CN103196975A - Detection apparatus and detection method of medical relative oxidation reduction potential - Google Patents
Detection apparatus and detection method of medical relative oxidation reduction potential Download PDFInfo
- Publication number
- CN103196975A CN103196975A CN2013100697514A CN201310069751A CN103196975A CN 103196975 A CN103196975 A CN 103196975A CN 2013100697514 A CN2013100697514 A CN 2013100697514A CN 201310069751 A CN201310069751 A CN 201310069751A CN 103196975 A CN103196975 A CN 103196975A
- Authority
- CN
- China
- Prior art keywords
- reduction potential
- oxidation
- detector tube
- reference liquid
- valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a detection apparatus and a detection method of medical relative oxidation reduction potential. The detection apparatus comprises a detecting tube and an elastic sealing plug mounted at a top opening of the detecting tube body, wherein the elastic sealing plug is provided with a first through hole and a second through hole, a detecting electrode passes through the first through hole, a communicating fine pipe passes through the second through hole, and a valve is mounted at one end of the communicating fine pipe located outside the detecting tube. The detection method comprises: (1) vacuumizing the detecting tube through the communicating fine pipe, adding reference liquid into the detecting tube, closing the valve, and detecting an oxidation reduction potential of the reference liquid; (2) opening the valve, adding to-be-detected liquid into the reference liquid, closing the valve, uniformly mixing to obtain a mixed liquid, and detecting an oxidation reduction potential of the mixed liquid; and (3) calculating a difference between the oxidation reduction potentials of the mixed liquid and the reference liquid to obtain an oxidation reduction potential of the to-be-detected liquid relative to the reference liquid.
Description
Technical field
The invention belongs to the clinical medicine detection range, relate in particular to a kind of pick-up unit and detection method of medical relative oxidation-reduction potential.
Background technology
(Oxidation Reduction Potential is a composite target of medium (comprising soil, natural water, nutrient culture media etc.) environmental baseline ORP) to oxidation-reduction potential, and it has characterized the relative extent of medium oxidisability or reductibility.
For a water body, often there is multiple oxidation-reduction pair, constitute complicated redox system.The oxidation-reduction potential of water body then is the synthesis result of multiple oxidation material and reducing substances generation redox reaction, is used for reflecting macroscopical oxidation-reducibility that all substances show in the aqueous solution.This index helps to understand the electrochemical characteristic of water body, the character of analyzing water body.
The measurement mechanism of oxidation-reduction potential is made up of ORP electrode and potential difference meter usually, and the ORP electrode is a kind of electrode that can carry out Electron absorption or release on its sensitive layer surface, and this sensitive layer is a kind of inert metal, makes of platinum and Jin Lai usually.Contrast electrode is silver/silver chloride electrode or saturated calomel electrode.
The traditional measurement oxidation-reduction potential generally adopts the direct determination method of platinum electrode or depolarization determination method etc.The direct determination method of platinum electrode is about to platinum electrode and contrast electrode and directly inserts in the medium and measure.But when measuring weak equilibrium system, because platinum electrode and nisi inertia, its surface can form oxide film or adsorb other materials, thereby influences the electron exchange speed of each oxidation-reduction pair on platinum electrode, makes that the foundation of equilibrium potential is very slow.
Adopt processing such as beveled electrodes so traditional assay method before each mensuration more, with the stability of the mensuration state that guarantees electrode.And traditional open, the semi open model assay method because the influence of extraneous oxygen etc., inevitably will influence the stability of measurement result.
But also not having special-purpose pick-up unit when measuring, generally is to select for use the ready-made instrument in laboratory to assemble the acquisition pick-up unit according to the requirement of correlation method.Complex structure not only, complex steps, and often run into the problem that poorly sealed or water sample can not proper flow can't the real conditions of simulated water sample in actual environment, and polarization effect very easily takes place electrode, produces unnecessary error.
Notification number is that the Chinese patent literature of CN202083664U discloses a kind of anti-electrode polarization effect oxidation-reduction potential detection bottle, the bottle top end opening also is equipped with gland bonnet, bottle side wall is provided with measurement scale line, bottle internal upper part 1/5th places are provided with screen pack, and 1/5th places, bottom are provided with separation net.Gland bonnet is provided with 2～5 holes, is used for laying temperature sensor and detecting electrode.
