CN102994565B - Method for enhancing anaerobic fermentation of algal waste liquid to generate methane - Google Patents
Method for enhancing anaerobic fermentation of algal waste liquid to generate methane Download PDFInfo
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- CN102994565B CN102994565B CN201210555014.0A CN201210555014A CN102994565B CN 102994565 B CN102994565 B CN 102994565B CN 201210555014 A CN201210555014 A CN 201210555014A CN 102994565 B CN102994565 B CN 102994565B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention relates to a method for enhancing anaerobic fermentation of algal waste liquid to generate methane. The method comprises the following steps of: firstly adopting a centrifugation method to extract extracellular polymeric substances of anaerobic sludge; mixing and adding the algal waste liquid and anaerobic sludge into an anaerobic bioreactor; adjusting the nutritive ratio of C/N/P in the anaerobic bioreactor, wherein the inflowing water quality after the nutritive ratio is adjusted is as follows: COD is 20000-25000mg/L, C/N/P is (100-200):5:1, and PH value is 6.0-9.0; and after preheating, preparing the extracted extracellular polymeric substances and NiCL2.6H2O into mixed liquid A and adding the mixed liquid A into the anaerobic bioreactor. The method has the advantages that necessary trace element Ni for growing the methanogen is chelated into a dissolved-state biological promoter for utilization of the methanogen, the chemical precipitation action is overcome, the utilization ratio of the trace element is increased, the gas generation ratio for anaerobic fermentation of the algal waste liquid is increased to the maximum extent, the adding amount of the promoter is less, the cost is low and the operation is simple.
Description
Technical field
The present invention relates to a kind of water technology, particularly a kind of method promoting algae waste liquid producing methane through anaerobic fermentation.
Background technology
The microorganism amount reproduction such as blue-green algae after seawater or poisons in freshwater eutrophication, thus red tide or wawter bloom phenomenon can be caused respectively, as big area wawter bloom appears in May, 2008 Taihu Lake, wawter bloom often processes in the mode of manually salvaging prolific algae after occurring, the filtrate organic content that the algae of collecting produces after press filtration is high, and needing to carry out process could discharge water body.Producing methane through anaerobic fermentation is a kind of economy, the feasible resource reutilization method of this kind of waste liquid of process.
In algae waste liquid anaerobic fermentation process, the growth of methanogen requires in suitable scope except C/N/P nutrition-allocated proportion, also needs the micro-metals of some necessity.There are some researches show, the interpolation of micro-metals Ni can promote the raising of biogas yield.But due to the existence of anaerobic reactor medium sulphide content, vitriol, phosphoric acid salt or carbonate, easily cause the chemical precipitation of Ni element.Therefore, find a kind of desirable sequestrant, Ni element is sequestered in inner complex inside, make it be present in anaerobic reactor with the form of solubilised state, utilize for methanogen, just become the key issue improving algae waste liquid anaerobic fermentation gas production rate.
Summary of the invention
The technical problem to be solved in the present invention is for the deficiencies in the prior art, proposes a kind of method overcoming chemical precipitation, improve the promotion algae waste liquid producing methane through anaerobic fermentation of the utilization ratio of trace element.
The technical problem to be solved in the present invention is achieved through the following technical solutions, and a kind of method promoting algae waste liquid producing methane through anaerobic fermentation, is characterized in:
First adopt centrifuging to extract the extracellular polymeric EPS of anaerobic sludge, take the anaerobic sludge mixed solution of mass concentration 8 ~ 9g/L, centrifugal 10 ~ 15min under the condition of 3000 ~ 3500r/min, abandoning supernatant is also supplied with distilled water, twice like this; Centrifugal 10 ~ 15min again under the condition of 3000 ~ 3500r/min, direct high speed centrifugation 10 ~ 15min under the condition of 10000 ~ 12000r/min after abandoning supernatant, gets the supernatant liquor after high speed centrifugation and can obtain extracellular polymeric EPS after 0.22 μm of membrane filtration; In extracellular polymeric EPS, organic content is 23% ~ 61%, protein content is 2.1% ~ 28.3%, saccharan content is 1.0% ~ 23.0%, DNA content is 0% ~ 5.2%;
Algae waste liquid and anaerobic sludge are mixed to join in anaerobic biological reactor UASB, the concentration making anaerobic sludge account for total amount of liquid is 8 ~ 9g/L, again the C/N/P nutrition ratio in anaerobic biological reactor UASB is adjusted, concrete grammar is: use methyl alcohol to change the ratio of organic carbon, urea is used to change the ratio of nitrogen, phosphoric acid salt is used to change the ratio of phosphorus, adjustment nutrition than rear influent quality is: COD is 20000 ~ 25000mg/L, C/N/P is 100 ~ 200:5:1, pH value is 6.0 ~ 9.0, the method of calrod to water inlet preheating is adopted to make temperature control in anaerobic biological reactor UASB be 50 ~ 55 DEG C, by extracellular polymeric EPS and the NiCL extracted
2.6H
2the ratio of O 5:1 ~ 10:1 is in mass ratio mixed and made into mixed liquor A, then is added in anaerobic biological reactor UASB by the proportional concentration of mixed liquor A by 30 ~ 50 μ L/L.
