CN103251890B - Plant essential oil composition and anxiolytic effect thereof - Google Patents
Plant essential oil composition and anxiolytic effect thereof Download PDFInfo
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Abstract
The invention discloses a plant essential oil composition and an anxiolytic effect thereof. The plant essential oil composition consists of the following components in parts by volume: 20-80 parts of cassia twig oil, 20-80 parts of cupressin, 20-80 parts of juniper wood oil and 5-20 parts of a component B essential oil; the component B essential oil consists of the following supercritical extracts in parts by weight: 10 parts of fennel seed, 10 parts of schisandra chinensis, 10 parts of costus root, 5 parts of rhizoma atractylodis, 10 parts of curcuma aromatica, 10 parts of wild chrysanthemum flower, 20 parts of pogostemon cablin, 5 parts of sandalwood, 10 parts of rhizoma ligustici wallichii, 10 parts of red sage root, 20 parts of cassia seed, 5 parts of frankincense, 5 parts of myrrh, 5 parts of agilawood and 20 parts of rhizoma cyperi. According to the plant essential oil composition, the cassia twig oil, the cupressin, the uniper wood oil and the component B essential oil are screened out from a plurality of plant essential oils and are mixed together in proportion to prepare the plant essential oil composition which can be used as an external medicament for preventing and treating anxiety neurosis. The plant essential oil composition is derived from pure natural plants, is nontoxic and harmless, and is simple in preparation process.
Description
Technical field
The present invention relates to a kind of plant essential oil composition and angst resistance effect thereof.
Background technology
Anxiety is a kind ofly to take anxiety and be main neurosis, and main manifestations is the anxiety, anxiety of ictal or persistence, the terrified anxiety such as uneasy, and with symptoms such as autonomic nervous dysfunction, muscular tone and motion uneasinesses.Anxiety or a kind of disease long-term, chronic, that repeatedly show effect, exist the abnormal of psychosoma two aspects.The anxiety symptom of anxiety neurosis is former, and other is caused by emotional factor, take somatization as main manifestations, with the disease of anxiety symptom, should belong to the content of psychosomatic disease, is called anxious syndrome.Anxiety neurosis forms anxiety neurosis (anxiety disorder) by generalized anxiety disorder (GAD) and acute anxiety attack (PD).Generalized anxiety disorder claims again chronic anxiety, be a kind of lack clear and definite object and particular content continue on tenterhooks and nervous be main anxiety neurosis; And there are significant autonomic nerve symptom, muscular tone and mobility uneasy.Patient cannot free and agonize again because of endurable.
Clinically generally show as patient mentally and often feel without obvious cause, the anxiety without clear and definite object and immobilized substance, agitation and nervous.Arousal level is high, is often great vigilance state, on tenterhooks, as excessive worry and care surroundings, easily enrages, with sleep disorder.There is perspiration, xerostomia, flushed face or pale, dizzy, uncomfortable in chest, cardiopalmus, dyspnea, frequent micturition, urgent micturition etc. in vegetative nerve aspect.External body occurs that motor type is uneasy: muscular tone, tremble, be on tenterhooks, often accompany muscular soreness and the myasthenia of limbs sense at head and neck, position, back.
The epidemiologic data of anxiety neurosis is due to the continuous variation of social concerns degree and diagnostic criteria, and the survey data that country variant and time carry out changes greatly.The epidemiological study discovery of the U.S., in crowd, lifetime prevalence is 4%～6%, and women is male's twice.According to U.S.'s epidemic diseases zone of responsibility research, find, anxiety neurosis produces harmful effect to 5.1% people in 15～54 years old crowd of the U.S..Anxiety patient mostly is unmarried or divorcee, and most anxiety neurosis at least exist a kind of obstacle of other physically and mentally healthy aspects.
