CN102940719A - Chinese herbal medicine feed additive for treating dairy cow mastitis - Google Patents
Chinese herbal medicine feed additive for treating dairy cow mastitis Download PDFInfo
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Abstract
The invention relates to a Chinese herbal medicine feed additive for treating dairy cow mastitis. The Chinese herbal medicine feed additive is prepared from 5 to 7 parts by weight of dandelion, 4 to 6 parts by weight of common andrographis herb, 2 to 4 parts by weight of heartleaf houttuynia herb, 2 to 4 parts by weight of weeping forsythia capsule, 2 to 4 parts by weight of honeysuckle, 2 to 4 parts by weight of wild chrysanthemum, 1 to 2 parts by weight of coptis, 2 to 3 parts by weight of baical skullcap root and 1 to 2 parts by weight of liquorice root. The Chinese herbal medicine feed additive can be used for treating dairy cow mastitis and has the characteristics of short treatment course, low cost, high cure rate, good safety, no toxic and side effect, and taking convenience.
Description
Technical field
The present invention relates to the Chinese herbal and crude drugs preparations that a kind of milch cow is used, be specifically related to a kind of Chinese herbal feed additive for the treatment of mammitis of cow.
Background technology
Mastitis is the common principal disease of harm milk cow health.The pathogenic microorganism of mammitis of cow is kind more than 80 nearly, common are 23 kinds, and wherein antibacterial is 14 kinds, 2 kinds of Mycoplasmas, and 7 kinds in fungus and virus, but 80%～90%, caused by staphylococcus and streptococcus.The loss that mastitis causes comprises that mainly milk production reduces, and milk-quality reduces, and milk is discarded, increases the cost of reserve milch cow, extra labor expenses, medicine spending and veterinary service expense etc.China's clinic mastitis sickness rate is 21%～23%, and the subclinical mastitis sickness rate is about 50%.According to Chinese veterinarian's theory, except the mastitis that wound causes, all be that the breast channels is not smooth, galactostasis heating corruption forms, therefore answer dispersing liver-energy, Disperse hepatic depression when treating, clearing away stomach-heat, clear toxic swelling, lactogenesis network.At present, the medicine for the treatment of mammitis of cow is a lot, and adopting more is the Antibiotics Western medicine, although have certain curative effect, shortcoming is that Drug resistance and side effect are more serious, and at meat with to have chemicals in suckling residual, brings potential threat to human health; That Chinese herbal medicine has is natural, have no side effect,, the characteristics such as be difficult for develop immunity to drugs residual without chemicals, be used for mammitis of cow diseases prevention, cure the disease, be with a wide range of applications.But the present commercially available Chinese herbal medicine that is used for the treatment of mammitis of cow, result of use is poor, and cure rate is low, and the course for the treatment of is long and cost is high.
Summary of the invention
The object of the invention is to for the problems referred to above, a kind of Chinese herbal feed additive that can effectively treat mammitis of cow is provided.
Technical scheme of the present invention is: a kind of Chinese herbal feed additive for the treatment of mammitis of cow, it is the medicament of being made by the raw material of following weight proportioning: Herba Taraxaci 5-7 part, Herba Andrographis 4-6 part, Herba Houttuyniae 2-4 part, Fructus Forsythiae 2-4 part, Flos Lonicerae 2-4 part, Flos Chrysanthemi Indici 2-4 part, Rhizoma Coptidis 1-2 part, Radix Scutellariae 2-3 part, Radix Glycyrrhizae 1-2 part.
The preferred feedstock weight proportion of making the Chinese herbal feed additive that the present invention treats mammitis of cow is: 7 parts of Herba Taraxacis, 5 parts of Herba Andrographis, 4 parts of Herba Houttuyniae, 4 parts of Fructus Forsythiaes, 2 parts of Flos Loniceraes, 4 parts of Flos Chrysanthemi Indicis, 1 part of Rhizoma Coptidis, 2 parts of Radix Scutellariaes, 1 part in Radix Glycyrrhizae.
The each component pharmacological effect is in the prescription of the present invention:
Herba Taraxaci effect: contain taraxasterol, choline, inulin, pectin in the Herba Taraxaci plant; Taraxol, stigmasterol, β-Amyrin, cupreol, taraxerol, taraxacin, taraxacin and vitamin A, B, C etc.The property sweet, little hardship, cold.Dandelion Injectable Solution has stronger bactericidal action to staphylococcus aureus resistance bacterial strain, Hemolytic streptococcus in vitro, and Diplococcus pneumoniae, meningococcus, diphtheria corynebacterium, bacillus pyocyaneus, Bacillus proteus, dysentery bacterium, Bacillus typhi etc. are also had certain bactericidal action; Extracting solution 1:400 can suppress tulase in vitro, and alcohol extract 31mg/kg can kill leptospira.The Herba Taraxaci decoct can significantly improve human peripheral lymphocyte blast transformation rate external, and the Taraxacum Polysaccharides lumbar injection can significantly strengthen the cytotoxicity of mouse antibodies dependency macrophage.Herba Taraxaci tool heat-clearing and toxic substances removing, the effect of dispersing swelling and dissipating binds.Cure mainly upper respiratory tract infection, eye conjunctivitis, mumps, acute mastitis swells and ache, gastritis, dysentery, hepatitis, gallbladder is assisted inflammation, carbuncle furuncle furuncle, and pharyngitis is controlled acute mastitis, lymphadenitis, scrofula, the furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, urinary tract infection.
