CN103360155A - Preparation method of nematode-proof bio-fertilizer - Google Patents
Preparation method of nematode-proof bio-fertilizer Download PDFInfo
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Abstract
The invention discloses a preparation method of a nematode-proof bio-fertilizer. The preparation method comprises steps of raw material preparation, preparation of microorganism complex microbial inoculants, preparation of fermentation materials and biological fermentation, etc. The preparation method disclosed by the invention has the advantages that feces of beasts and birds, crop straws and mushroom leftovers are fermented to change wastes into valuable materials, thus the pollution caused by the feces to the environment is solved and the soil is improved. The preparation method is simple and low in treatment cost and has a good effect; the nematode eggs and larva can be killed by adding the microorganism complex microbial inoculants, therefore, the fermentation effect and the fertilizer efficiency are improved, a disease prevention effect can be achieved, and the economic efficiency is improved.
Description
Technical field
The present invention relates to a kind of microbial organic fertilizer, specifically a kind of anti-nematode bio-fertilizer preparation method belongs to the biological organic fertilizer field.
Background technology
In recent years, various biological organic fertilizers are generally used in China, and it is reducing fertilizer application, purifies edatope, improve on crops quality and the food safety to have outstanding contribution, and the Sustainable development of agricultural is had great pushing effect.At present, it is raw material that biological organic fertilizer generally adopts stalk or ight soil, and environment protection and agricultural sustainable development are had definite meaning.But most of biological organic fertilizers do not carry out scientific allocation according to practical application, and the fermentation of becoming thoroughly decomposed is nonstandard, and bacterial classification improper use etc. cause and burn root and burn seedling, and fertilizer efficiency, fertilizer efficiency period are suitable with chemically composited fertilizer, and soil improvement such as is not good at the defective.
Along with the developing rapidly of scale animal and poultry cultivation industry, environment has been caused serious pollution in recent years.The year of whole nation feces of livestock and poultry produces total amount about 3,000,000,000 tons, has surpassed the turnout of industrial solid wastes, and cultivation ight soil enters water body flowing water rate up to 25~30%, and in some areas, livestock and poultry breeding industry becomes the source of pollution of local water body maximum just gradually.In addition, the tankage that various farm and sideline product processing industries produce such as the tankage etc. of pestering, have caused waste owing to reasonably not utilizing, and simultaneously environment have also been caused pollution.
Summary of the invention
In order to address the above problem, the present invention has designed a kind of anti-nematode bio-fertilizer preparation method, by to animal dung, just crop material and the mushroom tankage carry out fermentative processing, turn waste into wealth, solved the pollution of ight soil to environment, and soil is improved, the preparation method is simple, effective, processing cost is low, can kill nematode worm's ovum and larva by adding complex microbial inoculum, and improved ferment effect, increase fertilizer efficiency, can also play protection effect, improved economic benefit.
Technical scheme of the present invention is:
A kind of preparation method of anti-nematode bio-fertilizer may further comprise the steps:
(1) raw material is prepared
Component according to following weight part is prepared raw material:
40~60 parts of feces of livestock and poultry, 20~30 parts of tankage of mushroom, 20~30 parts of crop materials, 0.5~1 part of complex microbial inoculum, nitrogenous fertilizer 3-5 part, phosphate fertilizer 4-6 part, potash fertilizer 3-5 part, trace element fertilizer are little fertile 0.2-0.4 part;
The water content of described feces of livestock and poultry is that 40~60%, pH value is 6.5~7.5;
Described crop material is one or more of millet stalk, maize straw, soybean stalk, broomcorn straw, rice straw etc., and carbon-nitrogen ratio is 40~50: 1;
