CN103141513B - Aqueous agent containing lentinan and ammonium molybdate, and preparation method thereof - Google Patents
Aqueous agent containing lentinan and ammonium molybdate, and preparation method thereof Download PDFInfo
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- CN103141513B CN103141513B CN201310053991.5A CN201310053991A CN103141513B CN 103141513 B CN103141513 B CN 103141513B CN 201310053991 A CN201310053991 A CN 201310053991A CN 103141513 B CN103141513 B CN 103141513B
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- ammonium molybdate
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Abstract
The invention belongs to the technical field of biogenic pesticides, and concretely relates to an aqueous agent containing lentinan and ammonium molybdate, and a preparation method thereof. The effective components of the aqueous agent comprise 0.45-0.55% by mass of lentinan and 0.9-1.1% by mass of ammonium molybdate. The preparation method comprises the following steps: taking a lentinan mother solution, and adding water to prepare an ingredient 1; 2, taking ammonium molybdate, adding water, and adding glycine in a dropwise manner to make the pH value of the obtained solution not greater than 7 in order to obtain an ingredient 2; 3, mixing the ingredient 1 with the ingredient 2 in proportion until the lentinan mass percentage reaches 0.45-0.55% and the ammonium molybdate mass percentage reaches 0.9-1.1%, and adding water to a certain amount; and 4, emulsifying, filtering, examining, and packaging to obtain the aqueous agent containing lentinan and ammonium molybdate. The aqueous agent containing lentinan and ammonium molybdate has the advantages of long persistent period, no disease condition rebounding, small application amount, good absorption, fast effectiveness, rain washing resistance, wide spectrum, safety, high efficiency, and improvement of the quality characteristics of plants, and can be used for treating, preventing and killing tobacco virus diseases, cucurbits virus diseases and the like.
Description
Technical field
The invention belongs to biogenic pesticide technical field, be specifically related to a kind of lentinan molybdenum aqua and preparation method thereof.
Background technology
The viroses of plant are by the parasitogenic disease of plant virus.The plant that is injured often shows following symptom: 1. variable color.Because nutriment is utilized by virus, or virus causes vascular bundle necrosis to hinder the transport of nutriment, and the chlorophyll formation of blade is obstructed or gathers, thus produces floral leaf, spot, ring spot, arteries and veins band and yellow etc.Thus the anthocyan of flower also can change, and make pattern become green or variegated etc., common symptom is that dark green and light green alternate floral leaf disease is as tobacco mosaic.2. downright bad.Because the allergic reaction etc. of plant to virus can cause cell or tissue dead, become withered and yellow to brown, sometimes occur depression.Blade often presents necrotic plaque, downright bad ring and arteries and veins downright bad, often occur downright bad bar etc. on the surface of stem, fruit and root.3. deformity.Because the normal metabolism of plant is disturbed, the generation of tumor growth element and other hormones and plant grow normally and change, metallaxis can be caused, as shortened between stem, plant is downgraded, growing point abnormal differentiation forms clump branch or clump bunch, and blister spot, curling, fern leaf and bandization etc. appear in the local cells distortion of blade.
On existing market, widely use lentinan (being mushroom proteoglycan again) and prevent and treat the viroses of plant, though common lentinan is cured the disease, poison is effective, but effect is obvious not, even rebounds within the week.
Summary of the invention
When the object of the present invention is to provide one to prevent and treat the viroses of plant, the state of an illness does not rebound, consumption is little, the lentinan molybdenum aqua of instant effect and preparation method thereof.
For achieving the above object, the technical scheme taked of the present invention is as follows:
A kind of lentinan molybdenum aqua, by mass percentage, its active ingredient is: lentinan 0.5 ± 0.05%, ammonium molybdate 1.0 ± 0.1%.
The effect of active ingredient: lentinan (being mushroom proteoglycan again): 1, to the virus disease of plant, there is treatment and prevention effect; 2, have and kill and wound object and infectivity germ cell, the effect of protection host cell; 3, metabolic factor stimulating plant, increases immunologic function; 4, the protease in lentinan is combined with molybdenum ion, stimulates and repair cell, promotes that primary juice flows, having positive effect to crop root lignification, fruit chorionitis, fruit-shrink disease, early ageing disease; 5, the hydrogen on the hydroxyl in polysaccharide is replaced by molybdenum, forms organic-molybdenum, to crop safety.Molybdenum: 1, itself be the indispensable important element of primary juice; 2, as carrier, promote that primary juice flows; 3, molybdenum is the part of plant polyphenol enzyme, can stimulate and activate vascular bundle cell, prevent vascular bundle meronecrosis or lignification.
