CN114456988B - Functional flora for producing kaempferol and preparation and application thereof - Google Patents

Functional flora for producing kaempferol and preparation and application thereof Download PDF

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CN114456988B
CN114456988B CN202210251057.3A CN202210251057A CN114456988B CN 114456988 B CN114456988 B CN 114456988B CN 202210251057 A CN202210251057 A CN 202210251057A CN 114456988 B CN114456988 B CN 114456988B
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rhizobium
kaempferol
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刘学端
张双飞
梁伊丽
孙崇然
周瑾
张国庆
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Central South University
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Abstract

The invention discloses a functional flora for producing kaempferol, and preparation and application thereof, and belongs to the technical field of producing kaempferol by microbial fermentation. The invention uses potato and glucose as substrates, and utilizes bacillus to change cell membrane permeability and mediation of rhizobia to co-ferment with fusarium to produce kaempferol. The production method is simple, low in production cost, environment-friendly and capable of producing various other flavonoid compounds at high yield. The kaempferol produced by the method has obviously improved yield. The invention solves the problems of serious pollution and high cost in the kaempferol production process, can provide a more natural and sustainable substitute for consumers, promotes the industrialized development of biological fermentation technology, and can realize the large-scale production of kaempferol.

Description

Functional flora for producing kaempferol and preparation and application thereof
Technical Field
The invention relates to a functional flora for producing kaempferol, and preparation and application thereof, and belongs to the technical field of producing kaempferol by microbial fermentation.
Background
Flavonoid compounds are the key points of research and development in the fields of medicines and nutritional products at home and abroad at present. The application direction of the related components of the compound is very wide, and the related components comprise product formulas of skin, inflammation, immunity and the like. From the market data published by Insight SLICE, by 2031, the global flavonoid market share is expected to achieve a 5.5% composite annual growth rate, with a market size of up to $14.5 billion.
Kaempferol is a polyol hydroxyflavone compound widely existing in various plants and has various pharmacological activities such as anticancer, antioxidant, blood pressure reducing, blood lipid reducing and anti-inflammatory reaction. The end products are mainly classified into pharmaceutical grade, food grade and other market segments, wherein the pharmaceutical grade accounts for the highest proportion. According to the published data of the global market research company, 98% of kaempferol market demand in the United states comes from the pharmaceutical industry, and nutritional supplements, functional food beverages, local beauty cream and the like are becoming new development directions. Taking the nutritional supplement industry as an example, kaempferol has great application potential and is mainly used in immune support and inflammation formula products. In summary, kaempferol represents a global consumer market of $ 57 billion from the global market, with great potential.
Currently, the main production process of global kaempferol is mainly chemical synthesis. It is known that most chemically synthesized kaempferol starts with hydroxybenzoic acid as a synthesis precursor. While hydroxybenzoic acid is primarily derived from the chemical reaction of phenol and carbon dioxide, both of which are derived from petroleum and other fossil fuels. With the increasing consumer demand for natural healthy products, it is becoming particularly important how to produce raw materials in a more natural and environmentally friendly process. At the end of 2021, the biosynia and langtana Bio statement of the company danish biotechnology, white biotechnology, france, will create a new technological platform for the development of more sustainable flavonoid production processes by fermentation technology. Biosyntia is responsible for feedstock production and commercialization, the first commercial feedstock being kaempferol. Just before the commercialization of this raw material, the U.S. Conagen company also introduced kaempferol from fermentation technology in the early 2022. Although the whole process avoids using fossil fuel derivatives, the fermentation process is still high in complexity, natural raw materials cannot be directly utilized, specific sugar needs to be extracted from plants first, and then the application cost is high through a series of fermentation processes. But not negligible, biological fermentation is more sustainable than products using fossil products and plant extraction sources. Therefore, by developing and applying a special biological fermentation process, a more natural and sustainable substitute can be provided for consumers, the industrial development of the biological fermentation technology is promoted, and the method has a wide application prospect.