According to the prediction of World Health Organization (WHO), psychiatry and psychohygiene will more and more be subject to people's attention, and at ten kinds, cause in the heaviest disease of burden on society, and spiritual aspect disease has just accounted for four kinds.Total burden of mental sickness accounts for 1/4th of whole disease burdens.Anxiety is the common sympton of one of mental disorder five large syndromes, is a neurosal disease independently.Along with Chinese social development, and the raising of people to self-mental health degree of concern, more and more because suffering from the number that anxiety neurosis initiatively seeks medical advice, anxiety neurosis has become one of common mental disorder of general hospital outpatient service, psychological counselling and treatment mechanism and mental hospital.Nineteen eighty-two China sick learning of 12 Local spirit disease popularities investigated demonstration, and in 1 5～59 years old population, the prevalence of anxiety neurosis is 1.48%, and Tianjin City investigation is 1.52%, and women's prevalence is apparently higher than male.China recently studies and shows, in general population, the sickness rate of anxiety neurosis is 2.0%～4.7%, and some specific crowd (as patients with coronary heart disease and student etc. before the examination) sickness rate can be up to more than 1 0%.At present, modern medicine is mainly pharmaceutical intervention and mental regulation two aspects to the treatment of anxiety neurosis.Though Drug therapy can obtain certain curative effect, there is addiction, drug resistance is stronger, the shortcoming that side effect is more.
Although Chinese Traditional Medicine does not also form independently psychosis subject, the treatment of mental sickness has been had a long history and abundant document description.The advantage of Chinese medicine anxiety neurosis: have a long history, accumulated a large amount of invaluable experiences.By integrally-regulated and because of the theory that treats of card, in treatment anxiety neurosis primary disease, to many psychosomatic diseasies with anxiety symptom, as diseases such as hypertension, gastric ulcer, functional dyspepsias, all tool has significant therapeutic effect.
Chinese medicine also has many-sided feature in the dosage form of medicine in addition, and for example inner disease outer treat is just often obtained beyond thought good effect.Aromatotherapy can be summarized as three kinds to the using method with quintessence oil and sucks, massages and absorb.Suction is to make quintessence oil enter the fastest method of human body, by nasal cavity, aromatic essential oil molecule is sucked, make quintessence oil molecule by nasal meatus, from throat rear portion, pass to the nasal epithelium being formed by olfactory receptor cell on nasal cavity top, remake the olfactory region of using brain, inspire the release of neurochemical, then via brain nervus centralis, send instruction, remove regulation and control and balance autonomic nervous system, thereby generation is calmed, loosened, joyful or excited effect.Massage is a kind of therapy of the most comfortable in aromatotherapy.Because the structure of quintessence oil molecule is less, skin permeation very easily, massage can help quintessence oil molecule to penetrate in body, accelerates to absorb, and makes body and mind loose, maintains skin simultaneously, and can active blood, promotes lymph circulation, enhancing immunity.Picked-up refers to quintessence oil is directly used in to affected part, such as clean, the antiinflammatory of wound, and the reparation of cicatrix etc.In use suck and massage more common.
External utilization aromatotherapy has had history for a long time, the most remarkable for neural effect, quintessence oil can make people mind at rest, alleviate the emotion of melancholy, uneasiness and anxiety, for allaying tiredness and relieving insomnia, also there is certain effects, simultaneously can also suppress spirit and be overexcited, contribute to focus one's attention on, the effect such as hypermnesis, contribute to alleviate anxiety neurosis.
We take traditional Chinese medicine theory as guidance, analyze the document of the Medicine and Surgery disease relevant to anxiety neurosis, use for reference doctor's clinical practice experience at all times, and domestic and international up-to-date bio-pharmaceuticals engineering, design the scientific research prescription for the treatment of anxiety neurosis, and be prepared into a kind of essential oil composition that can external.Can absorb and hear perfume (or spice) by percutaneous reaches the good prevention effect of anxiety neurosis.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of plant essential oil composition and angst resistance effect thereof are provided.
