CN102875204A - Prescription and technology for developing growth promoting organic fertilizer through algal mud and rotten chicken manure - Google Patents
Prescription and technology for developing growth promoting organic fertilizer through algal mud and rotten chicken manure Download PDFInfo
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- CN102875204A CN102875204A CN2012104360793A CN201210436079A CN102875204A CN 102875204 A CN102875204 A CN 102875204A CN 2012104360793 A CN2012104360793 A CN 2012104360793A CN 201210436079 A CN201210436079 A CN 201210436079A CN 102875204 A CN102875204 A CN 102875204A
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Abstract
The invention provides a technology for growth promoting organic fertilizer through algal mud and rotten chicken manure and a product of the technology, belonging to the agricultural intensive production technology. The prescription includes common rotten chicken mature (93% based on weight ratio, similarly hereinafter) and algal mud salved from Lake Taihu (7% of dry matter); the technical process comprises the following steps: mixing the raw materials based on the optimal prescription, fermenting in strip stack type, and overturning once each day, and fermenting for 6 days. The prepared fertilizer includes 2 to 3% of total nitrogen, 5 to 7% of total azophoska, over 45% organic matter, and less than 4 mu g/kg microcystic toxins LR and RR; and the fertilizer is rich in beneficial amino acid; and the free amino acid content is 8.4g/kg. The growth promoting organic fertilizer provided by the invention has obvious growth promoting effect to the industrial crops and the food crops.
Description
One, technical field
The invention provides a kind of algae mud and short technique of giving birth to fertilizer of Chicken dung development and products thereof utilized, belong to the intensive agriculture production technology, this short fertilizer of giving birth to is rich in a large amount of amino acid, can promote the growth of crop.
Two, background technology
Along with the constant growth of productivity, improving constantly of people's living standard, a large amount of sanitary sewages and industrial and agricultural wastewater and waste enter in river, lake and the reservoir, and the nutritive substance that enters water body constantly increases, and cause the eutrophication of water body in lake to be on the rise.Body eutrophication can cause the explosive breeding of blue-green algae big area.The amount reproduction of blue-green algae not only can suppress the growth of other algae, the transparency of water body is descended, also can discharge poisonous metabolic substd to water body, and after a large amount of blue-green algae death, can cause the dissolved oxygen wretched insufficiency of water body, produce stench, consisted of serious threat for ecotope and human health.The method of removing blue-green algae is a lot, directly removes blue-green algae in the water body, and shifts out water body, namely salvages the blue-green algae method, and this is that very effective and academia does not have controversial a kind of method at present.Behind the blue algae bloom of Taihu Lake, directly salvage blue-green algae as emergency schedule, have every day more than 1000 ton of blue algae water from the water body of Taihu Lake, to salvage, approximately 180,000 tons of accumulative totals.Processing after the salvaging of blue-green algae and the salvaging is a large problem demanding prompt solution in the present stage Taihu Lake governance process.But blue-green algae has obvious detrimentally affect to domestic animal, poultry growth, causes the sex change of animal liver, kidney, should not directly utilize.In today of energy shortage, environmental pollution, discarded biomass energy is converted into the available chemical energy source of people, more and more become the focus that people pay close attention to.So both can solve problem of environmental pollution, again can alleviating energy crisis.Blue-green algae mainly contains microcystic aeruginosa, Anabaena Flos-aquae and variation anabena etc., above 3 kinds of blue-green algaes contain abundant nutritive ingredient, there is the expert that the amino acid of these several algaes and the content of lipid acid are measured, and contrasts with other algae (green alga, hidden algae, Euglena and diatom).The aminoacids content of finding three kinds of blue-green algaes is all higher than other algae, wherein all the highest with leucine content in Microcystis aeruginosa and the anabena.Contain the abundant nutrition compositions such as vegetable-protein, polysaccharide in the blue-green alga bloom, therefore imperative to the wasted resources of salvaging algae mud in recent years.Salvage the water content of algae mud between 86-89%, the full N content of algae mud dry-matter is 7.5-8.5%, and full P content is 0.44%, and full K content is 0.5%; Organic content is greater than 70%.