CN103270893A - Method for appraising resistance of strawberries to gray mold - Google Patents
Method for appraising resistance of strawberries to gray mold Download PDFInfo
- Publication number
- CN103270893A CN103270893A CN2013102151815A CN201310215181A CN103270893A CN 103270893 A CN103270893 A CN 103270893A CN 2013102151815 A CN2013102151815 A CN 2013102151815A CN 201310215181 A CN201310215181 A CN 201310215181A CN 103270893 A CN103270893 A CN 103270893A
- Authority
- CN
- China
- Prior art keywords
- resistance
- strawberry
- gray mold
- valuables
- petal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for appraising resistance of strawberries to gray mold. The method includes the following steps: A, when flowers start to become white, selecting 20 flowers starting to become white for each variety, hanging tags on flower stalks and recording the date starting to become white, recording the number of rest petals on the flowers after becoming white, and calculating petal falling-off rate; B, after a head flower becomes white, selecting plants, inspecting all flower organs of the plants, and calculating morbidity and disease index of gray mold; C, calculating susceptibility of strawberry varieties according to a calculation formula shown as: Y=-0.248X+23.87+Z; and D, classifying resistance levels, namely, classifying the resistance levels according to susceptibility of different strawberry varieties. The method for appraising resistance of the strawberries to the gray mold is easy to implement, simple and convenient to operate, quick, accurate, capable of realizing screening and appraising of strawberry gray mold resistance in a large scale manner and suitable for resistance appraising of saprophytic or weak parasitic funguses like the gray mold, and has the advantages of stable appraisal result, high accuracy and precision and more suitability for genetic research.
Description
Technical field
The present invention relates to strawberry cultivars authenticate technology field, more specifically relate to the method that a kind of grey mould fruit rot of strawberry resistance is identified.
Background technology
Gray mold claims grape spore fruit rot again, is one of destructive disease of tool in the world wide.Cause of disease Botrytis cinerea (Botrytis cinerea) is the phorozoon of rich gram grape spore cup fungi (Botryotinia fuckeliana).Botrytis cinerea is a kind of dead volume pathogen of non-specialization, is present in hundreds of plant, mainly is the warm area of weather.Botrytis cinerea can produce scab on the organ of some plant host, and during as the growth of weak bacterial parasite or saprophytic bacteria, keeps hiding or resting state before the host is old and feeble or dead on other hosts.
Flower is the main path that Botrytis cinerea is infected strawberry fruit.Because flower is open, easily infected.Conidium is infected petal, stamen and gynoecium easily, but does not infect sepal.The floral organ that decays is the main source that mycelium infects fruit in the growth (holder).Fruit also can be infected by the mycelium in the petal, and during fruit maturation, pathogen grows, occurs symptom rapidly surely and produces spore on fruit.But it is generally acknowledged that conidium is to the not too important (Braui of directly infecting of healthy fruit, P.G.and Sutton, J.C.1987.Inoculum sources of Botrytis cinerea in fruit rot of strawberry in Ontario.Can.J.Plant pathol.9:1-5).
Because the saprophytic characteristic of Botrytis cinerea adopts conventional resistance authentication method to be difficult to the gray mold resistance level of strawberry cultivars is accurately identified.For this reason, a kind of resistance of gray mold accurately and effectively authentication method of invention is extremely important.
Summary of the invention
Because the saprophytic and weak parasitic character of botrytis cinerea, present still do not have the method that can effectively identify the grey mould fruit rot of strawberry resistance.The objective of the invention is to be to provide a kind of method of grey mould fruit rot of strawberry resistance evaluation, easy to implement the method, easy and simple to handle, the extensive Screening and Identification grey mould fruit rot of strawberry resistance of accurate and energy fast,, further crossbreeding or molecular mark provide technical support, are applicable to that more disease resistance saprophytic or weak parasitical fungi such as grey mold identifies, it is stable to have a qualification result, accuracy and accuracy height are more suitable for the characteristics of genetics research.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions:
The method that a kind of grey mould fruit rot of strawberry resistance is identified (identifying that strawberry is to the method for gray mold resistance) may further comprise the steps:
A, when flower has just begun to show money or valuables one carries unintentionally, each kind is selected 20 flowers that just begun to show money or valuables one carries unintentionally, and hangs the date that drop and record show money or valuables one carries unintentionally anthocaulus.The residue petal number that shows money or valuables one carries unintentionally and recorded on the flower in back 7 days calculates the petal expulsion rate.Petal expulsion rate computing formula is:
Wherein 20 is the flower quantity of investigating; A is the average petal number of strawberry floral organ.
