CN103018433A - Rapid elisa plate incubator - Google Patents
Rapid elisa plate incubator Download PDFInfo
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- CN103018433A CN103018433A CN2011102898947A CN201110289894A CN103018433A CN 103018433 A CN103018433 A CN 103018433A CN 2011102898947 A CN2011102898947 A CN 2011102898947A CN 201110289894 A CN201110289894 A CN 201110289894A CN 103018433 A CN103018433 A CN 103018433A
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- elisa plate
- temperature
- constant temperature
- incubation
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- 238000011534 incubation Methods 0.000 claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 238000002965 ELISA Methods 0.000 claims description 46
- 238000000034 method Methods 0.000 abstract description 5
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 238000003018 immunoassay Methods 0.000 abstract 1
- 238000002558 medical inspection Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 230000012447 hatching Effects 0.000 description 6
- 238000003556 assay Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Abstract
The invention discloses a rapid elisa plate incubator, belonging to electronic medical inspection instruments and being used for rapid incubation in enzyme immunoassay tests. In a conventional incubation method, an elisa plate is put in a constant temperature water-bath tank, and the temperature of a constant temperature line (3) of the water-bath tank is normally 37 DEG C, which is too small in temperature difference with the elisa plate at normal temperature state, so that the heating curve (4) of the elisa plate is very gentle. According to the rapid incubator, a modern temperature control and monitoring technique is adopted, and a high temperature environment (1) in an incubation room is created in an early period heating way, so that the elisa plate is rapidly heated (2) as the temperature difference between the elisa plate and the incubation room is increased. When the temperature of the elisa plate is approximate to a constant temperature point C, the temperature in the incubation room rapidly drops to the constant temperature point from the highest point B to be at a constant temperature, and the elisa plate is incubated under the condition that the constant temperatures of the elisa plate and the incubation room are approximate to the same, so that the heating time is greatly shortened, and the purpose of rapid incubation is achieved.
Description
Technical field
The present invention relates to a kind of quick couveuse of ELISA Plate of electron medical treatment testing instruments, particularly enzyme linked immune assay.
Background technology
Existing medical test enzyme linked immune assay need to be hatched in constant water bath box for a long time to ELISA Plate, and is consuming time more than 1 hour.And each taking laying board, all the essential factor temperature variation of unpacking and causing has influence on normally hatching of other ELISA Plate, for this reason, whenever opens case one time, and all very important person's suitable increase point incubation time of being more or less affects test effect.
Existing methodical major defect is: 1, and incubation time is long, and generalized case patient can not obtain assay the same day, need be subjected to come and go the torment of hospital; The doctor can not understand the state of an illness as early as possible, and is unfavorable to diagnose and treat diseases.2, power consumption is large, wants continuous heat tracing because of water bath, and the power of electric heater is larger, and irrelevant with the ELISA Plate quantity in the case, when particularly amount of inspection is few, more seems extremely uneconomical.3, water bath does not have timing device, if repeatedly pack ELISA Plate into, and timing, resolution difficulty.
Summary of the invention
The deficiency that exists in order to overcome existing water bath is utilized modern temperature control and monitoring technology, has invented the quick couveuse of a kind of ELISA Plate, and the time spent only is about 1/4 of water bath, and power consumption is low, hatches to finish the automatic alarm prompting.
The technical solution adopted in the present invention is: when hatching in constant water bath box owing to ELISA Plate, because the constant temperature point of water bath is too little with the ELISA Plate temperature difference of normal temperature state, so the ELISA Plate heating curve is extremely mild.The present invention then adopts heat early stage and brings up a camera incubata hot environment, makes the temperature difference increase of ELISA Plate and camera incubata and is rapidly heated.When constant temperature point that the temperature of ELISA Plate is hatched near the best, this moment, the temperature of camera incubata also dropped to rapidly constant temperature point constant temperature, greatly shortened the heating-up time of ELISA Plate, thereby reached the purpose of hatching fast.
Owing to shortened incubation time greatly, can carry out convenient, efficiently individuality, hatch on a small quantity, do not hatch and do not resemble need to raise the water bath to concentrate after the some.Because assay can in time be obtained, be conducive to diagnosis of disease.Obtain monitoring owing to hatch room temperature, ELISA Plate intensifications of absorbing heat fast, and not resembling the water bath in a large amount of heat energy injected waters, ELISA Plate only passive, absorb heat slowly, so saved the energy.