(1, Shen Yinwu, Liu Yongding, Wu Guoqiao, etc. the mechanical removal [J] of eutrophic lake Dian Chi bloom blue algae. the hydrobiont journal, 2004,25 (2): research Southern Yangtze University Master's thesis 2009 page 2 of 131-136.2, the quick aerobic compost of blue-green algae).But raw material blue-green algae the inside contains the macromolecular substance such as toxin, albumen, without processing meeting secondary pollution environment (nitrogen, phosphorus, algae toxin), and nutrient can not be directly by plants utilization (Du Jing, " research of Taihu Lake bloom blue algae resource technology " [D], Agricultural University Of Nanjing, 2008, master thesis; Han Zhiguo, Wu Baowang, Zheng Xiesheng, etc. the cyanophycean toxin progress (summary) [J] in the poisons in freshwater. Ji'nan University's journal: natural science edition, 2001,22 (3): 129-134; Yang Hailin, Li Kelang, Zhang Ling, etc. the research [J] of blue algae resource innoxious use technology. biotechnology, 2008,18 (6): 95-98).This patent is salvaged the amino acid that enriches that algae mud is rich in from Taihu Lake, utilize such protein resource and common Chicken dung compost secondary solid fermentation banana to urge living microorganism, has developed short living fertilizer.
Contain a large amount of amino acid whose fertilizers and can promote plant growth.Can produce in the various active substance dissolves soil insoluble compound behind such organic fertilizer application and absorb nutrient to increase soil fertility and to be conducive to farm crop.The key that such fertilizer plays a role is amino acid whose content in the fertilizer, and the aminoacids content that therefore studying different prescriptions increases in the fertilizer becomes particularly important.The kind of exogenous amino acid is a lot, and such as dish cake, soya-bean cake etc., but this amino acid cost is very high, and especially soya-bean cake is sought with the low-cost amino acid of development of new and then can be increased substantially the short market competitiveness of giving birth to fertilizer.
Three, summary of the invention
Technical problem the object of the invention is to salvage algae mud resource for Taihu Lake can't take full advantage of problem with amino acid fertilizer production cost costliness, develop a kind of novel short prescription and technique of giving birth to fertilizer of amino acid that is rich in, reduce the production cost of fertilizer, the short living fertilizer of production can significantly promote the growth of crop.
Be main contents of the present invention below the technical scheme: a kind of algae mud and short technique of giving birth to fertilizer of Chicken dung development utilized comprises:
Chicken dung is fertile: algae mud mixes than 9.3: 0.7 by dry weight; Chicken dung is fertile and algae mud mixture carries out solid fermentation again, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, ferment and finished afterwards in 6 days, the water content that is no more than under 60 ℃ the condition fertilizer in temperature at last is evaporated to below 30%, and packing is dispatched from the factory and is the short organic fertilizer of giving birth to of finished product.Its germination index of Chicken Manure Compost after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=45%, and the organonitrogen quality is 2-3% than content, the water content mass ratio is 25-30%.
Short living organic fertilizer with above-mentioned Recipe production, total nitrogen content is 2-3%, total nitrogen phosphorus potassium nutrition is 5-7%, organic content 〉=45%, free aminoacid content 8.4g/kg (fertilizer dry weight), and the content of microcysin LR and RR all is lower than 4ug/kg in this product.
Beneficial effect the invention provides a kind of prescription and technique of utilizing the development of algae mud to be rich in the short living fertilizer of amino acid.
It is low, easy to use that the short living fertilizer that uses this invention to produce has a production cost, shortly gives birth to effective advantage, is fit to cash crop and food crop producing region big area and uses.The present invention is for preserving the ecological environment, and the protection people's is healthy, and the added value that improves agricultural-food has great importance.
Product is compared with product in the market has following advantage:
1) this patent utilizes Taihu Lake to salvage algae mud and the common fertile conduct prescription of Chicken dung, the short fertilizer of giving birth to of solid fermentation development, this prescription and technique can increase considerably amino acid whose content in such fertilizer, substantially do not increase the cost of fertilizer, the simultaneously effect by microorganism, the algae toxin can be greatly reduced, and the product of production has the extremely strong market competitiveness.Product utilization algae mud of the present invention is solid fermentation raw materials, can reduce the raw materials cost of amino acid fertilizer.Protein content can be up to more than 50% in the algae mud, and amino acid profiles is more complete.