Described complex microbial inoculum is comprised of saccharomyces cerevisiae (Sac-charomyces cerevisiae) bacterium liquid, subtilis (Bacillus subtilis) bacterium liquid, De Shi milk-acid bacteria (L.Delbrueckii) bacterium liquid, A Nandeshi streptomycete (Streptomyces anandii) bacterium liquid, penicillium oxalicum (Penicillium oxalicum) bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacteria liquid are long-pending than being 2~3: 3~4: 3~4: 4~5: 4~8;
Described complex microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation;
To pester tankage and crop material pulverized, and crosses 50~100 mesh sieves;
(2) preparation complex microbial inoculum
Described complex microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required substratum is yeast extract paste 10g, peptone 20g, glucose 20g, distilled water 1000ml; Culture temperature is 28~38 ℃; Aerobic fermentation 24~48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described subtilis bacterium liquid is: required substratum is peptone 15.0g, extractum carnis 0.50g, glucose 20.0g, sodium-chlor 5.0g, distilled water 1000ml; Culture temperature is 30~36 ℃; Aerobic fermentation 48~72h obtains subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required substratum is wort 70mL, extractum carnis 10g, yeast extract paste 5g, soybean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 38~42 ℃; Aerobic fermentation 48~72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required substratum is maltose 1g, glucose 1g, sucrose 1g, Zulkovsky starch 3g, peptone 2g, extractum carnis 10g, yeast extract paste 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 30~35 ℃; Aerobic fermentation 48~72h obtains A Nandeshi streptomycete bacterium liquid;
The preparation method of described penicillium oxalicum bacterium liquid is: required substratum is Semen Maydis powder 3g, soybean cake powder 3g, wheat bran 3g, peanut oil 0.5g, sal epsom 0.1g, potassium primary phosphate 3g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 25~30 ℃; Aerobic fermentation 48~72h obtains penicillium oxalicum bacterium liquid;
According to above-mentioned volume ratio dog's-nose yeast bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacterium liquid, obtain described complex microbial inoculum;
(3) preparation fermentation materials
With feces of livestock and poultry, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, little fertilizer, the mushroom tankage and the crop material that obtain with step (1), and the complex microbial inoculum that obtains of step (2) is equipped with according to above-mentioned weight and mixes;
(4) biological fermentation
The mixed fermentation materials of step (3) is sent into fermentation vat to ferment, the controlled fermentation temperature is at 50~70 ℃, fermentation humidity is 70~80%, stirred once every 1 hour, in the fermenting process foul smell that produces in the fermenter is transported to treatment facility by gas exhaust duct and carries out environmental protection treatment, can obtain anti-nematode bio-fertilizer through 3~5 days.
The invention has the advantages that: by to animal dung, just crop material and the mushroom tankage carry out fermentative processing, turn waste into wealth, solved the pollution of ight soil to environment, and soil is improved, the preparation method is simple, effective, processing cost is low, can kill nematode worm's ovum and larva by adding complex microbial inoculum, and improved ferment effect, increase fertilizer efficiency, can also play protection effect, improved economic benefit.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for description and interpretation the present invention, is not intended to limit the present invention.
Embodiment 1
A kind of anti-nematode bio-fertilizer is grouped into by the one-tenth of following weight part:
Component according to following weight part is prepared raw material:
40~60 parts of feces of livestock and poultry, 20~30 parts of tankage of mushroom, 20~30 parts of crop materials, 0.5~1 part of complex microbial inoculum, nitrogenous fertilizer 3-5 part, phosphate fertilizer 4-6 part, potash fertilizer 3-5 part, trace element fertilizer are little fertile 0.2-0.4 part;
The water content of described feces of livestock and poultry is that 40~60%, pH value is 6.5~7.5;
Described crop material is one or more of millet stalk, maize straw, soybean stalk, broomcorn straw, rice straw etc., and carbon-nitrogen ratio is 40~50: 1;
Described complex microbial inoculum is comprised of saccharomyces cerevisiae bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacteria liquid are long-pending than being 2~3: 3~4: 3~4: 4~5: 4~8;
Described complex microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation.