B, showed money or valuables one carries unintentionally back 14 days in headdress flower, select the representational plant of 3 strains, the floral organs all to three strain plant carry out
Investigation, the statistics gray mold incidence of disease and disease index;
The susceptibility of C, calculating strawberry cultivars;
Susceptibility computing formula: Y=-0.248X+23.87+Z
Y: susceptibility;
X: 7 days expulsion rates of petal;
Z: showed money or valuables one carries unintentionally 14 days, disease index is in the floral organ ratio more than 5 grades.
D, resistance level classification: the susceptibility according to different strawberry cultivars is classified criteria for classification to its resistance level
As follows:
Described floral organ gray mold disease index statistical method is as follows:
0 grade: the healthy no obvious variable color of floral organ;
1 grade: sepal reddens the look area between 0-10%;
3 grades: sepal reddens the look area between 10-50%;
5 grades: sepal reddens the look area between 50-100% or sepal becomes the withered area of sepia and is 0-10%;
7 grades: it is 10-100% that sepal becomes brown withered area.
Compared with prior art, the invention has the beneficial effects as follows:
1. the gray mold resistance of each kind is identified that the incidence of plant is subject to the influence of environmental condition in the qualification process of field, resistance qualification result instability by the gray mold incidence of observing fruit and floral organ in the existing strawberry breeding for disease resistance process.The expulsion rate of petal was identified the gray mold resistance of strawberry cultivars after the present invention showed money or valuables one carries unintentionally 7 days according to the strawberry floral organ.The expulsion rate of petal was relevant with the floral organ structure of plant after the strawberry floral organ showed money or valuables one carries unintentionally 7 days, controlled by the plant corresponding gene, had the influence that genetic stability is not subject to environmental condition.
2. need the gray mold disease index of strawberry fruit is investigated in the existing strawberry breeding for disease resistance process, just can carry out corresponding investigation after need waiting until fruit maturation, length expends time in.Because botrytis cinerea infects the characteristic of fruit by the strawberry floral organ, and the remarkable negative correlation of the incidence of disease of petal falling speed and fruit.The expulsion rate of petal reflected the gray mold resistance of strawberry cultivars after the present invention showed money or valuables one carries unintentionally 7 days by the statistics floral organ, saved time.
3. need floral organ the inoculation of exsomatizing to identify in the existing strawberry breeding for disease resistance process, expend time in and manpower.The present invention can carry out in existing breeding nursery, need not carry out artificial infection etc.The expulsion rate of petal and the gray mold resistance that these two indexs of floral organ susceptibility just can be determined kind had the advantage that uses manpower and material resources sparingly after only the floral organ of need description plant showed money or valuables one carries unintentionally 7 days.
Description of drawings
Fig. 1 for the strawberry floral organ showed money or valuables one carries unintentionally 7 days after petal expulsion rate and fruit gray mold incidence of disease correlation analysis.
The result of Fig. 1 show the strawberry floral organ showed money or valuables one carries unintentionally 7 days after petal expulsion rate and the remarkable positive correlation of the fruit gray mold incidence of disease, this reflects, and to have demonstrate,proved botrytis cinerea mainly be to infect the infection fruit from strawberry petal.Illustrated also simultaneously that the petal expulsion rate reflects that the method for the gray mold resistance of strawberry cultivars is reliable after showing money or valuables one carries unintentionally 7 days by mensuration strawberry floral organ.
Embodiment
Embodiment 1:
The method that a kind of grey mould fruit rot of strawberry resistance is identified the steps include:
1, vegetable material: the cultivated strawberry kind for the different resistances of the botrytis cinerea that tries is: sweet profit, brilliant jade, brilliant precious jade, Feng Xiang, red cheek totally 5 kinds of looking into;
2, plant trait investigation: each kind is selected 20 flowers that just showed money or valuables one carries unintentionally, and hangs on the anthocaulus with white drop, on the date that the drop record shows money or valuables one carries unintentionally.Showed money or valuables one carries unintentionally back 7 days, 14 days, 21 days, 28 days, and recorded petal quantity residual on the floral organ respectively, calculate the petal expulsion rate;
3, floral organ showed money or valuables one carries unintentionally back 14 days, adopted 5 point samplings that the incidence of flower is investigated.3 strawberry plant of each point selection carry out investigation statistics to all the flower incidences on it;
4, after fruit is finished annesl, adopt the 5 point samplings investigation fruit incidence of disease.Each kind is selected 5 points, every point selection
50 fruits of annesl, investigation fruit incidence;
5, showed money or valuables one carries unintentionally 7 days by floral organ after petal expulsion rate and the floral organ incidence of disease calculate the susceptibility of different strawberry cultivars respectively; According to the susceptibility of each kind, with the gray mold resistance level of each kind be divided into immunity, high anti-, in anti-, middle sense and five kinds of levels of high sense.
