CN103288644A - Preparation method for extracting chlorogenic acid from honeysuckle - Google Patents
Preparation method for extracting chlorogenic acid from honeysuckle Download PDFInfo
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- CN103288644A CN103288644A CN 201310179170 CN201310179170A CN103288644A CN 103288644 A CN103288644 A CN 103288644A CN 201310179170 CN201310179170 CN 201310179170 CN 201310179170 A CN201310179170 A CN 201310179170A CN 103288644 A CN103288644 A CN 103288644A
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- honeysuckle
- chlorogenic acid
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- drying
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Abstract
The invention discloses a preparation method for extracting chlorogenic acid from honeysuckle. The preparation method is characterized by comprising the following steps of: a, preparing a raw medicinal material, namely the honeysuckle, cleaning and drying the honeysuckle, and smashing the honeysuckle into honeysuckle powder of 10-30 meshes; b, extracting the honeysuckle powder obtained in the step a for 2-5 times with each extraction time for 1-3 hours, and combining the extracts; c, adjusting the pH value of the extract obtained in the step b to 2-6, then adsorbing the extract by macroporous resin, diluting the extract by ethanol of 70-80 percent and 50-60 percent, and combining diluted liquids to obtain a rough extracted object; and d, adjusting the pH value of the rough extracted object obtained in the step c, then extracting, separating, concentrating and drying the rough extracted object to obtain the finished product chlorogenic acid. The preparation method has the advantages that the technology is simple, the extraction temperature is low, effective components cannot be damaged, the extracted chlorogenic acid content is high, and the treating effect is better.
Description
Technical field
The present invention relates to the gold and silver floral essence, relate in particular to a kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting.
Background technology
Japanese Honeysuckle is the dry flower of caprifoliaceae plant honeysuckle, lonicera hypoglauca miq, Lonicera confusa DC. or the flower that band is just opened.Japanese Honeysuckle is conventional Chinese medicine, and is cold in nature, and it is sweet to distinguish the flavor of, and has significantly clearing heat and detoxicating, antisepsis and anti-inflammation and antivirus action, and wind-heat dissipating is used for that carbuncle is swollen treats sore, larynx numbness, erysipelas, warm blood fulminant dysentery, common cold due to wind-heat, warm disease heating.One of the effective constituent that mainly contains wherein is chlorogenic acid, and chlorogenic acid is considered to the main effective constituent of numerous medicinal materials and Chinese patent medicine antibacterial and detoxicating, anti-inflammatory and choleretic, is used as qualitative even quantitative index usually.Processing method step complexity of the prior art, it is higher to extract temperature, causes effective constituent destroyed, and it is low to extract the products therefrom chlorogenic acid content, and curative effect has much room for improvement.
Therefore, be badly in need of a kind of improved technology and solve existing this problem in the prior art.
Summary of the invention
The purpose of this invention is to provide a kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting.
The technical solution used in the present invention is:
A kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting, may further comprise the steps:
A: prepare the crude drug Japanese Honeysuckle, clean, drying is crushed to the 10-30 order, gets Honeysuckle Flower;
B: the Honeysuckle Flower of gained among the step a is extracted 2-5 time each 1-3h, united extraction liquid in water;
C: the pH value of the extracting solution of gained is 2-6 among the set-up procedure b, uses macroporous resin adsorption then, with the ethanol of 70-80%, the ethanol difference wash-out of 50-60%, merges elutriant again, gets crude extract;
D: the pH value of the crude extract of set-up procedure c gained is 2-6, and extraction then separates, and concentrates, and drying gets the finished product chlorogenic acid.
Temperature dry among the described step a is 50-60 ℃, and be 1-2h time of drying.
The mass ratio of Honeysuckle Flower and water is 1:10-20 among the described step b.
Advantage of the present invention is: the inventive method technology is simple, and it is low to extract temperature, and effective constituent can be not destroyed, extracts products therefrom chlorogenic acid content height, and curative effect is also better.
Embodiment
Embodiment 1
Of the present invention a kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting, may further comprise the steps:
A: prepare the crude drug Japanese Honeysuckle, clean, drying, dry temperature is 50 ℃, be 2h time of drying, is crushed to 10 orders, gets Honeysuckle Flower;
B: the Honeysuckle Flower of gained among the step a is extracted in water 2 times, each 3h, the mass ratio of Honeysuckle Flower and water is 1:10, united extraction liquid;
C: the pH value of the extracting solution of gained is 2 among the set-up procedure b, uses macroporous resin adsorption then, with 70% ethanol, 50% ethanol difference wash-out, merges elutriant again, gets crude extract;
D: the pH value of the crude extract of set-up procedure c gained is 2, and extraction then separates, and concentrates, and drying gets the finished product chlorogenic acid.
