CN103099865A - Traditional Chinese medicine composition and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition, and belongs to the field of Chinese herbal preparation. The traditional Chinese medicine composition is prepared by mixing 6-12 parts of cortex mori radicis, 3-6 parts of fenugreek seeds and 3-6 parts of coptis root, and carrying out water extraction, alcohol precipitation, ammonia water elution, concentration and drying, wherein the cortex mori radicis has remarkable functions of inhibiting alpha-glucosaccharase, delaying the absorption rate of glucose and fructose and reducing the postprandial blood glucose concentration after meal; the fenugreek seeds can obviously reduce the blood glucose level of a glycuresis mice, has the functions of adjusting the lipid metabolism, reducing triglyceride and cholesterol and rising high-density lipoprotein; and the natural bases extracted from the coptis root, particularly berberine, can activate adenine mononucleotide protein kinase (AMPK) in cells, enhance the consumption of glucose by cells, reduce the insulin resistance, etc. The extracts of the three traditional Chinese medicine can effectively reduce the blood glucose level, has a more remarkable effect in comparison with the traditional Chinese medicine with a single component, and can be used for preparing the traditional Chinese medicine composition for preventing and treating glycuresis.
Description
Technical field
The invention belongs to the Chinese medicine preparation technical field, be specifically related to a kind of Chinese medicine composition that contains Cortex Mori, Semen Trigonellae, Rhizoma Coptidis and preparation method and purposes with blood sugar reducing function.
Background technology
Diabetes are chronic, general, metabolic diseases that a kind of inherited genetic factors and the long-term combined effect of environmental factors cause, increasing as feature take plasma glucose levels, is mainly to affect a kind of disease of normal physiological activity because of hypoinsulinism in body or effect the obstacle sugar, fat, the protein metabolism disorder that cause.
If diabetes obstinate, the state of an illness are controlled, acute and chronic complication not occuring with great difficulty, involves each organ of whole body, tissue, very harmful.The Diabetic Acute complication has hypoglycemia, diabetic ketoacidosis, hyperosmolar nonketotic diabetic coma, diabetes lactic acidosis.Chronic complicating diseases of diabetes is mainly trunk and microangiopathies, wherein little, circulatory disturbance of cardiovascular disease, nephropathy, diabetic foot, diabetic ophthalmopathy, multi-infection etc. is the common complication of diabetes, and the life security of diabetics in serious threat.Relevant survey data shows; In diabetics, the probability of concurrent cardiovascular and cerebrovascular disease is 26%, and the probability of peripheral neuropathy is 17.8%, and ocular disease is 14.8%, and the probability of nephropathy is 10.6%.
Diabetes are at present still without the radical cure medicine, the orally-taken blood sugar reducing medicine of clinical treatment type ii diabetes used can be divided into medicine (sulfonylurea drugs, glinides), the medicine (biguanides, Thiazolidinediones) that promotes insulin action that promotes the insulin secretion agent and the medicine (a-glycosidase inhibitor) that reduces the interior infiltration rate of glucose intestinal according to the difference of mechanism of action.In a few class oral antidiabetic drug, the blood sugar reducing function of sulfonylurea drugs is the strongest, is recommended as by the diabetes guide of a plurality of countries and international organization's formulation the main medication of controlling type ii diabetes ward mate hyperglycemia at present.Sulfonylurea drugs in China's listing is mainly glibenclamide, glimepiride, glipizide, gliquidone and gliclazide at present.The sulfonylurea drugs main pharmacological is to stimulate the beta Cell of islet excreting insulin, increases the insulin level in body.The modal untoward reaction of sulfonylurea drugs is hypoglycemic reaction, can also cause body weight to increase, and also has other rare untoward reaction as nauseating, vomiting, abnormal liver function, leukopenia, anemia, thrombocytopenia, erythra etc.
The hypoglycemic effect of chemicals is obvious, but long-term taking is prone to untoward reaction, brings out multiple complications, the situation of drug failure sometimes also can occur, the sulfonylurea drugs secondary failure that mostly occurs.Multinomial studies show that in recent years, Chinese medicine has positive effect in the appearance of controlling blood glucose and control diabetic complication and aspect increasing the weight of.Diabetes, the traditional Chinese medical science is referred to as " quenching one's thirst ", the meaning of the excessive thirst of namely becoming thin, major lesions position be at lung, stomach, kidney, now the mechanism of TCM Treatment of Diabetes, the one, by the comprehensive adjustment effect, tonifying five ZANG-organs, beneficial vital essence, the tired blood of dispelling, treating both the principal and the secondary aspects of a disease at the same time makes imbalance of YIN and YANG, QI and blood disorder, the weak recovery of function in body normal; The 2nd, Chinese medicine has clear and definite blood sugar reducing function.China has abundant natural resources of Chinese medicinal materials and application experience, and the Chinese medicine that has been proved blood sugar reducing function at present reaches more than 70 and plants.The mechanism of Chinese medicine blood sugar lowering may have the following aspects: promote the beta Cell of islet excreting insulin, suppress the secretion of glicentin; Improve combination rate of insulin receptor and number, improve the Insulin receptor INSR after effect; Suppress glyconeogenesis, promote glucose utilization, delay the absorption of intestinal glucose.China's herb resource is abundant, starts with from digestion, absorption, the metabolic pathway of sugar and protein, with modern medical theory explanation traditional Chinese medical science mechanism, develops safe and reliablely, and effective ingredient is clear and definite, and action target natural hypoglycemic medicine clearly provides a new approach.
Summary of the invention
For the deficiencies in the prior art, the present invention is based on the principle of modern pathology molecular mechanism and Traditional Chinese medical theory combination, aim to provide a kind of Chinese medicine composition for prevention and treatment diabetes.This Chinese medicine composition is comprised of Cortex Mori, Semen Trigonellae, three kinds of Chinese medicines of Rhizoma Coptidis, utilizes the ingredient combination of three kinds of different blood sugar lowering mechanism, plays collaborative hypoglycemic activity, and blood sugar reducing function is obvious.
For achieving the above object, technical scheme of the present invention is:
A kind of Chinese medicine composition, it is after being mixed by the raw material of following weight portion, to get extracting solution through water extraction; Extracting solution is the lime cream of 5%-50% with adding mass concentration, after shaking up, then adds the ethanol precipitation, and the quality final concentration that makes ethanol is 40%-80%, and supernatant is got in centrifugalize; Supernatant by cationic exchange resin adsorption, is ammonia eluting, the collection ammonia eluent of 0.20%-5.0% with mass concentration, the extract that obtains after concentrate drying:
Cortex Mori 6-12 part;
Semen Trigonellae seed 3-6 part;
Rhizoma Coptidis 3-6 part.
The weight portion composition of described raw material is preferably:
Cortex Mori 6-9 part;
Semen Trigonellae seed 3-4.5 part;
Rhizoma Coptidis 3-4.5 part.
The weight portion of described raw material forms more preferably:
6 parts of Cortex Mori;
3 parts, Semen Trigonellae seed;
3 parts of Rhizoma Coptidis.
Described Rhizoma Coptidis is preferably Rhizoma Coptidis.
The dosage form of described Chinese medicine composition comprises powder, granule, tablet or capsule.
Another technical problem that will solve of the present invention is to extract the active component with blood sugar lowering from natural plants Cortex Mori, Semen Trigonellae seed, Rhizoma Coptidis, makes Chinese medicine composition.
For achieving the above object, technical scheme two of the present invention is: the preparation method of described Chinese medicine composition, and concrete steps are:
(1) according to above-mentioned weight portion proportioning, with Cortex Mori, Semen Trigonellae seed and Rhizoma Coptidis mix homogeneously, get raw mix; Add the water with respect to raw mix 8-10 times weight, soaked 20-30 minute, then reflux 20-30 minute, filter, keep filtrate a; Add again the water of 8-10 times of weight in filtering residue, soaked 20-30 minute, then reflux 20-30 minute, filtrate b kept; Merging filtrate a and filtrate b get the filtrate mixture; It is 1.02-1.20 that the filtrate mixture is concentrated into relative density;
(2) first adding mass concentration in the filtrate mixture after concentrated is the lime cream of 5%-50%, shakes up, then adds the ethanol precipitation, and the quality final concentration that makes ethanol is 40%-80%, and supernatant is got in centrifugalize;
(3) supernatant is regulated pH to 2.0-5.0, pass through cationic exchange resin adsorption except precipitation is rear, after the washing remove impurity, be 0.20%-5.0% ammonia eluting resin with mass concentration, collect the ammonia eluent, concentrate drying namely gets Chinese medicine composition of the present invention.
