CN103005219A - Sow feed and preparation method thereof - Google Patents
Sow feed and preparation method thereof Download PDFInfo
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- CN103005219A CN103005219A CN2012105887173A CN201210588717A CN103005219A CN 103005219 A CN103005219 A CN 103005219A CN 2012105887173 A CN2012105887173 A CN 2012105887173A CN 201210588717 A CN201210588717 A CN 201210588717A CN 103005219 A CN103005219 A CN 103005219A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 150000004676 glycans Chemical class 0.000 claims abstract description 43
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 43
- 239000005017 polysaccharide Substances 0.000 claims abstract description 43
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000006228 supernatant Substances 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000000047 product Substances 0.000 claims abstract description 24
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 22
- 108090000790 Enzymes Proteins 0.000 claims abstract description 22
- 239000002244 precipitate Substances 0.000 claims abstract description 21
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 12
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- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 8
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- 238000004108 freeze drying Methods 0.000 claims abstract description 3
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- 241000195493 Cryptophyta Species 0.000 claims description 23
- 229940088598 enzyme Drugs 0.000 claims description 21
- 238000001556 precipitation Methods 0.000 claims description 14
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 9
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- 108091005804 Peptidases Proteins 0.000 claims description 7
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 7
- 240000002900 Arthrospira platensis Species 0.000 claims description 6
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 6
- 229940082787 spirulina Drugs 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000011573 trace mineral Substances 0.000 claims description 5
- 235000013619 trace mineral Nutrition 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
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- 230000001186 cumulative effect Effects 0.000 claims description 4
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- 238000012545 processing Methods 0.000 claims description 4
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- KIPLYOUQVMMOHB-MXWBXKMOSA-L [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O Chemical compound [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O KIPLYOUQVMMOHB-MXWBXKMOSA-L 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 235000019419 proteases Nutrition 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 229940063650 terramycin Drugs 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
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- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000009413 insulation Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 abstract description 4
- 235000019764 Soybean Meal Nutrition 0.000 abstract 1
- 241001052560 Thallis Species 0.000 abstract 1
- 230000003544 deproteinization Effects 0.000 abstract 1
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- 239000004455 soybean meal Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 20
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 12
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- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
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- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 description 3
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Landscapes
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a sow feed and a preparation method thereof. The preparation method comprises the following steps of: 1. carrying out hot water and centrifugal treatment on collected wet microalgae thalli in sequence to obtain supernatant A and a precipitate A; 2. carrying out enzyme addition and centrifugal treatment on the precipitate A in sequence to obtain supernatant B and a precipitate B; 3. mixing the supernatant A with the supernatant B and then concentrating the mixture, adding cold ethanol at 4 DEG C and standing for more than 12 hours and filtering the mixed liquor to obtain a crude polysaccharide precipitate; 4. adding water to dissolve the crude polysaccharide precipitate obtained in the step 3, carrying out deproteinization treatment on the crude polysaccharide precipitate to separate the crude polysaccharide precipitate into a protein precipitate and deproteinized polysaccharide solution and freeze-drying or drying the protein precipitate to obtain a crude protein product; and 5. separating an intermediate thallus containing a high protein product from the precipitate B and mixing the following components in parts by weight to prepare the sow feed: 5-10 parts of obtained crude protein product and/or 5-10 parts of dried high protein product of the intermediate thallus, 50-75 parts of corn, 6-10 parts of bran, 5-22 parts of soybean meal, 0.01-0.03 part of synbiotics and 0.01-0.03 part of oligosaccharide.
Description
Technical field
The present invention relates to a kind of sow feed and preparation method thereof.
Background technology
Along with prevailing of scientific cultivation, people have had more deep awareness and understanding for the cultivation of domestic animal, how to improve the survival rate of domestic animal, reduce the generation of disease, and accelerate its growth rate and improve meat quality, be the urgent inquisitive problems of numerous cultivation dealers.
Existing sow feed all is to come from cereal crops, does not use algae as raw material, has caused a large amount of foodstuff wastes, is unfavorable for the demand of human survival.
In addition, the different growth phases of pig are also different to the demand of nutritional labeling, the pig of period of pregnancy particularly, and traditional method for breeding but not for the pig of period of pregnancy, is implemented the feed of Different Nutrition component content, thereby has been affected growing of pig period of pregnancy.
Summary of the invention
The present invention has designed a kind of sow feed and preparation method thereof, and the technical problem of its solution is: (1) need to use a large amount of dregs of beans and corn in order to guarantee the protein content in the existing sow feed, and is difficult for fully being absorbed by sow; (2) existing sow feed all is to come from cereal crops, does not come from algae, has caused a large amount of foodstuff wastes, is unfavorable for the demand of human survival; (3) traditional method for breeding but not for the pig of period of pregnancy, is implemented the feed of Different Nutrition component content, thereby has been affected growing of pig period of pregnancy.
In order to solve the technical problem of above-mentioned existence, the present invention has adopted following scheme:
A kind of sow feed preparation method may further comprise the steps:
Step 1: little algae or little algae wet thallus are passed through hot water treatment and centrifugal treating successively, obtain supernatant A and precipitate A; Wherein, the mass ratio of hot water and little algae or little algae wet thallus is 20-30:1;
Step 2: precipitate A is passed through enzyme-added processing and centrifugal treating successively, obtains supernatant B and deposit B; Wherein, the mass ratio of precipitate A and enzyme is 40-100:1.
Step 3: will supernatant A and supernatant B concentrate after mixing, and add 4 ℃ of cold ethanol and mix more than 12 hours, mixed liquor is filtered obtains thick polysaccharide precipitation; Wherein, the cumulative volume of supernatant A and supernatant B is concentrated to 1/5-1/4 of original volume, and the volume that adds cold ethanol is concentrated rear supernatant A and supernatant B cumulative volume 3-5 times.
Step 4: the thick polysaccharide precipitation of gained is dissolved in water in the step 3, takes off albumen and processes, and is separated into albumen precipitation and Deproteinated polysaccharide solution, and albumen precipitation obtains the crude protein product by freeze drying or oven dry; Taking off polysaccharide solution behind the albumen can decolour and obtain refining polysaccharide.
Step 5: the bacteria suspension of gained deposit B in the step 2 is regulated after the pH value is 10-12,70-80 ℃ of insulation 1-2h, then add the sodium chloride solution of 0.8% concentration and the mixed liquor of n-hexane and ethanol, finally make n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min leaves standstill and is divided into three layers and is followed successively by from top to bottom: n-hexane phase, middle thalline phase and pure water; N-hexane can obtain uranidin mutually.
Step 6: be mixed and made into sow feed according to following mass ratio: thalline forms after super-dry in the middle of the crude protein product that 5-10 part step 4 obtains and/or the 5-10 part step 5 high protein product, 50-75 part corn, 6-10 part wheat bran, 5-22 part dregs of beans, 0.01-0.03 part Synbiotics and 0.01-0.03 part oligosaccharide.
Further, little algae is for being chlorella, spirulina or grid algae; Little algae wet thallus is chlorella thalline, spirulina thalline or grid phycomycete body.
Further, the hot water temperature in the step 1 and processing time are respectively 90-100 ℃ and 0.5-2h.
Further, the enzyme in the step 2 is selected from one or more combinations in neutral proteinase, alkali protease and the cellulase; Cellulose enzyme activity is 1200-1500U/g, and the neutral proteinase enzyme is lived and is 59000-60000U/g, and the work of alkali protease enzyme is 2 * 105-2.02 * 105U/g.
Further, in the sow feed of the final gained of step 6, also add trace element.
Further, the mass fraction of trace element is according to following ratio: 0.4-0.6 portion of terramycin, 0.2-1.5 part copper sulphate, 0.2-1.5 part ferrous sulfate, 0.3-1.8 part zinc sulfate and 1-2 portions of salt.
This invention also comprises by above-mentioned 6 kinds of sow feeds that the preparation method makes.
This sow feed and preparation method thereof is compared with traditional sow feed and preparation method thereof, has following beneficial effect:
(1) the present invention is by isolating crude protein product and high protein product from algae, so that the protein content in the sow feed is higher than conventional feed, raising for sow provides enough protein, the simultaneously source of this protein and algae, thereby can reduce corn and dregs of beans use amount.