A preparation method for lentinan molybdenum aqua, step is as follows:
(1), get mushroom polysaccharide mother liquor, add water, obtain batching 1;
(2), get ammonium molybdate, add water, drip glycine, make pH≤7 of solution, obtain batching 2;
(3), by batching 1 and batching 2 to mix in proportion, make the mass percentage of lentinan reach 0.5 ± 0.05%, the mass percentage of ammonium molybdate reaches till 1.0 ± 0.1%, surplus is water;
(4), namely this product is obtained through ultra-fine emulsification, filtration, test package.
Prove through indoor measurement, it is long that lentinan molybdenum aqua of the present invention has the lasting period, and the state of an illness does not rebound, and consumption is little, good absorbing, instant effect, resistance to rain washes away, wide spectrum, safety, efficiently, improves the characteristic of plant quality, can be used for treating, preventing and root out tobacco virus, melon virus disease etc.Lentinan molybdenum aqua of the present invention has the effect increasing chlorophyll, improve output, improve quality simultaneously.Through field control effectiveness test and Demonstration And Extension, achieve the positive effect of disease-preventing and yield-increasing, there is good application value.
Embodiment
For the lentinan molybdenum aqua of 100kg, by mass percentage, its active ingredient is: lentinan 0.5 ± 0.05%, ammonium molybdate 1.0 ± 0.1%.
Be prepared as follows, following percentage composition is mass percentage:
(1), in order to reduce overlapping investment, will commercially buy 2% qualified mushroom polysaccharide mother liquor 25kg, and adding the water of 25kg, be placed in material-compound tank and must prepare burden 1;
(2), the ammonium molybdate of getting 1kg puts into another material-compound tank, drips glycine again, make pH≤7 of solution, obtain batching 2 after adding the water (reaching the content of 2%) of 50kg;
(3), in final material-compound tank, by batching 1 and batching 2 mixing, the mass percentage of lentinan reaches 0.5%, the mass percentage of ammonium molybdate reaches 1.0%, and all the other are water;
(4), through ultra-fine emulsification, filtration, test package namely obtain product, this product appearance is pale yellow transparent aqueous emulsion.
one, tobacco virus test is prevented and treated
1 test objective
Verify that the present invention 0.5% lentinan molybdenum aqua is to the control efficiency of tobacco virus, for reagent agent provides field best using dosage.
2 experimental conditions
The selection of 2.1 subjects, crop and kind
Tobacco virus (TMV);
Tobacco bred is NC89.
2.2 environmental condition
This test arrangement is the solid little Shi Cun in Xiangxiang in Linying County, tobacco planting area 2 mu, this field land readjustment, and water and fertilizer condition is good, and managerial skills are medium, and tobacco is in group's phase, and virus disease starts, and soil property is two conjunction soil, pH value 6.9.Each experimental plot soil, growing way, cultivation and fertilizer and water condition are basically identical, and conform to local agricultural production is actual.
3 experimental scheme and arrangement
3.1 medicament
3.1.1 test medicine
0.5% lentinan molybdenum aqua (preparation of specific embodiment of the invention part).
3.1.2 medicament is contrasted
0.5% lentinan aqua (Beijing YanHuaYongLe pesticide Co., Ltd produces, commercially available product).
3.1.3 dosing is numbered with process
3.2 communities arrange
3.2.1 community arrangement
3.2.2 plot area and repetition
Plot area: 50 square metres;
Number of repetition: repeat for 4 times.
3.3 application method
3.3.1 using method
After test arrangement dosage dispensing, with manual sprayer, even spraying is carried out to the whole strain of tobacco.
3.3.2 spraying time and number of times
On June 20th, 2007 carries out dispenser, and on June 27th, 2007 carries out the 2nd dispenser, and tobacco is in group's phase, and virus disease starts life.
3.4 use capacity
Actual hectare amount of liquid medicine 750 liters.
4 investigation, record and measuring method
4.1 meteorological and soil informations
4.1.1 meteorological data
Become a fine day during first time dispenser 4 dispensers in afternoon on June 20th, 2007, gentle breeze, average daily temperature 27.4 DEG C, relative moisture 64%; Mean wind speed 1.2 meter per second, becomes a fine day during second time dispenser 4 dispensers in afternoon on June 27, temperature 27.4 DEG C, relative moisture 65%; Mean wind speed 1.5 meter per second, concrete meteorological data (referring to table 1-3,1-4).
4.1.2 soil information
Experimental field is two conjunction soil, and pH value 6.9, water and fertilizer condition is good, and soil is more smooth.
4.2 investigation methods, time and number of times
4.2.1 control time and number of times
Investigation state of an illness radix before dispenser on June 20 in 2007, after second time dispenser, 7 days investigation morbidity strain numbers and control efficiency, investigate 2 times altogether.
4.2.2 investigation method
Random searching 50 strain of every community, record virus disease morbidity progression, listing mark.
Grade scale:
0 grade: without any symptom;
1 grade: the bright arteries and veins of leaf or light floral leaf;
3 grades: lobus cardiacus and middle leaf sheet floral leaf, there is necrotic plaque in leaf sometimes;
5 grades: most blade floral leaf, minority leaf malformation, shrinkage, there is necrotic plaque in blade or stem sometimes, or billet spot appears in stem;
7 grades: most leaf malformation, elongated, or cane, vein produce, and system is downright bad, plant is downgraded;
9 grades: plant serious systemic floral leaf, deformity, or serious systemic is downright bad sometimes, and plant is obviously downgraded, even dead.
4.2.3 drug effect computational methods
According to field investigation result, calculate disease index and control efficiency.
Total strain number × 9 of disease index=∑ (the sick number of sheets × relative level numerical value at different levels) × 100/ investigation
Control efficiency (%)=(1-CK
0× PT
1/ CK
1× PT
0) × 100
Note: CK
0: disease index before blank dispenser;
CK
i: disease index after blank dispenser;
PT
0: disease index before the dispenser of chemicals treatment district;
PT
1: disease index after the dispenser of chemicals treatment district;
4.3 direct impacts on crop
According to test dose medication, do not find there is any impact to tobacco growing, do not have poisoning phenomenon to occur.
4.4 affect other biological
4.4.1 on the impact of other damage by disease and insect
Have no and have impact to other damage by disease and insect of tobacco.
4.4.2 on the impact of other non-target organisms
Do not find to affect the beneficial organism such as fish and frog.
5 results and analysis
0.5% 15 grams/ha, 13.125 grams/ha, 11.25 gram/ha 3, lentinan molybdenum aqua process is respectively 81.3%, 77.8%, 72.9% to the control efficiency of tobacco virus, and between preventive effect, otherness is not remarkable.Contrast medicament 0.5% lentinan aqua 13.125 grams/ha of control efficiency to tobacco virus are 64.2%; By 0.5% lentinan molybdenum aqua control tobacco virus control efficiency, than 0.5% lentinan aqua, there were significant differences, to tobacco safety.
Suggestion, at tobacco virus their early stage, adopts 11.25 ~ 15 grams/ha of sprayings of 0.5% lentinan molybdenum aqua.
two, the test of cucumber virus disease is prevented and treated
1 test objective
By testing clear and definite the present invention 0.5% lentinan molybdenum aqua to the control efficiency of cucumber virus disease, best using dosage and the safety to cucumber thereof.
2 experimental conditions
The selection of 2.1 subjects, crop and kind
Cucumber virus disease;
Trial crops: cucumber (kind is No. 8, yellow agriculture).
2.2 environmental condition
Experimental field be selected in watermelon production field, Xi Geng village of Zhao Gu township of Huixian City of Henan Province, area 0.30 hectare, get intermediary massif and to do experiment field.The same land for growing field crops of other control measures, experimental field.
3 experimental scheme and arrangement
3.1 medicament
3.1.1 test medicine
0.5% lentinan molybdenum aqua (preparation of specific embodiment of the invention part).
3.1.2 medicament is contrasted
0.5% lentinan aqua (Beijing YanHuaYongLe pesticide Co., Ltd produces, commercially available product).
3.1.3 dosing is numbered with process
3.2 communities arrange
3.2.1 community arrangement
3.2.2 plot area and repetition
Plot area: 33m
2(6m × 5.5m);
Number of repetition: repeat for four times.
3.3 application method
3.3.1 using method
When cucumber puts climing rear beginning result, whole strain spraying after being diluted to design dosage.
3.3.2 spraying equipment
Terre verte ground-3WBS-16 type manual sprayer.
3.3.3 spraying time and number of times
When field finds diseased plant for the first time, (on May 15th, 2008) spraying once.
3.3.4 capacity is used
By designing requirement, be watered 750 liters/hectare.
4 investigation, record and measuring method
4.1 meteorological and soil information
4.1.1 meteorological data
4.1.2 soil information
Soil is husky conjunction soil, and fertility is better-than-average, the content of organic matter 1.4%, pH value 7.0.