To achieve these goals, the present invention adopts following technical scheme:
A plant essential oil composition, is comprised of following component and percent by volume:
Cassia twig oil 20-40%,
Resina Cupressi 20-40%,
Chinese juniper oil 20-40%,
B component quintessence oil 5-20%;
Described B component quintessence oil is the supercritical extract of following weight parts component:
20 parts of 10 parts of Fructus Foeniculi, 10 parts of Fructus Schisandrae Chinensis, 10 parts of Radix Aucklandiae (Radix Vladimiriae), 5 parts of Rhizoma Atractylodis, 10 parts of Radix Curcumaes, 10 parts of Flos Chrysanthemi Indicis, 20 parts of Herba Pogostemonis, 5 parts, Lignum Santali Albi, 10 parts of Rhizoma Chuanxiongs, 10 parts of Radix Salviae Miltiorrhizaes, 20 parts of Semen Cassiaes, 5 parts of Olibanums, 5 parts of Myrrhas, 5 parts of Lignum Aquilariae Resinatum and Rhizoma Cyperis.
In above-mentioned plant essential oil composition, the supercritical extraction method of described B component quintessence oil is: raw material is pulverized, and crosses 40-60 mesh sieve, coordinates by weight and mixes; Pack extraction kettle into, sealing, the parameters of debugging extraction kettle, separating still, heating apparatus and refrigerating plant are normally worked, and open compressor pump and are pressurised into 21-22Mpa, pumping liquid CO
2extract plants essential oil, from separating still, resolve extract, obtain B component quintessence oil.
The using method of above-mentioned plant essential oil composition, comprises the steps: by plant essential oil composition Shi Liang Tu more than ， And massaged for 15 seconds gently around wind pond, temple, Silk bamboo cave, Baihui, windy mansion Huo Shenque acupuncture point every day 1-6 time.
Compared with prior art, the present invention has following beneficial effect:
The present invention is through great many of experiments, from numerous plants essential oils, filter out cassia twig oil, Resina Cupressi, Chinese juniper oil and
B component quintessence oil; B component quintessence oil is the supercritical extract being mixed in proportion by plurality of Chinese.Cassia twig oil, Resina Cupressi, Chinese juniper oil and B component quintessence oil are mixed the plant essential oil composition forming in proportion can be as preventing and treating the medicine for external use of anxiety neurosis.Plant essential oil composition of the present invention derives from pure natural plant, nontoxic, and preparation technology is simple.
The specific embodiment
Embodiment 1
The preparation of B component quintessence oil: various material of vegetable origins are pulverized, crosses 60 mesh sieves.Ratio is joined even by weight: 10 parts of Fructus Foeniculi, 10 parts of Fructus Schisandrae Chinensis, 10 parts of Radix Aucklandiae (Radix Vladimiriae), 5 parts of Rhizoma Atractylodis, 10 parts of Radix Curcumaes, 10 parts of Flos Chrysanthemi Indicis, 20 parts of Herba Pogostemonis, 5 parts, Lignum Santali Albi, 10 parts of Rhizoma Chuanxiongs, 10 parts of Radix Salviae Miltiorrhizaes, 20 parts of Semen Cassiaes, 5 parts of Olibanums, 5 parts of Myrrhas, 5 parts of Lignum Aquilariae Resinatum, 20 parts of Rhizoma Cyperis.Take 2 kilograms, the raw material powder that mixes, pack extraction kettle into, sealing, is adjusted to 45 ℃ by extractions temperature, and knockout tower temperature is adjusted to 55 ℃, extraction pressure 30Mpa, and parsing pressure is 5Mpa, CO
2send into depurator, by preheater, arrive assigned temperature and pressure, flow velocity 30kg/h, opens pressure regulator valve, by pressure regulator valve, controls above-mentioned parameter, dynamic extraction 3 hours.After shutdown, emit extract, be weighed as 73 grams.
Embodiment 2
The preparation of a plant essential oil composition: cassia twig oil 40%, Resina Cupressi 30%, Chinese juniper oil 20%, and the resulting B component of embodiment 1 quintessence oil 10%, by volume percentage is added and mixed and get final product.
Embodiment 3
The preparation of No. two plant essential oil compositions: Chinese juniper oil 38%, cassia twig oil 30%, Resina Cupressi 20% and the resulting B component of embodiment 1 quintessence oil 12%, by volume percentage is added and is mixed and get final product.
Embodiment 4
No. one plant essential oil composition is investigated the safety of skin: comprise animal skin acute toxicity test, irritation test and skin anaphylactic test.