Add the quality of entry in step (1), be preferably for the first time the water with respect to 10 times of weight of raw mix, be preferably for the second time the water with respect to 8 times of weight of raw mix.
Filtrate mixture described in step (1) concentrated preferably to relative density be 1.05-1.15.
Described in step (2), the mass concentration of lime cream is preferably 15%-30%; Described in step (2), the quality final concentration of ethanol is preferably 50%-60%.
In step (3), the preferred sulfur acid for adjusting pH of using is to 3.0-4.0.
Cation exchange resin described in step (3) is preferably one or more in D001 type cation exchange resin, 001 * 4 type gel type resin, 001 * 7 type gel type resin and 001 * 1II type gel type resin.
Described in step (4), the mass concentration of ammonia is preferably 1.5%-2.5%.
The present invention also provides the application of described Chinese medicine composition in preparation prevention and treatment diabetes medicament.
Can take according to the dose size of doctor's advice by blood-sugar lowering tcm drug compositions, diabetics that above-mentioned preparation method obtains.Generally speaking, take the capsule of every 0.4g as example, adult every day three times, each two to three, warm boiled water, two months is a course for the treatment of.
The below does further explanation and explanation to the present invention:
The invention provides a kind of Chinese medicine composition with the combination of different blood sugar lowering mechanism, said composition is comprised of Cortex Mori, Semen Trigonellae, three kinds of Chinese medicines of Rhizoma Coptidis, Cortex Mori has the effect of significant inhibition a-glucosidase, the infiltration rate of delay glucose and fructose, thus reduce post-prandial glycemia; The Semen Trigonellae seed can obviously reduce the blood sugar level of diabetic mice, also can play and regulate the lipid metabolism effect, and triglyceride, cholesterol reduction, high density lipoprotein are raise; Several alkaloids that extract in Rhizoma Coptidis, main berberine can active cell in AMPK vigor, increase cell to the consumption of glucose, reduce the effect such as insulin resistant.By the combination of these three kinds of Chinese medicine extract, can effectively reduce blood sugar level, with respect to the Chinese medicine of each single component, its more remarkable effect can be for the preparation of the Chinese medicine composition of preventing and treating diabetes.
This Chinese medicine composition selects several Chinese medicine materials to make extract (Cortex Mori extract, Semen Trigonellae seed extract, Rhizoma Coptidis extract), from different mechanism start with the treatment diabetes, reach combination of long drives and drop shots, the effect for the treatment of both the principal and secondary aspects of a disease all breaks through and improves from efficacy and saferry.
Cortex Mori (Cortex Mori) is the dry root bark of Moraceae Morus plant Morus alba L., and property is sweet, cold, returns lung meridian, has eliminating pathogen from the lung for relieving asthma, inducing diuresis to remove edema effect, cures mainly dyspnea and cough due to lung-heat, edema distension oliguria, appearance skin edema.Compendium of Materia Medica " record of " Cortex Mori cures mainly the frequent micturition of quenching one's thirst " arranged.Japanese scholars has carried out suppressing the active testing of glycosidase to 266 kinds of crude drugs, find that Cortex Mori has higher inhibition active to this enzyme, the diabetes rat of isolated active substance through being accredited as 1-deoxynojirimycin STZ being induced has remarkable blood sugar reducing function from the hot water extract of Cortex Mori.Research thinks that its blood sugar lowering approach may be the inhibitory action of white mulberry rootbark general flavone to disaccharidase activity on the small intestinal [Dan, slows down small intestinal to digestion and the absorption of carbohydrate, and the periphery change of blood sugar is tended towards stability.
