CN102894208B - Chinese herbal medicine feedstuff additive used for preventing carassius auratus neosporosis - Google Patents
Chinese herbal medicine feedstuff additive used for preventing carassius auratus neosporosis Download PDFInfo
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- CN102894208B CN102894208B CN2012103072680A CN201210307268A CN102894208B CN 102894208 B CN102894208 B CN 102894208B CN 2012103072680 A CN2012103072680 A CN 2012103072680A CN 201210307268 A CN201210307268 A CN 201210307268A CN 102894208 B CN102894208 B CN 102894208B
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Abstract
The invention discloses a Chinese herbal medicine feedstuff additive used for preventing carassius auratus neosporosis. The Chinese herbal medicine additive is composed of a plurality of Chinese herbal medicines, vitamin C, and wheat middling. With the Chinese herbal medicine additive provided by the invention, carassius auratus neosporosis incidence rate can be substantially reduced, breeding survival rate can be improved, and carassius auratus breeding economy development can be promoted.
Description
Technical field
The present invention relates to the feeding additive aquatic animal field, be specifically related to a kind of for preventing the Chinese herbal feed additive of crucian sporidiosis.
Background technology
Crucian (Carassius auratus) belongs to Cypriniformes, Cyprinidae, crucian carp and belongs to, and is a kind of omnivorefish that plant is food of mainly take, and happiness is trooped and go, selects to eat and occupy.The crucian fine and tender taste, meat flavour is sweet, and nutritive value is very high, and every hectogram meat contains protein 13 grams, fatty 11 grams, and contains the mineral matters such as a large amount of calcium, phosphorus, iron.Crucian is widely distributed, and all there is production in waters in all parts of the country throughout the year, is one of important edible fishes of China.
In recent years, sporozoite occurs serious in the crucian cultivation area, and sporidiosis is rated as the No.1 killer of crucian cultivation.Crucian sporidiosis main harm crucian yearlings and adult fish, often be popular in the annual 4-10 month, and June and September are the peak period of morbidity.Sporozoite mainly attacks the tissues such as the intestines, kidney, liver, brain, muscle of fish, or parasitizes the positions such as the body surface gill, squama, fin, kiss, larynx.The crucian sporidiosis death rate is high, and mere formality is offered report, and it is 1/3 even more that China's North Jiangsu Area crucian sporidiosis death rate usually reaches.
Due to inadequate for sporidiosis research for a long time, about sporozoan epidemiologic feature and the history of life, not yet find out so far, can't take measure targetedly to cut off its propagation and route of transmission, sporidiosis is one of difficult diseases of not yet capturing in China's aquaculture.Sporozoan being encapsulated in ill fish body organ-tissue extensively distributes, and the chitin spore shell of spore outside can resist the invasion and attack of external environment, and medicine can't be penetrated into polypide inside and play a role, and any external used medicine all is difficult to effectively.After sporozoite outburst, because loss is very large, the raiser is impatient disorderly to seek medical advice, and abuses medicine, overdose, during treatment, the externally applied drug improper use, damage water quality, increasing stress, thereby increase the weight of the state of an illness, more the multiple medicines amount is larger for the kind of medication often, dead fish is more serious.
Just because of the particularity of not enough to sporozoite understanding in sporozoite polypide structure adds that the research and comparison to sporidiosis control pharmacopathology lacks, there is no so far the specific medicament for the treatment of sporidiosis.Owing to there is no at present effective therapeutic scheme, the often control for the crucian sporidiosis by part agricultural chemicals veterinary drug of raiser, the phenomenon that medication is not suited the medicine to the illness generally occurs, and agricultural chemicals veterinary drug long-term, high-dose is used, and also causes serious food security hidden danger.
Summary of the invention
Due to sporozoite polypide structure particularity, once crucian suffers from sporidiosis, externally applied drug is difficult to arrive desirable result for the treatment of, harm cultivation caused in order to reduce disease, taking effective precautionary measures particularly important, is approach preferably by adding in feed that Chinese herbal medicine prevents.