Experiment material
A plant essential oil composition is by oneself preparation.Laboratory animal new zealand rabbit, regular grade, body weight (2.O ± 0.2) kg, male and female dual-purpose.Cavia porcellus, body weight 250～300g male and female dual-purpose, is provided by Guangdong Province's Experimental Animal Center.
Experimental technique and result
20 of the new zealand rabbits of body weight (2.O ± 0.2) kg are got in skin acute toxicity test, male and female half and half, and 24h before administration, by spinal column both sides, back unhairing, every about 150cm of unhairing area
2(be about body surface area 10%).Be divided into 5 groups, 4 every group, every of matched group is coated with normal saline lml; Each two groups of high low dose group (being respectively intact skin group and damaged skin group).Wherein damaged skin is sterilized skin of unhairing scratch with pin.Take oozing of blood as degree.On experimental group skin of unhairing, be coated with combined plant quintessence oil No. one, every of low dose group is coated with a combined plant quintessence oil 2.0ml, every of high dose group is coated with a plant essential oil composition 4.0ml, evenly be applied to depilation district, and with nonirritant gauze, immobilization with adhesive tape, sub-cage rearing, after administration 24h, with warm water by left drug eccysis, remove after medicine 1,24,48,72h to the 7 days, observe the situations such as rabbit body weight, skin, hair, eye, mucosa and breathing, activity, be showed no extremely, without dead, there is not notable difference with matched group.A plant essential oil composition is very little to new zealand rabbit skin acute toxicity, the actual safety of using of prompting.
Irritation test is got 20 of body weight (2.0 ± 0.2) kg new zealand rabbits, male and female half and half, and 24h before administration, by spinal column both sides, back unhairing, every about 3cm * 3cm of unhairing area.Adopt consubstantiality left and right sides Self-control method, be divided into two groups, 4 every group, skin complete group and skin injury group, rabbit right side is coated with normal saline 1ml, and left side is coated with a plant essential oil composition 2.0ml.Skin injury group is sterilized skin of unhairing scratch with pin, take oozing of blood as degree.After coating, with two-layer gauze 2.5cm * 2.5cm and one deck cellophane, cover, then with nonirritant adhesive plaster and binder, fixed sub-cage rearing.After administration 24h, with warm water by left drug eccysis, remove after medicine 0.5,1,24,48,72h observes and record and smear position and have or not erythema and edema, by skin irritation reaction, generally commonly uses standards of grading judge, records each group at score value and the recovery situation of different time.A plant essential oil composition is showed no other irritative response to skin complete group and skin injury group as a result.
Skin anaphylactic test is got 30 of healthy albino guinea-pigs, male and female half and half, and body weight 250 1 300g, before administration, 24h is by every guinea pig back both sides unhairing (3cm * 3cm).Laboratory is divided into three groups by plucked Cavia porcellus, tested medicine group, blank group (giving normal saline) and positive controls (1%2,4 one nitrochlorobenzol), 10 every group (each 5 of male and female).Sensitization contact: No. one plant essential oil composition 1.0ml is applied to animal unhairing district, left side and covers with one deck cellophane and two-layer gauze, then uses nonirritant immobilization with adhesive tape, continues 6h.The 7th day and the 14th day in kind respectively repeats sensitization once.Blank group and positive controls give respectively relative medicine with method and carry out sensitization contact.Excite contact: 14d is applied to unhairing district, guinea pig back right side by relative medicine 1.0ml after last administration, after 6h, removing medicine is observable, then in 24,48,72h observes skin allergy situation again.All there is not the serious systemic anaphylaxises such as erythema, edema, asthma, astasia or shock in the tested medicine group of result and blank group, without sensitization.All there is obvious poisoning erythema edema in positive controls, reflection meansigma methods is 4, and sensitization rate 100% is extremely sensitivity.Experimental result show plant essential oil composition to Cavia porcellus without skin allergy, very little on body immune system impact.
Through skin acute toxicity, irritation test and skin anaphylactic test, result show plant essential oil composition to rabbit without obvious acute toxic reaction, to rabbit intact skin group and the equal nonirritant of damaged skin group, to Cavia porcellus without skin allergy.Illustrating that this preparation toxicity is very little, is the good external essence oil of a kind of safety.
