Summary of the invention
For the deficiency of current oral ulcer clinical treatment medicine, first object of the present invention is to provide a kind of Chinese medicine composition for the treatment of oral ulcer, and this Chinese medicine composition has good therapeutic effect in treatment acute oral ulcer, chronic stomatocace and anaphylaxis oral ulcer.
The present invention treats the Chinese medicine composition of oral ulcer, obtained by the raw material of following weight portion: Radix Scutellariae 12-16 part, Flos Lonicerae 5-8 part, Pericarpium Granati 8-12 part, Calyx Seu Fructus Physalis 8-12 part, Radix Asparagi 8-12 part, Herba Taraxaci 18-22 part, Periostracum Cicadae 18-22 part, Folium Nelumbinis 8-12 part, Radix Paeoniae Alba 5-8 part, Herba Menthae 5-8 part, Herba Phyllanthi Urinariae 12-16 part, Fructus Arctii 5-8 part, Rhizoma Coptidis 8-12 part, Radix Glycyrrhizae 8-12 part, Radix Notoginseng 8-12 part, Fructus Crataegi 18-22 part, Radix Bupleuri 8-12 part, Radix Salviae Miltiorrhizae 5-8 part, Galla Chinensis 5-8 part, Rhizoma Alismatis 8-12 part, Rhizoma Atractylodis 28-32 part.
The present invention treats the Chinese medicine composition of oral ulcer, obtained by the raw material of following preferred weight part: Radix Scutellariae 15 parts, Flos Lonicerae 6 parts, Pericarpium Granati 10 parts, Calyx Seu Fructus Physalis 10 parts, Radix Asparagi 10 parts, Herba Taraxaci 20 parts, Periostracum Cicadae 20 parts, 10 parts, Folium Nelumbinis, the Radix Paeoniae Alba 5 parts, Herba Menthae 5 parts, Herba Phyllanthi Urinariae 15 parts, Fructus Arctii 7 parts, Rhizoma Coptidis 10 parts, 10 parts, Radix Glycyrrhizae, Radix Notoginseng 10 parts, Fructus Crataegi 20 parts, Radix Bupleuri 10 parts, Radix Salviae Miltiorrhizae 6 parts, Galla Chinensis 5 parts, Rhizoma Alismatis 10 parts, Rhizoma Atractylodis 30 parts.
Radix Scutellariae another name Radix Scutellariae, tsuchikane tea root, be labiate, be used as medicine with root.Have heat clearing and damp drying, removing heat from blood is antiabortive, detoxicating functions.Cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.Rhizoma Atractylodis have drying damp and strengthening spleen, expelling wind and cold, effect of improving eyesight, and for dampness taste, distension and fullness in the abdomen, cold-damp leucorrhea, damp-warm diseases and damp invasion of lower energizer, foot swelling and pain in knee, flaccidity are soft unable.Control dampness taste, and see the thick disease such as turbid of distension and fullness in the abdomen, inappetence, fatigue and weakness, white and greasy fur, the normal and compatibility such as Cortex Magnoliae Officinalis, Pericarpium Citri Reticulatae is applied.
Flos Lonicerae is Caprifoliaceae woodbine Radix Ophiopogonis and congener dry flower or is with the flower just opened.Flos Lonicerae is described as the good medicine of heat-clearing and toxic substances removing from ancient times.Its property sweet cold fragrance, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil.Flos Lonicerae can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile disease, as fever of the body, dermexanthesis, sends out the cards such as speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain, equal Be very effective.Modern medicine shows, Flos Lonicerae is containing cyclohexanhexanol, flavonoid, inositol, Saponin and tannin etc., there is broad-spectrum antibacterial action, all there is stronger inhibitory action to the various pathogens such as staphylococcus aureus, dysentery bacterium, also have inhibitory action to multiple pathogenic microorganisms such as leptospira, influenza virus and pathogenic moulds.In addition, Flos Lonicerae also has obvious antiinflammatory and refrigeration function, and honeysuckle flower water and steeping in wine liquid have obvious lethal effect to tentative tumor cell.
