CN103191219A - Traditional chinese medicine composition and preparation method thereof - Google Patents
Traditional chinese medicine composition and preparation method thereof Download PDFInfo
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- CN103191219A CN103191219A CN2013101186624A CN201310118662A CN103191219A CN 103191219 A CN103191219 A CN 103191219A CN 2013101186624 A CN2013101186624 A CN 2013101186624A CN 201310118662 A CN201310118662 A CN 201310118662A CN 103191219 A CN103191219 A CN 103191219A
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Abstract
The invention relates to a natural pharmaceutical composition, a preparation method thereof and application thereof in preparation of toxicity reducing and efficacy enhancing drugs for cancer chemotherapy. The natural pharmaceutical composition consists of a decoction concentrated extract of crataegus pinnatifida and aquilaria sinensis, crataegus pinnatifida total flavonoids, a chloroform extract of aquilaria sinensis and a hexane extract of aquilaria sinensis which are in the ratio of (2-5):(5-7):(3-9):(2-4). The composition has an excellent treatment effect on cancer-chemotherapy-related diarrhea and can simultaneously play a synergic role in cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing cancer chemotherapy dependency diarrhoea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic no matter singly with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc. in recent years, the incidence rate of chemotherapy dependency diarrhoea improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of chemotherapy dependency diarrhoea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but at present still unmatchful anticancer disease chemotherapy have the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Fructus Crataegi another name Fructus Pyri Pashiae fruit, Rhododendron simsii Planch. fruit, it is long-pending to help digestion; Change the stasis of blood that stagnates.Cure mainly eating accumulation, dysentery.Lignum Aquilariae Resinatum is the dry stem of thymelaeceae plant Lignum Aquilariae Resinatum, the property of medicine: bitter, sour, puckery, flat, return lung, large intestine channel; The relieving diarrhea with astringents effect is arranged, it is the medicine commonly used of controlling chronic diarrhea, chronic dysentery, proctoptosis, in recent years, Fructus Crataegi and Lignum Aquilariae Resinatum antitumaous effect are confirmed, especially Fructus Crataegi total flavones and Lignum Aquilariae Resinatum hexane extraction thing, and the inventor is through research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, be the compositions of novelty, and proved that it has fabulous curative effect and can play potentiation to cancer chemotherapy simultaneously cancer chemotherapy dependency diarrhoea, has finished the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, by Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing by weight in 2-5: the ratio of 5-7: 3-9: 2-4 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 5: 7: 9: 4.
Wherein, Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Crataegi, Lignum Aquilariae Resinatum, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
The preparation method of Fructus Crataegi total flavones is in the above-mentioned natural drug composition:
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely.
Lignum Aquilariae Resinatum chloroform extract preparation method is in the above-mentioned natural drug composition:
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract.
Lignum Aquilariae Resinatum hexane extraction thing preparation method is in the above-mentioned natural drug composition:
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Crataegi, Lignum Aquilariae Resinatum, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely.
(3) Lignum Aquilariae Resinatum chloroform extract preparation
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract.
(4) Lignum Aquilariae Resinatum hexane extraction thing preparation
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing.
(5) choose Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing in proportion, evenly mix, namely.
The preparation method of above-mentioned natural drug composition, also comprise: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing originally, and by the consumption proportion between definite these components of a large amount of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 2-5: 5-7: 3-9: produced unexpected synergism during 2-4, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio in each component is 5: 7: 9: 4 o'clock especially obvious, this is that the inventor pays a large amount of creative works by keeping punching and obtains on the paper basis of delivering before, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and each component of Lignum Aquilariae Resinatum hexane extraction thing all can obtain by conventional means, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Crataegi, Lignum Aquilariae Resinatum, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
(2) system of Fructus Crataegi total flavones respectively
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely.
(3) Lignum Aquilariae Resinatum chloroform extract preparation
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract.
(4) Lignum Aquilariae Resinatum hexane extraction thing preparation
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract 20mg, Fructus Crataegi total flavones 28mg, Lignum Aquilariae Resinatum chloroform extract 36mg and the Lignum Aquilariae Resinatum hexane extraction thing 16mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract 20mg, Fructus Crataegi total flavones 28mg, Lignum Aquilariae Resinatum chloroform extract 36mg and the Lignum Aquilariae Resinatum hexane extraction thing 16mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 5: 7: 9: 4.The inventor to above-mentioned each composition usage ratio at 2-5: 5-7: 3-9: the compositions that the multiple proportioning combination in the time of between the 2-4 obtains has been carried out and last identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Fructus Crataegi, Lignum Aquilariae Resinatum is purchased in medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Fructus Crataegi total flavones 10g.kg
-1(being equivalent to contain the crude drug amount), D group Lignum Aquilariae Resinatum chloroform extract mixes 10g.kg with Lignum Aquilariae Resinatum hexane extraction thing in 9: 4 ratios
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies chemotherapy dependency diarrhoea model at every day 1 time.B, c, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, the degree of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract group chemotherapy dependency diarrhoea is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing by weight in 2-5: the ratio of 5-7: 3-9: 2-4 is formed
Wherein Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration preparation method of extract is:
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Crataegi, Lignum Aquilariae Resinatum, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, be Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract;
Wherein the preparation method of Fructus Crataegi total flavones is:
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely;
Wherein the preparation method from Radix Aucklandiae chloroform extract is:
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract;
Wherein the preparation method of Lignum Aquilariae Resinatum hexane extraction thing is:
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 5: 7: 9: 4.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Crataegi, Lignum Aquilariae Resinatum, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, be Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract;
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely;
(3) preparation of Lignum Aquilariae Resinatum chloroform extract
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract;
(4) preparation of Lignum Aquilariae Resinatum hexane extraction thing
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing;
(5) choose Fructus Crataegi Lignum Aquilariae Resinatum water boiling concentration extract, Fructus Crataegi total flavones, Lignum Aquilariae Resinatum chloroform extract in proportion and evenly mix with Lignum Aquilariae Resinatum hexane extraction thing, namely.
4. the preparation method of natural drug composition as claimed in claim 3, also comprise: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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| CN102145159A (en) * | 2011-03-31 | 2011-08-10 | 青岛大学 | Ginger and elecampane composition, preparation method of ginger and elecampane composition and use of ginger and elecampane composition for preparing toxicity-reducing efficacy-improving medicine in cancer radiotherapy and chemotherapy |
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| CN102145159A (en) * | 2011-03-31 | 2011-08-10 | 青岛大学 | Ginger and elecampane composition, preparation method of ginger and elecampane composition and use of ginger and elecampane composition for preparing toxicity-reducing efficacy-improving medicine in cancer radiotherapy and chemotherapy |
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| CN103393846A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Natural medicine extract and preparation method thereof |
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Application publication date: 20130710 |