CN102960177A - Method of shortening seed breeding cycle of nicotiana tabacum mutant strain YK01 - Google Patents
Method of shortening seed breeding cycle of nicotiana tabacum mutant strain YK01 Download PDFInfo
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Abstract
The invention discloses a method of shortening seed breeding cycle of a nicotiana tabacum mutant strain YK01, which is characterized by comprising a seedling raising procedure, a transplant procedure, a vegetative period management procedure, a grafting procedure, a reproductive stage management procedure, and a seed recovery procedure. The method specifically comprises the following steps: the growing environment temperature for seedling raising being 5 to 30 DEG C, the oxygen content being 2-21%, the humidity being 50-100%, and the illumination intensity being 1000 to 100, 000 LX; transplanting the cultured nicotiana tabacum mutant strain YK01 strong seedlings to the area at the elevation of 1400-2500 m; after transplanting, the temperature being 5-35 DEG C, the oxygen content being 5-21%, the humidity being 50-90%, and the illumination intensity being 1000-120, 000 LX; after the vegetative period, grafting the seedlings to the area at the elevation of 400-1500 m; after grafting, the growing environment temperature being 5-35 DEG C, the oxygen content being 5-21%, the humidity being 50-90%, and the illumination intensity being 1000-120,000 LX; spraying one thousandth to one hundredth of leaf fertilizer for 0 to 5 times in the florescence; and recovering the mature seeds. The method is simple and convenient to operate, can effectively shorten the seed breeding cycle of the nicotiana tabacum mutant strain YK01, and improves the quality and the yield of the seeds.
Description
Technical field
The invention belongs to technical field of tobacco, further belong to tobacco seed raising technology field, be specifically related to a kind of safflower method in large gold dollar variation strain YK01 seminal propagation cycle that shortens.
Background technology
In September, 2006, the scientific and technical personnel of Kunming tobacco company find a huge variation strain first in the large gold dollar of flue-cured tobacco recommended variety safflower, under local natural growthing condition, surpassing 55d, the individual plant trunk number of sheets its vegetative growth phase surpasses 80, stem girth and reaches about 20cm, transplant and surpass 120d to squaring period, surpass 140d to center flower open season.
The kinds such as the large gold dollar of current our province flue-cured tobacco safflower, K326, cloud and mist 87 are many after the transplanting about 50d grow normal cigarette strain squaring period, and flowering stage is many after the transplanting about 60d.
During 2007~2011 years, by for many years, multi-point shows (seeing Table 1) to the experimental study of red large huge variant: this variant ties up between similar ecological environmental condition succeeding year and all shows basically identical result, squaring period is after the transplanting about 125d the earliest, and flowering stage is after the transplanting about 150d the earliest; But differ greatly between the different location, this may be this variant be converted into from nourishing and growing time of reproductive growth and the ecological conditions such as height above sea level, illumination, heat and transplanting time closely related due to, although show the prematurity characteristic, be not to belong to conventional variety so-called " early blossoming " category yet.Be problems such as solving above-mentioned growth cycle and grow, yield poorly, develop the method for planting that a kind ofly can shorten the seminal propagation cycle, improve seed quality and output and be very important.
Table 1. " red large huge variation strain " for many years, the flowering habit investigation result of buddingging of multiple spot
Summary of the invention
The object of the present invention is to provide a kind of method that shortens the red large variation strain YK01 seminal propagation cycle.
The object of the present invention is achieved like this, comprises the period management of growing seedlings, transplant, nourish and grow, transplanting, reproductive growth period management, seed collection operation, specifically comprises:
A, grow seedlings: the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX;
B, transplanting: the red large variation strain YK01 kind strain that will cultivate is transplanted in the zone of height above sea level 1400~2500m strong sprout, and transplanting time is in 1~June 1 March;
C, the period management of nourishing and growing: the seeding row spacing of red large variation strain YK01 kind strain is 0.5 ~ 1.0m * 1.0 ~ 2.0m after transplanting, the amount of mu applied nitrogen is purity nitrogen 2.0 ~ 7.0Kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5 ~ 2:2 ~ 4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX;
D, transplanting: be transplanted to the zone of height above sea level 400~1500m when nourishing and growing end cycle red large variation strain YK01 kind strain growth 3~5 months;
E, reproductive growth period management: the red large variation strain YK01 kind strain seeding row spacing after the transplanting is 0.5 ~ 1.0m * 1.0 ~ 2.0m, mu is executed well-rotted farmyard manure 0~1000kg, the amount of mu applied nitrogen is purity nitrogen 0.0 ~ 7.0 Kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5 ~ 2:2 ~ 4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX;
F, seed collection: spray 1 ‰~1% foliage fertilizer 0~5 time flowering stage in kind of a strain, behind seed maturity, gather.
The present invention's breeding cycle that has shortened red large variation strain TK01 seed easy and simple to handle, effective, and improved seed quality and output.
Description of drawings
Fig. 1 is workflow diagram of the present invention.
Embodiment
The present invention is further illustrated below in conjunction with drawings and Examples, but never in any form the present invention is limited, and any conversion or replacement based on training centre of the present invention is done all belong to protection scope of the present invention.
The method in red large variation strain YK01 seminal propagation cycle of shortening of the present invention comprises the period management of growing seedlings, transplant, nourish and grow, transplanting, reproductive growth period management, seed collection operation, specifically comprises:
A, grow seedlings: the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX;
B, transplanting: the red large variation strain YK01 kind strain that will cultivate is transplanted in the zone of height above sea level 1400~2500m strong sprout, and transplanting time is in 1~June 1 March;
C, the period management of nourishing and growing: the seeding row spacing of red large variation strain YK01 kind strain is 0.5 ~ 1.0m * 1.0 ~ 2.0m after transplanting, the amount of mu applied nitrogen is purity nitrogen 2.0 ~ 7.0Kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5 ~ 2:2 ~ 4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX;
D, transplanting: be transplanted to the zone of height above sea level 400~1500m when nourishing and growing end cycle red large variation strain YK01 kind strain growth 3~5 months;
E, reproductive growth period management: the red large variation strain YK01 kind strain seeding row spacing after the transplanting is 0.5 ~ 1.0m * 1.0 ~ 2.0m, mu is executed well-rotted farmyard manure 0~1000kg, the amount of mu applied nitrogen is purity nitrogen 0.0 ~ 7.0 Kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5 ~ 2:2 ~ 4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX;
F, seed collection: spray 1 ‰~1% foliage fertilizer 0~5 time flowering stage in kind of a strain, behind seed maturity, gather.
Described transplanting is the zone of kind of strain being transplanted to height above sea level 1500~2500m.
Described transplanting is the zone of kind of strain being transplanted to height above sea level 1700~2500m.
Described transplanting is the zone of kind of strain being transplanted to height above sea level 2000~2500m.
Nitrogenous fertilizer in described C, the E step is one or more in farmyard manure, inorganic fertilizer, fertilizer or the bio-fertilizer.
Described transplanting is the zone of kind of strain being transplanted to height above sea level 400~1400m.
Described transplanting is the zone of kind of strain being transplanted to height above sea level 400~1200m.
Described transplanting is the zone of kind of strain being transplanted to height above sea level 400~1000m.
Well-rotted farmyard manure in the described E step is night soil, pig, ox, sheep, horsehit urine, and bird fecaluria and barnyard manure, compost, biogas manure, smoked soil, a heatable brick bed are native, in the ash one or more.
In the described F step foliage fertilizer be high potassium foliage fertilizer.
The present invention's breeding cycle that has shortened red large variation strain YK01 seed easy and simple to handle, effective, and improved seed quality and output.