Pericarpium Granati system Uygur medicine medicinal herbs most in use are the dried fruit of Punicaceae plant Punica granatum L. (punicagranatum L.)
Skin.Pericarpium Granati acid, puckery, be mainly used in chronic diarrhea, the card such as hemorrhage in China's traditional medicine.Polyphenol in Pericarpium Granati is the general name that a class has polyphenol hydroxyl compound, comprise the multiple compounds such as elladitannin, Galla Turcica (Galla Helepensis) tannin, ellagic acid, gallic acid, content is about the 10%-20% of its dry mass, has antibacterial, multiple physiology and the pharmacologically active such as antioxidation, defying age, anti-cancer and cancer-preventing, blood pressure lowering and prevention cardiovascular and cerebrovascular disease.
Calyx Seu Fructus Physalis is the dry Constellation calyx of plant of Solanaceae Calyx seu fructus physalis Physalis alkekengiL.var.franchetii (mast.) Makino or the Constellation calyx of band fruit.Its nature and flavor are sweet, sour, cold, there is heat clearing away, removing toxic substances, diuresis, clears phlegm, be used for the treatment of that pharyngalgia is silent, phlegm-heat cough, dysuria, external treatment pemphigus, eczema etc.Calyx Seu Fructus Physalis contains several large class chemical compositions such as lactone, flavonoid, terpenoid, alkaloids, sterols, amino acids and inorganic elements, as physalin compounds, luteolin etc., has clear and definite antibacterial, antiinflammatory, antiallergic and analgesia isoreactivity.Be usually used in acute, suppurative tonsillitis and laryngopharynx swelling and pain clinically.
Radix Asparagi is the tuber that Liliaceae asparagus plant has the spindle meat that clusters, and has replenishing YIN and removing heat, moisturizes the effect of promoting the production of body fluid.Cure mainly that pulmonary tuberculosis, bronchitis, diphtheria, pertussis, dryness of the mouth and throat, calentura are thirsty, the disease such as diabetes, constipation due to dry stool; Sore swollen toxin is controlled in external, venom.Modern medicine confirms that Radix Asparagi is containing asparagine, β-sitosterol, steroid saponin, and the composition such as phlegmatic temperament, furfural derivatives, it has significant antibacterial action, antitumor action and Antitussive and Expectorant Effect.
Dandelion Compositae herbaceos perennial.Containing multiple health-nutrition compositions such as taraxol, taraxacin, choline, organic acid, inulin in Herba Taraxaci plant, there are the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting.Record according to Compendium of Material Medica, the flat sweet and slightly bitter taste of its property, has heat-clearing and toxic substances removing, dispersing swelling and dissipating binds and galgctogogue action, very effective to treatment mastitis.No matter decoct juice oral, or smash to mud external application, all there is effect.In addition, Herba Taraxaci also has the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting, is widely used in clinical.
Periostracum Cicadae has dispelling wind and heat pathogens effect, often coordinates the equivalent use of Herba Menthae for wind heat table disease; Rubella pruritus is also had to the function of dispelling wind for relieving itching.Clinically cure mainly loose wind heat, lung qi dispersing, determine convulsion.Control affection due to external wind and heat, cough hoarseness, measles is sent out not smooth thoroughly, rubella pruritus, pediatric epilepsy scared, conjunctival congestion, cataracta, furuncle swelling toxin, the diseases such as tetanus.Modern medicine proves that Periostracum Cicadae has cental system and extensively suppresses usefulness, and has stronger immunosuppressant and anti-allergic effects.
Folium Nelumbinis is the leaf of nymphaeaceae plant lotus, and national most area is all produced.Folium Nelumbinis bitter in the mouth is pungent micro-puckery, cool in nature, and GUIXIN, liver, spleen channel, delicate fragrance rise loose; There is summer heat relieving and dampness eliminating, spleen invigorating yang invigorating, effect of dissipating blood stasis hemostasis.Cure mainly summer-heat excessive thirst, have a headache dizzy, edema, lack of appetite abdominal distention, dysentery, leucorrhea, proctoptosis, spit blood, epistaxis, spitting of blood, has blood in stool, metrorrhagia, post partum longtime lochia, traumatic blood stasis.Modern medicine confirms that Folium Nelumbinis all has certain therapeutic effect to diarrhoea, edema, hyperlipidemia and dermatosis.
