CN103250594A - Degradable seedling-raising pot preventing and treating root-knot nematodes and preparation method thereof - Google Patents
Degradable seedling-raising pot preventing and treating root-knot nematodes and preparation method thereof Download PDFInfo
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- CN103250594A CN103250594A CN2013101811659A CN201310181165A CN103250594A CN 103250594 A CN103250594 A CN 103250594A CN 2013101811659 A CN2013101811659 A CN 2013101811659A CN 201310181165 A CN201310181165 A CN 201310181165A CN 103250594 A CN103250594 A CN 103250594A
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Abstract
The invention discloses a degradable seedling-raising pot preventing and treating root-knot nematodes and a preparation method thereof and belongs to a cultivating container. The degradable seedling-raising pot is applied to the agriculture pest and disease green prevention and control field. The main ingredients of the seedling-raising pot comprise 5-10% of paecilomyces lilacinus solid state fermentation products, 10-20% of diatomite, 5-15% of nutrient substance, 30-40% of crop straw, 30-40% of clayed soil and 1-1.5% of sodium carboxymethylcellulose, and the components are manufactured according to specific procedures. According to the degradable seedling-raising pot, the paecilomyces lilacinus solid state fermentation products are added to the manufacturing raw materials of the seedling-raising pot, so paecilomyces lilacinus can be effectively protected, shelf lives and a lasting period of a product are prolonged, utilization efficiency of the paecilomyces lilacinus is improved, and plants can be protected in a three-dimensional and all-round mode.
Description
Technical field
The present invention relates to a kind of cultivating container, particularly a kind of application Paecilomyces lilacinus
(Paecilomyces lilacinus)Degradable pot for growing seedlings of control root-knot nematode and preparation method thereof.
Background technology
Root-knot nematode
(Meloidogyne spp.)Be to endanger the heaviest, one of the widest plant pathogeny line insect that distributes, the economic loss that whole world every year is caused by root-knot nematode is up to more than 1,000 hundred million dollars.
At present, the main means of control root-knot nematode are two kinds of chemical control, biological controls, and the used toxicity of pesticide of chemical control is big, seriously polluted, use the pesticide resistance that easily increases pathogenic nematode continuously, can not satisfy the modern agricultural development demand gradually.The biological control aspect, the biocontrol fungicide that with the Paecilomyces lilacinus is main component has carried out using widely in the control of root-knot nematode, but there are some shortcomings in these microbial inoculums, greatly limited the application of Paecilomyces lilacinus, mainly show: 1. existing microbial inoculum mainly exists with granule, pulvis, bacterium piece form, and shelf life is short; In the practice, be vulnerable to the soil environment influence, be difficult for forming and stablize flora, control efficiency instability.2. in use; the Paecilomyces lilacinus microbial inoculum modes of spreading manuer in holes that adopt more; be that CN201110176515.3 discloses a kind of intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus and preparation method thereof that contains as the patent No.; the bacterium piece is imbedded the root system of plant position; its sphere of action is limited; can not be three-dimensional, the root system of omnibearing protection plant, control efficiency is had a greatly reduced quality.
Summary of the invention
The present invention has overcome shortcoming of the prior art, a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode is provided, its advantage is embodied in: 1. Paecilomyces lilacinus solid state fermentation product is added pot for growing seedlings and make in the raw material, Paecilomyces lilacinus is effectively protected, the microbial inoculum activity is affected by environment hardly, has prolonged the shelf life of product; 2. surrounding soil is survived, bred, slowly is discharged into to Paecilomyces lilacinus in the microenvironment of pot for growing seedlings, forms and stablize flora, and the activity of Paecilomyces lilacinus is not vulnerable to the soil environment influence under the protection of pot for growing seedlings, improved its lasting period; 3. contain Paecilomyces lilacinus in the pot for growing seedlings alms bowl body, it is more even that Paecilomyces lilacinus is distributed, and three-dimensional, the comprehensive cover crop root system of energy has farthest been brought into play its effect; 4. the production of pot for growing seedlings, transportation and use are all convenient and simple, are beneficial to large-scale promotion.
Technical scheme of the present invention is:
(1) a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode and preparation method thereof is characterized in that, the pot for growing seedlings raw material comprises: the material of control nematode, protection adsorbent, nutriment, carrier mass and binding agent.Contain the material of preventing and treating nematode in the pot for growing seedlings raw material, grow seedlings, diseases prevention is integrated; Pot for growing seedlings adopts degradation material, and seedling can connect pot for growing seedlings to be transplanted together, for plant provides for a long time, three-dimensional, omnibearing protection.
(2) material of above-mentioned (1) described control nematode is Paecilomyces lilacinus solid state fermentation product, and its spore content is 10
8-10
10Cfu/g.The spore content that Paecilomyces lilacinus is higher has guaranteed the effect that pot for growing seedlings is prevented and treated nematode.
(3) above-mentioned (1) described protection adsorbent is diatomite.The diatomite internal surface area is big, and the spongelike structure that possesses can improve the adhesive force of Paecilomyces lilacinus mycelium and spore, effectively avoids the Paecilomyces lilacinus spore to fly ease, and the Paecilomyces lilacinus microbial inoculum is played a protective role.
(4) above-mentioned (1) described nutriment is a kind of of the former powder of amino acid, the former powder of yeast or two kinds and the mixture of wheat bran.Need to consume amino acid in the Paecilomyces lilacinus reproductive process, the former medicine of amino acid, the former powder of yeast are simple and easy to such an extent that can satisfy the demand again.Wheat bran is loose, not only for Paecilomyces lilacinus provides carbon source, also provides the condition of aerobic respiration.
(5) above-mentioned (1) described carrier mass is the mixture of crops straw and soil substance; Wherein, crops straw comprises: one or more mixtures of wheat stalk, maize straw and rice straw; Soil substance is clayed soil.
(6) above-mentioned (1) described binding agent is sodium carboxymethylcellulose.Sodium carboxymethylcellulose has emulsifying dispersivity, it is corrupt to be difficult for, and physiology is harmless, can form full-bodied colloid, solution, characteristics such as adhesion, thickening, figuration, acidproof, salt tolerant arranged, so select for use sodium carboxymethylcellulose as binding agent of the present invention.
(7) above-mentioned each component of (1) described raw material is respectively by mass content percentage: Paecilomyces lilacinus solid state fermentation product 5-10%, diatomite 10-20%, nutriment 5-15%, crops straw 30-40%, clayed soil 30-40%, sodium carboxymethylcellulose 1-1.5%.
Said ratio has ensured effective quantity and the control efficiency of Paecilomyces lilacinus under the prerequisite of saving cost.
(8) above-mentioned (1) described pot for growing seedlings preparation method is as follows: (a) get above-mentioned each component raw material in proportion; (b) Paecilomyces lilacinus solid state fermentation product and diatomite are stirred after; Adding wheat bran again stirs; (c) sodium carboxymethylcellulose is added water to abundant dissolving, pour in (b), stir; (d) in (c), add crops straw, clayed soil successively, stir; (e) nutriment is added water to abundant dissolving, pour in (d), and add an amount of water, stir; (f) the pot for growing seedlings form of utilizing machine extruding (e) to become to be fit to is standby after drying.
Earlier Paecilomyces lilacinus solid state fermentation product and protection adsorbent diatomite are stirred, add nutriment wheat bran again and stir, can make Paecilomyces lilacinus solid state fermentation product obtain protection; After adding water sodium carboxymethylcellulose and nutriment being dissolved fully, carry out stirring and evenly mixing again, can make the distribution of these materials in mixture more even.
Contain Paecilomyces lilacinus solid state fermentation product and nutriment in the pot for growing seedlings alms bowl body, surrounding soil is survived, bred, is discharged into gradually to Paecilomyces lilacinus in the microenvironment of pot for growing seedlings, form and stablize flora, can at utmost bring into play control efficiency, preventing and treating efficient relatively can reach more than 90%.Pot for growing seedlings adopts degradation material, and seedling can connect pot for growing seedlings to be transplanted together, and flora is growth around root system of plant, in the whole plant strain growth phase, need not use nematocide again, saves medicament and cost of labor.
Embodiment
The present invention is further illustrated by embodiment, and embodiment should not be used as limitation of the present invention.
Embodiment 1: get raw material and amount to 500kg, each constituent mass percentage composition is Paecilomyces lilacinus solid state fermentation product 8%, diatomite 10%, nutriment 6%, crops straw 35%, clayed soil 40%, sodium carboxymethylcellulose 1%.Wherein, nutriment is that the former powder of amino acid, the former powder of yeast mix with wheat bran, and crops straw is wheat stalk.
Get Paecilomyces lilacinus solid state fermentation product 40kg, diatomite 50kg stirs, and adds wheat bran 10kg again and stirs; Get sodium carboxymethylcellulose 5kg, add water to abundant dissolving, pour in the said mixture, stir; Add wheat stalk 175kg more successively, clayed soil 200kg stirs and forms even mud; Get the former powder of amino acid, each 10kg of the former powder of yeast, amount to 20kg, add and pour in the above-mentioned mud after water fully dissolves, add an amount of water again, stir; Utilize the machine extrusion modling, standby after drying.
Embodiment 2:
Get raw material and amount to 600kg, each constituent mass percentage composition is Paecilomyces lilacinus solid state fermentation product 6%, diatomite 15%, nutriment 5%, crops straw 40%, clayed soil 32.5%, sodium carboxymethylcellulose 1.5%.Wherein, nutriment is that the former powder of amino acid, wheat bran mix, and crops straw is that maize straw, rice straw mix.
Get Paecilomyces lilacinus solid state fermentation product 36kg, diatomite 90kg stirs; Adding wheat bran 15kg again stirs; Get sodium carboxymethylcellulose 9kg, add water to abundant dissolving, pour in the said mixture, stir; Add stalk mixture 240kg more successively, clayed soil 195kg stirs into even mud; With the former powder 15kg of amino acid, add and pour in the above-mentioned mud after water fully dissolves, add an amount of water, stir; Utilize the machine extrusion modling, standby after drying.
Embodiment 3:
Get raw material and amount to 1000kg, each constituent mass percentage composition is Paecilomyces lilacinus solid state fermentation product 10%, diatomite 20%, nutriment 8.8%, crops straw 30%, clayed soil 30%, sodium carboxymethylcellulose 1.2%.Wherein, nutriment is that the former powder of yeast, wheat bran mix, and crops straw is that maize straw, wheat stalk mix.
Get Paecilomyces lilacinus solid state fermentation product 100kg, diatomite 200kg stirs; After finishing, add wheat bran 44kg and stir; Get sodium carboxymethylcellulose 12kg, add water to abundant dissolving, pour in the said mixture, stir; Add stalk mixture 300kg more successively, clayed soil 300kg stirs; With the former powder 44kg of amino acid, add and pour in the above-mentioned mud after water fully dissolves, add an amount of water, stir; Utilize the machine extrusion modling, standby after drying.
Embodiment 4:
Get raw material and amount to 800kg, each constituent mass percentage composition is Paecilomyces lilacinus solid state fermentation product 5%, diatomite 11%, nutriment 15%, crops straw 32%, clayed soil 35.7%, sodium carboxymethylcellulose 1.3%.Wherein, nutriment is that the former powder of amino acid, the former powder of yeast, wheat bran mix in right amount, and crops straw is that maize straw, wheat stalk, rice straw mix.
Get Paecilomyces lilacinus solid state fermentation product 40kg, diatomite 88kg stirs; After finishing, add wheat bran 40kg and stir; Get sodium carboxymethylcellulose 10.4kg, add water to abundant dissolving, pour in the said mixture, stir; Add stalk mixture 256kg more successively, clayed soil 285.6kg stirs; Mixture 80kg with the former powder of amino acid and the former powder of yeast adds water and fully dissolves, and pours in the above-mentioned mud, adds an amount of water, stirs; Utilize the machine extrusion modling, standby after drying.
Embodiment 5:
Adopt the degradable pot for growing seedlings that has control root-knot nematode function among the embodiment 1-4 to cultivate cucumber seedling, wherein, the soil of growing seedlings does not add Paecilomyces lilacinus, is called to handle 1, handles 2, handles 3, handles 4.Adopt common degradable pot for growing seedlings to cultivate cucumber seedling, wherein, add Paecilomyces lilacinus in the soil of growing seedlings, and the content of Paecilomyces lilacinus equates with the content of the interior Paecilomyces lilacinus of single pot for growing seedlings alms bowl body among the embodiment 3 in the single pot for growing seedlings soil, is called and handles 5.It is identical to handle among the 1-5 pot for growing seedlings specification, and the content of soil in the pot for growing seedlings, weight, volume are basic identical.Adopt the seedbed to cultivate cucumber seedling for handling 6.Result of the test sees the following form.
The seedling raise period of handling 1-5 is 35-40 days, subsequently with cucumber seedling together with the pot for growing seedlings transplant planting in facility plastic greenhouse.The direct field planting of cucumber seedling of processing 6 is in facility plastic greenhouse.The cultivated area of handling 1-6 is 0.2 mu.Booth is positioned at booth base, Tu Jing village, prosperous town, northwest, Haidian District, Beijing City, comes into operation 13 years in the canopy district, and root-knot nematode is taken place serious.
In the cucumber whole life, repeatedly get the root of different disposal plant and weigh Paecilomyces lilacinus to the control efficiency of root-knot nematode.During sampling, take the cucumber root of handling 1-6 at random respectively, observe, and add up according to the root-knot nematode grade scale, measure its root knot generation progression, record data, grade scale is specific as follows:
0 grade, no root knot;
1 grade, have the root knot radical account for whole 0 ~ 25%;
3 grades, have the root knot radical account for whole 26 ~ 50%;
5 grades, have the root knot radical account for whole 51 ~ 75%;
7 grades, have the root knot radical account for whole 76 ~ 100%.
Wherein,
Root knot index %=∑ (plant at different levels are counted ⅹ level value) ⅹ 100%/(investigate total strain and count the highest value of ⅹ); Control efficiency=(control group root knot index-processed group root knot index) ⅹ 100%/control group root knot index.
The control efficiency statistics is as follows:
From last table result as can be seen, use pot for growing seedlings of the present invention to cultivate cucumber seedling (handling 1-4), in the cucumber whole growth cycle root-knot nematode harm is had good control action, Paecilomyces lilacinus content is more high in the pot for growing seedlings, its control efficiency more good (handling 3, up to 95%).Paecilomyces lilacinus is evenly sneaked in the soil of growing seedlings (handling 5), the control efficiency of root-knot nematode significantly is lower than will add pot for growing seedlings with the amount Paecilomyces lilacinus and make in the raw material and (handle 3).Explanation adds pot for growing seedlings with Paecilomyces lilacinus to be made in the raw material, has improved the utilization ratio of Paecilomyces lilacinus.
The soil specimen of cucumber plant rhizosphere among the regular acquisition process 1-6 of difference, the whole growth cycle is gathered soil sample altogether 4 times, measures the content of Paecilomyces lilacinus in institute's soil sampling.Result such as following table.
| Paecilomyces lilacinus spore content cfu/g in the soil sample | Handle 1 | Handle 2 | Handle 3 | Handle 4 | Handle 5 | Handle 6 |
| Seedling stage | 2.6*10 7 | 7.3*10 7 | 1.1*10 8 | 5.0*10 7 | 8.6*10 5 | — |
| Flowering stage | 3.1*10 6 | 4.5*10 6 | 8.5*10 6 | 6.4*10 6 | 1.6*10 4 | — |
| The knot melon phase | 1.3*10 6 | 2.3*10 6 | 3.4*10 6 | 4.5*10 6 | 5.2*10 2 | — |
| Uproot plants after their edible portions have been harvested the phase | 5.3*10 5 | 4.8*10 5 | 7.5*10 5 | 8.6*10 5 | 0 | — |
The result shows, all detect the existence of Paecilomyces lilacinus in the cucumber plant rhizosphere soil that uses pot for growing seedlings of the present invention (handling 1-4) to cultivate, and Paecilomyces lilacinus content keeps dynamic equilibrium in the crop whole growth cycle.Adopt common pot for growing seedlings, in the soil of growing seedlings, sneak into the processing (handle 5) of Paecilomyces lilacinus, detect the existence of Paecilomyces lilacinus in earlier stage at cucumber growth, grow after 2 months, just can not detect the existence of Paecilomyces lilacinus in the rhizosphere soil.Illustrate, adopt pot for growing seedlings of the present invention to grow seedlings and to improve the lasting period of Paecilomyces lilacinus in soil.In the processing of growing seedlings in the seedbed, (handle 6), do not detect the existence of Paecilomyces lilacinus.
Claims (8)
1. prevent and treat degradable pot for growing seedlings of root-knot nematode and preparation method thereof for one kind, it is characterized in that, the pot for growing seedlings raw material comprises: the material of control nematode, protection adsorbent, nutriment, carrier mass and binding agent.
2. a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode according to claim 1 and preparation method thereof is characterized in that, the material of described control nematode is Paecilomyces lilacinus
(Paecilomyces lilacinus)Solid state fermentation product, its spore content are 10
8-10
10Cfu/g.
3. a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode according to claim 1 and preparation method thereof is characterized in that, described protection adsorbent is diatomite.
4. a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode according to claim 1 and preparation method thereof is characterized in that, described nutriment is a kind of of the former powder of amino acid, the former powder of yeast or two kinds and the mixture of wheat bran.
5. a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode according to claim 1 and preparation method thereof is characterized in that, described carrier mass is the mixture of crops straw and soil substance; Wherein, crops straw comprises: one or more mixtures of wheat stalk, maize straw and rice straw; Soil substance is clayed soil.
6. a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode according to claim 1 and preparation method thereof is characterized in that, described binding agent is sodium carboxymethylcellulose.
7. a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode according to claim 1 and preparation method thereof, it is characterized in that, each component of raw material is respectively by mass content percentage: Paecilomyces lilacinus solid state fermentation product 5-10%, diatomite 10-20%, nutriment 5-15%, crops straw 30-40%, clayed soil 30-40%, sodium carboxymethylcellulose 1-1.5%.
8. a kind of degradable pot for growing seedlings of preventing and treating root-knot nematode according to claim 1 and preparation method thereof is characterized in that, the pot for growing seedlings preparation method is as follows: (a) get above-mentioned each component raw material in proportion; (b) Paecilomyces lilacinus solid state fermentation product and diatomite are stirred after; Adding wheat bran again stirs; (c) sodium carboxymethylcellulose is added water to abundant dissolving, pour in (b), stir; (d) in (c), add crops straw, clayed soil successively, stir; (e) nutriment is added water to abundant dissolving, pour in (d), and add an amount of water, stir; (f) the pot for growing seedlings form of utilizing machine extruding (e) to become to be fit to is standby after drying.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103461389A (en) * | 2013-09-13 | 2013-12-25 | 成都天星农业科技有限公司 | Compound microorganism bacteria agent capable of preventing root-knot nematode and preparation method thereof |
| CN103787743A (en) * | 2014-01-09 | 2014-05-14 | 东北农业大学 | Biological soil conditioning agent formula applicable to improving crop continuous cropping soil |
| CN104025945A (en) * | 2014-06-26 | 2014-09-10 | 杨宏勃 | Degradable seedling culture pot and production method thereof |
| CN104823753A (en) * | 2015-05-29 | 2015-08-12 | 东营市康森秸秆制品有限公司 | Degradable seedling culturing bowl prepared with straw and preparation method thereof |
| CN104956948A (en) * | 2015-06-14 | 2015-10-07 | 卞洪锋 | Preparation method for breeding cup and breeding cup prepared with preparation method |
| CN105875168A (en) * | 2016-05-24 | 2016-08-24 | 福建省农业科学院亚热带农业研究所 | Continuous-cropping high-yield cultivation method of okra |
| CN107306692A (en) * | 2017-08-02 | 2017-11-03 | 合肥百绿盛农业科技有限公司 | One kind is exempted to heel in insect prevention pot for growing seedlings |
| CN112940946A (en) * | 2021-01-27 | 2021-06-11 | 贵州大学 | Method for preparing paecilomyces lilacinus from marigold straws to prevent and control tobacco root-knot nematodes |
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| CN103026920A (en) * | 2012-12-29 | 2013-04-10 | 山东坤基沃业生物科技股份有限公司 | Fully degradable seedling-growing integrated nutritional bowl and production method thereof |
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| CN103461389A (en) * | 2013-09-13 | 2013-12-25 | 成都天星农业科技有限公司 | Compound microorganism bacteria agent capable of preventing root-knot nematode and preparation method thereof |
| CN103461389B (en) * | 2013-09-13 | 2015-02-04 | 成都天星农业科技有限公司 | Compound microorganism bacteria agent capable of preventing root-knot nematode and preparation method thereof |
| CN103787743A (en) * | 2014-01-09 | 2014-05-14 | 东北农业大学 | Biological soil conditioning agent formula applicable to improving crop continuous cropping soil |
| CN103787743B (en) * | 2014-01-09 | 2016-04-06 | 东北农业大学 | A kind of biological soil conditioning agent compound method being applicable to vegetable continuous cropping soil improvement |
| CN104025945A (en) * | 2014-06-26 | 2014-09-10 | 杨宏勃 | Degradable seedling culture pot and production method thereof |
| CN104025945B (en) * | 2014-06-26 | 2016-03-30 | 杨宏勃 | A kind of degradable pot for growing seedlings and production method thereof |
| CN104823753A (en) * | 2015-05-29 | 2015-08-12 | 东营市康森秸秆制品有限公司 | Degradable seedling culturing bowl prepared with straw and preparation method thereof |
| CN104956948A (en) * | 2015-06-14 | 2015-10-07 | 卞洪锋 | Preparation method for breeding cup and breeding cup prepared with preparation method |
| CN105875168A (en) * | 2016-05-24 | 2016-08-24 | 福建省农业科学院亚热带农业研究所 | Continuous-cropping high-yield cultivation method of okra |
| CN107306692A (en) * | 2017-08-02 | 2017-11-03 | 合肥百绿盛农业科技有限公司 | One kind is exempted to heel in insect prevention pot for growing seedlings |
| CN112940946A (en) * | 2021-01-27 | 2021-06-11 | 贵州大学 | Method for preparing paecilomyces lilacinus from marigold straws to prevent and control tobacco root-knot nematodes |
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Owner name: BEIJING ECOMAN BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER NAME: BEIJING YIKEMAN BIOLOGICAL TECHNOLOGY CO., LTD. |
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Address after: 100085, Beijing, Haidian District on the road No. 26, Zhongguancun venture building, Room 518 Patentee after: BEIJING ECOMAN BIOTECHNOLOGY CO., LTD. Address before: 100085, Beijing, Haidian District on the road No. 26, Zhongguancun venture building, Room 518 Patentee before: Beijing Ecoman Biotech Co., Ltd. |