The inventive method technology is simple, and it is low to extract temperature, and effective constituent can be not destroyed, extracts products therefrom chlorogenic acid content height, and curative effect is also better.
Embodiment 2
Of the present invention a kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting, may further comprise the steps:
A: prepare the crude drug Japanese Honeysuckle, clean, drying, dry temperature is 55 ℃, be 1.5h time of drying, is crushed to 20 orders, gets Honeysuckle Flower;
B: the Honeysuckle Flower of gained among the step a is extracted in water 3 times, each 2h, the mass ratio of Honeysuckle Flower and water is 1:15, united extraction liquid;
C: the pH value of the extracting solution of gained is 4 among the set-up procedure b, uses macroporous resin adsorption then, with 75% ethanol, 55% ethanol difference wash-out, merges elutriant again, gets crude extract;
D: the pH value of the crude extract of set-up procedure c gained is 4, and extraction then separates, and concentrates, and drying gets the finished product chlorogenic acid.
The inventive method technology is simple, and it is low to extract temperature, and effective constituent can be not destroyed, extracts products therefrom chlorogenic acid content height, and curative effect is also better.
Embodiment 3
Of the present invention a kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting, may further comprise the steps:
A: prepare the crude drug Japanese Honeysuckle, clean, drying, dry temperature is 60 ℃, be 1h time of drying, is crushed to 30 orders, gets Honeysuckle Flower;
B: the Honeysuckle Flower of gained among the step a is extracted in water 5 times, each 1h, the mass ratio of Honeysuckle Flower and water is 1:20, united extraction liquid;
C: the pH value of the extracting solution of gained is 6 among the set-up procedure b, uses macroporous resin adsorption then, with 80% ethanol, 60% ethanol difference wash-out, merges elutriant again, gets crude extract;
D: the pH value of the crude extract of set-up procedure c gained is 6, and extraction then separates, and concentrates, and drying gets the finished product chlorogenic acid.
The inventive method technology is simple, and it is low to extract temperature, and effective constituent can be not destroyed, extracts products therefrom chlorogenic acid content height, and curative effect is also better.
Claims (3)
1. the preparation method of a chlorogenic acid extracting from Japanese Honeysuckle is characterized in that: may further comprise the steps:
A: prepare the crude drug Japanese Honeysuckle, clean, drying is crushed to the 10-30 order, gets Honeysuckle Flower;
B: the Honeysuckle Flower of gained among the step a is extracted 2-5 time each 1-3h, united extraction liquid in water;
C: the pH value of the extracting solution of gained is 2-6 among the set-up procedure b, uses macroporous resin adsorption then, with the ethanol of 70-80%, the ethanol difference wash-out of 50-60%, merges elutriant again, gets crude extract;
D: the pH value of the crude extract of set-up procedure c gained is 2-6, and extraction then separates, and concentrates, and drying gets the finished product chlorogenic acid.
According to claim 1 a kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting, it is characterized in that: temperature dry among the described step a is 50-60 ℃, and be 1-2h time of drying.
According to claim 1 a kind of from Japanese Honeysuckle the preparation method of chlorogenic acid extracting, it is characterized in that: the mass ratio of Honeysuckle Flower and water is 1:10-20 among the described step b.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310179170 CN103288644A (en) | 2013-05-15 | 2013-05-15 | Preparation method for extracting chlorogenic acid from honeysuckle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310179170 CN103288644A (en) | 2013-05-15 | 2013-05-15 | Preparation method for extracting chlorogenic acid from honeysuckle |
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| CN103288644A true CN103288644A (en) | 2013-09-11 |
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| CN 201310179170 Pending CN103288644A (en) | 2013-05-15 | 2013-05-15 | Preparation method for extracting chlorogenic acid from honeysuckle |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375393A (en) * | 2017-07-14 | 2017-11-24 | 临沂大学 | A kind of honeysuckle organic acid constituents coating of pellets particle and preparation method thereof |
-
2013
- 2013-05-15 CN CN 201310179170 patent/CN103288644A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375393A (en) * | 2017-07-14 | 2017-11-24 | 临沂大学 | A kind of honeysuckle organic acid constituents coating of pellets particle and preparation method thereof |
| CN107375393B (en) * | 2017-07-14 | 2021-05-07 | 临沂大学 | Honeysuckle organic acid component pellet coating granule and preparation method thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130911 |