CN103141702A - Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea - Google Patents
Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea Download PDFInfo
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Abstract
The invention discloses a Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea, and belongs to the field of feed additives. The feed additive is prepared from the main materials of snow grass meal, curcuma powder, semen cassiae powder, burdock powder, shallot white powder, Chinese herbal medicine preparation and curcuma extract, and toadstool and bacillus subtilis are scientifically compounded, so that the application amount of antibiotics medicine in cultivation of pigs is effectively reduced, the use amount of the antibiotic is reduced by above 60%, the drug property of the Chinese herbal medicine is improved, the porcine epizootic diarrhea caused by porcine epidemic diarrhea virus can be effectively prevented and treated, and the Chinese herb extract feed additive is safe and non-toxic. Besides, the amount of the additive in the feed is low, the quality is stable, an acting effect is improved by combining different Chinese herb extracts, the porcine epizootic diarrhea is reduced by 60-80%, and the taste and quality of animal products are obviously improved. A sense experimental evaluation proves that the evaluation score on the taste and quality of the animal products using the additive is improved by above 65%.
Description
Technical field:
The invention belongs to feed additive field, particularly a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea.
Background technology:
Pig epidemic diarrhea (porcine epidemic diarrhea, PED) be by Porcine epidemic diarrhea virus (porcine epidemic diarrhea virus, PEDV) cause take pig watery diarrhea, vomiting, dehydration with to a kind of height contact enteric infectious disease of the high fatal rate of suckling pig as principal character.Porcine epidemic diarrhea virus belongs to cover virales coronaviridae coronavirus genus.Virion is polymorphy, be inclined to spherical, diameter 95～l90nm.Cyst membrane is arranged outward, the petal-shaped fiber initiation is arranged on cyst membrane, Porcine epidemic diarrhea virus is nucleic acid based is the RNA type.The full member of coronavirus genus is classified PEDV as in the 6th report of nineteen ninety-five ICTV (CTV).At first this disease has reported the generation of PEDV in Britain from 1971, in succession reported the generation of this disease in many countries such as Belgium, Germany, Switzerland, Japan, between in the past 30 years, the infection of PEDV in the pig farm, Asia is very serious, and China is in the generation of reported first PED in 1976.In recent years; this sick epidemic regions has the trend that enlarges gradually; from winter in 2010 so far; the Asian countries such as Thailand, Philippine, Korea S; and a lot of large-scale pig farms in most of province of China; suckling pig occurs successively acutely suffer from diarrhoea, dead serious case has caused huge economic loss for China's pig industry.
How to solve that in the industry of raising pigs at present, ubiquitous pig epidemic diarrhea is a major issue, Chinese herbal medicine is nontoxic because of it, have no side effect, the resistance to the action of a drug is strong etc., and advantage becomes the feed addictive new lover, but mostly use now single Chinese herbal medicine, act on singlely, the resistance to the action of a drug is single; In order to reach the purpose that prevents disease, directly use Chinese herbal medicine, its use amount is larger, affects the feed mouthfeel, and quality index is difficult to control.Therefore the feed addictive that adopts multiple Chinese herbal medicine preparation can solve pig epidemic diarrhea becomes a possibility that solves present problem.The below is the introduction of some Chinese herbal medicines.
Centella: it is cold in nature, and bitter, suffering have clearing heat and promoting diuresis, the removing toxicity for detumescence function.Mainly contain asiatic acid, madasiatic acid, asiaticosid, thankuniside, madecassoside, flavones ingredient and Asiatic polysaccharide isoreactivity material, have the raising immunity of organisms, protection intestines and stomach, anti-diarrhea effect.
Turmeric: its main active curcumin have anti-inflammatory, anti-oxidant, remove interior free yl, protection liver, improve humoral immunity of organism and cellular immunity.Modern pharmacology shows, curcumin has strong inhibitory action to the germs such as staphylococcus aureus, Escherichia coli, fungi and virus etc., can improve gut flora, control diarrhoea, curcumin can also improve T cell and the B cell of animal, thereby improves the immunocompetence of animal.
Cassia seed: be one of material of 69 kinds of integration of drinking and medicinal herbs announcing of health ministry, its nature and flavor are sweet, bitter, salty, be slightly cold, and are apt to have the effect of clearing liver and improving vision, relaxing bowel into liver, kidney, large intestine channel.
Great burdock achene: it is cold in nature, flavor is arduous, has dispelling wind and heat from the body, expelling phlegm and arresting coughing, detoxifcation promoting eruption, anti-inflammatory, the effect such as antiviral, antibiotic.
