CN102911982A - Application of bananas in biocellulose fermentation media - Google Patents
Application of bananas in biocellulose fermentation media Download PDFInfo
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- CN102911982A CN102911982A CN2012104810865A CN201210481086A CN102911982A CN 102911982 A CN102911982 A CN 102911982A CN 2012104810865 A CN2012104810865 A CN 2012104810865A CN 201210481086 A CN201210481086 A CN 201210481086A CN 102911982 A CN102911982 A CN 102911982A
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Abstract
The invention relates to application of tropical fruit bananas in biocellulose fermentation media. The invention has the following beneficial effects: the application range of the bananas is expanded, and the subsidiary production values of the bananas are increased; meanwhile, biocellulose products with different tastes and impressions are obtained; and by adding special nitrogen sources, higher biocellulose yield and better characteristics are obtained, and the application range of tropical fruit papayas is expanded. In short, the invention relates to a more economical and effective biocellulose production method.
Description
Technical field
The present invention relates to a kind of production method of biology cellulose.
Background technology
Biology cellulose (biocellulose, claim again bacteria cellulose, be called for short BC) typically refer under different condition, by synthetic cellulosic general designation such as certain microorganism in acetic acid Pseudomonas (Acetobacter), Agrobacterium (Agrobacterium), rhizobium (Rhizobium) and the Sarcina (Sarcina) etc.Compare with natural cellulose, it has the advantageous properties such as high purity, high-crystallinity, high-polymerization degree, high retentiveness and high rehydration.Be widely used in every field.The production of biology cellulose, normally with microbial inoculants such as acetic acid Pseudomonas in the substratum that is rich in the nutritive ingredients such as nitrogenous source and carbon source, in 20-30 ℃ of lower static fermentation 5-10 days, can obtain translucent biology cellulose film on the surface of liquid culture medium.
The fermentation to produce biological Mierocrystalline cellulose usually adopts Sucus Cocois as the main component of substratum at present, although it is with low cost, be easy to use, but its source is limitation comparatively, the supply less, along with the Application Areas of biology cellulose film product constantly enlarges, demand is also increasing, and the industrial scale of expanding day needs the raw material of more suitable for producing; And, need to provide physical strength larger biology cellulose product in some field, and adopt Sucus Cocois can not satisfy its demand as the product that fermention medium obtains.
Summary of the invention
The source that the purpose of this invention is to provide more biology cellulose fermentation substrate, mainly be utilize the most common plantation of tropical area and production scale all very large tropical fruit as the source of fermentation substrate.Both expand the scope that tropical fruit is used, increased its earning rate; Can supply in large quantity again the biology cellulose fermentation required.
The present invention mainly provides the purposes of banana in the biology cellulose fermention medium, contains the banana fruit juice of weight percent 50 ~ 90% in the described fermention medium, and described banana fruit juice sugar degree is at more than 10% of weight percent.
Banana is a kind of typical tropical fruit, and its fruits nutrition is abundant, and the per 100 gram pulp carbohydrate containing of banana 20 grams, protein 1.2 grams, fat 0.6 gram in addition, also contain various trace elements and VITAMIN.Carbohydrate is mainly starchiness in the banana before ripe, but starch transforms saccharogenesis substantially fully in the banana after the maturation.During the fruit fully matured, starch is down to 1%～2% from 20%～23%, and soluble sugar then increases to 20% from 1%.Wherein the ratio of glucose, fructose, sucrose is 20:15:65, can be utilized by biology cellulose fermentative production bacterium fully, so with it as fermenting carbon source, be extraordinary carbon source source.And VITAMIN and other inorganic elementss contained in the banana also can utilize for biology cellulose fermentative production bacteria growing, can improve better its fermentation efficiency.For the ease of using and prevent the banana juice browning, after the present invention removes the peel the fully matured banana, the hot water and the citric acid that accounts for pulp weight 0.5% that add 85 ℃, making beating was processed 1 minute ~ 5 minutes, make banana fruit juice after refiltering, concentrating, with its main component as fermention medium, after regulating the pH value, be directly used in the fermentation to produce biological Mierocrystalline cellulose; Also can it be carried out suitable allotment, as add an amount of nitrogenous source or inorganic elements etc. again according to the physiological property of different fermentations bacterial classification, be used further to fermentation.
