CN102895377B - Pharmaceutical composition for treating livestock respiratory diseases and method for preparing pharmaceutical composition for treating livestock respiratory diseases - Google Patents
Pharmaceutical composition for treating livestock respiratory diseases and method for preparing pharmaceutical composition for treating livestock respiratory diseases Download PDFInfo
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Abstract
The invention provides a pharmaceutical composition for treating livestock respiratory diseases and a method for preparing the pharmaceutical composition for treating livestock respiratory diseases. The pharmaceutical composition consists of exocarpium citri grandis extract, poria extract, radix glycyrrhizae extract and fumaric acid tiamulin, wherein the exocarpium citri grandis extract accounts for 8-15 parts by weight, the poria extract accounts for 2-10 parts by weight, the radix glycyrrhizae extract accounts for 1-5 parts by weight, the fumaric acid tiamulin accounts for 5-20 parts by weight, and the pharmaceutical composition is prepared through a mixing mode. According to the pharmaceutical composition for treating livestock respiratory diseases, traditional Chinese medicine and western medicine are reasonably compounded, so that the effective treatment drug for preventing livestock respiratory diseases is provided for the breeding industry; the pharmaceutical composition has the functions of relieving coughs and asthma, resisting bacteria and virus, improving the immunity of the organism and the like and treats both the manifestation and root causes, the curative process is shortened, and the occurrence probability of drug resistance is greatly reduced; and the method for preparing the pharmaceutical composition is simple, is convenient to use and meets the requirements of mass industrial production.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition and preparation method thereof, especially a kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease and preparation method thereof.
Background technology
Livestock and birds respiratory disease increased popularity and aggravation, the development of aquaculture in serious threat, and popular area and outburst number of times constantly expand especially in recent years, and especially some zoonosiss threaten the health of the mankind.
Livestock and birds respiratory disease reason is various, is generally divided into three aspects: one be non-spreading venereal diseases because of, comprise the factors such as the harmful gas in poultry house, stocking density be excessive; Two are spreading venereal diseasess because of, comprise antibacterial, virus, parasite; Three is immunosuppressive agents, comprises some viruses, immunosuppressant etc. that physics, chemical factor cause.
Why important livestock and birds respiratory disease is, is on the one hand that the fowl poultry kind of each age in days all can occur, and has a strong impact on fowl poultry kind fertility performance and product quality, and the economic loss caused thus is serious because this disease is present in each period in growth of animals or poultry stage; The M & M of this disease is all high on the other hand, and easy other disease of secondary, it is reported, pig farm sickness rate is 30% ~ 60%, and mortality rate reaches 5% ~ 30%, and the mortality rate of chicken house is 10% ~ 20%, if mixed infection Other diseases, mortality rate reaches more than 50%.So the medicine of exploitation effective treatment livestock and birds respiratory disease is most important.
The medicine of current treatment respiratory tract disease is based on antibiotic, but these antibiotics are mainly for pathogenic bacterium, this kind of medicine is often control for the moment, easy recurrence, and the respiratory symptoms such as cough, asthma are lost control of, therefore in order to control the respiratory symptoms such as cough, always need to extend medication, this too increases drug residue while having increased the weight of raiser's aquaculture cost, and antibacterial produces the danger of drug resistance, result in the therapeutic effect of antibiotic to such disease more and more undesirable.On the other hand, although Chinese medicine has the advantages that well alleviate respiratory symptom, because it takes effect slowly, take length, be usually limited to use.Visible, a kind of medicine that fundamentally can solve respiratory tract disease is provided, cause of disease can be controlled rapidly and well can alleviate respiratory symptom again, there is very important production practices meaning.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease.