Embodiment 5
No. two plant essential oil composition is investigated the safety of skin: comprise animal skin acute toxicity test, irritation test and skin anaphylactic test.The experiment of embodiment 5 is identical with the process of the test of embodiment 4, and No. two plant essential oil composition component ratios change a little but quintessence oil consumption is constant.
Experiment material
No. two plant essential oil compositions are by oneself preparation.Laboratory animal new zealand rabbit, regular grade, body weight (2.O ± 0.2) kg, male and female dual-purpose.Cavia porcellus, body weight 250～300g male and female dual-purpose, is provided by Guangdong Province's Experimental Animal Center.
Experimental technique and result
20 of the new zealand rabbits of body weight (2.O ± 0.2) kg are got in skin acute toxicity test, male and female half and half, and 24h before administration, by spinal column both sides, back unhairing, every about 150cm of unhairing area
2(be about body surface area 10%).Be divided into 5 groups, 4 every group, every of matched group is coated with normal saline lml; Each two groups of high low dose group (being respectively intact skin group and damaged skin group).Wherein damaged skin is sterilized skin of unhairing scratch with pin.Take oozing of blood as degree.On experimental group skin of unhairing, be coated with combined plant quintessence oil No. one, every of low dose group is coated with a combined plant quintessence oil 2.0ml, every of high dose group is coated with a plant essential oil composition 4.0ml, evenly be applied to depilation district, and with nonirritant gauze, immobilization with adhesive tape, sub-cage rearing, after administration 24h, with warm water by left drug eccysis, remove after medicine 1,24,48,72h to the 7 days, observe the situations such as rabbit body weight, skin, hair, eye, mucosa and breathing, activity, be showed no extremely, without dead, there is not notable difference with matched group.A plant essential oil composition is very little to new zealand rabbit skin acute toxicity, the actual safety of using of prompting.
Irritation test is got 20 of body weight (2.0 ± 0.2) kg new zealand rabbits, male and female half and half, and 24h before administration, by spinal column both sides, back unhairing, every about 3cm * 3cm of unhairing area.Adopt consubstantiality left and right sides Self-control method, be divided into two groups, 4 every group, skin complete group and skin injury group, rabbit right side is coated with normal saline 1ml, and left side is coated with a plant essential oil composition 2.0ml.Skin injury group is sterilized skin of unhairing scratch with pin, take oozing of blood as degree.After coating, with two-layer gauze 2.5cm * 2.5cm and one deck cellophane, cover, then with nonirritant adhesive plaster and binder, fixed sub-cage rearing.After administration 24h, with warm water by left drug eccysis, remove after medicine 0.5,1,24,48,72h observes and record and smear position and have or not erythema and edema, by skin irritation reaction, generally commonly uses standards of grading judge, records each group at score value and the recovery situation of different time.A plant essential oil composition is showed no other irritative response to skin complete group and skin injury group as a result.
Skin anaphylactic test is got 30 of healthy albino guinea-pigs, male and female half and half, and body weight 250 1 300g, before administration, 24h is by every guinea pig back both sides unhairing (3cm * 3cm).Laboratory is divided into three groups by plucked Cavia porcellus, tested medicine group, blank group (giving normal saline) and positive controls (1%2,4 one nitrochlorobenzol), 10 every group (each 5 of male and female).Sensitization contact: No. one plant essential oil composition 1.0ml is applied to animal unhairing district, left side and covers with one deck cellophane and two-layer gauze, then uses nonirritant immobilization with adhesive tape, continues 6h.The 7th day and the 14th day in kind respectively repeats sensitization once.Blank group and positive controls give respectively relative medicine with method and carry out sensitization contact.Excite contact: 14d is applied to unhairing district, guinea pig back right side by relative medicine 1.0ml after last administration, after 6h, removing medicine is observable, then in 24,48,72h observes skin allergy situation again.All there is not the serious systemic anaphylaxises such as erythema, edema, asthma, astasia or shock in the tested medicine group of result and blank group, without sensitization.All there is obvious poisoning erythema edema in positive controls, reflection meansigma methods is 4, and sensitization rate 100% is extremely sensitivity.Experimental result show plant essential oil composition to Cavia porcellus without skin allergy, very little on body immune system impact.
