CN103289978B - 基因重组毕赤氏酵母高表达发酵生产舍雷肽酶的方法 - Google Patents
基因重组毕赤氏酵母高表达发酵生产舍雷肽酶的方法 Download PDFInfo
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- CN103289978B CN103289978B CN201310192320.7A CN201310192320A CN103289978B CN 103289978 B CN103289978 B CN 103289978B CN 201310192320 A CN201310192320 A CN 201310192320A CN 103289978 B CN103289978 B CN 103289978B
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- Prior art keywords
- serratiopeptidase
- gene
- pichia pastoris
- serrapeptase
- high expression
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- 108010038132 serratiopeptidase Proteins 0.000 title claims abstract description 45
- 241000235058 Komagataella pastoris Species 0.000 title claims abstract description 20
- 238000000855 fermentation Methods 0.000 title abstract description 6
- 230000004151 fermentation Effects 0.000 title abstract description 6
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- POOYFSLWYLDQMD-UHFFFAOYSA-N heptacalcium;zinc Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Zn+2] POOYFSLWYLDQMD-UHFFFAOYSA-N 0.000 title abstract 6
- 229950000112 serrapeptase Drugs 0.000 title abstract 6
- 230000002068 genetic effect Effects 0.000 title abstract 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 39
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 16
- 230000006698 induction Effects 0.000 claims abstract description 10
- 239000013612 plasmid Substances 0.000 claims abstract description 10
- 238000005516 engineering process Methods 0.000 claims abstract description 9
- 102000018120 Recombinases Human genes 0.000 claims abstract description 7
- 108010091086 Recombinases Proteins 0.000 claims abstract description 7
- 229940000634 serratiopeptidase Drugs 0.000 claims description 37
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 241000235648 Pichia Species 0.000 claims description 12
- 238000005119 centrifugation Methods 0.000 claims description 9
- 238000012262 fermentative production Methods 0.000 claims description 9
- 238000005215 recombination Methods 0.000 claims description 9
- 230000006798 recombination Effects 0.000 claims description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 229930003756 Vitamin B7 Natural products 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000011735 vitamin B7 Substances 0.000 claims description 6
- 235000011912 vitamin B7 Nutrition 0.000 claims description 6
- 241000607720 Serratia Species 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 229940079593 drug Drugs 0.000 abstract 2
- 239000003814 drug Substances 0.000 abstract 2
- 101150017038 Ser gene Proteins 0.000 abstract 1
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- 238000012215 gene cloning Methods 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 208000026435 phlegm Diseases 0.000 description 3
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 206010061926 Purulence Diseases 0.000 description 2
- 102000018594 Tumour necrosis factor Human genes 0.000 description 2
- 108050007852 Tumour necrosis factor Proteins 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 101800004538 Bradykinin Proteins 0.000 description 1
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- QXZGBUJJYSLZLT-UHFFFAOYSA-N H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH Natural products NC(N)=NCCCC(N)C(=O)N1CCCC1C(=O)N1C(C(=O)NCC(=O)NC(CC=2C=CC=CC=2)C(=O)NC(CO)C(=O)N2C(CCC2)C(=O)NC(CC=2C=CC=CC=2)C(=O)NC(CCCN=C(N)N)C(O)=O)CCC1 QXZGBUJJYSLZLT-UHFFFAOYSA-N 0.000 description 1
- 102100035792 Kininogen-1 Human genes 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 208000018569 Respiratory Tract disease Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- QXZGBUJJYSLZLT-FDISYFBBSA-N bradykinin Chemical compound NC(=N)NCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(=O)NCC(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CO)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)CCC1 QXZGBUJJYSLZLT-FDISYFBBSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
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- 239000000047 product Substances 0.000 description 1
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- 230000008961 swelling Effects 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
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CN201310192320.7A CN103289978B (zh) | 2013-05-21 | 2013-05-21 | 基因重组毕赤氏酵母高表达发酵生产舍雷肽酶的方法 |
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CN201310192320.7A CN103289978B (zh) | 2013-05-21 | 2013-05-21 | 基因重组毕赤氏酵母高表达发酵生产舍雷肽酶的方法 |
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CN103289978A CN103289978A (zh) | 2013-09-11 |
CN103289978B true CN103289978B (zh) | 2015-06-17 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN86107809A (zh) * | 1985-11-15 | 1987-07-15 | 武田药品工业株式会社 | 新的重组基因及其应用 |
CN102416172A (zh) * | 2011-12-07 | 2012-04-18 | 北京阜康仁生物制药科技有限公司 | 一种通过吸入给药的舍雷肽酶制剂 |
-
2013
- 2013-05-21 CN CN201310192320.7A patent/CN103289978B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN86107809A (zh) * | 1985-11-15 | 1987-07-15 | 武田药品工业株式会社 | 新的重组基因及其应用 |
CN102416172A (zh) * | 2011-12-07 | 2012-04-18 | 北京阜康仁生物制药科技有限公司 | 一种通过吸入给药的舍雷肽酶制剂 |
Non-Patent Citations (1)
Title |
---|
舍雷肽酶的药理作用及临床评价;潘玉等;《中国新药与临床杂志》;20080831;第27卷(第8期);604-608 * |
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Owner name: NINGBO XINUOYA MARINE BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: NINGBO NEWGEN BIOTECHNOLOGY CO., LTD. Effective date: 20150518 |
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Effective date of registration: 20150518 Address after: 315706 Zhejiang Province, Ningbo city Xiangshan County East Wing Industrial Park Road No. 5 Applicant after: NINGBO XINUOYA MARINE BIOTECHNOLOGY Co.,Ltd. Address before: 315100 Zhejiang city of Ningbo province Yinzhou District song Xiao Gao Road No. 199 South Street Applicant before: NINGBO NEWGEN BIOTECH Co.,Ltd. |
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Denomination of invention: High expression fermentation of recombinant Pichia pastoris to produce seretidase Effective date of registration: 20210927 Granted publication date: 20150617 Pledgee: Ningbo Tianjin Enterprise Service Co.,Ltd. Pledgor: NINGBO XINUOYA MARINE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021330001782 |
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Date of cancellation: 20220926 Granted publication date: 20150617 Pledgee: Ningbo Tianjin Enterprise Service Co.,Ltd. Pledgor: NINGBO XINUOYA MARINE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021330001782 |