The Radix Paeoniae Alba enters liver, spleen channel, has nourishing blood to suppress the hyperactive liver, pain relieving in slow, and yin fluid astringing receives effect of antiperspirant.Cure mainly the breast abdomen pain over the hypochondriac region, dysentery is suffered from abdominal pain, and spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, is with inferior disease.
Herba Menthae is one of Chinese conventional Chinese medicine.It is pungent cool property sweating antipyretic medicine, controls influenza, the disease such as headache, conjunctival congestion, fever of the body, throat, dental bed swell and ache.External can training the mind dysmenorrhoea, skin pruritus, erythra and eczema etc.The Herba Menthae of light friend Greenhouse Fruit Picking is again the fresh vegetable on dining table in the Spring Festival.Clearly good to eat.Usually with Herba Menthae for tea, clear away heart-fire improving eyesight.Modern medicine proves that Herba Menthae has very strong antiviral and antibacterial activity, and has certain antiallergic activity.
Herba Phyllanthi Urinariae is dicotyledon medicine euphorbia plant Cacumen Securinegae Suffruticosae or whole herb with root, has effect of calming liver and clearing heat, promoting diuresis to remove toxic substance, cures mainly enteritis clinically, the diseases such as dysentery, infectious hepatitis, oedema due to nephritis, innominate toxic swelling.Prior art proves that Herba Phyllanthi Urinariae has the effect of antiviral and antiinflammatory, and can suppress to a certain extent and reverse hepatic fibrosis.
Fructus Arctii is called as Fructus Arctii, and originate in China, Compendium of Material Medica claims " Fructus Arctii " (also known as Fructus Arctii) is 2 years raw herbaceous plant, and its son, its root all can be used as medicine, also edible, and " Bencao Jingshu " is called " loose wind is except thermal detoxification three key medicine ".Prior art proves that it has significant anti-bacteria and anti-virus effect, and the immunocompetence of the remarkable enhancing body of energy.Herba Phyllanthi Urinariae and Fructus Arctii two kinds of medicines are used in conjunction, can the immunocompetence of remarkable enhancing body.
Rhizoma Coptidis has another name called Wang Lian, and bitter in the mouth is cold in nature, nontoxic.GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel.There is heat clearing and damp drying, effect of eliminating fire and detoxication.For damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood tells nosebleed, conjunctival congestion, and toothache, quenches one's thirst, carbuncle furuncle; External treatment eczema, eczema, auditory meatus is suppurated.It is burning hot that Rhizoma Coptidis (processed with wine) is apt to the clear part of the body cavity above the diaphragm housing the heart and lungs.For conjunctival congestion, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.Tie mutually for cold and heat, damp and hot middle resistance, feeling of fullness is vomitted.Cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting.For incoordination between the liver and stomach, vomiting acid regurgitation.According to clinical report, Berberine in treating cervical spondylosis can make blood glucose obviously reduce.Boiling Rhizoma Coptidis can reduce the blood glucose of normal mouse and alloxan cervical spondylosis Mus.Experiment shows that the blood sugar lowering mechanism of berberine does not affect secretion and the release of insulin, does not also affect number and the affinity of Hepatocyte Insulin, but by suppressing gluconeogenesis and promoting glycolysis and produce blood sugar reducing function.