The object of the invention is to provide a kind of Chinese herbal composite feed additive that can prevent the crucian sporidiosis.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of prevention crucian sporidiosis Chinese herbal composite feed additive, comprise Chinese herbal medicine (tuber of stemona, purple perilla, pericarpium zanthoxyli, Fructus meliae toosendan, cassia twig, rhizoma zingiberis, kuh-seng, almond, Moschus), vitamin C and inferior powder.
In above-mentioned feed addictive, by percentage to the quality, its component is 12 ~ 18% tubers of stemona, 12 ~ 17% purple perillas, 11 ~ 15% pericarpium zanthoxylis, 8 ~ 10% Fructus meliae toosendans, 5 ~ 8% cassia twig, 5 ~ 8% rhizoma zingiberis, 5 ~ 6% kuh-sengs, 4 ~ 5% almonds, 2 ~ 3% Moschus, 5 ~ 6% vitamin Cs, 16 ~ 19% powder.
A kind of method for preparing above-mentioned prevention crucian sporidiosis Chinese herbal composite feed additive, pulverize described raw material through micronizer, all by 200 purpose screen clothes, after mixing, obtain feed addictive of the present invention.
The using method of above-mentioned prevention crucian sporidiosis Chinese herbal composite feed additive, the percentage by weight according to 0.1 ~ 0.2% adds it in crucian carp feed to, mixes and gets final product.
Compared with prior art, the present invention has following advantage:
(1) Chinese herbal medicine is the rarity in motherland's medical science, as feed addictive, uses and compares with other feed addictives, and it is widely distributed, and toxic and side effect is little, without hormone, is difficult for environment.
The Chinese herbal medicine that the preferred tuber of stemona of the present invention, purple perilla, pericarpium zanthoxyli, Fructus meliae toosendan etc. have insect-expelling function is primary raw material, percentage by weight according to 0.1 ~ 0.2% adds additive of the present invention in crucian carp feed to and can play prevention effect preferably, significantly reduce the sporidiosis incidence of disease, improve the cultivation survival rate, increase economic efficiency.
(2) the present invention is usingd common Chinese herbal medicine as primary raw material, and China's Chinese herbal medicine resource is abundant, and raw material sources are easy.
(3) proportioning of the present invention is simple, operation is simple, with low cost, be suitable for promoting.
The specific embodiment
Further explain the present invention below in conjunction with embodiment, but embodiment does not limit in any form to the present invention.
embodiment 1
Take tuber of stemona 18kg, purple perilla 15kg, pericarpium zanthoxyli 15kg, Fructus meliae toosendan 8kg, cassia twig 8kg, rhizoma zingiberis 5kg, kuh-seng 5kg, almond 5kg, Moschus 2kg, vitamin C 5kg, inferior powder 17kg.Through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in compound crucian feed.
embodiment 2
Take tuber of stemona 15kg, purple perilla 15kg, pericarpium zanthoxyli 14kg, Fructus meliae toosendan 10kg, cassia twig 5kg, rhizoma zingiberis 5kg, kuh-seng 5kg, almond 4kg, Moschus 2kg, vitamin C 6kg, inferior powder 19kg.Through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in compound crucian feed.
embodiment 3
Take tuber of stemona 12kg, purple perilla 17kg, pericarpium zanthoxyli 15kg, Fructus meliae toosendan 8kg, cassia twig 8kg, rhizoma zingiberis 5kg, kuh-seng 6kg, almond 5kg, Moschus 3kg, vitamin C 5kg, inferior powder 16kg.Through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in compound crucian feed.
embodiment 4
Take tuber of stemona 14kg, purple perilla 13kg, pericarpium zanthoxyli 11kg, Fructus meliae toosendan 10kg, cassia twig 8kg, rhizoma zingiberis 8kg, kuh-seng 5kg, almond 5kg, Moschus 3kg, vitamin C 5kg, inferior powder 18kg.Through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in compound crucian feed.
