CN103271983A - Perfusate for treating endometritis and kysthitis of pig as well as preparation method and application thereof - Google Patents
Perfusate for treating endometritis and kysthitis of pig as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention provides perfusate for treating endometritis and kysthitis of a pig. The effective ingredient is total alkaloid of sophora alopecuroides. The invention also provides a preparation method and application of the perfusate. The perfusate disclosed by the invention has the advantages of antibiosis, antiphlogosis, low toxicity, no residue, no easily generated drug resistance and the like, and has small thrill and low cost; the diseases such as endometritis and kysthitis of the pig caused by bacteria, fungus and trichomonad can be effectively treated; and especially targeted therapy of the endometritis and the kysthitis of the pig is carried out. The extract with a single traditional Chinese medicine benefits for the stability and the quality controllability of the product; absorption of a digestive tract is reduced by release of a targeting preparation in endometrium and vagina of the pig; the extract can be directly released in the lesion, and can be dispersed into the endometritis and the kysthitis of the pig at high concentration, and is more effective and accurate in treatment of the diseases; the targeting preparation reduces the thrill of the drug on the upper digestive tract, is less in bad reaction, convenient to use, and good in compliance; and good economic benefit is obtained by cultivation enterprises of pigs.
Description
Technical field
The present invention relates to a kind of medicinal liquid, particularly, relate to a kind of endometritis and colpitic infusion liquid for the treatment of pig.
Background technology
The vaginitis of pig, endometritis are a kind of common Main Reproductive Organs disease in Swine Production, most sick pigs are external intrusion pathogen and infecting, as injury of birth canal is polluted when giving a birth, the afterbirth fragment is remaining, lochia is detained during atony of uterus, the artificial insemination sterilization is not tight, and rheuminess thing etc. is arranged in boar genitals or the seminal fluid during natural mating.How per vaginam scorching and then develop into endometritis its cause of disease has diplococcus, streptococcus agalactiae, staphylococcus aureus and escherichia coli.According to clinical statistics, the undesired or delay of oestrus of sow, joining infertile sow repeatly is to suffer from endometritis more than 80%, understands provisions sow family like this and brings heavy economic loss.This disease is sickness rate height, difficult control clinically, causes enormous economic loss for the aquaculture of pig.
Endometritis, the vaginitis prophylactico-therapeutic measures of pig at present is a lot, and as anti-inflammation, enhancing body resistance, and with vaccine immunity and good management raising etc., but the endometritis of pig, vaginitis incidence probability are still high especially.Particularly endometritis, the vaginitis of the clinical antibiotic that generally adopts and chemical medicine treatment pig at first are the endometritis of pig, colpitic clinical symptom disappearance, but are easy to recurrence; Secondly antibiotic and the antibiotic drug resistance ubiquity of chemical medicine use the dosage of antibiotic and chemical medicine continuing to increase, but clinical efficacy are bad, adds antibiotic remains, directly harm humans health.
Herba Sophorae alopecuroidis is perennial leguminous herbaceous plant, dry herb and the seed of Herba Sophorae alopecuroidis.Herba Sophorae alopecuroidis is distributed widely in ground such as Gansu, Ningxia, Xinjiang, Tibet, the Inner Mongol.Herb is gathered summer, and seed is gathered spring, drying.Herb is given birth to usefulness, and seed is fried and used.Country lists Herba Sophorae alopecuroidis in one of six big authentic medicinal herbses of focused protection in recent years, includes in the national modernization of Chinese medicine scientific and technological industry action plan, for construction of production base and deep level development significant progress is arranged.
The Herba Sophorae alopecuroidis flavor is extremely bitter, cold in nature poisonous, has heat-clearing and toxic substances removing, effects such as anti-inflammation, pain relieving parasite killing.Among the people with radical cure treatment laryngalgia, cough, dysentery and eczema etc.The 8 kinds of alkaloids that mainly contain that are used for drug development at present: sophocarpine, matrine, oxymatrine, N-oxysophocarpine one, sophoramine, sophoridine, lehmannine, aloperine.
