CN103168622A - Cultivating method of morchella importuna - Google Patents
Cultivating method of morchella importuna Download PDFInfo
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- CN103168622A CN103168622A CN2013101154680A CN201310115468A CN103168622A CN 103168622 A CN103168622 A CN 103168622A CN 2013101154680 A CN2013101154680 A CN 2013101154680A CN 201310115468 A CN201310115468 A CN 201310115468A CN 103168622 A CN103168622 A CN 103168622A
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Abstract
The invention discloses a cultivating method of morchella importuna. The cultivating method of the morchella importuna comprises a bacterium culturing producing step, a cultivation material producing step, a hydration processing step, a managing and protecting step and the like. A maternal species (HKAS 62868) which can be identified as the morchella importuna (the number of the Genbank is JQ 618576) through a molecular identification can be used for cultivating cultispecieses. After the hydration processing is carried out on the cultispecieses, the cultispecieses are sowed on the face of the right land and then are earthed up. The cultivation materials can be produced and added in the field and the field can be normally managed for 80-120 days, so that sporocarps can be obtained. The cultivating method of the morchella importuna aims at providing an edible mushroom cultivating method which not only is beneficial to the sustainable development of ecology, but also helps to increase farmer income and a novel cultivating bacterial strain is provided for the industrialization development of the morchella importuna at the same time.
Description
Technical field
The invention belongs to biological technical field, particularly, relate to the cultivation method of a kind of edible mushroom ladder rib hickory chick.
Background technology
Ladder rib hickory chick Morchella importuna M. Kuo, O ' Donnell ﹠amp; T.J. Volk, belong to Ascomycotina Aseomycotina, discomycete Discomycetes, Pezizale Pezizales, Morchellaceae Morchellaceae, morchella Morchella.Mainly be distributed in Yunnan Province of China, Sichuan, Shaanxi, the ground such as California, USA, Nevada, the northwestward, Oregon and British Columbia, Canada.Fruit body is rich in amino acid and vitamin, and meat is tender and crisp, and unique flavor is food (medicine) one of treasure in bacterium.The morchella fungi is in the Historical Evolution process, and owing to doing early, the fragmentation that desertization and mountain uplift etc. causes and geographic isolation make this genuss fungi that Rapid radiating evolution, foreign lands occur and become kind to become to plant a phenomenon with ecological, thereby produced the species that enrich.This belongs to, and fungi cultivation is existing successfully to be reported in a large number, pass through inoculation Morchellaconica (M. conica) bacterial classification in host plants such as roundleaf poplars as (2008) such as Xu Zhongzhi, again the roundleaf poplar is transplanted to the slope of concede the land to forestry ground or soil depletion, is strengthened the forest land management and can obtain the Morchellaconica fruit body (patent No.: 200810058482.0); Ower etc. (1989) are by Double-Mediums such as wheats, turn out to induce after sclerotium to produce hickory chick (Morchella sp.) fruit body (patent No.: us 4866878) again.But terraced rib hickory chick there is not yet the successfully report of cultivation of being correlated with.
Summary of the invention
The object of the present invention is to provide the cultivation method of a kind of terraced rib hickory chick, adopt the terraced rib hickory chick of the method plantation, cost is low, operation is simple, the cycle is short, good reproducibility, be easy to manage and protect.
Above-mentioned purpose of the present invention is to realize by following technical scheme:
The cultivation method of ladder rib hickory chick, comprise the making of terraced rib hickory chick bacterial classification, hydration-treated, cultivation bag making, plant and manage and protect step, mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank for JQ 618576) is made cultivated species, cultivated species is through after hydration-treated, broadcast sowing on whole railway carriage or compartment face well, earthing, make and add planting material, field Routine Management 80-120 days, obtaining fruit body.
In the cultivation method of described terraced rib hickory chick, rib hickory chick bacterial classification is that conventional method is cultivated the sclerotium sclerotia that obtains.
In the cultivation method of described terraced rib hickory chick, cultivation bag adopts wood chip, rice husk or corncob 70-80%, wheat berry 5-10%, soil 10-18%, gypsum 1-2%, water content 50-65%.
In the cultivation method of described terraced rib hickory chick, cultivation bag adopts wood chip, rice husk or corncob 80%, wheat berry 3%, and peat soil 15%, gypsum 2%, water content 65%, normal temperature and pressure went out 20 hours
In the cultivation method of described terraced rib hickory chick, soil is paddy soil, peat soil, cinnamon soil, chernozem, chestnut soil, brown soil, black mature soil, alpine meadow soil.