The Herba Andrographis effect: Herba Andrographis has another name called Chun Lianqiuliu, Herba Andrographitis, Radix Gentianae, golden Rhizoma et radix valerianae, Tremella aurantialba Bandoni et Zang hook, India's grass, Herba vallisneriae Spiralis etc.Leaf contains Diterpenes such as andrographolide 0.6%, dexyandrographolide 0.15%, neoandrographolide 0.05%, dexyandrographolide-19-β-D-Glucose glycoside etc., flavonoid, Polyphenols such as caffeic acid, chlorogenic acid and two caffeoyl silicon thujic acid mixture.Root contains flavonoid such as andrographin etc., and aerial parts contains Diterpenes such as andrographolide etc.Andrographolide, neoandrographolide all have the effect that suppresses and delay Diplococcus pneumoniae and the caused fervescence of hemolytic group B streptococcus, increase with dimethylbenzene, acetic acid induced mice skin or abdominal cavity capillary permeability, gavage deoxyandrographolide or dehydrorographolide all can reduce oozing out of capillary wall.The Herba Andrographis water decoction can improve the ability that peripheral blood leucocyte is engulfed staphylococcus aureus external.Herba Andrographis tool heat-clearing and toxic substances removing, the effect of removing heat from blood detumescence.Be used for the multi-infection disease, comprise trauma infection contamination, furuncle, carbuncle, erysipelas, upper respiratory tract infection, acute and chronic tonsillitis, acute and chronic pharyngitis, acute/chronic bronchitis, acute bacillary dysentery, acute gastroenteritis, urinary tract infection, endometritis, pelvic inflammatory disease etc.
The Herba Houttuyniae effect: Herba Houttuyniae another name Herba Houttuyniae, smelly dish, Herba Houttuyniae, smelly grass, Herba laggera, Zhu's skin encircle.Cold nature, bitter in the mouth.Return lung meridian, bladder, large intestine channel.The Herba Houttuyniae herb contains volatile oil, and wherein active ingredient is that decanoylacetaldehyde is houttuynine sodium bisulfite, lauryl aldehyde, methyln nonyl ketone, Flos Caryophylli alkene, linalool, borneol acetate, australene, camphene, myrcene and (R)-4-isopropenyl-1-methyl-1-cyclohexene, methyl n nonyl ketone, capraldehyde, capric acid.All contain Quercetin, quercitrin, Isoquercitrin, reynoutrin, hyperin, afzerin, globulariacitrin in flower, leaf, the fruit.Still contain chlorogenic acid, Palmic acid, oleic acid, oleic acid; Potassium chloride, sulphuric acid, and cupreol etc.Herba Houttuyniae has obvious inhibitory action to Hemolytic streptococcus, staphylococcus aureus, hemophilus influenza, micrococcus catarrhalis, streptococcus pneumoniae.Escherichia coli, dysentery bacterium, Bacillus typhi also there is effect.Houttuynine sodium bisulfite III influenza virus infected has the preventive protection effect, and the derivant of Sodium Houttuyfonate also has stronger antivirus action.The mice that Herba Houttuyniae extracts from crude drugs 4g/mL infected by influenza infects has obvious preventive effect.Experiment in vitro proof Herba Houttuyniae water extraction liquid can suppress 1 type and herpes simplex types 2 viral infection.Herba Houttuyniae tool heat-clearing and toxic substances removing, removing pus and relieving carbuncle, the effect of inducing diuresis for treating stranguria syndrome; Cure mainly the lung abscess vomiting pus, the expectorant dyspnea of heat type is coughed, throat, hematodiarrhoea, carbuncle sore tumefacting virus.