The pharmacological action of Sophora alopecuroides alkali: 1, antibacterial action, successfully developed treatment dysentery medicine Herba Sophorae alopecuroidis sheet and treatment gynecological inflammation medicine anti-inflammatory suppository for gynecopathy (Herba Sophorae alopecuroidis) bolt at present; 2, to the effect of cardiovascular system, be embodied in arrhythmia, positive inotropic action, blood pressure lowering and reduction heart rate function are to the protective effect of myocardial ischemia and infraction, effect for reducing blood fat; 3, antiviral, antiinflammatory and immunosuppressive action, Herba Sophorae alopecuroidis total alkali can suppress multiple virus breeding. enhance immunity regulatory function and immunologic enhancement; 4, antitumor action, obvious to the hepatoma carcinoma cell inhibitory action, cervical cancer, lymphosarcoma, pulmonary carcinoma also there are inhibitory action; 5, to the effect of smooth muscle, Herba Sophorae alopecuroidis total alkali and some monosomic alkali all can resist the stripped ileum of being induced by inflammatory mediator and shrink, bronchial smooth muscle had relexation, block mold is also had effect: 6, clinical practice Herba Sophorae alopecuroidis extractum sheet, aloperine are treated acute bacillary dysentery at present, sophocarpine sheet treatment bronchial asthma, matrine treatment gynecological inflammation, Treated by Oxymatrine Injection chronic viral hepatitis B are all obtained better curative effect.
Summary of the invention
The invention provides a kind of endometritis and colpitic infusion liquid for the treatment of pig, is to be raw material with the Herba Sophorae alopecuroidis total alkali, has good therapeutic effect, and is difficult for recurrence; Because what use is the pure natural plants medicine, so also there is not drug-fast problem.The present invention also provides the preparation method and application of this infusion liquid.
The invention provides a kind of endometritis and colpitic infusion liquid for the treatment of pig, the effective ingredient of described infusion liquid is Herba Sophorae alopecuroidis total alkali.
Preferably, described infusion liquid contains the Herba Sophorae alopecuroidis total alkali of mass percent 3-8%.
Preferably, described infusion liquid is prepared from by following steps:
1) preparation Herba Sophorae alopecuroidis total alkali: get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, the adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W filters the back and reclaims ethanol, and adding 2% hydrochloric acid accent pH is 2-5, add ether washing 2 times, obtain the filtrate precipitation, add three precipitations of chloroform extraction again, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) preparation infusion liquid: in the Herba Sophorae alopecuroidis total alkali that step 1) obtains, add the water for injection standardize solution, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, under aseptic condition, carry out fill and obtain infusion liquid.
The present invention also provides a kind of endometritis of pig and preparation method of colpitic infusion liquid for the treatment of, and step is as follows:
1) preparation Herba Sophorae alopecuroidis total alkali: get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, the adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W filters the back and reclaims ethanol, and adding 2% hydrochloric acid accent pH is 2-5, add ether washing 2 times, obtain the filtrate precipitation, add three precipitations of chloroform extraction again, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) preparation infusion liquid: in the Herba Sophorae alopecuroidis total alkali that step 1) obtains, add the water for injection standardize solution, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, under aseptic condition, carry out fill and obtain infusion liquid.
Wherein, Herba Sophorae alopecuroidis total alkali can be selected commercially available product.
The 3rd purpose of the present invention provides a kind of preparation method of Herba Sophorae alopecuroidis total alkali, step is as follows: get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, the adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W, filter the back and reclaim ethanol, adding 2% hydrochloric acid accent pH is 2-5, adds ether washing 2 times, obtains the filtrate precipitation, add three precipitations of chloroform extraction again, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali.
The 4th purpose of the present invention provides Herba Sophorae alopecuroidis total alkali in the endometritis for the treatment of pig and the application in the vaginitis.
The 5th purpose of the present invention provides the Herba Sophorae alopecuroidis total alkali infusion liquid in the endometritis for the treatment of pig and the application in the vaginitis.
Preferably, the quality percentage composition of Herba Sophorae alopecuroidis total alkali is 3-8% in the described Herba Sophorae alopecuroidis total alkali infusion liquid.
Preferably, be that the Herba Sophorae alopecuroidis total alkali infusion liquid is filled in the uterus of pig, 100 milliliters of each consumptions, once a day.
