CN102960444A - Preparation method of goat milk containing ACE (Angiotensin Converting Enzyme) inhibitory peptide based on Lactobacillus helveticus fermentation - Google Patents
Preparation method of goat milk containing ACE (Angiotensin Converting Enzyme) inhibitory peptide based on Lactobacillus helveticus fermentation Download PDFInfo
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Abstract
The invention discloses a preparation method of goat milk containing ACE (Angiotensin Converting Enzyme) inhibitory peptide based on Lactobacillus helveticus fermentation. The preparation method comprises the following steps of: vaccinating Lactobacillus helveticus in goat milk or goat milk reconstituted milk, and carrying out anaerobic fermentation at a temperature of 30-40 DEG C for 10-15 hours, so as to obtain fermented goat milk; sufficiently mixing 80-90 parts of the fermented goat milk, 5-10 parts of sucrose, 0.15-0.5 parts of a stabilizer and 5-30 parts of water and then cooling to a temperature below 10 DEG C, so as to obtain mixed fermented goat milk; and sealing the mixed fermented goat milk at a temperature of 2-5 DEG C, and maturating for 8-10 hours so as to obtain the goat milk containing the ACE inhibitory peptide. The prepared goat milk has nutritive values of goat yogurt and specific tastes, and further contains the ACE inhibitory peptide with higher inhibition rate, so that not only can the development and the utilization of the goat milk resources be prompted, but also the goat milk has positive effects on hypertension prevention and nutrition and health care of a patient suffering from hypertension.
Description
Technical field
The invention belongs to the goat milk fermentation technical field, relate to a kind of preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation.
Background technology
The World Health Organization (WHO) is defined as hypertension: do not using in the situation of antihypertensive drugs non-on the same day 3 Measure blood pressures, systolic pressure 〉=140mmHg and/or diastolic pressure 〉=90mmHg.National resident 270,000 people's nutrition and investigation of health conditions data according to sanitation Ministry tissue in 2010 show that China 60 years old and the hypertensive illness rate of above crowd are 49%, namely just have 1 people to suffer from hypertension among per approximately 2 people more than 60 years old.In recent years, the children hypertension illness rate also raises to some extent, according to the investigation result of nearly 10 years drug in some provinces, and the children hypertension illness rate, the preschool child is 2 ~ 4%, the school-ager is 4 ~ 9%.Hypertension can cause the multiple complications such as cerebral apoplexy, myocardial infarction, heart failure and CKD, not only disables, fatal rate is high, and seriously consumes medical treatment and social resources, causes heavy burden for family and country.
At present antihypertensive drugs commonly used comprises calcium channel blocker, angiotensin converting enzyme inhibitor (ACEI), ARB (ARB), diuretics, α ARBs and beta-blocker six classes, and the fixed mixing ratio compound preparation that is comprised of said medicine.
Ace inhibitory peptide is the polypeptides matter that the class among the ACEI has antihypertensive function, and it does not have hypotensive activity for normotensive animal or human, have safe, the characteristics that have no side effect.At present, the researcher has isolated the ace inhibitory peptide of multiple natural origin, wherein mostly is to utilize the various food proteins of protease hydrolytic to obtain.There is research to find that some sour milks also have antihypertensive function, and has the scholar therefrom to separate to have obtained ace inhibitory peptide.
Goat milk is nutritious with it, be easy to the plurality of advantages such as absorption is described as elaboration in the milk.Lactobacillus helveticus (Lactobacillus helveticus) is a kind of novel Yoghourt Production bacterial classification with health-care efficacy, its protease hydrolytic vigor is high, can produce multiple biologically active peptide to the human body beneficial by lactoalbumin hydrolysate, the ace inhibitory peptide inhibiting rate of its generation is higher.
Summary of the invention
The problem that the present invention solves is to provide a kind of preparation method of the goat milk that contains ace inhibitory peptide based on the Lactobacillus helveticus fermentation, prepared goat milk had both had nutritive value and the peculiar flavour of acidophilus goat milk, also contain the higher ace inhibitory peptide of inhibiting rate, not only promoted the development and use of goat milk resource, for hypertensive control and hyperpietic's nutrition and health care positive role has been arranged also.
The present invention is achieved through the following technical solutions:
A kind of preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation may further comprise the steps:
1) Lactobacillus helveticus is inoculated in goat milk or the goat milk reconstituted milk, at 30～40 ℃ of lower constant temperature anaerobic fermentation 10～15h, obtains fermented sheep milk;
2) according to the mass fraction, get 80～90 parts of the fermented sheep milks that step 1) obtains, and be cooled to below 10 ℃ after 5～10 parts sucrose, 0.15～0.5 part stabilizing agent and 5～30 water fully mix, obtain mixed culture fermentation goat milk;
3) with mixed culture fermentation goat milk at 2～5 ℃ of condition lower seals, after-ripening 8～10h obtains containing the goat milk of ace inhibitory peptide.
Described Lactobacillus helveticus also carried out following activation before inoculation:
1) the freeze-dried vaccine powder with Lactobacillus helveticus is inoculated in the anaerobism pipe that sterilization goat milk reconstituted milk is housed, and fully cultivates 10～14h 37 ℃ of anaerobism behind the mixing, finishes for the first time activation;
2) mark meter is by volume got 3～7% bacterium liquid of activation for the first time, is inoculated in the anaerobism pipe that sterilization goat milk reconstituted milk is housed, and fully cultivates 10～14h 37 ℃ of anaerobism behind the mixing, finishes for the second time activation;
3) mark meter is by volume got 3～7% bacterium liquid of activation for the second time, is inoculated in the anaerobism pipe that sterilization goat milk reconstituted milk is housed, and fully cultivates 10～14h 37 ℃ of anaerobism behind the mixing, finishes for the third time activation;
After finishing for the third time activation, by volume the mark meter is got 3～7% bacterium liquid that activate for the second time and is inoculated in goat milk or the goat milk reconstituted milk.
To be goat milk powder and water be dissolved in the water with the mass ratio of 10～20:100 described goat milk reconstituted milk.
Also added casein in described goat milk or the goat milk reconstituted milk, with the coagulability of raising fermented sheep milk and the inhibiting rate of ace inhibitory peptide; By quality ratio, the casein addition is 0.5 ~ 0.9%.
Described stabilizing agent is one or both in pectin, sodium carboxymethylcellulose, the propylene glycol alginate.
The viable count of Lactobacillus helveticus is greater than 10 in the described goat milk that contains ace inhibitory peptide
8Cfu/mL.
The ace inhibitory peptide inhibiting rate is 65～90% in the described goat milk that contains ace inhibitory peptide.
The goat milk that contains ace inhibitory peptide of described preparation is used in the drink of preparation preventing hypertension or hypertension auxiliary curing.
Compared with prior art, the present invention has following useful technique effect:
The preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation provided by the invention, employing prepares the goat milk drink in the mode of Lactobacillus helveticus single strain fermentation, method is simple, not only removed the smell of mutton in the goat milk, also kept the nutriment that enriches in the goat milk, curdled milk is fine and smooth, is easy to absorb; The lactobacillus bulgaricus viable count is greater than 10 in the prepared fermented sheep milk
8Cfu/mL can provide to comprise a series of prebiotic effects such as regulating gastrointestinal bacterial flora balance, enhancing immunity, reduction cholesterol.
Further, also add during the fermentation casein, improved the inhibiting rate of ace inhibitory peptide in the fermented sheep milk, also increased protein and amino acid whose content in the acidified milk; And in order to guarantee that the ace inhibitory peptide that produces in the goat milk sweat has higher inhibiting rate, after fermentation ends, also added sucrose and stabilizing agent.
The preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation provided by the invention, the ace inhibitory peptide inhibiting rate can reach 75～90% in the prepared fermented sheep milk, adhere to long-term drinking, the effectively morbidity of preventing hypertension, the auxiliary curing that also can be used for simultaneously the hyperpietic, can use with the drink for preparing preventing hypertension or hypertension auxiliary curing in.
The specific embodiment
The present invention is described in further detail below in conjunction with specific embodiment, and the explanation of the invention is not limited.
Embodiment 1
Preparation method based on the goat milk that contains ace inhibitory peptide of Lactobacillus helveticus fermentation may further comprise the steps:
1) the freeze-dried vaccine powder of getting the 0.4g Lactobacillus helveticus is inoculated in the anaerobism pipe that 10mL sterilization goat milk reconstituted milk is housed, and fully cultivates 12h at 37 ℃ behind the mixing, finishes for the first time activation; Get the bacterium liquid of the 5% above-mentioned activation first time, undertaken for the second time, activate for the third time by the aforesaid operations method, co-activating 3 times namely obtains the bacterial classification that activates.
2) be that 1% casein solution is added in the bright goat milk of 90mL and mixed with 10mL concentration, behind the filtration, purification at 90 ℃ of sterilization 15min, be cooled to 40 ± 3 ℃ after, the Lactobacillus helveticus that activates of access 5mL, at 37 ℃ of constant temperature anaerobic fermentation 12h, obtain fermented sheep milk.
3) in mass fraction, with 5.7 parts sucrose, 0.3 part pectin with 10 parts water-soluble solution, behind 65 ℃ of pasteurize 20min, be cooled to 40 ± 3 ℃, join again in 84 parts the fermented sheep milk, mix rear being cooled to rapidly below 10 ℃, at 4 ℃ of refrigerator after-ripening 8～10h, namely make product again.
Switzerland's living preparation of lactobacillus number is 5.87 * 10 in the products obtained therefrom
8Cfu/mL, ace inhibitory peptide inhibiting rate wherein is 88.36%.
The detection method of ace inhibitory peptide inhibiting rate is: ultraviolet spectrophotometry
With the 0.1mol/L borate buffer solution (pH8.3) that contains 0.3mol/L NaCl with HHL(Hip-His-Leu) be made into the solution of 5mmol/L.In the 10mL test tube, add respectively the 5mmol/L Hip-His-Leu solution of 200 μ L and the acidified milk supernatant of 80 μ L, behind 37 ℃ of lower insulation 5min, adding 20 μ L ACE solution (is dissolved in the distilled water again, vigor is 0.1U/mL), react at 37 ℃ of lower insulation 30min behind the mixing, add afterwards 250 μ L 1mol/L HCl cessation reactions, add again the 1.5mL ethyl acetate, behind 15s vibration mixing, leave standstill 5min, with the ethyl acetate layer of pipette, extract 1.0mL, in 120 ℃ of oven dry 30min, add again 1.0mL distilled water, measure absorbance behind the mixing at the 228nm place.Under these conditions, the ACE of inhibition 50% active (IC50) is an active unit of sample.
ACE inhibiting rate=[(B-A)/(B-C)] * 100%
In the formula:
A---contain the absorbance of the acidified milk whey polypeptide solution of distilled water (or be dissolved in) and ACE solution;
B---do not contain the acidified milk whey polypeptide solution of distilled water (or be dissolved in) sample, but contain the absorbance of ACE solution;
C---for containing skimmed milk whey (or distilled water), but do not contain the absorbance of ACE solution.
Embodiment 2
Preparation method based on the goat milk that contains ace inhibitory peptide of Lactobacillus helveticus fermentation may further comprise the steps:
1) the freeze-dried vaccine powder of getting the 0.4g Lactobacillus helveticus is inoculated in the anaerobism pipe that 10mL sterilization goat milk reconstituted milk is housed, and fully cultivates 12h at 37 ℃ behind the mixing, finishes for the first time activation; Get the bacterial classification of the 5% above-mentioned activation first time, carry out the activation second time by the aforesaid operations method, co-activating 3 times namely obtains the bacterial classification that activates.
2) be that 1% casein solution is added in the 80mL water and mixed with 14g goat milk powder and 20mL concentration, at 90 ℃ of sterilization 15min, be cooled to 40 ± 3 ℃ after, the Lactobacillus helveticus that access 7mL activates at 40 ℃ of constant temperature anaerobic fermentation 10h, obtains fermented sheep milk.
3) in mass fraction, with 7 parts sucrose, 0.2 part sodium carboxymethylcellulose with 7.8 parts water-soluble solution, behind 65 ℃ of pasteurize 20min, be cooled to 40 ± 3 ℃, join again in 85 parts the fermented sheep milk, mix rear being cooled to rapidly below 10 ℃, at 4 ℃ of refrigerator after-ripening 8 ~ 10h, namely make product again.
Switzerland's living preparation of lactobacillus number is 4.75 * 10 in the products obtained therefrom
8Cfu/mL, ace inhibitory peptide inhibiting rate wherein is 85.73%.
Embodiment 3
Preparation method based on the goat milk that contains ace inhibitory peptide of Lactobacillus helveticus fermentation may further comprise the steps:
1) the freeze-dried vaccine powder of getting the 0.4g Lactobacillus helveticus is inoculated in the anaerobism pipe that 10mL sterilization goat milk reconstituted milk is housed, and fully cultivates 12h at 37 ℃ behind the mixing, finishes for the first time activation; Get the bacterial classification of the 5% above-mentioned activation first time, carry out the activation second time by the aforesaid operations method, co-activating 3 times namely obtains the bacterial classification that activates.
2) be that 1% casein solution is added in the 50mL water and mixed with 14g goat milk powder and 50mL concentration, at 90 ℃ of sterilization 15min, be cooled to 40 ± 3 ℃ after, the Lactobacillus helveticus that access 7mL activates at 35 ℃ of constant temperature anaerobic fermentation 14h, obtains fermented sheep milk.
3) in mass fraction, with 5 parts sucrose, 0.3 part propylene glycol alginate with 6.7 parts water-soluble solution, behind 65 ℃ of pasteurize 20min, be cooled to 40 ± 3 ℃, join again in 88 parts the fermented sheep milk, mix rear being cooled to rapidly below 10 ℃, at 4 ℃ of refrigerator after-ripening 8 ~ 10h, namely make product again.
Switzerland's living preparation of lactobacillus number is 4.68 * 10 in the products obtained therefrom
8Cfu/mL, ace inhibitory peptide inhibiting rate wherein is 83.17%.
Embodiment 4
Preparation method based on the goat milk that contains ace inhibitory peptide of Lactobacillus helveticus fermentation may further comprise the steps:
1) the freeze-dried vaccine powder of getting the 0.4g Lactobacillus helveticus is inoculated in the anaerobism pipe that 10mL sterilization goat milk reconstituted milk is housed, and fully cultivates 12h at 37 ℃ behind the mixing, finishes for the first time activation; Get the bacterium liquid of the 5% above-mentioned activation first time, undertaken for the second time, activate for the third time by the aforesaid operations method, co-activating 3 times namely obtains the bacterial classification that activates.
2) be that 1% casein solution is added in the bright goat milk of 80mL and mixed with 20mL concentration, behind the filtration, purification at 90 ℃ of sterilization 15min, be cooled to 40 ± 3 ℃ after, the Lactobacillus helveticus that activates of access 7mL, at 37 ℃ of constant temperature anaerobic fermentation 11h, obtain fermented sheep milk.
3) in mass fraction, with 7 parts sucrose, 0.2 part sodium carboxymethylcellulose with 10.8 parts water-soluble solution, behind 65 ℃ of pasteurize 20min, be cooled to 40 ± 3 ℃, join again in 82 parts the fermented sheep milk, mix rear being cooled to rapidly below 10 ℃, at 4 ℃ of refrigerator after-ripening 8 ~ 10h, namely make product again.
Switzerland's living preparation of lactobacillus number is 5.66 * 10 in the products obtained therefrom
8Cfu/mL, ace inhibitory peptide inhibiting rate wherein is 85.44%.
Embodiment 5
Preparation method based on the goat milk that contains ace inhibitory peptide of Lactobacillus helveticus fermentation may further comprise the steps:
1) the freeze-dried vaccine powder of getting the 0.4g Lactobacillus helveticus is inoculated in the anaerobism pipe that 10mL sterilization goat milk reconstituted milk is housed, and fully cultivates 12h at 37 ℃ behind the mixing, finishes for the first time activation; Get the bacterium liquid of the 5% above-mentioned activation first time, undertaken for the second time, activate for the third time by the aforesaid operations method, co-activating 3 times namely obtains the bacterial classification that activates.
2) be that 1% casein solution is added in the bright goat milk of 80mL and mixed with 20mL concentration, behind the filtration, purification at 90 ℃ of sterilization 15min, be cooled to 40 ± 3 ℃ after, the Lactobacillus helveticus that activates of access 7mL, at 35 ℃ of constant temperature anaerobic fermentation 14h, obtain fermented sheep milk.
3) in mass fraction, with 5 parts sucrose, 0.1 part sodium carboxymethylcellulose, 0.1 part pectin with 7.8 parts water-soluble solution, behind 65 ℃ of pasteurize 20min, be cooled to 40 ± 3 ℃, join again in 87 parts the fermented sheep milk, mix rear being cooled to rapidly below 10 ℃, at 4 ℃ of refrigerator after-ripening 8 ~ 10h, namely make product again.
Switzerland's living preparation of lactobacillus number is 4.42 * 10 in the products obtained therefrom
8Cfu/mL, ace inhibitory peptide inhibiting rate wherein is 79.80%.
Prepared fermented sheep milk is adhered to long-term drinking, the effectively morbidity of preventing hypertension, and the while also can be used for hyperpietic's auxiliary curing, can be applicable to prepare in the drink of preventing hypertension or hypertension auxiliary curing.
Claims (8)
1. the preparation method based on the goat milk that contains ace inhibitory peptide of Lactobacillus helveticus fermentation is characterized in that, may further comprise the steps:
1) Lactobacillus helveticus is inoculated in goat milk or the goat milk reconstituted milk, at 30～40 ℃ of lower constant temperature anaerobic fermentation 10～15h, obtains fermented sheep milk;
2) according to the mass fraction, get 80～90 parts of the fermented sheep milks that step 1) obtains, and be cooled to below 10 ℃ after 5～10 parts sucrose, 0.15～0.5 part stabilizing agent and 5～30 water fully mix, obtain mixed culture fermentation goat milk;
3) with mixed culture fermentation goat milk at 2～5 ℃ of condition lower seals, after-ripening 8～10h obtains containing the goat milk of ace inhibitory peptide.
2. the preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation as claimed in claim 1 is characterized in that described Lactobacillus helveticus also carried out following activation before inoculation:
1) the freeze-dried vaccine powder with Lactobacillus helveticus is inoculated in the anaerobism pipe that sterilization goat milk reconstituted milk is housed, and fully cultivates 10～14h 37 ℃ of anaerobism behind the mixing, finishes for the first time activation;
2) mark meter is by volume got 3～7% bacterium liquid of activation for the first time, is inoculated in the anaerobism pipe that sterilization goat milk reconstituted milk is housed, and fully cultivates 10～14h 37 ℃ of anaerobism behind the mixing, finishes for the second time activation;
3) mark meter is by volume got 3～7% bacterium liquid of activation for the second time, is inoculated in the anaerobism pipe that sterilization goat milk reconstituted milk is housed, and fully cultivates 10～14h 37 ℃ of anaerobism behind the mixing, finishes for the third time activation;
After finishing for the third time activation, by volume the mark meter is got 3～7% bacterium liquid that activate for the second time and is inoculated in goat milk or the goat milk reconstituted milk.
3. the preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation as claimed in claim 1 is characterized in that, to be goat milk powder and water be dissolved in the water with the mass ratio of 10～20:100 described goat milk reconstituted milk.
4. the preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation as claimed in claim 1 is characterized in that, has also added casein in described goat milk or the goat milk reconstituted milk, to improve the inhibiting rate of ace inhibitory peptide; By quality ratio, the casein addition is 0.1～0.5%.
5. the preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation as claimed in claim 1 is characterized in that described stabilizing agent is one or both in pectin, sodium carboxymethylcellulose, the propylene glycol alginate.
6. the preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation as claimed in claim 1 is characterized in that, the viable count of Lactobacillus helveticus is greater than 10 in the described goat milk that contains ace inhibitory peptide
8Cfu/mL.
7. the preparation method of the goat milk that contains ace inhibitory peptide based on Lactobacillus helveticus fermentation as claimed in claim 1 is characterized in that, the inhibiting rate of ace inhibitory peptide is 65～90% in the described goat milk that contains ace inhibitory peptide.
8. the goat milk that contains ace inhibitory peptide of the described preparation of claim 1 is used in the drink of preparation preventing hypertension or hypertension auxiliary curing.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222505A (en) * | 2013-04-24 | 2013-07-31 | 陕西科技大学 | Preparation method of lactobacillus plantarum fermented goat milk containing ACE inhibitory peptide |
| CN103637334A (en) * | 2013-11-20 | 2014-03-19 | 永城市弥诺食品有限公司 | Wheat germ vegetable protein fermentation peptide beverage and preparation method thereof |
| CN104450574A (en) * | 2014-12-10 | 2015-03-25 | 生合生物科技（南京）有限公司 | Lactobacillus helveticus LH43 and method for preparing lactobacillus beverage by lactobacillus helveticus LH43 |
| CN105685223A (en) * | 2016-02-01 | 2016-06-22 | 陕西科技大学 | Preparation method of antioxidant lactobacillus acidophilus fermented goat milk |
| CN107858388A (en) * | 2017-10-17 | 2018-03-30 | 陕西科技大学 | A kind of method that sheep breast Antihypertensive Peptides are prepared using probiotic lactobacilli protease |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279712A (en) * | 1997-09-26 | 2001-01-10 | 卡尔皮斯株式会社 | Lactobacillus helveticus bacterium having high capability of producing tripentide, fermented milk product, and preparing process thereof |
| WO2004098309A1 (en) * | 2003-05-05 | 2004-11-18 | Unilever N.V. | Hydrolysed casein product comprising tripeptides ipp and/ or vpp |
| CN1735347A (en) * | 2003-01-06 | 2006-02-15 | 荷兰联合利华有限公司 | Fermented milk product comprising tripeptide VPP and/or IPP |
| CN102329763A (en) * | 2011-11-01 | 2012-01-25 | 内蒙古农业大学 | Lactobacillus helveticus H9 and application of Lactobacillus helveticus H9 to preparation of fermented milk |
-
2012
- 2012-11-19 CN CN2012104707934A patent/CN102960444A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279712A (en) * | 1997-09-26 | 2001-01-10 | 卡尔皮斯株式会社 | Lactobacillus helveticus bacterium having high capability of producing tripentide, fermented milk product, and preparing process thereof |
| CN1735347A (en) * | 2003-01-06 | 2006-02-15 | 荷兰联合利华有限公司 | Fermented milk product comprising tripeptide VPP and/or IPP |
| WO2004098309A1 (en) * | 2003-05-05 | 2004-11-18 | Unilever N.V. | Hydrolysed casein product comprising tripeptides ipp and/ or vpp |
| CN102329763A (en) * | 2011-11-01 | 2012-01-25 | 内蒙古农业大学 | Lactobacillus helveticus H9 and application of Lactobacillus helveticus H9 to preparation of fermented milk |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222505A (en) * | 2013-04-24 | 2013-07-31 | 陕西科技大学 | Preparation method of lactobacillus plantarum fermented goat milk containing ACE inhibitory peptide |
| CN103637334A (en) * | 2013-11-20 | 2014-03-19 | 永城市弥诺食品有限公司 | Wheat germ vegetable protein fermentation peptide beverage and preparation method thereof |
| CN104450574A (en) * | 2014-12-10 | 2015-03-25 | 生合生物科技（南京）有限公司 | Lactobacillus helveticus LH43 and method for preparing lactobacillus beverage by lactobacillus helveticus LH43 |
| CN105685223A (en) * | 2016-02-01 | 2016-06-22 | 陕西科技大学 | Preparation method of antioxidant lactobacillus acidophilus fermented goat milk |
| CN107858388A (en) * | 2017-10-17 | 2018-03-30 | 陕西科技大学 | A kind of method that sheep breast Antihypertensive Peptides are prepared using probiotic lactobacilli protease |
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Application publication date: 20130313 |