Semen Trigonellae is the dry mature seed of leguminous plant Semen Trigonellae Trigonellafoenum-graecum L..As Chinese medicine, return kidney channel, bitter in the mouth warm in nature; The kidney warming, cold relieving, pain relieving; Be used for the cold void of kidney, cold and pain in the lower abdomen, inguinal hernia, the disturbance of lower legs due to pathogenic cold and dampness.The active ingredients such as fenugreek seed rich cellulose class, amino acids (especially 4-base isoleucine), alkaloids, saponins.The fenugreek seed extract is it to be mainly contained the enrichment method thing of effective constituent.In extract product of fenugreek seeds, cellulose can reduce degree of absorption to fat at enteral, and the absorption of sugar is slowed down, and accelerates glucose metabolism, increases Insulin receptor INSR sensitivity on every side, thereby controls, reduces blood sugar level.Cellulose can make on type i diabetes patient mononuclear cell Insulin receptor INSR in conjunction with increase, thereby saves the requirement of insulin.Amino acids in the fenugreek seed extract is 4-hydroxyisoleucine especially, can stimulate the secretion of insulin, directly helps to control, reduce blood sugar level.The cellulose family composition also helps to reduce low-density albumen and the total cholesterol level (blood fat reducing function) in blood, improves blood circulation.The cellulose family composition also helps to improve gastrointestinal movement, anti-treating constipation, weight-reducing.
Rhizoma Coptidis bitter in the mouth, cold in nature enters the heart, liver, stomach, large intestine channel, but heat clearing and damp drying, and eliminating fire and detoxication is as the common drug of Chinese traditional treatment diabetes and be widely used by clinical always.The effective ingredient of Rhizoma Coptidis is Rhizoma Coptidis alkaloid, mainly is represented as berberine (BBR), accounts for 50% of total alkaloids; In addition, still contain fibrauretin, coptisine, 13-methyl-.psi.-coptisine., jateorhizine, magnoline etc., and 4 alkaloid sums such as berberine, fibrauretin, coptisine, jateorhizine account for 90% of total alkaloids.Experimental result shows that Rhizoma Coptidis alkaloid suppresses gluconeogenesis and promotes the sugar degraded and the generation blood sugar reducing function, BBR can active cell in AMPK vigor, increase cell to the consumption of glucose, reduce the effect such as insulin resistant, also there are some researches show give diabetic mice gavage various dose the berberine treatment after, serum islet have increase in various degree, and be directly proportional to dosage, show that berberine is improved the trend of diabetic mice serum insulin levels.
Three kinds of different Cortex Moris of the present invention, Semen Trigonellae seed, Rhizoma Coptidis play the blood sugar lowering effect in vivo with three kinds of different pharmacological mechanisms, with respect to certain single pharmacological mechanism effect, its blood sugar reducing function is remarkable, better efficacy, three kinds of extracts are complementary short mutually, synergism, both fundamentally strengthened whole body function, promote (even helping to repair) pancreatic function, and the secretion of the interior insulin of promotion body, reduce again the absorption to sugar, accelerate glucose metabolism, thereby control, reduce blood sugar level; Protect simultaneously, improve blood circulation, improve gastrointestinal movement, prevent and treat other concurrent diseases of diabetes.
Compared with prior art, advantage of the present invention is:
1, Chinese medicine composition compound for reducing blood suger of the present invention, raw material sources are in traditional Chinese medicine, and raw material is easy to get, and preparation is simple.
2, in Chinese medicine composition of the present invention, three kinds of Chinese medicines play the blood sugar lowering effect with different mechanism, aspect the various symptoms of improving diabetes and complication thereof, obvious effect is being arranged, the present invention forms compound for reducing blood suger with three kinds of Chinese medicines, said composition joins together to bring into play synergism with three kinds of mechanism, with respect to single Chinese medicine, its function of reducing blood sugar is remarkable, and can better prevent and treat the complication that diabetes cause.The following examples 4 related experiment data are fine illustrates the concertedness of this combination blood sugar reducing function.
The specific embodiment
The present invention is described further below in conjunction with embodiment.
Embodiment 1
Take: 6 parts of Cortex Mori, 3 parts, Semen Trigonellae seed, 3 parts of Rhizoma Coptidis
With the raw material of above-mentioned weight proportion, mix homogeneously adds the water of 10 times of weight, soaks 30 minutes, boils 30 minutes, takes out and keeps filtrate; Add again the water of 8 times of weight in filtering residue, soaked 20 minutes, boiled 20 minutes, keep filtrate; Merge 2 times filtrate, being concentrated into relative density is 1.05.
After filtrate let cool, first adding mass concentration was 30% lime cream, shakes up, and shook all, then added the ethanol precipitation, and the final concentration that makes ethanol is 50%, and supernatant is got in centrifugalize;
Supernatant is regulated pH to 3.0, passes through large pores cation exchange resin or the absorption of gel type cation exchanger resin except precipitation is rear, after impurity is removed in washing, be 2.5% ammonia eluting resin with mass concentration, collect the ammonia eluent, concentrate drying gets Chinese medicine composition of the present invention; According to the preparation technology of granule, namely get granule.
Embodiment 2
Take: 6 parts of Cortex Mori, 3 parts, Semen Trigonellae seed, 3 parts of Rhizoma Coptidis
With the raw material of above-mentioned weight proportion, mix homogeneously adds the water of 10 times of weight, soaks 20-30 minute, boils 20-30 minute, takes out and keeps filtrate; Add again the water of 8 times of weight in filtering residue, soaked 20-30 minute, boiled 20-30 minute, keep filtrate; Merge 2 times filtrate, being concentrated into relative density is 1.15.
After filtrate let cool, first adding mass concentration was 25% lime cream, shakes up, and shook all, then added the ethanol precipitation, and the final concentration that makes ethanol is 60%, and supernatant is got in centrifugalize;
Supernatant is regulated pH to 4.0, passes through large pores cation exchange resin or the absorption of gel type cation exchanger resin except precipitation is rear, after impurity is removed in washing, be 2% ammonia eluting resin with mass concentration, collect the ammonia eluent, concentrate drying gets Chinese medicine composition of the present invention; Wet method film-making technique according to tablet makes tablet.
Embodiment 3
Take: 6 parts of Cortex Mori, 3 parts, Semen Trigonellae seed, 3 parts of Rhizoma Coptidis
With the raw material of above-mentioned weight proportion, mix homogeneously adds the water of 10 times of weight, soaks 20-30 minute, boils 20-30 minute, takes out and keeps filtrate; Add again the water of 8 times of weight in filtering residue, soaked 20-30 minute, boiled 20-30 minute, keep filtrate; Merge 2 times filtrate, being concentrated into relative density is 1.10.
After filtrate let cool, first adding mass concentration was 15% lime cream, shakes up, and shook all, then added the ethanol precipitation, and the final concentration that makes ethanol is 55%, and supernatant is got in centrifugalize;
Supernatant is regulated pH to 3.0, passes through large pores cation exchange resin or the absorption of gel type cation exchanger resin except precipitation is rear, after impurity is removed in washing, be 1.5% ammonia eluting resin with mass concentration, collect the ammonia eluent, concentrate drying gets Chinese medicine composition of the present invention; Preparation process according to capsule makes capsule.
Embodiment 4
At the different blood sugar reducing functions of joining together aspect machine-processed, the below further introduces the conclusion that the present invention studies in every pharmacological basis research for one of proved inventive embodiment 1-3 described Chinese medicine composition:
One, this invention compositions is tested the diabetes rat carbohydrate tolerance that STZ induces
the Wistar rat body weight is 180～200g, tail vein injection STZ 50mg/kg after fasting, surveying the above person of blood glucose value 11.1mol/L after 72 hours tests, during grouping, each treated animal average blood sugar value is more or less the same in 6mo1/L, it is 7 groups that rat is divided into, namely organize on an empty stomach, matched group, the acarbose group, small dose group, middle dosage group, heavy dose of group, (Cortex Mori is soaked in the alcoholic solution of 8-12 times of 40%-95% the Cortex Mori extract group, after boiling water bath 2-3 hour, filter, after filtering residue extracts one time with the ethanol of 6 times 75% again with method, merging filtrate, centrifugal filtration, concentrated, spray drying namely gets Cortex Mori extract), equal overnight fasting, fill with normal saline except organizing on an empty stomach, other respectively organize equal gavage starch 5g/kg, while matched group gavage normal saline, acarbose group gavage acarbose 50mg/kg, small dose group gavage present composition 100mg/kg, middle dosage group gavage 200g/kg, heavy dose of group gavage 300mg/kg, Cortex Mori group gavage 200g/kg.After administration, 60min surveys blood sugar level, the results are shown in Table 1.
The diabetes rat carbohydrate tolerance impact that this Chinese medicine compound for reducing blood suger of table 1. is induced STZ
﹟ P＜0.01 v.s. organizes * P＜0.01 v.s. matched group on an empty stomach
Experimental data according to table 1, Chinese medicine composition of the present invention has the level of postprandial blood sugar that can reduce diabetic mice identical with acarbose, though action effect is not as good as acarbose, but can show the have some improvement ability of diabetic mice level of postprandial blood sugar of said composition tool; In said composition, the dosage group is better with respect to its ability that reduces the diabetic mice post-prandial glycemia of Cortex Mori extract group.
Two, the impact of present composition various dose on spontaneous hyperglycemia mouse blood sugar and serum insulin
The NOD mice on an empty stomach after 6h, is measured blood glucose, selects blood glucose value higher than 60 of the animals of 11.1mmol/L, is divided at random 6 groups, and gavage gives following medicine respectively: 1. matched group (distilled water); 2. glibenclamide group (50mg/kg); 3.-5. the compositions of various dose group,
Semen Trigonellae seed extract group (is got the Semen Trigonellae seed, is added the water of 10 times of weight, soaked 20-30 minute, boiled 20-30 minute, take out and keep filtrate; Add again the water of 8 times of weight in filtering residue, soaked 20-30 minute, boiled 20-30 minute, keep filtrate; Merge 2 times filtrate, drying with water bath is powder, makes the Semen Trigonellae water extract), low dose of gavage present composition 100mg/kg, middle dosage group gavage 200g/kg, heavy dose of group gavage 300mg/kg, Semen Trigonellae extract group 200 mg/kg.Empty stomach 6h before successive administration 14 days, administration in the 14th day, after administration, the 1h animal is got blood, measures the animal blood glucose value with happy one-tenth blood glucose monitoring system, uses the serum measured by radioimmunoassay insulin.The results are shown in Table 2-3.
Table 2 pharmaceutical composition is on the impact of spontaneous hyperglycemia mouse blood sugar (X ± SD)
Table 3 pharmaceutical composition is on the impact of spontaneous hyperglycemia mice serum insulin (X ± SD)
Compare * P＜0.05, * * P＜0.01 with model control group
Experimental data according to table 2-3, compound for reducing blood suger of the present invention can significantly reduce the NOD mouse blood sugar, and the blood sugar lowering ability is relevant with the content of said composition effective ingredient, content is high, the blood sugar lowering ability is more remarkable, and said composition also can improve the level of insulin in the NOD mice serum simultaneously, and also the active constituent content to said composition is relevant for the level of raising serum insulin, content is high, improves serum insulin levels also higher; This compound for reducing blood suger median dose group is compared with Semen Trigonellae seed extract group, and the blood sugar lowering ability is suitable, and the ability that improves serum insulin levels is also suitable.
Three, the impact of the present composition on insulin resistance rat
(1) foundation of type ii diabetes Rat model of insulin-resistant: after 70 SD male rat adaptabilities fed for 1 week, be divided at random modeling group (70) and blank group (10), blank group feed normal diet, modeling group feed high lipid food (being made by 12% Adeps Sus domestica, 48% normal feedstuff, 15% sugar, 10% Semen arachidis hypogaeae, 10% egg yolk, 5% salt and 0.03% Fel Sus domestica salt by the quality percentage composition).Feed (the 8th week) therebetween, the modeling group is by 25mg/Kg BW disposable celiac injection 0.25% STZ solution (being dissolved in citrate buffer solution); The isopyknic citrate buffer of blank group lumbar injection.After 10 weeks, detect each Mus fasting glucose and fasting insulin level, calculate insulin sensitivity index (ISI).
(2) grouping and administration: take insulin sensitivity index as screening index, screening modeling success rat is 70 in the modeling group, the Chinese medicine high dose group (10) that is divided at random present embodiment, dosage group in the Chinese medicine of present embodiment (10), the Chinese medicine low dose group of present embodiment (10), rosiglitazone group (10), model group (10), the Rhizoma Coptidis extract group (grinds to form fine powder with dried Rhizoma Coptidis, weigh, use the alcohol reflux Rhizoma Coptidis total alkaloids, 80% alcohol reflux 3 times, add for the first time 6 times of amount 80% ethanol, extract 2h, add for the second time 5 times of amount 80% ethanol, extract l.5h, add for the third time 4 times of amount 80% ethanol, extract 1.5h, merging filtrate, the concentrating under reduced pressure solvent evaporated, obtain extractum, be the total alkaloids extract of Rhizoma Coptidis) (10), blank group (10).Low dose group gavage said composition 100mg/kg of the present invention, middle dosage group gavage said composition 200mg/kg, high dose group gavage said composition 300 mg/kg, the rosiglitazone group is pressed 50mg/kg dosage gastric infusion, the Rhizoma Coptidis extract group is by 200 mg/kg dosage gavages, and blank group and model group gavage the normal saline 10mL/kg of equal volume every day.
(3) rat general status observation: the situations such as food ration, body weight, outward appearance and behavior variation of observing rat.Detect the serum insulin level.Use blood glucose meter and detect fasting glucose (FBG); Adopt radioactive immunoassay to measure serum insulin (FINS); Calculate simultaneously Insulin Sensitivity Index ISI=ln[1/ (FBG * FINS)].
Data SPSS software processes, the data of normal distribution is so that (x ± s) expression, the data of nonnormal distribution is carried out statistical analysis after all taking from right lognormal.
(4) experimental result:
The observation of rat general status: before modeling, each is organized, and activities in rats agility, fur are bright and clean, food ration, amount of drinking water, urine amount are all normal.After modeling, except the blank group, all the other rat aggregate performances are lethargy, bradykinesia, hair is withered and yellow unglazed, and food ration, amount of drinking water and urine amount increase.The Chinese medicine of present embodiment sees Table 4 to the impact of rat fasting blood-glucose (FBG), fasting insulin (FINS), insulin sensitivity index (ISI)
This blood-sugar lowering tcm drug of table 4 compositions is on rat FBG, FINS, and the impact of ISI (x ± s)
With the blank group Bi Jiao ﹟ P＜0.05 ， ﹟ ﹟ P＜0.01; Compare * P＜0.05, * * P＜0.01 with model group
According to the experimental data of table 4, compare with the blank group, model group rat FBG, FINS level raise, and ISI descends, and significant difference (P＜0.01) is arranged, and illustrates that insulin resistant model copies successfully.Compare with model group, each administration group rat FBG, FINS descend (P＜0.01), and ISI raises (P＜0.05) to some extent simultaneously, and difference has statistical significance.Result shows, the Chinese medicine of present embodiment can reduce FBG and the FINS of insulin resistance rat, rising ISI, the abnormal carbohydrate metabolism when improving insulin resistant; Said composition median dose group and Rhizoma Coptidis extract group relatively its FBG and FINS that reduces insulin resistance rat are more obvious, and rising ISI exponential quantity is large.
Claims (10)
1. a Chinese medicine composition, is characterized in that, it is after being mixed by the raw material of following weight portion, to get extracting solution through water extraction; Extracting solution is the lime cream of 5%-50% with adding mass concentration, after shaking up, then adds the ethanol precipitation, and the quality final concentration that makes ethanol is 40%-80%, and supernatant is got in centrifugalize; Supernatant by cationic exchange resin adsorption, is ammonia eluting, the collection ammonia eluent of 0.20%-5.0% with mass concentration, the extract that obtains after concentrate drying:
Cortex Mori 6-12 part;
Semen Trigonellae seed 3-6 part;
Rhizoma Coptidis 3-6 part.
2. Chinese medicine composition according to claim 1, is characterized in that, the weight portion of described raw material consists of:
Cortex Mori 6-9 part;
Semen Trigonellae seed 3-4.5 part;
Rhizoma Coptidis 3-4.5 part.
3. Chinese medicine composition according to claim 1, is characterized in that, the weight portion of described raw material consists of:
6 parts of Cortex Mori;
3 parts, Semen Trigonellae seed;
3 parts of Rhizoma Coptidis.
4. one of according to claim 1-3 described Chinese medicine compositions, is characterized in that, described Rhizoma Coptidis is Rhizoma Coptidis.
5. one of according to claim 1-3 described Chinese medicine compositions, is characterized in that, the dosage form of described Chinese medicine composition comprises powder, granule, tablet or capsule.
6. the preparation method of the described Chinese medicine composition of one of claim 1-5, is characterized in that, concrete steps are:
(1) according to the described weight portion proportioning of one of claim 1-5, with Cortex Mori, Semen Trigonellae seed and Rhizoma Coptidis mix homogeneously, get raw mix; Add the water with respect to raw mix 8-10 times weight, soaked 20-30 minute, then reflux 20-30 minute, filter, keep filtrate a; Add again the water of 8-10 times of weight in filtering residue, soaked 20-30 minute, then reflux 20-30 minute, filtrate b kept; Merging filtrate a and filtrate b get the filtrate mixture; It is 1.02-1.20 that the filtrate mixture is concentrated into relative density;
(2) first adding mass concentration in the filtrate mixture after concentrated is the lime cream of 5%-50%, shakes up, then adds the ethanol precipitation, and the quality final concentration that makes ethanol is 40%-80%, and supernatant is got in centrifugalize;
(3) supernatant is regulated pH to 2.0-5.0, pass through cationic exchange resin adsorption except precipitation is rear, after the washing remove impurity, be 0.20%-5.0% ammonia eluting resin with mass concentration, collect the ammonia eluent, concentrate drying namely gets Chinese medicine composition of the present invention.
7. the preparation method of Chinese medicine composition according to claim 6, is characterized in that, described in step (2), the mass concentration of lime cream is 15%-30%; The quality final concentration of described ethanol is 50%-60%.
8. the preparation method of Chinese medicine composition according to claim 6, is characterized in that, in step (3) with the sulfur acid for adjusting pH to 3.0-4.0.
9. the preparation method of Chinese medicine composition according to claim 6, it is characterized in that, the cation exchange resin described in step (3) is one or more in D001 type cation exchange resin, 001 * 4 type gel type resin, 001 * 7 type gel type resin and 001 * 1II type gel type resin.
10. the application of the described Chinese medicine composition of one of claim 1-5 in preparation prevention or treatment diabetes medicament.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078992A (en) * | 2014-05-23 | 2015-11-25 | 资元堂生物科技股份有限公司 | Application of isoquinoline alkaloid derivative in preparing medicine for promoting AMPK activity |
| CN107496603A (en) * | 2017-09-25 | 2017-12-22 | 首都医科大学 | A kind of Chinese medicine composition for treating IGR and preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102327324A (en) * | 2011-09-23 | 2012-01-25 | 华润三九医药股份有限公司 | Mulberry leaf total alkali extract and preparation method and application thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102327324A (en) * | 2011-09-23 | 2012-01-25 | 华润三九医药股份有限公司 | Mulberry leaf total alkali extract and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
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| 陈仁寿: "降糖中药的性效探析", 《中国中药杂志》, vol. 30, no. 12, 30 June 2005 (2005-06-30) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078992A (en) * | 2014-05-23 | 2015-11-25 | 资元堂生物科技股份有限公司 | Application of isoquinoline alkaloid derivative in preparing medicine for promoting AMPK activity |
| CN105078992B (en) * | 2014-05-23 | 2017-11-21 | 资元堂生物科技股份有限公司 | Isoquinoline alkaloids alkali derivant is used for the purposes for preparing the medicine for promoting AMPK activity |
| CN107496603A (en) * | 2017-09-25 | 2017-12-22 | 首都医科大学 | A kind of Chinese medicine composition for treating IGR and preparation method and application |
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| CN103099865B (en) | 2014-02-26 |
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