The Fructus Forsythiae effect: Fructus Forsythiae has another name called that Hemerocallis citrina Baroni bar, Fructus Forsythiae, green grass or young crops stick up, fall to sticking up, Huang Qidan etc., fruit medicine.The medicinal part of Fructus Forsythiae mainly is fruit.Contain forsythol, forsythin, phillygenin, oleanolic acid, betulic acid, ursolic acid, pinoresinol, horse tongue resinol glycoside, still contain multiple hydro carbons, aldoketones, alcohol ester ethers volatile ingredient such as australene, camphene, nopinene etc., Camphora, geranial, Borneolum Syntheticum, α-terpinol, safrole, linalool, Terpinen-4-ol, nor-lapachol and coumarin, rutin etc.Fructus Forsythiae all has inhibitory action to multiple Grain-positive and negative bacteria.Its concentrated decotion suppresses Bacillus typhi, Salmonella paratyphi, escherichia coli, dysentery bacterium, diphtheria corynebacterium and vibrio cholera, staphylococcus, streptococcus etc. external.Experiment in vitro shows: the alcohol extract of Fructus Forsythiae has anti-leptospira effect, Embryo Gallus domesticus in vitro tests proof Fructus Forsythiae has inhibitory action to Asia influenza A virus, rhinovirus-17 type, Semen Fructus Forsythiae volatile oil also has obvious antibacterial action external to staphylococcus aureus, also has obviously to suppress the staphylococcus aureus plasma-coagulase to the consolidation of blood plasma.The staphylococcus aureus that infects in the rabbit blood is obviously reduced, and Fructus Forsythiae water logging agent 1:5 has some inhibitory action to star-shaped nocardia in vitro.Fructus Forsythiae decoct or compound phillyrin injection have cooling effect to artificial fever animal and intact animal's body temperature.Fructus Forsythiae tool heat-clearing and toxic substances removing, the effect of dispersing swelling and dissipating binds.Be used for carbuncle, scrofula, acute mastitis, erysipelas, anemopyretic cold, epidemic febrile disease is from the beginning of, warm joining the army, high hot excessive thirst, coma is sent out speckle, the pyretic stranguria urine retention.
The Flos Lonicerae effect: Flos Lonicerae is Caprifoliaceae woodbine Radix Ophiopogonis and congener dry flower or the flower just opened.Flos Lonicerae contains chlorogenic acid, isochlorogenic acid, Ginnol, cupreol, stigmasterol, cupreol-D-Glucose glycoside, stigmasterol-D-Glucose glycoside; Also contain volatile oil, its composition has linalool, left-handed-along trimethyl-2-vinyl-5-hydroxyl-Pentamethylene oxide., ethyl palmitate, 1,1-di-thiacyclohexane, methyl linoleate, 3-methyl-2-(2-pentenyl)-the 2-cyclopentene-1-one, ethyl linolenate, suitable-3-is alkene-1-alcohol, alpha-terpineol, tens of kinds of eugenol and carvacrols etc.The property sweet cold fragrance, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil.Experiment in vitro shows, flower and rattan all have certain inhibitory action to various pathogens such as staphylococcus aureus, Hemolytic streptococcus, escherichia coli, dysentery bacterium, vibrio cholera, Bacillus typhi, Salmonella paratyphi etc., and be also effective to streptococcus pneumoniae, meningococcus, bacillus pyocyaneus, tubercule bacillus.The water logging agent is stronger than decoct effect, and the leaf decoct is stronger than colored decoct effect.Flos Lonicerae tool dispelling wind-heat, the effect of removing summer-heat blood poison is used for various febrile diseases, such as cards such as fever of the body, dermexanthesis, a speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain.
Flos Chrysanthemi Indici effect: Flos Chrysanthemi Indici another name FLOS CHRYSANTHEMI INDICI flower, the bitter heart of a lotus seed, mountain Flos Chrysanthemi, Flos Chrysanthemi.Chemical analysis contains volatile oil, contains alantol, chrysanthanone, australene, Camphora, Borneolum Syntheticum, lauro lene etc. in the oil, still contains yejuhua lactone, artoglasin A, acaciin, linarin, chrysanthemum glycoside, luteolin.Be slightly cold; Hot, bitter; Return lung, Liver Channel.To staphylococcus aureus, diphtheria corynebacterium, streptococcus, bacillus pyocyaneus, dysentery bacterium, influenza virus, inhibitory action is arranged all.The Flos Chrysanthemi Indici water decoction is 2.5mg/mL to the minimum inhibitory concentration of strong malicious M. tuberculosis mycobacteria, and its antibacterial principle is that bacterial cell wall is changed, and destroys or disappearance, and its ultrastructure is obviously destroyed.Flos Chrysanthemi Indici or Flos Chrysanthemi Indici branch and leaf 1:5 decoct have the effect that suppresses dysentery bacterium in vitro, and branch and leaf still have the effect that suppresses Bacillus typhi.The Flos Chrysanthemi Indici decoct also has inhibitory action to other various pathogens, and MIC also has in various degree inhibitory action to the various skin fungus during high concentration between 1:10-1:80.Flos Chrysanthemi Indici tool heat-clearing and toxic substances removing, the effect of detumescence.Be used for upper sense, influenza, acute suppurative inflammation, laryngopharynx swelling and pain, scrofula disease, conjunctival congestion and swelling pain etc.
Rhizoma Coptidis effect: Rhizoma Coptidis another name Wang Lian, Zhi Lian.Dry rhizome for cohosh Rhizoma Coptidis, Coptis deltoidea C.Y.Cheng et Hsiao and Coptis Teeta Wall.(root) hardship, cold, nontoxic.GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel.Rhizome contains multiple alkaloid, mainly is berberine, claims again berberine, is coptisine, 13-methyl-.psi.-coptisine., palmatine, jateorhizine, Columbamine secondly.Still contain obacunone, obakulactone, magnoflorine, ferulic acid etc.Leaf contains berberine.In addition, also contain various trace elements in the Rhizoma Coptidis.Have the effects such as anti-Staphylococcus aureus, Hemolytic streptococcus, streptococcus pneumoniae, meningococcus, dysentery bacterium, anthrax bacillus, inhibition influenza virus, hepatitis B virus.The pharmacologically active such as antitumor and arrhythmia also has blood sugar lowering, antitumor, protection gastric mucosa isoreactivity.The antagonism bacteriotoxin, reduction staphylococcus aureus coagulase, hemolysin are tired, and reduce colibacillary virulence.Rhizoma Coptidis tool heat clearing and damp drying, the effect of eliminating fire and detoxication.Be used for damp and hot feeling of fullness, the vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood is told nosebleed, conjunctival congestion, the carbuncle furuncle is quenched one's thirst in toothache; External treatment eczema, eczema, auditory meatus is suppurated.
Radix Scutellariae effect: Radix Scutellariae another name Radix Scutellariae, scutellaria tea, tsuchikane tea root etc.Hardship, cold.Return lung, gallbladder, spleen, large intestine, small intestine meridian.Scutellariae,radix contains flavone compound: baicalin, and neobaicalein, baicalin, wogonin, wogonoside, oroxylin A, 7-methoxyl group baicalin, the dihydro oroxylin, chrysin also contains the active ingredients such as cupreol, campesterol and stigmasterol in addition.Dysentery bacterium, Bacillus typhi, Salmonella paratyphi, vibrio cholera, escherichia coli, Bacillus proteus, bacillus pyocyaneus, staphylococcus, hemolytic vessel used to hold grain at the imperial sacrifice coccus, Diplococcus pneumoniae, diphtheria corynebacterium etc. there is inhibitory action.Radix Scutellariae tool heat clearing and damp drying, removing heat from blood is antiabortive, the effect of detoxifcation.Cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.
The Radix Glycyrrhizae effect: Radix Glycyrrhizae mainly contains the compositions such as glycyrrhizic acid, glycyrrhizin, 24-hydroxyl enoxolone, licoflavone, glycyrrhizin, isoliquiritigenin, Angelica Polysaccharide, has antiulcer, promotes pancreatic secretion, protects the liver, the effects such as phagocytic function of blood fat reducing, adjusting macrophage.Glycyrrhizic acid is natural antidote, can be used for body defying age, enhancing immunity, keeps water-electrolyte metabolism balance etc.; Angelica Polysaccharide has anti-adenovirus type III, herpes simplex virus I-form is active.Has invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, the effect of coordinating the actions of various ingredients in a prescription.Be used for weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, the anxious pain of extremity contraction, carbuncle sore tumefacting virus, cushion toxicity, strong.
Prescription foundation of the present invention: mastitis is acute mastitis, is that expectorant, wet, gas, blood stagnation knot are loose, changes and is scorching.Or because of excessiveness of stomach-heat, stagnation of QI due to depression of the liver, newborn network lose smooth so that breast coagulation of QI-blood, stasis and give birth to carbuncle; Or be not good at because of feeding and management, the ground that lying on bed for a long time is damp and hot steams on the damp and hot poison, the infringement breast; And latent mammitis is many because of the body insufficiency of vital energy and blood, again due to the being invaded by exogenous pathogen.Therefore, prescription should be take blood circulation promoting and blood stasis dispelling, heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, the stimulating milk secretion of stimulating the menstrual flow as principle.The Herba Taraxaci hardship is rushed down to fall, and is sweet with detoxifcation, the heat clearing away of cold energy, hold concurrently with lactogenesis, be the key medicine of controlling acute mastitis, contain the multiple health-nutrition compositions such as taraxol, taraxacin, choline, organic acid, inulin in the Herba Taraxaci plant, have the effect of heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, inducing diuresis for treating stranguria syndrome; Heat-clearing and toxic substances removing, the mass dissipating and swelling eliminatings such as Fructus Forsythiae, Herba Taraxaci, Flos Lonicerae.Wherein, it is cold clear that the Fructus Forsythiae hardship is rushed down, clearing away heart-fire and removing toxicity for eliminating carbuncles rushes down gallbladder-heat and the resolving depression eliminating stagnation, and its Main Ingredients and Appearance is forsythol and volatile component.Herba Andrographis contains andrographolide, flavonoid, Polyphenols, has heat-clearing and toxic substances removing, antiinflammatory, reducing swelling and alleviating pain effect; Herba Houttuyniae contains houttuynine sodium bisulfite, lauryl aldehyde, chlorogenic acid etc., has the effect of stronger anti-inflammation, heat-clearing and toxic substances removing, removing pus and relieving carbuncle; Fructus Forsythiae contains forsythol, sterol compound, Saponin and Flavonoids, horse tongue resinol glycoside etc., has stronger antibacterial action; Flos Chrysanthemi Indici, Rhizoma Coptidis, Radix Scutellariae all have more widely antibacterial and anti-inflammation functions.With the sweet temperature QI invigorating of Radix Glycyrrhizae and middle spleen invigorating, current twelve channels and collaterals, coordinating the actions of various ingredients in a prescription.In the side all medicine phases and, play altogether blood circulation promoting and blood stasis dispelling, heat-clearing and toxic substances removing, anti-inflammation, dispersing swelling and dissipating binds, the stimulating milk secretion of stimulating the menstrual flow, improve the effect of immunity of organisms and resistance against diseases.Be applicable to the mammitis of cow that a variety of causes causes.Using, make powder for convenient.
