CN103007346A - Anti-adhesion endocranium prepared by physical sedimentation method - Google Patents
Anti-adhesion endocranium prepared by physical sedimentation method Download PDFInfo
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- CN103007346A CN103007346A CN2012105895625A CN201210589562A CN103007346A CN 103007346 A CN103007346 A CN 103007346A CN 2012105895625 A CN2012105895625 A CN 2012105895625A CN 201210589562 A CN201210589562 A CN 201210589562A CN 103007346 A CN103007346 A CN 103007346A
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Abstract
The invention describes stent materials for clinical endocranium reconstitution and a preparation method thereof, belonging to the field of medical biomaterials and mainly solving the problems that clinically suture is needed for endocranium repair and endocranium is prone to adhesion at present. The aim of endocranium repair is achieved by providing a bioactive matrix which has a certain pore diameter and viscosity and adding anti-adhesion chitosan to one layer of the product for the endocranium receptor. The materials prepared by the method are I type collagens of different concentrations and intrinsic three-dimensional structures of the I type collagens and chitosan in different space, are uniform, have certain viscosity, reduce the surgical suture operation, adopts the chitosan anti-adhesion layer on the basis of maintaining the original activities of the I type collagens, reduce the probability of adhesion with cranium after surgery, have good biocompatibility and are beneficial to endocranium repair and reconstitution.
Description
Technical field
The present invention relates to a kind of chitosan and type i collagen mixes by certain proportion, form the structural remodeling of chitosan and type i collagen, then by physical method will not be combined with chitosan type i collagen separate, form the biomembrane material that the method for many chitosans and type i collagen layer and type i collagen layer makes.Be a kind of little Huang, softness, porous, preferably product of certain viscosity, biocompatibility arranged.Be used for the prosthetic dural substitutes of department of cerebral surgery cerebral dura mater.Belong to the biomaterial for medical purpose field.
Background technology
The replacement cerebral dura mateies such as the dural substitutes of at present domestic clinical use bovine pericardium commonly used, the bag that nourishes heart, Cor Sus domestica bag, cattle peritoneum, sheep peritoneum, mesentery carry out the cerebral dura mater reparation, but these materials all need to sew up, and the harder difficulty of materials and cerebral tissue surface attach fully, and easily cause adhesion.
In view of this, develop a kind of good biocompatibility; Certain viscosity is arranged, need not sew up during operation and can prevent that the biological cerebral dura mater of adhesion from becoming particularly important.Everybody generally acknowledges that collagen is the matrix components that many tissues exist, and is beneficial to the growth of various kinds of cell, reduced immunogenicity, good biocompatibility at present.Chitosan has antibacterial effect with preventing adhesion simultaneously.Therefore our company has adopted and has utilized type i collagen and chitosan to carry out by a certain percentage structural remodeling, form the chitosan of new structure and the mixture of type i collagen, then by physical method the type i collagen that does not carry out structural remodeling is separated, formed bilevel method and made the viscosity cerebral dura mater.One deck that this material carries out structural remodeling is the one deck that has viscosity and prevent adhesion, is the braincap osteoplaque.The type i collagen one deck that does not carry out structural remodeling be have viscosity can and the intracranial bone one deck of combining closely be the intracranial osteoplaque.And all have viscosity, can in operation process, reduce sew application.Reduced the infection probability.
Summary of the invention
This material selection a kind of chitosan and type i collagen mix by a certain percentage, the type i collagen that will not be combined with chitosan by physical method again separates, the method that forms chitosan and type i collagen layer and type i collagen layer makes.Can satisfy in the cerebral dura mater prothesis without operation stitching and the requirement that prevents tissue adhesion.Main operational steps is as follows:
1, the structural remodeling of chitosan and type i collagen: commercially available type i collagen and chitosan be (10～1) in mass ratio: (1～10) is dissolved in the acetum of 0.01mol/L～1.0mol/L, and type i collagen concentration is 0.1%～60.0% (w/w).
2, the separating treatment of structural remodeling type i collagen not: the mixed liquor that chitosan and type i collagen is carried out structural remodeling carries out plastic film mulch by required size, and the mould that then will complete film is placed on the automatic cradle, and automatic shaking separates 30min～300min.
3, the dural formation of viscosity: the biomembrane dehydration that separating treatment is good, making its water content is 1.0%～40.0%, packs irradiation sterilization.
The specific embodiment
Embodiment 1,
Get commercially available type i collagen 1.0g, commercially available chitosan 0.5g, being dissolved in 100ml concentration is in the 0.2mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 60min, with the dehydration of gained biomembrane, making its water content is 40.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 2: 1.
Embodiment 2,
Get commercially available type i collagen 10.0g, commercially available chitosan 3.0g, being dissolved in 100ml concentration is in the 0.05mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 120min, with the dehydration of gained biomembrane, making its water content is 10.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 10: 3.
Embodiment 3,
Get commercially available type i collagen 2.0g, commercially available chitosan 0.5g, being dissolved in 100ml concentration is in the 0.01mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 30min, with the dehydration of gained biomembrane, making its water content is 1.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 4: 1.
Embodiment 4,
Get commercially available type i collagen 50.0g, commercially available chitosan 10.0g, being dissolved in 100ml concentration is in the 1.0mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 300min, with the dehydration of gained biomembrane, making its water content is 40.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 5: 1.
Embodiment 5,
Get commercially available type i collagen 15.0g, commercially available chitosan 10.0g, being dissolved in 100ml concentration is in the 0.1mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 150min, with the dehydration of gained biomembrane, making its water content is 8.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 3: 2.
Embodiment 6,
Get commercially available type i collagen 0.2g, commercially available chitosan 0.1g, being dissolved in 100ml concentration is in the 0.01mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 30min, with the dehydration of gained biomembrane, making its water content is 5.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 2: 1.
Embodiment 7,
Get commercially available type i collagen 30.0g, commercially available chitosan 5.0g, being dissolved in 100ml concentration is in the 0.8mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 200min, with the dehydration of gained biomembrane, making its water content is 15.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 6: 1.
Embodiment 8,
Get commercially available type i collagen 40.0g, commercially available chitosan 5.0g, being dissolved in 100ml concentration is in the 0.08mol/L acetum.After the dissolving, pour mixed liquor into the mould pallet fully, be placed on the automatic cradle, automatic shaking 90min, with the dehydration of gained biomembrane, making its water content is 30.0% at last.Pack rear irradiation sterilization.Type i collagen: the mass ratio of chitosan is 8: 1.
Claims (1)
1. a chitosan and type i collagen are pressed certain mass than mixing, and the type i collagen that will not be combined with chitosan by physical method again separates, and form the double-deck biological cerebral dura mater that the method for chitosan and type i collagen layer and type i collagen layer makes.Be a kind of little Huang, softness, porous, preferably product of certain viscosity, biocompatibility arranged.Wherein chitosan and type i collagen layer are antiblocking layers.Can satisfy in the cerebral dura mater prothesis without operation stitching and the requirement that prevents tissue adhesion.Its feature comprises the following steps:
(1), with commercially available type i collagen and chitosan in mass ratio (10～1): (1～10) is dissolved in the acetum of 0.01mol/L～1.0mol/L, and type i collagen concentration is 0.1%～60.0% (w/w).Finish the structural remodeling of chitosan and type i collagen.
(2), mixed liquor that chitosan and type i collagen are carried out structural remodeling carries out plastic film mulch by required size, the mould that then will complete film is placed on the automatic cradle, automatic shaking separates 30min～300min.The type i collagen that to not be combined with chitosan according to the difference of molecular weight and molecular structure separates.
(3), the dehydration of biomembrane that separating treatment is good, making its water content is 1.0%～40.0%, packs irradiation sterilization.Formation has the biological cerebral dura mater of viscosity of double-deck material.
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Cited By (5)
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| CN103263694A (en) * | 2013-05-14 | 2013-08-28 | 北京华信佳音医疗科技发展有限责任公司 | Collagen-based dura and preparation method thereof |
| CN104888273A (en) * | 2015-05-14 | 2015-09-09 | 四川大学 | Double-layer composite cerebral dura mater, and preparation method thereof |
| CN106474563A (en) * | 2016-12-02 | 2017-03-08 | 上海其胜生物制剂有限公司 | A kind of method that reversal temperature sensitive technology prepares artificial dura mater |
| CN106512065A (en) * | 2016-10-20 | 2017-03-22 | 天津卫凯生物工程有限公司 | Three-dimensional scaffold applied to cell culture and preparation method thereof |
| CN112915256A (en) * | 2021-01-11 | 2021-06-08 | 华南理工大学 | Method for preparing human dura mater repair material with imitated ECM (extracellular matrix) components and structure by long fiber network framework preforming method |
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| US20030232198A1 (en) * | 2002-02-21 | 2003-12-18 | Encelle, Inc. | Immobilized bioactive hydrogel matrices as surface coatings |
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| CN103263694A (en) * | 2013-05-14 | 2013-08-28 | 北京华信佳音医疗科技发展有限责任公司 | Collagen-based dura and preparation method thereof |
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| CN106512065A (en) * | 2016-10-20 | 2017-03-22 | 天津卫凯生物工程有限公司 | Three-dimensional scaffold applied to cell culture and preparation method thereof |
| CN106474563A (en) * | 2016-12-02 | 2017-03-08 | 上海其胜生物制剂有限公司 | A kind of method that reversal temperature sensitive technology prepares artificial dura mater |
| CN106474563B (en) * | 2016-12-02 | 2019-04-16 | 上海其胜生物制剂有限公司 | A kind of method of reversal temperature sensitive technology preparation artificial dura mater |
| CN112915256A (en) * | 2021-01-11 | 2021-06-08 | 华南理工大学 | Method for preparing human dura mater repair material with imitated ECM (extracellular matrix) components and structure by long fiber network framework preforming method |
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Application publication date: 20130403 |