That infusion liquid of the present invention has is antibiotic, antiinflammatory, low toxicity, noresidue, be difficult for producing advantage such as drug resistance, and zest is little, cost is lower, can effectively treat the disease, the particularly endometritis of targeted therapy pig, vaginitis such as endometritis, vaginitis of the pig that causes because of antibacterial, fungus, infusorian.The present invention has the extract of single medicinal material, is conducive to stability and the quality controllability of product; Targeting preparation discharges in the endometrium of pig and intravaginal, reduces gastral absorption, directly discharges and can higher concentration be scattered in endometrium and the intravaginal of pig at diseased region, and is more effective, definite to treatment of diseases; Targeting preparation reduces medicine to upper gastrointestinal stimulation, and untoward reaction is few, and is easy to use, and compliance is good.Make the breeding enterprise of pig obtain good economic benefit.
The specific embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment if no special instructions, is conventional method.The Herba Sophorae alopecuroidis total alkali that uses among the following embodiment also can be selected commercially availablely, and its effect is identical with the homemade Herba Sophorae alopecuroidis total alkali of the applicant.
Embodiment one Oxford agar diffusion method is measured the antibacterial activity in vitro of Herba Sophorae alopecuroidis total alkali
Adopt the Oxford agar diffusion method to measure Herba Sophorae alopecuroidis total alkali to staphylococcus aureus, escherichia coli, streptococcus agalactiae, diplococcal antibacterial activity.Result of the test shows that when Herba Sophorae alopecuroidis total alkali concentration was 5mg/ml, staphylococcus aureus, streptococcus agalactiae, diplococcus, colibacillary antibacterial circle diameter were respectively 10.05mm, 6.89mm, 10.66mm, 9.94mm.Antibacterial circle diameter is respectively 13.98mm, 12.93mm, 19.61mm, 19.30mm when 20mg/ml.As can be seen along with the rising of concentration, Herba Sophorae alopecuroidis total alkali to escherichia coli and diplococcal inhibitory action apparently higher than to staphylococcus aureus and streptococcus agalactiae.Wherein the strongest to diplococcal inhibitory action, the most weak to relative other the three kinds of bacterium of the inhibitory action of streptococcus agalactiae.
1 materials and methods
1.1 material and reagent
For trying bacterium: staphylococcus aureus, escherichia coli, streptococcus agalactiae, diplococcus provide by Lanzhou herding and veterinary drug institute veterinary drug research department.
For sample: Herba Sophorae alopecuroidis total alkali, produced by Hinapharm Pharmaceutical Co., Ltd., identical among preparation method and the embodiment two, be mixed with the test liquid of variable concentrations after dissolving with water for injection.
1.2 instrument and equipment
KH-300DB type numerical control supersonic washer, Kunshan standing grain wound ultrasonic instrument company limited;
The RE-201D Rotary Evaporators, Yuhua Instrument Co., Ltd., Gongyi City;
Electro-heating standing-temperature cultivator, Shanghai letter be electronics technology company limited DNP-9272A type all;
Constant temperature oscillator, the U.S. smart black horse company limited IS-RDV1;
Biohazard Safety Equipment, Shen, Shanghai power scientific instrument company limited HFsafe-1500.
1.3 the preparation of bacteria suspension
The bacterium liquid that activation culture is good inserts respectively in the physiological saline solution, carries out gradient dilution successively, and making the bacterial population in the bacteria suspension is 10
7Cfu/ml.
1.4 the Oxford agar diffusion method is measured antibacterial activity
The culture medium 20ml that melted is added in the culture dish that autoclaving crosses, solidify the back and draw the adding of 200 microlitre bacteria suspensions wherein, with spreader with the coating of bacterium liquid evenly, make the plate that carries disease germs, Oxford cup with aseptic nipper gripping high pressure steam sterilization and oven dry, be flat on the culture dish surface that has been coated with bacterium liquid gently, each culture dish cross symmetry is placed 4 Oxford cups, pipette the confession sample of 200 microlitre different quality concentration respectively in the cup of Oxford with aseptic liquid-transfering gun, culture dish is slowly moved into constant incubator, cultivate 18h for 37 ℃, take out each culture dish colony growth situation of observation, and measure antibacterial circle diameter with slide gauge, record data are respectively 5mg/ml for the concentration of sample, 10mg/ml, 15mg/ml, 20mg/ml.
2 results
Measure result such as following table 1 by above method.
3 conclusions
Herba Sophorae alopecuroidis total alkali all has inhibitory action to the endometritis of pig, colpitic main several pathogenic bacterium, and the rising inhibitory action along with Herba Sophorae alopecuroidis total alkali concentration also progressively strengthens in certain scope, wherein the strongest to diplococcal inhibitory action, the most weak to relative other the three kinds of bacterium of the inhibitory action of streptococcus agalactiae.Can further study Herba Sophorae alopecuroidis total alkali to the inhibitory action of other antibacterial, for the development and use of Herba Sophorae alopecuroidis total alkali provide reference frame.
