CN103168622B - Cultivating method of morchella importuna - Google Patents
Cultivating method of morchella importuna Download PDFInfo
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- CN103168622B CN103168622B CN201310115468.0A CN201310115468A CN103168622B CN 103168622 B CN103168622 B CN 103168622B CN 201310115468 A CN201310115468 A CN 201310115468A CN 103168622 B CN103168622 B CN 103168622B
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Abstract
The invention discloses a cultivating method of morchella importuna. The cultivating method of the morchella importuna comprises a bacterium culturing producing step, a cultivation material producing step, a hydration processing step, a managing and protecting step and the like. A maternal species (HKAS 62868) which can be identified as the morchella importuna (the number of the Genbank is JQ 618576) through a molecular identification can be used for cultivating cultispecieses. After the hydration processing is carried out on the cultispecieses, the cultispecieses are sowed on the face of the right land and then are earthed up. The cultivation materials can be produced and added in the field and the field can be normally managed for 80-120 days, so that sporocarps can be obtained. The cultivating method of the morchella importuna aims at providing an edible mushroom cultivating method which not only is beneficial to the sustainable development of ecology, but also helps to increase farmer income and a novel cultivating bacterial strain is provided for the industrialization development of the morchella importuna at the same time.
Description
Technical field
The invention belongs to biological technical field, particularly, relate to the cultivation method of a kind of edible mushroom ladder rib hickory chick.
Background technology
Ladder rib hickory chick Morchella importuna M.Kuo, O ' Donnell & T.J.Volk, belongs to Ascomycotina Aseomycotina, discomycete Discomycetes, Pezizale Pezizales, Morchellaceae Morchellaceae, morchella Morchella.Mainly be distributed in Yunnan Province of China, Sichuan, Shaanxi, the ground such as California, USA, Nevada, the northwestward, Oregon and British Columbia, Canada.Fruit body is rich in amino acid and vitamin, and meat is tender and crisp, unique flavor, is food (medicine) one of treasure in bacterium.Morchella fungi is in Historical Evolution process, and due to dry morning, the fragmentation that desertization and mountain uplift etc. cause and geographic isolation, this genus fungi be there occurs, and Rapid radiating develops, foreign lands' one-tenth kind becomes to plant a phenomenon with ecological, thus creates abundant species.This genus fungi cultivation has a large amount of reported success, if (2008) such as Xu Zhongzhi are by inoculating Morchellaconica (M.conica) bacterial classification in host plants such as roundleaf poplars, again roundleaf poplar is transplanted to the slope of Rehabilitated land or soil depletion, strengthens Forest field management and can obtain the Morchellaconica fruit body (patent No.: 200810058482.0); Ower etc. (1989) are by Double-Mediums such as wheats, and after turning out sclerotium, induction produces hickory chick (Morchella sp.) fruit body (patent No.: us 4866878) again.But terraced rib hickory chick there is not yet the report of successfully cultivation of being correlated with.
Summary of the invention
The object of the present invention is to provide the cultivation method of a kind of terraced rib hickory chick, adopt the method plant terraced rib hickory chick, cost is low, operation is simple, the cycle is short, reproducible, be easy to manage and protect.
Above-mentioned purpose of the present invention is realized by following technical scheme:
The cultivation method of ladder rib hickory chick, comprise the making of terraced rib hickory chick bacterial classification, hydration-treated, planting material making, plant and manage and protect step, get by Molecular Identification be terraced rib hickory chick mother plant make cultivated species, cultivated species is after hydration-treated, broadcast sowing on whole face, railway carriage or compartment well, earthing, make and add planting material, field Routine Management 80-120 days, obtaining fruit body; Described terraced rib hickory chick being numbered in Genbank: JQ 618576, being numbered of female kind: HKAS 62868; Ladder rib hickory chick bacterial classification is the sclerotium sclerotia that typical bilayer medium culture obtains; Hydration-treated is that sclerotium processes 10-48 hour at 10-15 DEG C; Planting material adopts wood chip, rice husk or corncob 70-80%, wheat berry 5-10%, soil 10-18%, gypsum 1-2%, water content 50-65%; Plantation adopts solid spawn to be sown on face, wide 40-80cm alkalescence soil railway carriage or compartment, earthing 3-10cm, and by wide 40-60cm between face, railway carriage or compartment, dark 20-40cm manages ditch, by pouring water to just flooding face, railway carriage or compartment in ditch; Plantation after keep soil moisture be 55-70% to fruiting, keep after terraced rib hickory chick fruiting soil moisture 50-60%, air humidity 80-90% to results.
According to the cultivation method of described terraced rib hickory chick, wherein planting material adopts wood chip, rice husk or corncob 80%, wheat berry 3%, peat soil 15%, gypsum 2%, water content 65%, normal temperature and pressure sterilizing 20 hours.
According to the cultivation method of described terraced rib hickory chick, wherein soil is paddy soil or peat soil or cinnamon soil or chernozem or chestnut soil or brown soil or black mature soil or alpine meadow soil.
According to the cultivation method of described terraced rib hickory chick, wherein hydration-treated is that sclerotium processes 12-20 hour at 15 DEG C.
The invention provides that a kind of cost is low, operation is simple, the cycle is short, reproducible, be easy to the hickory chick cultivation method managed and protected; both increasing peasant income were promoted; be conducive to again the non-Foresty industry development model of ecological protection, simultaneously for hickory chick industrialized development provides a new cultivated strains.
Embodiment
By the following specific examples further illustrate the invention, but embodiment only for illustration of, can not the scope of the invention be limited.
Embodiment 1:
Carry out according to the following steps:
1, cultivated species makes: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) makes cultivated species, is the sclerotium (sclerotia) of typical bilayer medium culture acquisition.
2, hydration-treated is 15 DEG C, sclerotium process 12 hours.
3, planting material makes: wood chip 80%, wheat berry 5%, peat soil 14%, gypsum 1%, water content 65%, normal temperature and pressure sterilizing 20 hours.
4, implantation methods: bacterial classification is sown on face, wide 40cm railway carriage or compartment, earthing 5cm, wide 40cm between face, railway carriage or compartment, dark 40cm manages ditch, by pouring water in ditch to just flooding face, railway carriage or compartment.
5, method is managed and protected: after plantation, keep soil moisture 60% to fruiting; Soil moisture 55%, air humidity 80% to results are kept after fruiting.
Embodiment 2:
Carry out according to the following steps:
1, cultivated species makes: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) makes cultivated species, is the sclerotium (sclerotia) of typical bilayer medium culture acquisition.
2, hydration-treated is 10 DEG C, sclerotium process 24 hours.
3, planting material makes: wood chip 70%, wheat berry 10%, peat soil 18%, gypsum 2%, water content 60%, normal temperature and pressure goes out 20 hours.
4, implantation methods: bacterial classification is sown on face, wide 60cm railway carriage or compartment, earthing 8cm, wide 40cm between face, railway carriage or compartment, dark 40cm manages ditch, by pouring water in ditch to just flooding face, railway carriage or compartment.
5, method is managed and protected: after plantation, keep soil moisture 70% to fruiting; Soil moisture 55%, air humidity 90% to results are kept after fruiting.
Embodiment 3:
Carry out according to the following steps:
1, cultivated species makes: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) makes cultivated species, is the sclerotium (sclerotia) of typical bilayer medium culture acquisition.
2, hydration-treated is 15 DEG C, sclerotium process 24 hours.
3, planting material makes: rice husk 80%, wheat berry 5%, peat soil 14%, gypsum 1%, water content 65%, normal temperature and pressure sterilizing 20 hours.
4, implantation methods: bacterial classification is sown on face, wide 50cm railway carriage or compartment, earthing 3cm, wide 40cm between face, railway carriage or compartment, dark 40cm manages ditch, by pouring water in ditch to just flooding face, railway carriage or compartment.
5, method is managed and protected: after plantation, keep soil moisture 55% to fruiting; Soil moisture 50%, air humidity 90% to results are kept after fruiting.
Embodiment 4:
Carry out according to the following steps:
1, cultivated species makes: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) makes cultivated species, is the sclerotium (sclerotia) of typical bilayer medium culture acquisition.
2, hydration-treated is 10 DEG C, sclerotium process 36 hours.
3, planting material makes: rice husk 75%, wheat berry 8%, peat soil 15%, gypsum 2%, water content 60%, normal temperature and pressure sterilizing 20 hours.
4, implantation methods: bacterial classification is sown on face, wide 60cm railway carriage or compartment, earthing 5cm, wide 40cm between face, railway carriage or compartment, dark 40cm manages ditch, by pouring water in ditch to just flooding face, railway carriage or compartment.
5, method is managed and protected: after plantation, keep soil moisture 60% to fruiting; Soil moisture 55%, air humidity 85% to results are kept after fruiting.
Embodiment 5:
Carry out according to the following steps:
1, cultivated species makes: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) makes cultivated species, is the sclerotium (sclerotia) of typical bilayer medium culture acquisition.
2, hydration-treated is 15 DEG C, sclerotium process 48 hours.
3, planting material makes: corncob 73%, wheat berry 10%, peat soil 15%, gypsum 2%, water content 60%, normal temperature and pressure goes out 20 hours.
4, implantation methods: bacterial classification is sown on face, wide 60cm railway carriage or compartment, earthing 8cm, wide 40cm between face, railway carriage or compartment, dark 40cm manages ditch, by pouring water in ditch to just flooding face, railway carriage or compartment.
