CN103045570B - Simple method for replenishing cellulosic raw material reversely - Google Patents
Simple method for replenishing cellulosic raw material reversely Download PDFInfo
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- CN103045570B CN103045570B CN201310014166.4A CN201310014166A CN103045570B CN 103045570 B CN103045570 B CN 103045570B CN 201310014166 A CN201310014166 A CN 201310014166A CN 103045570 B CN103045570 B CN 103045570B
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- raw material
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- cellulose raw
- feed supplement
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- 239000002994 raw material Substances 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000000855 fermentation Methods 0.000 claims abstract description 43
- 230000004151 fermentation Effects 0.000 claims abstract description 40
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 229920002678 cellulose Polymers 0.000 claims description 39
- 239000001913 cellulose Substances 0.000 claims description 39
- 239000006052 feed supplement Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 22
- 239000010902 straw Substances 0.000 claims description 13
- 239000002028 Biomass Substances 0.000 claims description 4
- 230000000994 depressogenic effect Effects 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 241000609240 Ambelania acida Species 0.000 claims description 2
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 2
- 244000105624 Arachis hypogaea Species 0.000 claims description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 2
- 235000018262 Arachis monticola Nutrition 0.000 claims description 2
- 229920000742 Cotton Polymers 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 244000020551 Helianthus annuus Species 0.000 claims description 2
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 2
- 241000878006 Miscanthus sinensis Species 0.000 claims description 2
- 241001520808 Panicum virgatum Species 0.000 claims description 2
- 235000014676 Phragmites communis Nutrition 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 239000010905 bagasse Substances 0.000 claims description 2
- 239000007799 cork Substances 0.000 claims description 2
- 238000000354 decomposition reaction Methods 0.000 claims description 2
- 238000006073 displacement reaction Methods 0.000 claims description 2
- 239000011121 hardwood Substances 0.000 claims description 2
- 235000020232 peanut Nutrition 0.000 claims description 2
- 230000003252 repetitive effect Effects 0.000 claims description 2
- 239000004753 textile Substances 0.000 claims description 2
- 239000002023 wood Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 239000012978 lignocellulosic material Substances 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000006180 nutrition needs Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a simple method for replenishing a cellulosic raw material reversely. In order to solve the problem that the liquidity characteristic of a fermentation liquor is enhanced because the cellulosic raw material is dissolved as being decomposed and utilized by fermentation strains during a fermentation process so as to cause a fermentation liquor to become thin gradually, a sterile pipeline is utilized to transfer the fermentation liquor with good liquidity in a fermentation jar into the other connected fermentation jar in which the cellulosic raw material is required to be replenished and a sterilization treatment is carried out, so that the cellulosic carbon source in the fermentation liquor reaches a higher concentration by transferring the fermentation liquor repeatedly, i.e. the fermentation is carried out by utilizing the cellulosic raw material with the high concentration. According to the method, the problem that the majority of the cellulosic raw materials shaped like a solid rod or a ball without water solubility cannot be replenished conveniently during the traditional cellulosic raw material replenishing process is solved; the defect of a high requirement to equipment is overcome; and the high-concentration fermentation by replenishing the cellulosic raw material in a gradual increasing manner is realized.
Description
Technical field
The invention belongs to enzymic fermentation field, specifically, relate to a kind of easy reverse method of adding cellulose raw material.
Technical background
Mierocrystalline cellulose is the poly-polysaccharide molecule of height being formed by connecting by β-Isosorbide-5-Nitrae-glycosidic link by Glucopyranose.Cellulose raw material refers to that main component is the general name of a cellulosic class material.Utilize in process because cellulosic high polymerization property causes its solubleness extreme difference in water at cellulose raw material, substantially exist with the form of solid.In the fermentation field taking fibrous material as carbon source, the cellulose raw material of high density can cause soltion viscosity excessive, the equipment requirements such as stirring is crossed to high energy consumption and require excessively, thereby often adopts process fed-batch fermentation in high density cellulose raw material fermenting process.By early stage lower concentration cellulose raw material to meet nutritional needs in microorganism early stage, in process, add continuously or in batches the effect that reaches high gravity fermentation.In water, then carry out conventional solution and add because the low water solubility of cellulosic material causes adding can not being directly dissolved in process, after many employings are ground into powder at present, wiring solution-forming stream adds, in addition add and also have relevant report by high-pressure liquefaction or by the equipment of particular design, above method in use high to equipment requirements, Expenses Cost is high, not too easily promote.