Embodiment 1
Cultivate red large variation strain YK01 kind strain strong sprout, the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX; Strong sprout is transplanted to height above sea level 1700m zone in the red large variation strain YK01 kind strain of 500 strains, and red large variation strain YK01 kind strain seeding row spacing is 0.5 m * 2.0m, and the amount of mu applied nitrogen is purity nitrogen 2.0k
g, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5:2, and the growing environment temperature is controlled at 5 ~ 35 ℃, and oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Red large variation strain YK01 kind strain growth 3 ~ 5 months when nourishing and growing end cycle, select 50 strains to be transplanted to the zone of height above sea level 1400m, red large variation strain YK01 kind strain seeding row spacing is 0.5m * 2.0m, the amount of mu applied nitrogen is purity nitrogen 2.0 Kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:1:22, and the growing environment temperature is controlled at 5 ~ 35 ℃, and oxygen content is controlled at 5 ~ 21%, humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Spray 1 ‰~1% blade face potash fertilizer 1 time flowering stage in kind of a strain, gather behind seed maturity, naked kind of 0.3Kg gathers.
Embodiment 2
Cultivate red large variation strain YK01 kind strain strong sprout, the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX; Height above sea level 1800m zone is transplanted in the red large variation strain YK01 kind strain of 500 strains strong sprout, red large variation strain YK01 kind strain seeding row spacing is 1.0m * 2.0m, the amount of mu applied nitrogen is purity nitrogen 7.0kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:2:4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Red large variation strain YK01 kind strain growth 3 ~ 5 months when nourishing and growing end cycle, select 50 strains to be transplanted to the zone of height above sea level 1200m, red large variation strain YK01 kind strain seeding row spacing is 1.0m * 2.0m, mu is executed well-rotted farmyard manure 1000kg, and the amount of mu applied nitrogen is purity nitrogen 5.0kg, and nitrogen phosphorus potassium three essential fertilizer ratio is 1:2:4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Spray 1 ‰ high potassium foliage fertilizers 5 times flowering stage in kind of a strain, gather behind seed maturity, naked kind of 0.35Kg gathers.
Embodiment 3
Cultivate red large variation strain YK01 kind strain strong sprout, the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX; Height above sea level 2300m zone is transplanted in the red large variation strain YK01 kind strain of 500 strains strong sprout, red large variation strain YK01 kind strain seeding row spacing is 1.0m * 1.0 m, the amount of mu applied nitrogen is purity nitrogen 5.0kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:2:2, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Red large variation strain YK01 kind strain growth 3 ~ 5 months when nourishing and growing end cycle, select 50 strains to be transplanted to the zone of height above sea level 800m, red large variation strain YK01 kind strain seeding row spacing is 1.0m * 1.0m, mu is executed well-rotted farmyard manure 800kg, and the amount of mu applied nitrogen is purity nitrogen 4.0kg, and nitrogen phosphorus potassium three essential fertilizer ratio is 1:2:2, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Spray 1% high potassium foliage fertilizer 5 times flowering stage in kind of a strain, gather behind seed maturity, naked kind 0.4 Kg gathers.
Embodiment 4
Cultivate red large variation strain YK01 kind strain strong sprout, the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX; Height above sea level 2000m zone is transplanted in the red large variation strain YK01 kind strain of 500 strains strong sprout, red large variation strain YK01 kind strain seeding row spacing is 0.8m * 1.5m, the amount of mu applied nitrogen is purity nitrogen 4.0kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5:4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Red large variation strain YK01 kind strain growth 3 ~ 5 months when nourishing and growing end cycle, select 50 strains to be transplanted to the zone of height above sea level 1000m, red large variation strain YK01 kind strain seeding row spacing is 0.8m * 1.5m, mu is executed well-rotted farmyard manure 300kg, and the amount of mu applied nitrogen is purity nitrogen 4.5kg, and nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5:4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Spray 2 ‰ high potassium foliage fertilizers 3 times flowering stage in kind of a strain, gather behind seed maturity, naked kind of 0.45Kg gathers.
Embodiment 5
Cultivate red large variation strain YK01 kind strain strong sprout, the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX; Height above sea level 2000m zone is transplanted in the red large variation strain YK01 kind strain of 500 strains strong sprout, red large variation strain YK01 kind strain seeding row spacing is 1.0m * 1.5m, the amount of mu applied nitrogen is purity nitrogen 6.0kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:2:2, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Red large variation strain YK01 kind strain growth 3 ~ 5 months when nourishing and growing end cycle, select 50 strains to be transplanted to the zone of height above sea level 1300m, red large variation strain YK01 kind strain seeding row spacing is 1.0m * 1.5m, mu is executed well-rotted farmyard manure 100kg, and the amount of mu applied nitrogen is purity nitrogen 6.0kg, and nitrogen phosphorus potassium three essential fertilizer ratio is 1:2:2, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX; Spray 1% high potassium foliage fertilizer 5 times flowering stage in kind of a strain, gather behind seed maturity, naked kind of 0.4Kg gathers.