A kind of preparation method of above-mentioned anti-nematode bio-fertilizer may further comprise the steps:
(1) raw material is prepared
To pester tankage and crop material pulverized, and crosses 50~100 mesh sieves;
(2) preparation complex microbial inoculum
Described complex microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required substratum is yeast extract paste 10g, peptone 20g, glucose 20g, distilled water 1000ml; Culture temperature is 28~38 ℃; Aerobic fermentation 24~48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described subtilis bacterium liquid is: required substratum is peptone 15.0g, extractum carnis 0.50g, glucose 20.0g, sodium-chlor 5.0g, distilled water 1000ml; Culture temperature is 30~36 ℃; Aerobic fermentation 48~72h obtains subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required substratum is wort 70mL, extractum carnis 10g, yeast extract paste 5g, soybean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 38~42 ℃; Aerobic fermentation 48~72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required substratum is maltose 1g, glucose 1g, sucrose 1g, Zulkovsky starch 3g, peptone 2g, extractum carnis 10g, yeast extract paste 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 30~35 ℃; Aerobic fermentation 48~72h obtains A Nandeshi streptomycete bacterium liquid;
The preparation method of described penicillium oxalicum bacterium liquid is: required substratum is Semen Maydis powder 3g, soybean cake powder 3g, wheat bran 3g, peanut oil 0.5g, sal epsom 0.1g, potassium primary phosphate 3g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 25~30 ℃; Aerobic fermentation 48~72h obtains penicillium oxalicum bacterium liquid;
According to above-mentioned volume ratio dog's-nose yeast bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacterium liquid, obtain described complex microbial inoculum;
(3) preparation fermentation materials
With feces of livestock and poultry, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, little fertilizer, the mushroom tankage and the crop material that obtain with step (1), and the complex microbial inoculum that obtains of step (2) is equipped with according to above-mentioned weight and mixes;
(4) biological fermentation
The mixed fermentation materials of step (3) is sent into fermentation vat to ferment, the controlled fermentation temperature is at 50~70 ℃, fermentation humidity is 70~80%, stirred once every 1 hour, in the fermenting process foul smell that produces in the fermenter is transported to treatment facility by gas exhaust duct and carries out environmental protection treatment, can obtain anti-nematode bio-fertilizer through 3~5 days.
Life fertilizer of the present invention is carried out the test of cucumber field production, and control group is set, the result shows:
Test group soil is significantly improved, and the nematode incidence is low by 85% than control group, and almost wireless pests occurs, and the diseases prevention vermins-proof effect is good, output increased 20-30%.
Except as otherwise noted, the percentage ratio that adopts among the present invention is weight percentage.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment the present invention is had been described in detail, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. the preparation method of an anti-nematode bio-fertilizer is characterized in that, may further comprise the steps:
(1) raw material is prepared
Component according to following weight part is prepared raw material:
40~60 parts of feces of livestock and poultry, 20~30 parts of tankage of mushroom, 20~30 parts of crop materials, 0.5~1 part of complex microbial inoculum, nitrogenous fertilizer 3-5 part, phosphate fertilizer 4-6 part, potash fertilizer 3-5 part, trace element fertilizer are little fertile 0.2-0.4 part;
The water content of described feces of livestock and poultry is that 40~60%, pH value is 6.5~7.5;
Described crop material is one or more of millet stalk, maize straw, soybean stalk, broomcorn straw, rice straw etc., and carbon-nitrogen ratio is 40~50: 1;
Described complex microbial inoculum is comprised of saccharomyces cerevisiae bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacteria liquid are long-pending than being 2~3: 3~4: 3~4: 4~5: 4~8;
Described complex microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation;
To pester tankage and crop material pulverized, and crosses 50~100 mesh sieves;
(2) preparation complex microbial inoculum
Described complex microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required substratum is yeast extract paste 10g, peptone 20g, glucose 20g, distilled water 1000ml; Culture temperature is 28~38 ℃; Aerobic fermentation 24~48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described subtilis bacterium liquid is: required substratum is peptone 15.0g, extractum carnis 0.50g, glucose 20.0g, sodium-chlor 5.0g, distilled water 1000ml; Culture temperature is 30~36 ℃; Aerobic fermentation 48~72h obtains subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required substratum is wort 70mL, extractum carnis 10g, yeast extract paste 5g, soybean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 38~42 ℃; Aerobic fermentation 48~72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required substratum is maltose 1g, glucose 1g, sucrose 1g, Zulkovsky starch 3g, peptone 2g, extractum carnis 10g, yeast extract paste 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 30~35 ℃; Aerobic fermentation 48~72h obtains A Nandeshi streptomycete bacterium liquid;
The preparation method of described penicillium oxalicum bacterium liquid is: required substratum is Semen Maydis powder 3g, soybean cake powder 3g, wheat bran 3g, peanut oil 0.5g, sal epsom 0.1g, potassium primary phosphate 3g, add water to 1000ml, initial pH value is 6.5; Culture temperature is 25~30 ℃; Aerobic fermentation 48~72h obtains penicillium oxalicum bacterium liquid;
According to above-mentioned volume ratio dog's-nose yeast bacterium liquid, subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, penicillium oxalicum bacterium liquid, obtain described complex microbial inoculum;
(3) preparation fermentation materials
With feces of livestock and poultry, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, little fertilizer, the mushroom tankage and the crop material that obtain with step (1), and the complex microbial inoculum that obtains of step (2) is equipped with according to above-mentioned weight and mixes;
(4) biological fermentation
The mixed fermentation materials of step (3) is sent into fermentation vat to ferment, the controlled fermentation temperature is at 50~70 ℃, fermentation humidity is 70~80%, stirred once every 1 hour, in the fermenting process foul smell that produces in the fermenter is transported to treatment facility by gas exhaust duct and carries out environmental protection treatment, can obtain anti-nematode bio-fertilizer through 3~5 days.
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CN104355706A (en) * | 2014-11-15 | 2015-02-18 | 云南农业大学 | Oyster mushroom residue organic fertilizer and application method thereof |
CN104909867A (en) * | 2014-03-12 | 2015-09-16 | 随州新欣农业有限公司 | Bioorganic fertilizer and preparation method thereof |
CN104973913A (en) * | 2014-04-04 | 2015-10-14 | 随州新欣农业有限公司 | Bio-organic fertilizer containing mannose peptides and preparation method of bio-organic fertilizer |
CN105347926A (en) * | 2015-11-10 | 2016-02-24 | 绥化市凯华农业科技有限公司 | Preparation method for microbial organic soil bactericidal and insecticidal warming conditioning agent |
CN106187511A (en) * | 2016-07-20 | 2016-12-07 | 山东爱福地生物科技有限公司 | A kind of microbial manure preventing and treating root-knot nematode and preparation method thereof |
CN106336320A (en) * | 2016-08-16 | 2017-01-18 | 朱兰英 | Preparation method of fertilizer for improving harmful phytophagous nematode in soil |
CN107285953A (en) * | 2017-06-29 | 2017-10-24 | 重庆市万源禽蛋食品有限公司 | Composite zymocyte, biological organic fertilizer and preparation method thereof |
CN112573970A (en) * | 2020-11-05 | 2021-03-30 | 东北农业大学 | Solid-state compound microbial foliar fertilizer for improving saline-alkali soil and application thereof |
CN115226545A (en) * | 2022-08-05 | 2022-10-25 | 江苏省农业科学院泰州农科所 | Method for preventing and treating root knot nematode disease of solanaceous melon and fruit vegetables |
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CN106187511A (en) * | 2016-07-20 | 2016-12-07 | 山东爱福地生物科技有限公司 | A kind of microbial manure preventing and treating root-knot nematode and preparation method thereof |
CN106336320A (en) * | 2016-08-16 | 2017-01-18 | 朱兰英 | Preparation method of fertilizer for improving harmful phytophagous nematode in soil |
CN107285953A (en) * | 2017-06-29 | 2017-10-24 | 重庆市万源禽蛋食品有限公司 | Composite zymocyte, biological organic fertilizer and preparation method thereof |
CN112573970A (en) * | 2020-11-05 | 2021-03-30 | 东北农业大学 | Solid-state compound microbial foliar fertilizer for improving saline-alkali soil and application thereof |
CN115226545A (en) * | 2022-08-05 | 2022-10-25 | 江苏省农业科学院泰州农科所 | Method for preventing and treating root knot nematode disease of solanaceous melon and fruit vegetables |
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Application publication date: 20131023 |