Chinese medicine composition Rhizoma Alismatis of the present invention and Calyx Seu Fructus Physalis can play dispelling phlegm and eliminating dampness, protect the liver jaundice eliminating.Radix Notoginseng, Radix Asparagi blood circulation promoting and blood stasis dispelling, removing mass dredging collateral; Fructus Aurantii and Fructus Crataegi can promoting digestion and invigorating the stomach, elimination distension and fullness in the abdomen symptoms.Radix Bupleuri and the tender enough heat clearing and damp drying of Radix Scutellariae, eliminating stagnation are induced sweat.The Radix Paeoniae Alba and Radix Salviae Miltiorrhizae can nourishing blood to suppress the hyperactive liver, nourishing blood to tranquillize the mind.Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription.All medicines share, and play effect of dispersing the stagnated live-QI to relieve the stagnation of QI, spleen invigorating food stagnation removing, activating blood circulation to dissipate blood stasis, dispelling phlegm and eliminating dampness altogether.
The method preparing Chinese medicine composition of the present invention is as follows: each medical material getting above-mentioned components by weight percent, adds the 5-15 water doubly of medical material gross weight, soaks 10-60 minute, decoct 1-4 time, each 1-3 hour, filter, merging filtrate, filtrate reduced in volume is to the extractum of relative density 1.05-1.25, and this relative density is testing result at 60 c, and adding ethanol to alcohol content is 60-90%(v/v), leave standstill 24 hours, get supernatant, reclaim ethanol to concentrated, to obtain final product.The above-mentioned Chinese medicine composition of the present invention can be prepared into pharmaceutical preparation conventional clinically further, is preferably mixture, tablet, capsule.
The present invention also discloses a kind of purposes of above-mentioned Chinese medicine composition, i.e. the purposes of above-mentioned Chinese medicine composition in preparation treatment or preventing canker sore medicine.Chinese medicine composition of the present invention, when treating oral ulcer, has embodied significant antiinflammatory, antibacterial and analgesic effect, and has obtained good clinical therapeutic efficacy.
Detailed description of the invention
Further describe the present invention below by way of detailed description of the invention, but the present invention is not limited only to following examples.Within the scope of the invention or not departing from content of the present invention, spirit and scope, Chinese medicine composition of the present invention is suitably improved, replaces the identical component of effect, will become apparent to those skilled in the art that they are all deemed to be included within scope of the present invention.
part I, the preparation of Chinese medicine composition of the present invention
embodiment 1 Chinese medicine composition mixture of the present invention
Prescription: Radix Scutellariae 15 parts, Flos Lonicerae 6 parts, Pericarpium Granati 10 parts, Calyx Seu Fructus Physalis 10 parts, Radix Asparagi 10 parts, Herba Taraxaci 20 parts, Periostracum Cicadae 20 parts, 10 parts, Folium Nelumbinis, the Radix Paeoniae Alba 5 parts, Herba Menthae 5 parts, Herba Phyllanthi Urinariae 15 parts, Fructus Arctii 7 parts, Rhizoma Coptidis 10 parts, 10 parts, Radix Glycyrrhizae, Radix Notoginseng 10 parts, Fructus Crataegi 20 parts, Radix Bupleuri 10 parts, Radix Salviae Miltiorrhizae 6 parts, Galla Chinensis 5 parts, Rhizoma Alismatis 10 parts, Rhizoma Atractylodis 30 parts.
Preparation method: each medical material getting above-mentioned components by weight percent, adds the water of 5 times of medical material gross weight, soaks 30 minutes, decoct 2 times, each 2 hours, filter, merging filtrate, filtrate reduced in volume is to the extractum of relative density 1.1, and this relative density is testing result at 60 c, adding ethanol to alcohol content is 75%(v/v), leave standstill 24 hours, get supernatant, recovery ethanol concentrates, and adds water to 1000ml, stirs evenly, subpackage, flowing steam sterilization 35min, obtains mixture.
embodiment 2 Chinese medicine composition mixture of the present invention
Prescription: Radix Scutellariae 12 parts, Flos Lonicerae 5 parts, Pericarpium Granati 8 parts, Calyx Seu Fructus Physalis 8 parts, Radix Asparagi 8 parts, Herba Taraxaci 18 parts, Periostracum Cicadae 18 parts, 8 parts, Folium Nelumbinis, the Radix Paeoniae Alba 5 parts, Herba Menthae 5 parts, Herba Phyllanthi Urinariae 12 parts, Fructus Arctii 5 parts, Rhizoma Coptidis 8 parts, 8 parts, Radix Glycyrrhizae, Radix Notoginseng 8 parts, Fructus Crataegi 18 parts, Radix Bupleuri 8 parts, Radix Salviae Miltiorrhizae 5 parts, Galla Chinensis 5 parts, Rhizoma Alismatis 8 parts, Rhizoma Atractylodis 28 parts.