Another technical problem to be solved by this invention is the preparation method of the pharmaceutical composition providing above-mentioned control livestock and birds respiratory disease.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin, by its weight parts Exocarpium Citri Grandis extract 8 ~ 15 parts, Poria extract 2 ~ 10 parts, Radix Glycyrrhizae extract 1 ~ 5 part, fumaric acid tiamulin 5 ~ 20 parts.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, by its weight parts Exocarpium Citri Grandis extract 9 ~ 12 parts, Poria extract 5 ~ 8 parts, Radix Glycyrrhizae extract 3 ~ 4 parts, fumaric acid tiamulin 8 ~ 13 parts.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, by its weight parts Exocarpium Citri Grandis extract 10 parts, Poria extract 7 parts, Radix Glycyrrhizae extract 4 parts, fumaric acid tiamulin 11 parts.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and solubility pharmaceutic adjuvant, wherein the percentage by weight of each component is Exocarpium Citri Grandis extract 8% ~ 15%, Poria extract 2% ~ 10%, Radix Glycyrrhizae extract 1% ~ 5%, fumaric acid tiamulin 5% ~ 20%, solubility pharmaceutic adjuvant polishing to 100%.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, the percentage by weight of each component is Exocarpium Citri Grandis extract 9 ~ 12%, Poria extract 5 ~ 8%, Radix Glycyrrhizae extract 3 ~ 4%, fumaric acid tiamulin 8 ~ 13%, solubility pharmaceutic adjuvant polishing to 100%.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, the percentage by weight of each component is Exocarpium Citri Grandis extract 10%, Poria extract 7%, Radix Glycyrrhizae extract 4%, fumaric acid tiamulin 11%, solubility pharmaceutic adjuvant polishing to 100%.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, described Exocarpium Citri Grandis extract is obtained by water boiling and extraction technique.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, the water boiling and extraction technique of described Exocarpium Citri Grandis extract comprises the steps:
(1) Exocarpium Citri Grandis raw material is blocked, shreds;
(2) add weight ratio 6 ~ 8 times of water gagings, decoct 1h, carry out first time and extract, filter to obtain for the first time filtrate and filtering residue;
(3) filtering residue adds 6 ~ 8 times of water gagings more for the first time, and carry out second time and extract, filter and to obtain second time filtrate and filtering residue, filtering residue discards;
(4) mixing first time filtrate and second time filtrate, being concentrated into relative density is 1.14 ~ 1.18(80 DEG C), deepfreeze;
(5) powdered Exocarpium Citri Grandis extract is namely obtained after carrying out drying.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, described Poria extract is obtained by water boiling and extraction technique.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, the water boiling and extraction technique of described Poria extract comprises the steps:
(1) Poria raw material is blocked, shreds;
(2) add 6 ~ 8 times of water gagings, decoct 1h, carry out first time and extract, filter to obtain for the first time filtrate and filtering residue;
(3) filtering residue adds 6 ~ 8 times of water gagings more for the first time, and carry out second time and extract, filter and to obtain second time filtrate and filtering residue, filtering residue discards;
(4) mixing first time filtrate and second time filtrate, being concentrated into relative density is 1.14 ~ 1.18(80 DEG C), deepfreeze;
(5) powdered Poria extract is namely obtained after carrying out drying.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, described Radix Glycyrrhizae extract is obtained by water boiling and extraction technique.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, the water boiling and extraction technique of described Radix Glycyrrhizae extract comprises the steps:
(1) licorice raw material blocked, shred;
(2) add 6 ~ 8 times of water gagings, decoct 1h, carry out first time and extract, filter to obtain for the first time filtrate and filtering residue;
(3) filtering residue adds 6 ~ 8 times of water gagings more for the first time, and carry out second time and extract, filter and to obtain second time filtrate and filtering residue, filtering residue discards;
(4) mixing first time filtrate and second time filtrate, being concentrated into relative density is 1.14 ~ 1.18(80 DEG C), deepfreeze;
(5) powdered Radix Glycyrrhizae extract is namely obtained after carrying out drying.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, described solubility pharmaceutic adjuvant is auxiliary U.S. powder, glucose, anhydrous glucose, sucrose, shallow lake dimension powder, dextrin, starch, one in sodium bicarbonate or any combination.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, dosage form is peroral dosage form, and usage spice is drunk water.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, dosage form is powder, tablet, granule or oral liquid.
Preferably, the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, described pharmaceutical composition is powder.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) said ratio being taken Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin is raw material;
(2) mix, stir, to obtain final product.
Preferably, the preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) said ratio being taken Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and solubility pharmaceutic adjuvant is raw material;
(2) mix, stir, to obtain final product.
The consumption of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease is for press 0.2g pharmaceutical composition/10g body weight, and oral administration, being used in conjunction 3 is a course for the treatment of.
The invention has the beneficial effects as follows:
The pharmaceutical composition of above-mentioned control livestock and birds respiratory disease; by Chinese medicine and Western medicine reasonable formula; for aquaculture provides a kind of effective medicine preventing and treating livestock and birds respiratory disease; there is cough-relieving, relieving asthma, the effect such as anti-bacteria and anti-virus, lifting immunity of organisms; treating both the principal and secondary aspects of a disease, shortening greatly reduce the occurrence probability of drug resistance while treating the course for the treatment of; its preparation method is simple, easy to use, is applicable to the needs that large-scale industrial is produced.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is further described.