The present invention treats the Chinese herbal feed additive of mammitis of cow, on the basis of having carried out a large amount of practical applications, according to the traditional medicine ancient prescription, form in conjunction with sick of modern popular, nosetiology and immunologic theoretical screening, be used for the treatment of mammitis of cow, short treating period, cost is low, cure rate is high, safely, have no side effect, taking convenience.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment:
Take by weighing respectively raw material (decoction pieces): Herba Taraxaci 350 g, Herba Andrographis 250 g, Herba Houttuyniae 200 g, Fructus Forsythiae 200 g, Flos Lonicerae 100 g, Flos Chrysanthemi Indici 200 g, Rhizoma Coptidis 50 g, Radix Scutellariae 100 g, Radix Glycyrrhizae 50 g.Above-mentioned raw materials is ground into respectively the Chinese herbal medicine coarse powder, mixes, packing namely gets the Chinese herbal feed additive that the present invention treats mammitis of cow.
Some test situation that the Chinese herbal feed additive that utilizes the present invention to treat mammitis of cow carries out are as follows:
1, feed additive of the present invention is to the drug sensitive test of bovine subclinical mastitis the main pathogenic fungi
Choose clinical trouble latent mammitis milch cow case, through separating, identifying, select pathogenic stronger 2 kinds of pathogen such as staphylococcus aureus and streptococcus agalactiae.Take by weighing a certain amount of feed additive of the present invention, add 10 times of amount distilled water heating and refluxing extraction 2 times, each 0.5h merges secondary filtrate, and be evaporated to 1mL liquid and be equivalent to crude drug in whole 1.0g (1g/mL), 100 ℃ of boiling sterilizations, 4 ℃ save backup.Staphylococcus aureus, the streptococcus agalactiae of clinical separation are inoculated in first flat board, behind 37 ℃ of cultivation 18 h, select colonies typical and be inoculated in the 5mL meat soup, behind 37 ℃ of cultivation 18h, 0.5 Maxwell unit's standard opacity tube is diluted to 10 with meat soup with bacterium liquid than turbid
5CFU/mL, for subsequent use.
(1) medicament sensitivity test adopts the plate punch method, adds the crude drug extracting solution of 1g/mL feed additive of the present invention in the hole, and overfolw hole is not advisable outward to fill it up with.Other gets penicillin 10IU/ sheet, ampicillin 5 μ g/mL, three kinds of antibiotic drug sensitive tests of ciprofloxacin 1 μ g/mL and compares, and places 37 ℃ of constant incubators to cultivate 24h, measures antibacterial circle diameter, the drug sensitive test triplicate of every kind of medicine.Criterion: according to the standard of " pharmacology of Chinese materia medica " and " U.S. clinical laboratory Standardization Research the relevant drug sensitive test standardization of version in 2005 ", namely bacteriostatic diameter 〉=20mm is for extremely quick, 20mm〉bacteriostatic diameter 〉=15mm is Gao Min; 15mm〉bacteriostatic diameter 〉=10mm is quick in being, and 10mm is following to be hypoallergenic.
(2) the mensuration micro-dilution method of minimal inhibitory concentration (MIC): in each hole of the every row of 96 orifice plates 1-10 of sterilization, the crude drug extracting solution that adds successively the feed additive of the present invention of using the meat soup doubling dilution with micropipettor, every hole 200 μ L, totally 10 different concentration, then add dilution bacterium liquid 10 μ L in every hole, the 11st, 12 holes are respectively negative control group (not adding pathogenetic bacteria) and positive controls (not adding medicine).Place the 1min that vibrates on the agitator, make in the hole behind the abundant mixing of solution, microwell plate is added a cover and with being placed in the square enamel tray that is covered with wet gauze after the gummed paper sealing, is hatched 18h in 37 ℃ of incubators, observes the contained lowest concentration of drug in asepsis growth hole with eye and is minimal inhibitory concentration MIC.Record other three kinds of antibiotic such as penicillin, ampicillin, ciprofloxacin minimal inhibitory concentration MIC with method.