Preparation method: with embodiment 1.
embodiment 3 Chinese medicine composition mixture of the present invention
Prescription: Radix Scutellariae 16 parts, Flos Lonicerae 8 parts, Pericarpium Granati 12 parts, Calyx Seu Fructus Physalis 12 parts, Radix Asparagi 12 parts, Herba Taraxaci 22 parts, Periostracum Cicadae 22 parts, 12 parts, Folium Nelumbinis, the Radix Paeoniae Alba 8 parts, Herba Menthae 8 parts, Herba Phyllanthi Urinariae 16 parts, Fructus Arctii 8 parts, Rhizoma Coptidis 12 parts, 12 parts, Radix Glycyrrhizae, Radix Notoginseng 12 parts, Fructus Crataegi 22 parts, Radix Bupleuri 12 parts, Radix Salviae Miltiorrhizae 8 parts, Galla Chinensis 8 parts, Rhizoma Alismatis 12 parts, Rhizoma Atractylodis 32 parts.
Preparation method: with embodiment 1.
embodiment 4 Chinese medicine composition tablet of the present invention
Prescription: Radix Scutellariae 15 parts, Flos Lonicerae 6 parts, Pericarpium Granati 10 parts, Calyx Seu Fructus Physalis 10 parts, Radix Asparagi 10 parts, Herba Taraxaci 20 parts, Periostracum Cicadae 20 parts, 10 parts, Folium Nelumbinis, the Radix Paeoniae Alba 5 parts, Herba Menthae 5 parts, Herba Phyllanthi Urinariae 15 parts, Fructus Arctii 7 parts, Rhizoma Coptidis 10 parts, 10 parts, Radix Glycyrrhizae, Radix Notoginseng 10 parts, Fructus Crataegi 20 parts, Radix Bupleuri 10 parts, Radix Salviae Miltiorrhizae 6 parts, Galla Chinensis 5 parts, Rhizoma Alismatis 10 parts, Rhizoma Atractylodis 30 parts.
Preparation method: each medical material getting above-mentioned components by weight percent, adds the water of 10 times of medical material gross weight, soaks 60 minutes, decoct 1 time, each 1 hour, filter, merging filtrate, filtrate reduced in volume is to the extractum of relative density 1.25, and this relative density is testing result at 60 c, adding ethanol to alcohol content is 80%(v/v), leave standstill 24 hours, get supernatant, reclaim ethanol to concentrated, vacuum drying, granulation, tabletting and get final product.
embodiment 5 Chinese medicinal composition capsules of the present invention
Prescription: Radix Scutellariae 15 parts, Flos Lonicerae 6 parts, Pericarpium Granati 10 parts, Calyx Seu Fructus Physalis 10 parts, Radix Asparagi 10 parts, Herba Taraxaci 20 parts, Periostracum Cicadae 20 parts, 10 parts, Folium Nelumbinis, the Radix Paeoniae Alba 5 parts, Herba Menthae 5 parts, Herba Phyllanthi Urinariae 15 parts, Fructus Arctii 7 parts, Rhizoma Coptidis 10 parts, 10 parts, Radix Glycyrrhizae, Radix Notoginseng 10 parts, Fructus Crataegi 20 parts, Radix Bupleuri 10 parts, Radix Salviae Miltiorrhizae 6 parts, Galla Chinensis 5 parts, Rhizoma Alismatis 10 parts, Rhizoma Atractylodis 30 parts.
