CN103191207A - Perpetration for treating chicken intestinal tract escherichia coli diarrhea and preparation method thereof - Google Patents
Perpetration for treating chicken intestinal tract escherichia coli diarrhea and preparation method thereof Download PDFInfo
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- CN103191207A CN103191207A CN2013101083416A CN201310108341A CN103191207A CN 103191207 A CN103191207 A CN 103191207A CN 2013101083416 A CN2013101083416 A CN 2013101083416A CN 201310108341 A CN201310108341 A CN 201310108341A CN 103191207 A CN103191207 A CN 103191207A
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Abstract
The invention relates to the prevention and the treatment of chicken intestinal tract escherichia coli diarrhea, in particular to a synbiotics Chinese herbal composition for preventing and treating the chicken intestinal tract escherichia coli diarrhea, and discloses a traditional Chinese medicine composition for treating the chicken intestinal tract escherichia coli diarrhea. The traditional Chinese medicine composition consists of dianthus superbus, the root of Chinese pulsatilla, liquorice and dandelion; the synbiotics composition of the traditional Chinese medicine composition is proportioned by the traditional Chinese medicine composition and the synbiotics according to the weight ratio of (55-90):(5-10); within two weeks after the chickens go out of the shells, the chicken are fed by the preparation and the feed, so that the preparation can play a part in prevention; the chicken intestinal tract escherichia coli diarrhea can be cured by increasing the dose after the disease comes on; after the traditional Chinese medicines are jointly used, the composition is prominent in the treatment effect for the chicken intestinal tract escherichia coli diarrhea, so that the appetite can be improved; and after the synbiotics preparation is added, the traditional Chinese medicine composition has synergy function.
Description
Technical field
The present invention relates to the control of chickling intestinal coliform diarrhea, especially a kind of symphysis unit Chinese medicine compound of preventing and treating chickling intestinal coliform diarrhea disease.
Background technology
Carnis Gallus domesticus is the leading indicator that improves the vegetable basket project of national life level, also is the key factor that influences price fluctuation.China's Carnis Gallus domesticus imbalance of supply and demand in 2010 causes the price rise of meat valency, causes that country is to the concern of aquaculture.
The poultry husbandry development key once had the saying of " supporting good chickling most receives ", chickling stalwartness, broiler rate of animals delivered to the slaughter-house height chickling.Chickling is born and latter two week is called the chickling phase, at this moment the easiest generation coliform diarrhea.Suffer from the chickling slight of stature of coliform diarrhea, be unwilling to take food, have loose bowels at first, be infectious, intestinal mucosa has catarrhal inflammation, the mesenteric lymph node mild swelling, the sick chicken intraperitoneal of minority has a small amount of glue peptone sample material, the bladder mucosa hyporrhea, and untimely treatment chickling can be dead.Chickling intestinal coliform diarrhea disease " is relied mainly on prevention, is prevented overweighting controlling ", but still has prevention and medicine preferably at present.
Summary of the invention
One of purpose of the present invention is to provide with a kind of Chinese medicine composition, and it has the effect of control chickling intestinal coliform diarrhea disease.
For achieving the above object, technical scheme of the present invention is:
The Chinese medicine composition that is used for the treatment of chickling intestinal coliform diarrhea is made up of the dry medical material of following weight portion: Herba Dianthi 35-50 part, Radix Pulsatillae 3-8 part, Radix Glycyrrhizae 5-15 part, Herba Taraxaci 8-25 part.
Further, described Chinese medicine composition is made up of the dry medical material of following weight portion: 45 parts of Herba Dianthis, 6 parts of the Radix Pulsatillaes, 13 parts in Radix Glycyrrhizae, 22 parts of Herba Taraxacis.
Two of purpose of the present invention is to provide with a kind of symphysis unit's compositions and preparation and the feedstuff that contains said preparation, and they add symphysis unit element on the basis of described Chinese medicine composition, collaborative control chickling intestinal coliform diarrhea disease.
For achieving the above object, technical scheme of the present invention is:
Formed according to the weight ratio proportioning of 55-90: 5-10 by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed by bifidobacterium preparations and the oligosaccharide weight ratio proportioning according to 3-5: 5-10, containing the bifidobacteria viable bacteria number in the described every gram symphysis unit is hundred million of 200-450, and described oligosaccharide is any one or more in fruit oligose, oligomeric xylose, dextrinosan and the soybean oligo saccharide.
Further, described symphysis unit compositions is formed according to 80: 7 weight ratio proportioning by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed by bifidobacterium preparations and the oligosaccharide weight ratio proportioning according to 5: 8, and containing the bifidobacteria viable bacteria number in the described every gram symphysis unit is 40,000,000,000, and described oligosaccharide is soybean oligo saccharide
The preparation of the first compositions of described symphysis.
The feedstuff that contains the preparation of the first compositions of described symphysis.
Further, contain the preparation that mass fraction is the first compositions of described symphysis of 8-15% in the described feedstuff.
Three of purpose of the present invention is to provide the preparation method of the preparation of symphysis unit compositions, and this method is simple to operate, and process stabilizing is applicable to suitability for industrialized production.
For achieving the above object, technical scheme of the present invention is:
The preparation method of described preparation specifically may further comprise the steps: with the 4-6% inoculation that bacillus bifidus is pressed culture volume, be 6.0-8.0 at pH value, 37 ℃ of constant temperature leave standstill to be cultivated 24-72 hour, got bifidobacterium preparations; The bacillus bifidus culture medium is calculated composed of the following components by weight: peptone 0.1-1.0 part, Carnis Bovis seu Bubali cream 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilization; The bifidobacterium preparations of formula ratio and the oligosaccharide of formula ratio are fully mixed, get described symphysis unit; The decocting for Chinese herbal medicine liquid that described symphysis is first and described Chinese medicine composition makes of formula ratio is mixed, get the preparation of symphysis unit compositions, the concentration of bacillus bifidus is more than or equal to 1 * 10 in the preparation of the first compositions of described symphysis
7Individual/mL.
Further, in the 6 described preparation methoies, described bifidobacterium preparations specifically may further comprise the steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification inoculation and go into to be equipped with the bacillus bifidus culture medium in vitro, 37 ℃ of constant temperature leave standstill to be cultivated 24 hours, obtained the first order seed bacterium, stored refrigerated;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of bacillus bifidus culture volume, be inoculated in the bacillus bifidus culture medium of sterilizing and cooling off, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium; Stored refrigerated; The bacillus bifidus culture medium is poured in the microorganism fermentation tank into sterilization and cooling;
The C fermentation culture
With the ratio inoculation of step B gained secondary seed bacterium in the 6-8% of the bacillus bifidus culture volume of sterilizing and cooling off, stir, 37 ℃ of constant temperature leave standstill cultivates 48-72h, gets culture fluid;
The D centrifuge dehydration
Supernatant in the centrifugal 20min of 3000r/min, is removed in step C gained culture fluid cooling back, get bacterium mud, interpolation is equivalent to bacterium mud weight 1-2 filler mix homogeneously doubly, places under the 40-50 ℃ of condition and dries, and gets bifidobacterium preparations.
Further, in the described preparation method: the bacillus bifidus culture medium prescription: 0.6 part of peptone, 0.3 part of Carnis Bovis seu Bubali cream, 0.15 part of cysteine, 0.5 part in sodium chloride, 1.2 parts of glucoses, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
Beneficial effect of the present invention is: the Chinese medicine composition that is used for the treatment of chickling intestinal coliform diarrhea of the present invention, made by Herba Dianthi, the Radix Pulsatillae, Radix Glycyrrhizae, Herba Taraxaci, and adopt water-boiling method acquisition medicinal liquid to add symphysis unit again and form.Go out shell in latter two week chickling, with this medicine and feedstuff mixing feeding chickling, can play preventive effect; Dose is strengthened in the morbidity back can treat recovery from illness.Wherein, Herba Dianthi is sliding sharp, can fall YIN-fire, except five types of stranguria, sharp blood vessels, and the medicine of a cold nature of holding concurrently.Radix Pulsatillae bitter in the mouth, cold in nature; Return stomach, large intestine channel, have heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, the effect of removing dampness and killing parasites.Radix Glycyrrhizae has heat-clearing and toxic substances removing, effects such as gastral cavity abdomen.Herba Taraxaci has heat-clearing and toxic substances removing, the effect of dispersing swelling and dissipating binds.Several Chinese medicines are united utilization, treatment chickling intestinal coliform diarrhea is had the significant curative effect curative effect, and can improve appetite.After adding symphysis unit preparation, has synergistic function.
The specific embodiment
Embodiment 1 is used for the treatment of the preparation of the preparation of chickling intestinal coliform diarrhea
One, taking by weighing oligosaccharide is that each 10Kg of fruit oligose, oligomeric xylose, dextrinosan and soybean oligo saccharide is standby;
Two, the preparation of bifidobacterium preparations:
Strain is the bacillus bifidus (Bifidobacterium) that lyophilizing is preserved; The bacillus bifidus culture medium is calculated composed of the following components by weight: 0.6 part of peptone, 0.3 part of Carnis Bovis seu Bubali cream, 0.15 part of cysteine, 0.5 part in sodium chloride, 1.2 parts of glucoses, 0.2 part of hepar siccatum, 95 parts of aqueous mediums, pH6.8,115 ℃ of high pressure steam sterilization 30min;
1. enlarge kind of a daughter bacteria, get strain on the inclined-plane (as an inoculating loop) inoculation and go into to be equipped with the bacillus bifidus culture medium in vitro, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the first order seed bacterium, takes out refrigerator and preserves, stand-by;
2. the preparation of secondary seed bacterium: with 5% the ratio of first order seed bacterium in the volume of bacillus bifidus culture medium, be inoculated in the bacillus bifidus bacterium culture medium, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium;
3. produce the preparation of the bacillus bifidus culture medium of usefulness: take by weighing each composition in the composition ratio of bacillus bifidus culture medium and be dissolved in water, pour in the microorganism fermentation tank, in 115 ℃ of steam sterilizations 30 minutes;
4. inoculation: the bacillus bifidus culture medium of usefulness to be produced is chilled to about 37 ℃, adds the secondary seed bacterium under flame control, inoculates by the ratio of the 6-8% of bacillus bifidus culture volume, stirs;
5. cultivate: close all breather valve, 37 ℃ of constant temperature culture 60h;
6. centrifuge dehydration: in biological fermentation tank, take out culture fluid, refrigerator is temporary, treat culture fluid cooling back in 3000 rev/mins centrifugal 20 minutes, remove supernatant, get bacterium mud and add 2 times of bran powder mix homogeneously, the disk of packing into places oven dry in 45 ℃ of air dry ovens, carries out bacillus bifidus by the GB4789-2012 method and identifies, and calculate total viable count, pack, get bifidobacterium preparations, keep in Dark Place standby at shady and cool dry place.
Three are used for the treatment of the preparation of the preparation of chickling intestinal coliform diarrhea
1 is used for the treatment of the Chinese medicine composition composition formula of chickling intestinal coliform diarrhea
The Chinese medicine composition that is used for the treatment of chickling intestinal coliform diarrhea, be called for short Chinese medicine composition 1: the dry medical material by following weight portion is formed: 35 parts of Herba Dianthis, 3 parts of the Radix Pulsatillaes, 15 parts in Radix Glycyrrhizae, 25 parts of Herba Taraxacis.
The Chinese medicine composition that is used for the treatment of chickling intestinal coliform diarrhea, be called for short Chinese medicine composition 2: the dry medical material by following weight portion is formed: 50 parts of Herba Dianthis, 8 parts of the Radix Pulsatillaes, 5 parts in Radix Glycyrrhizae, 8 parts of Herba Taraxacis.
The Chinese medicine composition that is used for the treatment of chickling intestinal coliform diarrhea, be called for short Chinese medicine composition 3: the dry medical material by following weight portion is formed: 45 parts of Herba Dianthis, 6 parts of the Radix Pulsatillaes, 13 parts in Radix Glycyrrhizae, 22 parts of Herba Taraxacis.
2 contain the prescription of the symphysis unit compositions of described Chinese medicine composition
The symphysis unit compositions that contains above-mentioned Chinese medicine composition 1 is called for short symphysis unit compositions 1: formed according to 55: 10 weight ratio proportioning by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed by bacillus bifidus system and the oligosaccharide weight ratio proportioning according to 3: 10, and containing the bifidobacteria viable bacteria number in the described every gram symphysis unit is 20,000,000,000, and described oligosaccharide is oligomeric xylose.
The symphysis unit compositions that contains above-mentioned Chinese medicine composition 2 is called for short symphysis unit compositions 2: formed according to 90: 5 weight ratio proportioning by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed by bacillus bifidus system and the oligosaccharide weight ratio proportioning according to 1: 1, and containing the bifidobacteria viable bacteria number in the described every gram symphysis unit is 45,000,000,000, and described oligosaccharide is fruit oligose.
The symphysis unit compositions that contains above-mentioned Chinese medicine composition 3 is called for short symphysis unit compositions 3: formed according to 80: 7 weight ratio proportioning by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed by bifidobacterium preparations and the oligosaccharide weight ratio proportioning according to 5: 8, and containing the bifidobacteria viable bacteria number in the described every gram symphysis unit is 40,000,000,000, and described oligosaccharide is soybean oligo saccharide.
3 are used for the treatment of the preparation of the preparation of chickling intestinal coliform diarrhea
The soybean oligo saccharide of formula ratio in the described symphysis unit compositions 3 of the bifidobacterium preparations of embodiment 1 preparation and embodiment 3 is fully mixed, get described symphysis unit; The described symphysis unit of described symphysis unit compositions 3 formula ratios of embodiment 3 and the decocting for Chinese herbal medicine liquid that described Chinese medicine composition makes are mixed, got the preparation of symphysis unit compositions, bacillus bifidus equals 1 * 10 in the preparation of the first compositions of described symphysis
7Individual/mL.
Each component in the said herbal medicine additive, see " national Chinese herbal medicine compilation " (Xie Zongwan chief editor for details, the People's Health Publisher, 1996-1052) and " Chinese medicine voluminous dictionary " (Nanjing University of Traditional Chinese Medicine writes, Shanghai science tech publishing house publication time: the 2006-3-1 second edition.
Embodiment 2 is used for the treatment of the effect of the preparation of chickling intestinal coliform diarrhea
The effect of one treatment chickling intestinal coliform diarrhea
In normal feedstuff, add the preparation of the symphysis unit compositions of embodiment 1 preparation, must contain the feedstuff of the preparation of the first compositions of described symphysis.The preparation that contains mass fraction in the described feedstuff and be the described symphysis unit compositions of 8-15% all can.
1) 160 chickling (every chickling is independently raised) are divided into experimental group and matched group.80 of experimental grouies mix hello chickling 7 days with preparation 280 grams of above-mentioned symphysis unit compositions with 2000 gram normal feedstuff.80 of matched groups were fed chickling 7 days with normal feedstuff.Wherein, the intestinal coliform diarrhea does not take place in the experimental group chickling; The incidence rate of matched group coliform diarrhea is for being 20%.
20 chickling of 2 pairs of matched groups morbidity contain the standard feeding 5 days of feedstuff of the preparation of described symphysis unit compositions by every every meal 110g, 14 chickling coliform diarrheas are cured, and after this do not recur.
The diagnosis of chickling intestinal coliform diarrhea is with reference to village a kind of jade " diagnosis of chickling Escherichia coli disease " literary composition.
Two feed effects relatively
160 chickling of the same age are divided into experimental group and matched group at random.80 of experimental grouies mix hello chickling 5 days with preparation 160 grams of above-mentioned symphysis unit compositions with 2000 gram normal feedstuff.80 of matched groups were fed chickling 5 days with normal feedstuff.
After feeding was finished in five days, record the daily inleting appetite that feeding is finished the 2nd day, the 3rd day and the 5th day respectively.Daily inleting appetite be calculated as matched group or experimental group feed total amount divided by chickling quantity, i.e. the per day food-intake of every chickling.
The per day food-intake of table 1 chickling
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (10)
1. be used for the treatment of the Chinese medicine composition of chickling intestinal coliform diarrhea, it is characterized in that: the dry medical material by following weight portion is formed: Herba Dianthi 35-50 part, Radix Pulsatillae 3-8 part, Radix Glycyrrhizae 5-15 part, Herba Taraxaci 8-25 part.
2. Chinese medicine composition according to claim 1, it is characterized in that: the dry medical material by following weight portion is formed: 45 parts of Herba Dianthis, 6 parts of the Radix Pulsatillaes, 13 parts in Radix Glycyrrhizae, 22 parts of Herba Taraxacis.
3. contain the symphysis unit compositions of claim 1 or 2 described Chinese medicine compositions, it is characterized in that: formed according to the weight ratio proportioning of 55-90: 5-10 by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed by bifidobacterium preparations and the oligosaccharide weight ratio proportioning according to 3-5: 5-10, containing the bifidobacteria viable bacteria number in the described every gram symphysis unit is hundred million of 200-450, and described oligosaccharide is any one or more in fruit oligose, oligomeric xylose, dextrinosan and the soybean oligo saccharide.
4. symphysis according to claim 3 unit compositions is characterized in that: formed according to 80: 7 weight ratio proportioning by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed by bifidobacterium preparations and the oligosaccharide weight ratio proportioning according to 5: 8, and containing the bifidobacteria viable bacteria number in the described every gram symphysis unit is 40,000,000,000, and described oligosaccharide is soybean oligo saccharide.
5. the preparation of the described symphysis of claim 3 unit compositions.
6. the preparation method of the described preparation of claim 5 is characterized in that, specifically may further comprise the steps: with the 4-6% inoculation that bacillus bifidus is pressed culture volume, be 6.0-8.0 at pH value, 37 ℃ of constant temperature leave standstill to be cultivated 24-72 hour, got bifidobacterium preparations; The bacillus bifidus culture medium is calculated composed of the following components by weight: peptone 0.1-1.0 part, Carnis Bovis seu Bubali cream 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilization; The bifidobacterium preparations of formula ratio and the oligosaccharide of formula ratio are fully mixed, get described symphysis unit; The decocting for Chinese herbal medicine liquid that described symphysis is first and described Chinese medicine composition makes of formula ratio is mixed, get the preparation of symphysis unit compositions, the concentration of bacillus bifidus is more than or equal to 1 * 10 in the preparation of the first compositions of described symphysis
7Individual/mL.
7. preparation method according to claim 6 is characterized in that, described bifidobacterium preparations specifically may further comprise the steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification inoculation and go into to be equipped with the bacillus bifidus culture medium in vitro, 37 ℃ of constant temperature leave standstill to be cultivated 24 hours, obtained the first order seed bacterium, stored refrigerated;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of bacillus bifidus culture volume, be inoculated in the bacillus bifidus culture medium of sterilizing and cooling off, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium; Stored refrigerated; The bacillus bifidus culture medium is poured in the microorganism fermentation tank into sterilization and cooling;
The C fermentation culture
With the ratio inoculation of step B gained secondary seed bacterium in the 6-8% of the bacillus bifidus culture volume of sterilizing and cooling off, stir, 37 ℃ of constant temperature leave standstill cultivates 48-72h, gets culture fluid;
The D centrifuge dehydration
Supernatant in the centrifugal 20min of 3000r/min, is removed in step C gained culture fluid cooling back, get bacterium mud, interpolation is equivalent to bacterium mud weight 1-2 filler mix homogeneously doubly, places under the 40-50 ℃ of condition and dries, and gets bifidobacterium preparations.
8. preparation method according to claim 7 is characterized in that: the bacillus bifidus culture medium prescription: 0.6 part of peptone, 0.3 part of Carnis Bovis seu Bubali cream, 0.15 part of cysteine, 0.5 part in sodium chloride, 1.2 parts of glucoses, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
9. the feedstuff that contains the preparation of the described symphysis of claim 5 unit compositions.
10. feedstuff according to claim 9 is characterized in that, containing mass fraction in the described feedstuff is the preparation of the first compositions of described symphysis of 8-15%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105535387A (en) * | 2014-11-03 | 2016-05-04 | 孙文彬 | Ferment for improving livestock and poultry intestinal diarrhea, and preparation method thereof |
| CN106074812A (en) * | 2016-08-29 | 2016-11-09 | 兴文县鸿程农业生态科技开发有限责任公司 | A kind of Chinese medicine compound for preventing and treating bacterial disease of fowl and application |
| CN108783076A (en) * | 2018-06-28 | 2018-11-13 | 浦北县清华生态养鸡专业合作社 | Prevent the preparation method of chicken colibacillosis feed |
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| CN102764313A (en) * | 2012-08-14 | 2012-11-07 | 天津生机集团股份有限公司 | Drug for preventing swine winter dysentery and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105535387A (en) * | 2014-11-03 | 2016-05-04 | 孙文彬 | Ferment for improving livestock and poultry intestinal diarrhea, and preparation method thereof |
| CN105535387B (en) * | 2014-11-03 | 2018-05-08 | 孙文彬 | Animal and bird intestines diarrhea ferment and preparation method thereof can be improved |
| CN106074812A (en) * | 2016-08-29 | 2016-11-09 | 兴文县鸿程农业生态科技开发有限责任公司 | A kind of Chinese medicine compound for preventing and treating bacterial disease of fowl and application |
| CN106074812B (en) * | 2016-08-29 | 2020-01-21 | 兴文县鸿程农业生态科技开发有限责任公司 | Traditional Chinese medicine compound for preventing and treating poultry bacterial diseases and application |
| CN108783076A (en) * | 2018-06-28 | 2018-11-13 | 浦北县清华生态养鸡专业合作社 | Prevent the preparation method of chicken colibacillosis feed |
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