Containing polyphenoloxidase in the banana, is the principal element that causes the bananas juice brown stain.The polyphenol oxidase enzyme heat stability is high, be difficult for inactivation, the pyroprocessing of employing more than 75 ℃ be its enzymic activity of passivation effectively, prevent brown stain, add again citric acid and make color retention, can effectively control the brown stain of banana fruit juice, make it have good impression, also be beneficial to the utilization of biology cellulose fermented bacterium.
Banana fruit juice for fermention medium of the present invention can prepare in advance in the country of origin, is transported to the biology cellulose grown place again; But higher for movement requirement like this; After preferably banana being transported to the biology cellulose grown place, preparation is used again.
Banana fruit juice for fermention medium of the present invention directly uses after can being concentrated on request certain sugar degree, the biology cellulose of producing can be with light banana fragrance, simultaneously because banana itself also contains the Mierocrystalline cellulose of high level, when fermentation, can organically combine with the fibrous texture of biology cellulose, finer and close solid netted fibrous texture is provided, making the biology cellulose that obtains of fermentation have the more mouthfeel of toughness and special fragrance, also having improved the physical strength of biology cellulose; Use after also banana fruit juice can being removed pigment, impurity, the biology cellulose of producing is pure transparent, and mouthfeel is sharp and clear.
Although contain inorganic elements and sugar than horn of plenty in the banana fruit juice, the phenomenon that power is not enough, biology cellulose output is lower after can there be fermentation in the biology cellulose fermentation later stage.Find through research, major cause is the required nitrogenous source deficiency of fermented bacterium in the fermenting process.So, the present invention further provides and be suitable for the required nitrogenous source source of biology cellulose fermentative production, it is the vegetable protein peptone, described vegetable protein peptone is to add Fructus Chaenomelis extract hydrolysis gained in the vegetable-protein; Described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained behind the tannin precipitator method removal impurity.The preferred soybean protein of described vegetable-protein and/or gluten.Described vegetable protein peptone is to obtain after adding the Fructus Chaenomelis extract hydrolysis in the vegetable-protein and being dried to meal; Its molecular weight is about 2000.
The present invention adopts vegetable-protein nitrogenous source source as the biology cellulose fermented bacterium after the Fructus Chaenomelis extract hydrolysis, and it is required to nitrogenous source that it can fully satisfy fermented bacterium, improves fermentation efficiency, improves tunning output; Can also take full advantage of the characteristic that this tropical fruit of pawpaw is rich in papoid; Because vegetal dietary protein origin has not only been adopted in the preparation of described vegetable protein peptone, also adopted vegetal proteolytic enzyme source, make in the peptone protein decomposition efficiency higher, each seed amino acid and micromolecule polypeptide content are higher, and it is used that kind and character are more conducive to the fermentation to produce biological Mierocrystalline cellulose, more can improve fermentation efficiency and product output; And all be the food source, so that the food safety of the biology cellulose that fermentative production obtains is guaranteed.
On the basis of above-mentioned research, the invention provides a kind of biology cellulose fermention medium of excellence, it contains the banana fruit juice of weight percent 50 ~ 90%, and described banana fruit juice sugar degree is at more than 10% of weight percent; Also contain the vegetable protein peptone that vegetable-protein obtains through the Fructus Chaenomelis extract hydrolysis, described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained behind the tannin precipitator method removal impurity.
The biology cellulose output of utilizing above-mentioned fermention medium fermentation to obtain is made the fermention medium of main composition than Sucus Cocois biology cellulose output can improve more than 10%.And toughness and the physical strength of product also are improved to some extent.