Embodiment two: Herba Sophorae alopecuroidis total alkali is studied acute toxicity test in mice
Adopt the improvement karber's method to measure Herba Sophorae alopecuroidis total alkali to the acute toxicity of mouse peritoneal injection, estimate its safety, for data for clinical drug use provides foundation.During test, give the Herba Sophorae alopecuroidis total alkali of test group mouse gavaging variable concentrations, activity and the death condition of mice after the observation administration adopt improvement Kou Shi formula to calculate LD
50And LD
5095% fiducial limit, the result shows that Herba Sophorae alopecuroidis total alkali is through the LD of lumbar injection
50Be 559.24mg/kg, LD
50The 95% credible 471.95mg/kg～663.59ma/kg that is limited to.Result of the test is tentatively pointed out, and the toxicity of Herba Sophorae alopecuroidis total alkali is lower, and data for clinical drug use is reliable.
1 materials and methods
1.1 material
1.1.1 experimental animal
Kunming system cleaning level healthy mice, male and female half and half, body weight (20 ± 2) g is available from Lanzhou University experimental animal center (sweet doctor moves word 14-005).
1.1.2 be subjected to the reagent thing
Herba Sophorae alopecuroidis total alkali extracts preparation by Hinapharm Pharmaceutical Co., Ltd..
1.2 method
1.2.1 the extraction of Herba Sophorae alopecuroidis total alkali
Get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, the adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W, filter the back and reclaim ethanol, adding 2% hydrochloric acid accent pH is 2-5, adds ether washing 2 times, obtain the filtrate precipitation, add three precipitations of chloroform extraction again, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali, after testing, the purity of Herba Sophorae alopecuroidis total alkali is 98%, and detecting instrument is that the high-efficient liquid phase color spectrometer detects.
Detection method is as follows: 6% Herba Sophorae alopecuroidis total alkali infusion liquid (in Herba Sophorae alopecuroidis total alkali); Sophoridine reference substance (lot number: 11060901, content 〉=98%), sophocarpine reference substance (lot number: 11052422, content 〉=98%) available from Chengdu Man Site bio tech ltd; Matrine reference substance (lot number: 110805-200508) available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute; Acetonitrile is chromatographically pure, and available from Shandong chemical industry company limited in pairs, other reagent are analytical pure.Chromatographic column: Y M C-p a c k e d O D S-A post (1 50 m m * 4. 6 m m, 5 μ m), mobile phase is V 1(acetonitrile): V 2(0. 05 m o l/L K H
2P O
3): V 3(triethylamine)=8: 92: 0.18, detect wavelength 220nm, detect under the condition of flow velocity 1.0mL/min.
1.2.2 trial test
Before the trial test mice is carried out the nursing observation of 7d, in the observation period, the mice free choice feeding, drinking-water is eliminated the natural death mice.Choose 25 of healthy mices in the trial test phase, male and female half and half are divided into 5 groups at random.Every group 5, fasting is 8 hours before the test, and Herba Sophorae alopecuroidis total alkali is mixed with 5 concentration, and each organizes the isoconcentration intraperitoneal injection, determines the dosage range of formal test.
1.2.3 formal test
According to trial test, determine Dn and Dm, between Dn and Dm, establish 6 dosage groups, every group of 10 mices, male and female half and half.Fasting is 8 hours before the test, freely drinks water.Observe and record behavior state and the death condition of mice behind the intraperitoneal injection immediately, the observation period is 7 days, calculates median lethal dose(LD 50) (LD according to experimental result
50), dead mice in time to be dissected, record pathological changes situation is observed mouse peritoneal and parenchymatous organ's pathological change.
1.2.4 LD
50Computational methods
Adopt the improvement karber's method to calculate LD
50And LD
5095% fiducial limit, computing formula is as follows:
LD
50=lg
-1[Xm-?(Σp-0.5)]
Sx
50=?
LD
5095% fiducial limit=lg
-1(lgLD
50± 1.96 * Sx
50)
In the formula: be the group distance, namely the log10 dose of adjacent 2 groups of dosage is poor; Xm is maximum mortality rate group dosage logarithm; Σ p is each dosage group mortality rate sum; P is each dosage group mortality rate; Q is each dosage group survival rate; N is each treated animal number; Sx
50Be lgLD
50Standard error.
