CN103060203A - Fungus and application thereof - Google Patents
Fungus and application thereof Download PDFInfo
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- CN103060203A CN103060203A CN2012105604225A CN201210560422A CN103060203A CN 103060203 A CN103060203 A CN 103060203A CN 2012105604225 A CN2012105604225 A CN 2012105604225A CN 201210560422 A CN201210560422 A CN 201210560422A CN 103060203 A CN103060203 A CN 103060203A
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Abstract
The invention relates to a fungus and an application thereof, and belongs to the technical field of biology. The fungus is obtained after a production bacterial strain and auxiliary materials go through preliminary screening, second screening, natural medium culturing and Cephalosporium acremonium purification culturing process. The production bacterial strain is Cephalosporium acremonium CTC001, and is preserved in China general microbiological culture collection center on February 16, 2012, and preservation number is CGMCC No.5793. The fungus has a function of improving Pu'er tea quality, and is applied to the production technology of the Pu'er tea. The fungus and the application thereof have the advantages that when CTC001 is used for the Pu'er tea mass production according to a certain proportion, tea polyphenols and theabrownins in the Pu'er tea can be controlled in a certain proportion, the processing time is greatly shortened, and therefore important role is played in stability and improvement of the Pu'er tea quality.
Description
Technical field:
The present invention relates to a kind of fungi and application thereof, belong to biological technical field.
Background technology:
As everyone knows, Acremonium (Acremonium) is found in ocean mud, the soil ulmin, and the report that Acremonium is arranged in the pure bacterial strain of Chinese caterpillar fungus is also arranged simultaneously.Cephalosporium acremonium is the important industrial microorganism of a class, and the cephalosporin of its generation is the main raw material of producing cephalosporins important intermediate 7-amino-cephalosporanic acid (7-ACA).Cephalosporins is that a class is efficient, the microbiotic of low toxicity, wide clinical application.
In the Leaf of Assam Tea large-scale production process, this fungus strain can be secreted enzyme and act on matrix.Under diastatic effect, the straight chain amylopectin in the raw material is degraded to dextrin and various low molecular sugar class, such as maltose, glucose etc., under the effect of proteolytic enzyme, stodgy macro-molecular protein is degraded to peptone, polypeptide and each seed amino acid.Under the effect of polygalacturonase, saccharifying enzyme, cellulase, make fermented substrate macromole insoluble substance be converted into the small-molecule substance that is dissolvable in water water, the formation of Leaf of Assam Tea quality is brought positive effect.
In the Leaf of Assam Tea research field, because the research of Leaf of Assam Tea is especially carried out in the research of long-term lacking subject from the angle of microorganism.And how by the research to microorganism in the yunnan puer tea processing, and the useful superior microorganism bacterial strain of screening is applied to the quality that promotes Leaf of Assam Tea in the production and there is not yet disclosed bibliographical information from Leaf of Assam Tea processing.
Summary of the invention:
The object of the invention is to from the angle of microorganism Leaf of Assam Tea be studied, a kind of fungi (cephalosporium acremonium) and application thereof that improves the Leaf of Assam Tea quality function that have is provided, play a role for promoting yunnan puer tea stay in grade and raising.
Fungi of the present invention, obtained after cephalosporium acremonium purifying cultivation operation is cultivated, reached to primary dcreening operation, multiple sieve, natural medium by production bacterial strain and auxiliary material, cephalosporium acremonium (Cephalosporium acyemonium) CTC001 is preserved in Chinese common micro-organisms culture presevation administrative center, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on February 16th, 2012.Preserving number: CGMCC No.5793.
The morphological feature of cephalosporium acremonium of the present invention (Cephalosporium acyemonium) CTC001 is:
Isolated strains is fine hair shape or cotton wool shape at the PDA substratum.Quality is wet or not wet, baby pink after the initial stage white, the little yellow of reverse side.
10-14 days diameters of this mushroom can reach 3-4cm, and mycelia just is white, the rear baby pink that often occurs, and fine hair shape or cotton wool shape, reverse side are slightly yellow.Examine under a microscope the aerial hyphae tool every the upright branch not of, conidiophore, without every, central authorities are thick and end is gradually thin, conidium is gathered into head on the conidiophore top, comprise 10～25 conidiums.Conidium ellipse or oblong.Chlamydospore club shape, light color.
The present invention obtains after cultivating conventional processes through known primary dcreening operation, multiple sieve, natural medium cultivation, cephalosporium acremonium purifying.
Cephalosporium acremonium of the present invention (Cephalosporium acyemonium) CTC001 fungi is improved the effect of Leaf of Assam Tea quality, is applied in the production technique of Leaf of Assam Tea.
The invention has the advantages that: the large-scale production process that CTC001 is used for according to a certain percentage Leaf of Assam Tea, tea-polyphenol in its Leaf of Assam Tea and theabrownin can be controlled at certain ratio, and significantly shortened process period, stabilizing and increasing of Leaf of Assam Tea quality played a significant role.
Embodiment:
The screening of embodiment 1:CTC001 bacterial classification
From the tea sample that collects, obtain 12 cephalosporium acremonium bacterial strains by known plate isolation (substratum with cephalosporium acremonium PDA substratum).The cephalosporium acremonium pure medium is: glucose 30g, NaNO
330g, K
3PO
41g, MgSO
40.25g, KCl0.25g, agar 15g, distilled water 1000ml, pH value 6.8, at 121 ° of C sterilization 30min.Again by calcium carbonate transparent circle method select can produce simultaneously the higher AG of enzymic activity, CMC, polygalacturonase No. 5 bacterial strains as test organisms.Screening method is as follows:
A. primary dcreening operation: the cephalosporium acremonium bacterial strain that is separated to is inoculated into substratum (agar 20g, glucose 20g, CaCO
325g, add water to 1000ml, PH6.5-7,30min sterilizes in 121 ° of C pressure kettle) on, cultivated three days in 30 ° of C.Principle be glucoamylase can be in growth with the conversion of glucose in the substratum for acid and be diffused in the substratum, with CaCO wherein
3Effect produces transparent circle.Selecting the bacterial strain that wherein transparent circle is large sieves for multiple.
B. sieve again: with the bottled 50ml substratum of 250ml triangle (glucose 50g, peptone 3g, (NH
4)
2HPO
40.4g, KH
2PO
40.2g, MgSO
47H
2O0.1g) aspergillus niger strain of access primary dcreening operation after the sterilization is in vibration shake-flask culture 3 days (30 ° of C).Then measure acidity with PHS-2 type acidometer.Three bottles of every strain bacterium inoculations.Select at last a strain and produce the maximum bacterial strain of acid as the zymogenic bacteria kind.
C. at last the bacterial classification inoculation of selecting (glucose 30g, wheat bran 10g, Semen Maydis powder 50g, peptone 10g, yeast extract paste 1.0g, adding distil water to 1000, ph value 6.5-7) to natural medium.Be put in 28 ° of C thermostat containers sooner or later that wave and culture filtered with aseptic filter paper on the platform aseptic super the work after 5 ~ 7 days, the mycelia on the aseptic results filter paper and the spore of aspergillus niger are put in 28 ° of C thermostat containers dry 24 hours, can be used for the inoculation fermentation test.
The production application of embodiment 2:CTC001
Undertaken damp and hot by traditional method gross tea, the inoculation weight ratio is that the CTC001 of 0.1-0.8% allows its fermentation in gross tea subsequently, when heap temperature rise to 30 ± 3oC, CTC001 begins to carry out metabolism, and along with the heap temperature further raises, secrete a large amount of glucoamylases, cellulase and polygalacturonase.These enzymes carry out oxidation, hydrolysis to the component content in the tealeaves.Wherein reducing sugar, soluble-carbohydrate and aquation pectin present ever-increasing trend, but since this moment tealeaves in except CTC001, also have other a large amount of microorganisms to begin to grow, polysaccharide hydrolysate is used for growth and breeding by this many microorganism and consumes, thus the content of soluble-carbohydrate when going out heap than the large production of Leaf of Assam Tea and simulation large all lacking of producing.Coarse-fibred content also lacking than conventional process in going out to pile the tea sample simultaneously, this explanation, inoculation CTC001 makes the process of its fermentation dark compared with large production and the large attenuation degree of producing of simulation in the gross tea raw material, and CTC001 can accelerate to form the Leaf of Assam Tea qualitative characteristics and shorten process period.
The Leaf of Assam Tea of behind inoculation CTC001, producing, its quality is significantly improved, and the tea polysaccharide content in the Leaf of Assam Tea is that 2-4%, theabrownin content are 10-12%, process period, more traditional pile-fermentation shortened 1/3, the Leaf of Assam Tea product of formation, and the soup look red is dense bright, the sweet cunning of flavour, unique Chen Xiang.
Claims (2)
1. fungi, obtained after cephalosporium acremonium purifying cultivation operation is cultivated, reached to primary dcreening operation, multiple sieve, natural medium by production bacterial strain and auxiliary material, it is characterized in that producing bacterial strain is cephalosporium acremonium (Cephalosporium acyemonium) CTC001, be preserved in Chinese common micro-organisms culture presevation administrative center on February 16th, 2012, preserving number: CGMCC No.5793.
2. the said fungi of claim 1 is applied in the production technique of Leaf of Assam Tea.
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| CN2012105604225A CN103060203A (en) | 2012-12-21 | 2012-12-21 | Fungus and application thereof |
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| CN2012105604225A CN103060203A (en) | 2012-12-21 | 2012-12-21 | Fungus and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055235A (en) * | 2018-08-20 | 2018-12-21 | 云南农业大学 | A kind of preparation of the inoculating microbes strain for Pu'er tea pile fermentation and its application method |
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| CN101974440A (en) * | 2010-05-26 | 2011-02-16 | 云南农业大学 | Candida parapsilosis fungus and application thereof in production of pu'er tea |
| CN102010831A (en) * | 2010-05-26 | 2011-04-13 | 云南农业大学 | Lovastatin-generating fungus and application thereof in production of Pu'er tea |
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2012
- 2012-12-21 CN CN2012105604225A patent/CN103060203A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974440A (en) * | 2010-05-26 | 2011-02-16 | 云南农业大学 | Candida parapsilosis fungus and application thereof in production of pu'er tea |
| CN102010831A (en) * | 2010-05-26 | 2011-04-13 | 云南农业大学 | Lovastatin-generating fungus and application thereof in production of Pu'er tea |
Non-Patent Citations (2)
| Title |
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| 岩燕: "顶头孢酶对普洱茶主要功能化学成分的影响", 《百度文库》 * |
| 陈华红等: "顶头孢霉对普洱茶品质的影响", 《食品科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055235A (en) * | 2018-08-20 | 2018-12-21 | 云南农业大学 | A kind of preparation of the inoculating microbes strain for Pu'er tea pile fermentation and its application method |
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Application publication date: 20130424 |