Through skin acute toxicity, irritation test and skin anaphylactic test, result show No. two plant essential oil compositions the same with plant essential oil composition to rabbit without obvious acute toxic reaction, to rabbit intact skin group and the equal nonirritant of damaged skin group, to Cavia porcellus without skin allergy.Illustrating that this preparation toxicity is very little, is the good external essence oil of a kind of safety.
The angst resistance effect research of No. 6 plant essential oil compositions of embodiment
Experiment material:
A plant essential oil composition is by oneself preparation.Laboratory animal Kunming kind white mice, male, body weight 18-22g, is provided by Guangdong Province's Experimental Animal Center.
Method and result:
The angst resistance effect of a plant essential oil composition.
40 of mices are got in the experiment of mice forced swimming, with 10% sodium sulfide (Na
2s), at mice buttocks back depilation 1 * lcm, be divided at random matched group (normal saline), (0.4ml is equivalent to crude drug 6.67gkg to a tested combined plant quintessence oil high dose
-1), (0.2ml is equivalent to crude drug 3.33gkg to middle dosage
-1) and low dosage (0.1ml is equivalent to crude drug 1.67gkg
-1) totally 4 groups, 10 every group, at mice buttocks back depilation district coating medicine-feeding 1 * lcm
2only
-1.Every day 2 times, continuously 7d.The front 8 h fasting of last administration, after administration, 1 h tests.Before experiment, (the 6th day) mice training swimming 15 min, test after 24 h: glass jar (high 20cm, the diameter 14cm) water temperature of mice being put into the depth of water 10 cm is controlled 25 ℃, 1, every cylinder.Put into after water adapts to 2 minutes and start to observe 4 minutes, record the accumulation dead time (stop struggling, or be floating state, only have the persistent period of tiny limb motion to keep head to keep afloat) of mice in 4 minutes.
Table 1, quintessence oil are on forcing the impact of mice swimming test
| Group | Administration | Dosage/gkg -1 | The t(dead time) s |
| Normal saline | Put on the skin outward | ? | 30.5±8.0 |
| Quintessence oil low dosage | Put on the skin outward | 1.67 | 48.8±27.1 * |
| Dosage in quintessence oil | Put on the skin outward | 3.33 | 63.6±30.2 * |
| Quintessence oil high dose | Put on the skin outward | 6.67 | 70.3±31.5 * |
*P<0.05
From table 1 result, show, with matched group comparison, No. one the basic, normal, high dosage of plant essential oil composition all has good angst resistance effect (P<0.05).
Tail suspension test is got 40 of mices, with 10% sodium sulfide (Na
2s), at mice buttocks back depilation 1 * lcm, be divided at random matched group (normal saline), (0.4ml is equivalent to crude drug 6.67gkg to a tested plant essential oil composition high dose
-1), (0.2ml is equivalent to crude drug 3.33gkg to middle dosage
-1) and low dosage (0.1ml is equivalent to crude drug 1.67gkg
-1) totally 4 groups, 10 every group, at mice buttocks back depilation district coating medicine-feeding 1 * lcm
2only
-1.Every day 2 times, continuous 7 d.The front 8 h fasting of last administration, after administration, 1 h detects.By mouse tail l 13m place with immobilization with adhesive tape in stand edge, hung upside down in case (30cm * 25cm * 15 cm), head, from approximately 5 cm at the bottom of case, demonstrates mice activity curve by BL420E+ biological function experimental system software.The dead time of mice in observed and recorded 6 minutes.
Table 2, the impact of quintessence oil on mouse tail suspension test
| Group | Administration | Dosage/gkg -1 | The t(dead time) s |
| Normal saline | Put on the skin outward | ? | 50.8±23.3 |
| Quintessence oil low dosage | Put on the skin outward | 1.67 | 60.3±20.3 * |
| Dosage in quintessence oil | Put on the skin outward | 3.33 | 66.6±26.1 * |
| Quintessence oil high dose | Put on the skin outward | 6.67 | 60.1±28.2 * |
*P<0.05
From table 2 result, show all there is good angst resistance effect (P<0.05) with basic, normal, high dosage of plant essential oil composition of matched group comparison.