Above-mentioned fermention medium, wherein said vegetable-protein are soybean protein and/or gluten.
Above-mentioned fermention medium, wherein said vegetable protein peptone are to obtain after adding the Fructus Chaenomelis extract hydrolysis in the vegetable-protein and being dried to meal; Its molecular weight is about 2000.
The pH value of above-mentioned fermention medium is 5.2-6.5.Can adopt citric acid or Glacial acetic acid to regulate.
The invention provides the application of tropical fruit banana on the biology cellulose fermention medium, enlarged the range of application of banana, increased its by-product value; Obtained simultaneously the biology cellulose product of different mouthfeels and impression; Obtain higher biology cellulose output and better characteristic through adding special nitrogenous source again, increased again the range of application of tropical fruit pawpaw.In general, be a kind of more cost-effective biology cellulose production method.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
Embodiment 1:
After the fresh full ripe banana peeling, adds 85 ℃ hot water and account for the citric acid of pulp weight 0.5%, making beating was processed 2 minutes, made sugar degree after refiltering, concentrating at 10% banana fruit juice of weight percent.Regulate pH5.8 with citric acid.The seed liquor of inoculation biology cellulose fermentative production bacterial classification, such as acetobacter xylinum, 30 ℃ were descended static shallow tray fermentation 5 days, obtained membranaceous biology cellulose on the top layer of substratum.Biology cellulose output is 6.2g/L.The Young's modulus of humidogene fibres element film is 7.04Gpa.Gained biology cellulose film is oyster white, news light banana fragrance arranged.
Embodiment 2:
After the fresh full ripe banana peeling, adds 85 ℃ hot water and account for the citric acid of pulp weight 0.5%, making beating was processed 2 minutes, made sugar degree after refiltering, concentrating at 12% banana fruit juice of weight percent.An amount of polygalacturonase hydrolysis, the banana fruit juice of comparatively being clarified will be added in the described banana fruit juice; Small molecular weight impurity is removed in ultrafiltration again; Regulate pH6.0 with citric acid.The seed liquor of inoculation biology cellulose fermentative production bacterial classification, such as acetobacter xylinum, 30 ℃ were descended static shallow tray fermentation 5 days, obtained membranaceous biology cellulose on the top layer of substratum.Biology cellulose output is 6.6g/L.The Young's modulus of humidogene fibres element film is 5.84Gpa.Gained biology cellulose film is clear, colorless.
Embodiment 3:
After the fresh full ripe banana peeling, adds 85 ℃ hot water and account for the citric acid of pulp weight 0.5%, making beating was processed 2 minutes, made sugar degree after refiltering, concentrating at 15% banana fruit juice of weight percent.
Gather immature papaya slurries, make Fructus Chaenomelis extract with filtering behind the tannin precipitator method removal impurity.Described Fructus Chaenomelis extract is added in the soybean protein, and water at normal temperature solution 1 day makes soy peptone, and crushed after being dried makes molecular weight and be about 2000 soy peptone.
Prepare fermention medium by following weight percent consumption:
The banana fruit juice of sugar degree 15%: 80%;
Soy peptone: 10%;
Regulate pH5.2 with Glacial acetic acid.
Water: surplus.
The seed liquor of inoculation biology cellulose fermentative production bacterial classification in the above-mentioned fermention medium for preparing, such as acetobacter xylinum, 30 ℃ of lower static shallow tray fermentations 5 days obtain membranaceous biology cellulose on the top layer of substratum.Biology cellulose output is 11.2g/L.The Young's modulus of humidogene fibres element film is 7.56Gpa.Gained biology cellulose film is oyster white, news light banana fragrance arranged.
Embodiment 4:
After the fresh full ripe banana peeling, adds 85 ℃ hot water and account for the citric acid of pulp weight 0.5%, making beating was processed 3 minutes, made sugar degree after refiltering, concentrating at 18% banana fruit juice of weight percent.An amount of polygalacturonase hydrolysis, the banana fruit juice that obtains clarifying will be added in the described banana fruit juice; Small molecular weight impurity is removed in ultrafiltration again.