2) content of algae toxin LR and RR (microcystin-LR and microcystin-RR) in the employing liquid chromatography for measuring biological organic fertilizer, the result shows that the content of microcysin LR and RR all is lower than 4ug/kg (fertilizer dry weight), the content of microcysin LR and RR is respectively 3.1mg/kg (algae mud fresh weight) and 62.16mg/kg (algae mud fresh weight) in the pure algae mud of raw material and we record, therefore can be with the content of algae toxin LR and RR below 4ug/kg (fertilizer dry weight) behind the algae mud recycling the art of this patent solid fermentation the bimester of the placement, the standard that does not also have at present algae toxin in the fertilizer both at home and abroad, standard in the underground water is only arranged, be lower than 1ug/kg.
3) contain abundant dissolved organic carbon nitrogen and the total free aminoacids of A wide selection of colours and designs in the fertilizer product, can provide very superior growing environment to crop.Test-results shows, after cash crop and food crop are used this product on seedling stage, can significantly promote plant growth.
Four, description of drawings
Amino acid whose content in the short living fertilizer that Fig. 1 different content algae mud is developed into
The short pot test effect figure that gives birth to of Fig. 2 banana
The short pot test effect figure that gives birth to of Fig. 3 cucumber
The short pot test effect figure that gives birth to of Fig. 4 corn
Five, embodiment
1, the salvaging of Taihu Lake algae mud
Salvage the station by the Taihu Lake periphery and provide free, placed algae mud storage pool 2 months.
2, solid fermentation generates the short fertilizer of giving birth to
2.1 the acquisition of solid fermentation prescription
With algae mud according to different gradients with become thoroughly decomposed after chicken manure carry out mixed stack, the heap body be 5 kilograms (dry weights).About 6 days, the variation of temperature in the monitoring composting process, the sample of getting 5 days is measured aminoacids content (Fig. 1), the result shows, except the interpolation combination of 9% content, temperature presents along with the blue-green algae amount increases and the trend of rising, and temperature tends towards stability in the time of 6 days, infer former because 9% addition water content is too high, the compost failure.Fig. 1 shows, 7% blue-green algae (dry weight) addition aminoacids content is the highest.Its germination index of Chicken Manure Compost after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=45%, and the organonitrogen quality is 2-3% than content, the water content mass ratio is 25-30%.
2.2 the fermentation of solid raft generates the short fertilizer of giving birth to
With algae mud according to 7% addition (dry weight) with become thoroughly decomposed after chicken manure carry out mixed stack, the heap body is 100 kilograms (dry weights).About 6 days, the variation of temperature in the monitoring composting process, it is 8.4g/kg (fertilizer dry weight) that the sample of getting 6 days is measured aminoacids content.Carry out the detection of residual algae toxin, method is as follows again: add the acetic acid of 100mL5% in the 100g fertilizer ,-70 and normal temperature under multigelation repeatedly after, the centrifugal 10min of 12000r/min takes out respectively supernatant liquor (I) and precipitation; To precipitate with the concussion of 100mL80% methanol solution and extract 4h, centrifugal 15min gets supernatant liquor (II); With supernatant liquor (I) with after supernatant liquor (II) fully mixes supernatant liquor (III), supernatant liquor (III) is transferred to 3 with dilute hydrochloric acid with pH, centrifugal after the standing over night, abandon residue, get supernatant liquor (IV), after supernatant liquor (IV) transferred to 7 with weak ammonia with pH, 40 ℃ of rotary evaporations were removed methyl alcohol, remaining sample is crossed 0.45 μ m filter membrane get algae toxin crude extract.Use successively 20mL100% methyl alcohol, 20mL ultrapure water activation C18 solid-phase extraction column (500mg/6ml), algae toxin crude extract is carried out enrichment by solid-phase extraction column concentrated, use respectively again 20mL10% and 20ml20% methanol solution drip washing stationary phase, the Microcystin of using at last 10mL80% methanol solution (adding 0.05% trifluoroacetic acid) will be adsorbed on the stationary phase elutes, Microcystin behind the purifying is removed methyl alcohol by rotary evaporation get the MCs storing solution, after the storing solution lyophilize, to 2ml, liquid is for the upper machine testing of HPLC with 20% methanol constant volume.
