CN103163106B - 一种基于受激发射损耗的超分辨荧光寿命成像方法和装置 - Google Patents
一种基于受激发射损耗的超分辨荧光寿命成像方法和装置 Download PDFInfo
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- CN103163106B CN103163106B CN201310039978.4A CN201310039978A CN103163106B CN 103163106 B CN103163106 B CN 103163106B CN 201310039978 A CN201310039978 A CN 201310039978A CN 103163106 B CN103163106 B CN 103163106B
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
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CN201310039978.4A CN103163106B (zh) | 2013-01-30 | 2013-01-30 | 一种基于受激发射损耗的超分辨荧光寿命成像方法和装置 |
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Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103543135B (zh) * | 2013-10-18 | 2016-06-01 | 浙江大学 | 一种基于荧光寿命分布的纳米精度光斑对准方法和装置 |
DE102015105018A1 (de) * | 2015-03-31 | 2016-10-06 | Laser-Laboratorium Göttingen e.V. | Verfahren und Rasterfluoreszenzlichtmikroskop zum mehrdimensional hochauflösenden Abbilden einer Struktur oder eines Wegs eines Partikels in einer Probe |
CN105044066B (zh) * | 2015-08-07 | 2017-10-13 | 浙江大学 | 一种基于宽带受激辐射的纳米oct成像方法及*** |
CN106645064B (zh) * | 2016-12-13 | 2019-10-18 | 华南师范大学 | 一种受激辐射损耗方法、超分辨成像方法及显微成像装置 |
CN107121362A (zh) * | 2017-04-17 | 2017-09-01 | 金华职业技术学院 | 一种研究分子迁移运动的方法 |
CN107290322B (zh) * | 2017-07-25 | 2020-04-14 | 潍坊学院 | 一种基于时间分辨荧光光谱确定石油包裹体成藏关键时刻的装置及方法 |
CN107860756A (zh) * | 2017-11-12 | 2018-03-30 | 武汉能斯特科技有限公司 | 一种测量荧光寿命的方法和装置 |
CN108333157B (zh) * | 2018-01-23 | 2021-08-03 | 深圳大学 | 生物分子三维动态分析方法和*** |
CN108627484B (zh) * | 2018-04-26 | 2020-05-19 | 华中科技大学 | 一种自动锁相模块及荧光寿命检测方法和装置 |
CN109713565B (zh) * | 2019-02-03 | 2020-05-26 | 浙江大学 | 波长快速调谐的半导体纳米线激光器 |
CN112485230B (zh) * | 2019-09-11 | 2022-03-18 | 复旦大学 | 基于主动时间调制混频激发照射的超分辨显微成像方法及装置 |
CN110907414A (zh) * | 2019-11-01 | 2020-03-24 | 浙江大学 | 一种基于并行探测的二维亚十纳米定位方法及装置 |
CN114812402B (zh) * | 2022-05-20 | 2023-12-26 | 南昌航空大学 | 一种同轴位移检测的光学方法 |
WO2024060018A1 (en) * | 2022-09-20 | 2024-03-28 | Juan Liu | Non-invasive method for detection, visualization and/or quantification of an endogenous fluorophore such as melanin in a biological tissue |
Citations (4)
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CN101632577A (zh) * | 2009-08-20 | 2010-01-27 | 浙江大学 | 基于频域荧光寿命成像的牙釉质矿物质含量检测的方法和装置 |
CN102033058A (zh) * | 2010-11-19 | 2011-04-27 | 深圳大学 | 一种超分辨荧光寿命成像方法及成像*** |
WO2012069076A1 (en) * | 2010-11-22 | 2012-05-31 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Sted microscopy with pulsed excitation, continuous stimulation, and gated registration of spontaneously emitted fluorescence light |
CN102692401A (zh) * | 2012-06-06 | 2012-09-26 | 中国科学院半导体研究所 | 基于光延迟的门控荧光寿命成像装置 |
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DE102006047816A1 (de) * | 2006-10-07 | 2008-04-17 | Leica Microsystems Cms Gmbh | Verfahren und Vorrichtung zum hochaufgelösten optischen Abtasten einer Probe |
DE102007039111B4 (de) * | 2007-08-18 | 2014-11-20 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | STED-Fluoreszenzmikroskopie mit Zweiphotonen-Anregung |
US20100251438A1 (en) * | 2009-03-25 | 2010-09-30 | The Royal College Of Surgeons In Ireland | Microscopy control system and method |
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Patent Citations (4)
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CN101632577A (zh) * | 2009-08-20 | 2010-01-27 | 浙江大学 | 基于频域荧光寿命成像的牙釉质矿物质含量检测的方法和装置 |
CN102033058A (zh) * | 2010-11-19 | 2011-04-27 | 深圳大学 | 一种超分辨荧光寿命成像方法及成像*** |
WO2012069076A1 (en) * | 2010-11-22 | 2012-05-31 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Sted microscopy with pulsed excitation, continuous stimulation, and gated registration of spontaneously emitted fluorescence light |
CN102692401A (zh) * | 2012-06-06 | 2012-09-26 | 中国科学院半导体研究所 | 基于光延迟的门控荧光寿命成像装置 |
Non-Patent Citations (4)
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Enhance the resolution of STED by fluorescent lifetime subtractive imaging;Xiang Hao et al.;《2012 Asia Communications and Photonics Conference》;20121110;1-3 * |
Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging;Xiang Hao et al.;《OPTICS LETTERS》;20080115;第33卷(第2期);113-115 * |
Supercontinuum Stimulated Emission Depletion Fluorescence Lifetime Imaging;Michael D. Lesoine et al.;《The Journal of Physical Chemistry B》;20120712;第116卷(第27期);7821-7826 * |
基于时间相关单光子计数的离线式g-STED超分辨显微术;郝翔等;《中国激光》;20130110;第40卷(第1期);0104001-1-0104001-5 * |
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