2 results and analysis
2.1 trial test result
Herba Sophorae alopecuroidis total alkali is as shown in table 2 to chmice acute toxicity trial test result.The result draws by trial test, and Dn and Dm are respectively 200mg/kg and 1000mg/kg.Determine that according to the trial test result formal test is divided into 6 dosage groups, obtain the dosage ratio by following formula:
r=(N-1)
In the formula, r is the ratio of two adjacent groups dosage; N is required group of number of formal test.Calculate the r=0.707 of formal test.
According to the trial test result, be made as 200mg/kg, 276.24mg/kg, 381.55mg/kg, 527.00mg/kg, 727.90mg/kg, 6 dosage groups of 1000mg/kg body weight, carry out acute toxicity test.The result is as shown in table 3.According to the data in the table 3, to be calculated by formula, Herba Sophorae alopecuroidis total alkali is to the median lethal dose(LD 50) LD of mice
50=559.24mg/kg, LD
50The 95% credible 471.95mg/kg～663.59mg/kg that is limited to.Therefore judge Herba Sophorae alopecuroidis total alkali to mice through lumbar injection LD
50Greater than 500mg/kg, according to China's animal toxicology acute toxicity staging, Herba Sophorae alopecuroidis total alkali belongs to low toxicity material.
2.3 Herba Sophorae alopecuroidis total alkali is to the acute toxicity test in mice clinical observation
Mice disposable celiac drug administration by injection, when dosage was 200mg/kg, mice did not have the phenomena of mortality in experimental period, and not dead mice symptom alleviated gradually in 2 hours, and basic the recovery normally can normally be taken food and drink water after 12 hours; When dosage was 276.24mg/kg, dead mouse was one in 1 hour; When dosage was 381.55mg/kg, dead mouse was 2 within 15 minutes; When dosage was 527.00mg/kg, dead mouse was 3 in 10 minutes; When dosage was 727.90mg/kg, dead mouse was 6 in 10 minutes; When dosage was 1000mg/kg, mice was all dead in 5 minutes.
The main poisoning symptom of mice behind intraperitoneal injection shows as lethargy, hogback, and whole body is twitched, and fore paw is scratched mouth, and the discontinuity excitement is arranged.On one's deathbed present jump action before, extremity are the swimming shape then more, and are dead subsequently.
2.4 the dead mice of acute toxicity test is cutd open the inspection result
Dead mice dissection is found in the abdominal cavity a small amount of hydrops is arranged, heart color and luster dimness, liver has slight enlargement, and kidney is observed no significant change, and other internal organs do not have obviously observes pathological changes.Survival mice and blank group mice are put to death also anatomic observation after 6 days, do not find seroperitoneum, other internal organs do not have obviously observes pathological changes.
3 results
Result of the test shows that Herba Sophorae alopecuroidis total alkali is to the median lethal dose(LD 50) LD of mouse peritoneal injection
50Be 559.24mg/kg, LD
5095% the credible 471.95mg/kg～663.59ma/kg that is limited to, meet the low toxicity material grade scale.
Behind the disposable mouse peritoneal drug administration by injection, survival mice is basic after 12 hours recovers normal, after 6 days survival mice is carried out Anatomical Observation, and each internal organs there is no obviously observes pathological changes.Illustrate that the survival mice recovery of test back is very fast, medicine is less to its long lasting effect.
The research of Herba Sophorae alopecuroidis total alkali on human clinical medicine is more with application, but the research aspect animal husbandry production is less with application, this test is estimated by the acute toxicity to Herba Sophorae alopecuroidis total alkali, for the further developmental research of Herba Sophorae alopecuroidis total alkali in animal husbandry later on should be used as preparation.
Endometritis, the vaginitis of embodiment 3 usefulness Herba Sophorae alopecuroidis total alkali infusion liquid treatment pig, effect is clear and definite, cures the back and is difficult for bounce-back.
From my company in 2011 to 2012 endometritis, the vaginitis of Herba Sophorae alopecuroidis total alkali infusion liquid treatment pig carried out clinical trial.Successively selected trouble endometritis, colpitic sow as subjects on pig farm, silver Jingyuan through consultation.