Strawberry floral organ gray mold disease index grade scale:
0 grade: the healthy no obvious variable color of floral organ;
1 grade: sepal reddens the look area between 0-10%;
3 grades: sepal reddens the look area between 10-50%;
5 grades: sepal reddens the look area between 50-100% or sepal becomes the withered area of sepia and is 0-10%;
7 grades: it is 10-100% that sepal becomes brown withered area.
Strawberry fruit gray mold disease index grade scale:
0 grade: the healthy gray mold of fruit infects;
1 grade: the area that fruit is infected by gray mold accounts for the ratio of whole fruit area less than 25%;
2 grades: the ratio that the area that fruit is infected by gray mold accounts for whole fruit area greater than 25% less than 50%;
3 grades: the ratio that the area that fruit is infected by gray mold accounts for whole fruit area greater than 50% less than 75%;
4 grades: the ratio that the area that fruit is infected by gray mold accounts for whole fruit area greater than 75% less than 100%;
Test results and analysis
1, petal dropping situations
The petal expulsion rate of different time after table 1, each strawberry cultivars floral organ show money or valuables one carries unintentionally
As can be seen from Table 1, the petal expulsion rate of the brilliant precious jade of strawberry cultivars just reaches 99% after floral organ showed money or valuables one carries unintentionally 7 days, and most petals of floral organ all come off.The petal expulsion rate of brilliant jade, Feng Xiang, red cheek is between brilliant precious jade and sweet looking between the profit, and the petal expulsion rate of all investigation kinds all reaches more than 90% in the time of the 28th day.
Table 2, different strawberry cultivars floral organ incidence
Can influence fruit-setting rate and influence ultimate output thereby the floral organ of strawberry is subjected to botrytis cinerea to infect morbidity.As can be seen from Table 2, the floral organ of the brilliant precious jade of strawberry cultivars, Feng Xiang, red cheek is easily susceptible, and botrytis cinerea easily infects the floral organ of brilliant precious jade and causes the floral organ morbidity, and the brilliant beautiful and sweet floral organ of looking into profit of strawberry cultivars then infects immunity to botrytis cinerea.
Table 3, different strawberry cultivars fruit incidence
As can be seen from Table 3, the ratio of the brilliant precious jade of strawberry cultivars, brilliant beautiful, Feng Xiang and red cheek disease plant all is lower than 9%, and the ratio of morbidity fruit all to be lower than be 4%; Disease index is lower than 0.21; The sweet fruit of looking into profit is fallen ill comparatively serious, and the fruit morbidity is arranged on 56% the plant, and morbidity fruit ratio is 19%, and disease index is 11%.This shows that the sweet fruit of looking into profit is fallen ill the most serious, brilliant precious jade, brilliant jade, Feng Xiang and the morbidity of red cheek are gently.
According to Fig. 1 as can be known, different strawberry cultivars floral organs were showed money or valuables one carries unintentionally 7 days after the correlation analysis of petal expulsion rate and the fruit incidence of disease show: the remarkable negative correlation of petal expulsion rate (Fig. 1) after the fruit incidence of disease of strawberry cultivars and floral organ showed money or valuables one carries unintentionally 7 days.Botrytis cinerea mainly infects the strawberry floral organ by stamen, gynoecium and petal, hides in floral organ, treats the ripe postoperative infection fruit of fruit sense.After blooming, developed area ratio stamen and the gynoecium of petal are bigger, are easily infected surely by botrytis cinerea and grow, and coming off fast of petal helps to reduce gray mold infects fruit by floral organ probability on the floral organ; Simultaneously, floral organ infects gray mold can bear fruit in direct influence, and floral organ gray mold disease index can not normally be beared fruit in time more than 5 grades.Therefore in grey mould fruit rot of strawberry resistance qualification process, need take all factors into consideration the show money or valuables one carries unintentionally gray mold resistance level of back 7 days petal expulsion rates and floral organ of strawberry floral organ.For this reason, the susceptibility of the disease resistance COMPREHENSIVE CALCULATING plant by petal expulsion rate and floral organ reflects that with this method of plant resistance to environment stress level is rational.