The technical problem to be solved in the present invention can also be come to realize further by the following technical programs, is muddy water admixture in anaerobic biological reactor UASB, and the upwelling that hybrid power is intake from bottom drives.
Beneficial effect of the present invention: key point of the present invention is to extract extracellular polymeric EPS as sequestrant from the anaerobic sludge of sewage treatment process structures anaerobic biological reactor UASB inside, methanogen is grown necessary micro-Ni and be chelated to solubilised state Biostimulation agent for methanogen utilization, overcome chemical precipitation, improve the utilization ratio of trace element.Sequestrant comes from the inner microorganism of anaerobic reactor and produces, with reactor system, there is high-adaptability, at utmost improve the factor of created gase of algae waste liquid anaerobically fermenting, factor of created gase improves 53% ~ 72%, and promotor addition is small, and cost is low, simple to operate, successful, can be used for engineering practice.
Embodiment
A kind of method promoting algae waste liquid producing methane through anaerobic fermentation, algae waste liquid producing methane through anaerobic fermentation process nature is methanogen in anaerobic biological reactor utilizes process to organic substrates, and methanogen is present in inside reactor with the form of anaerobic sludge.So, should extract from anaerobic sludge in the chosen process of sequestrant, at utmost could adapt to the system environment in anaerobic reactor.
First adopt centrifuging to extract the extracellular polymeric EPS of anaerobic sludge, take the anaerobic sludge mixed solution of mass concentration 8 ~ 9g/L, centrifugal 10 ~ 15min under the condition of 3000 ~ 3500r/min, abandoning supernatant is also supplied with distilled water, twice like this; Centrifugal 10 ~ 15min again under the condition of 3000 ~ 3500r/min, direct high speed centrifugation 10 ~ 15min under the condition of 10000 ~ 12000r/min after abandoning supernatant, gets the supernatant liquor after high speed centrifugation and can obtain extracellular polymeric EPS after 0.22 μm of membrane filtration; In extracellular polymeric EPS, organic content is 23% ~ 61%, protein content is 2.1% ~ 28.3%, saccharan content is 1.0% ~ 23.0%, DNA content is 0% ~ 5.2%;
Algae waste liquid and anaerobic sludge are mixed to join in anaerobic biological reactor UASB, the concentration making anaerobic sludge account for total amount of liquid is 8 ~ 9g/L, again the C/N/P nutrition ratio in anaerobic biological reactor UASB is adjusted, concrete grammar is: use methyl alcohol to change the ratio of organic carbon, urea is used to change the ratio of nitrogen, phosphoric acid salt is used to change the ratio of phosphorus, adjustment nutrition than rear influent quality is: COD (chemical oxygen demand (COD)) is 20000 ~ 25000mg/L, C/N/P is 100 ~ 200:5:1, pH value is 6.0 ~ 9.0, the method of calrod to water inlet preheating is adopted to make temperature control in anaerobic biological reactor UASB be 50 ~ 55 DEG C, by extracellular polymeric EPS and the NiCL extracted
2.6H
2the ratio of O 5:1 ~ 10:1 is in mass ratio mixed and made into mixed liquor A, again mixed liquor A is added in anaerobic biological reactor UASB by the proportional concentration of 30 ~ 50 μ L/L, be muddy water admixture in anaerobic biological reactor UASB, the upwelling that hybrid power is intake from bottom drives.
Mixed liquor A is added in anaerobic biological reactor UASB by the proportional concentration of 30 ~ 50 μ L/L, and the meaning is exactly: in mixed liquor A add-on and anaerobic biological reactor UASB, the ratio of total amount of liquid is 30 ~ 50 μ L/L.Namely the amount adding mixed liquor A in the liquid in every L anaerobic biological reactor UASB is 30 ~ 50 μ L.
Described anaerobic sludge takes from city domestic sewage treatment plant, city domestic sewage after anaerobic biochemical treatment, the throw out that the Institute of Micro-biology that grows in reactor is formed.
Just the present invention illustrates below, but is not limitation of the present invention.
Case of comparative examples:
The biogas yield that algae waste liquid uniform temp under the condition of not adding Biostimulation agent carries out anaerobically fermenting is 0.32m
3/ kgCOD.
Embodiment 1
Algae waste liquid COD is 21100mg/L, C/N/P be 180:5:1, PH is 6.7, and initial anaerobic sludge concentration is 8.2g/L, and temperature is 50 ~ 55 DEG C.Extracellular polymeric EPS organic content is 31%, and wherein protein content is 8.1%, and saccharan is 6.5%, DNA is 0.7%.EPS and NiCL in mixed liquor A
2.6H
2the mass ratio of O is 10:1, and the concentration that mixed liquor A adds in UASB is 30 μ L/L.Measure under the same terms and measuring method, biogas yield is 0.49 m
3/ kgCOD, improves 53% than reference examples.
Embodiment 2
Algae waste liquid COD is 24300mg/L, C/N/P be 200:5:1, PH is 8.2, and initial anaerobic sludge concentration is 8.5g/L, and temperature is 50 ~ 55 DEG C.Extracellular polymeric EPS organic content is 61%, and wherein protein content is 10.7%, and saccharan is 8.2%, DNA is 1.9%.EPS and NiCL in mixed liquor A
2.6H
2the mass ratio of O is 5:1, and the concentration that mixed liquor A adds in UASB is 50 μ L/L.Measure under the same terms and measuring method, biogas yield is 0.55 m
3/ kgCOD, improves 72% than reference examples.
Claims (2)
1. one kind promotes the method for algae waste liquid producing methane through anaerobic fermentation, it is characterized in that: first adopt centrifuging to extract the extracellular polymeric of anaerobic sludge, take the anaerobic sludge mixed solution of mass concentration 8 ~ 9g/L, centrifugal 10 ~ 15min under the condition of 3000 ~ 3500r/min, abandoning supernatant is also supplied with distilled water, twice like this; Centrifugal 10 ~ 15min again under the condition of 3000 ~ 3500r/min, direct high speed centrifugation 10 ~ 15min under the condition of 10000 ~ 12000r/min after abandoning supernatant, gets the supernatant liquor after high speed centrifugation and can obtain extracellular polymeric after 0.22 μm of membrane filtration; In extracellular polymeric, organic content is 23% ~ 61%, protein content is 2.1% ~ 28.3%, saccharan content is 1.0% ~ 23.0%, DNA content is 0% ~ 5.2%;
Algae waste liquid and anaerobic sludge are mixed to join in anaerobic biological reactor, the concentration making anaerobic sludge account for total amount of liquid is 8 ~ 9g/L, again the C/N/P nutrition ratio in anaerobic biological reactor is adjusted, concrete grammar is: use methyl alcohol to change the ratio of organic carbon, urea is used to change the ratio of nitrogen, phosphoric acid salt is used to change the ratio of phosphorus, adjustment nutrition than rear influent quality is: COD is 20000 ~ 25000mg/L, C/N/P is 100 ~ 200:5:1, pH value is 6.0 ~ 9.0, in anaerobic biological reactor, temperature controls is 50 ~ 55 DEG C, by the extracellular polymeric of extraction and NiCL
2.6H
2the ratio of O 5:1 ~ 10:1 is in mass ratio mixed and made into mixed liquor A, then is added in anaerobic biological reactor by the proportional concentration of mixed liquor A by 30 ~ 50 μ L/L.
2. the method for promotion algae waste liquid producing methane through anaerobic fermentation according to claim 1, is characterized in that: be muddy water admixture in anaerobic biological reactor, the upwelling that hybrid power is intake from bottom drives.
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| CN103272404B (en) * | 2013-05-22 | 2014-10-29 | 温州大学 | Biological membrane extracellular polymeric substance extraction and grading analysis method of biological membrane reactor |
| CN103304626B (en) * | 2013-06-20 | 2014-12-31 | 中国科学院南京地理与湖泊研究所 | Method for extracting blue-green algae extracellular polymeric substance in grading manner |
| CN103710392B (en) * | 2013-12-24 | 2015-11-18 | 齐鲁工业大学 | A kind of methane conversion promotor and preparation method thereof and application |
| CN107513539A (en) * | 2017-09-07 | 2017-12-26 | 福建农林大学 | A kind of method for promoting anaerobic digestion methane phase using extracellular polymeric |
| US11180707B2 (en) * | 2018-08-03 | 2021-11-23 | Faramaz Fred Faramarzi | System and method for duplicating flammable gas |
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| CN101418316A (en) * | 2008-11-11 | 2009-04-29 | 江南大学 | Method for producing marsh gas through mixed anaerobic fermentation of blue algae and sludge |
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| CN101418316A (en) * | 2008-11-11 | 2009-04-29 | 江南大学 | Method for producing marsh gas through mixed anaerobic fermentation of blue algae and sludge |
Non-Patent Citations (3)
| Title |
|---|
| 吕英.活性污泥胞外聚合物提取方法及其对重金属离子的吸附研究.《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》.2008,第2页第2段和第3页表1,以及51页5.4小结,第20-21页3.1.2.2部分. * |
| 胡庆昊.镍及其螯合物对甲烷发酵的影响及机理研究.《中国博士学位论文全文数据库工程科技Ⅰ辑》.2009,第13-15页2.2.1-2.2.2部分和第17页2.3.2部分,第23页2.4本章小结. * |
| 邹小玲等.不同状态下的同一污泥胞外聚合物提取方法研究.《环境工程学报》.2010,第4卷(第2期),第436-440页. * |
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