CN102952791B - 分批补加氮源培养真菌生产纤维素酶的方法 - Google Patents
分批补加氮源培养真菌生产纤维素酶的方法 Download PDFInfo
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- CN102952791B CN102952791B CN201210449827.1A CN201210449827A CN102952791B CN 102952791 B CN102952791 B CN 102952791B CN 201210449827 A CN201210449827 A CN 201210449827A CN 102952791 B CN102952791 B CN 102952791B
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- fermentation
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- nitrogen source
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 21
- 241000233866 Fungi Species 0.000 title claims abstract description 14
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 14
- 108010059892 Cellulase Proteins 0.000 title claims description 12
- 229940106157 cellulase Drugs 0.000 title claims description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 79
- 230000004151 fermentation Effects 0.000 claims abstract description 79
- 239000001913 cellulose Substances 0.000 claims abstract description 14
- 229920002678 cellulose Polymers 0.000 claims abstract description 14
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 125000001477 organic nitrogen group Chemical group 0.000 claims abstract description 11
- 239000002054 inoculum Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims description 51
- 102000004190 Enzymes Human genes 0.000 claims description 51
- 229940088598 enzyme Drugs 0.000 claims description 51
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 20
- 235000010980 cellulose Nutrition 0.000 claims description 11
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000000411 inducer Substances 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 8
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 6
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 6
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 6
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 241000228245 Aspergillus niger Species 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910002651 NO3 Inorganic materials 0.000 claims description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- 150000003863 ammonium salts Chemical class 0.000 claims description 2
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- 239000006052 feed supplement Substances 0.000 claims description 2
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 21
- 230000035764 nutrition Effects 0.000 abstract description 21
- 230000000694 effects Effects 0.000 abstract description 5
- 230000003204 osmotic effect Effects 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 abstract 3
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 13
- 241000499912 Trichoderma reesei Species 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 7
- 238000002203 pretreatment Methods 0.000 description 7
- 239000010902 straw Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 239000012092 media component Substances 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 3
- 101000765308 Aspergillus niger N-(5'-phosphoribosyl)anthranilate isomerase Proteins 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000006286 nutrient intake Nutrition 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
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- Enzymes And Modification Thereof (AREA)
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CN201210449827.1A CN102952791B (zh) | 2012-11-12 | 2012-11-12 | 分批补加氮源培养真菌生产纤维素酶的方法 |
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CN201210449827.1A CN102952791B (zh) | 2012-11-12 | 2012-11-12 | 分批补加氮源培养真菌生产纤维素酶的方法 |
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CN102952791A CN102952791A (zh) | 2013-03-06 |
CN102952791B true CN102952791B (zh) | 2014-12-03 |
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CN107354180B (zh) * | 2016-05-09 | 2021-09-07 | 中国石化扬子石油化工有限公司 | 一种产琥珀酸放线杆菌生产琥珀酸的调控方法 |
CN111500559A (zh) * | 2020-04-22 | 2020-08-07 | 湖南农业大学 | 一种纤维素酶发酵调控剂及应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101654669A (zh) * | 2009-09-02 | 2010-02-24 | 安徽丰原发酵技术工程研究有限公司 | 一种高产纤维素酶的方法 |
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Patent Citations (1)
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CN101654669A (zh) * | 2009-09-02 | 2010-02-24 | 安徽丰原发酵技术工程研究有限公司 | 一种高产纤维素酶的方法 |
Non-Patent Citations (1)
Title |
---|
蒲海燕.纤维素酶发酵工艺条件优化研究.《中国优秀博硕士学位论文全文数据库(硕士)工程科技I辑》.2005, * |
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Application publication date: 20130306 Assignee: Huaian hundred green green energy Co., Ltd. Assignor: Xiong Peng Contract record no.: 2015320000176 Denomination of invention: Method for replenishing nitrogen source culture fungus in batch to produce cellulase Granted publication date: 20141203 License type: Exclusive License Record date: 20150414 |
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