CN1298837C - Aspergillus niger fungus and its application in production of Pu'er tea - Google Patents
Aspergillus niger fungus and its application in production of Pu'er tea Download PDFInfo
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- CN1298837C CN1298837C CNB2005100109414A CN200510010941A CN1298837C CN 1298837 C CN1298837 C CN 1298837C CN B2005100109414 A CNB2005100109414 A CN B2005100109414A CN 200510010941 A CN200510010941 A CN 200510010941A CN 1298837 C CN1298837 C CN 1298837C
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Abstract
The present invention relates to an aspergillus niger fungus and an application thereof in the production of Pu'er tea, which belongs to the technical field of biology. The fungus with the function of enhancing the quality of Pu'er tea of the present invention is obtained by the processes of the primary traditional screening, secondary screening and culture in a natural medium of a producing strain and auxiliary materials, and the purification culture of aspergillus nigers; the present invention is characterized in that the producing stain is an aspergillus niger PAsp0501, the preservation date is May 16th, 2005, and the preservation number is CGMCC No. 1373. The fungus has the function of enhancing the quality of Pu'er tea, and can be applied to a production process of Pu'er tea; the present invention has the advantages that if the PAsp0501 is used for the mass production process of Pu'er tea according to certain proportions, Pu'er tea contains 2 to 4% of tea polysaccharide and 10 to 12% of theabrownin, and the fungus obviously enhances the quality of Pu'er tea, shortens processing time, and plays important roles in the stabilization and the enhancement of the quality of Pu'er tea.
Description
Technical field:
The present invention relates to a kind of aspergillus niger fungi and the application in Leaf of Assam Tea is produced thereof, belong to biological technical field.
Background technology:
As everyone knows, Aspergillus (Aspergillus) is found in leavened food morely, is the bacterial strain that a class is produced prozyme, can produce amylase, proteolytic enzyme, polygalacturonase, saccharifying enzyme, cellulase, phytase etc. and be used for industrial production.They can also produce the haemolysis enzyme and be used to eliminate artery and phlebothrombosis.Some fungus strain can produce multiple organic acid such as citric acid, oxysuccinic acid, fumaric acid etc.
In the Leaf of Assam Tea large-scale production process, this fungus strain can be secreted enzyme and act on matrix.Under diastatic effect, the straight chain amylopectin in the raw material is degraded to dextrin and various low molecule carbohydrate, as maltose, glucose etc., under the effect of proteolytic enzyme, stodgy macro-molecular protein is degraded to peptone, polypeptide and each seed amino acid.Under the effect of polygalacturonase, saccharifying enzyme, cellulase, make fermented substrate macromole insoluble substance be converted into the small-molecule substance that is dissolvable in water water, the formation of Leaf of Assam Tea quality is brought positive effect.
In the Leaf of Assam Tea research field, because the research of Leaf of Assam Tea is especially carried out in the research of long-term lacking subject from the angle of microorganism.And how by the research to microorganism in the yunnan puer tea processing, and the useful superior microorganism bacterial strain of screening is applied to the quality that promotes Leaf of Assam Tea in the production and does not see disclosed bibliographical information as yet from Leaf of Assam Tea processing.
Summary of the invention:
The objective of the invention is Leaf of Assam Tea to be studied, for manufacturing enterprise provides high-quality Leaf of Assam Tea useful bacterium source from the angle of microorganism.Another object of the present invention is by the ratio of control probiotics in Leaf of Assam Tea is produced, and plays a role for promoting yunnan puer tea stay in grade and raising.
Aspergillus niger fungi of the present invention, obtain after aspergillus niger purifying cultivation operation is cultivated, reached to primary dcreening operation, multiple sieve, natural medium by production bacterial strain and auxiliary material, the production bacterial strain is aspergillus niger (Aspergillus niger) PAsp0501, be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, the depositary institution address: Beijing is No. 13, one in Zhong Guan-cun, Haidian District north, preservation date: on May 16th, 2005, preserving number: CGMCC No.1373.
The morphological feature of aspergillus niger of the present invention (Aspergillus niger) PAsp0501 is:
This fungus grown is limited to slightly, and diameter can reach 2.5-3cm in 10-14 days, and mycelia just is a white, yellow area usually occurs, the heavy fleece shape, and black, colourless on the contrary or middle body is tawny slightly.Examine under a microscope conidial head when children sphere, fade to radiation or be cleaved into the column form object of several radiation, be generally 1-3mm, diameter 15-20 μ m, wall thickness is smooth.The spherical general diameter 45-75 μ m of top capsule.The stigma bilayer, certainly the top capsule comprehensive give birth to, brown, the general 20-30 of metulae * 5-6 μ m, the elder can reach 60-70 μ m, wide 8-10 μ m, the tool tabula that has, the conidium sphere, the most 4-5 μ of diameter m, coarse.
The present invention obtains after conventional processes such as known primary dcreening operation, multiple sieve, natural medium cultivation, the cultivation of aspergillus niger purifying.
Aspergillus niger of the present invention (Aspergillus niger) PAsp0501 fungi has the effect that improves the Leaf of Assam Tea quality, can be applicable in the production technique of Leaf of Assam Tea.
The invention has the advantages that: the large-scale production process that PAsp0501 is used for according to a certain percentage Leaf of Assam Tea, tea polysaccharide content in its Leaf of Assam Tea is that 2-4%, theabrownin content are 10-12%, the quality of Leaf of Assam Tea is significantly improved, and shortened process period, stabilizing and increasing of Leaf of Assam Tea quality played a significant role.
Embodiment:
The screening of embodiment 1:PAsp0501 bacterial classification
From the tea sample that collects, obtain 48 strain Aspergillus niger strains by known plate isolation (substratum with aspergillus niger separation and purification substratum).Aspergillus niger purifying substratum is: glucose 30g, NaNO
330g, K
3PO
41g, MgSO
40.25g, KCL0.25g, agar 15g, distilled water 1000ml, ph value 6.8, at 121 ℃ of sterilization 30min.Again by lime carbonate transparent circle method select can produce the higher AG of enzymic activity, CMC, polygalacturonase simultaneously No. 5 bacterial strains as test organisms.Screening method is as follows:
A. primary dcreening operation: the Aspergillus niger strain that is separated to is inoculated into substratum (agar 20g, glucose 20g, CaCO
325g, add water to 1000ml, PH6.5-7,30min sterilizes in 121 ℃ of pressure kettle) on, cultivated three days in 30 ℃.Principle be glucoamylase can be in growth with the conversion of glucose in the substratum for acid and be diffused in the substratum, with CaCO wherein
3Effect produces transparent circle.Selecting the bacterial strain that wherein transparent circle is big sieves for multiple.
B. sieve again: with the bottled 50ml substratum of 250ml triangle (glucose 50g, peptone 3g, (NH
4)
2HPO
40.4g, KH
2PO
40.2g, MgSO
47H
2O0.1g) aspergillus niger strain of primary dcreening operation is inserted in the sterilization back, in vibration shake-flask culture 3 days (30 ℃).Measure acidity with PHS-2 type acidometer then.Three bottles of every strain bacterium inoculations.Select a strain at last and produce the maximum bacterial strain of acid as the zymogenic bacteria kind.
C. at last the bacterial classification inoculation of selecting (glucose 30g, wheat bran 10g, Semen Maydis powder 50g, peptone 10g, yeast extract paste 1.0g, adding distil water to 1000, ph value 6.5-7) to natural medium.Be put in 28 ℃ of thermostat containers sooner or later that wave and culture filtered with aseptic filter paper on the platform aseptic super the work after 5～7 days, the mycelia on the aseptic results filter paper and the spore of aspergillus niger are put into that drying promptly can be used for the inoculation fermentation test in 28 ℃ of thermostat containers.
The production application of embodiment 2:PAsp0501
Undertaken damp and hot by traditional method gross tea, the inoculation weight ratio is that the PAsp0501 of 0.1-0.8% allows its fermentation in gross tea subsequently, when heap temperature rise to 30 ± 3 ℃, PAsp0501 begins to carry out metabolism, and, secrete a large amount of glucoamylases, cellulase and polygalacturonase etc. along with the heap temperature further raises.These enzymes carry out oxidation, hydrolysis to the component content in the tealeaves.Wherein reducing sugar, soluble-carbohydrate and aquation pectin present ever-increasing trend, but since this moment tealeaves in except that PAsp0501, also have other a large amount of microorganisms to begin to grow, polysaccharide hydrolysate is used for growth and breeding by this many microorganism and consumes, thus the content of soluble-carbohydrate when going out heap than the big production of Leaf of Assam Tea and simulation big all lacking of producing.Coarse-fibred content also lacking in going out to pile the tea sample simultaneously than conventional process, this explanation, inoculation PAsp0501 makes the process of its fermentation dark compared with big production and the big attenuation degree of producing of simulation in the gross tea raw material, and PAsp0501 can quicken to form the Leaf of Assam Tea qualitative characteristics and shorten process period.
The Leaf of Assam Tea of behind inoculation PAsp0501, producing, its quality is significantly improved, and the tea polysaccharide content in the Leaf of Assam Tea is that 2-4%, theabrownin content are 10-12%, the Leaf of Assam Tea product of formation, the soup look red is dense bright, the sweet cunning of flavour, unique Chen Xiang.
Claims (4)
1, a kind of aspergillus niger, obtain after aspergillus niger purifying cultivation operation is cultivated, reached to primary dcreening operation, multiple sieve, natural medium by the production bacterial strain, it is characterized in that producing bacterial strain is aspergillus niger (Aspergillus niger) PAsp0501, be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, depositary institution address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, preservation date: on May 16th, 2005, preserving number: CGMCC No.1373.
2, a kind of aspergillus niger is characterized in that it is aspergillus niger (Aspergillus niger) PAsp0501, preserving number: CGMCC No.1373.
3, claim 1 or 2 said aspergillus nigers is characterized in that this aspergillus niger has the effect that improves the Leaf of Assam Tea quality, can be applied in the production technique of Leaf of Assam Tea.
4, the application of claim 1 or 2 said aspergillus nigers is characterized in that this aspergillus niger uses in the production technique of Leaf of Assam Tea.
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| CNB2005100109414A CN1298837C (en) | 2005-07-28 | 2005-07-28 | Aspergillus niger fungus and its application in production of Pu'er tea |
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| CNB2005100109414A CN1298837C (en) | 2005-07-28 | 2005-07-28 | Aspergillus niger fungus and its application in production of Pu'er tea |
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| CN1752202A CN1752202A (en) | 2006-03-29 |
| CN1298837C true CN1298837C (en) | 2007-02-07 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100500013C (en) * | 2006-07-26 | 2009-06-17 | 邓雅然 | Ageing processing method for pu'er tea |
| CN101142953B (en) * | 2007-10-29 | 2010-07-14 | 云南省农业科学院茶叶研究所 | Pu'er tea processing method by inoculated fungi microbe |
| CN101427717B (en) * | 2008-12-10 | 2011-10-19 | 云南农业大学 | Method for preparing pu'er tea fermentation mother culture |
| CN101974440B (en) * | 2010-05-26 | 2012-05-30 | 云南农业大学 | Candida parapsilosis fungus and application thereof in production of pu'er tea |
| CN105602853B (en) * | 2014-11-24 | 2019-04-23 | 勐海茶业有限责任公司 | One plant of angstrom of Mo Senluosashi bacterial strain and its application in Pu'er tea production |
| CN109699766A (en) * | 2017-10-25 | 2019-05-03 | 勐海茶业有限责任公司 | The method for preparing fermented tea, the fermented tea prepared with this method and its application |
| CN108998393A (en) * | 2018-07-13 | 2018-12-14 | 云南中茶茶业有限公司 | One bacillus subtilis and its preparation have the Pu'er tea method for adjusting function of intestinal canal |
| CN111471599A (en) * | 2020-05-04 | 2020-07-31 | 上海聚茂塑胶制品有限公司 | Biodiesel and preparation process thereof |
| CN112515008A (en) * | 2020-12-09 | 2021-03-19 | 普洱学院 | Method for improving quality of black tea through aspergillus niger fungus |
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| US4885249A (en) * | 1984-12-05 | 1989-12-05 | Allelix, Inc. | Aspergillus niger transformation system |
| CN1038010A (en) * | 1988-05-29 | 1989-12-20 | 中山大学 | Method with tealeaves inoculated fungi system slim tea |
| CN1059451A (en) * | 1990-09-04 | 1992-03-18 | 湖南进出口商品检验局 | Artificial method of inoculing green aspergillus onto brick tea |
| JPH07303474A (en) * | 1994-05-11 | 1995-11-21 | Unitika Ltd | Aspergillus nigger sbo-30 strain |
| CN1291647A (en) * | 2000-11-17 | 2001-04-18 | 中国科学院上海有机化学研究所 | Aspergillus niger strain and its culture method and application |
| CN1385520A (en) * | 2002-04-19 | 2002-12-18 | 浙江省农业科学院 | Aspergillus niger strains and use thereof |
| CN1421523A (en) * | 2002-07-22 | 2003-06-04 | 江南大学 | Aspergillus niger and its microbial conversion process of producing vanillic acid and vanillic aldehyde |
| CN1465694A (en) * | 2002-06-20 | 2004-01-07 | 中国科学院大连化学物理研究所 | Aspergillus niger and method for preparing rare low polarity ginsenoside by alcoholysis of ginsenoside using same |
-
2005
- 2005-07-28 CN CNB2005100109414A patent/CN1298837C/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4885249A (en) * | 1984-12-05 | 1989-12-05 | Allelix, Inc. | Aspergillus niger transformation system |
| CN1038010A (en) * | 1988-05-29 | 1989-12-20 | 中山大学 | Method with tealeaves inoculated fungi system slim tea |
| CN1059451A (en) * | 1990-09-04 | 1992-03-18 | 湖南进出口商品检验局 | Artificial method of inoculing green aspergillus onto brick tea |
| JPH07303474A (en) * | 1994-05-11 | 1995-11-21 | Unitika Ltd | Aspergillus nigger sbo-30 strain |
| CN1291647A (en) * | 2000-11-17 | 2001-04-18 | 中国科学院上海有机化学研究所 | Aspergillus niger strain and its culture method and application |
| CN1385520A (en) * | 2002-04-19 | 2002-12-18 | 浙江省农业科学院 | Aspergillus niger strains and use thereof |
| CN1465694A (en) * | 2002-06-20 | 2004-01-07 | 中国科学院大连化学物理研究所 | Aspergillus niger and method for preparing rare low polarity ginsenoside by alcoholysis of ginsenoside using same |
| CN1421523A (en) * | 2002-07-22 | 2003-06-04 | 江南大学 | Aspergillus niger and its microbial conversion process of producing vanillic acid and vanillic aldehyde |
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| CN1752202A (en) | 2006-03-29 |
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Assignee: Menghai Tea Industry Co., Ltd. Assignor: Mi Ming Contract record no.: 2010530000046 Denomination of invention: Aspergillus niger fungus and its application in production of Pu'er tea Granted publication date: 20070207 License type: Exclusive License Open date: 20060329 Record date: 20100927 |
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Granted publication date: 20070207 Termination date: 20120728 |