Summary of the invention
The object of the invention is: a kind of easy reverse method of adding cellulose raw material is provided, simple to operate, promote easily, reduce the requirement to equipment.
Technical solution of the present invention is: can be fermented during the fermentation bacterial strain decomposition utilization according to cellulose raw material and dissolve, cause the fermented liquid characteristic that thinning mobility strengthens gradually, the fermented liquid of good fluidity in fermentor tank is transferred to by aseptic pipeline in another connected fermentor tank of the process sterilising treatment that the cellulose raw material that will add is housed, make the level of cellulosic carbon source in fermented liquid reach higher concentration by repetitive displacement fermented liquid, realize the fermentation of high density cellulose raw material; The step of specifically adding cellulose raw material is as follows:
(1) prepare two connected tanks, one of them is fermentor tank, and another is feed supplement tank;
(2) when cellulose raw material in fermentor tank is degraded flow of solution good time substantially, in feed supplement tank, add a certain amount of cellulose raw material not add water, 100 ~ 115 DEG C of sterilizing 15 ~ 20min, after sterilizing, pass into sterile air and be cooled to leavening temperature, at the feed supplement tank sterilizing connecting tube high-temperature sterilization 10min of logical steam to two jar simultaneously;
(3) fermented liquid in fermentor tank pressurizes to be depressed in the feed supplement tank of adding cellulose raw material through sterilizing by sterile air and ferments, again original fermentor tank is cleaned to the vacant feed supplement tank as next circulation, and feed supplement tank ferments as new fermentor tank;
(4) repeat 2,3 steps, make the level of cellulosic carbon source in fermented liquid reach higher concentration by shifting fermented liquid, until reach the concentration of the cellulose raw material of appointment.
Wherein, described cellulose raw material is or the combination of several in agricultural crop straw, careless class, forest biomass; Described agricultural crop straw is one or more the combination in straw, wheat straw, Cotton Stalk, Rape Straw, cornstalk, corn cob, soybean stalk stem, peanut stalk stem, Sunflower Stalk, bagasse; Described careless class is one or more the combination in Chinese silvergrass, switchgrass, reed; Described forest biomass are one or more the combination in cork, hardwood, wood sawdust.
Wherein, the relevant raw material of cellulosic that described cellulose raw material produces for papermaking, textile industry.
Wherein, the lower concentration of initial fermentation is to determine there is no special specified requirement according to the number of times of adding of fermentation final concentration and fermentation design.
Wherein, described fermentation is liquid fermenting, includes aerobe fermentation and anaerobic fermentation.
Advantage of the present invention is: the mode that adopts reverse thinking, between two tanks that are connected, mutually shift fermented liquid, the reverse fermented liquid by good fluidity is transferred to cellulose raw material relaying supervention ferment, realize progressively increasing of cellulose raw material concentration in fermenting process, avoid in traditional fibre raw material adding procedure that water-fast solid is shaft-like or granulated substance is brought is inconvenient to add because cellulose raw material mostly is, subduction equipment requirements high drawback, realizes cellulose raw material progressively increase feed supplement and high gravity fermentation.
Brief description of the drawings
Fig. 1 is feed supplement apparatus sketch of the present invention.
Fig. 2 is feed supplement schematic flow sheet of the present invention.
Embodiment
As shown in Figure 1, two connected tanks, one of them is fermentor tank, another is feed supplement tank; As shown in Figure 2, fermentation proceeds to the stage of adding, and fermented liquid is depressed into feed supplement tank by connecting tube, as the solid line of Fig. 2 indicates, next feed supplement pushes back fermentor tank again by feed supplement tank, as Fig. 2 dotted line indicates, the feed supplement process that repeats solid line, dotted line, reaches the final concentration of adding.
Below in conjunction with specific embodiment, the present invention is described in further detail.It will be appreciated by those skilled in the art that these embodiment just in order to demonstrate the invention, but not limit the scope of the invention by any way.
Cellulose raw material in embodiment adopts straw, and humidity is 20% left and right, and mean length is 2 centimetres of left and right; Pre-treatment is directly 2 kilograms of straw to be placed in to the pre-treatment pressurized vessel of 50 liters, difference pre-treatment 20,10,5 and 0 minutes under the wet-hot steam of 200 DEG C, pretreated stalk is pulverized after drying, and obtains the pretreated lignocellulose after pulverizing by 200 object sieves.
Embodiment 1: with final concentration 6%(w/v) lignocellulosic material aerobic fermentation production of cellulose enzyme
Taking the preprocessing straw of 800g as C source, add Mandles minimal medium (corn steep liquor (CSL) 3g/L, (NH4)
2sO
41.4 g/L, KH
2pO
42 g/L, MgSO
47H2O 0.3g/L, FeSO
47H
2o 5mg/L, ZnSO
47H
2o 1.4mg/L, MnSO
47H
2o 1.6mg/L, CoCl
26H
2o 2mg/L, CaCl
20.01g/L) be settled to 40L in the fermentor tank of 50L, 121 DEG C of sterilizing 20min, wait to be cooled to 30 DEG C of seed suspensions with the Rutc30 of 10% inoculum size inoculation culture 48h, and temperature is 30 DEG C, melt oxygen is not less than 20% fermentation; Fermentation proceeds to 48h, in the empty 50L feed supplement tank being connected with fermentor tank, add the cellulose raw material of 800g, 115 DEG C of sterilizing 20min pass into sterile air and are cooled to outlet air temperature at 30 DEG C, in the feed supplement tank that goes out, use the connecting tube of two tanks of high pressure steam sterilization also cooling, in the time that the temperature of feed supplement tank and the temperature of connecting tube are all down to 30 DEG C, pressurize to the fermentor tank fermenting and the controlled fermentation condition in feed supplement tank that is depressed into continues to ferment; Treat that fermentation proceeds to the lignocellulose process of adding fermentation more than 72h recirculation, the cellulosic final concentration in final fermentor tank reaches 6% (w/v), and the work of FPA enzyme reaches 3.1u/ml.
Embodiment 2: adopt SSF zymotechnique to produce alcohol with the lignocellulosic material of 40% (w/v)
Take lignocellulosic material 40kg, magnesium sulfate 100g, ammonium sulfate 400g, potassium primary phosphate 100g add in 250L fermentor tank, 110 DEG C of sterilizing 10min, pass into sterile air and be cooled to 50 DEG C of enzyme liquid 150L(FPA enzyme 3u/ml of being alive that add through degerming), after stirring 1h, be cooled to 30 DEG C, be cultured to the yeast saccharomyces cerevisiae of logarithmic phase with 1% inoculum size access, anaerobically fermenting; When fermentation proceeds to 24h, in the 250L feed supplement tank being connected, add the 40kg cellulose raw material 110 DEG C of sterilizing 10min that do not add water, the connected connecting tube of sterilizing simultaneously, subject to sterilizationly completely be cooled to 30 DEG C and with sterile air pressurization, the feed liquid in fermentor tank be transferred in the feed supplement tank of adding cellulose raw material and continue anaerobic fermentation, after fermentation ends, ethanol concn is up to 81.3g/L.
Example 1 and example 2 are respectively the present invention produces enzymic fermentation and anaerobic zymamsis application at aerobic, shift and reach high gravity fermentation by reverse simple feed liquid, reduce the requirement to adding equipment, ensured the simple of operating process simultaneously, be easy to large-scale promotion.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (2)
1. the easy reverse method of adding cellulose raw material, can be fermented during the fermentation bacterial strain decomposition utilization according to cellulose raw material dissolves, cause the fermented liquid characteristic that thinning mobility strengthens gradually, the fermented liquid of good fluidity in fermentor tank is transferred to by aseptic pipeline in another connected fermentor tank of the process sterilising treatment that the cellulose raw material that will add is housed, make the level of cellulosic carbon source in fermented liquid reach higher concentration by repetitive displacement fermented liquid, realize the fermentation of high density cellulose raw material; The step of specifically adding cellulose raw material is as follows: (1) prepares two connected tanks, and one of them is fermentor tank, and another is feed supplement tank; (2) when cellulose raw material in fermentor tank is degraded flow of solution good time substantially, in feed supplement tank, add a certain amount of cellulose raw material not add water, 100 ~ 115 DEG C of sterilizing 15 ~ 20min, after sterilizing, pass into sterile air and be cooled to leavening temperature, at the feed supplement tank sterilizing connecting tube high-temperature sterilization 10min of logical steam to two jar simultaneously; (3) fermented liquid in fermentor tank pressurizes to be depressed in the feed supplement tank of adding cellulose raw material through sterilizing by sterile air and ferments, again original fermentor tank is cleaned to the vacant feed supplement tank as next circulation, and feed supplement tank ferments as new fermentor tank; (4) repeat 2,3 steps, make the level of cellulosic carbon source in fermented liquid reach higher concentration by shifting fermented liquid, until reach the concentration of the cellulose raw material of appointment; Wherein, one or the combination of several in the relevant raw material of described cellulose raw material produces with textile industry for agricultural crop straw, careless class, forest biomass, papermaking cellulosic; Described agricultural crop straw is one or more the combination in straw, wheat straw, Cotton Stalk, Rape Straw, cornstalk, corn cob, soybean stalk stem, peanut stalk stem, Sunflower Stalk, bagasse; Described careless class is one or more the combination in Chinese silvergrass, switchgrass, reed; Described forest biomass are one or more the combination in cork, hardwood, wood sawdust; It is characterized in that: the lower concentration of initial fermentation is to determine there is no special specified requirement according to the number of times of adding of fermentation final concentration and fermentation design.
2. the easy reverse method of adding cellulose raw material according to claim 1, is characterized in that: described fermentation is liquid fermenting, includes aerobe fermentation and anaerobic fermentation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310014166.4A CN103045570B (en) | 2013-01-15 | 2013-01-15 | Simple method for replenishing cellulosic raw material reversely |
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| CN103045570B true CN103045570B (en) | 2014-06-11 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772877A (en) * | 2005-09-28 | 2006-05-17 | 哈尔滨工业大学 | Continuous flow culture method of industrial biological hydrogen preparing spawn and biological hydrogen preparing system reinforcing method |
| CN101338325A (en) * | 2008-08-28 | 2009-01-07 | 河南富城生态能源科技有限公司 | Process for producing methane by dry-type fermentation of stalks |
| CN202157063U (en) * | 2011-06-30 | 2012-03-07 | 苏柯汉（潍坊）生物工程有限公司 | Material filling device for fermenter |
-
2013
- 2013-01-15 CN CN201310014166.4A patent/CN103045570B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772877A (en) * | 2005-09-28 | 2006-05-17 | 哈尔滨工业大学 | Continuous flow culture method of industrial biological hydrogen preparing spawn and biological hydrogen preparing system reinforcing method |
| CN101338325A (en) * | 2008-08-28 | 2009-01-07 | 河南富城生态能源科技有限公司 | Process for producing methane by dry-type fermentation of stalks |
| CN202157063U (en) * | 2011-06-30 | 2012-03-07 | 苏柯汉（潍坊）生物工程有限公司 | Material filling device for fermenter |
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| CN103045570A (en) | 2013-04-17 |
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