Longstamen Onion Bulb: be Chinese herbal medicine commonly used, its flavor is hot, bitter, property is wet, nontoxic, have regulate the flow of vital energy, the effect of dissipating bind.Contain multiple bioactive ingredients in Longstamen Onion Bulb, have the pharmacological actions such as anti-inflammation, platelet aggregation-against, health maintenance, reduction blood fat.
Hickory chick is under the jurisdiction of Ascomycotina (Ascomycotina), discomycete, Pezizale, Morchellaceae, morchella, and hickory chick has many small ruts with its cap surface, and the outward appearance utmost point is sheep tripe and gaining the name seemingly.It is a kind of wild famous and precious edible and medical fungi, includes the earliest in the Li Shizhen (1518-1593 A.D.) Compendium of Material Medica.The traditional Chinese medical science is used as medicine with Morchella esculenta (L.) Pers sporophore.Hickory chick is foremost delicious edible mushroom in sac fungus, and hickory chick contains protein 22.1%, fat 3.8%, polysaccharide 16.3%; And the unrighted acid in fat is 5 to 3 with the ratio of saturated fatty acid, and the useful linoleic acid of human body is accounted for 56% of fatty acid total amount.The amino acid of needed by human is complete, and its content is 5 times of Hericium erinaceus.The element that also contains the needed by human such as abundant potassium, phosphorus, calcium, iron, magnesium, zinc and selenium.Wherein the content of potassium, phosphorus is respectively 4 times and 2 times of Cordyceps sinensis, and zinc content is 9 times of Cordyceps sinensis.Selenium can change carcinogenic metabolism direction and anti-oxidant, thereby hickory chick is described as " anticancer star ".Hickory chick property is flat, distinguish the flavor of sweet cold, nontoxic, has beneficial stomach, digests the function that helps food, phlegm-eliminiating and qi-regulating, kidney tonifying, establishing-Yang, benefit brain, refreshes oneself.Research finds that hickory chick has reducing blood lipid, regulates immunity, antifatigue, anti-radiation, antitumor action, and can alleviate the toxic and side effect that cancer patient's chemicotherapy causes.
Summary of the invention:
The objective of the invention is take composite Chinese herbal powder, Chinese herbal medicine extract as main component, and the composite hickory chick microbial inoculum of science and bacillus subtilis formulation, different growth phase pig body immunity have been improved, greatly reduce antibiotic dosage, for numerous raisers provide a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea.
In order to achieve the above object, technical scheme of the present invention is as follows:
Described feed addictive is comprised of the raw material of following parts by weight:
Accumulated snow grass meal 10-15 part; Curcuma powder 5-10 part; Cassia seed powder 8-15 part; Great burdock achene powder 10-20 part; Macrostem onion powder 8-15 part; Chinese herbal and crude drugs preparations 20-35 part; Turmeric P.E 5-10 part; Hickory chick microbial inoculum 20-35 part; Bacillus subtilis microbial agent 5-10 part.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
Take centella 20-25 part; Cassia seed 10-18 part; Great burdock achene 5-15 part; Longstamen Onion Bulb 10-15 part; Respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then evenly mix and add the water of 3-6 times of weight in container, control temperature 70 C-90 ℃ maintenance 2-4h, add the mixture of 2-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 ℃-78 ℃ of maintenance 3-4h, filter; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations.
The mass ratio of described ethanol and propyl alcohol is 1:1-1.5; Concentration of alcohol is 95%; Propyl alcohol concentration is 100%;
The preparation method of described Turmeric P.E is as follows:
To be crushed to particle diameter is that below 2 millimeters, turmeric is placed in container, and the water of 3-6 times of weight of interpolation is controlled temperature 50 C-70 ℃ maintenance 2-4h, adds the mixture of 2-3 times of weight ethanol of mixed material and methyl alcohol, controls temperature to 30 ℃-40 ℃ of maintenance 3-8h, filters; Filtrate Vacuum Concentration postlyophilization obtains Turmeric P.E.
The mass ratio of described ethanol and methyl alcohol is 1:2, concentration of alcohol 85-95%.Methanol concentration 100%.
The preparation method of described hickory chick microbial inoculum is as follows:
Cultivate hickory chick from the inclined-plane switching, seed liquor after spreading cultivation is step by step transferred in fermentation tank, controlling temperature is 25-28 ℃, aerlbic culture 68-72 hour, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, ferment complete with zymotic fluid wet method super-micro wall-broken, the hyphostoma particle degree after broken wall be 0.4～0.6 micron account for 70%, then spray-drying is made the hickory chick microbial inoculum.