embodiment 5
Take tuber of stemona 18kg, purple perilla 12kg, pericarpium zanthoxyli 15kg, Fructus meliae toosendan 8kg, cassia twig 8kg, rhizoma zingiberis 5kg, kuh-seng 5kg, almond 5kg, Moschus 2kg, vitamin C 5kg, inferior powder 17kg.Through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in compound crucian feed.
the concrete application case of embodiment 6
Chinese herbal feed additive of the present invention not only can effectively prevent the crucian sporidiosis to occur, and improves the cultivation survival rate, can also reduce feed coefficient, for the raiser brings good economic benefit simultaneously.Below in conjunction with concrete application case, be illustrated.
1 materials and methods
1.1 experimental design
Single factor experiment design, totally 3 processed group, 3 repetitions of each processed group.Each processed group all adopts identical basic components, A processed group (basic components blank), B processed group (additive that basic components+0.2% patent working example 1 of the present invention is produced), C processed group (additive that basic components+0.2% patent working example 2 of the present invention is produced).
1.2 test fish
Carassius auratus gibelio species 900 tails by initial body weight 20g left and right, be divided into 3 groups at random, every group of 300 tails, and each repeats 100 tails.Before formal test starts, carry out trial test and support 1 week temporarily.
1.3 test method and management
1.3.1 experimental condition
Carry out in the net cage that test is set up on 13 mu of hydrostatic pools of a bite, within every 2 weeks, change 1/3 fresh water, average water temperature 22-24 ℃.The test before with quick lime, Chi Shui is carried out disinfection, during the fingerling cartonning with the processing that disinfects with salt solution.
1.3.2 daily management
Every morning 9:00 and afternoon 15:00 throw something and feed twice, be satiated with food and throw something and feed, with material platform after throwing something and feeding 30 minutes, without residual bait residue, be as the criterion, feeding rate, at 2-4%, is adjusted weekly feeding volume one time according to weight gain and residual bait residue situation.Record food ration, water temperature, temperature every day, record every group of dead quantity and weight of test fish, death test fish is visually observed with microscopy and causes death to determine whether sporidiosis, record the dead test of sporidiosis fish quantity.Test while within 8 weeks, finishing and weigh on an empty stomach.
1.3.2 test index
Be calculated to be motility rate, the sporidiosis death rate, feed coefficient after testing end in 8 weeks.
1.4 data are processed
Carry out one-way analysis of variance with SPSS18.0 software, and carry out multiple ratio, data result means with means standard deviation.
2 results and analysis
2.1 the impact of Chinese herbal feed additive on the sporidiosis death rate
The impact of table 1 Chinese herbal feed additive on the crucian death rate
| Group | Initial mantissa's (tail) | Whole end number (tail) | The sporidiosis death rate (%) |
| A | 100.00±0.00 | 82.33±4.04A | 65.72±2.14B |
| B | 100.00±0.00 | 94.67±1.53B | 11.43±10.30A |
| C | 100.00±0.00 | 94.00±2.00B | 9.72±8.67A |
Annotate: adopt the Duncan method relatively, the different letter representation significant differences (P<0.05) such as same row small letter abcd, the different letter representation differences such as same row capitalization ABCD are (P<0.01) extremely significantly.
After test in 8 weeks, do not add the whole end of the control group number of Chinese herbal feed additive extremely significantly lower than two groups that add prevention crucian sporidiosis Chinese herb feed additive, the sporidiosis death rate of the control group also utmost point is significantly higher than other two groups.Chinese herbal feed additive can extremely significantly reduce the crucian sporidiosis death rate.