Description of drawings
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is that the quick couveuse of ELISA Plate and constant water bath box are hatched curve comparison figure.
Fig. 2 is the quick couveuse structural representation of ELISA Plate.
Among Fig. 1, solid line is the quick couveuse shop drawing of ELISA Plate, and dotted line is that constant water bath box is hatched shop drawing.Curve (1) is the quick couveuse camera incubata of ELISA Plate temperature variation line; Curve (2) is hatched process temperature for ELISA Plate and is changed line.Curve (3) is constant water bath box camera incubata constant temperature line; Curve (4) is that the incubation temperature of ELISA Plate in constant water bath box changes line.Be not difficult to find out that from figure ELISA Plate will be raised on the constant temperature line (being generally 37 degrees centigrade) that the best hatches from the normal temperature state, needs long temperature-rise period in constant water bath box.Evidence will reach the E point near constant temperature line, needs more than 50 minutes, and put the best section of hatching from E to F, only needs get final product in 5 minutes.This shows, the whole process of hatching, the overwhelming majority is wasted on the very long temperature-rise period.
And the quick couveuse of ELISA Plate is the camera incubata temperature to be raised to be higher than constant temperature point more than 4 degrees centigrade at short notice, widen the temperature difference of camera incubata and ELISA Plate, when curve (2) ELISA Plate temperature also differs from 1,2 degree centigrade from constant temperature point, curve (1) camera incubata temperature also was raised to peak B (this point was according to the actual conditions setting this moment, generally be higher than in constant temperature point 4-20 degree centigrade), then disconnect heating, fan blower continues the air draft fast cooling.The temperature-fall period temperature difference is still very large, and ELISA Plate continue to heat up, and when arrival during near the C point of constant temperature point, the camera incubata temperature also drops to rapidly constant temperature point constant temperature, ELISA Plate and hatch room temperature and hatch in the situation that the constant temperature point reaches unanimity.If after this also hatched 5 minutes, add camera incubata heat up drop to again constant temperature process less than 10 minutes, the time spent is less than 15 minutes altogether, water bath is hatched then and is needed about 1 hour.
In Fig. 2 structural representation, 5, chassis lid, 6, ELISA Plate depression bar, 7, ELISA Plate, 8, ELISA Plate pallet, 9, vibration motor, 10, eccentric wheel, 11, temperature sensor, 12, spring, 13, show and operation push-button 14, PTC heater, 15, fan, 16, exhaust fan, 17, with the circuit board of microcomputer, 18, cabinet, 19, bottom.
Embodiment
In demonstration and the upper man-machine conversation of operation push-button (13) the reaction incubation time is set, the colour developing incubation time needs the temperature value of constant temperature, based on the camera incubata lifting temperature number of constant temperature point, the parameters such as vibration motor (9) rotating speed are not just preserve as not revising forever after parameter arranges.Open chassis lid (5), the ELISA Plate (7) that adds reagent is placed on the ELISA Plate pallet (8), ELISA Plate depression bar (6) is mentioned and 90-degree rotation compression ELISA Plate, chassis lid closes.During work, start-up operation button (13), microcomputer (17) sends instruction, and two fans (15), the vibration motor (9) of two PTC heaters (14) and series connection thereof are worked simultaneously about making.The vibrations that the eccentric wheel that connects on the vibration motor axle (10) rotation produces drive whole pallet and ELISA Plate, and the liquid in the ELISA Plate is fully stirred.The heat energy that while PTC sends is under the fan of two inclinations and arranged in dislocation blows, and whole camera incubata forms the rotation hot blast and evenly toasts ELISA Plate.Along with the rapid rising of camera incubata temperature, ELISA Plate is also approached to the constant temperature point fast.When temperature sensor (11) detects predefined lifting temperature and counts, this moment, the ELISA Plate temperature also just in time approached the constant temperature point of setting, so stop PTC heater, starting simultaneously exhaust fan (16) discharges the heat of camera incubata fast, and change at the constant temperature point constant temperature of setting, make ELISA Plate in isoperibol, continue to hatch, until regularly finish, alarm is finished the whole process of hatching.
Claims (4)
1. quick couveuse of ELISA Plate, it is characterized in that adopting heating early stage and bring up a camera incubata hot environment, the temperature difference of ELISA Plate and camera incubata is increased and be rapidly heated, when constant temperature point that the temperature of ELISA Plate is hatched near the best, the temperature of camera incubata also drops to rapidly constant temperature point constant temperature.
2. the quick couveuse of ELISA Plate according to claim 1 is characterized in that the camera incubata hot environment is to add 4-20 degree centigrade on the basis of best incubation temperature.
3. the quick couveuse of ELISA Plate according to claim 1 is characterized in that best incubation temperature is 37 degrees centigrade.
4. the quick couveuse of ELISA Plate according to claim 1 is characterized in that the camera incubata hot environment of achievements of heating early stage, drops to rapidly the best again and hatches a constant temperature, and the used time was less than 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110289894.7A CN103018433B (en) | 2011-09-28 | 2011-09-28 | Rapid elisa plate incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110289894.7A CN103018433B (en) | 2011-09-28 | 2011-09-28 | Rapid elisa plate incubator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103018433A true CN103018433A (en) | 2013-04-03 |
| CN103018433B CN103018433B (en) | 2015-01-21 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110289894.7A Active CN103018433B (en) | 2011-09-28 | 2011-09-28 | Rapid elisa plate incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103018433B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108872596A (en) * | 2018-07-05 | 2018-11-23 | 重庆巴而思生物科技有限公司 | A kind of ELISA detection kit of HPV16 L1 antibody |
| CN117772296A (en) * | 2023-12-26 | 2024-03-29 | 东莞市欣宝仪器有限公司 | Constant temperature and humidity test box and control method thereof |
| CN117804056A (en) * | 2024-02-29 | 2024-04-02 | 成都千麦医学检验所有限公司 | Temperature-changing incubator for enzyme-linked immunosorbent assay |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002088295A1 (en) * | 2001-04-26 | 2002-11-07 | Societe Nouvelle Cell Tissue Progress | Thermally operated cell and tissue culture device |
| WO2008022658A1 (en) * | 2006-08-25 | 2008-02-28 | Universität Hannover | Process and apparatus for ion channel characterization |
| CN201454561U (en) * | 2009-06-30 | 2010-05-12 | 邱重任 | Multi-station separate heating insulation and timing display ELISA plate incubator |
| CN201637743U (en) * | 2010-02-09 | 2010-11-17 | 深圳市爱康电子有限公司 | ELIAS plate positioning, oscillating and incubating integral machine |
-
2011
- 2011-09-28 CN CN201110289894.7A patent/CN103018433B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002088295A1 (en) * | 2001-04-26 | 2002-11-07 | Societe Nouvelle Cell Tissue Progress | Thermally operated cell and tissue culture device |
| WO2008022658A1 (en) * | 2006-08-25 | 2008-02-28 | Universität Hannover | Process and apparatus for ion channel characterization |
| CN201454561U (en) * | 2009-06-30 | 2010-05-12 | 邱重任 | Multi-station separate heating insulation and timing display ELISA plate incubator |
| CN201637743U (en) * | 2010-02-09 | 2010-11-17 | 深圳市爱康电子有限公司 | ELIAS plate positioning, oscillating and incubating integral machine |
Non-Patent Citations (1)
| Title |
|---|
| 徐树良等: "分窗数字显时自动酶免孵育仪的应用", 《中国输血杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108872596A (en) * | 2018-07-05 | 2018-11-23 | 重庆巴而思生物科技有限公司 | A kind of ELISA detection kit of HPV16 L1 antibody |
| CN117772296A (en) * | 2023-12-26 | 2024-03-29 | 东莞市欣宝仪器有限公司 | Constant temperature and humidity test box and control method thereof |
| CN117804056A (en) * | 2024-02-29 | 2024-04-02 | 成都千麦医学检验所有限公司 | Temperature-changing incubator for enzyme-linked immunosorbent assay |
| CN117804056B (en) * | 2024-02-29 | 2024-05-14 | 成都千麦医学检验所有限公司 | Temperature-changing incubator for enzyme-linked immunosorbent assay |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103018433B (en) | 2015-01-21 |
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Owner name: CHENGDOU GAISEN ELECTRIC EQUIPMENT CO., LTD. Free format text: FORMER OWNER: WU YONG Effective date: 20150226 |
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Effective date of registration: 20150226 Address after: 610000 No. 138, Jin Sheng Road, Jinjiang District Industrial Park, Sichuan, Chengdu Patentee after: Chengdu an electronic equipment Co. Ltd. Address before: 610081 1-1-2102, No. 126, four street, Chenghua District, Sichuan, Chengdu Patentee before: Wu Yong |