In the cultivation method of described terraced rib hickory chick, plantation adopts solid spawn to be sown on the face of wide 40-80cm railway carriage or compartment, earthing 3-10cm, and between the face of railway carriage or compartment, by wide 40-60cm, dark 20-40cm reason ditch is by pouring water in ditch to just flooding the railway carriage or compartment face.
In the cultivation method of described terraced rib hickory chick, hydration-treated is that sclerotium was processed 10-48 hour under 10-15 ℃.
In the cultivation method of described terraced rib hickory chick, hydration-treated is that sclerotium was processed 12-20 hour under 15 ℃.
In the cultivation method of described terraced rib hickory chick, keeping soil moisture after plantation is that 55-70% is to fruiting.
In the cultivation method of described terraced rib hickory chick, keep soil moisture 50-60%, air humidity 80-90% to results after terraced rib hickory chick fruiting.
The invention provides that a kind of cost is low, operation is simple, the cycle is short, good reproducibility, the hickory chick cultivation method that is easy to manage and protect; increasing peasant income had both been promoted; be conducive to again the non-forest products industry development model of ecological protection, provide a new cultivated strains for the hickory chick industrialized development simultaneously.
Embodiment
By the following specific examples further illustrate the invention, but embodiment only is used for explanation, can not limit the scope of the invention.
Embodiment 1:
Carry out according to the following steps:
1, cultivated species is made: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) is made cultivated species, is that conventional Double-Medium is cultivated the sclerotium (sclerotia) that obtains.
2, hydration-treated is that 15 ℃, sclerotium was processed 12 hours.
3, cultivation bag is made: wood chip 80%, wheat berry 5%, peat soil 14%, gypsum 1%, water content 65%, normal temperature and pressure sterilization 20 hours.
4, implantation methods: bacterial classification is sown on wide 40 railway carriage or compartment faces, earthing 5cm, and wide 40cm between the face of railway carriage or compartment, dark 40cm reason ditch is by pouring water in ditch to just flooding the railway carriage or compartment face.
5, manage and protect method: keep soil moisture 60% to fruiting after plantation; Keep soil moisture 55%, air humidity 80% to results after fruiting.
Embodiment 2:
Carry out according to the following steps:
1, cultivated species is made: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) is made cultivated species, is that conventional Double-Medium is cultivated the sclerotium (sclerotia) that obtains.
2, hydration-treated is that 10 ℃, sclerotium was processed 24 hours.
3, cultivation bag is made: wood chip 70%, and wheat berry 10%, peat soil 18%, gypsum 2%, water content 60%, normal temperature and pressure went out 20 hours.
4, implantation methods: bacterial classification is sown on wide 60 railway carriage or compartment faces, earthing 8cm, and wide 40cm between the face of railway carriage or compartment, dark 40cm reason ditch is by pouring water in ditch to just flooding the railway carriage or compartment face.
5, manage and protect method: keep soil moisture 70% to fruiting after plantation; Keep soil moisture 55%, air humidity 90% to results after fruiting.
Embodiment 3:
Carry out according to the following steps:
1, cultivated species is made: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) is made cultivated species, is that conventional Double-Medium is cultivated the sclerotium (sclerotia) that obtains.
2, hydration-treated is that 15 ℃, sclerotium was processed 24 hours.
3, cultivation bag is made: rice husk 80%, wheat berry 5%, peat soil 14%, gypsum 1%, water content 65%, normal temperature and pressure sterilization 20 hours.
4, implantation methods: bacterial classification is sown on wide 50 railway carriage or compartment faces, earthing 3cm, and wide 40cm between the face of railway carriage or compartment, dark 40cm reason ditch is by pouring water in ditch to just flooding the railway carriage or compartment face.
5, manage and protect method: keep soil moisture 55% to fruiting after plantation; Keep soil moisture 50%, air humidity 90% to results after fruiting.
Embodiment 4:
Carry out according to the following steps:
1, cultivated species is made: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) is made cultivated species, is that conventional Double-Medium is cultivated the sclerotium (sclerotia) that obtains.
2, hydration-treated is that 10 ℃, sclerotium was processed 36 hours.