Embodiment 1
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and anhydrous glucose, wherein Exocarpium Citri Grandis extract 10kg, Poria extract 7kg, Radix Glycyrrhizae extract 4kg, fumaric acid tiamulin 11kg, anhydrous glucose 68kg.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) taking above-mentioned Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and anhydrous glucose is raw material;
(2) mix, stir, to obtain final product.
Wherein, described Exocarpium Citri Grandis extract can be obtained by following method:
(1) Exocarpium Citri Grandis raw material is blocked, shreds;
(2) add weight ratio 7 times of water gagings, decoct 1h, carry out first time and extract, filter to obtain for the first time filtrate and filtering residue;
(3) filtering residue adds 7 times of water gagings more for the first time, and carry out second time and extract, filter and to obtain second time filtrate and filtering residue, filtering residue discards;
(4) mixing first time filtrate and second time filtrate, being concentrated into relative density is 1.16(80 DEG C), deepfreeze;
(5) powdered Exocarpium Citri Grandis extract is namely obtained after carrying out drying.
Described Poria extract can be obtained by following method:
(1) Poria raw material is blocked, shreds;
(2) add 7 times of water gagings, decoct 1h, carry out first time and extract, filter to obtain for the first time filtrate and filtering residue;
(3) filtering residue adds 7 times of water gagings more for the first time, and carry out second time and extract, filter and to obtain second time filtrate and filtering residue, filtering residue discards;
(4) mixing first time filtrate and second time filtrate, being concentrated into relative density is 1.16(80 DEG C), deepfreeze;
(5) powdered Poria extract is namely obtained after carrying out drying.
Described Radix Glycyrrhizae extract can be obtained by following method:
(1) licorice raw material blocked, shred;
(2) add 7 times of water gagings, decoct 1h, carry out first time and extract, filter to obtain for the first time filtrate and filtering residue;
(3) filtering residue adds 7 times of water gagings more for the first time, and carry out second time and extract, filter and to obtain second time filtrate and filtering residue, filtering residue discards;
(4) mixing first time filtrate and second time filtrate, being concentrated into relative density is 1.16(80 DEG C), deepfreeze;
(5) powdered Radix Glycyrrhizae extract is namely obtained after carrying out drying.
Embodiment 2
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin, wherein Exocarpium Citri Grandis extract 8kg, Poria extract 2kg, Radix Glycyrrhizae extract 1kg, fumaric acid tiamulin 20kg.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) taking above-mentioned Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin is raw material;
(2) mix, stir, to obtain final product.
Described Exocarpium Citri Grandis extract, Poria extract and Radix Glycyrrhizae extract are all obtained by water boiling and extraction technique.
Embodiment 3
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin, wherein Exocarpium Citri Grandis extract 15kg, Poria extract 10kg, Radix Glycyrrhizae extract 5kg, fumaric acid tiamulin 5kg.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) taking above-mentioned Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin is raw material;
(2) mix, stir, to obtain final product.
Described Exocarpium Citri Grandis extract, Poria extract and Radix Glycyrrhizae extract are all obtained by water boiling and extraction technique.
Embodiment 4
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and auxiliary U.S. powder, wherein Exocarpium Citri Grandis extract 9kg, Poria extract 8kg, Radix Glycyrrhizae extract 3kg, fumaric acid tiamulin 13kg, auxiliary U.S. powder 67kg.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) taking above-mentioned Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and auxiliary U.S. powder is raw material;
(2) mix, stir, to obtain final product.
Described Exocarpium Citri Grandis extract, Poria extract and Radix Glycyrrhizae extract are all obtained by water boiling and extraction technique.
Embodiment 5
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and starch, wherein Exocarpium Citri Grandis extract 12kg, Poria extract 5kg, Radix Glycyrrhizae extract 4kg, fumaric acid tiamulin 8kg, starch 71kg.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) taking above-mentioned Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and starch is raw material;
(2) mix, stir, to obtain final product.
Described Exocarpium Citri Grandis extract, Poria extract and Radix Glycyrrhizae extract are all obtained by water boiling and extraction technique.