2.1 the impact of Chinese herbal feed additive on the crucian growth
The impact of table 2 Chinese herbal feed additive on the crucian growth
| Group | Initial weight (kg) | Initial mantissa's (tail) | Whole end number (tail) | Whole last weight (kg) | Feed coefficient |
| A | 1.95±0.01 | 100.00±0.00 | 82.33±4.04A | 2.06±0.08A | 2.64±0.08B |
| B | 1.94±0.02 | 100.00±0.00 | 94.67±1.53B | 2.48±0.11B | 2.16±0.04A |
| C | 1.94±0.02 | 100.00±0.00 | 94.00±2.00B | 2.59±0.09B | 2.06±0.08A |
Annotate: adopt the Duncan method relatively, the different letter representation significant differences (P<0.05) such as same row small letter abcd, the different letter representation differences such as same row capitalization ABCD are (P<0.01) extremely significantly.
As can be seen from Table 2, after test in 8 weeks, Chinese herbal feed additive can extremely significantly reduce feed coefficient.To sum up, Chinese herbal feed additive can significantly improve the cultivation survival rate, reduces the crucian sporidiosis death rate, can reduce feed coefficient simultaneously.
Claims (3)
1. one kind for preventing the Chinese herbal feed additive of crucian sporidiosis, it is characterized in that being formed by the following component according to the mass percent meter: 12 ~ 18% tubers of stemona, 12 ~ 17% purple perillas, 11 ~ 15% pericarpium zanthoxylis, 8 ~ 10% Fructus meliae toosendans, 5 ~ 8% cassia twig, 5 ~ 8% rhizoma zingiberis, 5 ~ 6% kuh-sengs, 4 ~ 5% almonds, 2 ~ 3% Moschus, 5 ~ 6% vitamin Cs, 16 ~ 19% powder.
2. the described preparation method for the Chinese herbal feed additive that prevents the crucian sporidiosis of claim 1, is characterized in that each component is pulverized through micronizer, all by 200 eye mesh screens, after mixing, obtains.
3. the described using method for the Chinese herbal feed additive that prevents the crucian sporidiosis of claim 1, is characterized in that according to the ratio of 0.1 ~ 0.2 quality %, additive being added in crucian carp feed, mixes and get final product.
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| CN105249025A (en) * | 2015-11-13 | 2016-01-20 | 广西壮族自治区中国科学院广西植物研究所 | Application of sophora flavescens extract or sophora flavescens ultra-fine powder in preparing fodder additives |
| CN106417991A (en) * | 2016-08-22 | 2017-02-22 | 安徽麒麟正大饲料有限公司 | Feed for preventing myxosporidiosis of crucian carps and preparation method of feed |
| CN106306365A (en) * | 2016-08-23 | 2017-01-11 | 合肥申仁养殖有限公司 | Selenium-rich black carp feed |
| CN108721417A (en) * | 2018-04-19 | 2018-11-02 | 广东联鲲集团有限公司 | A kind of additive and its preparation method and application for preventing hito enteritis |
| CN111034657A (en) * | 2019-12-10 | 2020-04-21 | 浙江省淡水水产研究所 | Method for marking rough micropterus salmoides fries by using circulating water culture tank in pond |
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| CN101040936B (en) * | 2007-04-28 | 2011-10-19 | 大连水产学院 | Compound Chinese traditional medicine agent for preventing fish verminosis |
| CN101156940B (en) * | 2007-09-12 | 2010-06-02 | 严必福 | Sporozoan cleaning fish medicine |
| CN101912591A (en) * | 2010-07-29 | 2010-12-15 | 重庆市南川区金佛山中药材品种培育研究所 | Chinese medicinal herb composition for curing biliary tract ascariasis |
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Address after: 511422, No. 18, No. six, North Main Street, South Village, Panyu District, Guangzhou, Guangdong Patentee after: Guangdong Haid Group Co., Ltd. Patentee after: GUANGZHOU HINTER BIOTECHNOLOGY CO., LTD. Address before: 511422, No. 18, No. six, North Main Street, South Village, Panyu District, Guangzhou, Guangdong Patentee before: Guangdong Haid Group Co., Ltd. Patentee before: Guangzhou Hinter Bio-technology Co., Ltd. |