Disclosure of Invention
The primary purpose of the invention is to provide a functional flora for producing kaempferol. The flora takes potato and glucose as substrates, utilizes bacillus to change cell membrane permeability and mediation of rhizobia, builds a co-fermentation process, and can realize remarkable increase of the yield of kaempferol produced by fusarium.
The object of the present invention is achieved in the following manner.
A functional group for producing kaempferol, comprising bacillus belicus (Bacillus velezensis), rhizobium warfarin (Rhizobium mesosinicum) and Fusarium solani.
Further, the functional flora includes: bacillus belicus (Bacillus velezensis) DSM 28326, rhizobium warfarin (Rhizobium mesosinicum) LMG 24135 and Fusarium solani ATCC 36031.
Furthermore, the functional flora is characterized in that two bacteria and fusarium are cultured independently, when the three bacteria reach the logarithmic growth phase, the two bacteria are inoculated into the fermentation liquor of the fusarium according to the inoculum size of 5-10%, and finally, the co-fermentation system is constructed by culturing.
The second object of the present invention is to provide the preparation method of the functional flora, which comprises the steps of culturing bacillus belicus, rhizobium warfarin and fusarium individually, and inoculating bacillus belicus and rhizobium warfarin a fermentation system containing fusarium according to an inoculum size of 5-10% when all the bacillus belicus, rhizobium warfarin and fusarium reach a logarithmic growth phase.
Further, the dry weight of fusarium in the fermentation system reaches 2-3g/100mL, and the fusarium is used for inoculating bacillus belicus and rhizobium warfarin.
According to the preparation method, seed culture mediums of bacillus belicus, rhizobium huwenzhongzhongyuan and fusarium are potato dextrose culture mediums added with 0.1-0.2g/L chloramphenicol. To control the composition of the strain metabolites and colony morphology during fermentation of fusarium. The fusarium fermentation broth does not show dark purple and yellow after chloramphenicol is added, and the fusarium fermentation broth shows dark purple without chloramphenicol, so that the concentration of the bacteria liquid is low and the hyphae are few.
According to the preparation method, bacillus belicus and rhizobium warfarin are respectively inoculated into a culture medium for culture to obtain seed liquid, and the culture conditions are as follows: the temperature is 25-30 ℃, the rotating speed is 160-180rpm, the initial pH value is 6.5-7.5, and the culture time is 1-2 days;
the single culture condition of fusarium at the earlier stage is that the temperature is 25-30 ℃, the rotating speed is 140-160rpm, the initial pH value is 6.5-7.0, and the culture time is 2-4 days.
Before co-fermentation, the seed solution of bacillus belicus and rhizobium warrior needs to be centrifuged to remove supernatant, sterile culture medium is added, after re-suspension, the mixture is centrifuged again, washing is repeated for 3-6 times, after washing is completed, the collected thalli are diluted into bacterial suspension by the sterile culture medium, and then inoculation is carried out.
As a further improvement, before co-fermentation, the seed solution of Bacillus belicus and Rhizobium warfarin is centrifuged at 3000-5000rpm for 8-10 min, the supernatant is removed, an equal amount of sterile culture medium is added, the mixture is resuspended, centrifuged again, washed repeatedly for 3-6 times, and after washing is completed, the collected thallus is diluted to 1X 10 with sterile culture medium 8 -1×10 9 CFU·mL -1 Bacterial suspension, and inoculating.
The third object of the present invention is to provide a method for applying the functional group for producing kaempferol, wherein the functional group is fermented to produce kaempferol, and preferably the co-fermentation culture conditions are as follows: the temperature is 25-30 ℃, the rotating speed is 160-180rpm, the initial pH value is 6.5-8.0, and the culture time is 2-4 days. The fermentation medium is potato glucose medium added with 0.1-0.2g/L chloramphenicol.
In the application method, the strains are required to be strictly shielded in the processes of single culture and mixed fermentation.
As a further improvement, the final fermentation broth is filtered with gauze to separate the cells. 100mL of the obtained filtrate is measured, and the ethyl acetate with the same amount is added for extraction for 3-4 times. The extract is subjected to rotary evaporation for 15 minutes at 60 ℃ by a rotary evaporator, 3mL of chromatographic grade methanol is added for redissolution until no liquid drop is seen at the bottom of a flask, and a 0.22 mu m filter membrane is used for filtering the mixture in a 2mL chromatographic bottle to be detected; filtering with gauze, washing for several times to obtain fungus thallus, baking at 55deg.C for 4-8 hr, grinding, sieving with 400 mesh sieve, extracting flavonoid glycoside with Soxhlet extraction method, and detecting kaempferol content with liquid chromatography. The detection conditions were as follows: the automatic sampler is set to have a sampling volume of 10 mu L and a flow rate of 1.0mL/min; column temperature of the chromatographic column is 25 ℃; the detector is set to be 360nm in detection wavelength; the extraction and elution program is set to 0-20 min, and the mobile phase B:5% -70%; 20-23 min, mobile phase B:70% -5%; and stopping for 25 min. Mobile phase a:0.05mol/L sodium acetate (pH 5.5), mobile phase B:90% acetonitrile.
The invention provides a flora and an application method capable of remarkably improving the kaempferol production of fusarium, which are simple in culture medium components, low in cost and simple and convenient in operation process, can ensure the stability and safety of raw materials, can provide a more natural and sustainable substitute for consumers, promotes the industrial development of biological fermentation technology, and has wide application prospect.
Drawings
FIG. 1 is a high performance liquid chromatography detection spectrum of flavonoids;
FIG. 2 is a graph showing the effect of co-fermentation on kaempferol production;
FIG. 3 shows the effect of chloramphenicol addition or chloramphenicol addition on the fermentation broth of Fusarium.
The left graph is not added, and the right graph is added.
Detailed Description
The present invention is described in detail below by way of specific embodiments, which are only illustrative and not intended to limit the scope of the present invention.
Example 1: functional microorganism primary screening
In order to solve the problems of serious pollution and high cost in the kaempferol production process, a proper strain is firstly required to be selected. According to the basis of the existing researches, fusarium for producing kaempferol is determined as a main fermentation strain. The co-fermented bacterial strain mainly considers that the bacterial strain has the capabilities of hydrolyzing cell walls, changing cell membrane permeability and resisting pressure (adapting to severe fermentation conditions), can antagonize chloramphenicol, can generate a large amount of biological films, can be symbiotic with fusarium, and has vigorous growth and metabolism. The main focus has been on various bacilli and rhizobia. Further, antagonism between strains is reduced, the synergistic effect of the strains is exerted, and bacillus and rhizobia are mainly selected for co-fermentation. The co-fermentation effect of the different combinations is as follows:
the bacillus and rhizobia in the following combinations are inoculated and mixed according to 5% equal proportion, and are cultured and fermented under the same conditions.
Mixing group a: fusarium, bacillus belgium and rhizobium warfarin are subjected to co-fermentation test, and the fermentation liquid measurement result shows that the yield of kaempferol is increased by 189.23-195.46%;
mixing group B: fusarium, bacillus belgium and rhizobium oldhamii are subjected to a co-fermentation test, and the fermentation liquid measurement result shows that the yield of kaempferol is increased by 131.16-145.28%;
mixing group C: fusarium, bacillus pumilus and rhizobium warfarin are subjected to co-fermentation test, and the fermentation liquid measurement result shows that the yield of kaempferol is increased by 22.53-32.41%;
mixing group D: fusarium, bacillus pumilus and rhizobium oldhamii are subjected to a co-fermentation test, and the fermentation liquid measurement result shows that the yield of kaempferol is increased by 30.60-33.16%;
mixing group E: fusarium, bacillus subtilis and rhizobium warfarin are subjected to co-fermentation test, and the fermentation liquid measurement result shows that the yield of kaempferol is increased by 113.34-115.11%;
mixing group F: fusarium, bacillus subtilis and rhizobium oldhamii are subjected to a co-fermentation test, and the fermentation liquid measurement result shows that the yield of kaempferol is increased by 126.23-137.69%;
the above yield increases were relative to the blank control of fusarium fermentation alone, 10 replicates per group, taking minimum and maximum values.
And selecting the mixed group A as a co-fermentation strain for producing the kaempferol according to the results, and optimizing the fermentation process again to finally obtain the controllable fermentation process capable of remarkably improving the yield of the kaempferol.
Example 2: composition and origin of functional microorganisms