Described slant medium percentage by weight consists of: potato juice 20%, and glucose 2%, agar 2.0%, accumulated snow grass meal 1.2%, curcuma powder 0.8%, macrostem onion powder 1%, all the other are pure water;
The described first order seed used that spreads cultivation step by step, secondary seed, three grades of seeds, first class seed pot, secondary seed tank and fermenting and producing used medium percentage by weights compositions are: sucrose 5%, glucose 2%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, accumulated snow grass meal 2%, curcuma powder 1.2%, cassia seed powder 1.5%, great burdock achene powder 2.2%, macrostem onion powder 1.5%, all the other are pure water, pH6.0.
The preparation method of described bacillus subtilis microbial agent is as follows:
Cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and controlling temperature is 28-30 ℃, and aerlbic culture 19-24 hour, throughput was 2.0m
3/ minute; The complete centrifugation of fermenting obtains wet thallus, adds the protective agent freeze drying and obtains microbial inoculum, and protective agent consists of 10% defatted milk, 5% lactose, 5% glycerine.
Product preparation method of the present invention is as follows:
With the accumulated snow grass meal, curcuma powder, cassia seed powder, great burdock achene powder, macrostem onion powder, Chinese herbal and crude drugs preparations, Turmeric P.E, hickory chick microbial inoculum, bacillus subtilis microbial agent both mixed and got.
The addition of product of the present invention in the live pig daily ration is 500-1000 gram/ton.
Beneficial effect:
Technique scheme of the present invention has the following advantages compared to existing technology:
1. Chinese herbal medicine extract combination of the present invention contains the bioactivators such as multiple triterpene compound, glucoside, flavones, polysaccharide, has antibiotic, antiviral, enhancing immunity of organisms, promotes the effect of pig growth.Simultaneously its to have an addition few, have preventive and therapeutic action concurrently, treatment time is short, the characteristics that drug effect is more excellent.The present invention is composite by science, composite Chinese herbal powder, Chinese herbal medicine extract, hickory chick microbial inoculum and bacillus subtilis formulation etc. have been realized organic assembling, traditional Chinese medicine ingredients and bacillus subtilis formulation have particularly been added, effectively reduce the use amount of antibiotics in letting animals feed, hickory chick in incubation effectively absorption and accumulation the active component of accumulated snow grass meal, curcuma powder, cassia seed powder, great burdock achene powder and macrostem onion powder, improved the biological effect of product of the present invention.Advantage of the present invention is to adopt Chinese herbal medicine extract to add in feed, has both improved the property of medicine of Chinese herbal medicine, effectively prevent and treat various by the bacillary and viral pig epidemic diarrhea that causes, safety non-toxic again, next is less at the addition of feed, steady quality.Adopt simultaneously different Chinese herbal medicine extracts to make up, improved the effect of effect.Realize effective control pig epidemic diarrhea, improved the security of animal meat product, improved the food utilization efficiency of animal, strengthened the immunity of animal, improved raise benefit.
2. bacillus subtilis has following function: a. oxygen consumption produces acid, and the regulating intestinal canal microecological balance reaches the diseases such as prevention diarrhoea, diarrhea; B. secrete multiple enzyme, improve the feed digestive utilization ratio; C. produce various nutrients, participate in body metabolism; D. improve animal immunizing power.
3. in the present invention, the science by microorganism formulation bacillus subtilis, Chinese herbal and crude drugs preparations and natural material is composite, can effectively solve the animal immunizing power that exists in present feed cultivation low, and antibiotic dosage is too much, the problem that product quality descends.Use product of the present invention that animal immunizing power is improved a lot, antibiotic dosage reduces more than 60%, pig epidemic diarrhea has reduced 60-80%, animal product taste and meat have obvious lifting, and the organoleptic test evaluation shows that the animal product taste of use invention product and the evaluation of meat divide raising more than 65%.
The specific embodiment
Form and technique below by specific embodiment narration product of the present invention.Unless stated otherwise, in the present invention, technological means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes or change that separation and Extraction condition in these embodiments is carried out also belong to protection scope of the present invention.
The preparation of bacillus subtilis microbial agent:
(1) first order seed is cultivated: in bacillus subtilis slant strains access 500 ml shake flasks, and 100 milliliters of culture medium loading amounts, 180 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks 1000 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: with the first class seed pot of three grades of seeds take 10% inoculum concentration access total measurement (volume) as 150L, fermentation medium loading amount 100L, 28 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 24 hours;
(5) fermented and cultured: the first class seed pot bacterial classification is accessed total measurement (volume) as 1.5 tons of secondary seed tanks take 10% inoculum concentration, 1 ton of fermentation medium loading amount, 28 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 24 hours is cultivated and is finished cell concentration 5.0 * 10
8Individual/ml.
(6) centrifugation: adopt centrifuge separation and fermentation liquid to obtain thalline.