Gather immature papaya slurries, make Fructus Chaenomelis extract with filtering behind the tannin precipitator method removal impurity.Described Fructus Chaenomelis extract is added in the gluten, and water at normal temperature solution 1 day makes the gluten peptone, and crushed after being dried makes molecular weight and be about 2000 gluten peptone.
Prepare fermention medium by following weight percent consumption:
The banana fruit juice of sugar degree 18%: 80%;
Gluten peptone: 10%;
Regulate pH5.2 with Glacial acetic acid.
Water: surplus.
The seed liquor of inoculation biology cellulose fermentative production bacterial classification in the above-mentioned fermention medium for preparing, such as acetobacter xylinum, 30 ℃ of lower static shallow tray fermentations 5 days obtain membranaceous biology cellulose on the top layer of substratum.Biology cellulose output is 10.5g/L.The Young's modulus of biology cellulose is 6.89Gpa.Gained biology cellulose film is clear, colorless.
Embodiment 5:
After the fresh full ripe banana peeling, adds 85 ℃ hot water and account for the citric acid of pulp weight 0.5%, making beating was processed 2 minutes, made sugar degree after refiltering, concentrating at 15% banana fruit juice of weight percent.
Gather immature papaya slurries, make Fructus Chaenomelis extract with filtering behind the tannin precipitator method removal impurity.Described Fructus Chaenomelis extract is added in the soybean protein, and water at normal temperature solution 1 day makes soy peptone, and crushed after being dried makes molecular weight and be about 2000 soy peptone.
Prepare fermention medium by following weight percent consumption:
The banana fruit juice of sugar degree 15%: 90%;
Soy peptone: 5%;
Regulate pH6.2 with Glacial acetic acid.
Water: surplus.
The seed liquor of inoculation biology cellulose fermentative production bacterial classification in the above-mentioned fermention medium for preparing, such as acetobacter xylinum, 30 ℃ of lower static shallow tray fermentations 5 days obtain membranaceous biology cellulose on the top layer of substratum.Biology cellulose output is 15.1g/L.The Young's modulus of biology cellulose is 8.54Gpa.Gained biology cellulose film is oyster white, news light banana fragrance arranged.
Embodiment 6:
After the fresh full ripe banana peeling, adds 85 ℃ hot water and account for the citric acid of pulp weight 0.5%, making beating was processed 2 minutes, made sugar degree after refiltering, concentrating at 20% banana fruit juice of weight percent.
Gather immature papaya slurries, make Fructus Chaenomelis extract with filtering behind the tannin precipitator method removal impurity.Described Fructus Chaenomelis extract is added in the soybean protein, and water at normal temperature solution 1 day makes soy peptone, and crushed after being dried makes molecular weight and be about 2000 soy peptone.
Prepare fermention medium by following weight percent consumption:
The banana fruit juice of sugar degree 20%: 70%;
Soy peptone: 10%;
Regulate pH6.2 with Glacial acetic acid.
Water: surplus.
The seed liquor of inoculation biology cellulose fermentative production bacterial classification in the above-mentioned fermention medium for preparing, such as acetobacter xylinum, 30 ℃ of lower static shallow tray fermentations 5 days obtain membranaceous biology cellulose on the top layer of substratum.Biology cellulose output is 14.0g/L.The Young's modulus of biology cellulose is 7.98Gpa.Gained biology cellulose film is oyster white, news light banana fragrance arranged.
Illustrate: the seed liquor of the fermented bacterium that above-described embodiment adopts is under the equal conditions, the activation of identical bacterial strain obtains.Gained fermentation results data are the mean value of repeatedly parallel test.
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. the purposes of banana in the biology cellulose fermention medium, it is characterized in that: contain the banana fruit juice of weight percent 50 ~ 90% in the described fermention medium, described banana fruit juice sugar degree is at more than 10% of weight percent.