The microorganisms involved in the present invention were purchased from respective strain collections, fusarium solani ATCC 36031, bacillus bailii (Bacillus velezensis) DSM 28326, bacillus pumilus ATCC 7061, bacillus subtilis ATCC6051, rhizobium australis LMG 24466 and Rhizobium warfarin LMG 24135.
Strains purchased from the American Type Culture Collection (ATCC) at 5 months 2018 include: fusarium (Fusarium solani) ATCC 36031, bacillus pumilus (Bacillus pumilus) ATCC 7061, and Bacillus subtilis (Bacillus subtilis) ATCC 6051.
Strains purchased from the German collection of microorganisms and cells (DSM) at 10 months 2017 include: bacillus beleimeris (Bacillus velezensis) DSM 28326.
10 months of 2017 purchased from national collection for typical cultures (NTCC) included: rhizobium aureobasidium (Rhizobium alami) LMG 24466 and Rhizobium warfarin (Rhizobium mesosinicum) LMG 24135.
Example 3: method for culturing microorganism strains
Inoculating bacillus bailii and rhizobium warfarin into a culture medium for culture to obtain seed liquid, wherein the culture conditions are as follows: the culture was carried out at 26℃and at 160rpm, with an initial pH of 7.0, in the absence of light until the logarithmic phase. In the whole fermentation process of fusarium, the earlier stage single culture condition is that the temperature is 26 ℃, the rotating speed is 160rpm, the initial pH value is 6.5, and the light-shielding culture time is 3 days; the culture mediums used independently for bacillus belicus, rhizobium huwensis and fusarium are potato dextrose culture mediums added with 0.12g/L chloramphenicol.
Example 4: seed liquid preparation of two functional bacteria
The two functional bacteria cultured in example 3 were centrifuged at 4000rpm for 10 minutes using a high-speed centrifuge, the supernatant was removed, an equal amount of PDB medium was added, and after resuspension, the centrifugation was repeated for 6 times, and after washing was completed, the collected cells were further diluted to 1X 10 with PDB medium in a resuspension manner 9 CFU·mL -1 The bacterial suspensions are inoculated into a fermentation tank of fusarium (the dry weight of the fusarium in a fermentation system reaches 2g per 100mL, and the fusarium is used for inoculating bacillus belicus and rhizobium warfarin) according to the inoculum amount of 5 percent. The co-fermentation culture conditions are as follows: the temperature was 26℃and the rotational speed was 160rpm, the initial pH was 7.0, and the incubation time was 3 days. And carrying out light-shading treatment in the whole fermentation process. The fermentation medium is potato glucose medium added with 0.12g/L chloramphenicol.
Example 5: effect of Co-fermentation method on Kaempferol yield
According to the high performance liquid chromatography detection spectrum of Fusarium flavonoids, it can be found that Fusarium produces kaempferol and simultaneously produces various flavonoids as shown in figure 1 (in figure 1, single-strain fermentation refers to single-strain fermentation of Fusarium). After the fermentation, the final fermentation broth was filtered with gauze to separate the cells. 100mL of the obtained filtrate is measured, and the ethyl acetate with the same amount is added for extraction for 3-4 times. The extract is subjected to rotary evaporation for 15 minutes at 60 ℃ by a rotary evaporator, 3mL of chromatographic grade methanol is added for redissolution until no liquid drop is seen at the bottom of a flask, and a 0.22 mu m organic filter membrane is used for filtering the mixture into 2mL chromatographic bottles to be detected; filtering with gauze, washing for several times to obtain fungus thallus, baking at 55deg.C for 4-8 hr, grinding, sieving with 400 mesh sieve, extracting flavonoid glycoside with Soxhlet extraction method, and detecting kaempferol content with liquid chromatography. The results of repeated experiments show that the yields of kaempferol produced by co-fermentation of the three strains are significantly higher than those of the control group (i.e., fusarium not co-cultured with Bacillus and Rhizobium) in eliminating the possibility of producing kaempferol by the functional bacteria, as shown in FIG. 2. The content of kaempferol obtained by extracting the bacterial liquid can reach 644.49 mug/L, and the content of kaempferol in the bacterial body can reach 25.78 mug/g, so that the yield of kaempferol is improved by at least 200 percent as a whole. Further, the detection of the capacity of producing kaempferol by single bacteria in a mixed mode shows that the difference of the yield of kaempferol is not obvious compared with the yield of the control group whether the fusarium is combined with rhizobium huwenzhou or the fusarium is combined with bacillus bailii (the addition amount of rhizobium huwenzhou or bacillus bailii is equal to the total addition amount of bacillus bailii and rhizobium huzhou). Therefore, the invention is a fermentation process capable of obviously improving the production of kaempferol by fusarium.