For the application of medical domain, because the human internal environment is the liquid environment of sealing, and existing oxidation reduction potential determination method complicated operation, electrode before measuring is handled loaded down with trivial details, stable electrode state is difficult for holding, and accidental error is bigger, and the clinical medical timely monitoring of incompatibility is used.
Summary of the invention
The invention provides a kind of pick-up unit of medical relative oxidation-reduction potential, utilize this device under the state that sealing is flowed, the oxidation-reduction potential of solution to be measured, avoid the external environment influence, increased the stability of measuring.
A kind of pick-up unit of medical relative oxidation-reduction potential, comprise detector tube and the resilient seal plug that is installed in detector tube body top end opening, described resilient seal is provided with first through hole and second through hole beyond the Great Wall, be penetrated with detecting electrode in first through hole, be penetrated with the connection tubule in second through hole, the connection tubule is positioned at the outer end of detector tube valve is installed.
Detecting electrode and first through hole, be communicated with tubule and second through hole is sealed and matched respectively, guarantee the sealed environment in the detector tube.
Described valve is preferably three-way cock, and a port of this three-way cock links to each other with the connection tubule, and all the other ports are connected with feed tube.Usually, select syringe to add solution in detector tube, therefore, the caliber of feed tube should adapt with syringe.The feed tube of two ports is respectively applied to add reference liquid and solution to be measured, avoids syringe to pollute mutually.
According to concrete needs, can select to have the switch of different number ports, but the number of port is at least two.Usually, three-way cock can satisfy basic detection requirement.
During use, detecting electrode should be stretched at the bottom of the pipe of detector tube as far as possible, so use a spot of solution can soak into detecting electrode, save solution to be measured; But detecting electrode can not contact with at the bottom of the pipe of detector tube, in order to avoid detecting electrode is caused damage, influences testing result.
The present invention should provide a kind of method of utilizing described pick-up unit to detect relative oxidation-reduction potential, comprising:
(1) by after being communicated with tubule detector tube being vacuumized, add reference liquid in detector tube, valve-off detects the oxidation-reduction potential of reference liquid;
(2) open valve, in reference liquid, add solution to be measured, valve-off, mixing obtains mixed liquor, detects the oxidation-reduction potential of mixed liquor;
(3) difference of the oxidation-reduction potential of the oxidation-reduction potential of calculating mixed liquor and reference liquid obtains solution to be measured with respect to the oxidation-reduction potential of reference liquid.
Preferably, adopt syringe to vacuumize.Syringe is easy to obtain, and is easy and simple to handle.All detector tube is vacuumized before each the detection, avoid the interior residual air of detector tube to the influence of SOLUTION PROPERTIES, guarantee the accuracy of testing result.
Detected temperatures is preferably 23～25 ℃, more preferably 25 ℃.Approach with room temperature, be easy to control.
The volume ratio of reference liquid and solution to be measured is used the method requirement of measuring electrode according to reality, requires to regulate according to liquid measure.
When detecting oxidation-reduction potential, detector tube placed on the horizontal shaking table slowly run, rotating speed is preferably 20～30r/min, more preferably 20r/min.So can accelerate the reaction velocity of solution and detecting electrode.Rotate and appropriate to the occasion detector tube is vertically placed, in order to avoid influence the liquid level requirement of the mensuration liquid liquid measure in the detector tube.
Pick-up unit of the present invention and method are specially adapted to clinical medicine domain, and the liquid environment in the human body is carried out the detection of relative oxidation-reduction potential, to characterize it with respect to the redox state of reference liquid, carry out the quantification monitoring of vivo oxidation reducing condition.During mensuration, different relatively body fluid should be selected the close reference liquid of physiological property with it for use.
Because blood is the main carrier of carrying various metabolic products, be the important liquid environment of regulating metabolism in the body, the redox state of blood is the redox state of the interior liquid environment of antimer totally.Therefore in the clinical medicine, often select for use blood plasma as detecting sample (with Lactated Ringer'S Solution as reference liquid).Blood plasma has been got rid of the influence of cell component etc., the stability that is conducive to measure, reliability.
Compared with prior art, beneficial effect of the present invention is:
(1) pick-up unit of the present invention is measured the oxidation-reduction potential of solution under the state that sealing is flowed, and has avoided the influence of the factors such as oxygen in the external environment, has increased accuracy and the stability of testing result;
(2) detection method of the present invention utilize reference liquid the relative determination value as quantizating index, the accidental error that has caused owing to detecting electrode is inconsistent when having avoided each measure.
Description of drawings
Fig. 1 is the structural representation of the pick-up unit of the medical relative oxidation-reduction potential of the present invention;
Fig. 2 is the The dynamic monitor result figure of detection method of the present invention to burn patient blood plasma; Horizontal ordinate represents that blood plasma takes from burn back the 0th, 1,3,5,7 day and when leaving hospital of patient.
Embodiment
Below in conjunction with the drawings and specific embodiments technical scheme of the present invention is described in further detail.
The pick-up unit of medical relative oxidation-reduction potential as shown in Figure 1 comprises detector tube 1, is installed in the resilient seal plug 2 of detector tube 1 body top end opening.
During use, detecting electrode 3 is extended at the bottom of the pipe of detector tube 1 as far as possible, guarantee that detecting electrode 3 fully immerses in the solution, also can detect when only small volume of solution being arranged in the detector tube 1.The degree that stretches into of detecting electrode 3 in order to avoid detecting electrode 3 is caused damage, influences testing result not to be advisable with contacting at the bottom of the pipe of detector tube 1.
Usually, adopt syringe that solution is added in the detector tube 1, feed tube 52, feed tube 53 are respectively applied to add reference liquid and solution to be measured, avoid drawing between the syringe of corresponding solution and pollute mutually.
In this embodiment, it is the ORP electrode 31 of HI3131B that detecting electrode 3 adopts Italian Kim Yong-jin model.
When carrying out the detection of relative oxidation-reduction potential, at first adjust ORP electrode 31, be communicated with the relative fixed position of tubule 4 on resilient seal plug 2, again resilient seal plug 2 is installed in the detector tube 1 body top end opening;
Adopt syringe that the air in the detector tube 1 is passed through three-way cock 51 and extract out, add reference liquid from feed tube 52 through connection tubule 4, adopt the residual air in syringe extraction detector tube 1 and the connection tubule 4, close three-way cock 51; Pick-up unit vertically placed on the shaking table slowly run, rotating speed is 20r/min, to accelerate detection rates; Utilize ORP electrode 31 to detect oxidation-reduction potential and the record of reference liquid;
Open three-way cock 51, add solution to be measured from feed tube 53 through being communicated with tubule 4, adopt syringe to extract detector tube 1 and the residual air that is communicated with in the tubule 4 out, close three-way cock 51; Solution to be measured and reference liquid mixing are obtained mixed liquor, detect oxidation-reduction potential and the record of mixed liquor;
The difference of the oxidation-reduction potential of calculating mixed liquor and the oxidation-reduction potential of reference liquid obtains solution to be measured relatively and the oxidation-reduction potential of reference liquid, in order to indicate the redox state of solution to be measured.
Be testing sample with burn patient blood plasma (plasma) below, with potassium permanganate (KMnO
4), vitamin C (Vc) is control sample, Lactated Ringer'S Solution (lactated ringer's solution with identical with blood plasma pH (through adding the medical 5% soda solution modulation of trace), LR) be reference liquid, to accuracy and the stability of pick-up unit of the present invention and detection method.
The acquisition process of burn patient blood plasma is:
Patient temperature control is at about 37-38 ℃, go associated treatment such as antioxidant before the blood sampling in half an hour, and the arteries and veins blood gas analysis inspection of taking action simultaneously, and behind the affirmation Non Apparent Abnormality, adopt negative pressure of vacuum to get blood vessel (BD company product in the stipulated time in the morning, the heparin lithium anti-freezing), get the about 2-3mL of femoral vein blood; The centrifugal 5min of 2500r/min gets supernatant blood plasma under the normal temperature, injects the airtight identical negative pressure of vacuum of another and gets in the blood vessel, stand-by (can-80 degree preserve).
Detection method is described identical with embodiment 1, and detection architecture and testing result are as shown in table 1.
