CN102994565A - Method for enhancing anaerobic fermentation of algal waste liquid to generate methane - Google Patents
Method for enhancing anaerobic fermentation of algal waste liquid to generate methane Download PDFInfo
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- CN102994565A CN102994565A CN2012105550140A CN201210555014A CN102994565A CN 102994565 A CN102994565 A CN 102994565A CN 2012105550140 A CN2012105550140 A CN 2012105550140A CN 201210555014 A CN201210555014 A CN 201210555014A CN 102994565 A CN102994565 A CN 102994565A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention relates to a method for enhancing anaerobic fermentation of algal waste liquid to generate methane. The method comprises the following steps of: firstly adopting a centrifugation method to extract extracellular polymeric substances of anaerobic sludge; mixing and adding the algal waste liquid and anaerobic sludge into an anaerobic bioreactor; adjusting the nutritive ratio of C/N/P in the anaerobic bioreactor, wherein the inflowing water quality after the nutritive ratio is adjusted is as follows: COD is 20000-25000mg/L, C/N/P is (100-200):5:1, and pH value is 6.0-9.0; and after preheating, preparing the extracted extracellular polymeric substances and NiCL2.6H2O into mixed liquid A and adding the mixed liquid A into the anaerobic bioreactor. The method has the advantages that necessary trace element Ni for growing the methanogen is chelated into a dissolved-state biological promoter for utilization of the methanogen, the chemical precipitation action is overcome, the utilization ratio of the trace element is increased, the gas generation ratio for anaerobic fermentation of the algal waste liquid is increased to the maximum extent, the adding amount of the promoter is less, the cost is low and the operation is simple.
Description
Technical field
The present invention relates to a kind of water technology, particularly a kind of method that promotes algae waste liquid producing methane through anaerobic fermentation.
Background technology
The microorganism amount reproduction such as blue-green algae after seawater or the poisons in freshwater eutrophication, thereby can cause respectively red tide or wawter bloom phenomenon, as in May, 2008 Taihu Lake the big area wawter bloom appears, wawter bloom is often processed in the mode of artificial salvaging prolific algae after occurring, the filtrate organic content that the algae of collecting produces after press filtration is high, need to process and could discharge water body.Producing methane through anaerobic fermentation is a kind of economy of processing this class waste liquid, feasible resource reutilization method.
In algae waste liquid anaerobic fermentation process, the growth of methanogen requires also to need some necessary micro-metalss in suitable scope except the C/N/P nutrition-allocated proportion.There are some researches show that the interpolation of micro-metals Ni can promote the raising of biogas yield.But because the existence of anaerobic reactor medium sulphide content, vitriol, phosphoric acid salt or carbonate causes the chemical precipitation of Ni element easily.Therefore, seek a kind of desirable sequestrant, the Ni element is sequestered in inner complex inside, its form with solubilised state is present in the anaerobic reactor, for the methanogen utilization, just become the key issue that improves algae waste liquid anaerobic fermentation gas production rate.
Summary of the invention
The technical problem to be solved in the present invention is for the deficiencies in the prior art, has proposed a kind of method that has overcome chemical precipitation, improved the promotion algae waste liquid producing methane through anaerobic fermentation of micro-utilization ratio.
The technical problem to be solved in the present invention is achieved through the following technical solutions, and a kind of method that promotes algae waste liquid producing methane through anaerobic fermentation is characterized in:
At first adopt centrifuging to extract the extracellular polymeric EPS of anaerobic sludge, take the anaerobic sludge mixed solution of mass concentration 8 ~ 9g/L, centrifugal 10 ~ 15min under the condition of 3000 ~ 3500r/min, abandoning supernatant is also supplied twice like this with distilled water; Centrifugal 10 ~ 15min again under the condition of 3000 ~ 3500r/min, direct high speed centrifugation 10 ~ 15min under the condition of 10000 ~ 12000r/min after the abandoning supernatant, the supernatant liquor of getting behind the high speed centrifugation can obtain extracellular polymeric EPS behind 0.22 μ m membrane filtration; Organic content is 23% ~ 61% among the extracellular polymeric EPS, protein content is 2.1% ~ 28.3%, saccharan content is 1.0% ~ 23.0%, dna content is 0% ~ 5.2%;
Algae waste liquid and anaerobic sludge are mixed to join among the anaerobic biological reactor UASB, the concentration that makes anaerobic sludge account for total amount of liquid is 8 ~ 9g/L, again the C/N/P nutrition ratio in the anaerobic biological reactor UASB is adjusted, concrete grammar is: use methyl alcohol to change the ratio of organic carbon, use urea to change the ratio of nitrogen, use phosphoric acid salt to change the ratio of phosphorus, adjusting nutrition than rear influent quality is: COD is 20000 ~ 25000mg/L, C/N/P is 100 ~ 200:5:1, pH value is 6.0 ~ 9.0, adopt calrod the method for water inlet preheating to be made temperature is controlled to be 50 ~ 55 ℃ in the anaerobic biological reactor UASB, with extracellular polymeric EPS and the NiCL that extracts
2.6H
2The O in mass ratio ratio of 5:1 ~ 10:1 is mixed and made into mixed liquor A, the proportional concentration of mixed liquor A by 30 ~ 50 μ L/L is added among the anaerobic biological reactor UASB again.
The technical problem to be solved in the present invention can also be come further to realize by the following technical programs, is the muddy water admixture among the anaerobic biological reactor UASB, and hybrid power drives from the upwelling of bottom water inlet.
Beneficial effect of the present invention: key point of the present invention is to extract extracellular polymeric EPS as sequestrant from the anaerobic sludge of sewage treatment process structures anaerobic biological reactor UASB inside, be the solubilised state Biostimulation agent for the methanogen utilization with the methanogen necessary micro-Ni chelating of growing, overcome chemical precipitation, improved micro-utilization ratio.Sequestrant comes from the inner microorganisms of anaerobic reactor, has high-adaptability with reactor system, at utmost improved the factor of created gase of algae waste liquid anaerobically fermenting, factor of created gase has improved 53% ~ 72%, and the promotor addition is small, and cost is low, and is simple to operate, successful can be used for engineering practice.
Embodiment
A kind of method that promotes algae waste liquid producing methane through anaerobic fermentation, algae waste liquid producing methane through anaerobic fermentation process be in essence methanogen in the anaerobic biological reactor to the process of utilizing of organic substrates, and methanogen is present in inside reactor with the form of anaerobic sludge.So, in the chosen process of sequestrant, should from anaerobic sludge, extract, could at utmost adapt to the system environment in the anaerobic reactor.
At first adopt centrifuging to extract the extracellular polymeric EPS of anaerobic sludge, take the anaerobic sludge mixed solution of mass concentration 8 ~ 9g/L, centrifugal 10 ~ 15min under the condition of 3000 ~ 3500r/min, abandoning supernatant is also supplied twice like this with distilled water; Centrifugal 10 ~ 15min again under the condition of 3000 ~ 3500r/min, direct high speed centrifugation 10 ~ 15min under the condition of 10000 ~ 12000r/min after the abandoning supernatant, the supernatant liquor of getting behind the high speed centrifugation can obtain extracellular polymeric EPS behind 0.22 μ m membrane filtration; Organic content is 23% ~ 61% among the extracellular polymeric EPS, protein content is 2.1% ~ 28.3%, saccharan content is 1.0% ~ 23.0%, dna content is 0% ~ 5.2%;
Algae waste liquid and anaerobic sludge are mixed to join among the anaerobic biological reactor UASB, the concentration that makes anaerobic sludge account for total amount of liquid is 8 ~ 9g/L, again the C/N/P nutrition ratio in the anaerobic biological reactor UASB is adjusted, concrete grammar is: use methyl alcohol to change the ratio of organic carbon, use urea to change the ratio of nitrogen, use phosphoric acid salt to change the ratio of phosphorus, adjusting nutrition than rear influent quality is: COD (chemical oxygen demand (COD)) is 20000 ~ 25000mg/L, C/N/P is 100 ~ 200:5:1, pH value is 6.0 ~ 9.0, adopt calrod the method for water inlet preheating to be made temperature is controlled to be 50 ~ 55 ℃ in the anaerobic biological reactor UASB, with extracellular polymeric EPS and the NiCL that extracts
2.6H
2The O in mass ratio ratio of 5:1 ~ 10:1 is mixed and made into mixed liquor A, again the proportional concentration of mixed liquor A by 30 ~ 50 μ L/L is added among the anaerobic biological reactor UASB, be the muddy water admixture among the anaerobic biological reactor UASB, hybrid power drives from the upwelling of bottom water inlet.
Mixed liquor A is added among the anaerobic biological reactor UASB by the proportional concentration of 30 ~ 50 μ L/L, and the meaning is exactly: the ratio of total amount of liquid is 30 ~ 50 μ L/L among mixed liquor A add-on and the anaerobic biological reactor UASB.The amount that namely adds mixed liquor A in the liquid in every L anaerobic biological reactor UASB is 30 ~ 50 μ L.
Described anaerobic sludge is taken from city domestic sewage treatment plant, city domestic sewage after anaerobic biological is processed, the formed throw out of the microorganism that in reactor, grows.
Below just the present invention illustrate, but be not limitation of the present invention.
Case of comparative examples:
The algae waste liquid is 0.32m at the biogas yield that does not add uniform temp under the condition of Biostimulation agent and carry out anaerobically fermenting
3/ kgCOD.
Embodiment 1
Algae waste liquid COD is 21100mg/L, and C/N/P is 180:5:1, and PH is 6.7, and initial anaerobic sludge concentration is 8.2g/L, and temperature is 50 ~ 55 ℃.Extracellular polymeric EPS organic content is 31%, and wherein protein content is 8.1%, and saccharan is that 6.5%, DNA is 0.7%.EPS and NiCL in the mixed liquor A
2.6H
2The mass ratio of O is 10:1, and the concentration that mixed liquor A adds in UASB is 30 μ L/L.Measure under the same terms and measuring method, biogas yield is 0.49 m
3/ kgCOD, comparison has improved 53% as a rule.
Embodiment 2
Algae waste liquid COD is 24300mg/L, and C/N/P is 200:5:1, and PH is 8.2, and initial anaerobic sludge concentration is 8.5g/L, and temperature is 50 ~ 55 ℃.Extracellular polymeric EPS organic content is 61%, and wherein protein content is 10.7%, and saccharan is that 8.2%, DNA is 1.9%.EPS and NiCL in the mixed liquor A
2.6H
2The mass ratio of O is 5:1, and the concentration that mixed liquor A adds in UASB is 50 μ L/L.Measure under the same terms and measuring method, biogas yield is 0.55 m
3/ kgCOD, comparison has improved 72% as a rule.
Claims (2)
1. method that promotes algae waste liquid producing methane through anaerobic fermentation, it is characterized in that: at first adopt centrifuging to extract the extracellular polymeric of anaerobic sludge, take the anaerobic sludge mixed solution of mass concentration 8 ~ 9g/L, centrifugal 10 ~ 15min under the condition of 3000 ~ 3500r/min, abandoning supernatant is also supplied twice like this with distilled water; Centrifugal 10 ~ 15min again under the condition of 3000 ~ 3500r/min, direct high speed centrifugation 10 ~ 15min under the condition of 10000 ~ 12000r/min after the abandoning supernatant, the supernatant liquor of getting behind the high speed centrifugation can obtain extracellular polymeric behind 0.22 μ m membrane filtration; Organic content is 23% ~ 61% in the extracellular polymeric, protein content is 2.1% ~ 28.3%, saccharan content is 1.0% ~ 23.0%, dna content is 0% ~ 5.2%;
Algae waste liquid and anaerobic sludge are mixed to join in the anaerobic biological reactor, the concentration that makes anaerobic sludge account for total amount of liquid is 8 ~ 9g/L, again the C/N/P nutrition ratio in the anaerobic biological reactor is adjusted, concrete grammar is: use methyl alcohol to change the ratio of organic carbon, use urea to change the ratio of nitrogen, use phosphoric acid salt to change the ratio of phosphorus, adjusting nutrition than rear influent quality is: COD is 20000 ~ 25000mg/L, C/N/P is 100 ~ 200:5:1, pH value is 6.0 ~ 9.0, temperature is controlled to be 50 ~ 55 ℃ in the anaerobic biological reactor, with extracellular polymeric and the NiCL that extracts
2.6H
2The O in mass ratio ratio of 5:1 ~ 10:1 is mixed and made into mixed liquor A, the proportional concentration of mixed liquor A by 30 ~ 50 μ L/L is added in the anaerobic biological reactor again.
2. the method for promotion algae waste liquid producing methane through anaerobic fermentation according to claim 1 is characterized in that: be the muddy water admixture in the anaerobic biological reactor, the upwelling drive that hybrid power is intake from the bottom.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103272404A (en) * | 2013-05-22 | 2013-09-04 | 温州大学 | Biological membrane extracellular polymeric substance extraction and grading analysis method of biological membrane reactor |
| CN103304626A (en) * | 2013-06-20 | 2013-09-18 | 中国科学院南京地理与湖泊研究所 | Method for extracting blue-green algae extracellular polymeric substance in grading manner |
| CN103710392A (en) * | 2013-12-24 | 2014-04-09 | 齐鲁工业大学 | Methane conversion accelerator as well as preparation method and application thereof |
| CN107513539A (en) * | 2017-09-07 | 2017-12-26 | 福建农林大学 | A kind of method for promoting anaerobic digestion methane phase using extracellular polymeric |
| CN112996919A (en) * | 2018-08-03 | 2021-06-18 | F·F·弗拉玛兹 | System and method for replicating combustible gases |
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| CN101418316A (en) * | 2008-11-11 | 2009-04-29 | 江南大学 | Method for producing marsh gas through mixed anaerobic fermentation of blue algae and sludge |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103272404A (en) * | 2013-05-22 | 2013-09-04 | 温州大学 | Biological membrane extracellular polymeric substance extraction and grading analysis method of biological membrane reactor |
| CN103304626A (en) * | 2013-06-20 | 2013-09-18 | 中国科学院南京地理与湖泊研究所 | Method for extracting blue-green algae extracellular polymeric substance in grading manner |
| CN103304626B (en) * | 2013-06-20 | 2014-12-31 | 中国科学院南京地理与湖泊研究所 | Method for extracting blue-green algae extracellular polymeric substance in grading manner |
| CN103710392A (en) * | 2013-12-24 | 2014-04-09 | 齐鲁工业大学 | Methane conversion accelerator as well as preparation method and application thereof |
| CN103710392B (en) * | 2013-12-24 | 2015-11-18 | 齐鲁工业大学 | A kind of methane conversion promotor and preparation method thereof and application |
| CN107513539A (en) * | 2017-09-07 | 2017-12-26 | 福建农林大学 | A kind of method for promoting anaerobic digestion methane phase using extracellular polymeric |
| CN112996919A (en) * | 2018-08-03 | 2021-06-18 | F·F·弗拉玛兹 | System and method for replicating combustible gases |
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