CN103145462A - Enoki mushroom stock culture material and making method thereof - Google Patents
Enoki mushroom stock culture material and making method thereof Download PDFInfo
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- CN103145462A CN103145462A CN2013100913782A CN201310091378A CN103145462A CN 103145462 A CN103145462 A CN 103145462A CN 2013100913782 A CN2013100913782 A CN 2013100913782A CN 201310091378 A CN201310091378 A CN 201310091378A CN 103145462 A CN103145462 A CN 103145462A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention relates to an enoki mushroom stock culture material. The enoki mushroom stock culture material is characterized in that broad bean seed coats (not bean pod) are taken as a main raw material, sawdust and rice bran are taken as auxiliary raw materials, and the water content of the culture material is 60-63%. The invention further provides a making method of the culture material. The enoki mushroom stock culture material and the making method of the culture material disclosed by the invention have the advantages that (1) the channel of raw materials is widened, and the cost is reduced; (2) the culture material prepared by the formula in the invention keeps excellent air permeability and also has enhanced water sucking and retaining capacities, and through the culture material, the fungus growth is quick and the hybridization resistance is strong; and (3) when cultivated species prepared by the stock are applied to production, the yield is high and the quality is excellent. The invention has the following social benefits that the broad bean seed coats replace the sawdust so that part of the fungus-forest contradiction can be released, the waste is turned into the wealth, and the environment protection is facilitated.
Description
Technical field
Content of the present invention comprises needle mushroom Primary spawn material compatibility, and the making method of this Primary spawn material, belongs to edible fungus species and makes the field.
Background technology
Needle mushroom has another name called dried mushroom, because its stem shape and the color and luster utmost point resemble Flos Hemerocallis, therefore claim needle mushroom.Needle mushroom is sliding tender delicious, nutritious, contains 18 seed amino acids, and the amino acid total content is up to 20.9%, and wherein, 44.5% is again necessary 8 seed amino acids of human body, apparently higher than general mushroom class; Due to bad amino acid and arginine content also higher, therefore, often edible needle mushroom is also very favourable to the growth of children brain cell.
The weak wooden saprophytic microorganism of genus flammulina, all the time, the mushroom agriculture is all to make the needle mushroom original seed take wood chip as main raw material.But nowadays, " bacterium-Lin " contradiction, so each production unit is all attached great importance to looking for cheap and good-quality alternative materials.
Broad bean is at China's cultivation history of existing more than 2000.There is plantation in most of provinces to broad bean in the whole nation, and the areas to the south, the Changjiang river is take autumn sowing winter kind as main, and North of Yangtze River is early to sow in spring as main.
Over nearly 10 years, Chinese broad bean is produced area and is reached more than 130 ten thousand hectares, accounts for 45.7% of world's total area, is the first in the world.
Broad bean beans meat is the main raw material of brewery, and broad bean kind skin is the tankage of brewery.At present, broad bean kind skin is except a part is used as fuel, and it is discarded that major part all is taken as rubbish.
But in fact, broad bean kind skin contains a large amount of xylogen, Mierocrystalline cellulose and hemicellulose, is comparatively ideal edible fungus culturing raw material.
Along with the growth of broad bean processing industry, broad bean kind skin quantity also increases sharply in recent years, therefore, utilizes these tankage to be not only this behave of falling of an Edible Fungi, but also is a circular agriculture and environmental protection cause behave.
Summary of the invention
The present invention has following two aspects:
1, the needle mushroom Primary spawn material compatibility take broad bean kind skin as main raw material.
2, the making method of broad bean kind skin Primary spawn material.
The technical solution adopted in the present invention is:
A kind of needle mushroom Primary spawn material compatibility is characterized in that the component of this culture material has following quality proportioning: broad bean kind skin 50%-60%, wood chip 10%-20%, rice bran 30%.
Broad bean kind skin through drying, is ground into 4mm following (particle diameter), and the main component of broad bean kind skin is Mierocrystalline cellulose, hemicellulose, xylogen, still contains in addition certain mineral substance, protein, VITAMIN etc.Wherein Mierocrystalline cellulose 56.8%, hemicellulose 11.8%, xylogen 8.7%, crude protein 4.64%, structural protein 4.12%.
Wood chip is the weed tree sawdust that ferments more than half a year.
Rice bran is commercially available.