The content of algae toxin LR and RR (microcystin-LR and microcystin-RR) in the employing liquid chromatography for measuring biological organic fertilizer, the result shows that the content of microcysin LR and RR all is lower than 4ug/kg (fertilizer dry weight), the content of microcysin LR and RR is respectively 3.1mg/kg (algae mud fresh weight) and 62.16mg/kg (algae mud fresh weight) in the pure algae mud of raw material and we record, therefore can be with the content of algae toxin LR and RR below 4ug/kg (fertilizer dry weight) behind the algae mud recycling the art of this patent solid fermentation the bimester of the placement, the standard that does not also have at present algae toxin in the fertilizer both at home and abroad, standard in the underground water is only arranged, be lower than 1ug/kg.
3, the short fruit test that comes into force in greenhouse
3.1 the short effect of fertilizer on banana of giving birth to
Short living test is carried out in workstation greenhouse, Agricultural University Of Nanjing Hainan, establishes altogether processing of a contrast, processes and adds the short living fertilizer (AOF) that this patent is developed, and contrasts to be common chicken manure organic fertilizer (OF).Each processes 30 basins, every basin dress 10kg soil, and the addition of fertilizer is 2%.Fertilizer and soil fully mix, Routine Management.Results of regular determination Banana Growth index.Result such as table one, table two, table three, table four, table five, table six, table seven and table eight, short give birth to plant height, stem that using of fertilizer can significantly increase banana plant thick, fall three leaf areas, chlorophyll content, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weights.The green cellulose content of disleaf, stem is thick and plant height outside, other indexs namely arrived significant difference at the 10th day.
The different fertilizers of table one are on the unit that affects of banana plant height: cm
The different fertilizers of table two on the banana stem thick affect unit: mm
The different fertilizers of table three affect unit: cm to what banana fell three leaf leaf areas
2
The different fertilizers of table four are on the impact of banana plant chlorophyll content
The different fertilizers of table five affect unit: g to banana overground part fresh weight
The different fertilizers of table six affect unit: g to banana overground part dry weight
The different fertilizers of table seven affect unit: g to banana underground part fresh weight
The different fertilizers of table eight affect unit: g to banana underground part dry weight
3.2 the short effect of fertilizer on cucumber of giving birth to
Establish altogether processing of a contrast, the short living fertilizer (AOF) of this patent development is added in processing, contrasts to be common chicken manure organic fertilizer (OF).Each processes 30 basins, every basin dress 10kg soil, and the addition of fertilizer is 2%.Fertilizer and soil fully mix, Routine Management.Results of regular determination cucumber growth index.Result such as table one, table two, table three, table four, table five, table six, table seven and table eight, short give birth to plant height, stem that using of fertilizer can significantly increase cucumber plant thick, fall three leaf areas, chlorophyll content, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weights.Plant height reaches significant difference after transplanting seedlings 20 days, the thick and leaf area of stem reaches significant difference after transplanting seedlings 40 days, and chlorophyll content reached significant difference after 30 days.
The different fertilizers of table one are on the unit that affects of cucumber plant height: cm
The different fertilizers of table two on Cucumis sativus stem thick affect unit: mm
The different fertilizers of table three affect unit: cm to cucumber accumulation leaf area
2
The different fertilizers of table four are on the impact of three leaf chlorophyll contents of cucumber
The different fertilizers of table five affect unit: g to cucumber overground part fresh weight
The different fertilizers of table six affect unit: g to cucumber overground part dry weight
The different fertilizers of table seven affect unit: g to cucumber underground part fresh weight
The different fertilizers of table eight affect unit: g to cucumber underground part dry weight
3.3 the short effect of fertilizer on corn of giving birth to
Establish altogether processing of a contrast, the short living fertilizer (AOF) of this patent development is added in processing, contrasts to be common chicken manure organic fertilizer (OF).Each processes 30 basins, every basin dress 10kg soil, and the addition of fertilizer is 2%.Fertilizer and soil fully mix, Routine Management.The growth indexes of results of regular determination corn.Result such as table one, table two, table three, table four, table five, table six, table seven and table eight, short give birth to plant height, stem that using of fertilizer can significantly increase milpa thick, fall three leaf areas, chlorophyll content, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weights.All indexs namely reached significant difference at the 10th day.
