CN103005155A - Preparation method for solid powder dog-food flavoring agent - Google Patents
Preparation method for solid powder dog-food flavoring agent Download PDFInfo
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- CN103005155A CN103005155A CN2012105021221A CN201210502122A CN103005155A CN 103005155 A CN103005155 A CN 103005155A CN 2012105021221 A CN2012105021221 A CN 2012105021221A CN 201210502122 A CN201210502122 A CN 201210502122A CN 103005155 A CN103005155 A CN 103005155A
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Abstract
The invention discloses a preparation method for solid powder dog-food flavoring agent, belonging to the technical field of feeding process. The preparation method provided by the invention comprises the following steps of: using chicken breast meat as the main material; using protease to hydrolyze the main material; tracking and determining the degree of hydrolysis of enzymatic hydrolysate; obtaining enzymatic hydrolysate which is rich in amino acid, short-peptide and free of bitter taste; adding D-xylose, L-cysteine hydrochloride and vitamin B1 to the enzymatic hydrolysate for further reacting; adding tara gum and modified starch to mix and homogenize; and finally, spraying and drying to obtain the solid powder dog-food flavoring agent. The solid powder dog-food flavoring agent prepared by the preparation method provided by the invention has strong meat flavor and outstanding delicious taste, improves the utilization rate and additional value of the fresh chicken breast meat, and can cover the bad odor in the materials and the production process by re-dissolving and adding to the dog food. The solid powder is obtained by spraying and drying after being embedded by the tara gum and modified starch, so that the loss of the flavor components in the spraying and drying process is reduced. Compared with the market liquid dog-food flavoring agent, the solid powder dog-food flavoring agent prepared by the preparation method provided by the invention is more convenient to transport and store, and has good market prospect.
Description
Technical field
A kind of solid state powder shape of the present invention dog grain preparation of flavours method is specifically related to a kind of dog grain local flavor that improves, and improves the solid-state flavouring agent of its palatability, belongs to the feed processing technology field.
Background technology
Along with the national life level progressively improves, people also can more and more pay attention to diet and the health of pet, commercially available pet dog food replacing family self-control dog grain, become the selection of more families.Common pet dog food on the market is that the raw material that will contain the multiple nutrients material mixes mostly now, through the expanded dog grain of dry type expanded or extrusion molding.This type of dog grain causes product special flavour not good owing to being subject to the impact of raw material local flavor and extruding or bulking process, and bad smell is clearly arranged, and has affected the selective and feed intake of pet dog to dog grain.At present, internal feed is widely used in pig feed and chicken feed with flavouring agent, and the application study in the pet dog feed seldom, and the product on the market still less.Simultaneously, commercially available dog grain flavouring agent great majority are liquid form product, and solid product is very rare.Therefore, the invention provides a kind of solid state powder shape dog grain preparation of flavours method.Mainly take Fresh Grade Breast as primary raw material, with papain or flavor protease it is carried out enzymatic hydrolysis, degree of hydrolysis by the tracking and measuring enzymolysis liquid, effective controlled enzymatic hydrolysis time, obtain being rich in amino acid and small peptide and without the enzymolysis liquid of bitter taste, in the gained enzymolysis liquid, add D-wood sugar, Cys hydrochloride and Cobastab
1Deng composition, be applicable to further Maillard reaction and then produce strong meat fragrance and delicate flavour, after adding a certain proportion of tara gum and converted starch again and mixing homogeneous with it, last spray-drying makes solid state powder shape dog grain flavouring agent.
Summary of the invention
The object of the invention provides a kind of solid state powder shape dog grain preparation of flavours method.Mainly take Fresh Grade Breast as primary raw material, with papain or flavor protease it is hydrolyzed, degree of hydrolysis by the tracking and measuring enzymolysis liquid, effective controlled enzymatic hydrolysis time, obtain being rich in amino acid and small peptide and without the enzymolysis liquid of bitter taste, in the gained enzymolysis liquid, add D-wood sugar, Cys hydrochloride and Cobastab
1Deng composition, be applicable to further Maillard reaction and then produce strong meat fragrance and delicate flavour, after adding tara gum and converted starch again and mixing homogeneous with it, last spray-drying makes solid state powder shape dog grain flavouring agent.
The technical solution used in the present invention: a kind of solid state powder shape dog grain preparation of flavours method, step is:
(1) degrease freshly-slaughtered poultry brisket is twisted into meat gruel;
(2) in the meat gruel that step (1) obtains, add water by weight 1:2～1:4, fully stir, get the meat slurry;
(3) the meat slurry that step (2) is obtained is warming up to 40 ℃～60 ℃, regulates pH to 5.0～7.0, adds the protease of incarnation slurry quality 1.0% ~ 3.0 %;
Described protease is papain or flavor protease;
(4) enzymolysis: the meat that step (3) obtains is starched at 40 ℃～60 ℃ lower mechanical agitation enzymolysis time 4～6 h, followed the tracks of and survey degree of hydrolysis to 28%～42%, then be warming up to rapidly 85 ℃～90 ℃, continue to stir 20 min, get enzymolysis liquid;
(5) in the enzymolysis liquid that step (4) obtains, add enzymolysis liquid quality meter 0.3%～5% chicken fat, 2%～5% reduced sugar, 0.5%～5% Cys hydrochloride, 0～2% Cobastab
1, 0.5%～1% food grade quality concentration 85% phosphoric acid, stir;
(6) product that step (5) is obtained is at 110 ℃～120 ℃ lower continuous heating 40～50 min;
(7) product that step (6) is obtained adds the tara gum of products therefrom quality meter 2%～5%, 10%～25% converted starch, stirs;
(8) spray-drying behind the product homogeneous that step (7) is obtained, condition: EAT is 180 ℃～190 ℃, and leaving air temp is 80 ℃～90 ℃, and feed rate is 10 mL/min～15 mL/min, gets product solid state powder shape dog grain flavouring agent.
The degree of hydrolysis of step (4) tracking and measuring enzymolysis liquid is followed the tracks of and is surveyed degree of hydrolysis to 28%～32%, and the strict controlled enzymatic hydrolysis time is 4 ~ 6 h.
The described reduced sugar of step (5) is the D-wood sugar.
Application with the solid state powder shape dog grain flavouring agent of described method preparation: make an addition in the dog grain after it is redissolved by mass concentration 30%～40%, can hide dog grain raw material itself intrinsic with and the bad smell that produced in process of production, the suitableeest addition is to account for 0.2% of dog grain quality.
Beneficial effect of the present invention:
1, take Fresh Grade Breast as primary raw material, product cost is low, Effective Raise utilization rate and the added value of freshly-slaughtered poultry brisket.
2, with papain or flavor protease Fresh Grade Breast is hydrolyzed, by tracking and measuring enzymolysis liquid degree of hydrolysis, effective controlled enzymatic hydrolysis time, obtains being rich in amino acid and small peptide and without the enzymolysis liquid of bitter taste.
3, in the gained enzymolysis liquid, add D-wood sugar, Cys hydrochloride and Cobastab
1Deng composition, be applicable to further Maillard reaction and then produce strong meat fragrance and delicate flavour.
4, be spray dried to again solid state powder after utilizing tara gum and converted starch to its embedding, can reduce the loss of flavor components in spray-drying process, make it to be able to effective reservation.
5, the solid state powder shape dog grain flavouring agent meat aromatic flavour for preparing by the present invention, delicate flavour is outstanding, after making an addition to again dog grain after it is redissolved by concentration 30%～40%, can effectively hide dog grain raw material itself intrinsic with and the bad smell that produced in process of production, improve the palatability of dog grain, can satisfying the market on to the demand of fragrance type pet dog food.
6, compare with commercially available liquid dog grain flavouring agent, the solid state powder shape dog grain flavouring agent of the present invention's preparation will more be convenient to transportation and preserve, and in storage, avoid volatilization and the oxidation of flavouring agent flavor composition, can better keep its local flavor, be applied to and have good market prospects in the pet food.
Description of drawings
Fig. 1 dog grain flavouring agent searching for food in the self-control basal diet compared experimental result.
Fig. 2 dog grain flavouring agent becomes searching for food in the dog grain to compare experimental result at commercially available boccaccio.
The specific embodiment
(1) degrease freshly-slaughtered poultry brisket is twisted into meat gruel;
(2) in the meat gruel that step (1) obtains, add water by weight 1:3, fully stir;
(3) the meat slurry that step (2) is obtained is warming up to 45 ℃, regulates pH to 6.0～7.0, adds 2% papain;
(4) meat slurry mechanical agitation 6 h under 45 ℃ of conditions that step (3) obtained, controlled hydrolysis degree to 28%～32%; Then be warming up to rapidly 85 ℃～90 ℃, continue to stir 20 min;
(5) enzymolysis product that step (4) is obtained adds 1.0% chicken fat, 4.0% D-wood sugar, 1.0% Cys hydrochloride, 0.5% Cobastab
1, 1.0% food grade quality concentration 85% phosphoric acid, stir;
(6) product that step (5) is obtained is at 115 ℃ of lower continuous heating 50 min;
(7) product that step (6) is obtained stirs after adding 5% tara gum, 25% converted starch;
(8) carry out spray-drying, condition behind the product homogeneous that step (7) is obtained: EAT is 190 ℃, and leaving air temp is 85 ℃, and feed rate is 10 mL/min, gets product solid state powder shape dog grain flavouring agent.
(1) degrease freshly-slaughtered poultry brisket is twisted into meat gruel;
(2) in the meat gruel that step (1) obtains, add water by weight 1:4, fully stir;
(3) the meat slurry that step (2) is obtained is warming up to 50 ℃, regulates pH to 6.0～7.0, adds 1% flavor protease;
(4) meat slurry mechanical agitation 4 h under 50 ℃ of conditions that step (3) obtained survey degree of hydrolysis to 28%～32%; Then be warming up to rapidly 85 ℃～90 ℃, continue to stir 20 min;
(5) enzymolysis product that step (4) is obtained adds 1.0% chicken fat, 4.0% D-wood sugar, 1.0% Cys hydrochloride, 0.5% Cobastab
1, 1.0% food grade quality concentration 85% phosphoric acid after stir;
(6) product that step (5) is obtained is at 115 ℃ of lower continuous heating 50 min;
(7) product that step (6) is obtained stirs after adding 2% tara gum, 10% converted starch;
(8) spray-drying behind the product homogeneous that step (7) is obtained; Condition: EAT is 190 ℃, and leaving air temp is 85 ℃, and feed rate is 10 mL/min, gets product solid state powder shape dog grain flavouring agent.
The healthy adult beasle dog that approaches with 5 builds that satisfy the zoopery standard-required, body weight is as experimental dog; The place of feeding: medical college of Nantong University zoopery central standard kennel; The receptacle temperature is (23 ± 2) ℃, relative humidity (55 ± 5) %, light application time: 7:00～19:00, one in a cage.Carry out the palatability of experimental dog grain and test to determine optimum addition and the practical application effect of flavouring agent of the present invention.
1, experimental technique
(1) searches for food than test
For experimental dog at one time the section (9:00-15:00) two bowls are provided, put in each bowl with aliquot but different dog grain, experimental dog searching for food than (the ratio of feed intake and food total amount to two kinds of dog grains in the minute book time period, the % of unit), every other day bowl is contained dog grain and exchange, continue test.
(2) First preference test
For experimental dog provides two bowls at one time, put in each bowl with aliquot but different dog grain, observe and record when experimental dog is eaten First and select any dog grain, every other day bowl is contained dog grain and exchange, continue test.
2, experimental result
(1) optimum addition of dog grain flavouring agent of the present invention determines
For experimental dog provides a bowl, (the flavouring agent addition is respectively 0.5% of self-control basal diet quality wherein to fill a certain amount of dog grain flavouring agent of the present invention of interpolation, 1.0%, 2.0%, the self-control basal diet of equivalent 3.0%), the determination experiment dog is to the ratio of searching for food of the dog grain of interpolation different proportion flavouring agent.
Searching for food than the result of the experimental dog of the different dog grain of table 1 flavouring agent addition
Annotate: the alphabetical different person's significant difference (P＜0.05) of going together; Numerical value represents with mean+SD.
As can be seen from Table 1, when adding 0.5%, 1.0% and 3.0% dog grain flavouring agent, the searching for food than being 70% ~ 80% of experimental dog.When the addition of dog grain flavouring agent was 0.2%, searching for food than significantly improving of experimental dog can reach about 90%.Therefore, add the prepared dog grain flavouring agent of the inventive method improve experimental dog search for food than the time, the suitableeest addition is 2.0%.
(2) effect of dog grain flavouring agent of the present invention in the self-control basal diet
For experimental dog provides two bowls, a bowl fills the self-control basal diet, and the self-control basal diet of the interpolation 2.0% dog grain flavouring agent of equivalent is housed in the another bowl, the determination experiment dog search for food than with First preference, METHOD FOR CONTINUOUS DETERMINATION 5 days.
The First preference experimental result of table 2 dog grain flavouring agent in the self-control basal diet
As can be seen from Table 2, the experimental dog First of searching for food all selects to add the self-control basal diet of 2% dog grain flavouring agent, and the dog grain flavouring agent that the present invention's preparation is described has clear improvement to the local flavor of self-control basal diet.
As shown in Figure 1, fed continuously 5 days, experimental dog is searched for food to the self-control basal diet that adds 2% flavouring agent and is continued to be higher than the self-control basal diet (77.06% ~ 89.05%) that does not add flavouring agent than (22.94% ~ 10.95%), shows to add the palatability that dog grain flavouring agent of the present invention can improve the self-control basal diet.
(3) dog grain flavouring agent becomes effect in the dog grain at commercially available boccaccio
For experimental dog provides two bowls, a bowl fills boccaccio and becomes dog grain, and the commercially available boccaccio that the interpolation 2.0% dog grain flavouring agent of equivalent is housed in the another bowl becomes dog grain, carry out the determination experiment dog search for food than with First preference, METHOD FOR CONTINUOUS DETERMINATION 5 days.
Table 3 dog grain flavouring agent becomes First preference experimental result in the dog grain at commercially available boccaccio
As can be seen from Table 3, experimental dog becomes the First of dog grain selectively will be higher than boccaccio to the boccaccio that adds 2% dog grain flavouring agent and becomes dog grain, become the local flavor of dog grain to have clear improvement to commercially available boccaccio after the dog grain flavouring agent that adds the present invention's preparation is described, had a preference for by experimental dog.
As shown in Figure 2, the boccaccio that adds 2.0% dog grain flavouring agent becomes searching for food of dog grain also to be higher than the boccaccio that does not add flavouring agent than (65.78% ~ 66.14%) and becomes dog grain (34.22 ~ 33.86%).After explanation was added the dog grain flavouring agent of the present invention preparation commercially available boccaccio to and become dog grain, its palatability was better.
Claims (4)
1. solid state powder shape dog grain preparation of flavours method is characterized in that:
(1) degrease freshly-slaughtered poultry brisket is twisted into meat gruel;
(2) in the meat gruel that step (1) obtains, add water by weight 1:2～1:4, fully stir, get the meat slurry;
(3) the meat slurry that step (2) is obtained is warming up to 40 ℃～60 ℃, regulates pH to 5.0～7.0, adds the protease of incarnation slurry quality 1.0% ~ 3.0 %;
Described protease is papain or flavor protease;
(4) enzymolysis: the meat that step (3) obtains is starched at 40 ℃～60 ℃ lower mechanical agitation enzymolysis time 4～6 h, followed the tracks of and survey degree of hydrolysis to 28%～32%, then be warming up to rapidly 85 ℃～90 ℃, continue to stir 20 min, get enzymolysis liquid;
(5) in the enzymolysis liquid that step (4) obtains, add the phosphoric acid of enzymolysis liquid quality meter 0.3%～5% chicken fat, 2%～5% reduced sugar, 0.5%～5% Cys hydrochloride, 0～2% vitamin B1,0.5%～1% food grade quality concentration 85%, stir;
(6) product that step (5) is obtained is kept heating 40～50 min under 110 ℃～120 ℃;
(7) product that step (6) is obtained adds the tara gum of products therefrom quality meter 2%～5%, 10%～25% converted starch, stirs;
(8) spray-drying behind the product homogeneous that step (7) is obtained; Condition: EAT is 180 ℃～190 ℃, and leaving air temp is 80 ℃～90 ℃, and feed rate is 10 mL/min～15 mL/min, gets product solid state powder shape dog grain flavouring agent.
2. solid state powder shape dog grain preparation of flavours method according to claim 1 is characterized in that: the degree of hydrolysis of step (4) tracking and measuring enzymolysis liquid, and to follow the tracks of and survey degree of hydrolysis to 28%～32%, the strict controlled enzymatic hydrolysis time is 4 ~ 6 h.
3. solid state powder shape dog grain preparation of flavours method according to claim 1, it is characterized in that: the described reduced sugar of step (5) is the D-wood sugar.
4. the application of the solid state powder shape dog grain flavouring agent of the described method preparation of claim 1, it is characterized in that: make an addition in the dog grain after it is redissolved by mass concentration 30%～40%, can hide dog grain raw material itself intrinsic with and the bad smell that produced in process of production, the suitableeest addition of solid state powder shape dog grain flavouring agent is to account for 0.2% of dog grain quality.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103583829A (en) * | 2013-11-20 | 2014-02-19 | 上海美农生物科技股份有限公司 | Preparation method for flavoring agent of feed or pet food |
| CN105124319A (en) * | 2015-09-30 | 2015-12-09 | 江苏省协同医药生物工程有限责任公司 | Method for preparing dog food meat-flavor attractant |
| CN105192338A (en) * | 2014-06-30 | 2015-12-30 | 上海翼邦生物技术有限公司 | Preparation method of liquid-state chicken liver flavor dog food flavor agent |
| CN105212171A (en) * | 2014-07-01 | 2016-01-06 | 上海翼邦生物技术有限公司 | The preparation method of a kind of beef flavour solid-state powdery dog grain flavouring agent |
| CN105211655A (en) * | 2014-07-01 | 2016-01-06 | 上海翼邦生物技术有限公司 | A kind of preparation method of liquid fishiness cat grain flavouring agent |
| CN105265761A (en) * | 2014-06-30 | 2016-01-27 | 上海翼邦生物技术有限公司 | Liquid state chicken liver flavored cat food flavoring agent preparing method |
| CN106562073A (en) * | 2016-11-15 | 2017-04-19 | 上海翼邦生物技术有限公司 | Solid powdered feed additive and preparation method thereof |
| CN107047941A (en) * | 2016-12-14 | 2017-08-18 | 江苏雅博动物保健品有限责任公司 | A kind of preparation method of powdered dog flavouring agent |
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| CN103583829A (en) * | 2013-11-20 | 2014-02-19 | 上海美农生物科技股份有限公司 | Preparation method for flavoring agent of feed or pet food |
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| CN105192338A (en) * | 2014-06-30 | 2015-12-30 | 上海翼邦生物技术有限公司 | Preparation method of liquid-state chicken liver flavor dog food flavor agent |
| CN105265761A (en) * | 2014-06-30 | 2016-01-27 | 上海翼邦生物技术有限公司 | Liquid state chicken liver flavored cat food flavoring agent preparing method |
| CN105212171A (en) * | 2014-07-01 | 2016-01-06 | 上海翼邦生物技术有限公司 | The preparation method of a kind of beef flavour solid-state powdery dog grain flavouring agent |
| CN105211655A (en) * | 2014-07-01 | 2016-01-06 | 上海翼邦生物技术有限公司 | A kind of preparation method of liquid fishiness cat grain flavouring agent |
| CN105124319A (en) * | 2015-09-30 | 2015-12-09 | 江苏省协同医药生物工程有限责任公司 | Method for preparing dog food meat-flavor attractant |
| CN106562073A (en) * | 2016-11-15 | 2017-04-19 | 上海翼邦生物技术有限公司 | Solid powdered feed additive and preparation method thereof |
| CN107047941A (en) * | 2016-12-14 | 2017-08-18 | 江苏雅博动物保健品有限责任公司 | A kind of preparation method of powdered dog flavouring agent |
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Application publication date: 20130403 |