A kind of making method of needle mushroom Primary spawn material is characterized in that comprising the steps:
1. the culture material fermentation of banking up: first broad bean kind skin and wood chip are mixed under drying regime and turned over evenly, thereafter these compounds are prewetted (fully drenched), then wet feed is piled high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material of every heap is controlled at dry weight 250kg-500kg, after carrying out heap, surrounding is patted, then insert some vertical pores with the wooden stick of diameter 10cm-15cm on heap, at the bottom of straight through reactor, stomatal frequency is 1/m
3, cover at last straw mat by its spontaneous fermentation;
2. turning: after material temperature reaches 60 ℃, keep 24h, then carry out turning, and later on turning every day once, adds rice bran when turning for the second time, and the water content of looking windrow keeps the skin wet, the turning number of times is 3-4 time altogether, and when stopping fermentation, the water content of culture material is 60%-63%;
3. bottle, beat and plant the bacterium hole: with the fermentation material 1100ml * 78mmPP culturing bottle of packing into, stamp and plant the bacterium hole, the aperture of planting the bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;
4. sterilization, cooling: culturing bottle is carried out autoclaving, first keep 100 ℃ of sterilization 1h, then rise to 121 ℃ of autoclaving 1.5h; Process of cooling is: naturally cool to below 25 ℃.
Culturing bottle is after the female kind of access, and culturing room's temperature is controlled at 16 ℃～18 ℃, and relative air humidity is controlled at 60%～75%; Through the cultivation of 20d-22d, mycelia is covered with bottle, namely can be used as to make the cultivar use.
The novel method of utilizing broad bean kind leather to make the needle mushroom original seed provided by the invention has the advantage of three aspects:: the one, widened the raw material channel, and reduced cost; The 2nd, the culture material made of compatibility, both kept good air permeability thus, strengthened again the water-absorbing-retaining ability, and the culture material performance is set out, and bacterium is fast, anti-assorted power is strong; The 3rd, the cultivar that utilizes this original seed to make is applied to produce, and shows high yield and high quality.Social benefit of the present invention is, substitutes wood chip with broad bean kind skin and can relax a part " bacterium-Lin " contradiction, and turn waste into wealth, be beneficial to environmental protection.
Embodiment
Mode I: the proportioning of culture material dry-matter: broad bean kind skin 52%, wood chip 18%, rice bran 30%.
Making method: with broad bean kind skin and wood chip is dried prewets after turning over evenly, after it is piled high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material of every heap is controlled at dry weight 250kg-500kg, after carrying out heap, surrounding is patted, then insert some vertical pores with the wooden stick of diameter 10cm-15cm on heap, at the bottom of straight through reactor, stomatal frequency is 1/m
3, then cover straw mat by its spontaneous fermentation.Keep 24h to get final product turning after material temperature reaches 60 ℃, later turning every day once adds rice bran when turning for the second time, and looks the windrow humidity and adjust moisture, and turning is 3-4 time altogether, and after stopping fermentation, the water content of culture material is controlled 60%-63%; Pack in 1100ml * 78mmPP culture bottle with bottling machine, bottling requires to accomplish to tighten lower pine, every bottled culture material 730g (wet feed) stamps and plants the bacterium hole, and the aperture is 16mm, then cover the bottle cap of the ventilative sponge of band, put into the autoclave sterilization, first make 100 ℃/1h and process, then make 121 ℃/1.5h and process, naturally cool to after sterilization below 25 ℃, can inoculate female the kind.
After inoculation, inoculation bottle is moved in culturing room, culturing room requires dark, and temperature is controlled at 16 ℃～18 ℃, and relative air humidity is controlled at 60%～75%.Cultivate through 20d-22d, cover with mycelia in bottle.Utilize original seed that the present embodiment culture material is made to send out bacterium fast, anti-assorted power is strong, and the cultivar of making of this original seed is applied to production, there is no significant difference with the output of control group.
Mode II: the proportioning of culture material dry-matter: broad bean kind skin 55%, wood chip 15%, rice bran 30%.Making method is with reference to mode I.Utilize original seed that the present embodiment culture material is made to send out bacterium fast, anti-assorted power is strong, and the cultivar of making of this original seed is applied to production, there is no significant difference with the output of control group.
Mode III: the proportioning of culture material dry-matter: broad bean kind skin 58%, wood chip 12%, rice bran 30%.Making method is with reference to mode I.Utilize original seed that the present embodiment culture material is made to send out bacterium fast, anti-assorted power is strong, and the cultivar of making of this original seed is applied to production, there is no significant difference with the output of control group.