2. purposes according to claim 1 is characterized in that: described banana fruit juice is for after the peeling of fully matured banana, adds 85 ℃ hot water and accounts for the citric acid of pulp weight 0.5%, and making beating was processed 1 minute ~ 5 minutes, made after refiltering, concentrating.
3. purposes according to claim 1 and 2 is characterized in that: also contain the vegetable protein peptone in the described fermention medium, described vegetable protein peptone is to add Fructus Chaenomelis extract hydrolysis gained in the vegetable-protein; Described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained behind the tannin precipitator method removal impurity.
4. purposes according to claim 3, it is characterized in that: described vegetable-protein is soybean protein and/or gluten.
5. purposes according to claim 3 is characterized in that: described vegetable protein peptone is to obtain after adding the Fructus Chaenomelis extract hydrolysis in the vegetable-protein and being dried to meal; Its molecular weight is about 2000.
6. biology cellulose fermention medium, it is characterized in that: contain the banana fruit juice of weight percent 50 ~ 90%, described banana fruit juice sugar degree is at more than 10% of weight percent; Also contain the vegetable protein peptone that vegetable-protein obtains through the Fructus Chaenomelis extract hydrolysis, described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained behind the tannin precipitator method removal impurity.
7. fermention medium according to claim 6, it is characterized in that: described vegetable-protein is soybean protein and/or gluten.
8. fermention medium according to claim 6 is characterized in that: described vegetable protein peptone is to obtain after adding the Fructus Chaenomelis extract hydrolysis in the vegetable-protein and being dried to meal; Its molecular weight is about 2000.
9. fermention medium according to claim 6 is characterized in that: the pH value of described substratum is 5.2-6.5.
10. fermention medium claimed in claim 6 is used for the fermentation to produce biological Mierocrystalline cellulose.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107802543A (en) * | 2017-12-05 | 2018-03-16 | 贵州四季常青药业有限公司 | A kind of shining skin Biofibre facial mask of Rosa roxburghii element and preparation method thereof |
| CN109832623A (en) * | 2019-04-29 | 2019-06-04 | 臻味之(烟台)食品科技有限公司 | A kind of nutrition fermented food |
| CN110051544A (en) * | 2019-04-07 | 2019-07-26 | 武汉理工大学 | Environment protection biological cellulose mask matrix and preparation method thereof |
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| CN101347152A (en) * | 2008-08-01 | 2009-01-21 | 内蒙古蒙牛乳业（集团）股份有限公司 | Milk and dairy food containing fruit granules and method for producing the same |
| CN101671708A (en) * | 2009-10-19 | 2010-03-17 | 华南理工大学 | Method for fermented-producing bacteria cellulose with pineapple peel juice by two-step method |
| CN101805765A (en) * | 2010-04-01 | 2010-08-18 | 东华大学 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347152A (en) * | 2008-08-01 | 2009-01-21 | 内蒙古蒙牛乳业（集团）股份有限公司 | Milk and dairy food containing fruit granules and method for producing the same |
| CN101671708A (en) * | 2009-10-19 | 2010-03-17 | 华南理工大学 | Method for fermented-producing bacteria cellulose with pineapple peel juice by two-step method |
| CN101805765A (en) * | 2010-04-01 | 2010-08-18 | 东华大学 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107802543A (en) * | 2017-12-05 | 2018-03-16 | 贵州四季常青药业有限公司 | A kind of shining skin Biofibre facial mask of Rosa roxburghii element and preparation method thereof |
| CN107802543B (en) * | 2017-12-05 | 2020-08-07 | 贵州四季常青药业有限公司 | Roxburgh rose element skin-refreshing biological fiber mask and preparation method thereof |
| CN110051544A (en) * | 2019-04-07 | 2019-07-26 | 武汉理工大学 | Environment protection biological cellulose mask matrix and preparation method thereof |
| CN109832623A (en) * | 2019-04-29 | 2019-06-04 | 臻味之(烟台)食品科技有限公司 | A kind of nutrition fermented food |
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