4.2 investigation methods, time and number of times
4.2.1 control time and number of times
Investigation radix before medicine, 14 days investigation diseased plant numbers after dispenser.
4.2.2 investigation method
All in investigation community have diseased plant number, records virus disease morbidity strain number and investigate total strain number.
4.2.3 drug effect computational methods
According to " pesticide field efficacy medicine test criterion ", drug effect computational methods are as follows:
4.3 direct impacts on crop
Investigation in 14 days after medicine, uses 0.5% lentinan molybdenum aqua control cucumber virus disease, has no adverse effects to cucumber normal growth.
5 results and analysis
5.1 result of the test
15 grams/ha, test medicine 0.5% lentinan molybdenum aqua process preventive effect is up to 74.17%; Test medicine 13.125 grams/ha of process are taken second place, and average preventive effect is 65.00%; The average preventive effect of 13.125 grams/ha, contrast medicament 0.5% lentinan aqua is 62.08%; The average preventive effect of test medicine 11.25 grams/ha process is minimum, is only 56.67%.By test, can effectively prevent and treat cucumber virus disease with 0.5% lentinan molybdenum aqua.
In sum, pat equal control efficiency everywhere see according to test, by 0.5% lentinan molybdenum aqua control cucumber virus disease in production, large scale application recommended dose is with 13.125-15 gram/ha of better effects if.
6 conclusions
6.1 can prevent and treat cucumber virus disease effectively with 0.5% lentinan molybdenum aqua, have no adverse effects to cucumber growth.
6.2, by 0.5% lentinan molybdenum aqua control cucumber virus disease, apply recommended dose with 13.125-15 gram/ha of better effects if.
Claims (2)
1. a lentinan molybdenum aqua, is characterized in that obtaining by the following method:
(1), get mushroom polysaccharide mother liquor, add water, obtain batching 1;
(2), get ammonium molybdate, add water, drip glycine, make pH≤7 of solution, obtain batching 2;
(3), by batching 1 and batching 2 to mix in proportion, make the mass percentage of lentinan reach 0.5 ± 0.05%, the mass percentage of ammonium molybdate reaches till 1.0 ± 0.1%, surplus is water;
(4), namely this product is obtained through emulsification, filtration, test package.
2. the preparation method of lentinan molybdenum aqua as claimed in claim 1, is characterized in that step is as follows: (1), get mushroom polysaccharide mother liquor, adds water, obtains batching 1;
(2), get ammonium molybdate, add water, drip glycine, make pH≤7 of solution, obtain batching 2;
(3), by batching 1 and batching 2 to mix in proportion, make the mass percentage of lentinan reach 0.5 ± 0.05%, the mass percentage of ammonium molybdate reaches till 1.0 ± 0.1%, surplus is water;
(4), namely this product is obtained through emulsification, filtration, test package.
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|---|---|---|---|
| CN201310053991.5A CN103141513B (en) | 2013-02-20 | 2013-02-20 | Aqueous agent containing lentinan and ammonium molybdate, and preparation method thereof |
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| CN103141513B true CN103141513B (en) | 2015-02-18 |
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| CN103960296A (en) * | 2014-04-24 | 2014-08-06 | 山东圣鹏科技股份有限公司 | Plant virus disease resisting composition and application thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8920011A (en) * | 1988-01-12 | 1990-10-01 | Japan Tobacco Inc | CHEMICALS FOR PLANT PROTECTION AND PLANT VIRUS REMOVAL, AND MANUFACTURE FOR A METHOD THEREOF. |
| CN1027785C (en) * | 1993-03-20 | 1995-03-08 | 辽宁省出口食用菌研究所 | Agricultural chemical prepared from edible fungus for curing and preventing plant virus |
| CN1087903C (en) * | 1996-01-19 | 2002-07-24 | 济南科贝尔生物工程有限公司 | Agricultural chemical for preventing and controlling plant virus prepared by liquid fermentation and its producing technology |
| CN1124784C (en) * | 2000-04-20 | 2003-10-22 | 王先彬 | Biological Pesticide prepared with the extraction from xianggu mushroom leftover and the preparation process |
| CN101297648B (en) * | 2007-11-22 | 2010-12-29 | 北京燕化永乐农药有限公司 | Bactericide agent composition containing mushroom proteoglycan |
| CN102172247A (en) * | 2011-03-03 | 2011-09-07 | 广西田园生化股份有限公司 | Composition for preventing and treating virus diseases of crops |
| CN102765987B (en) * | 2012-07-05 | 2014-09-24 | 永仁远南农业综合开发有限公司 | Multi-resistant nutrient for plant growth and preparation method thereof |
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