法提高蛋白核小球藻的油脂含量;黄冠华 等;《华南理工大学学报》;20081231;第36卷(第12期);97-101 * |
| 微藻油脂含量和组分及其影响因子的研究;孙珊 等;《海洋科学》;20091231;第33卷(第12期);第124页左栏第9-10行 * |
| 营养元素和pH对若夫小球藻生长和油脂积累的影响;杨勋 等;《南方水产科学》;20130831;第9卷(第4期);33-38 * |
| 营养元素限制对微芒藻Y_002油脂积累的影响;辜博 等;《热带作物学报》;20111231;第32卷(第5期);789-795 * |
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