Susceptibility (Y) computing formula: Y=-0.247X+23.87+Z
Y: susceptibility;
X: petal expulsion rate after floral organ showed money or valuables one carries unintentionally 7 days;
Z: after floral organ showed money or valuables one carries unintentionally back 14 days, disease index was in the floral organ ratio more than 5 grades.
Table 4,5 strawberry cultivars disease resistances are estimated
Table 5, the classification of grey mould fruit rot of strawberry resistance
Claims (1)
1. the method that the grey mould fruit rot of strawberry resistance is identified the steps include:
1) when flower begins to show money or valuables one carries unintentionally, each kind is selected 20 flowers that begin to show money or valuables one carries unintentionally, and hangs drop anthocaulus and also records the date that shows money or valuables one carries unintentionally, and the residue petal number that shows money or valuables one carries unintentionally and recorded on the flower in back 7 days calculates the petal expulsion rate, and petal expulsion rate computing formula is:
Wherein 20 is the flower quantity of investigating; A is the average petal number of strawberry floral organ;
2) showed money or valuables one carries unintentionally back 14 days in headdress flower, select the plant of 3 strains, all floral organs of three strain plant are investigated, the statistics gray mold incidence of disease and disease index;
3) susceptibility of calculating strawberry cultivars;
Susceptibility computing formula: Y=-0.248X+23.87+Z
Y: susceptibility;
X: 7 days expulsion rates of petal;
Z: showed money or valuables one carries unintentionally 14 days, disease index is in the floral organ ratio more than 5 grades;
4) resistance level classification: the susceptibility according to different strawberry cultivars is classified to its resistance level, and criteria for classification is as follows:
Described floral organ gray mold disease index statistical method is as follows:
0 grade: the healthy no obvious variable color of floral organ;
1 grade: sepal reddens the look area less than 10%;
3 grades: sepal reddens the look area between 10-50%;
5 grades: sepal reddens the look area between 50-100% or sepal becomes the withered area of sepia less than 10%;
7 grades: sepal becomes the withered area of sepia greater than 10-100%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310215181.5A CN103270893B (en) | 2013-05-31 | 2013-05-31 | Method for appraising resistance of strawberries to gray mold |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310215181.5A CN103270893B (en) | 2013-05-31 | 2013-05-31 | Method for appraising resistance of strawberries to gray mold |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103270893A true CN103270893A (en) | 2013-09-04 |
| CN103270893B CN103270893B (en) | 2014-06-25 |
Family
ID=49053141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310215181.5A Active CN103270893B (en) | 2013-05-31 | 2013-05-31 | Method for appraising resistance of strawberries to gray mold |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103270893B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191682A (en) * | 2015-10-27 | 2015-12-30 | 河南省农业科学院植物保护研究所 | Disease resistance identification method for phoma arachidicola |
| CN105557347A (en) * | 2014-10-13 | 2016-05-11 | 江西省农业科学院植物保护研究所 | Gray mold resistance identified seedling stage inoculation method for capsicum |
| CN108693308A (en) * | 2018-04-12 | 2018-10-23 | 湖北省农业科学院经济作物研究所 | A kind of method of Fast Evaluation strawberry cultivars petal falling speed |
| CN108913751A (en) * | 2018-06-22 | 2018-11-30 | 湖北省农业科学院经济作物研究所 | A method of evaluation strawberry stamen gray mold resistance |
| CN110904186A (en) * | 2019-12-30 | 2020-03-24 | 云南农业大学 | Identification method for disease resistance of Chinese rose gray mold |
| CN114830974A (en) * | 2022-06-02 | 2022-08-02 | 遵义市农业科学研究院 | Strawberry powdery mildew green prevention and control method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1623371A (en) * | 2003-12-05 | 2005-06-08 | 东北农业大学 | Method for selecting tomato seed for resisting multi-disease and resurvey |
-
2013
- 2013-05-31 CN CN201310215181.5A patent/CN103270893B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1623371A (en) * | 2003-12-05 | 2005-06-08 | 东北农业大学 | Method for selecting tomato seed for resisting multi-disease and resurvey |