The accuracy test of table 1 detection method of the present invention
By table 1 as seen, compare KMnO with LR solution
4The Δ ORP of LR solution is-231mV to show KMnO relatively
4LR solution is oxidation state relatively, with KMnO
4For the general knowledge of oxygenant conforms to; Compare with LR solution, the Δ ORP of the relative LR solution of Vc is 323mV, show that the relative LR solution of Vc is to go back ortho states, and be that the general knowledge of reductive agent conforms to Vc.Illustrate that detection method of the present invention has the galvanochemistry accuracy.
Adopt pick-up unit and the detection method of embodiment 1, adopt the detection architecture of embodiment 2, under without condition (the instant detection with-80 ℃ of preservation backs of spending the night detected) the relative oxidation-reduction potential of plasma sample to be measured is carried out 10 continuous detecting (detecting under 25 ℃ of room temperatures), testing result sees Table 2.
Table 2 detection method stability test of the present invention
By table 2 as seen, 10 times continuous measurement result has shown less value for coefficient of variation, has shown that assay method has good stable.
Embodiment 4
Adopt pick-up unit and the detection method of embodiment 1, adopt the detection architecture of embodiment 2, the plasma specimen early stage to 23 routine serious burn patients' the course of disease carries out the clinical practice dynamic monitoring, and monitoring result as shown in Figure 2.
As seen from Figure 2, in the early stage plasma specimen clinical practice dynamic monitoring of 23 routine serious burn patients' the course of disease, shown the dynamic change of patient's oxidative stress status: namely change the generation (increase of blood oxygen voltinism) that the ischemical reperfusion injury that causes causes oxidative stress because of shock stage in early days, and behind active treatment, the performance of go down (oxidisability of blood gradually reduces) of oxidative stress.Verified the consistance of this assay method result and clinical pathologic characteristic.The feasibility that has shown the practical application of this assay method.
Claims (8)
1. the pick-up unit of a medical relative oxidation-reduction potential, comprise detector tube (1) and be installed in the resilient seal plug (2) of detector tube (1) body top end opening, described resilient seal plug (2) is provided with first through hole (21) and second through hole (22), be penetrated with detecting electrode (3) in first through hole (21), it is characterized in that, be penetrated with connection tubule (4) in second through hole (22), connection tubule (4) is positioned at the outer end of detector tube (1) valve (5) is installed.
2. pick-up unit as claimed in claim 1 is characterized in that, described valve (5) is three-way cock (51), and a port of this three-way cock (51) links to each other with connection tubule (4), and all the other ports are connected with feed tube (52,53).
3. a method of utilizing claim 1 or 2 described pick-up units to detect relative oxidation-reduction potential is characterized in that, comprising:
(1) by after being communicated with tubule (4) detector tube (1) being vacuumized, add reference liquid in detector tube (1), valve-off (5) detects the oxidation-reduction potential of reference liquid;
(2) open valve (5), in reference liquid, add solution to be measured, valve-off (5), mixing obtains mixed liquor, detects the oxidation-reduction potential of mixed liquor;
(3) difference of the oxidation-reduction potential of the oxidation-reduction potential of calculating mixed liquor and reference liquid obtains solution to be measured with respect to the oxidation-reduction potential of reference liquid.
4. method as claimed in claim 3 is characterized in that, adopts syringe to vacuumize, and extracts the gas of detector tube capacity before each the mensuration out.
5. method as claimed in claim 3 is characterized in that, detected temperatures is 23～25 ℃.
6. method as claimed in claim 5 is characterized in that, detected temperatures is 25 ℃.
7. method as claimed in claim 3 is characterized in that, when detecting oxidation-reduction potential, detector tube is placed on the horizontal shaking table slowly run, and rotating speed is 20～30r/min.