Test example 1
1. materials and methods
1.1 test material
2011, (1300 meters of height above sea level) and white car rib (2000 meters of height above sea level) were research object with red large huge variant in residential district, township, Zhushan County, Yiliang County, carry out the technical research in its seminal propagation cycle of shortening.
1.2 test is processed
A: at the white car rib point of 2000 meters of height above sea level, select 50 kind strains not take any Insulation;
B: at the white car rib point of 2000 meters of height above sea level, in selecting 50 kind strains to build heat preservation greenhouse by the end of August;
C: at the white car rib point of 2000 meters of height above sea level, select 50 kind strains to be transplanted to the residential district point of 1300 meters of height above sea level, build simultaneously roof insulating;
D: at the residential district of 1300 meters of height above sea level point, select 50 strains to follow the tracks of accent and examine, do not take any Insulation.
1.3 test method
At the residential district of 1300 meters of height above sea level point, unified red large huge variation strain (hereinafter to be referred as the YK01) strong sprout of growing seedlings.Concentrate 2000 cigarette strains of plantation at two height above sea level points respectively, comparative observation blooming between them, solid situation.
1.4 control measures
Unified seeding row spacing, unified fertilizing amount, unified transplanting time.Other cultivation step carries out with reference to local High Quality Tobacco production management.
2. results and analysis
Found out that by table 2 two height above sea level points are compared, be very beneficial for shortening nourishing and growing the cycle of YK01 at high height above sea level point, but owing to the later stage is affected obviously by low temperature, frost etc., very unfavorable to the sexual propagation of seed.Show that thus transplanting to prosperous long-term temperature may be to affect the budding key factor of flowering habit of YK01.And entering the reproductive growth after date, by creating the environment of a relatively-high temperature or high effective accumulated temperature, it is relatively concentrated just to guarantee to budding, bloom, thereby can obtain the better result that seed maturity is fast, seed production is high.
Table 2. red large huge variation strain seed sexual reproduction technique research in 2011
Process | Transplanting time (month. day) | Build the roof insulating phase | Transplanting stage | Early squaring (month. day) | Squaring period (month. day) | Initial bloom stage (month. day) | Full-bloom stage (month. day) | Seed maturity | Amount to per mu yield/kg |
A | 5.4 | ---- | ---- | 9.1 | 9.25 | 10.3 | 10.15 | Next year 1.15 | 0.5 |
B | 5.4 | 8.25 | ---- | 9.1 | 9.15 | 10.1 | 10.12 | 12.25 | 3.0 |
C | 5.4 | 8.24 | 8.25 | 9.1 | 9.15 | 10.1 | 10.8 | 11.25 | 8.0 |
D | 5.4 | ---- | ---- | Next year 1.20 | Next year 2.10 | Next year 2.15 | Next year 2.10 | Next year 2.15 | 0.5 |
Remarks: process A owing to affected obviously by winter frost, process strain number that D blooms owing to buddingging seldom, thereby cause the seed production of two processing all very low.
3. conclusion
For the long tobacco of reproductive stage, under stereo-climate of Yunnan province, spend surpassing the 1700m zone at height above sea level its vegetative growth phase, reproductive stage is lower than the 1400m zone at height above sea level spends, and can effectively shorten its seed sexual propagation cycle and increase seed production.