Embodiment 6
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin, wherein Exocarpium Citri Grandis extract 10kg, Poria extract 6kg, Radix Glycyrrhizae extract 3.5kg, fumaric acid tiamulin 10kg.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) taking above-mentioned Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and solubility pharmaceutic adjuvant is raw material;
(2) mix, stir, to obtain final product.
Described Exocarpium Citri Grandis extract, Poria extract and Radix Glycyrrhizae extract are all obtained by water boiling and extraction technique.
Embodiment 7
A kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease, by Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin, powder is tieed up in shallow lake and sodium bicarbonate forms, wherein Exocarpium Citri Grandis extract 11kg, Poria extract 6kg, Radix Glycyrrhizae extract 4kg, fumaric acid tiamulin 9kg, dimension of forming sediment powder 65kg, sodium bicarbonate 5kg.
The preparation method of the pharmaceutical composition of above-mentioned control livestock and birds respiratory disease, concrete preparation process is as follows:
(1) taking above-mentioned Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin, shallow lake dimension powder and sodium bicarbonate is raw material;
(2) mix, stir, to obtain final product.
Described Exocarpium Citri Grandis extract, Poria extract and Radix Glycyrrhizae extract are all obtained by water boiling and extraction technique.
The described Chinese crude drug of one of embodiment 1-7 is commercially available prod, and each amounts of components increased according to same ratio or reduce, the parts by weight relation of each component of gained all belongs to protection scope of the present invention.
Chinese crude drug described in embodiment 1-7 all meets " Chinese veterinary pharmacopoeia " version two ministerial standard in 2010.
The composition principle of pharmaceutical composition of the present invention is: in side Exocarpium Citri Grandis extract there is expelling phlegm for arresting cough, relieving dyspepsia of regulating the flow of vital energy, antiinflammatory, the effect such as antioxidation; Poria extract has promoting diuresis to eliminate damp pathogen, strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming, immunostimulant, the effects such as sterilizing and anti-virus antitumor; Radix Glycyrrhizae extract have preventing phlegm from forming and stopping coughing, heat-clearing and toxic substances removing effect, also can coordinating the actions of various ingredients in a prescription; Fumaric acid tiamulin is used for chronic respiratory disease, and mycoplasmal pneumonia of swine and haemophilus pleura pneumonia, also can be used for the dysentery that pig treponema causes.All medicines share, mutual Synergistic, to control livestock and birds respiratory disease remarkably productive, be embodied in following some:
(1) in pharmaceutical composition of the present invention, but Western medicine kills rapidly the cause of disease causing respiratory tract disease, plays the effect effected a permanent cure, Chinese medicine by cough-relieving, relieving asthma, the effect such as lung moistening and assisting sterilisation antiviral, play the effect taken stopgap measures, the two combines, complement each other, treating both the principal and secondary aspects of a disease, improves curative effect;
(2) in side, Poria extract has the effect promoting immunity of organisms, solves immunity of organisms low, is subject to the problem of following pathogen challenge;
(3) by the synergism of Chinese medicine and Western medicine, antibacterial effect is remarkable, and rapidly, Shorten the Treatment Process, decreases the generation of drug resistance in effect;
(4) spice, drinking-water all can use, easy to use, simultaneously because Chinese medicine ingredients adopts extract, solve Chinese medicine content of cellulose high, the heavy problem of poultry.
Clinical trial example 1(mice ammonia draws coughs test):
(1) experimental animal
Get 30 mices, male and female half and half, body weight 20 ± 2g.
(2) trial drug
The solution that concentration is 1g crude drug/mL water is made, 26% ammonia, commercially available Exocarpium Citri Rubrum oral liquid by water-soluble for pharmaceutical composition described in the embodiment of the present invention 1.
(3) EXPERIMENTAL DESIGN and method
30 mices are divided into 3 groups at random, and often organize 10, test group 1 is blank group, by 0.2mL/10g gavage distilled water, and every day 1 time, continuous gavage 3 days; Test group 2 is treatment matched group, by 0.2mL/10g gavage Exocarpium Citri Rubrum oral liquid, and every day 1 time, continuous gavage 3 days; Test group 3 is treatment group, the solution be prepared into by the 0.2mL/10g gavage embodiment of the present invention 1, every day 1 time, continuous gavage 3 days.
After administration terminates 1h, mice is put in glass bell jar and accept 26% ammonia spraying 5s, after spraying terminates, take out mice and observe and the cough latent period recording mice and the cough number of times occurred in 2min.Result of the test is in table 1.