8. method as claimed in claim 7 is characterized in that, when detecting oxidation-reduction potential, detector tube is placed on the horizontal shaking table slowly run, and rotating speed is 20r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310069751.4A CN103196975B (en) | 2013-03-05 | 2013-03-05 | Detection apparatus and detection method of medical relative oxidation reduction potential |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310069751.4A CN103196975B (en) | 2013-03-05 | 2013-03-05 | Detection apparatus and detection method of medical relative oxidation reduction potential |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103196975A true CN103196975A (en) | 2013-07-10 |
| CN103196975B CN103196975B (en) | 2014-11-05 |
Family
ID=48719667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310069751.4A Expired - Fee Related CN103196975B (en) | 2013-03-05 | 2013-03-05 | Detection apparatus and detection method of medical relative oxidation reduction potential |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103196975B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004053613A (en) * | 2002-07-19 | 2004-02-19 | Shotaro Oka | Oxidation-reduction potential measuring device |
| JP2010145380A (en) * | 2008-12-22 | 2010-07-01 | Dkk Toa Corp | Method for inspecting electrode of oxidation-reduction potential measuring apparatus, and standard solution for inspecting electrode of oxidation-reduction potential measuring apparatus |
| CN202083664U (en) * | 2011-01-11 | 2011-12-21 | 南开大学 | Oxidation reduction potential (ORP) detecting bottle capable of preventing electrode polarization effect |
| US20120325685A1 (en) * | 2007-05-18 | 2012-12-27 | Institute For Molecular Medicine, Inc. | Measurement and uses of oxidative stress |
-
2013
- 2013-03-05 CN CN201310069751.4A patent/CN103196975B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004053613A (en) * | 2002-07-19 | 2004-02-19 | Shotaro Oka | Oxidation-reduction potential measuring device |
| US20120325685A1 (en) * | 2007-05-18 | 2012-12-27 | Institute For Molecular Medicine, Inc. | Measurement and uses of oxidative stress |
| JP2010145380A (en) * | 2008-12-22 | 2010-07-01 | Dkk Toa Corp | Method for inspecting electrode of oxidation-reduction potential measuring apparatus, and standard solution for inspecting electrode of oxidation-reduction potential measuring apparatus |
| CN202083664U (en) * | 2011-01-11 | 2011-12-21 | 南开大学 | Oxidation reduction potential (ORP) detecting bottle capable of preventing electrode polarization effect |
Non-Patent Citations (1)
| Title |
|---|
| 苏有勇: "《沼气发酵检测技术》", 31 March 2011, article "氧化还原电位的测定" * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103196975B (en) | 2014-11-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103995139B (en) | The external performance evaluating system of continuous blood sugar monitoring sensor | |
| CN202005754U (en) | Fluorescent optical fiber sensor capable of monitoring dissolved oxygen partial pressure and carbon dioxide partial pressure in blood | |
| CN105372429A (en) | Microfluidic chip for bacterial endotoxin and (1-3)-beta-D-glucan detection based on TAL (tachypleus amebocyte lysate) | |
| US20170191954A1 (en) | Differential carbon dioxide sensor | |
| CN101158676B (en) | Analysis method and device for evaluating blood and crucifixes oxygen carrying and oxygen releasing function thereof | |
| CN105158310B (en) | A kind of micro-fluidic detection chip and its application based on micro-porous electrode | |
| CN103196975B (en) | Detection apparatus and detection method of medical relative oxidation reduction potential | |
| CN108918451B (en) | Real-time detection and dynamic intervention device and test intervention method for cell metabolism | |
| CN102182116B (en) | Preparation and application method of filter paper collecting biological sample | |
| CN105759022A (en) | Gas sensor and detection equipment for percutaneous gas analysis | |
| CN202710509U (en) | Biological reaction vessel | |
| CN203011883U (en) | Chemical sensor | |
| CN104673664A (en) | Blood sample culture device | |
| CN206248606U (en) | A kind of measuring electrode for detecting uric acid | |
| EP2816348A1 (en) | Electrochemical measurement device | |
| CN204734491U (en) | What can be used for lasting blood sugar monitoring has pressure measurement of a wound arteriopuncture needle | |
| CN204807439U (en) | Be applicable to and water funkly be afraid of that oxygen compound carries out ultraviolet cell for fluorescence detection | |
| CN107505244A (en) | A kind of Dispersion Test method suitable for hypotonicity soil | |
| CN203290918U (en) | Light-avoiding blood collecting tube | |
| CN202024944U (en) | Multi-passage blood viscosity measuring device | |
| CN207908428U (en) | Detect original position and the dystopy detection device of different depth soil pH and Eh | |
| CN205749522U (en) | A kind of gas sensor for percutaneous gas analysis | |
| CN201075103Y (en) | Device for measuring non-damnification of membrane potential and membrane resistance of single algae cell | |
| KR20200001379A (en) | Carbon dioxide collection device of food | |
| CN205091292U (en) | Sodium, potassium, biochemical analysis dry plate of chlorion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141105 Termination date: 20160305 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |