CN103004802A - Biological fungicide composition and application thereof - Google Patents
Biological fungicide composition and application thereof Download PDFInfo
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- CN103004802A CN103004802A CN2013100140110A CN201310014011A CN103004802A CN 103004802 A CN103004802 A CN 103004802A CN 2013100140110 A CN2013100140110 A CN 2013100140110A CN 201310014011 A CN201310014011 A CN 201310014011A CN 103004802 A CN103004802 A CN 103004802A
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Abstract
The invention relates to the technical field of pesticide, and particularly relates to a biological fungicide composition. The biological fungicide composition contains zhongshengrnycin and kasugamycin, wherein the weight ratio of the zhongshengrnycin to the kasugamycin is 1: (0.2-7). The fungicide composition disclosed by the invention is significant in synergized action, high in field efficacy, and wide in bacteriocidal spectrum, and has good control effect on rice blast, fusarium wilt of cucumber, cucumber downy mildew, bacterial angular leaf spot of cucumber, tomato leaf mould, tomato bacterial macula diseases, pumpkin soft rot, watermelon bacterial angular spot, bacterial blotch of watermelon, stuffed watermelon water melon, pepper anthracnose, hot (sweet) pepper bacterial leaf spot, capsicum powdery mildew, pepper grey mould, citrus canker, litchi downy blight, peach tree gummosis, scab of peach and peach shot hole. The control cost is reduced.
Description
Technical field
The present invention relates to technical field of pesticide, be specifically related to a kind of biological bactericide composition, also relate to the application of biological bactericide composition.
Background technology
In agricultural production practice, bacterial diseases of plants and fungal disease are more and more heavier, and usually mixed generation.In order to alleviate the harm of germ, often widely apply bactericide.Some bactericide is owing to prolonged application, and germ has produced pesticide resistance so that preventive effect descends, and has to strengthen consumption.Some bactericide specificity is forced to such an extent that the control spectrum is narrower, and the peasant must use together in the various sterilization agent.The abuse of chemical bactericide has caused serious environmental pollution and food-safety problem.The exploitation of novel biological bactericide is the important channel of cracking above-mentioned dilemma.
Zhongshengmycin (zhongshengmycin) belongs to N-glucosides class, is a kind of agricultural bactericidal antibiotic of wide spectrum, by suppressing the synthetic of bacterium protein and make filamentous fungi mycelia deformity, thereby suppresses spore germination and killing spores and causes germ dead.Can resisting gram-positive, negative bacteria, mycobacterium, saccharomycete and filamentous fungi.Generation bacterium of Zhongshengmycin and preparation method thereof is provided by patent ZL200310103250.X, this disclosure of the Invention lilac grey streptomycete Hainan mutation CGMCC No.1026, and by obtaining Zhongshengmycin after the CGMCC No.1026 fermentation, provide simultaneously the method that is prepared Zhongshengmycin by this bacterial classification.Patent ZL200310103513.7 then provides the wetting powder of Zhongshengmycin to form and preparation method thereof.Patent ZL200510044505.9 provides a kind of agricultural bactericidal composition for plant disease, and said composition is by Zhongshengmycin and copper acetate is composite forms.Zhongshengmycin is a class new antibiotic, and various bacterial disease and the fungal disease of crops all had preferably effect.But on the whole, the complex preparation kind of Zhongshengmycin is less at present, is badly in need of its composite product of exploitation, enlarges its fungicidal spectrum.
Kasugarnycin (kasugamycin) has another name called kasugarnycin, kasumin, is a kind of interior absorption biological bactericide that has protection and therapeutic action concurrently.Kasugarnycin comes the synthetic of Profilin matter by the 30S-mRNA of interference aminoacyl-tRNA and ribosomal subunit's compound and the combination of 70S-mRNA, thereby suppresses the mycelia elongation and cause cell granulations, but spore germination is not had impact.Kasugarnycin has special efficacy to rice blast, and cladosporium leaf and fruit mould of tomato and apple scab are also had certain effect.The kasugarnycin permeability is strong, and resistance of rainwater washing against is to the person poultry safety.But because Pyricularia oryzae is very fast that kasugarnycin has been produced pesticide resistance, and kasugarnycin produces poisoning easily to the part crop, causes the use value of kasugarnycin to reduce.Kasugarnycin mixes the shortcoming that use can effectively overcome this medicament from the bactericide of the different modes of action.
Summary of the invention
In order to solve the problems such as above-mentioned Zhongshengmycin complex preparation kind is few, kasugarnycin easily produces poisoning and germ develops immunity to drugs, the invention provides a kind of take Zhongshengmycin and kasugarnycin as active ingredient, the double biological bactericide composition of controlling bacterial diseases of plants and fungal disease.
The present invention also provides the application of described biological bactericide composition.
The present invention is achieved by the following measures:
A kind of biological bactericide composition contains Zhongshengmycin and kasugarnycin, and the weight ratio of Zhongshengmycin and kasugarnycin is 1:0.2～7.
Described biological bactericide composition, the weight ratio of preferred Zhongshengmycin and kasugarnycin is 1:0.3～3.
Described biological bactericide composition, the weight ratio of preferred Zhongshengmycin and kasugarnycin is 3:2.
Described biological bactericide composition also contains auxiliary agent and carrier, makes wetting powder.Auxiliary agent mainly plays wetting, peptizaiton, and a surface-active action is provided, and makes its dispersion more even; Carrier mainly plays absorption, filling effect.Employed auxiliary agent and carrier in the microbicide compositions of the present invention, be wetting powder produce in auxiliary agent and carrier commonly used, neopelex auxiliary agent for example, precipitated calcium carbonate carrier.
Described biological bactericide composition, the weight percentage of preferred feedstock is as follows: Zhongshengmycin 0.1～15%, kasugarnycin 0.1～15%, auxiliary agent 1～10%, carrier 60～98.8%.
Described biological bactericide composition, the weight percentage of preferred feedstock is as follows: Zhongshengmycin 0.2～6%, kasugarnycin 0.2～8%, auxiliary agent 1～8%, carrier 78～98.6%.
Described biological bactericide composition is raw material to be added in the mixer mix, and through precomminution, pulverize, sieve, obtains.
Described biological bactericide composition is used for control rice blast, cucumber fusarium axysporum, cucumber downy mildew, cucumber bacterial angular leaf spot, cladosporium leaf and fruit mould of tomato, tomato bacterial speck, custard squash soft rot, watermelon bacterial angular leaf spot, bacterial fruit blotch of watermelon, watermelon water flesh melon, pepper anthracnose, peppery (sweet) green pepper bacterial leaf spot, pepper powdery mildew, Botrytis cinerea, mandarin tree canker, peronophythora litchi, peach gummosis, peach scab and peach shot hole, can obtain good effect.
Described application, for converting the water of 300～1000 times of weight, mixing is made into medicine liquid spray.
Show through test of many times, after Zhongshengmycin and kasugarnycin are composite by a certain percentage, can produce obvious synergistic effect.After Zhongshengmycin and kasugarnycin are composite according to weight ratio 3:2, use general method and carry out indoor bioassay, is 146.83 to the co-toxicity coefficient of cucumber bacterial angular leaf spot bacterium, and synergy is remarkable.Field trial shows that the effect of 700 times of liquid controls of microbicide compositions provided by the invention cucumber bacterial angular leaf spot reaches 93%, far above 81% preventive effect of contrast medicament Zhongshengmycin and 72% preventive effect of kasugarnycin.The presentation of results of indoor bioassay and field trial, the synergy of said components obviously improve bactericidal activity of the present invention.
After Zhongshengmycin and kasugarnycin were composite, fungicidal spectrum obviously enlarged.This composition is to rice blast, cucumber fusarium axysporum, cucumber downy mildew, cucumber bacterial angular leaf spot, cladosporium leaf and fruit mould of tomato, tomato bacterial speck, custard squash soft rot, watermelon bacterial angular leaf spot, bacterial fruit blotch of watermelon, watermelon water flesh melon, and pepper anthracnose, peppery (sweet) green pepper bacterial leaf spot, pepper powdery mildew, Botrytis cinerea, mandarin tree canker, peronophythora litchi, peach gummosis, peach scab and peach shot hole have good control efficiency.
Beneficial effect of the present invention:
1, biological bactericide composition of the present invention synergistic effect is remarkable, and field efficacy is high.This composition is converted the water of 700 times of weight, during the even spraying of blade face, the effect of cucumber bacterial angular leaf spot is reached 93%.
2, fungicidal spectrum is wide.Control rice blast, cucumber fusarium axysporum, cucumber downy mildew, cucumber bacterial angular leaf spot, cladosporium leaf and fruit mould of tomato, tomato bacterial speck, custard squash soft rot, watermelon bacterial angular leaf spot, bacterial fruit blotch of watermelon, watermelon water flesh melon, pepper anthracnose, peppery (sweet) green pepper bacterial leaf spot, pepper powdery mildew, Botrytis cinerea, mandarin tree canker, peronophythora litchi, peach gummosis, peach scab and peach shot hole can be obtained good effect.For example 700 times of liquid of this built agent reach 89% to the field efficacy of tomato bacterial speck, and the field efficacy of custard squash soft rot is reached 86%.700 times of liquid foliar sprays reach 87% to the field control effect of fungal disease cladosporium leaf and fruit mould of tomato.This explanation, Zhongshengmycin and kasugarnycin composite, not only synergy is obvious, and has enlarged fungicidal spectrum, and prevention and treatment range obviously enlarges.
3, reduced expenses for prevention and control.Because field general multiple diseases mixed occurrence, particularly fungal disease and the frequent mixed occurrence of bacteriosis, use this composite bactericide Zhiduo County's kind disease of holding concurrently, this has not only reduced drug cost, has also saved peasant's labour;
4, technical threshold is low, and production technology is simple.
Embodiment
For a better understanding of the present invention, further specify below in conjunction with specific embodiment.500 times of liquid among the following embodiment, 700 times of liquid and 900 times of liquid refer to that the wetting powder that will obtain adds the liquid that mixing obtains in the water of 500 times, 700 times and 900 times weight.
Embodiment 1
Each component is gone out wetting powder according to following percentage by weight by above-mentioned explained hereafter: Zhongshengmycin 3%, kasugarnycin 2%, auxiliary agent 5%, carrier 90%.Medicament contrast is established 2, is respectively and contains Zhongshengmycin 3%, auxiliary agent 5%, the medicament contrast 1 of carrier 92% and contain kasugarnycin 2%, auxiliary agent 5%, the medicament contrast 2 of carrier 93%.Blank is the clear water spraying.Carry out cucumber bacterial angular leaf spot control test at Shouguang City, Shandong discipline platform town winter warm type big shack year June in October, 2008～2009.500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid and medicament are set to be contrasted 1 wetting powder 700 times of liquid, medicaments and contrasts 700 times of liquid of 2 wetting powders and blank treatment region.Foliar spray, spray in per 7 days 1 time connects and sprays 4 times.Residential quarter area 30m
2, repeating 4 times, district's group is arranged at random.Wherein the field control effect processed cucumber bacterial angular leaf spot of 500 times of liquid of this wetting powder, 700 times of liquid and 900 times of liquid is respectively 95%, 93% and 82%.And medicament to contrast the spraying of 700 times of liquid of 1 wetting powder be 81% to the field control effect of cucumber bacterial angular leaf spot, it only is 72% to the field control effect of cucumber bacterial angular leaf spot that medicament contrasts the spraying of 700 times of liquid of 2 wetting powders, and the two composite rear preventive effect obviously improves.
Embodiment 2
Each component is gone out wetting powder according to following percentage by weight by above-mentioned explained hereafter: Zhongshengmycin 2%, kasugarnycin 3%, auxiliary agent 5%, carrier 90%.Medicament contrast is established 2, is respectively and contains Zhongshengmycin 2%, auxiliary agent 5%, the medicament contrast 1 of carrier 93% and contain kasugarnycin 3%, auxiliary agent 5%, the medicament contrast 2 of carrier 92%.Blank is the clear water spraying.In Linzi, Shandong imperial city town winter warm type big shack, carry out tomato bacterial speck control test year June in October, 2009～2010.500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid and medicament are set to be contrasted 1 wetting powder 700 times of liquid, medicaments and contrasts 700 times of liquid of 2 wetting powders and blank treatment region.Foliar spray, spray in per 7 days 1 time connects and sprays 3 times.Residential quarter area 30 m
2, repeating 4 times, district's group is arranged at random.Wherein the field control effect processed tomato bacterial speck of 500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid is respectively 91%, 89% and 82%.And medicament to contrast the spraying of 700 times of liquid of 1 wetting powder be 80% to the field control effect of tomato bacterial speck, it only is 65% to the field control effect of tomato bacterial speck that medicament contrasts the spraying of 700 times of liquid of 2 wetting powders, and the two composite rear preventive effect obviously improves.
Embodiment 3
Each component is gone out wetting powder according to following percentage by weight by above-mentioned explained hereafter: Zhongshengmycin 2%, kasugarnycin 4%, auxiliary agent 5%, carrier 89%.Medicament contrast is established 2, is respectively and contains Zhongshengmycin 2%, auxiliary agent 5%, the medicament contrast 1 of carrier 93% and contain kasugarnycin 4%, auxiliary agent 5%, the medicament contrast 2 of carrier 91%.Blank is the clear water spraying.In Qu Di town, Jiyang Area, Shandong county winter warm type big shack, carry out cladosporium leaf and fruit mould of tomato control test year July in September, 2008～2009.500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid and medicament are set to be contrasted 1 wetting powder 700 times of liquid, medicaments and contrasts 700 times of liquid of 2 wetting powders and blank treatment region.Foliar spray, spray in per 7 days 1 time connects and sprays 4 times.Residential quarter area 25 m
2, repeating 4 times, district's group is arranged at random.Wherein the field control effect processed cladosporium leaf and fruit mould of tomato of 500 times of liquid of this wetting powder, 700 times of liquid and 900 times of liquid is respectively 90%, 87% and 82%.And medicament to contrast the spraying of 700 times of liquid of 1 wetting powder only be 54% to the field control effect of cladosporium leaf and fruit mould of tomato, it is 78% to the field control effect of cladosporium leaf and fruit mould of tomato that medicament contrasts 700 times of liquid sprayings of 2 wetting powders, the two composite rear preventive effect obviously improves.
Embodiment 4
Each component is gone out wetting powder according to following percentage by weight by above-mentioned explained hereafter: Zhongshengmycin 3%, kasugarnycin 1%, auxiliary agent 4%, carrier 92%.Medicament contrast is established 2, is respectively and contains Zhongshengmycin 3%, auxiliary agent 4%, the medicament contrast 1 of carrier 93% and contain kasugarnycin 1%, auxiliary agent 5%, the medicament contrast 2 of carrier 94%.Blank is the clear water spraying.Carry out the custard squash Experiment of Disease-Control for Soft-Rotting on high-ranking official stockaded village, Zhangqiu City, Shandong Province year May in October, 2009～2010.500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid and medicament are set to be contrasted 1 wetting powder 700 times of liquid, medicaments and contrasts 700 times of liquid of 2 wetting powders and blank treatment region.Foliar spray, spray in per 7 days 1 time connects and sprays 3 times.Residential quarter area 40 m
2, repeating 3 times, district's group is arranged at random.Wherein the field control effect processed the custard squash soft rot of 500 times of liquid of this wetting powder, 700 times of liquid and 900 times of liquid is respectively 90%, 86% and 81%.And medicament to contrast the spraying of 700 times of liquid of 1 wetting powder be 80% to the field control effect of custard squash soft rot, it only is 52% to the field control effect of custard squash soft rot that medicament contrasts 700 times of liquid sprayings of 2 wetting powders, the two composite rear preventive effect obviously improves.
Embodiment 5
Each component is gone out wetting powder according to following percentage by weight by above-mentioned explained hereafter: Zhongshengmycin 1%, kasugarnycin 5%, auxiliary agent 5%, carrier 89%.Medicament contrast is established 2, is respectively and contains Zhongshengmycin 1%, auxiliary agent 5%, the medicament contrast 1 of carrier 94% and contain kasugarnycin 5%, auxiliary agent 5%, the medicament contrast 2 of carrier 90%.Blank is the clear water spraying.Carried out the control test of watermelon water flesh melon year July in Yao Gou town, Changyue County, Shandong Province in March, 2009～2009.Glad No. two of test kind capital.500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid and medicament are set to be contrasted 1 wetting powder 700 times of liquid, medicaments and contrasts 700 times of liquid of 2 wetting powders and blank treatment region.Hand-rail type high-pressure sprayer foliar spray, spray in per 10 days 1 time connects and sprays 3 times.Residential quarter area 50m
2, repeating 4 times, district's group is arranged at random.Wherein the field control effect processed watermelon water flesh melon of 500 times of liquid of this wetting powder, 700 times of liquid and 900 times of liquid is respectively 90%, 87% and 78%.And medicament to contrast the spraying of 700 times of liquid of 1 wetting powder be 76% to the field control effect of watermelon water flesh melon, it only is 61% to the field control effect of watermelon water flesh melon that medicament contrasts 700 times of liquid sprayings of 2 wetting powders, the two composite rear preventive effect obviously improves.
Embodiment 6
Each component is gone out wetting powder according to following percentage by weight by above-mentioned explained hereafter: Zhongshengmycin 5%, kasugarnycin 1%, auxiliary agent 9%, carrier 85%.Medicament contrast is established 2, is respectively and contains Zhongshengmycin 5%, auxiliary agent 9%, the medicament contrast 1 of carrier 86% and contain kasugarnycin 1%, auxiliary agent 9%, the medicament contrast 2 of carrier 90%.Blank is the clear water spraying.Carried out Botrytis cinerea control test year March in the Cangshan County, Shandong Province in October, 2010～2011.500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid and medicament are set to be contrasted 1 wetting powder 700 times of liquid, medicaments and contrasts 700 times of liquid of 2 wetting powders and blank treatment region.Foliar spray, spray in per 7 days 1 time connects and sprays 3 times.Residential quarter area 25 m
2, repeating 3 times, district's group is arranged at random.Wherein the field control effect processed Botrytis cinerea of 500 times of liquid of this wetting powder, 700 times of liquid and 900 times of liquid is respectively 86%, 83% and 75%.And medicament to contrast the spraying of 700 times of liquid of 1 wetting powder be 62% to the field control effect of Botrytis cinerea, it is 55% to the field control effect of Botrytis cinerea that medicament contrasts 700 times of liquid sprayings of 2 wetting powders, the two composite rear preventive effect obviously improves.
Embodiment 7
Each component is gone out wetting powder according to following percentage by weight by above-mentioned explained hereafter: Zhongshengmycin 1%, kasugarnycin 7%, auxiliary agent 8%, carrier 84%.Medicament contrast is established 2, is respectively and contains Zhongshengmycin 1%, auxiliary agent 8%, the medicament contrast 1 of carrier 91% and contain kasugarnycin 7%, auxiliary agent 8%, the medicament contrast 2 of carrier 85%.Blank is the clear water spraying.Carried out the control test of rice blast year October at Jining City in Shandong Province in July, 2011～2011.500 times of liquid of this wetting powder, 700 times of liquid, 900 times of liquid and medicament are set to be contrasted 1 wetting powder 700 times of liquid, medicaments and contrasts 700 times of liquid of 2 wetting powders and blank treatment region.Foliar spray, spray in per 7 days 1 time connects and sprays 3 times.Residential quarter area 25 m
2, repeating 3 times, district's group is arranged at random.Wherein the field control effect processed rice blast of 500 times of liquid of this wetting powder, 700 times of liquid and 900 times of liquid is respectively 88%, 84% and 79%.And medicament to contrast the spraying of 700 times of liquid of 1 wetting powder be 46% to the field control effect of rice blast, it is 75% to the field control effect of rice blast that medicament contrasts 700 times of liquid sprayings of 2 wetting powders, the two composite rear preventive effect obviously improves.
The wetting powder of microbicide compositions of the present invention in preparation above-described embodiment, also can make other formulations, such as liquid preparation.The same with wetting powder, make other formulation after, the two composite not only can synergy, and can the expanding prevention spectrum.
Claims (10)
1. a biological bactericide composition is characterized in that containing Zhongshengmycin and kasugarnycin, and the weight ratio of Zhongshengmycin and kasugarnycin is 1:0.2～7.
2. biological bactericide composition according to claim 1, the weight ratio that it is characterized in that Zhongshengmycin and kasugarnycin is 1:0.3～3.
3. biological bactericide composition according to claim 1, the weight ratio that it is characterized in that Zhongshengmycin and kasugarnycin is 3:2.
4. biological bactericide composition according to claim 1 is characterized in that also containing auxiliary agent and carrier.
5. biological bactericide composition according to claim 1 is characterized in that the weight percentage of raw material is as follows: Zhongshengmycin 0.1～15%, kasugarnycin 0.1～15%, auxiliary agent 1～10%, carrier 60～98.8%.
6. biological bactericide composition according to claim 1 is characterized in that the weight percentage of raw material is as follows: Zhongshengmycin 0.2～6%, kasugarnycin 0.2～8%, auxiliary agent 1～8%, carrier 78～98.6%.
7. each described biological bactericide composition according to claim 1～6 is characterized in that raw material added in the mixer and mixes, and through precomminution, pulverize, sieve, and get final product.
8. according to claim 4, each described biological bactericide composition in 5 or 6, it is characterized in that being wetting powder, described auxiliary agent is neopelex, carrier is precipitated calcium carbonate.
In the claim 1～8 each described biological bactericide composition at the control rice blast, cucumber fusarium axysporum, cucumber downy mildew, cucumber bacterial angular leaf spot, cladosporium leaf and fruit mould of tomato, tomato bacterial speck, the custard squash soft rot, the watermelon bacterial angular leaf spot, bacterial fruit blotch of watermelon, watermelon water flesh melon, pepper anthracnose, the capsicum bacterial leaf spot, the pimento bacterial leaf spot, pepper powdery mildew, Botrytis cinerea, the mandarin tree canker, peronophythora litchi, peach gummosis, application in peach scab and the peach shot hole.
10. application according to claim 9 is characterized in that converting the water of 300～1000 times of weight, and mixing is made into medicine liquid spray.
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Cited By (4)
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| CN103598192A (en) * | 2013-11-30 | 2014-02-26 | 海利尔药业集团股份有限公司 | Bactericidal composition containing tetramycin and zhongshengmycin |
| CN109122682A (en) * | 2017-06-16 | 2019-01-04 | 桂林集琦生化有限公司 | A kind of agricultural synergist and Zhongshengmycin, the mixture of kasugarnycin compound mixture and its preparation and application |
| CN112616583A (en) * | 2020-12-14 | 2021-04-09 | 安徽金敦福农业科技有限公司 | Method for preventing and treating bacterial fruit blotch of watermelons |
| CN115067346A (en) * | 2022-04-28 | 2022-09-20 | 湖南农业大学 | Seed coating agent for preventing and treating watermelon fruit blotch and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103598192A (en) * | 2013-11-30 | 2014-02-26 | 海利尔药业集团股份有限公司 | Bactericidal composition containing tetramycin and zhongshengmycin |
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| CN109122682A (en) * | 2017-06-16 | 2019-01-04 | 桂林集琦生化有限公司 | A kind of agricultural synergist and Zhongshengmycin, the mixture of kasugarnycin compound mixture and its preparation and application |
| CN109122682B (en) * | 2017-06-16 | 2020-11-03 | 桂林集琦生化有限公司 | Mixture prepared by mixing agricultural synergist with Zhongshengmycin and kasugamycin compound and preparation and application thereof |
| CN112616583A (en) * | 2020-12-14 | 2021-04-09 | 安徽金敦福农业科技有限公司 | Method for preventing and treating bacterial fruit blotch of watermelons |
| CN115067346A (en) * | 2022-04-28 | 2022-09-20 | 湖南农业大学 | Seed coating agent for preventing and treating watermelon fruit blotch and preparation method thereof |
| CN115067346B (en) * | 2022-04-28 | 2024-03-01 | 湖南农业大学 | Seed coating agent for preventing and treating watermelon fruit blotch and preparation method thereof |
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| CN103004802B (en) | 2014-12-24 |
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