(2) the present invention designs and uses hot water treatment as preprocessing means, increases the cell permeability, suitably destroys cell wall structure, reduces the use amount of enzyme process enzyme process action time and enzyme, by hot-water extraction and enzymolysis, obtains product polysaccharide and a small amount of crude protein.
(3) the present invention is except producing sow feed, and can decolour in the polysaccharide solution behind its by-product pint albumen obtains refining polysaccharide.
(4) the present invention is except producing sow feed, and its byproduct n-hexane can obtain uranidin in mutually.
(5) increase trace element among the present invention and can satisfy pig growth and development to the needs of various mineral matter elements, especially can make the piggy ramp.
(6) the present invention add Synbiotics can strengthen body immunity suppress the harmful bacteria breeding, substitute the prevention swinery disease of adding in the feed antibiotic, improve efficiency of feed utilization and make the dry odorless of sow ight soil, fly reduce, reduce the respiratory tract incidence of disease.
(7) the present invention adds increment that oligosaccharide can optionally promote profitable strain in the sow enteron aisle, the definite value that stops pathogen in the enteron aisle promotes it at will to excrete, stimulate and strengthen the immune response of body and improve body to the absorption of the mineral matter in the food.
The specific embodiment
In conjunction with the following example, the present invention will be further described:
Embodiment 1: select chlorella.
With the centrifugal 10min of 4000rpm/min, the removal supernatant obtains the chlorella wet thallus, takes by weighing chlorella wet thallus 1.0g in the 100ml conical flask, adds the 25ml deionized water with the chlorella zymotic fluid, 90 ℃ of heating 1.5h, cooling, centrifugal supernatant A and the precipitate A of getting.Take by weighing the 0.065g cellulase, 0.035g neutral proteinase, cellulose enzyme activity are 1200U/g, and the neutral proteinase enzyme is lived and to be 60000U/g, adds the citric acid-sodium citrate buffer of pH=5, and pH=5 is the pH reaction system of optimum enzyme effect selected in the experiment.Be settled to 100ml, get the 25ml enzyme solutions and join in the chlorella wet thallus precipitate A, hydrolysis temperature is 42 ℃, stirs 4h, 90 ℃ of enzyme 10min that go out.Suspension after the hydrolysis is carried out centrifugal 10min under the 4000r/min, obtain supernatant B and deposit B.
Merge supernatant A and supernatant B, concentrated this supernatant mixed liquor is to 1/5 of original volume, and the 4 ℃ of concussions of cold ethanol that add 3 times of volumes mix, and leave standstill 12h, filters to get thick polysaccharide precipitation.
Get the thick polysaccharide precipitation of the water-soluble solution of 10ml, regulate pH to 3 with trichloroacetic acid, mixing leaves standstill 2h, adds the n-butanol of 15 times of trichloroacetic acid volumes, stirs, and leaves standstill 1h, and is centrifugal, and the aqueous solution reclaims polysaccharide, repeats 2 times.Polysaccharide extract rate 82.3%.Water dissolving polysaccharide carries out adsorption bleaching with the flow velocity of 2BV/h to the thick polysaccharide solution of 2.5mg/ml, records pigment removal efficiency 92.4%, and the polysaccharide retention rate is 88.97%.Protein solubility is lower and polysaccharide loss is less during pH=3, is the peak optimization reaction system.The use of n-butanol also is in order to reduce the water-soluble of protein, thus crude protein product and separation of polysaccharides.Use separately the TCA method also passable herein, increase the number of times that repeats to extract.
It is 11 that the bacteria suspension of deposit B is regulated pH with the NaOH of 2mol/L, 70 ℃ of reaction 60min, cooling, adding an amount of 0.8% sodium chloride solution and the mixed liquor n-hexane of n-hexane and ethanol and the volume ratio of ethanol is 2:4, and make final n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, collect the n-hexane phase, the n-hexane that adds again initial 1/3 volume repeats twice, and n-hexane phase A adds anhydrous sodium sulfate and removes minor amount of water, reclaims solvent and obtains the uranidin such as carrotene.Thalline its crude protein content of high protein product after drying that the intermediate layer suspends is 38.7%, and 18 water solution amino acid content is abundant, can be used as be rich in albumen fully, amino acid whose composition is used as the part substitute that protein feed becomes sow meals feed.
Choose 5 parts of crude protein products, 10 parts of high protein products, 50 parts of corns, 8 parts of wheat brans, 15 parts of dregs of beans, 0.01 part of Synbiotics and 0.01 part of oligosaccharide.So that they fully mix, can become the sow feed of high-quality by agitating device.
Embodiment 2: select the grid algae.
With the centrifugal 10min of 4000rpm/min, the removal supernatant obtains grid phycomycete body, takes by weighing grid algae wet thallus 1.0g in the 100ml conical flask, adds the 25ml deionized water with grid algae zymotic fluid, 90 ℃ of heating 1.5h, cooling, centrifugal supernatant A and the precipitate A of getting.Take by weighing the 0.075g cellulase, 0.025g alkali protease, cellulose enzyme activity is 1200U/g, and the work of alkali protease enzyme is 2 * 105U/g, and the citric acid-sodium citrate buffer that adds pH=4 is settled to 100ml, getting the 25ml enzyme solutions joins in the grid algae wet thallus precipitate A, hydrolysis temperature is 40 ℃, stirs 4h, 90 ℃ of enzyme 10min that go out, suspension after the hydrolysis is carried out centrifugal 10min under the 4000r/min, obtain supernatant B and deposit B.
Merge supernatant A and supernatant B, concentrated this supernatant mixed liquor is to 1/5 of original volume, and the 4 ℃ of concussions of cold ethanol that add 3 times of volumes mix, and leave standstill 12h, filters to get thick polysaccharide precipitation.
Get the thick polysaccharide precipitation of the water-soluble solution of 10ml, regulate pH to 3 with trichloroacetic acid, mixing leaves standstill 2h, adds the n-butanol of 15 times of trichloroacetic acid volumes, stirs, and leaves standstill 1h, and is centrifugal, and the aqueous solution reclaims polysaccharide, repeats 2 times.Polysaccharide extract rate 82.3%.Water dissolving polysaccharide carries out adsorption bleaching with the flow velocity of 2BV/h to the thick polysaccharide solution of 2.5mg/ml, records pigment removal efficiency 92.4%, and the polysaccharide retention rate is 88.97%.Protein solubility is lower and polysaccharide loss is less during pH=3, is the peak optimization reaction system.The use of n-butanol also is in order to reduce the water-soluble of protein, thus crude protein product and separation of polysaccharides.Use separately the TCA method also passable herein, increase the number of times that repeats to extract.
It is 11 that the bacteria suspension of deposit B is regulated pH with the NaOH of 2mol/L, 70 ℃ of reaction 60min, cooling, adding an amount of 0.8% sodium chloride solution and the mixed liquor n-hexane of n-hexane and ethanol and the volume ratio of ethanol is 2:4, and make final n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, collect the n-hexane phase, the n-hexane that adds again initial 1/3 volume repeats twice, and n-hexane phase A adds anhydrous sodium sulfate and removes minor amount of water, reclaims solvent and obtains the uranidin such as carrotene.Thalline its crude protein content of high protein product after drying that the intermediate layer suspends is 38.7%, and 18 water solution amino acid content is abundant, can be used as be rich in albumen fully, amino acid whose composition is used as the part substitute that protein feed becomes sow meals feed.
Choose 6 parts of crude protein products, 10 parts of high protein products, 60 parts of corns, 6 parts of wheat brans, 20 parts of dregs of beans, 0.01 part of Synbiotics and 0.01 part of oligosaccharide, so that they fully mix, can become the sow feed of high-quality by agitating device.
Embodiment 3: select spirulina.
With the centrifugal 10min of 4000rpm/min, the removal supernatant obtains the spiral thalline, takes by weighing spiral wet thallus 0.8g in the 100ml conical flask, adds the 25ml deionized water with the spirulina zymotic fluid, 90 ℃ of heating 1.5h, cooling, centrifugal supernatant A and the precipitate A of getting.Take by weighing the 0.067g neutral proteinase, the alkali protease enzyme is lived and is 60000U/g, and the citric acid-sodium citrate buffer that adds pH=7 is settled to 100ml, gets the 20ml enzyme solutions to join in the grid algae wet thallus, and hydrolysis temperature is 42 ℃, stirs 6h, 90 ℃ of enzyme 10min that go out.Suspension after the hydrolysis is carried out centrifugal 10min under the 4000r/min, obtain supernatant B and deposit B.
Merge supernatant A and supernatant B, concentrated this supernatant mixed liquor is to 1/5 of original volume, and the 4 ℃ of concussions of cold ethanol that add 3 times of volumes mix, and leave standstill 12h, filters to get thick polysaccharide precipitation.
Get the thick polysaccharide precipitation of the water-soluble solution of 10ml, regulate pH to 3 with trichloroacetic acid, mixing leaves standstill 2h, adds the n-butanol of 15 times of trichloroacetic acid volumes, stirs, and leaves standstill 1h, and is centrifugal, and the aqueous solution reclaims polysaccharide, repeats 2 times.Polysaccharide extract rate 82.3%.Water dissolving polysaccharide carries out adsorption bleaching with the flow velocity of 2BV/h to the thick polysaccharide solution of 2.5mg/ml, records pigment removal efficiency 92.4%, and the polysaccharide retention rate is 88.97%.Protein solubility is lower and polysaccharide loss is less during pH=3, is the peak optimization reaction system.The use of n-butanol also is in order to reduce the water-soluble of protein, thus crude protein product and separation of polysaccharides.Use separately the TCA method also passable herein, increase the number of times that repeats to extract.
It is 11 that the bacteria suspension of deposit B is regulated pH with the NaOH of 2mol/L, 70 ℃ of reaction 60min, cooling, adding an amount of 0.8% sodium chloride solution and the mixed liquor n-hexane of n-hexane and ethanol and the volume ratio of ethanol is 2:4, and make final n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, collect the n-hexane phase, the n-hexane that adds again initial 1/3 volume repeats twice, and n-hexane phase A adds anhydrous sodium sulfate and removes minor amount of water, reclaims solvent and obtains the uranidin such as carrotene.Thalline its crude protein content of high protein product after drying that the intermediate layer suspends is 38.7%, and 18 water solution amino acid content is abundant, can be used as be rich in albumen fully, amino acid whose composition is used as the part substitute that protein feed becomes sow meals feed.
Choose 10 parts of high protein products, 65 parts of corns, 6 parts of wheat brans, 20 parts of dregs of beans, 0.02 part of Synbiotics and 0.01 part of oligosaccharide, 0.6 part of terramycin, 0.2 part of copper sulphate, 1 part of ferrous sulfate, 1.8 parts of zinc sulfate and 2 portions of salt.So that they fully mix, can become the sow feed of high-quality by agitating device.
The above has carried out exemplary description to the present invention in conjunction with the embodiments; obvious realization of the present invention is not subjected to the restriction of aforesaid way; as long as the various improvement of having adopted method design of the present invention and technical scheme to carry out; or without improving design of the present invention and technical scheme are directly applied to other occasion, all in protection scope of the present invention.
Claims (7)
1. sow feed preparation method may further comprise the steps:
Step 1: little algae or little algae wet thallus are passed through hot water treatment and centrifugal treating successively, obtain supernatant A and precipitate A; Wherein, the mass ratio of hot water and little algae or little algae wet thallus is 20-30:1;
Step 2: precipitate A is passed through enzyme-added processing and centrifugal treating successively, obtains supernatant B and deposit B; Wherein, the mass ratio of precipitate A and enzyme is 40-100:1;
Step 3: will supernatant A and supernatant B concentrate after mixing, and add 4 ℃ of cold ethanol and mix more than 12 hours, mixed liquor is filtered obtains thick polysaccharide precipitation; Wherein, the cumulative volume of supernatant A and supernatant B is concentrated to 1/5-1/4 of original volume, and the volume that adds cold ethanol is concentrated rear supernatant A and supernatant B cumulative volume 3-5 times;
Step 4: the thick polysaccharide precipitation of gained is dissolved in water in the step 3, takes off albumen and processes, and is separated into albumen precipitation and Deproteinated polysaccharide solution, and albumen precipitation obtains the crude protein product by freeze drying or oven dry;
Step 5: the bacteria suspension of gained deposit B in the step 2 is regulated after the pH value is 10-12,70-80 ℃ of insulation 1-2h, then add the sodium chloride solution of 0.8% concentration and the mixed liquor of n-hexane and ethanol, finally make n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min leaves standstill and is divided into three layers and is followed successively by from top to bottom: n-hexane phase A, middle thalline phase and pure water;
Step 6: be mixed and made into sow feed according to following mass ratio: thalline forms after super-dry in the middle of the crude protein product that 5-10 part step 4 obtains and/or the 5-10 part step 5 high protein product, 50-75 part corn, 6-10 part wheat bran, 5-22 part dregs of beans, 0.01-0.03 part Synbiotics and 0.01-0.03 part oligosaccharide.
2. described sow feed preparation method according to claim 1, it is characterized in that: little algae is for being chlorella, spirulina or grid algae; Little algae wet thallus is chlorella thalline, spirulina thalline or grid phycomycete body.
3. described sow feed preparation method according to claim 1, it is characterized in that: the hot water temperature in the step 1 and processing time are respectively 90-100 ℃ and 0.5-2h.
4. described sow feed preparation method according to claim 1, it is characterized in that: the enzyme in the step 2 is selected from one or more combinations in neutral proteinase, alkali protease and the cellulase; Cellulose enzyme activity is 1200-1500U/g, and the neutral proteinase enzyme is lived and is 59000-60000U/g, and the work of alkali protease enzyme is 2 * 105-2.02 * 105U/g.
5. any one described sow feed preparation method in 4 according to claim 1 is characterized in that: also add trace element in the sow feed of the final gained of step 6.
6. any one described sow feed preparation method in 5 according to claim 1 is characterized in that: the mass fraction of trace element is according to following ratio: 0.4-0.6 portion of terramycin, 0.2-1.5 part copper sulphate, 0.2-1.5 part ferrous sulfate, 0.3-1.8 part zinc sulfate and 1-2 portions of salt.
7. sow feed that makes to 6 any one preparation method according to claim 1.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210588717.3A CN103005219B (en) | 2012-12-31 | 2012-12-31 | Sow feed and preparation method thereof |
| CN201410376253.9A CN104187075B (en) | 2012-12-31 | 2012-12-31 | A kind of sow feed and preparation method thereof |
| CN201410376252.4A CN104171680B (en) | 2012-12-31 | 2012-12-31 | A kind of sow feed and preparation method thereof |
| CN201410376251.XA CN104171679B (en) | 2012-12-31 | 2012-12-31 | A kind of sow feed and preparation method thereof |
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| CN201410376252.4A Division CN104171680B (en) | 2012-12-31 | 2012-12-31 | A kind of sow feed and preparation method thereof |
| CN201410376251.XA Division CN104171679B (en) | 2012-12-31 | 2012-12-31 | A kind of sow feed and preparation method thereof |
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| CN201410376253.9A Expired - Fee Related CN104187075B (en) | 2012-12-31 | 2012-12-31 | A kind of sow feed and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104982731B (en) * | 2015-07-06 | 2017-12-19 | 中国农业科学院农业环境与可持续发展研究所 | A kind of method of waste water microalgae processing feed |
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| ES2611795B1 (en) * | 2015-11-06 | 2018-02-20 | Beatriz JIMÉNEZ ADANEZ | Product for animal feed based on germinated seeds and microalgae and manufacturing procedure |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN104171680A (en) | 2014-12-03 |
| CN104171679B (en) | 2016-03-09 |
| CN104187075B (en) | 2016-02-10 |
| CN103005219B (en) | 2014-07-16 |
| CN104171679A (en) | 2014-12-03 |
| CN104171680B (en) | 2016-02-10 |
| CN104187075A (en) | 2014-12-10 |
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