Non-Patent Citations (1)
| Title |
|---|
| 施守华; 胡长安; 韦永保; 刘明熙; 杜桂丽: "保护地草莓灰霉病发生规律及防治技术", 《安徽农学通报》, 30 June 2006 (2006-06-30), pages 181 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557347A (en) * | 2014-10-13 | 2016-05-11 | 江西省农业科学院植物保护研究所 | Gray mold resistance identified seedling stage inoculation method for capsicum |
| CN105557347B (en) * | 2014-10-13 | 2018-11-20 | 江西省农业科学院植物保护研究所 | The Seedling Inoculation method of Botrytis cinerea Disease Resistance Identification |
| CN105191682A (en) * | 2015-10-27 | 2015-12-30 | 河南省农业科学院植物保护研究所 | Disease resistance identification method for phoma arachidicola |
| CN108693308A (en) * | 2018-04-12 | 2018-10-23 | 湖北省农业科学院经济作物研究所 | A kind of method of Fast Evaluation strawberry cultivars petal falling speed |
| CN108913751A (en) * | 2018-06-22 | 2018-11-30 | 湖北省农业科学院经济作物研究所 | A method of evaluation strawberry stamen gray mold resistance |
| CN108913751B (en) * | 2018-06-22 | 2021-04-27 | 湖北省农业科学院经济作物研究所 | Method for evaluating gray mold resistance of strawberry stamen |
| CN110904186A (en) * | 2019-12-30 | 2020-03-24 | 云南农业大学 | Identification method for disease resistance of Chinese rose gray mold |
| CN114830974A (en) * | 2022-06-02 | 2022-08-02 | 遵义市农业科学研究院 | Strawberry powdery mildew green prevention and control method |
| CN114830974B (en) * | 2022-06-02 | 2024-02-02 | 遵义市农业科学研究院 | Green prevention and control method for powdery mildew of strawberries |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103270893B (en) | 2014-06-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103270893B (en) | Method for appraising resistance of strawberries to gray mold | |
| James | Sugarcane | |
| Lofgren et al. | Ectomycorrhizal host specificity in a changing world: can legacy effects explain anomalous current associations? | |
| Baloch et al. | Yield related morphological measures of short duration cotton genotypes. | |
| CN102220407B (en) | Method for identifying gray mold resistance of gerbera | |
| Cadle-Davidson et al. | Natural infection of Run1-positive vines by naïve genotypes of Erysiphe necator | |
| Silva et al. | Heterosis and combining ability for ornamental chili pepper | |
| Miles et al. | Yield, labor, and fruit and juice quality characteristics of machine and hand-harvested ‘Brown Snout’specialty cider apple | |
| Ekanayake et al. | Varietal classification of new coconut (Cocos nucifera L.) forms identified from Southern Sri Lanka | |
| CN110149947B (en) | Screening research method for tobacco field thrips resistance characters | |
| Almeida et al. | Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.) | |
| CN105142395B (en) | The cucumber plant of dark stem | |
| MIKALIŪNIENĖ et al. | Evaluation of red clover (Trifolium pratense L.) resistance to Sclerotinia crown and root rot (Sclerotinia trifoliorum) in the laboratory and field conditions. | |
| CN109329046B (en) | Breeding method of Chancalyx and TYLCV-resistant cherry tomato inbred line | |
| CN109628549A (en) | Using excised leaf Rapid identification capsicum single plant to the method for glue spore resistance toanthracnose | |
| Buchholz | Studies concerning the evolutionary status of polycotyledony | |
| Michailides et al. | Conventional and molecular assays aid diagnosis of crop diseases and fungicide resistance | |
| Sima et al. | In vitro evaluation of resistant of potato cultivars against black leg disease (Pectobacterium atrosepticum) | |
| CN108913751B (en) | Method for evaluating gray mold resistance of strawberry stamen | |
| Anh et al. | Evaluating agronomic characteristics of twelve local papaya (Carica papaya L.) varieties in Viet Nam | |
| Alima et al. | Influence of Fusarium oxysporum-resistant pumpkin rootstock on the productivity of the F1 Asylum cucumber hybrid | |
| Abdullah | Diversity and roles of mycorrhizal fungi in the bee orchid Ophrys apifera | |
| CN109601172A (en) | A kind of fruit thinning method of Early maturity satsuma orange | |
| Solberg et al. | Morphological and phenological diversity in Scandinavian wild carrot. | |
| Khalil et al. | Selection of some promising guava trees grown in Kafr El-Sheikh governorate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |