CN103168965A - Anti-stress synbiotics traditional Chinese medicine composition applicable to chicken and preparation method of anti-stress synbiotics traditional Chinese medicine composition - Google Patents
Anti-stress synbiotics traditional Chinese medicine composition applicable to chicken and preparation method of anti-stress synbiotics traditional Chinese medicine composition Download PDFInfo
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Abstract
The invention relates to the field of livestock breeding and particularly relates to an anti-stress synbiotics traditional Chinese medicine composition for chicken. The anti-stress synbiotics traditional Chinese medicine composition consists of a micro-ecological synbiotics feed additive and a Chinese herbal medicine additive, wherein the micro-ecological synbiotics feed additive consists of the following components in parts by weight: 10-60 parts of micro-ecological probiotics and 40-90 parts of oligosaccharide, 20-45 billion living bifidobacteria are contained in per gram of micro-ecological synbiotics feed additive, the micro-ecological probiotics are a bifidobacteria preparation, the oligosaccharide is any one or more of fructooligosaccharide, xylooligosaccharide, isomaltooligosacharide and soybean oligosaccharides, the micro-ecological synbiotics feed additive accounts for 1-2% of the total weight of the anti-stress synbiotics traditional Chinese medicine composition for chicken, and the Chinese herbal medicine additive consists of the following components in parts by weight: 3-5 parts of schisandra, 10-15 parts of keels, 15-18 parts of zizyphus jujube kernel, 10-20 parts of nymphaea tetragona and 25-30 parts of clover. The anti-stress synbiotics traditional Chinese medicine composition can be used for improving the immunity and anti-stress capability of the chicken, and reducing the incidence of diseases.
Description
Technical field
The present invention relates to the livestock rearing field, particularly micro-ecological feed and preparation method thereof.
Background technology
What is called stress (stress) refer to a kind of nonspecific reaction that animal shows the poor environment factor.These factors comprise: the flip-flop of living environment as transportation, turn the crowded and trample in group and when resettlement etc.; The flip-flop of the flip-flop of environmental condition such as the variation of weather, light application time and intensity; The flip-flop of the change of nutritional status such as the kind of feed, quality, quantity, feed formula is unreasonable, the long-term lacking of some nutritional labeling or excessive; Deterioration such as the cultivation density of living environment is too high, harmful gas concentration is excessive, the dysdipsia of searching for food, water quality inferiority, ventilation is bad, dust is many, pathogenic microorganism is many, sanitary condition is poor etc.; The flip-flop of feeding and management program is as grabbing chicken, docking, injection, controlled feeding, suddenly clear excrement etc.; Disease is undoubtedly stress factor, and dispensing also easily cause stress.The phosphamidon class medical instrument of high dose has peculiar smell, excitant and toxicity, and mordant disinfectant is used for that the drinking-water, spraying, sterilization of feeding process etc. easily causes stress; Stressor because of some vaccine and immunologic process thereof can cause stress.The unexpected variation of the unexpected movement of object, unexpected noise, work clothes color (particularly red) etc. can cause all that livestock and poultry are excited and produce stress.In fact, all can cause that the livestock and poultry body does not feel like oneself and causes the factor of body damage all to can be described as stressor.
Chick is that a class is to the highstrung animal of environmental change.Chick is under stress situation, often shows the symptoms such as apocleisis, dysphoria, growth are slowed down, death rate rising.Generally take the pattern of high density culture for pursuing cultured output because the uncontrollability of Changes in weather adds China raiser, therefore, in breeding process, in various degree stress phenomenon inevitable occurs in chick.Due to stress, annual give direct economic loss that China's chick breeding industry brings over 2,000,000,000 yuan.Therefore, a kind of effect of exploitation is remarkable, effect is stable, chick Antistress agent tool easy to use is of great significance.
At present, be used for livestock and poultry anti-stress medicine or additive, mostly be chemicals, the long-term use, can cause not only that the livestock and poultry body develops immunity to drugs, stress reaction, the bad reaction such as poisoning, and its harmful components residue in the livestock products such as meat, egg, milk, can directly be detrimental to health.Modern medicine study shows, in animal derived food, antibiotic residue may cause the human DNA structure to be undergone mutation, thereby causes the serious consequences such as " disabling ", " teratogenesis ", " carcinogenic ".
Natural Chinese medicinal herb and probiotics preparation have no side effect with its uniqueness, and without hazard residue, the advantage such as non-stress does not produce drug resistance, and is free from environmental pollution becomes the benefit-additives of producing green animal food and emphasis and the focus of herding worker research.External approved uses as the feed medicated premix.Along with further developing of China's animal husbandry and animal products foreign trade.Must pay attention to the reasonable use of medicated premix.
Summary of the invention
The object of the present invention is to provide the composition that forms with a kind of little ecological symphysis unit's feed addictive and Chinese herbal feed, the number of viable that described additive contains is high, the stress reaction for the treatment of chick with the Feed Energy of this additive preparation.
For achieving the above object, technical scheme of the present invention is:
Chick anti-stress symphysis unit's Chinese medicine composition, formed by little ecological symphysis unit's feed addictive and Chinese herb feed additive, the first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part, oligosaccharides 40-90 part, containing the number of active bifid bacteria in the every gram of described little ecological symphysis unit's feed addictive is hundred million of 200-450, described little ecological probio is bifidobacterium preparations, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides; Described little ecological symphysis unit feed addictive account for described chick anti-stress symphysis the 1-2% of unit's Chinese medicine composition gross weight, described Chinese herb feed additive is composed of the following components by weight: fruit of Chinese magnoliavine 3-5 part, keel 10-15 part, spina date seed 15-18 part; Water lily 10-20 part; Clover 25-30 part.
Further, the little ecological symphysis of described Chinese herbal medicine unit feed addictive, in described little ecological symphysis unit feed addictive, to contain the number of active bifid bacteria be 40,000,000,000 to every gram.
Further, described chick anti-stress symphysis unit's Chinese medicine composition, described little ecological symphysis unit feed addictive account for described chick anti-stress symphysis unit's Chinese medicine composition gross weight 1.5%, described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 12 parts of keel, 16 parts of spina date seeds; 18 parts of water lilys; 27 parts of clover.
Contain described chick anti-stress symphysis the feed of unit's Chinese medicine composition.
Further, contain in described feed mass fraction be the described chick of 1.5-2% anti-stress symphysis unit's Chinese medicine composition.
Another object of the present invention is to provide above-mentioned chick anti-stress symphysis the preparation method of preparation of unit's Chinese medicine composition, the method is simple to operate, production cost is low, is applicable to large-scale production.
Described chick anti-stress symphysis the preparation method of preparation of unit's Chinese medicine composition, the 4-6% that Bifidobacterium press culture volume inoculates, and is 6.0-8.0 in the pH value, 37 ℃ of standing cultivations of constant temperature 24-72 hour get bifidobacterium preparations; Medium of Bifidobacterium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilization; The bifidobacterium preparations of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Described little ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, get chick anti-stress symphysis the preparation of unit's Chinese medicine composition.
Further, in described preparation method, described bifidobacterium preparations specifically comprises the following steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification and inoculate into Medium of Bifidobacterium being housed in vitro, 37 ℃ of standing cultivations of constant temperature 24 hours obtain the first order seed bacterium, and are stored refrigerated stand-by;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of Medium of Bifidobacterium volume, be inoculated into sterilize and cooling Medium of Bifidobacterium in, the 37 ℃ of standing cultivation of constant temperature 24h obtain the secondary seed bacterium; Take out Refrigerator store, stand-by; Medium of Bifidobacterium is poured in microorganism fermentation tank, and sterilization is also cooling;
The C fermented and cultured
Step B gained secondary seed bacterium in the also ratio inoculation of the 6-8% of cooling Medium of Bifidobacterium volume of sterilizing, is stirred, and the 37 ℃ of standing cultivation of constant temperature 48-72h get nutrient solution;
The D centrifugal dehydration
In the centrifugal 20min of 3000r/min, remove supernatant after step C gained nutrient solution is cooling, get bacterium mud, add the filler that is equivalent to bacterium mud weight 1-2 times and mix, be placed under 40-50 ℃ of condition and dry, get bifidobacterium preparations.
Further, in described preparation method, Medium of Bifidobacterium formula: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part, sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
Beneficial effect of the present invention is: 1) compare with alone ecological symphysis unit feed addictive and have the following advantages: can improve chicken immunity and anti-stress ability, reduce the incidence of disease, the few or not medication of medication is conducive to health of human body and environmental protection; Be fit to modernization industry and produce, constant product quality; 2) Chinese herbal medicine in this formula, the power of fruit of Chinese magnoliavine energy strengthening by means of tonics has the tonifying liver effect; Keel have the tranquillizing the mind by relieving convulsion, arrest sweating controlling nocturnal emission with astringent drugs, the puckery intestines that stop blooding, the effect of regenerating tissue to heal wond; Spina date seed has analgesia, anticonvulsion, cooling effect; Water lily has wet brain calms the nerves, and the effect of hot nourishing the liver falls in the heat-clearing bushing; Clover has heat-clearing, the effect of cool blood; Use in conjunction is obvious to the chick anti-stress ability.
The specific embodiment
Embodiment 1 chick anti-stress symphysis the preparation of unit's Chinese medicinal composition preparation
One, taking oligosaccharides is that each 10Kg of FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides is standby;
Two, the preparation of bifidobacterium preparations:
Bacterial classification is the Bifidobacterium (Bifidobacterium) that freeze-drying is preserved; Medium of Bifidobacterium calculates composed of the following components by weight: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part, sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums, pH6.8,115 ℃ of high pressure steam sterilization 30min;
1. enlarge kind of a daughter bacteria, get bacterial classification on the inclined-plane (as an oese) inoculation and enter to be equipped with Medium of Bifidobacterium in vitro, the 37 ℃ of standing cultivation of constant temperature 24h obtain the first order seed bacterium, take out Refrigerator store, and are stand-by;
2. the preparation of secondary seed bacterium: with 5% the ratio of first order seed bacterium in the volume of Medium of Bifidobacterium, be inoculated in the Bifidobacterium bacterium culture medium, the 37 ℃ of standing cultivation of constant temperature 24h obtain the secondary seed bacterium;
3. produce the preparation of the Medium of Bifidobacterium of use: take each composition in the composition ratio of Medium of Bifidobacterium and be dissolved in water, pour in microorganism fermentation tank, in 115 ℃ of steam sterilizations 30 minutes;
4. inoculation: the Medium of Bifidobacterium of use to be produced is chilled to 37 ℃ of left and right, adds the secondary seed bacterium under flame is controlled, and inoculates by the ratio of the 6-8% of Medium of Bifidobacterium volume, stirs;
5. cultivate: close all breather valves, 37 ℃ of constant temperature culture 60h;
6. centrifugal dehydration: take out nutrient solution in biological fermentation tank, refrigerator is temporary, after nutrient solution is cooling in 3000 rev/mins centrifugal 20 minutes, remove supernatant, getting bacterium mud adds 2 times of chaff powder to mix, the disk of packing into is placed in oven dry in 45 ℃ of air dry ovens, carries out the Bifidobacterium evaluation by the GB4789-2012 method, and calculate total viable count, pack, get bifidobacterium preparations, keep in Dark Place standby at shady and cool dry place.
Three chick anti-stress symphysis the preparation of preparation of unit's Chinese medicine composition
1) little ecological symphysis unit's feed addictive prescription one (being called for short additive 1): calculate by weight composed of the following components: 50 parts of bifidobacterium preparations, 90 parts of FOSs, wherein, in the first feed addictive of the ecological symphysis of every gram, viable count is 30,000,000,000.
Chick anti-stress symphysis one (being called for short composition 1) of unit's Chinese traditional medicine composition composition formula: additive 1 account for described chick anti-stress symphysis unit's Chinese medicine composition 1 gross weight 1%, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), described Chinese herb feed additive is composed of the following components by weight: 3 parts, the fruit of Chinese magnoliavine, 15 parts of keel, 15 parts of spina date seeds; 20 parts of water lilys; 30 parts of clover.
2) little ecological symphysis unit's feed addictive prescription two (being called for short additive 2): calculate by weight composed of the following components: 60 parts of bifidobacterium preparations, 40 parts of soyabean oligosaccharides, wherein, in the first feed addictive of the ecological symphysis of every gram, viable count is 45,000,000,000.
Chick anti-stress symphysis one (being called for short composition 2) of unit's Chinese traditional medicine composition composition formula: additive 1 account for described chick anti-stress symphysis unit's Chinese medicine composition 2 gross weights 2%, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 10 parts of keel, 18 parts of spina date seeds; 10 parts of water lilys; 25 parts of clover.
3) little ecological symphysis unit's feed addictive prescription three (being called for short additive 3): calculate by weight composed of the following components: 20 parts of bifidobacterium preparations, 80 parts of xylo-oligosaccharides, wherein, in the first feed addictive of the ecological symphysis of every gram, viable count is 40,000,000,000.
Chick anti-stress symphysis one (being called for short composition 3) of unit's Chinese traditional medicine composition composition formula: additive 1 account for described chick anti-stress symphysis unit's Chinese medicine composition 3 gross weights 1.5%, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 12 parts of keel, 16 parts of spina date seeds; 18 parts of water lilys; 27 parts of clover.The mouthfeel of this formula is best.
Each component in the said herbal medicine additive, see " national Chinese herbal medicine compilation " (Xie Zongwan chief editor for details, the People's Health Publisher, 1996-1052) and " Chinese medicine voluminous dictionary " (Nanjing University of Traditional Chinese Medicine writes, Shanghai science tech publishing house publication time: the 2006-3-1 second edition.
Each component of formula ratio is mixed in proportion, and the granulation agent is so that quality control, preservation, transportation and use.
Embodiment 2 chick anti-stress symphysis the application of preparation of unit's Chinese medicine composition
One, experiment grouping
1 experimental group
Be chosen at 300 chick that occur the transport stress reaction in transportation, freely ingest and contain the feed of the described composition 2 of embodiment 1.The chick that adds in described feed anti-stress symphysis the mass percent of preparation of unit's Chinese medicine composition be 2%.
2 control groups
Be chosen at 50 chick that occur the transport stress reaction in transportation, edible normal diet.
Two, index detects
Blood is got in blood sample acquisition method oxter, and blood sample was taked after respectively at front 1 day of administration, administration in 3 days.Every each blood sampling 0.5ml, separation of serum is put-20 ℃ of refrigerators, does the Biochemical Indices In Serum check, completes in 4d.
Biochemical investigation method Physiological-biochemical Parameters send laboratory, the People's Hospital, Rongchang County to detect.
The serum glucose of experimental group and control group chick, serum cortisol, urea nitrogen assay see Table 1.By as seen from Table 1, test group serum glucose, serum cortisol, urea nitrogen average are tending towards descending, and through the t check, with control group, utmost point significant difference (P＜0.01) are arranged.
The variation of table 1 serum glucose, T4, urea nitrogen
Experimental group is continuously in edible one week of feed of containing the described composition 2 of embodiment 1.After control group and experimental group were under equal conditions raised January respectively, the death rate of control group was 23%, and the experimental group death rate is 4%.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment, the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not breaking away from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (8)
- Chick anti-stress symphysis unit's Chinese medicine composition, it is characterized in that, formed by little ecological symphysis unit's feed addictive and Chinese herb feed additive, the first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part, oligosaccharides 40-90 part, containing the number of active bifid bacteria in the every gram of described little ecological symphysis unit's feed addictive is hundred million of 200-450, described little ecological probio is bifidobacterium preparations, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides; Described little ecological symphysis unit feed addictive account for described chick anti-stress symphysis the 1-2% of unit's Chinese medicine composition gross weight, described Chinese herb feed additive is composed of the following components by weight: fruit of Chinese magnoliavine 3-5 part, keel 10-15 part, spina date seed 15-18 part; Water lily 10-20 part; Clover 25-30 part.
- Chick according to claim 1 anti-stress symphysis unit's Chinese medicine composition, it is characterized in that, in described little ecological symphysis unit feed addictive, to contain the number of active bifid bacteria be 40,000,000,000 to every gram.
- Chick according to claim 1 anti-stress symphysis unit's Chinese medicine composition, it is characterized in that: described little ecological symphysis unit feed addictive account for described chick anti-stress symphysis unit's Chinese medicine composition gross weight 1.5%, described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 12 parts of keel, 16 parts of spina date seeds; 18 parts of water lilys; 27 parts of clover.
- The described chick of claim 1-3 any one anti-stress symphysis the preparation method of preparation of unit's Chinese medicine composition, it is characterized in that, Bifidobacterium is pressed the 4-6% inoculation of culture volume, is 6.0-8.0 in the pH value, 37 ℃ of standing cultivations of constant temperature 24-72 hour get bifidobacterium preparations; Medium of Bifidobacterium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilization; The bifidobacterium preparations of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Described little ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, get chick anti-stress symphysis the preparation of unit's Chinese medicine composition.
- 5. preparation method according to claim 4, is characterized in that, described bifidobacterium preparations specifically comprises the following steps:A enlarges kind of a daughter bacteriaGet on the inclined-plane bacterial classification and inoculate into Medium of Bifidobacterium being housed in vitro, 37 ℃ of standing cultivations of constant temperature 24 hours obtain the first order seed bacterium, and are stored refrigerated;The preparation of B secondary seed bacteriumWith the ratio of first order seed bacterium in the 4-6% of Medium of Bifidobacterium volume, be inoculated into sterilize and cooling Medium of Bifidobacterium in, the 37 ℃ of standing cultivation of constant temperature 24h obtain the secondary seed bacterium; Stored refrigerated; Medium of Bifidobacterium is poured in microorganism fermentation tank, and sterilization is also cooling;The C fermented and culturedStep B gained secondary seed bacterium in the also ratio inoculation of the 6-8% of cooling Medium of Bifidobacterium volume of sterilizing, is stirred, and the 37 ℃ of standing cultivation of constant temperature 48-72h get nutrient solution;The D centrifugal dehydrationIn the centrifugal 20min of 3000r/min, remove supernatant after step C gained nutrient solution is cooling, get bacterium mud, add the filler that is equivalent to bacterium mud weight 1-2 times and mix, be placed under 40-50 ℃ of condition and dry, get bifidobacterium preparations.
- 6. preparation method according to claim 5, is characterized in that: the Medium of Bifidobacterium formula: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part, sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
- 7. contain chick claimed in claim 1 anti-stress symphysis the feed of unit's Chinese medicine composition.
- 8. feed according to claim 7, is characterized in that, contain mass fraction in described feed and be the described chick of 1.5-2% anti-stress symphysis unit's Chinese medicine composition.
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| CN105477225B (en) * | 2015-12-18 | 2021-08-13 | 河北农业大学 | Traditional Chinese veterinary medicine for enhancing anti-stress capability of chicks, preparation method and feeding method |
| CN106666195A (en) * | 2016-12-20 | 2017-05-17 | 钦州市康绿宝农业有限公司 | Chick feed additive and preparation method thereof |
| CN107173622A (en) * | 2017-06-21 | 2017-09-19 | 安徽省金安禽业有限公司 | A kind of nutritious resisting stress egg feedstuff |
| CN108157664A (en) * | 2018-02-11 | 2018-06-15 | 四川达邦生物科技有限公司 | A kind of feed addictive containing composite vegetables extractive and its preparation process for being used to improve poultry immunity |
| CN108323622A (en) * | 2018-02-11 | 2018-07-27 | 四川达邦生物科技有限公司 | A kind of preparation method of the feed addictive containing composite vegetables extractive |
| CN108740306A (en) * | 2018-05-03 | 2018-11-06 | 武汉环绿时代生态园林工程有限公司 | A kind of feed addictive and its feeding method |
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