(3) mensuration of minimal bactericidal concentration (MBC) is drawn respectively each the hole culture that has no bacterial growth more than the 0.1mL said medicine minimal inhibitory concentration, move on the plate agar culture medium of pastille not, push gently culture open with aseptic " L " type Glass rod, put overnight incubation in 37 ℃ of incubators, observation has asepsis growth, and counting is less than the minimal bactericidal concentration MBC that 5 bacterium colony persons are this medicine.Record other three kinds of antibiotic such as penicillin, ampicillin, ciprofloxacin minimal bactericidal concentration MBC with method.
The crude drug extracting solution medicament sensitivity test of feed additive of the present invention the results are shown in Table 1, and the crude drug extracting solution of feed additive of the present invention sees Table 2 to MIC, the MBC result of the test of staphylococcus aureus and streptococcus agalactiae.
Table 1 feed additive of the present invention is to the drug sensitive tests of two kinds of bacterium mm as a result
| Medicine | Staphylococcus aureus | Streptococcus agalactiae |
| Penicillin | 21.75±2.02 | 19.87±1.86 |
| Ampicillin | 20.73±1.21 | 22.44±1.88 |
| Ciprofloxacin | 20.22±1.55 | 18.79±1.44 |
| The crude drug extracting solution of feed additive of the present invention | 23.85±1.79 | 24.30±1.72 |
Table 1 demonstration, to staphylococcus aureus, the crude drug extracting solution of feed additive of the present invention is for extremely quick, and bacteriostatic diameter reaches 23.85mm, and penicillin, ampicillin and ciprofloxacin are Gao Min; To streptococcus agalactiae, the crude drug extracting solution of feed additive of the present invention is for extremely quick, and bacteriostatic diameter reaches 24.3mm, and other medicines such as penicillin, ampicillin and ciprofloxacin are Gao Min.
Table 2 feed additive of the present invention is to MIC, the MBC result of the test g/mL of two kinds of bacterium
Table 2 shows, the crude drug extracting solution of feed additive of the present invention is stronger to inhibition and the killing action of staphylococcus aureus and streptococcus agalactiae, staphylococcus aureus is minimum to be suppressed (MIC) and kills (MBC) concentration can reach 0.008g/mL, 0.016g/mL respectively, and streptococcus agalactiae is minimum to be suppressed (MIC) and kill (MBC) concentration 0.004g/mL.Other medicines such as penicillin, ampicillin and ciprofloxacin take second place.
Drug sensitivity test shows, staphylococcus aureus and streptococcus agalactiae are the most responsive to the crude drug extracting solution of feed additive of the present invention; Ampicillin takes second place; The crude drug extracting solution of feed additive of the present invention is stronger to inhibition and the killing action of staphylococcus aureus and streptococcus agalactiae, and ampicillin takes second place.The crude drug extracting solution of feed additive of the present invention is good than antibiotic such as penicillins to the inhibition of 2 kinds of pathogen of bovine subclinical mastitis, should be as first-selection in the practice of control bovine subclinical mastitis.
2, feed additive of the present invention is on the impact of milk cow production performance
32 He Sitan lactating cows are divided into 4 groups at random by test requirements document, and 8 every group, to feed respectively and in concentrate, add the daily ration of 0.2%, 0.4%, 0.6% feed additive of the present invention, test schedules to last 60d.The result shows: the examination cattle of adding 0.2%, 0.4%, 0.6% feed additive in the feedstuff is improved milk yield than matched group and reaches respectively 4.02%, 12.5%, 14.0% (P〉0.05), milk nutritive index (butterfat, lactoprotein, lactose, total milk solid) is not determined impact, but can significantly reduce somatic number.Feed additive of the present invention sees Table 3 to the EXPERIMENTAL DESIGN scheme that affects of milk cow production performance, and each test group milk production of cow statistical result sees Table 4, and each test group milk quality refers to that measurement result sees Table 5, and each organizes concentrated feed and the roughage consumption sees Table 6.
Table 3 EXPERIMENTAL DESIGN scheme
| Group | Design |
| Test 1 group | Basal diet+0.2% feed additive of the present invention |
| Test 2 groups | Basal diet+0.4% feed additive of the present invention |
| Test 3 groups | Basal diet+0.6% feed additive of the present invention |
| Matched group | Basal diet |
Each test group milk production of cow statistical table unit of table 4: kg, %
| Group | Individual average daily milk yield in experimental period | Increase milk yield than matched group | Increase percentage ratio |
| Test 1 group | 20.43±1.08 a | 0.79 | 4.02 |
| Test 2 groups | 22.10±0.89 b | 2.64 | 12.5 |
| Test 3 groups | 22.39±1.02 b | 2.75 | 14.0 |
| Matched group | 19.64±0.95 a | ? | ? |
Annotate: same column lower case identical table differential is different not remarkable, the different expression of same column lower case significant difference, and the different expression of capitalization difference is extremely remarkable.Lower same.