(7) vacuum freeze drying: adopt vacuum freeze-drying technique to process the acquisition dry bacteria after adding protective agent; The protective agent aqueous solution consists of defatted milk 10%, lactose 5%, glycerine 5%.Vacuum freeze-drying technique is write the culture presevation handbook referring to Institute of Microorganism, Academia Sinica, publishes in 1980. 610-653.
Fermentation medium forms: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8.
In described bacillus subtilis freeze-dried vaccine powder, number of viable is 2*10
8Individual/g.
The invention provides a kind of Feed Manufacturing bacillus subtilis (Bacillus subtilis) bacterial classification, this strain number is CGMCCNo.1.210.This bacterial strain is purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center.
The preparation of hickory chick microbial inoculum:
1, fermentation method is cultivated the process of fungal fermented filtrate:
(1) cultivation of one-level shaking flask bacterial classification: in hickory chick slant strains access first order seed shaking flask, the first order seed shaking flask is 500 milliliters of triangular flasks, 100 milliliters of culture medium loading amounts, 60 rev/mins of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours;
(2) cultivation of secondary shaking flask bacterial classification: with in 10% inoculum concentration access secondary seed shaking flask, its condition of culture is identical with first order seed with one-level shaking flask bacterial classification;
The cultivation of (3) three grades of shaking flask bacterial classifications: with secondary shaking flask bacterial classification with in three grades of seed shaking flasks of 10% inoculum concentration access, three grades of seed shaking flasks are 5000 milliliters, 1500 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours;
(4) the first class seed pot bacterial classification is cultivated: in three grades of shaking flask bacterial classification access first class seed pots, the first order seed tank volume is 50 liters, 35 liters of culture medium loading amounts, inoculum concentration 10%, 24 ℃ of cultivation temperature, 120 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 48 hours;
(5) secondary seed tank bacterial classification is cultivated: the bacterial classification that first class seed pot is cultivated accesses in the secondary seed tank, the secondary seed tank volume is 300 liters, 200 liters of culture medium loading amounts, inoculum concentration 15%, 24 ℃ of cultivation temperature, 120 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 48 hours;
(6) fermentation tank culture: in the fermentation tank that the bacterial classification access that the secondary seed tank is cultivated is 1.5 tons, 1 ton of culture medium loading amount, inoculum concentration 20%, 25 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 72 hours, fermentation ends.
2, zymotic fluid wet method super-micro wall-broken:
(1) wet method one-level broken wall: zymotic fluid is input in colloid mill with tubing pump, the gap of adjusting colloid mill stator and rotor is 1 micron, the colloid mill flow is 0.5 ton/hour, carries out mycelial one-level broken wall treatment, the granularity after the mycelium broken wall be 0.4～0.8 micron account for 70%;
(3) wet method secondary super-micro wall-broken: the adjustment homogenization pressure is 60MPa, high-pressure homogeneous flow is 0.4 ton/hour, utilizing high pressure homogenizer to carry out mycelial super-micro wall-broken processes, sampling detects through Particle Size Analyzer, sporoderm-broken rate is 100%, raw meal particle size be 40～100 nanometers account for 80%, realized the wet method super-micro wall-broken of Morchella esculenta (L.) Pers mycelium.
Hickory chick bacterium numbering of the present invention is CGMCC No.5.621, available from CGMCC.
Hyphostoma particle degree after broken wall be 0.04～0.06 micron account for 70%, then adopt spray-drying to make.
In the present invention spray-dired EAT 140-220 ℃, leaving air temp 70-80 ℃.
In the present embodiment, slant medium consists of: percentage by weight, and potato (liquor) 20%, glucose 2%, accumulated snow grass meal 1.2%, curcuma powder 0.8%, macrostem onion powder 1%, agar 2.0%, all the other are pure water.
In the present embodiment, first order seed, secondary seed, three grades of seeds, first class seed pot, secondary seed tank and fermenting and producing used mediums compositions are: percentage by weight, sucrose 5%, glucose 2%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, accumulated snow grass meal 2%, curcuma powder 1.2%, cassia seed powder 1.5%, great burdock achene powder 2.2%, macrostem onion powder 1.5%, all the other are pure water, pH6.0.
In the present embodiment during fermentation ends: pH value 4.0, the mycelia weight in wet base reaches 22%.
In the present embodiment, granularity adopts laser particle size analyzer to carry out the mensuration of granularity in zymotic fluid.