Claims (10)
1. a method that shortens the red large variation strain YK01 seminal propagation cycle is characterized in that comprising grow seedlings, transplant, nourish and grow period management, transplanting, reproductive growth period management, seed collection operation, specifically comprises:
A, grow seedlings: the growing environment temperature is controlled at 5 ~ 30 ℃, and oxygen content is controlled at 2 ~ 21%, and humidity is controlled at 50 ~ 100%, and intensity of illumination is controlled at 1000 ~ 100000LX;
B, transplanting: the red large variation strain YK01 kind strain that will cultivate is transplanted in the zone of height above sea level 1400~2500m strong sprout, and transplanting time is in 1~June 1 March;
C, the period management of nourishing and growing: the seeding row spacing of red large variation strain YK01 kind strain is 0.5 ~ 1.0m * 1.0 ~ 2.0m after transplanting, the amount of mu applied nitrogen is purity nitrogen 2.0 ~ 7.0Kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5 ~ 2:2 ~ 4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX;
D, transplanting: be transplanted to the zone of height above sea level 400~1500m when nourishing and growing end cycle red large variation strain YK01 kind strain growth 3~5 months;
E, reproductive growth period management: the red large variation strain YK01 kind strain seeding row spacing after the transplanting is 0.5 ~ 1.0m * 1.0 ~ 2.0m, mu is executed well-rotted farmyard manure 0~1000 Kg, the amount of mu applied nitrogen is purity nitrogen 0.0 ~ 7.0 Kg, nitrogen phosphorus potassium three essential fertilizer ratio is 1:0.5 ~ 2:2 ~ 4, the growing environment temperature is controlled at 5 ~ 35 ℃, oxygen content is controlled at 5 ~ 21%, and humidity is controlled at 50 ~ 90%, and illuminance is controlled at 1000 ~ 120000LX;
F, seed collection: spray 1 ‰~1% foliage fertilizer 0~5 time flowering stage in kind of a strain, behind seed maturity, gather.
2. the method in red large variation strain YK01 seminal propagation cycle of shortening according to claim 1 is characterized in that described transplanting is the zone of kind of strain being transplanted to height above sea level 1500~2500m.
3. the method in red large variation strain YK01 seminal propagation cycle of shortening according to claim 1 and 2 is characterized in that described transplanting is the zone of kind of strain being transplanted to height above sea level 1700~2500m.
4. the method in red large variation strain YK01 seminal propagation cycle of shortening according to claim 1 and 2 is characterized in that described transplanting is the zone of kind of strain being transplanted to height above sea level 2000~2500m.
5. the method in red large variation strain YK01 seminal propagation cycle of shortening according to claim 1 is characterized in that nitrogenous fertilizer in described C, the E step is one or more in farmyard manure, inorganic fertilizer, fertilizer or the bio-fertilizer.
6. the method in red large variation strain YK01 seminal propagation cycle of shortening according to claim 1 is characterized in that described transplanting is the zone of kind of strain being transplanted to height above sea level 400~1400m.
7. according to claim 1 or the method in red large variation strain YK01 seminal propagation cycle of 6 described shortenings, it is characterized in that described transplanting is the zone of kind of strain being transplanted to height above sea level 400~1200m.
8. according to claim 1 or the method in red large variation strain YK01 seminal propagation cycle of 6 described shortenings, it is characterized in that described transplanting is the zone of kind of strain being transplanted to height above sea level 400~1000m.
9. the method in red large variation strain YK01 seminal propagation cycle of shortening according to claim 1, it is characterized in that the well-rotted farmyard manure in the described E step is night soil, pig, ox, sheep, horsehit urine, bird fecaluria and barnyard manure, compost, biogas manure, smoked soil, a heatable brick bed are native, in the ash one or more.
10. the method in red large variation strain YK01 seminal propagation cycle of shortening according to claim 1, it is characterized in that in the described F step foliage fertilizer be high potassium foliage fertilizer.
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CN102124894A (en) * | 2010-12-27 | 2011-07-20 | 山东临沂烟草有限公司 | Technical method for cultivating cured tobaccos under mulching film |
CN102577818A (en) * | 2012-03-15 | 2012-07-18 | 四川农业大学 | Method for interplanting tobacco with soybean |
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CN102124894A (en) * | 2010-12-27 | 2011-07-20 | 山东临沂烟草有限公司 | Technical method for cultivating cured tobaccos under mulching film |
CN102577818A (en) * | 2012-03-15 | 2012-07-18 | 四川农业大学 | Method for interplanting tobacco with soybean |
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Title |
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