Table 4 shows, test 1,2,3 group and matched group in experimental period daily individual milk yield be respectively 20.43 ± 1.08kg, 22.10 ± 0.89kg, 22.39 ± 1.02kg and 19.64 ± 0.95kg.In trial stretch, with the increase of Chinese herbal medicine powder addition of the present invention in the daily ration, milk crop is increase trend; Testing 2,3 groups of average daily milk yield is significantly higher than matched group and tests 1 group (P＜0.05), test 1 group and matched group difference not significantly (P〉0.05), test 1,2 and 3 group of average daily milk yield respectively than matched group raising 4.02%, 12.5%, 14.0%.
Table 5 feed additive of the present invention is on the unit that affects of the every quality index of milk: %
| Group | Butter oil | Lactoprotein | Total solid |
| Test 1 group | 3.16±0.32 a | 2.93±0.09 a | 8.19±0.16 |
| Test 2 groups | 3.34±0.21 a | 2.95±0.02 a | 8.18±0.25 |
| Test 3 groups | 3.49±0.12 a | 2.94±0.05 a | 8.21±0.33 |
| Matched group | 3.12±0.25 a | 2.92±0.03 a | 8.14±0.12 |
| GB19645-2005 | ≥3.1 | ≥2.9 | ≥8.1 |
Table 5 shows, the butter oil that each test group early stage, mid-term and later stage three sampling are measured, lactoprotein, total solid etc., every quality index all meets pasteurize, sterilized milk state health standards (GB 19645-2005), increase with Chinese herbal medicine powder addition of the present invention, the milk butterfat percnetage has increase trend, and (difference is not remarkable, P〉0.05), content of milk protein and total solid are basically identical between each group.The full phase is respectively organized the concentrated feed consumption and reduces with the increase of feed additive addition of the present invention, matched group is higher than 1,2 group (P＜0.05) of test significantly, the utmost point is higher than 3 groups (P＜0.01) of test significantly, test 1,2,3 group of every consumption 1 kg concentrate supplement, the average daily individual milk yield of milch cow improves 0.24kg, 0.31kg and 0.60kg than matched group.The full phase is respectively organized roughage and consumes and also have the trend that the increase with feed additive addition of the present invention reduces, and test 2,3 groups of roughages and consumes and significantly be lower than matched group (P＜0.05), and the utmost point is markedly inferior to tests 1 group (P＜0.01).
Table 6 is respectively organized feed consumption statistical table unit: kg
Table 6 shows, test 1,2 and 3 group of average daily individual milk yield and improve 0.79kg, 2.64kg and 2.75kg than matched group respectively, daily increase the output value of 2.37 yuan, 7.92 yuan and 8.25 yuan than matched group, annual (press 305d calculates) is respectively than the income of 722.80 yuan, 2415.60 yuan of matched group increases and 2516.30 yuan; Test the equal day feed cost of 1,2,3 group of head and drop into-0.25 yuan ,-0.47 yuan and-0.67 yuan than matched group more, each is organized every sun and adds feed additive cost of the present invention and be respectively 0.31 yuan, 0.62 yuan and 0.93 yuan, and annual (press 305d calculates) is respectively than the feed cost usefulness of matched group saving-18.30 yuan ,-45.75 yuan and-79.30 yuan; Two totals are tested 1,2,3 group than 704.50 yuan, 2369.85 yuan and 2437.00 yuan of the equal year newly-increased incomes of matched group head.
1,2,3 test group of the present invention is added respectively 0.2%, 0.4%, 0.6% feed additive of the present invention, and the average daily milk yield of milch cow improves 4.02%, 12.5%, 14.0% than matched group respectively; Each is organized the quality index such as butter oil, lactoprotein, non-fat solid content and meets pasteurize, sterilized milk state health standards (GB 19645-2005); And with the increase of feed additive addition of the present invention, the milk butterfat percnetage has increase trend; Lactoprotein, non-fat solid content are basically identical.The full phase is respectively organized concentrated feed consumption and reduces with the increase of feed additive addition of the present invention, tests 1,2,3 group of every consumption 1kg concentrate supplement, and the average daily individual milk yield of milch cow increases 0.19kg, 0.38kg and 0.51kg than matched group.Experimental result shows, the Chinese herbal feed additive that the present invention treats mammitis of cow has specific disease-prevention health effect, to the certain effect that has of control mammitis of cow.