(4) evaluation of result standard and statistical disposition
Mouse cough incubation period: from spray ammonia to produce cough time to mice.
Mouse cough shows: shrink abdominal muscle with mice and dehisce to be as the criterion, sending cough sometimes.
Result is with average ± standard deviation
represent, adopt sas9.0 to carry out data statistic analysis process, two or more sample average compares employing F inspection.
Table 1 mice ammonia draws coughs result of the test
| Group | Test number | Dosage (mL/kg) | Cough latent period (s) | Cough number of times |
| Blank group | 10 | 0 | 25.6±2.81 a | 48.4±6.75 c |
| Treatment matched group | 10 | 0.2 | 32.4±4.86 b | 32.7±6.24 b |
| Treatment group | 10 | 0.2 | 38.3±4.59 b | 26.6±4.42 a |
As seen from the results in Table 1, compare with blank group, the equal significant prolongation of cough latent period (p < 0.01) for the treatment of matched group and treatment group, the cough number of times for the treatment of matched group significantly reduces (p < 0.05), the cough number of times for the treatment of group extremely significantly reduces (p < 0.01), and the cough number of times for the treatment of group comparatively treats the remarkable minimizing of matched group (p < 0.05).
Conclusion: pharmaceutical composition of the present invention has good cough suppressing effect, better compared with the cough suppressing effect of Exocarpium Citri Rubrum oral liquid.
The phenol red expectorant test of clinical trial example 2(mice):
(1) experimental animal
Get 30 mices, male and female half and half, body weight 20 ± 2g.
(2) trial drug
The solution that concentration is 1g crude drug/mL water is made, 2.5% phenol red normal saline solution, commercially available Exocarpium Citri Rubrum oral liquid, the NaOH solution of 1mol/L by water-soluble for pharmaceutical composition described in the embodiment of the present invention 1.
(3) EXPERIMENTAL DESIGN and method
30 mices are divided into 3 groups at random, and often organize 10, test group 1 is blank group, by 0.2mL/10g gavage distilled water, and every day 1 time, continuous gavage 3 days; Test group 2 is treatment matched group, by 0.2mL/10g gavage Exocarpium Citri Rubrum oral liquid, and every day 1 time, continuous gavage 3 days; Test group 3 is treatment group, the solution be prepared into by the 0.2mL/10g gavage embodiment of the present invention 1, every day 1 time, continuous gavage 3 days.
The hungry 16h of mice, after administration terminates 30min, the phenol red normal saline solution 0.1g/10g of lumbar injection 2.5%, puts to death after 30min, is separated trachea from thyroid cartilage to trachea bifurcated section, put into preprepared normal saline, the NaOH solution 2 of instillation 1mol/L, leaves standstill 1h, records A value at 546nm place, last according to phenol red standard curve regression equation, calculate phenol red content.Result of the test is in table 2.
Table 2 mice ammonia draws coughs result of the test
| Group | Test number | Dosage (mL/kg) | The phenol red content of trachea (mg/mL) |
| Blank group | 10 | 0 | 0.13±2.73 c |
| Treatment matched group | 10 | 0.2 | 0.21±3.36 b |
| Treatment group | 10 | 0.2 | 0.29±3.77 a |
As seen from the results in Table 2, compare with blank group, the phenol red content of trachea for the treatment of matched group significantly increases (p < 0.05), the extremely significantly increase (p < 0.01) for the treatment of group.
Conclusion: pharmaceutical composition of the present invention has good expectorant effect, and better compared with the expectorant effect of Exocarpium Citri Rubrum oral liquid.
Above-mentioned detailed description of this kind of pharmaceutical composition preventing and treating livestock and birds respiratory disease and preparation method thereof being carried out with reference to embodiment; illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore in the change do not departed under general plotting of the present invention and amendment, should belong within protection scope of the present invention.
Claims (9)
1. prevent and treat the pharmaceutical composition of livestock and birds respiratory disease for one kind, it is characterized in that: be made up of Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin, described Exocarpium Citri Grandis extract is obtained by water boiling and extraction technique, described Poria extract is obtained by water boiling and extraction technique, described Radix Glycyrrhizae extract is obtained by water boiling and extraction technique, by its weight parts Exocarpium Citri Grandis extract 8 ~ 15 parts, Poria extract 2 ~ 10 parts, Radix Glycyrrhizae extract 1 ~ 5 part, fumaric acid tiamulin 5 ~ 20 parts.