Preparation method: each medical material getting above-mentioned components by weight percent, adds the water of 8 times of medical material gross weight, soaks 45 minutes, decoct 4 times, each 1.5 hours, filter, merging filtrate, filtrate reduced in volume is to the extractum of relative density 1.2, and this relative density is testing result at 60 c, adding ethanol to alcohol content is 90%(v/v), leave standstill 24 hours, get supernatant, reclaim ethanol to concentrated, vacuum drying, granulates, encapsulated and get final product.
part II, the pharmacodynamics of Chinese medicine composition of the present invention is investigated
the antiinflammatory action of embodiment 4 Chinese medicine composition xylol of the present invention induced mice inflammatory model
Hydrocortisone has stronger anti-inflammatory activity, be synthetic be also naturally occurring glucocorticoid, after hydrocortisone enters cell, activate cytoplasmic receptor, nucleus is entered after allosteric, be combined with DNA response element, cause suppression or the induction of genetic transcription, the expression of inflammation related proteins is changed.
experimental technique:
40 KM mices, male and female half and half, body weight 25-30 gram, four groups are divided at random by body weight, i.e. dosage group in model control group, combination group high dose group (1.2% pentoxifylline glycerite), compositions, compositions low dose group (2% carbazochrome sodium sulfonate glycerite), positive controls, often organize 10, each administration group mice gives following medicine respectively:
Model control group: gavage gives the normal saline of same volume;
Positive controls: gavage gives the hydrocortisone drug solution of 5mg/kg;
Compositions high dose group: gavage gives 50mg/kg embodiment 1 Chinese medicine composition, and Chinese medicine composition presses crude drug gauge;
Dosage group in compositions: gavage gives 20 mg/kg embodiment 1 Chinese medicine compositions, and Chinese medicine composition presses crude drug gauge;
Compositions low dose group: gavage gives 5 mg/kg embodiment 1 Chinese medicine compositions, and Chinese medicine composition presses crude drug gauge;
Each administration group is administered once every day, successive administration 7d.Get dimethylbenzene 20 μ l after last administration, be applied to each mouse right ear.Get after 30 minutes and be respectively evenly applied to mouse right ear respectively by reagent 0.03 ml, auris dextra is put to death after 3.5 hours to dimethylbenzene, measures each group of mice left and right ear method of double differences.Ear method of double differences assay method: place cuts two ears along auricle baseline, lays auricle with 8mm card punch at the same position of left and right ear, weighs, the ear method of double differences=auris dextra sheet weight-left auricle weight.The ear method of double differences can be used as evaluates antiinflammatory index.
experimental result:
Measure the antiphlogistic effects that each experimental mice ear method of double differences evaluates each medication group medicine.Measurement result is in table 1.
Table 1 each experimental mice ear method of double differences compares
Compare with model control group,
* p< 0.05; Compare with model control group,
* p< 0.01; Compare with positive controls,
# p< 0.05; Compare with positive controls,
## p< 0.01.
Result display gives positive drug and Chinese medicine composition of the present invention all can obtain significant antiphlogistic effects, wherein the antiphlogistic effects of Chinese medicine composition high dose group of the present invention and positive controls have pole significant difference, Chinese medicine composition low dose group and positive controls have significant difference, and this shows that the high dose group of Chinese medicine composition of the present invention and the antiphlogistic effects of low dose group significantly will be better than positive controls.
the antibacterial experiment in vitro of embodiment 5 Chinese medicine composition of the present invention
The strain that this experiment uses and culture medium are respectively staphylococcus epidermidis (32209), beta hemolytic streptococcus (32210), colon bacillus (44102), proteus vulgaris (49101), Streptococcus mutans (50001), Diplococcus pneumoniae (51252), staphylococcus aureus [26111 (5)], Candida albicans (58529-4), nutrient agar (in tired pharmaceutical biological product calibrating institute); Opacity tube (Chinese bacterial turbidity standard, Chinese medicine biological combinations calibrating institute).