40 of mices are got in the experiment of mice autonomic activities, with 10% sodium sulfide (Na
2s), at mice buttocks back depilation 1 * lcm, be divided at random matched group (normal saline), (0.4ml is equivalent to crude drug 6.67gkg to a tested plant essential oil composition high dose
-1), (0.2ml is equivalent to crude drug 3.33gkg to middle dosage
-1) and low dosage (0.1ml is equivalent to crude drug 1.67gkg
-1) totally 4 groups, 10 every group, at mice buttocks back depilation district coating medicine-feeding 1 * lcm
2only
-1.Every day 2 times, continuous 7 d.1 h after last administration, puts mice in people's autonomic activities instrument and measures the autonomic activities in 5 minutes.
Table 3, the impact of quintessence oil on the test of mice autonomic activities
| Group | Administration | Dosage/gkg -1 | Autonomic activities number of times (inferior/5 minute) |
| Normal saline | Put on the skin outward | ? | 610.5±160.2 |
| Quintessence oil low dosage | Put on the skin outward | 1.67 | 587.0±130.1 |
| Dosage in quintessence oil | Put on the skin outward | 3.33 | 542.1±122.8 * |
| Quintessence oil high dose | Put on the skin outward | 6.67 | 553.6±134.0 * |
*P<0.05
From table 3 result, show, with in plant essential oil composition of matched group comparison and high dose can reduce the autonomic activities number of times of mice, there is good angst resistance effect.
The angst resistance effect research of 7 No. two plant essential oil compositions of embodiment
Experiment material:
No. two plant essential oil compositions are by oneself preparation.Laboratory animal Kunming kind white mice, male, body weight 18-22g, is provided by Guangdong Province's Experimental Animal Center.
Method and result:
The angst resistance effect of No. two plant essential oil compositions.
40 of mices are got in the experiment of mice forced swimming, with 10% sodium sulfide (Na
2s), at mice buttocks back depilation 1 * lcm, be divided at random matched group (normal saline), (1/3ml is equivalent to crude drug 6.67gkg to tested No. two combined plant quintessence oil high doses
-1), (1/6ml is equivalent to crude drug 3.33gkg to middle dosage
-1) and low dosage (1/12ml is equivalent to crude drug 1.67gkg
-1) totally 4 groups, 10 every group, at mice buttocks back depilation district coating medicine-feeding 1 * lcm
2only
-1.Every day 2 times, continuously 7d.The front 8 h fasting of last administration, after administration, 1 h tests.Before experiment, (the 6th day) mice training swimming 15 min, test after 24 h: glass jar (high 20cm, the diameter 14cm) water temperature of mice being put into the depth of water 10 cm is controlled 25 ℃, 1, every cylinder.Put into after water adapts to 2 minutes and start to observe 4 minutes, record the accumulation dead time (stop struggling, or be floating state, only have the persistent period of tiny limb motion to keep head to keep afloat) of mice in 4 minutes.
Table 4, quintessence oil are on forcing the impact of mice swimming test
| Group | Administration | Dosage/gkg -1 | The t(dead time) s |
| Normal saline | Put on the skin outward | ? | 28.5±15.0 |
| Quintessence oil low dosage | Put on the skin outward | 1.67 | 45.6±26.1 * |
| Dosage in quintessence oil | Put on the skin outward | 3.33 | 64.6±28.2 * |
| Quintessence oil high dose | Put on the skin outward | 6.67 | 69.2±31.0 * |
*P<0.05
From table 4 result, show, with matched group comparison, No. two the basic, normal, high dosage of plant essential oil composition all has good angst resistance effect (P<0.05).