3, cultivation bag is made: rice husk 75%, wheat berry 8%, peat soil 15%, gypsum 2%, water content 60%, normal temperature and pressure sterilization 20 hours.
4, implantation methods: bacterial classification is sown on wide 60 railway carriage or compartment faces, earthing 5cm, and wide 40cm between the face of railway carriage or compartment, dark 40cm reason ditch is by pouring water in ditch to just flooding the railway carriage or compartment face.
5, manage and protect method: keep soil moisture 60% to fruiting after plantation; Keep soil moisture 55%, air humidity 85% to results after fruiting.
Embodiment 5:
Carry out according to the following steps:
1, cultivated species is made: mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank be JQ 618576) is made cultivated species, is that conventional Double-Medium is cultivated the sclerotium (sclerotia) that obtains.
2, hydration-treated is that 15 ℃, sclerotium was processed 48 hours.
3, cultivation bag is made: corncob 73%, and wheat berry 10%, peat soil 15%, gypsum 2%, water content 60%, normal temperature and pressure went out 20 hours.
4, implantation methods: bacterial classification is sown on wide 60 railway carriage or compartment faces, earthing 8cm, and wide 40cm between the face of railway carriage or compartment, dark 40cm reason ditch is by pouring water in ditch to just flooding the railway carriage or compartment face.
5, manage and protect method: keep soil moisture 70% to fruiting after plantation; Keep soil moisture 55%, air humidity 90% to results after fruiting.
Claims (10)
1. the cultivation method of terraced rib hickory chick, it is characterized in that comprising the making of terraced rib hickory chick bacterial classification, hydration-treated, cultivation bag making, plant and manage and protect step, mother's kind (HKAS 62868) that to get by Molecular Identification be terraced rib hickory chick (No. Genbank for JQ 618576) is made cultivated species, cultivated species is through after hydration-treated, broadcast sowing on whole railway carriage or compartment face well, earthing, make and add planting material, field Routine Management 80-120 days, obtaining fruit body.
2. the cultivation method of terraced rib hickory chick according to claim 1, is characterized in that terraced rib hickory chick bacterial classification is that conventional method is cultivated the sclerotium sclerotia that obtains.
3. the cultivation method of terraced rib hickory chick according to claim 1, is characterized in that cultivation bag adopts wood chip, rice husk or corncob 70-80%, wheat berry 5-10%, soil 10-18%, gypsum 1-2%, water content 50-65%.
4. the cultivation method of terraced rib hickory chick according to claim 3, is characterized in that cultivation bag adopts wood chip, rice husk or corncob 80%, wheat berry 3%, and peat soil 15%, gypsum 2%, water content 65%, normal temperature and pressure went out 20 hours.
5. the cultivation method of terraced rib hickory chick according to claim 3, is characterized in that soil is paddy soil, peat soil, cinnamon soil, chernozem, chestnut soil, brown soil, black mature soil, alpine meadow soil.
6. the cultivation method of terraced rib hickory chick according to claim 1 is characterized in that plantation adopts solid spawn to be sown on the face of wide 40-80cm railway carriage or compartment, earthing 3-10cm, and between the face of railway carriage or compartment, by wide 40-60cm, dark 20-40cm reason ditch is by pouring water in ditch to just flooding the railway carriage or compartment face.
7. the cultivation method of terraced rib hickory chick according to claim 1, is characterized in that hydration-treated is that sclerotium was processed 10-48 hour under 10-15 ℃.
8. the cultivation method of terraced rib hickory chick according to claim 7, is characterized in that hydration-treated is that sclerotium was processed 12-20 hour under 15 ℃.
9. the cultivation method of terraced rib hickory chick according to claim 1, its feature keeps soil moisture after plantation be that 55-70% is to fruiting.
10. the cultivation method of according to claim 1 or 9 described terraced rib hickory chicks, is characterized in that keeping soil moisture 50-60%, air humidity 80-90% to results after terraced rib hickory chick fruiting.