Antibacterial experiment in vitro: 1. prepare pastille agar plate.Tested material is pressed the culture medium of doubling dilution preparation different pharmaceutical concentration, heating, modulation is required pH value separately, and 121 DEG C of sterilizing 30 min, are chilled to 45 DEG C.Pour into into aseptic flat board (plate d=65 mm, 10 ml/ plates), finally make the extension rate containing tested material stock solution (2g/ml) in culture medium be respectively l:2,1:4 ... 1:1024.Do respective blank culture medium simultaneously.2. inoculate.Get the bacterium in young age reaching the third generation, be the bacterium liquid to be checked of 108 cfu/ml respectively by standard opacity tube corrected concentrations with 0.9% sodium chloride solution, be inoculated in the respective pastille agar surface of different doubling dilution respectively, Simultaneous vaccination parallel point 3 point, make at often containing 2 one bacterium liquid.The liquid-drop diameter of each vaccination is 5-8 mm.After point to be seeded drying, upset is dull and stereotyped cultivates 20 h observed results (simultaneously doing blank) in 36 DEG C of constant incubators.3. criterion.Not occur that minimum dilution tested material concentration on the agar plate of bacterium colony is for its minimum inhibitory concentration (MIC).
In this experiment, the minimal inhibitory concentration of Chinese medicine composition to each pathogenic strain is as shown in table 2.
Table 2 Chinese medicine composition of the present invention is to the minimal inhibitory concentration of common oral pathogenic strain
Result shows, and Chinese medicine composition of the present invention all has good fungistatic effect to most oral cavity pathogen, and this, for being the most easily subject to the oral cavity of extraneous infection, has very important therapeutic potential.
embodiment 6 Chinese medicine composition of the present invention affects mouse hot-plate analgesic
1 mouse hot-plate pain model makes
About body weight 20g female mice is placed on hot plate dolorimeter, screen qualified ♀ mice 90 (to lick metapedes time < 5 s or > 30 s to throw aside, scald for preventing foot, also deadline should be established, be generally 60s), the incubation period of metapedes reaction is added for threshold of pain index with mice, get 72 jennies, be divided into 6 groups, 12/group, i.e. model group, cydiodine, Huanglianshangqing Tablet group, Chinese medicine composition of the present invention (obtaining by Chinese medicine composition prescription described in embodiment 1 and preparation technology) low dose group, middle dosage group and high dose group.Each group is gastric infusion, and 1 times/day, dosage is as shown in table 10, and after successive administration 14d, after measuring last medicine, 60 min, 120 min add the time of foot reaction.
chinese medicine composition of the present invention is on the impact of licking the foot response latency in mouse hot-plate analgesic experiment
Mice licks foot response latency time showing, compare with model group, after administration after 1h and 2h, the incubation period that the mice of each administration group of Chinese medicine composition of the present invention adds foot reaction is all greater than model group, there is dose-dependence, and be significantly greater than the incubation period of high dose group model group (
p< 0.05).What illustrate that this Chinese medicine composition of the present invention can extend that mice stimulates hot plate adds the sufficient response latency, has certain analgesic activity.Under same experiment condition, positive control drug cydiodine group (
p< 0.05) and Huanglianshangqing Tablet group (1h,
p< 0.05; 2h,
p< 0.01) also extend the incubation period that mice adds foot reaction, there is certain analgesic activity, but its analgesic effect and model group there was no significant difference.The results are shown in Table 3.
Table 3 mice lick foot response latency timetable (
)
Note: compare with model group, *
p< 0.05, * *
p< 0.01.
embodiment 7 Chinese medicine composition of the present invention is to the clinical therapeutic efficacy of hemorrhagic oral ulcer
1, clinical data
This group observe case 50 example, wherein male 38 example, women 12 example, age 15-45 year, 32 years old mean age.
diagnostic criteria
There is single or multiple circle of differing in size or oval ulcer in the mucosa at the places such as the lip in oral cavity, cheek, soft palate or gums, ulcer surface diameter is 1-10mm, greyish white or the yellow pseudomembrane of surface coverage, concavity, clear border, surrounding mucosa is red and micro-swollen, local, ulcer place obviously causalgia, severe patient also can affect appetite, lymph enlargement, causes very big inconvenience to diet.