Wherein, suffer from 10 of chronic endometritis, colpitic sows, it is normal that clinical symptoms shows as all feelings of oestrusing, and joins infertilely repeatly, even gestation, great majority are also miscarried, and discharges the muddy mucus of floccule from vaginal orifice, particularly oestruses or discharge when keeping down more.Discharged by vaginal orifice when serious, have canescence or filemot purulent exudate or the pus of foul smell.The loss of appetite that has or useless exhausted, fervescence.
Suffer from 10 of acute endometritis, vaginitis sows, clinical symptoms shows as mucus and mucus purulence.Sick pig General Symptoms is obvious; spirit is depressed, and appetite descends even is useless exhausted, fervescence; be generally 39.5 ℃～41.5 ℃; hogback, frequent micturition is exerted duty; from vaginal orifice, constantly discharge bois de rose or yellow-white mucus frowzy or purulent secretion; it is dirty red or brown that weight person is, and discharges morely when it is droped to the ground, and often is mixed with the afterbirth fragment.
1. test and use medicine
The Herba Sophorae alopecuroidis total alkali infusion liquid: wherein the content of Herba Sophorae alopecuroidis total alkali is the 6%(mass percent).
The injection penicillin is available from local veterinary drug shop, and pharmaceutcal corporation, Ltd produces by expedition, and lot number is 20110623, and specification is that 5g/ props up.
2. test
2.1 test grouping
5 to 19 June in 2011, on this pig farm 10 ill sows that occurred clinical symptoms as first group; Have 22 ill sows that clinical symptoms occurs June 25 to July 25 in same pasture, select 10 to the similar substantially ill sow of first group of situation as second group; Every group includes 5 chronic endometritiss, colpitic sow and 5 acute endometritiss, colpitic sow, and administration Herba Sophorae alopecuroidis total alkali infusion liquid, injection ampicillin are treated respectively to two groups respectively.
2.2 test method
Test group adopts the Herba Sophorae alopecuroidis total alkali infusion liquid to treat, every sow potion connects by semen deposition bottle or enemator or syringe (100ml) and to be about the simple and easy deferent duct of 50cm or stomach catheter carries out the intrauterine perfusion, 100 milliliters of each consumptions, once a day, being used in conjunction 5d is a course for the treatment of; Matched group adopts ampicillin for inj sodium, and each 50ml is poured in the uterus, and once a day, being used in conjunction 5d is a course for the treatment of.
3 results
Cure after the medication to show as and often discharge purulent secretion from vaginal orifice, gray, yellowish-brown, canescence secretions disappear, and are adhering to purulent secretion on vaginal orifice surrounding skin and the root of the tail and are disappearing.Endometritis, colpitic cure rate with Herba Sophorae alopecuroidis total alkali infusion liquid treatment pig are 80%, and endometritis, the colpitic cure rate of ampicillin treatment pig are 70%.
10 sows of Herba Sophorae alopecuroidis total alkali infusion liquid group have endometritis, the vaginitis of 8 sows to disappear after medication 5d or obviously change, and cure rate is 80%(8/10), the not recurrence of endometritis, vaginitis.10 sows of ampicillin group have 7 endometritis, vaginitis symptom to disappear after medication or obviously change after five days, cure rate is 70%(7/10), endometritis, vaginitis rebound phenomenon are arranged in healing process, cure not thorough.The therapeutic effect of Herba Sophorae alopecuroidis total alkali infusion liquid group is higher than the ampicillin group.
4 conclusions
Herba Sophorae alopecuroidis total alkali infusion liquid, ampicillin for inj sodium treatment sow endometritis, vaginitis are respectively 87.5% and 78%, and the Herba Sophorae alopecuroidis total alkali infusion liquid is higher aspect cure rate.Aspect economic benefit, the frequent use during treating of ampicillin for inj sodium causes drug resistance easily, causes treating again more difficult; The Herba Sophorae alopecuroidis total alkali infusion liquid is as pure natural medical, and it is little to have toxic and side effects, and advantage such as be difficult for developing immunity to drugs can be saved cost significantly, and herbal treatment pays attention to conditioning, is conducive to the recovery of animal economy function and body condition.Therefore Herba Sophorae alopecuroidis total alkali infusion liquid treatment sow endometritis, vaginitis have vast potential for future development.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment the present invention is had been described in detail, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. endometritis and colpitic infusion liquid for the treatment of pig, it is characterized in that: the effective ingredient of described infusion liquid is Herba Sophorae alopecuroidis total alkali.