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| CN201310115468.0A CN103168622B (en) | 2013-04-03 | 2013-04-03 | Cultivating method of morchella importuna |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104838883A (en) * | 2015-04-22 | 2015-08-19 | 吴中区胥口精益生物医药研究所 | Toadstool strain preparation process |
| CN105191667A (en) * | 2015-09-28 | 2015-12-30 | 四川省自然资源科学研究院 | Morchella nutrition formula, nutrition bag, preparation method of nutrition bag and morchella culture method |
| CN106613354A (en) * | 2016-12-28 | 2017-05-10 | 尤溪县林业科技推广中心 | Method for planting morchella importuna through mushroom residue |
| CN107253886A (en) * | 2017-06-01 | 2017-10-17 | 上海市农业科学院 | It is a kind of for nutrient bag of hickory chick and preparation method thereof |
| CN107371799A (en) * | 2017-06-01 | 2017-11-24 | 上海市农业科学院 | A kind of cultural method of terraced rib hickory chick |
| CN107409764A (en) * | 2017-09-29 | 2017-12-01 | 中国科学院昆明植物研究所 | A kind of terraced rib hickory chick autumn planting winter receives cultural method |
| CN107455145A (en) * | 2017-09-29 | 2017-12-12 | 中国科学院昆明植物研究所 | A kind of cultivation management method of terraced rib hickory chick |
| CN109628626A (en) * | 2018-12-07 | 2019-04-16 | 广东东阳光药业有限公司 | Identify specific primer, the kit, method and its application of terraced rib hickory chick |
| KR20230106555A (en) * | 2019-12-11 | 2023-07-13 | 대한민국(농촌진흥청장) | Medium composition for culturing morel and the culturing method for morel thereby |
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| US4594809A (en) * | 1985-04-29 | 1986-06-17 | Neogen Corporation | Cultivation of morchella |
| CN101283658A (en) * | 2008-06-02 | 2008-10-15 | 云南省农业科学院高山经济植物研究所 | Ecological cultivation method for spire morel |
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| US4594809A (en) * | 1985-04-29 | 1986-06-17 | Neogen Corporation | Cultivation of morchella |
| CN101283658A (en) * | 2008-06-02 | 2008-10-15 | 云南省农业科学院高山经济植物研究所 | Ecological cultivation method for spire morel |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104838883A (en) * | 2015-04-22 | 2015-08-19 | 吴中区胥口精益生物医药研究所 | Toadstool strain preparation process |
| CN105191667A (en) * | 2015-09-28 | 2015-12-30 | 四川省自然资源科学研究院 | Morchella nutrition formula, nutrition bag, preparation method of nutrition bag and morchella culture method |
| CN105191667B (en) * | 2015-09-28 | 2018-02-06 | 秦小波 | Hickory chick nutrient formulation, nutrient bag and preparation method thereof and hickory chick cultural method |
| CN106613354A (en) * | 2016-12-28 | 2017-05-10 | 尤溪县林业科技推广中心 | Method for planting morchella importuna through mushroom residue |
| CN107253886A (en) * | 2017-06-01 | 2017-10-17 | 上海市农业科学院 | It is a kind of for nutrient bag of hickory chick and preparation method thereof |
| CN107371799A (en) * | 2017-06-01 | 2017-11-24 | 上海市农业科学院 | A kind of cultural method of terraced rib hickory chick |
| CN107455145A (en) * | 2017-09-29 | 2017-12-12 | 中国科学院昆明植物研究所 | A kind of cultivation management method of terraced rib hickory chick |
| CN107409764A (en) * | 2017-09-29 | 2017-12-01 | 中国科学院昆明植物研究所 | A kind of terraced rib hickory chick autumn planting winter receives cultural method |
| CN107409764B (en) * | 2017-09-29 | 2019-07-19 | 中国科学院昆明植物研究所 | A kind of ladder rib hickory chick autumn planting winter receipts cultural method |
| CN107455145B (en) * | 2017-09-29 | 2019-09-03 | 中国科学院昆明植物研究所 | A kind of cultivation management method of ladder rib hickory chick |
| CN109628626A (en) * | 2018-12-07 | 2019-04-16 | 广东东阳光药业有限公司 | Identify specific primer, the kit, method and its application of terraced rib hickory chick |
| CN109628626B (en) * | 2018-12-07 | 2022-07-26 | 东莞东阳光保健品研发有限公司 | Specific primer, kit and method for identifying morchella ladder and application of specific primer, kit and method |
| KR20230106555A (en) * | 2019-12-11 | 2023-07-13 | 대한민국(농촌진흥청장) | Medium composition for culturing morel and the culturing method for morel thereby |
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| CN103168622B (en) | 2015-02-25 |
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