Mode IV: the manner is control group, and the proportioning of its culture material dry-matter is: wood chip 70%, rice bran 30%.
The original seed mycelial growth of different ingredients and be applied to produce rear output information slip
Annotate: mycelial density with ++, +++and ++ ++ expression ,+number more multilist shows that mycelial density is higher.
Claims (4)
1. needle mushroom Primary spawn material, its component and quality proportioning are:
Broad bean kind skin 50%-60%, wood chip 10%-20%, rice bran 30%.
2. needle mushroom Primary spawn material according to claim 1, it is characterized in that: the particle diameter of described broad bean kind skin is controlled at below 4mm.
3. the making method of the needle mushroom Primary spawn material described according to claim 1 is characterized in that:
1. the fermentation of banking up: first broad bean kind skin and wood chip are mixed under drying regime and turned over evenly, thereafter these compounds are prewetted (fully drenched), then wet feed is piled high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material of every heap is controlled at dry weight 250kg-500kg, after carrying out heap, surrounding is patted, then insert some vertical pores with the wooden stick of diameter 10cm-15cm on heap, at the bottom of straight through reactor, stomatal frequency is 1/m
3, cover at last straw mat by its spontaneous fermentation;
2. turning: after material temperature reaches 60 ℃, keep 24h, then carry out turning, and later on turning every day once, adds rice bran when turning for the second time, and the water content of looking windrow keeps the skin wet, the turning number of times is 3-4 time altogether, time remaining 5d-6d, when stopping fermentation, the water content of culture material is 60%-63%;
3. bottle, beat and plant the bacterium hole: with the fermentation material 1100ml * 78mmPP culturing bottle of packing into, stamp and plant the bacterium hole, the aperture of planting the bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;
4. sterilization, cooling: culturing bottle is carried out autoclaving, first keep 100 ℃ of sterilization 1h, then rise to 121 ℃ of autoclaving 1.5h; Process of cooling is: naturally cool to below 25 ℃.
4. utilize the making method of the needle mushroom Primary spawn material described in claim 1-3, it is characterized in that: needle mushroom Primary spawn material is after the female kind of access, culturing room's temperature is controlled at 16 ℃～18 ℃, relative air humidity is controlled at 60%～75%, cultivation through 20d-22d, mycelia is covered with bottle, namely can be used as to make the cultivar use.
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Cited By (17)
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|---|---|---|---|---|
| CN103304337A (en) * | 2013-06-24 | 2013-09-18 | 邬金飞 | Formula for agaricus blazei murill stock culture material and method for preparing culture material |
| CN103304335A (en) * | 2013-06-23 | 2013-09-18 | 邬金飞 | Formula for agaricus blazei murill stock culture material and method for preparing culture material |
| CN103304336A (en) * | 2013-06-20 | 2013-09-18 | 邬金飞 | Formula for coprinus comatus stock culture material and method for preparing culture material |
| CN103319259A (en) * | 2013-06-23 | 2013-09-25 | 邬金飞 | Formula of mushroom stock compost and manufacturing method of compost |
| CN103387448A (en) * | 2013-07-07 | 2013-11-13 | 邬金飞 | Golden needle mushroom cultivation material combination and production method thereof |
| CN103396231A (en) * | 2013-07-21 | 2013-11-20 | 邬金飞 | Culture material composition for straw mushroom breeder seeds, and preparation method of culture material |
| CN103396230A (en) * | 2013-07-23 | 2013-11-20 | 邬金飞 | Formula and manufacturing method of Pleurotus citrinopileatus culture material |
| CN103435408A (en) * | 2013-08-06 | 2013-12-11 | 邬金飞 | Pholiota nameko cultivation material compatibility and method for manufacturing cultivation material |
| CN104151086A (en) * | 2014-09-01 | 2014-11-19 | 四川大自然惠川食品有限公司 | Culture medium and culture method of flammulina velutiper |
| CN104671930A (en) * | 2013-11-28 | 2015-06-03 | 重庆市南川区南双科技有限公司 | Method for producing soilless culture medium for needle mushrooms |
| CN104725115A (en) * | 2015-02-25 | 2015-06-24 | 邬金梅 | Preparation method of flammulina velutipes cultivation material |
| CN104725116A (en) * | 2015-02-25 | 2015-06-24 | 邬金梅 | Preparation method of flammulina velutipes cultivation material |
| CN104725117A (en) * | 2015-02-25 | 2015-06-24 | 邬金梅 | Preparation method of flammulina velutipes cultivation material |
| CN104744158A (en) * | 2015-03-08 | 2015-07-01 | 邬金梅 | Method for manufacturing flammulina velutipes compost |
| CN104744159A (en) * | 2015-03-08 | 2015-07-01 | 邬金梅 | Method for manufacturing flammulina velutipes compost |
| CN109392596A (en) * | 2018-12-03 | 2019-03-01 | 贵州家鑫食用菌有限公司 | A method of green grass bacterium is cultivated using clinker |
| CN109392599A (en) * | 2018-12-03 | 2019-03-01 | 贵州家鑫食用菌有限公司 | A method of green grass bacterium is planted using fermentation material |
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| CN103304336A (en) * | 2013-06-20 | 2013-09-18 | 邬金飞 | Formula for coprinus comatus stock culture material and method for preparing culture material |
| CN103304336B (en) * | 2013-06-20 | 2015-03-25 | 邬金飞 | Formula for coprinus comatus stock culture material and method for preparing culture material |
| CN103319259B (en) * | 2013-06-23 | 2014-12-24 | 邬金飞 | Formula of mushroom stock compost and manufacturing method of compost |
| CN103304335A (en) * | 2013-06-23 | 2013-09-18 | 邬金飞 | Formula for agaricus blazei murill stock culture material and method for preparing culture material |
| CN103319259A (en) * | 2013-06-23 | 2013-09-25 | 邬金飞 | Formula of mushroom stock compost and manufacturing method of compost |
| CN103304337A (en) * | 2013-06-24 | 2013-09-18 | 邬金飞 | Formula for agaricus blazei murill stock culture material and method for preparing culture material |
| CN103387448A (en) * | 2013-07-07 | 2013-11-13 | 邬金飞 | Golden needle mushroom cultivation material combination and production method thereof |
| CN103387448B (en) * | 2013-07-07 | 2015-02-25 | 邬金飞 | Golden needle mushroom cultivation material combination and production method thereof |
| CN103396231A (en) * | 2013-07-21 | 2013-11-20 | 邬金飞 | Culture material composition for straw mushroom breeder seeds, and preparation method of culture material |
| CN103396230B (en) * | 2013-07-23 | 2014-12-24 | 邬金飞 | Formula and manufacturing method of Pleurotus citrinopileatus culture material |
| CN103396230A (en) * | 2013-07-23 | 2013-11-20 | 邬金飞 | Formula and manufacturing method of Pleurotus citrinopileatus culture material |
| CN103435408A (en) * | 2013-08-06 | 2013-12-11 | 邬金飞 | Pholiota nameko cultivation material compatibility and method for manufacturing cultivation material |
| CN103435408B (en) * | 2013-08-06 | 2015-04-22 | 邬金飞 | Pholiota nameko cultivation material compatibility and method for manufacturing cultivation material |
| CN104671930A (en) * | 2013-11-28 | 2015-06-03 | 重庆市南川区南双科技有限公司 | Method for producing soilless culture medium for needle mushrooms |
| CN104151086A (en) * | 2014-09-01 | 2014-11-19 | 四川大自然惠川食品有限公司 | Culture medium and culture method of flammulina velutiper |
| CN104725115A (en) * | 2015-02-25 | 2015-06-24 | 邬金梅 | Preparation method of flammulina velutipes cultivation material |
| CN104725116A (en) * | 2015-02-25 | 2015-06-24 | 邬金梅 | Preparation method of flammulina velutipes cultivation material |
| CN104725117A (en) * | 2015-02-25 | 2015-06-24 | 邬金梅 | Preparation method of flammulina velutipes cultivation material |
| CN104744158A (en) * | 2015-03-08 | 2015-07-01 | 邬金梅 | Method for manufacturing flammulina velutipes compost |
| CN104744159A (en) * | 2015-03-08 | 2015-07-01 | 邬金梅 | Method for manufacturing flammulina velutipes compost |
| CN109392596A (en) * | 2018-12-03 | 2019-03-01 | 贵州家鑫食用菌有限公司 | A method of green grass bacterium is cultivated using clinker |
| CN109392599A (en) * | 2018-12-03 | 2019-03-01 | 贵州家鑫食用菌有限公司 | A method of green grass bacterium is planted using fermentation material |
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