Claims (2)
1. Chinese herbal feed additive for the treatment of mammitis of cow, it is characterized in that it is the medicament of being made by the raw material of following weight proportioning: Herba Taraxaci 5-7 part, Herba Andrographis 4-6 part, Herba Houttuyniae 2-4 part, Fructus Forsythiae 2-4 part, Flos Lonicerae 2-4 part, Flos Chrysanthemi Indici 2-4 part, Rhizoma Coptidis 1-2 part, Radix Scutellariae 2-3 part, Radix Glycyrrhizae 1-2 part.
2. the Chinese herbal feed additive for the treatment of mammitis of cow according to claim 1, it is characterized in that it is the medicament of being made by the raw material of following weight proportioning: 7 parts of Herba Taraxacis, 5 parts of Herba Andrographis, 4 parts of Herba Houttuyniae, 4 parts of Fructus Forsythiaes, 2 parts of Flos Loniceraes, 4 parts of Flos Chrysanthemi Indicis, 1 part of Rhizoma Coptidis, 2 parts of Radix Scutellariaes, 1 part in Radix Glycyrrhizae.
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| CN103340312A (en) * | 2013-07-11 | 2013-10-09 | 曾勇 | Feed additive for preventing dairy cow mastitis |
| CN103652501A (en) * | 2013-12-25 | 2014-03-26 | 山东新希望六和集团有限公司 | Feed for treating cow mastitis and preparation method thereof |
| CN104161176A (en) * | 2013-07-15 | 2014-11-26 | 河南联合英伟饲料有限公司 | Traditional Chinese medicine feed additive for effectively preventing dairy cow mastitis |
| CN104187053A (en) * | 2014-07-24 | 2014-12-10 | 宿州市草源牧业股份有限公司 | Composite feed for preventing dairy cow mastitis |
| CN104288356A (en) * | 2014-09-17 | 2015-01-21 | 河北科星药业有限公司 | Traditional Chinese medicine perfusate for treating cow mastitis and preparation method thereof |
| CN104489279A (en) * | 2015-01-15 | 2015-04-08 | 河北科技师范学院 | Chinese traditional herb feed additive for treating cow mastitis |
| CN105918660A (en) * | 2016-04-28 | 2016-09-07 | 青岛海之星生物科技有限公司 | Cow feed additive and preparation method thereof |
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| CN107823375A (en) * | 2017-11-27 | 2018-03-23 | 青岛农业大学 | A kind of Chinese medicinal tablet for treating mastitis for milk cows and preparation method thereof |
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| CN114931602A (en) * | 2022-06-14 | 2022-08-23 | 河北科技师范学院 | Chinese herbal medicine formula for inhibiting dairy cow escherichia coli and preparation method thereof |
-
2012
- 2012-09-05 CN CN201210325138XA patent/CN102940719A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 代敏等: "奶牛***炎病原菌对中药的敏感性试验", 《江苏农业科学》 * |
| 崔一喆: "中药注射液的筛选及其对奶牛***炎的防治作用", 《中国优秀博硕士学位论文全文数据库 (硕士) 农业科技辑》 * |
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| CN103340312B (en) * | 2013-07-11 | 2014-04-02 | 曾勇 | Feed additive for preventing dairy cow mastitis |
| CN104161176A (en) * | 2013-07-15 | 2014-11-26 | 河南联合英伟饲料有限公司 | Traditional Chinese medicine feed additive for effectively preventing dairy cow mastitis |
| CN103652501A (en) * | 2013-12-25 | 2014-03-26 | 山东新希望六和集团有限公司 | Feed for treating cow mastitis and preparation method thereof |
| CN104187053A (en) * | 2014-07-24 | 2014-12-10 | 宿州市草源牧业股份有限公司 | Composite feed for preventing dairy cow mastitis |
| CN104288356A (en) * | 2014-09-17 | 2015-01-21 | 河北科星药业有限公司 | Traditional Chinese medicine perfusate for treating cow mastitis and preparation method thereof |
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| CN105918631A (en) * | 2016-04-28 | 2016-09-07 | 青岛海之星生物科技有限公司 | Feed additive for preventing cow subclinical mastitis and preparation method thereof |
| CN105918660A (en) * | 2016-04-28 | 2016-09-07 | 青岛海之星生物科技有限公司 | Cow feed additive and preparation method thereof |
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| CN107854555A (en) * | 2017-11-17 | 2018-03-30 | 锦州景良动物药业有限公司 | It is a kind of to be used to treat traditional Chinese medicine perfusion agent of mastitis for milk cows and preparation method thereof |
| CN107823375A (en) * | 2017-11-27 | 2018-03-23 | 青岛农业大学 | A kind of Chinese medicinal tablet for treating mastitis for milk cows and preparation method thereof |
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| CN110037177A (en) * | 2018-01-17 | 2019-07-23 | 内蒙古自治区农牧业科学院 | A kind of cow feed additive and its preparation and application |
| CN110037177B (en) * | 2018-01-17 | 2022-08-23 | 内蒙古自治区农牧业科学院 | Milk cow feed additive and preparation and use methods thereof |
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