Tail suspension test is got 40 of mices, with 10% sodium sulfide (Na
2s), at mice buttocks back depilation 1 * lcm, be divided at random matched group (normal saline), (1/3ml is equivalent to crude drug 6.67gkg to tested No. two combined plant quintessence oil high doses
-1), (1/6ml is equivalent to crude drug 3.33gkg to middle dosage
-1) and low dosage (1/12ml is equivalent to crude drug 1.67gkg
-1) totally 4 groups, 10 every group, at mice buttocks back depilation district coating medicine-feeding 1 * lcm
2only
-1.Every day 2 times, continuous 7 d.The front 8 h fasting of last administration, after administration, 1 h detects.By mouse tail l 13m place with immobilization with adhesive tape in stand edge, hung upside down in case (30cm * 25cm * 15 cm), head, from approximately 5 cm at the bottom of case, demonstrates mice activity curve by BL420E+ biological function experimental system software.The dead time of mice in observed and recorded 6 minutes.
Table 5, the impact of quintessence oil on mouse tail suspension test
| Group | Administration | Dosage/gkg -1 | The t(dead time) s |
| Normal saline | Put on the skin outward | ? | 48.7±22.2 |
| Quintessence oil low dosage | Put on the skin outward | 1.67 | 61.2±21.3 * |
| Dosage in quintessence oil | Put on the skin outward | 3.33 | 63.4±24.2 * |
| Quintessence oil high dose | Put on the skin outward | 6.67 | 62.2±29.2 * |
*P<0.05
From table 5 result, show all there is good angst resistance effect (P<0.05) with No. two basic, normal, high dosage of plant essential oil composition of matched group comparison.
40 of mices are got in the experiment of mice autonomic activities, with 10% sodium sulfide (Na
2s), at mice buttocks back depilation 1 * lcm, be divided at random matched group (normal saline), (1/3ml is equivalent to crude drug 6.67gkg to tested No. two combined plant quintessence oil high doses
-1), (1/6ml is equivalent to crude drug 3.33gkg to middle dosage
-1) and low dosage (1/12ml is equivalent to crude drug 1.67gkg
-1) totally 4 groups, 10 every group, at mice buttocks back depilation district coating medicine-feeding 1 * lcm
2only
-1.Every day 2 times, continuous 7 d.1 h after last administration, puts mice in people's autonomic activities instrument and measures the autonomic activities in 5 minutes.
Table 6, the impact of quintessence oil on the test of mice autonomic activities
| Group | Administration | Dosage/gkg -1 | Autonomic activities number of times (inferior/5 minute) |
| Normal saline | Put on the skin outward | ? | 622.3±158.2 |
| Quintessence oil low dosage | Put on the skin outward | 1.67 | 600.0±140.1 |
| Dosage in quintessence oil | Put on the skin outward | 3.33 | 538.1±121.3 * |
| Quintessence oil high dose | Put on the skin outward | 6.67 | 535.6±131.8 * |
*P<0.05
From table 6 result, show, with in No. two plant essential oil compositions of matched group comparison and high dose can reduce the autonomic activities number of times of mice, there is good angst resistance effect.
Claims (1)
1. a plant essential oil composition, is characterized in that being comprised of following component and percent by volume:
Cassia twig oil 20-40%,
Resina Cupressi 20-40%,
Chinese juniper oil 20-40%,
B component quintessence oil 5-20%;
Described B component quintessence oil is the supercritical extract of following weight parts component:
20 parts of 10 parts of Fructus Foeniculi, 10 parts of Fructus Schisandrae Chinensis, 10 parts of Radix Aucklandiae (Radix Vladimiriae), 5 parts of Rhizoma Atractylodis, 10 parts of Radix Curcumaes, 10 parts of Flos Chrysanthemi Indicis, 20 parts of Herba Pogostemonis, 5 parts, Lignum Santali Albi, 10 parts of Rhizoma Chuanxiongs, 10 parts of Radix Salviae Miltiorrhizaes, 20 parts of Semen Cassiaes, 5 parts of Olibanums, 5 parts of Myrrhas, 5 parts of Lignum Aquilariae Resinatum and Rhizoma Cyperis; The supercritical extraction method of described B component quintessence oil is: raw material is pulverized, and crosses 40-60 mesh sieve, coordinates by weight and mixes; Pack extraction kettle into, sealing, the parameters of debugging extraction kettle, separating still, heating apparatus and refrigerating plant are normally worked, and open compressor pump and are pressurised into 21-22Mpa, and pumping liquid CO2 extracts plants essential oil, resolves extract from separating still, obtains B component quintessence oil.
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