2. infusion liquid according to claim 1, it is characterized in that: described infusion liquid contains the Herba Sophorae alopecuroidis total alkali of mass percent 3-8%.
3. infusion liquid according to claim 1 and 2, it is characterized in that: described infusion liquid is prepared from by following steps:
1) preparation Herba Sophorae alopecuroidis total alkali: get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, the adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W filters the back and reclaims ethanol, and adding 2% hydrochloric acid accent pH is 2-5, add ether washing 2 times, obtain the filtrate precipitation, add three precipitations of chloroform extraction again, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) preparation infusion liquid: in the Herba Sophorae alopecuroidis total alkali that step 1) obtains, add the water for injection standardize solution, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, under aseptic condition, carry out fill and obtain infusion liquid.
4. treat the endometritis of pig and the preparation method of colpitic infusion liquid for one kind, step is as follows:
1) preparation Herba Sophorae alopecuroidis total alkali: get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, the adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W filters the back and reclaims ethanol, and adding 2% hydrochloric acid accent pH is 2-5, add ether washing 2 times, obtain the filtrate precipitation, add three precipitations of chloroform extraction again, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) preparation infusion liquid: in the Herba Sophorae alopecuroidis total alkali that step 1) obtains, add the water for injection standardize solution, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is (3-8): (92-97), adopt flowing steam sterilization 30-40min, under aseptic condition, carry out fill and obtain infusion liquid.
5. the preparation method of a Herba Sophorae alopecuroidis total alkali, step is as follows: get the Herba Sophorae alopecuroidis seed meal and be broken to the 50-80 order, the adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W, filter the back and reclaim ethanol, adding 2% hydrochloric acid accent pH is 2-5, adds ether washing 2 times, obtains the filtrate precipitation, add three precipitations of chloroform extraction again, the reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali.
6. Herba Sophorae alopecuroidis total alkali is in the endometritis for the treatment of pig and the application in the vaginitis.
7. the Herba Sophorae alopecuroidis total alkali infusion liquid is in the endometritis for the treatment of pig and the application in the vaginitis.
8. application according to claim 7 is characterized in that: the quality percentage composition of Herba Sophorae alopecuroidis total alkali is 3-8% in the described Herba Sophorae alopecuroidis total alkali infusion liquid.
9. application according to claim 7 is characterized in that: be the Herba Sophorae alopecuroidis total alkali infusion liquid is filled in the uterus of pig, and 100 milliliters of each consumptions, once a day.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2571236C2 (en) * | 2014-02-07 | 2015-12-20 | Общество с ограниченной ответственностью "ФОТЕК" | Method of treating infertility accompanying chronic endometritis with endometrial hypoplasia |
| CN106106363A (en) * | 2016-07-29 | 2016-11-16 | 董标 | A kind of cultural method preventing and treating broad sow parvovirus infection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998639A (en) * | 2006-01-14 | 2007-07-18 | 兰州大学第一医院 | Application sophora alopecuroide total alkali for preparing medicine to treat oral cavity herpes simplex and ulcer |
| CN102100734A (en) * | 2009-12-18 | 2011-06-22 | 武新安 | Sophora alopecuroide total alkali medicine for treating herpes zoster |
| CN103142683A (en) * | 2013-03-12 | 2013-06-12 | 广东海纳川药业股份有限公司 | Sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid for treating bovine mastitis and endometritis as well as preparation method of perfusion fluid |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998639A (en) * | 2006-01-14 | 2007-07-18 | 兰州大学第一医院 | Application sophora alopecuroide total alkali for preparing medicine to treat oral cavity herpes simplex and ulcer |
| CN102100734A (en) * | 2009-12-18 | 2011-06-22 | 武新安 | Sophora alopecuroide total alkali medicine for treating herpes zoster |
| CN103142683A (en) * | 2013-03-12 | 2013-06-12 | 广东海纳川药业股份有限公司 | Sophora alopecuroide total alkaloid-containing traditional Chinese medicine perfusion fluid for treating bovine mastitis and endometritis as well as preparation method of perfusion fluid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2571236C2 (en) * | 2014-02-07 | 2015-12-20 | Общество с ограниченной ответственностью "ФОТЕК" | Method of treating infertility accompanying chronic endometritis with endometrial hypoplasia |
| CN106106363A (en) * | 2016-07-29 | 2016-11-16 | 董标 | A kind of cultural method preventing and treating broad sow parvovirus infection |
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