The different fertilizers of table one are on the unit that affects of corn plant height: cm
The different fertilizers of table two on Maize Stem thick affect unit: mm
The different fertilizers of table three affect unit: cm to what corn fell three leaf leaf areas
2
The different fertilizers of table four are on the impact of three leaf chlorophyll contents of corn
The different fertilizers of table five affect unit: g to corn field top fresh weight
The different fertilizers of table six affect unit: g to corn field top dry weight
The different fertilizers of table seven affect unit: g to corn field bottom fresh weight
The different fertilizers of table eight affect unit: g to corn field bottom fresh weight
Claims (4)
1. one kind is utilized algae mud and the short technique of giving birth to fertilizer of Chicken dung development, comprising:
1) Chicken dung is fertile: algae mud mixes in 9.3: 0.7 ratios of dry weight;
2) Chicken dung is fertile and algae mud mixture carries out solid fermentation, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, ferment and finished afterwards in 6 days, the water content that is no more than under 60 ℃ the condition fertilizer in temperature at last is evaporated to below 30%, and packing is dispatched from the factory and is the short organic fertilizer of giving birth to of finished product;
Its germination index of Chicken Manure Compost after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=45%, and the organonitrogen quality is 2-3% than content, the water content mass ratio is 25-30%.
2. the short living organic fertilizer product of the described explained hereafter of claim 1, the content of microcysin LR and RR all is lower than 4ug/kg in this product.
3. short living organic fertilizer product according to claim 1, total nitrogen content is 2-3% in this product, total nitrogen phosphorus potassium nutrition is 5-7%, organic content 〉=45%, free aminoacid content 8.4g/kg fertilizer dry weight.
4. the application of claim 2 and 3 described short living organic fertilizer products.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104030855A (en) * | 2014-07-07 | 2014-09-10 | 江苏天成科技集团有限公司 | Production process for relay fermentation of novel plant growth promoting rhizobacteria type bio-organic fertilizer |
| CN104119123A (en) * | 2014-08-18 | 2014-10-29 | 广西宁明百事康生物工程有限公司 | Method for preparing fertilizer special for sugar cane |
| CN104261981A (en) * | 2014-09-19 | 2015-01-07 | 南京农业大学 | Preparation method of seaweed organic fertilizer and organic fertilizer prepared by using method |
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| CN102627499A (en) * | 2012-04-12 | 2012-08-08 | 中国农业大学烟台研究院 | Biological organic fertilizer special for cucumber |
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| CN101255075A (en) * | 2008-03-20 | 2008-09-03 | 江南大学 | Improved blue algae treatment utilization method |
| CN102627499A (en) * | 2012-04-12 | 2012-08-08 | 中国农业大学烟台研究院 | Biological organic fertilizer special for cucumber |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104030855A (en) * | 2014-07-07 | 2014-09-10 | 江苏天成科技集团有限公司 | Production process for relay fermentation of novel plant growth promoting rhizobacteria type bio-organic fertilizer |
| CN104119123A (en) * | 2014-08-18 | 2014-10-29 | 广西宁明百事康生物工程有限公司 | Method for preparing fertilizer special for sugar cane |
| CN104119123B (en) * | 2014-08-18 | 2016-01-20 | 广西宁明百事康生物工程有限公司 | A kind of preparation method of sugarcane special-purpose fertilizer |
| CN104261981A (en) * | 2014-09-19 | 2015-01-07 | 南京农业大学 | Preparation method of seaweed organic fertilizer and organic fertilizer prepared by using method |
| CN104261981B (en) * | 2014-09-19 | 2017-06-16 | 南京农业大学 | The preparation method of seaweed organic fertilizer material and the organic fertilizer being made of the method |
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