CN103466807A - Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora - Google Patents
Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora Download PDFInfo
- Publication number
- CN103466807A CN103466807A CN2013104058933A CN201310405893A CN103466807A CN 103466807 A CN103466807 A CN 103466807A CN 2013104058933 A CN2013104058933 A CN 2013104058933A CN 201310405893 A CN201310405893 A CN 201310405893A CN 103466807 A CN103466807 A CN 103466807A
- Authority
- CN
- China
- Prior art keywords
- analog compound
- anthraquinone analog
- volume
- liquid
- grams
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora, relating to the field of bioengineering. The technique comprises the following steps: by using Ceriporiopsis subvermispora as the initial strain, carrying out liquid shake culture and liquid strain amplification culture, transferring the strain solution into the wastewater containing anthraquinone compounds, supplementing certain nutrient substances, and carrying out fermentation culture. By using the technique, the removal rate of the anthraquinone compounds can reach 60-100%.
Description
Technical field
The present invention relates to technical field of bioengineering, refer in particular to and utilize worm to intend the waste water that the degraded of wax bacterium contains anthraquinone analog compound.Raw wastewater is concentrated or is diluted to Anthraquinone after 1～50 grams per liter, the supplemental medium composition, the access worm is intended the anthraquinone analog compound in wax bacterium strain degradation waste water.After worm is intended the degraded of wax bacterium, the clearance of anthraquinone analog compound can reach 60～100%.
Background technology
Anthraquinone compounds is of a great variety, is important industrial chemicals and intermediate, bright-colored, scope is wide, thereby widespread use in the industries such as weaving, printing and dyeing, not only can be used for the printing and dyeing of terylene and BLENDED FABRIC, also can be used for the printing and dyeing of polyamide fibre, acrylic fibers, polypropylene fibre, vinegar fibre, wool, silk.Along with the lifting day by day of environmental requirement, azoic dyestuff forbidding range extension, also make the importance of anthraquinone dye more outstanding, makes its application in printing and dyeing industry more and more extensive simultaneously.But not only the factory effluent water yield is large for anthraquinone analog compound, and this compound colourity is high, and solubleness is low! Fusing point is high! A stable in properties! Be difficult to biological degradation, have again higher lipotropy, in the sewage directly discharged, anthraquinone is by sedimentation! But the effect prolonged stays such as food chain enrichment are in water body! Soil! In settling and air.Therefore explore the effectively technology of degraded anthraquinone analog compound, be of great significance for social harmony and development tool.
At present about the processing patent that contains anthraquinone analog compound waste water, have several, but main method is recycling, microbiological deterioration and the oxidation removal of anthraquinone.As patent " 1; improvement and its recovery method as resource of 4-dihydroxyanthraquinone factory effluent " (application number 00112386.6) discloses and has utilized macroporous resin adsorption, resolve with sodium hydroxide the method for recycling the anthraquinone analog compound in Isosorbide-5-Nitrae-dihydroxyanthraquinone factory effluent again; Patent " recovery method of a kind of Isosorbide-5-Nitrae-dihydroxyanthraquinone processing wastewater " (application number 200410013484.X) discloses the method for utilizing concentrated, cooling, crystallization, separation and the technology recycling anthraquinone such as dry.Patent " Sphingomonas strain and the application in the anthraquinone dye wastewater decolouring thereof " (application number 200410020832.6) discloses Sphingomonas, application in the anthraquinone dye wastewater decolouring, this bacterium decoloring ability is strong, degradation speed is fast, but can only be suitable for lower concentration anthraquinone waste water; Adopt in addition electrochemical oxidation (patent No. 200910029887.6) and photochemical catalytic oxidation (200710011629.6), these two kinds of methods also need further processing.Paper relevant to these methods also has many reports.Other doctor's or master's degree paper has reported that Phanerochaete chrysosporium absorption degradation anthraquinone analog compound mechanism is studied, but does not relate to its Technology.
The inventor is through deep research, find out a kind of with worm intend the wax bacterium (
ceriporiopsis subvermispora) as starting strain, the method that degraded contains anthraquinone analog compound waste water.The method that the method is different from the past is that used bacterial classification is directly to process the waste liquid (1～50 grams per liter) containing the high density anthraquinone analog compound by the method for fermentation, has saved diluting water and place takies.
Summary of the invention
The present invention is to provide worm and intend the biotechnology of anthraquinone analog compound in wax bacterium degrading waste water.Waste water is after worm is intended the degraded of wax bacterium, and its anthraquinone analog compound clearance can arrive 60 ~ 100%.
A kind of worm of the present invention is intended wax bacterium degraded anthraquinone analog compound Technology, according to following step, carry out: it is starting strain that the worm of take is intended the wax bacterium, carry out test tube enlarged culturing, liquid shaking bottle cultivation, thalline fluid enlargement culture, centrifugal collection thalline, then by anthraquinone analog compound in fermentative degradation waste water.
The present invention's bacterial classification used is any strain bacterial classification that worm is intended the wax bacterium, as the bacterial classification of Chinese agriculture microbial strains preservation administrative center (ACCC) preservation.
The applicable waste water of the present invention is any one waste water that contains anthraquinone analog compound, extracts the waste liquid of anthraquinone analog compound as the waste liquid of fermentative Production anthraquinone analog compound, from plant or take the waste liquid of anthraquinone analog compound as synthetic other products of raw material.
Test tube enlarged culturing base in wherein said test tube enlarged culturing is potato sucrose substratum (Zhu Gejian, Wang Zhengxiang chief editor's " industrial microorganism experimental technique handbook ", 1994,367 pages).
During wherein said liquid shaking bottle is cultivated and liquid shaking bottle substratum and liquid spawn culture medium in the thalline fluid enlargement culture be: glucose 5～30 grams per liters, peptone 0～5 grams per liter, yeast extract paste 0～5 grams per liter, sal epsom 0.2～1 grams per liter, tween 80 0.5～10 grams per liter, potassium primary phosphate 2～9 grams per liters, pH5～8,120～140 ℃ sterilizing 20～40 minutes; Wherein said shake-flask culture processing condition are, inoculation 3-5 piece worm is intended wax bacterium test tube slant thalline (5 * 5mm) in the 250mL triangular flask that 40～120 mL substratum are housed, at rotating speed: 150 rev/mins, 25～35 ℃, cultivate 24～72 hours; Wherein said first order seed culture process is, by 1～20%(volume ratio) inoculum size the shake-flask culture seed liquor is inoculated in the first order seed substratum, 25～35 ℃ of temperature, 50～200 rev/mins of mixing speed, air flow 0.2～2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 18～72 hours;
In degrading waste water of the present invention, the technique of anthraquinone analog compound is, the waste water that contains anthraquinone analog compound makes anthraquinone analog compound concentration reach 1～50 grams per liter waste water 1000L, 5～30 kilograms of glucose, 0.5～2 kilogram of soybean cake powder through concentrated or dilution; Copper sulfate 5～15 grams, ferrous sulfate 5～15 grams, manganous sulfate 5～20 grams, each composition of this substratum can amplify in this ratio; Wherein said degradation technique is: by 2～20%(seed liquor volume/wastewater volume) inoculation level liquid bacterial classification, 25～35 ℃ of temperature, 50～200 rev/mins of mixing speed, air flow 0.2～2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 36～168 hours.
After using this processing wastewater degraded, the anthraquinone analog compound clearance can reach 60-100%.
According to fermentating culturing process of the present invention, in conjunction with the ABC of this area, can be according to need of production, increase even level Four seeding tank of secondary, three grades, further expand the scale of production, to carry out suitability for industrialized production.Secondary, the three grades substratum that even the level Four seeding tank adopts are identical with the first order seed substratum with the shake-flask culture base, and inoculum size is 5～20%, and culture condition is same as liquid thalline enlarged culturing condition.
Embodiment
According to the anthraquinone analog compound measuring method of this process using, be: accurate absorption 1,8-dihydroxyanthraquinone reference substance solution (0.522 mg/ml) 1,3,5,8,10,15 ml, put respectively in the 25ml volumetric flask, fling to methyl alcohol, residue is with 0.5% magnesium acetate dissolve with ethanol solution and be diluted to scale, shake up, in 425 nm places, make reference with 0.5% magnesium acetate ethanolic soln and measure optical density.With 1,8-dihydroxy-anthracene quinone content (y), optical density (x) is carried out to linear regression analysis, obtain equation of linear regression.The accurate sample solution of drawing repeats aforesaid operations mensuration optical density, utilizes equation of linear regression to calculate the content of general anthraquinone in each sample.
Anthraquinone analog compound clearance Re calculates by following formula (1):
In formula,
r t clearance for t time anthraquinone analog compound;
c 0 (mg/L) be the anthraquinone analog compound concentration of 0 hour;
c t (mg/L) for adsorbing the concentration of t hour anthraquinone analog compound.
In order further to set forth related material and the technique of technical scheme of the present invention, provided following examples, but these embodiment do not limit the scope of the invention in any form.
Embodiment 1
1. the making of test tube slant bacterial classification
Access one ring worm plan wax bacterium bacterial classification in the new potato sucrose substratum configured (Zhu Gejian, Wang Zhengxiang chief editor's " industrial microorganism experimental technique handbook ", 1994,367 pages) (
ceriporiopsis subvermispora) (Chinese agriculture microbial strains preservation administrative center ACCC31512), 26 ℃, incubation time 50 hours; 4 ℃ save backup.
2. liquid shaking bottle is cultivated the making of bacterial classification
Accurately take glucose 5 grams, peptone 0 gram, yeast extract paste 5 grams, sal epsom 0.2 gram, tween 80 0.5 gram, potassium primary phosphate 2 grams, water 1000 mL, pH 5, packing 250mL triangular flask, every bottle of 50 grams, totally 20 bottles, 120 ℃ of sterilizings 40 minutes; After cooling, 3 worms of every bottle graft kind are intended wax bacterium test tube slant thalline (5 * 5mm), at 25 ℃, 150 rev/mins, cultivate 72 hours;
3. the making of level liquid bacterial classification
Accurately take glucose 50 grams, peptone 0 gram, yeast extract paste 50 grams, sal epsom 2 grams, tween 80 5 grams, potassium primary phosphate 20 grams, water 10L, be loaded in the seeding tank of 15L, 120 ℃ of sterilizings 20 minutes; After sterilizing is cooling, 25 ℃ of temperature, 50 rev/mins of mixing speed, 0.2: 1 volume/volume of air flow/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 72 hours;
4. wastewater degradation
Take rheum officinale as raw material, and the waste liquid process that adopts decocting method to extract anthraquinone analog compound concentrates, the waste liquid 1000L that anthraquinone analog compound concentration is 1 gram/L, 5 kilograms of glucose, 0.5 kilogram of soybean cake powder; Copper sulfate 5 grams, ferrous sulfate 5 grams, manganous sulfate 5 grams; Access 2%(seed liquor volume/wastewater volume) inoculation level liquid bacterial classification, 25 ℃ of temperature, 60 rev/mins of mixing speed, air flow 0.3:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate after 44 hours, the anthraquinone analog compound clearance reaches 60.2%.
Embodiment 2
1. the making of test tube slant bacterial classification
Potato sucrose substratum (Zhu Gejian, Wang Zhengxiang chief editor's " industrial microorganism experimental technique handbook " in new configuration, 1994,367 pages) middle access one ring worm plan wax bacterium bacterial classification (Chinese agriculture microbial strains preservation administrative center ACCC32476), 30 ℃, incubation time 100 hours; 7 ℃ save backup.
2. liquid shaking bottle is cultivated the making of bacterial classification
Accurately take glucose 150 grams, peptone 25 grams, yeast extract paste 25 grams,, sal epsom 0.4 gram, tween 80 4 grams, potassium primary phosphate 5 grams, water 10 L, pH 6, packing 250mL triangular flask, every bottle of 100 grams, totally 100 bottles, 130 ℃ of sterilizings 30 minutes; After cooling, 4 worms of every bottle graft kind are intended wax bacterium test tube slant thalline (5 * 5mm), at 30 ℃, 150 rev/mins, cultivate 50 hours;
3. the making of level liquid bacterial classification
Accurately take glucose 2000 grams, peptone 250 grams, yeast extract paste 250 grams, sal epsom 60 grams, tween 80 60 grams, potassium primary phosphate 400 grams, water 100L, be loaded in the seeding tank of 130L, 130 ℃ of sterilizings 30 minutes; After cooling, 30 ℃ of temperature, 125 rev/mins of mixing speed, air flow 1:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 50 hours;
4. wastewater degradation
The Fusarium oxysporum fermentation prepares the waste liquid of anthraquinone analog compound, reaches the waste water 1000L of 0.2 gram/L through concentrating the content that makes anthraquinone analog compound, 20 kilograms of glucose, 1.2 kilograms of soybean cake powders; Copper sulfate 10 grams, ferrous sulfate 10 grams, manganous sulfate 12 grams; Press 13%(seed liquor volume/wastewater volume) inoculation level liquid bacterial classification, 30 ℃ of temperature, 120 rev/mins of mixing speed, air flow 1:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 100 hours.Now the anthraquinone analog compound clearance reaches 80.3%.
Embodiment 3
1. the making of test tube slant bacterial classification
Potato sucrose substratum (Zhu Gejian, Wang Zhengxiang chief editor's " industrial microorganism experimental technique handbook " in new configuration, 1994,367 pages) middle access one ring worm plan wax bacterium bacterial classification (Chinese agriculture microbial strains preservation administrative center ACCC32475), 35 ℃, incubation time 144 hours; 10 ℃ save backup.
2. liquid shaking bottle is cultivated the making of bacterial classification
Accurately take glucose 300 grams, peptone 50 grams, yeast extract paste 0 gram, sal epsom 10 grams, tween 80 100 grams, potassium primary phosphate 90 grams, water 10L, pH7.5, packing 250mL triangular flask, every bottle of 120 mL, totally 80 bottles, 140 ℃ of sterilizings 20 minutes; After cooling, 5 worms of every bottle graft kind are intended wax bacterium test tube slant thalline (5 * 5mm), at 35 ℃, 150 rev/mins, cultivate 72 hours;
3. the making of level liquid bacterial classification
Accurately take glucose 1500 grams, peptone 250 grams, yeast extract paste 0 gram, sal epsom 50 grams, tween 80 500 grams, potassium primary phosphate 450 grams, water 50L, be loaded in the first class seed pot of 70L, 140 ℃ of sterilizings 40 minutes; After sterilizing is cooling, 35 ℃ of temperature, 200 rev/mins of mixing speed, air flow 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 50 hours;
4. the making of second-class liquid isolate
Accurately take glucose 15000 grams, peptone 2500 grams, yeast extract paste 0 gram, sal epsom 500 grams, tween 80 5000 grams, potassium primary phosphate 4500 grams, water 500L, be loaded in the secondary seed tank of 700L, 140 ℃ of sterilizings 40 minutes; After sterilizing is cooling, 35 ℃ of temperature, 200 rev/mins of mixing speed, air flow 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 18 hours;
5. wastewater degradation
Take anthraquinone analog compound as raw material, under the effect of catalyzer, the waste liquid of synthesizing nitryl anthraquinone, the waste liquid 1000L that to be diluted to Anthraquinone be 0.05 gram/L, 30 kilograms of glucose, 1.8 kilograms of soybean cake powders; Copper sulfate 15 grams, ferrous sulfate 15 grams, manganous sulfate 20 grams, press 20%(seed liquor volume/wastewater volume) inoculation level liquid bacterial classification, 34 ℃ of temperature, 190 rev/mins of mixing speed, air flow 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 168 hours.The anthraquinone analog compound clearance reaches 100%.
Claims (9)
1. a worm is intended wax bacterium degraded anthraquinone analog compound waster water process technology, it is characterized in that carrying out according to following step: it is starting strain that the worm of take is intended the wax bacterium, carry out test tube enlarged culturing, liquid shaking bottle cultivation, thalline fluid enlargement culture, liquid fermenting is removed anthraquinone analog compound in waste water, and the anthraquinone analog compound clearance can reach 60～100%.
2. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound waster water process technology, and its feature is any strain bacterial classification that worm is intended the wax bacterium at bacterial classification used.
3. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound Technology, its feature is any one waste water that contains anthraquinone analog compound at applicable waste water, extracts the waste liquid of anthraquinone analog compound as the waste liquid of fermentative Production anthraquinone analog compound, from plant or take the waste liquid of anthraquinone analog compound as synthetic other products of raw material.
4. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound Technology, and its feature enlarged culturing base in described test tube enlarged culturing therein is the potato sucrose substratum.
5. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound Technology, its feature during described liquid shaking bottle is cultivated therein and liquid shaking bottle substratum and liquid thalline substratum in the thalline fluid enlargement culture be: glucose 5～30 grams per liters, peptone 0～5 grams per liter, yeast extract paste 0～5 grams per liter, sal epsom 0.2～1 grams per liter, tween-80 0.5～10 grams per liter, potassium primary phosphate 2～9 grams per liters, pH5～8,120～140 ℃ sterilizing 20～40 minutes.
6. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound Technology, its feature described shake-flask culture processing condition therein is, inoculation 3-5 piece worm is intended wax bacterium test tube slant thalline (5 * 5mm) in the 250mL triangular flask that 40～120 mL substratum are housed, at rotating speed: 150 rev/mins, 25～35 ℃, cultivate 24～72 hours.
7. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound Technology, its feature described yeast culture technique therein is, by 1～20%(volume ratio) inoculum size the shake-flask culture seed liquor is inoculated in liquid thalline enlarged culturing, 25～35 ℃ of temperature, 50～200 rev/mins of mixing speed, air flow 0.2～2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 18～72 hours.
8. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound waster water process technology, its feature in the described anthraquinone analog compound wastewater medium that contains is: the waste water that contains anthraquinone analog compound makes anthraquinone analog compound concentration reach 1～50 grams per liter waste water 1000L through concentrated or dilution, 5～30 kilograms of glucose, 0.5～2 kilogram of soybean cake powder; Copper sulfate 5～15 grams, ferrous sulfate 5～15 grams, manganous sulfate 5～20 grams, each composition of this substratum can amplify in this ratio.
9. a kind of worm according to claim 1 is intended wax bacterium degraded anthraquinone analog compound waster water process technology, its feature described degrading waste water technique therein is: by 2～20%(seed liquor volume/wastewater volume) inoculation level liquid bacterial classification, 25～35 ℃ of temperature, 50～200 rev/mins of mixing speed, air flow 0.2～2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 36～168 hours, i.e. the degradable anthraquinone analog compound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013104058933A CN103466807A (en) | 2013-09-09 | 2013-09-09 | Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013104058933A CN103466807A (en) | 2013-09-09 | 2013-09-09 | Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103466807A true CN103466807A (en) | 2013-12-25 |
Family
ID=49791895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013104058933A Pending CN103466807A (en) | 2013-09-09 | 2013-09-09 | Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103466807A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5091089A (en) * | 1990-09-06 | 1992-02-25 | Development Center For Biotechnology | Microbial decolorization of wastewater |
| CN1618953A (en) * | 2004-06-24 | 2005-05-25 | 大连理工大学 | Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour |
| CN1792890A (en) * | 2005-11-16 | 2006-06-28 | 华东师范大学 | Process and apparatus for treating active dyeing waste water by white rot fungi biological membrane method |
| CN101734800A (en) * | 2008-11-07 | 2010-06-16 | 中国科学院沈阳应用生态研究所 | Method for decolorizing printing and dyeing waste water by adopting immobilized fungal mycelia |
| CN101851586A (en) * | 2010-05-11 | 2010-10-06 | 林永慧 | Fungus for efficiently decomposing synthetic dye and application thereof |
| CN102674560A (en) * | 2012-05-29 | 2012-09-19 | 江苏大学 | Process for biodegrading yellow water of white spirit factory |
-
2013
- 2013-09-09 CN CN2013104058933A patent/CN103466807A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5091089A (en) * | 1990-09-06 | 1992-02-25 | Development Center For Biotechnology | Microbial decolorization of wastewater |
| CN1618953A (en) * | 2004-06-24 | 2005-05-25 | 大连理工大学 | Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour |
| CN1792890A (en) * | 2005-11-16 | 2006-06-28 | 华东师范大学 | Process and apparatus for treating active dyeing waste water by white rot fungi biological membrane method |
| CN101734800A (en) * | 2008-11-07 | 2010-06-16 | 中国科学院沈阳应用生态研究所 | Method for decolorizing printing and dyeing waste water by adopting immobilized fungal mycelia |
| CN101851586A (en) * | 2010-05-11 | 2010-10-06 | 林永慧 | Fungus for efficiently decomposing synthetic dye and application thereof |
| CN102674560A (en) * | 2012-05-29 | 2012-09-19 | 江苏大学 | Process for biodegrading yellow water of white spirit factory |
Non-Patent Citations (5)
| Title |
|---|
| DIRK WESENBERG等: "White-rot fungi and their enzymes for the treatment of industrial dye effluents", 《BIOTECHNOLOGY ADVANCES》 * |
| JANJA BABIČ等: "Production of Ligninolytic Enzymesby Ceriporiopsis subvermispora for Decolourization of Synthetic Dyes", 《ACTA CHIM. SLOV.》 * |
| 张晓晴: "白腐真菌处理染料废水的研究", 《中国优秀硕士学位论文全文数据库（工程科技Ⅰ辑）》 * |
| 徐成勇等: "白腐菌对染料脱色和降解作用的研究进展", 《生物工程进展》 * |
| 黄民生等: "白腐真菌生物膜反应器处理染料生产废水实验研究", 《上海环境科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lu et al. | Brewery wastewater treatment and resource recovery through long term continuous-mode operation in pilot photosynthetic bacteria-membrane bioreactor | |
| CN105132323B (en) | One plant of salt tolerant bacillus and its application in high-salt wastewater processing | |
| CN103740609B (en) | The microorganism of one strain high-yield of 1,3-propanediol | |
| CN102268378B (en) | Method for screening high yield strains from aerobic bacteria at high flux | |
| CN106554931A (en) | One plant of Bai Shi carboxylics bacterium and its application | |
| CN103482770A (en) | Technology for degrading anthraquinone compounds in wastewater by use of phanerochaete chrysosporium | |
| CN106011002A (en) | Bacillus megatherium T317, microbial agent and preparation method of microbial agent | |
| Jin et al. | A bioprocessing mode for simultaneous fungal biomass protein production and wastewater treatment using an external air‐lift bioreactor | |
| Zhao et al. | Enhancement of COD, ammonia, phosphate and sulfide simultaneous removal by the anaerobic photosynthetic bacterium of Ectothiorhodospira magna in batch and sequencing batch culture | |
| CN101338289A (en) | Method for producing bio decolorizing strain agent special for dyeing waste water dye by fermenting alcohol waste water | |
| CN114456988B (en) | Functional flora for producing kaempferol and preparation and application thereof | |
| CN115896179A (en) | Method for producing high-added-value product by utilizing brewing wastewater from monascus makinoi extremely | |
| CN103466807A (en) | Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora | |
| CN103482772B (en) | Process technology for removing anthraquinone compounds out of wastewater through phanerochaete chrysosporium mycelium | |
| CN103466804B (en) | Aspergillus oryzae cell is utilized to remove anthraquinone analog compound Technology in waste water | |
| Walker et al. | The Language of Biotechnology: A Dictionary of Terms. | |
| CN103466805A (en) | Technique for removing anthraquinone compounds in wastewater by using Ceriporiopsis subvermispora thallus | |
| JP2009261287A (en) | Chlorella/hydrogen production method and chlorella/hydrogen production apparatus | |
| CN103667081B (en) | One strain Lyeopene superior strain and application thereof | |
| CN110029131B (en) | Method for preparing biological pigment by double-bacterium fermentation with amino acid-rich wastewater as base material | |
| CN104277996B (en) | Solve keratan microbacterium and its cultural method and application | |
| Suhaimi et al. | NH+/4‐N assimilation by Rhodobacter capsulatus ATCC 23782 grown axenically and non‐axenically in N and C rich media | |
| CN104342382B (en) | A kind of bacillus and its application in treatment of Phosphorus Containing Waste Water | |
| CN103466808A (en) | Process technology for degrading anthraquinone compound in waste water by employing aspergillus oryzae | |
| CN101418269B (en) | Streptomycete and application thereof in reduction of aromatic ketone |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131225 |