therapeutic Method
For oral administration from intending oral ulcer mixture.Prescription: Radix Scutellariae 15 parts, Flos Lonicerae 6 parts, Pericarpium Granati 10 parts, Calyx Seu Fructus Physalis 10 parts, Radix Asparagi 10 parts, Herba Taraxaci 20 parts, Periostracum Cicadae 20 parts, 10 parts, Folium Nelumbinis, the Radix Paeoniae Alba 5 parts, Herba Menthae 5 parts, Herba Phyllanthi Urinariae 15 parts, Fructus Arctii 7 parts, Rhizoma Coptidis 10 parts, 10 parts, Radix Glycyrrhizae, Radix Notoginseng 10 parts, Fructus Crataegi 20 parts, Radix Bupleuri 10 parts, Radix Salviae Miltiorrhizae 6 parts, Galla Chinensis 5 parts, Rhizoma Alismatis 10 parts, Rhizoma Atractylodis 30 parts.Be decocted in water for oral dose, every day 1 dose, 2 times/d, successive administration 4 days.
criterion of therapeutical effect
Cure: in oral cavity, ulcer surface disappears, without redness, without causalgia, lymph is normal.
Take a turn for the better: ulcer surface number reduces, and ulcer surface reduced, causalgia alleviates, and lymph swells and ache and alleviates.
Invalid: ulcer surface number, ulcer surface diameter, causalgia sense are all not improved.
curative effect
In 50 examples, cure 41 examples, account for 82 %; Take a turn for the better 9 examples, accounts for 18 %.
The embodiment of the present invention 4 shows, give positive drug and Chinese medicine composition of the present invention all can obtain significant antiphlogistic effects, wherein the antiphlogistic effects of Chinese medicine composition high dose group of the present invention and positive controls have pole significant difference, Chinese medicine composition low dose group and positive controls have significant difference, and this shows that the high dose group of Chinese medicine composition of the present invention and the antiphlogistic effects of low dose group significantly will be better than positive controls.The embodiment of the present invention 5 shows, Chinese medicine composition of the present invention all has good fungistatic effect to most oral cavity pathogen, and this, for being the most easily subject to the oral cavity of extraneous infection, has very important therapeutic potential.Mice in the embodiment of the present invention 6 licks foot response latency time showing, compare with model group, after administration after 1h and 2h, the incubation period that the mice of each administration group of Chinese medicine composition of the present invention adds foot reaction is all greater than model group, there is dose-dependence, and be significantly greater than the incubation period of high dose group model group (
p< 0.05).What illustrate that this Chinese medicine composition of the present invention can extend that mice stimulates hot plate adds the sufficient response latency, has certain analgesic activity.Under same experiment condition, positive control drug cydiodine group (
p< 0.05) and Huanglianshangqing Tablet group (1h,
p< 0.05; 2h,
p< 0.01) also extend the incubation period that mice adds foot reaction, there is certain analgesic activity, but its analgesic effect and model group there was no significant difference.The embodiment of the present invention 7 shows, and Chinese medicine composition of the present invention is 100% to the clinical effective rate of oral ulcer, and wherein cure rate is 82%, and improvement rate is 18%, has certain treatment advantage.
In a word, the present invention compared with prior art, has the activity for the treatment of oral ulcer very well, and can the present invention be pure Chinese medicinal preparation, to human body particularly to liver avirulence.When the present invention uses as Aphthasol thing, drug effect is comprehensive, has not only embodied significant antiinflammatory bactericidal effect, and significantly can alleviate canker sore pain; The immunocompetence of body can be regulated while eliminating oral ulcer symptom, oral ulcer conditions of patients can be made rapidly to take a turn for the better, play the therapeutic effect for the treatment of both the principal and secondary aspects of a disease; And Chinese medicine composition preparation of the present invention is simple, and substantially nontoxic, raw material is easy to get, and is suitable for popular use, has good application prospect.