In the present invention, the process conditions of colloid mill and homogenizer are as follows: the gap of adjusting colloid mill stator and rotor is 0.5～1 micron, utilizes its shearing force effect to process, and the colloid mill flow is 0.1～1 ton/hour; The homogenizer particle size after cracking is selected 0.1～0.5 micron, utilizes the effect of the power such as its high pressure release force, void effect, shearing, carries out ultra micro and processes, and adjusting high pressure homogenizer pressure is 60～100MPa, and high-pressure homogeneous flow is 0.1～1 ton/hour;
Embodiment 1
A kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea is comprised of the raw material of following parts by weight:
10 parts of accumulated snow grass meals; 5 parts of curcuma powders; 8 parts of cassia seed powders; 10 parts, great burdock achene powder; 8 parts of macrostem onion powders; 20 parts of Chinese herbal and crude drugs preparationses; 5 parts of Turmeric P.Es; 20 parts of hickory chick microbial inoculums; 5 parts of bacillus subtilis microbial agents.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
Accurately take 20 parts of centellas; 10 parts of cassia seeds; 5 parts of great burdock achenes; 10 parts of Longstamen Onion Bulbs; Respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then evenly mix and add the water of 3 times of weight in container, control temperature 70 C and keep 2h, add the mixture of 2 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 ℃ maintenance 3h, filter; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations.
The mass ratio of described ethanol and propyl alcohol is 1:1; Concentration of alcohol is 95%; Propyl alcohol concentration is 100%;
The preparation method of described Turmeric P.E is as follows:
To be crushed to particle diameter is that below 2 millimeters, turmeric is placed in container, and the water of 3 times of weight of interpolation is controlled temperature 50 C and kept 2h, adds the mixture of 2 times of weight ethanol of mixed material and methyl alcohol, controls temperature to 30 ℃ maintenance 3h, filters; Filtrate Vacuum Concentration postlyophilization obtains Turmeric P.E.
The mass ratio of described ethanol and methyl alcohol is 1:2; Concentration of alcohol is 95%.
Product preparation method of the present invention is as follows:
With the accumulated snow grass meal; Curcuma powder; Cassia seed powder; The great burdock achene powder; Macrostem onion powder; Chinese herbal and crude drugs preparations; Turmeric P.E; The hickory chick microbial inoculum; Bacillus subtilis microbial agent mixes and get final product.
The addition of this product in the live pig daily ration is 500 gram/tons.
Embodiment 2
A kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea is comprised of the raw material of following parts by weight:
15 parts of accumulated snow grass meals; 10 parts of curcuma powders; 15 parts of cassia seed powders; 20 parts, great burdock achene powder; 15 parts of macrostem onion powders; 35 parts of Chinese herbal and crude drugs preparationses; 10 parts of Turmeric P.Es; 35 parts of hickory chick microbial inoculums; 10 parts of bacillus subtilis microbial agents.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
Accurately take 25 parts of centellas; 18 parts of cassia seeds; 15 parts of great burdock achenes; 15 parts of Longstamen Onion Bulbs; Respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then evenly mix and add the water of 6 times of weight in container, control 90 ℃ of temperature and keep 4h, add the mixture of 3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 78 ℃ maintenance 4h, filter; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations.
The mass ratio of described ethanol and propyl alcohol is 1:1.5, and concentration of alcohol is 95%; Propyl alcohol concentration is 100%;
The preparation method of described Turmeric P.E is as follows:
To be crushed to particle diameter is that below 2 millimeters, turmeric is placed in container, and the water of 6 times of weight of interpolation is controlled temperature 70 C and kept 4h, adds the mixture of 3 times of weight ethanol of mixed material and methyl alcohol, controls temperature to 40 ℃ maintenance 8h, filters; Filtrate Vacuum Concentration postlyophilization obtains.
The mass ratio of described ethanol and methyl alcohol is 1:2; Concentration of alcohol is 95%.Methanol concentration 100%.
Product preparation method of the present invention is as follows:
With the accumulated snow grass meal; Curcuma powder; Cassia seed powder; The great burdock achene powder; Macrostem onion powder; Chinese herbal and crude drugs preparations; Turmeric P.E; The hickory chick microbial inoculum; Bacillus subtilis microbial agent mixes and get final product.
The addition of this product in the live pig daily ration is 1000 gram/tons.
Embodiment 3
A kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea is comprised of the raw material of following parts by weight:
12 parts of accumulated snow grass meals; 8 parts of curcuma powders; 11 parts of cassia seed powders; 15 parts, great burdock achene powder; 11 parts of macrostem onion powders; 28 parts of Chinese herbal and crude drugs preparationses; 7 parts of Turmeric P.Es; 28 parts of hickory chick microbial inoculums; 7 parts of bacillus subtilis microbial agents.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
Accurately take 22 parts of centellas; 15 parts of cassia seeds; 10 parts of great burdock achenes; 12 parts of Longstamen Onion Bulbs; Respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then evenly mix and add the water of 5 times of weight in container, control 80 ℃ of temperature and keep 3h, add the mixture of 3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 70 ℃ maintenance 4h, filter; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations.
The mass ratio of described ethanol and propyl alcohol is 1:1.2; Concentration of alcohol is 95%; Propyl alcohol concentration is 100%.
The preparation method of described Turmeric P.E is as follows:
To be crushed to particle diameter is that below 2 millimeters, turmeric is placed in container, and the water of 5 times of weight of interpolation is controlled temperature 60 C and kept 3h, adds the mixture of 3 times of weight ethanol of mixed material and methyl alcohol, controls temperature to 35 ℃ maintenance 5h, filters; Filtrate Vacuum Concentration postlyophilization obtains.
The mass ratio of described ethanol and methyl alcohol is 1:2; Concentration of alcohol is 95%; Methanol concentration is 100%.
Product preparation method of the present invention is as follows:
With the accumulated snow grass meal; Curcuma powder; Cassia seed powder; The great burdock achene powder; Macrostem onion powder; Chinese herbal and crude drugs preparations; Turmeric P.E; The hickory chick microbial inoculum; Bacillus subtilis microbial agent mixes and get final product.
The addition of this product in the live pig daily ration is 800 gram/tons.
The result of use experiment
The result of use test of the embodiment of the present invention 1 feed addictive in weanling pig:
It is 120 of the healthy weanling pigs of (7.16 ± 0.15) kg that experimental animal is selected Yunnan subordinate plant of twins feed corporation,Ltd average weight, and statistical analysis body weight difference is not remarkable.Adopt the single-factor Randomized Designs, with 120 healthy weanling pigs, by male and female half and half, be divided into 2 groups (control group and test group), every group of 6 repetitions, each repeats 10 pigs.Test group is added the product of the present invention that embodiment 1 makes, and control group does not add product of the present invention.Fed continuously 15 days, and compared with control group, use the invention feed addictive can obtain following effect:
| Project | Control group | Test group | Deviation |
| Starting weight (kg) | 7.19 | 7.23 | -- |
| End heavy (kg) | 11.00 | 11.64 | -- |
| Daily gain (g/d ﹒ head) | 254 | 294 | 15.7% |
| Daily ingestion amount (g/d ﹒ head) | 363.2 | 367.5 | 1.2% |
[0105]?
| Feedstuff-meat ratio | 1.43 | 1.25 | -12.6% |
| Diarrhea rate (%) | 14 | 2 | -85.7% |
Result of the test shows, adds this product in the weanling pig daily ration, and the daily gain of piglet improves a lot than control group; Feedstuff-meat ratio has obvious reduction; Diarrhea rate has reduced by 85.7%.
The result of use test of the inventive example 1 feed addictive in milking sow:
Carried out the feeding experiment of 21 days by a definite date on the subordinate of twins feed corporation,Ltd pig farm, Yunnan.Single factor contrast design is adopted in test, chooses at random milking sow 30 health, that a farrowing number is close with the birth counterpoise, that parity is 2 or 3 tires, is divided at random 2 groups (being test group and control group), every group of 15 repetitions.Wherein: the test group daily ration adds this product that is prepared into by embodiment 1, and control group does not add product of the present invention.Record weaned piglet weight, diarrhea rate, the death rate.Result of the test is as follows:
| Project | Test group | Control group | Remarks |
| Produce the young number (number) of living | 10.8 | 11.0 | ? |
| Birth counterpoise (kg) | 1.46 | 1.48 | ? |
| 21 days small weaning pigs (head) | 10 | 9 | ? |
| Death rate % | 7.4 | 18.2 | ? |
| 21 days weight of weaning litters (kg) | 74.5 | 64.1 | ? |
| 21 days wean counterpoises (kg) | 7.45 | 7.12 | ? |
| Grice diarrhoea rate (%) | 3.1 | 14.3 | ? |
Result of the test shows, adds this product in lactating swine diets, and the weight ratio control group that produces porkling improves a lot; The grice diarrhoea rate has reduced by 78.3%.
Above two example tests show: product of the present invention can improve sow and piglet body immunity, significantly reduces the diarrhea rate of piglet, improves the cultivation quality and benefits.
Claims (8)
1. the Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea is comprised of the raw material of following parts by weight: accumulated snow grass meal 10-15 part; Curcuma powder 5-10 part; Cassia seed powder 8-15 part; Great burdock achene powder 10-20 part; Macrostem onion powder 8-15 part; Chinese herbal and crude drugs preparations 20-35 part; Turmeric P.E 5-10 part; Hickory chick microbial inoculum 20-35 part; Bacillus subtilis microbial agent 5-10 part; The preparation method of described Chinese herbal and crude drugs preparations is: take centella 20-25 part; Cassia seed 10-18 part; Great burdock achene 5-15 part; Longstamen Onion Bulb 10-15 part; Respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then evenly mix and add the water of 3-6 times of weight in container, control temperature 70 C-90 ℃ maintenance 2-4h, add the mixture of 2-3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 60 ℃-78 ℃ of maintenance 3-4h, filter; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations; The mass ratio of described ethanol and propyl alcohol is 1:1-1.5; The preparation method of described Turmeric P.E is as follows: will be crushed to particle diameter is that below 2 millimeters, turmeric is placed in container, add the water of 3-6 times of weight, control temperature 50 C-70 ℃ maintenance 2-4h, add the mixture of 2-3 times of weight ethanol of mixed material and methyl alcohol, control temperature to 30 ℃-40 ℃ of maintenance 3-8h, filter; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations; The mass ratio of described ethanol and methyl alcohol is 1:2.
2. a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea according to claim 1, is characterized in that, the preparation method of described hickory chick microbial inoculum is as follows:
(1) fermentation method is cultivated the process of fermented morel liquid:
(1) cultivation of one-level shaking flask bacterial classification: in hickory chick slant strains access first order seed shaking flask, the first order seed shaking flask is 500 milliliters of triangular flasks, 100 milliliters of culture medium loading amounts, 60 rev/mins of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours;
(2) cultivation of secondary shaking flask bacterial classification: with in 10% inoculum concentration access secondary seed shaking flask, its condition of culture is identical with first order seed with one-level shaking flask bacterial classification;
The cultivation of (3) three grades of shaking flask bacterial classifications: with secondary shaking flask bacterial classification with in three grades of seed shaking flasks of 10% inoculum concentration access, three grades of seed shaking flasks are 5000 milliliters, 1500 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 24 ℃ of cultivation temperature, incubation time 50 hours;
(4) the first class seed pot bacterial classification is cultivated: in three grades of shaking flask bacterial classification access first class seed pots, the first order seed tank volume is 50 liters, 35 liters of culture medium loading amounts, inoculum concentration 10%, 24 ℃ of cultivation temperature, 120 rev/mins of mixing speeds, ventilation V/V is 1:0.5, tank pressure 0.05MPa, incubation time 48 hours;
(5) secondary seed tank bacterial classification is cultivated: the bacterial classification that first class seed pot is cultivated accesses in the secondary seed tank, the secondary seed tank volume is 300 liters, 200 liters of culture medium loading amounts, inoculum concentration 15%, 24 ℃ of cultivation temperature, 120 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 48 hours;
(6) fermentation tank culture: in the fermentation tank that the bacterial classification access that the secondary seed tank is cultivated is 1.5 tons, 1 ton of culture medium loading amount, inoculum concentration 20%, 25 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 72 hours, fermentation ends;
(2) zymotic fluid wet method super-micro wall-broken:
(1) wet method one-level broken wall: zymotic fluid is input in colloid mill with tubing pump, and the gap of adjusting colloid mill stator and rotor is 0.5～1 micron, and the colloid mill flow is 0.5 ton/hour, carries out mycelial one-level broken wall treatment;
(2) wet method secondary super-micro wall-broken: the adjustment homogenization pressure is 60～100MPa, high-pressure homogeneous flow is 0.4 ton/hour, utilize high pressure homogenizer to carry out mycelial super-micro wall-broken and process, the homogenizer particle size after cracking is selected 0.1～0.5 micron, then adopts spray-drying to make the hickory chick microbial inoculum.
3. a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea according to claim 2, is characterized in that, described fermentation method is cultivated fermented morel liquid production used medium and consisted of: sucrose 5%, glucose 2%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, accumulated snow grass meal 2%, curcuma powder 1.2%, cassia seed powder 1.5%, great burdock achene powder 2.2%, macrostem onion powder 1.5%, all the other are pure water, pH6.0.
4. a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea according to claim 1, is characterized in that, in described bacillus subtilis freeze-dried vaccine powder, number of viable is 2*10
8Individual/g.
5. the described a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea of according to claim 1-4 arbitrary claims,
Raw material by following parts by weight forms: 10 parts of accumulated snow grass meals; 5 parts of curcuma powders; 8 parts of cassia seed powders; 10 parts, great burdock achene powder; 8 parts of macrostem onion powders; 20 parts of Chinese herbal and crude drugs preparationses; 5 parts of Turmeric P.Es; 20 parts of hickory chick microbial inoculums; 5 parts of bacillus subtilis microbial agents.
6. the described a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea of according to claim 1-4 arbitrary claims,
Raw material by following parts by weight forms: 15 parts of accumulated snow grass meals; 10 parts of curcuma powders; 15 parts of cassia seed powders; 20 parts, great burdock achene powder; 15 parts of macrostem onion powders; 35 parts of Chinese herbal and crude drugs preparationses; 10 parts of Turmeric P.Es; 35 parts of hickory chick microbial inoculums; 10 parts of bacillus subtilis microbial agents.
7. the described a kind of Chinese herbal medicine extract feed addictive that can effectively prevent and treat pig epidemic diarrhea of according to claim 1-4 arbitrary claims,
Raw material by following parts by weight forms: 12 parts of accumulated snow grass meals; 8 parts of curcuma powders; 11 parts of cassia seed powders; 15 parts, great burdock achene powder; 11 parts of macrostem onion powders; 28 parts of Chinese herbal and crude drugs preparationses; 7 parts of Turmeric P.Es; 28 parts of hickory chick microbial inoculums; 7 parts of bacillus subtilis microbial agents.
8. according to claim 1-7 arbitrary described a kind of preparation methods that can effectively prevent and treat the Chinese herbal medicine extract feed addictive of pig epidemic diarrhea, comprise the steps:
With the accumulated snow grass meal; Curcuma powder; Cassia seed powder; The great burdock achene powder; Macrostem onion powder; Chinese herbal and crude drugs preparations; Turmeric P.E; The hickory chick microbial inoculum; Bacillus subtilis microbial agent both mixed and got;
The preparation method of described hickory chick microbial inoculum is as follows:
Cultivate hickory chick from the inclined-plane switching, seed liquor after spreading cultivation is step by step transferred in fermentation tank, controlling temperature is 25-28 ℃, aerlbic culture 68-72 hour, 100 rev/mins of mixing speeds, ventilation V/V is 1:0.5, tank pressure 0.05MPa, the complete zymotic fluid wet method super-micro wall-broken that ferments, the hyphostoma particle degree after broken wall be 0.4～0.6 micron account for 70%, then spray-drying is made the hickory chick microbial inoculum;
Described first order seed, secondary seed, three grades of seeds, first class seed pot, secondary seed tank and fermenting and producing used mediums compositions are: sucrose 5%, glucose 2%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, accumulated snow grass meal 2%, curcuma powder 1.2%, cassia seed powder 1.5%, great burdock achene powder 2.2%, macrostem onion powder 1.5% complements to 100% pure water, pH6.0;
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
Accurately take 25 parts of centellas; 18 parts of cassia seeds; 15 parts of great burdock achenes; 15 parts of Longstamen Onion Bulbs; Respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then evenly mix and add the water of 6 times of weight in container, control 90 ℃ of temperature and keep 4h, add the mixture of 3 times of weight ethanol of mixed material and propyl alcohol, control temperature to 78 ℃ maintenance 4h, filter; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations;
The mass ratio of described ethanol and propyl alcohol is 1:1.5;
The preparation method of described Turmeric P.E is as follows:
To be crushed to particle diameter is that below 2 millimeters, turmeric is placed in container, and the water of 6 times of weight of interpolation is controlled temperature 70 C and kept 4h, adds the mixture of 3 times of weight ethanol of mixed material and methyl alcohol, controls temperature to 40 ℃ maintenance 8h, filters; Filtrate Vacuum Concentration postlyophilization obtains; The mass ratio of described ethanol and methyl alcohol is 1:2.
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| CN103931922A (en) * | 2014-03-24 | 2014-07-23 | 安徽宏亮饲料科技有限公司 | Pig feed by taking wheat and cassava as main materials |
| CN104686797A (en) * | 2014-11-21 | 2015-06-10 | 天津天绿健科技有限公司 | Method for preparing probiotic microcapsules for feed |
| CN105614113A (en) * | 2016-03-10 | 2016-06-01 | 徐杰 | Piglet anti-diarrhea compound Chinese herbal medicine feed and preparing method thereof |
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| CN103689276A (en) * | 2013-12-16 | 2014-04-02 | 双胞胎（集团）股份有限公司 | Chinese herbal medicine feed additive capable of effectively preventing and treating piglet diarrhea |
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| CN107348193A (en) * | 2017-07-28 | 2017-11-17 | 烟台市昊星晟信息技术有限公司 | The preparation method of one boar food |
| CN114271389A (en) * | 2021-12-23 | 2022-04-05 | 广东加大实业有限公司 | Weaned piglet feed and preparation method thereof |
| CN114271390A (en) * | 2021-12-23 | 2022-04-05 | 广东加大实业有限公司 | Plant feed for piglets and preparation method thereof |
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Effective date of registration: 20210326 Address after: 541300 Guixing village, industrial park, Xing'an County, Guilin City, Guangxi Zhuang Autonomous Region Patentee after: Xing'an twin Feed Co.,Ltd. Address before: Alacun, alaxiang, Kunming Economic and Technological Development Zone, Yunnan Province (No. 711, huangkun Road) Patentee before: YUNNAN TWINS FEED Co.,Ltd. |