2. the pharmaceutical composition of control livestock and birds respiratory disease according to claim 1, is characterized in that: by its weight parts Exocarpium Citri Grandis extract 9 ~ 12 parts, Poria extract 5 ~ 8 parts, Radix Glycyrrhizae extract 3 ~ 4 parts, fumaric acid tiamulin 8 ~ 13 parts.
3. the pharmaceutical composition of control livestock and birds respiratory disease according to claim 2, is characterized in that: by its weight parts Exocarpium Citri Grandis extract 10 parts, Poria extract 7 parts, Radix Glycyrrhizae extract 4 parts, fumaric acid tiamulin 11 parts.
4. prevent and treat the pharmaceutical composition of livestock and birds respiratory disease for one kind, it is characterized in that: by Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and solubility pharmaceutic adjuvant composition, described Exocarpium Citri Grandis extract is obtained by water boiling and extraction technique, described Poria extract is obtained by water boiling and extraction technique, described Radix Glycyrrhizae extract is obtained by water boiling and extraction technique, wherein the percentage by weight of each component is Exocarpium Citri Grandis extract 8% ~ 15%, Poria extract 2% ~ 10%, Radix Glycyrrhizae extract 1% ~ 5%, fumaric acid tiamulin 5% ~ 20%, solubility pharmaceutic adjuvant polishing to 100%.
5. the pharmaceutical composition of control livestock and birds respiratory disease according to claim 4, it is characterized in that: the percentage by weight of each component is Exocarpium Citri Grandis extract 9 ~ 12%, Poria extract 5 ~ 8%, Radix Glycyrrhizae extract 3 ~ 4%, fumaric acid tiamulin 8 ~ 13%, solubility pharmaceutic adjuvant polishing to 100%.
6. the pharmaceutical composition of control livestock and birds respiratory disease according to claim 5, it is characterized in that: the percentage by weight of each component is Exocarpium Citri Grandis extract 10%, Poria extract 7%, Radix Glycyrrhizae extract 4%, fumaric acid tiamulin 11%, solubility pharmaceutic adjuvant polishing to 100%.
7. the pharmaceutical composition of control livestock and birds respiratory disease according to claim 1, is characterized in that: described pharmaceutical composition is powder.
8. the preparation method of the pharmaceutical composition of the control livestock and birds respiratory disease that one of claim 1-3 is described, concrete preparation process is as follows:
(1) said ratio being taken Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract and fumaric acid tiamulin is raw material;
(2) mix, stir, to obtain final product.
9. the preparation method of the pharmaceutical composition of the control livestock and birds respiratory disease that one of claim 4-6 is described, is characterized in that: concrete preparation process is as follows:
(1) said ratio being taken Exocarpium Citri Grandis extract, Poria extract, Radix Glycyrrhizae extract, fumaric acid tiamulin and solubility pharmaceutic adjuvant is raw material;
(2) mix, stir, to obtain final product.
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| CN102895377B true CN102895377B (en) | 2015-03-11 |
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| CN105030882A (en) * | 2015-07-06 | 2015-11-11 | 四川省畜牧科学研究院 | Medicine compound preparation for preventing and treating respiratory diseases of livestock and poultry, and preparation method thereof |
| CN105902639A (en) * | 2016-06-16 | 2016-08-31 | 福建傲农生物科技集团股份有限公司 | Pharmaceutical preparation for prevention and treatment of chronic respiratory diseases of livestock and poultry and preparation method thereof |
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Inventor after: Hou Jianjun Inventor after: Jiao Weili Inventor after: Wang Jian Inventor after: Cheng Xuejiao Inventor after: Jiao Xiaojun Inventor after: Yu Guiju Inventor after: Wang Yong Inventor after: Wang Meng Inventor after: Gao Rui Inventor before: Jiao Weili Inventor before: Wang Jian Inventor before: Cheng Xuejiao Inventor before: Jiao Xiaojun Inventor before: Yu Guiju Inventor before: Wang Yong Inventor before: Wang Meng Inventor before: Gao Rui |
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Effective date of registration: 20170511 Address after: 166400 Xingshan Industrial Zone, Zhaozhou County, Daqing, Heilongjiang Patentee after: Heilongjiang bone sheep husbandry Co., Ltd. Address before: 300000, Huiyuan Road 16, Zhang Jia nest Industrial Zone, Xiqing District, Tianjin Patentee before: Challenge (Tianjin) Animal Pharmaceutical Co., Ltd. |