5, method is managed and protected: after plantation, keep soil moisture 70% to fruiting; Soil moisture 55%, air humidity 90% to results are kept after fruiting.
Claims (4)
1. the cultivation method of terraced rib hickory chick, it is characterized in that comprising the making of terraced rib hickory chick bacterial classification, hydration-treated, planting material making, planting and manage and protect step, get by Molecular Identification be terraced rib hickory chick mother plant make cultivated species, cultivated species is after hydration-treated, broadcast sowing on whole face, railway carriage or compartment well, earthing, make and add planting material, field Routine Management 80 ?120 days, obtain fruit body; Described terraced rib hickory chick being numbered in Genbank: JQ 618576, being numbered of female kind: HKAS 62868; Ladder rib hickory chick bacterial classification is the sclerotium sclerotia that typical bilayer medium culture obtains; Hydration-treated be sclerotium 10 ?process at 15 DEG C 10 ?48 hours; Planting material adopt wood chip, rice husk or corncob 70 ?80%, wheat berry 5 ?10%, soil 10 ?18%, gypsum 1 ?2%, water content 50 ?65%; Plantation adopt solid spawn be sown in wide 40 ?on face, 80cm alkalescence soil railway carriage or compartment, earthing 3 ?10cm, between face, railway carriage or compartment by wide 40 ?60cm, dark 20 ?40cm manage ditch, by pouring water to just flooding face, railway carriage or compartment in ditch; Keep after plantation soil moisture be 55-70% to fruiting, keep after terraced rib hickory chick fruiting soil moisture 50 ?60%, air humidity 80 ?90% to results.
2. the cultivation method of terraced rib hickory chick according to claim 1, is characterized in that planting material adopts wood chip, rice husk or corncob 80%, wheat berry 3%, peat soil 15%, gypsum 2%, water content 65%, normal temperature and pressure sterilizing 20 hours.
3. the cultivation method of terraced rib hickory chick according to claim 1, is characterized in that soil is paddy soil or peat soil or cinnamon soil or chernozem or chestnut soil or brown soil or black mature soil or alpine meadow soil.
4. the cultivation method of terraced rib hickory chick according to claim 1, it is characterized in that hydration-treated be sclerotium process at 15 DEG C 12 ?20 hours.
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| CN201310115468.0A CN103168622B (en) | 2013-04-03 | 2013-04-03 | Cultivating method of morchella importuna |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102663030B1 (en) * | 2019-12-11 | 2024-05-03 | 대한민국 | Medium composition for culturing morel and the culturing method for morel thereby |
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| CN104838883A (en) * | 2015-04-22 | 2015-08-19 | 吴中区胥口精益生物医药研究所 | Toadstool strain preparation process |
| CN105191667B (en) * | 2015-09-28 | 2018-02-06 | 秦小波 | Hickory chick nutrient formulation, nutrient bag and preparation method thereof and hickory chick cultural method |
| CN106613354A (en) * | 2016-12-28 | 2017-05-10 | 尤溪县林业科技推广中心 | Method for planting morchella importuna through mushroom residue |
| CN107253886A (en) * | 2017-06-01 | 2017-10-17 | 上海市农业科学院 | It is a kind of for nutrient bag of hickory chick and preparation method thereof |
| CN107371799A (en) * | 2017-06-01 | 2017-11-24 | 上海市农业科学院 | A kind of cultural method of terraced rib hickory chick |
| CN107409764B (en) * | 2017-09-29 | 2019-07-19 | 中国科学院昆明植物研究所 | A kind of ladder rib hickory chick autumn planting winter receipts cultural method |
| CN107455145B (en) * | 2017-09-29 | 2019-09-03 | 中国科学院昆明植物研究所 | A kind of cultivation management method of ladder rib hickory chick |
| CN109628626B (en) * | 2018-12-07 | 2022-07-26 | 东莞东阳光保健品研发有限公司 | Specific primer, kit and method for identifying morchella ladder and application of specific primer, kit and method |
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| US4594809A (en) * | 1985-04-29 | 1986-06-17 | Neogen Corporation | Cultivation of morchella |
| CN101283658A (en) * | 2008-06-02 | 2008-10-15 | 云南省农业科学院高山经济植物研究所 | Ecological cultivation method for spire morel |
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2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4594809A (en) * | 1985-04-29 | 1986-06-17 | Neogen Corporation | Cultivation of morchella |
| CN101283658A (en) * | 2008-06-02 | 2008-10-15 | 云南省农业科学院高山经济植物研究所 | Ecological cultivation method for spire morel |
Non-Patent Citations (4)
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| 尖顶羊肚菌仿生栽培技术;赵琪等;《西南农业学报》;20091231;第22卷(第6期);第1690-1693页 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102663030B1 (en) * | 2019-12-11 | 2024-05-03 | 대한민국 | Medium composition for culturing morel and the culturing method for morel thereby |
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