Claims (10)

1. A functional flora for producing kaempferol is characterized by comprising bacillus bailiiBacillus velezensis) Rhizobium of ChinaRhizobium mesosinicum) Fusarium solani and its preparationFusarium solani) The method comprises the steps of carrying out a first treatment on the surface of the The bacillus belgium is bacillus belgium DSM 28326, the rhizobium warfarin is rhizobium warfarin LMG 24135, and the fusarium putrescentiae is fusarium putrescentiae ATCC 36031.
2. The functional flora according to claim 1, wherein bacillus belicus, rhizobium warfarin and fusarium putrescens are cultured separately, when all the bacillus belicus, rhizobium warfarin reach logarithmic phase, the bacillus belicus and rhizobium warfarin are inoculated into fermentation liquor of fusarium putrescens according to the inoculum size of 5-10%, and finally the co-fermentation system is built by culture.
3. The method for producing functional bacterial flora according to claim 1 or 2, wherein bacillus belicus, rhizobium warfarin and fusarium putrescens are cultured separately, and when all the three reach the logarithmic growth phase, bacillus belicus and rhizobium warfarin are inoculated into a fermentation system containing fusarium putrescens according to an inoculum size of 5-10%.
4. The method according to claim 3, wherein the dry weight of Fusarium solani in the fermentation system is 2-3g/100mL, and the Fusarium solani is used for inoculating Bacillus belicus and Rhizobium warfarin.
5. The preparation method according to claim 3, wherein the seed culture media of bacillus belicus, rhizobium warfarin and fusarium solani are potato dextrose culture media added with 0.1-0.2g/L chloramphenicol.
6. The method according to claim 3, wherein bacillus belicus and rhizobium huashi are respectively inoculated into a culture medium for culture to obtain seed liquid, and the culture conditions are as follows: the temperature is 25-30 ℃, the rotating speed is 160-180rpm, the initial pH value is 6.5-7.5, and the culture time is 1-2 days; the earlier stage single culture condition of Fusarium solani is that the temperature is 25-30 ℃, the rotating speed is 140-160rpm, the initial pH value is 6.5-7.0, and the culture time is 2-4 days.
7. The method according to claim 3, wherein the seed solution of Bacillus belicus and Rhizobium warfarin is centrifuged to remove supernatant, sterile culture medium is added, re-suspended, centrifuged again, washed repeatedly for 3-6 times, and after washing, the collected thallus is diluted into bacterial suspension with sterile culture medium, and inoculated.
8. The method for using the functional group for producing kaempferol according to claim 1 or 2, wherein the functional group is fermented to produce kaempferol.
9. The method for using a kaempferol-producing functional bacterial population according to claim 8, wherein the fermentation culture conditions are as follows: the temperature is 25-30 ℃, the rotating speed is 160-180rpm, the initial pH value is 6.5-8.0, the culture time is 2-4 days, and the fermentation medium is potato glucose medium added with 0.1-0.2g/L chloramphenicol.
10. The method of claim 8 or 9, wherein the strain is cultivated separately and the whole fermentation process requires stringent shading.
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CN110637082A (en) * 2017-01-04 2019-12-31 诺维信生物农业公司 Bacillus isolate and uses thereof

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* Cited by examiner, † Cited by third party
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CN110637082A (en) * 2017-01-04 2019-12-31 诺维信生物农业公司 Bacillus isolate and uses thereof

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* Cited by examiner, † Cited by third party
Title
Fusarium oxysporum & Fusarium solani: Identification, Characterization, and Differentiation the Fungal Phenolic Profiles by HPLC and the Fungal Lipid Profiles by GC-MS;Nashwa M. Shalapy 等;《Journal of Food Quality》;第1-12页 *
肠道微生物菌群生物转化天然多酚类化合物研究进展;杨艳 等;《食品科学》;第319-325页 *

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