Summary of the invention
Goal of the invention: the object of the invention is to for the deficiencies in the prior art, a kind of health food of high body immunity is provided.
Another object of the present invention is to provide the preparation method of above-mentioned health food.
Technical scheme: in order to achieve the above object, the present invention specifically is achieved like this: a kind of health food that improves body immunity comprises the component of following percentage by weight: 5 ~ 15% Chinese caterpillar fungus, 5 ~ 15% sea-buckthorn, 5 ~ 20% matrimony vine, 10 ~ 30% sealwort, 10 ~ 30% radix polygonati officinalis and 20 ~ 50% honey.
Wherein, described Chinese caterpillar fungus is natural cs.
Wherein, described sea-buckthorn is fresh syrup of Fructrs Hippophae.
Prepare the method for the health food of above-mentioned raising body immunity, may further comprise the steps:
(1) measure Chinese caterpillar fungus by prescription, extract 3 times with alcohol reflux, dregs of a decoction extracting in water secondary merges the extract Recycled ethanol, is concentrated into relative density 1.30 under 20 ℃, and is for subsequent use;
(2) measure sealwort, radix polygonati officinalis, matrimony vine by prescription and extract secondary with poach, extract and fresh syrup of Fructrs Hippophae also are concentrated into relative density 1.30 together under 20 ℃, for subsequent use;
(3) step (1) alcohol extracting cream and step (2) water extraction cream are merged, add ethanol precipitation secondary, refrigerate 5 days for the first time, refrigerate 3 days for the second time, get the supernatant Recycled ethanol after, for subsequent use;
(4) measure the honey thin up by prescription after, be heated to and dissolve, with diatomite filtration to the clarification, for subsequent use;
(5) combining step (3) gained liquid adds step (4) gained honey, and the distilled water that adds again 5 times of raw material gross weights is mixed well, and transfers pH to 9.7 ~ 10.0 with NaOH solution, filters;
(6) with step (5) gained filled with solution, every 10ml, after the sealing 120 ℃ of lower pressure sterilizings 35 minutes, both.
Wherein, in the step (1), Chinese caterpillar fungus is that to extract 4 hours, its 8 times of quality purities be that to extract 3 hours, its 6 times of quality purities be that 75% alcohol reflux extracted 2 hours for 80% alcohol reflux for 85% alcohol reflux with its 8 times of quality purities respectively.
Wherein, in the step (1), the dregs of a decoction are got 3 hours, the water extraction of its 8 times of quality with the water extraction of its 10 times of quality respectively and were got 2 hours.
Wherein, in the step (2), sealwort, radix polygonati officinalis and matrimony vine were got 2 hours with the water extraction that the water extraction of its quality and 10 times is got 3 hours, its quality and 8 times respectively.
Wherein, in the step (3), be respectively 70%, 75% ethanol precipitation with purity.
Beneficial effect: it is raw material that the present invention selects natural medicine, food, processes with modern preparation process, does not bring other chemical constituents in preparation process into, can reach to strengthen the body immunity; Simultaneously, the present invention also has good action to regulating blood fat.
The specific embodiment
Embodiment 1:
Health food consumption (unit is mass percent): 5% Chinese caterpillar fungus, 10% matrimony vine, 30% sealwort, 30% radix polygonati officinalis, 5% sea-buckthorn and 20% honey.
The preparation method: get Chinese caterpillar fungus, extract 3 times with alcohol reflux, dregs of a decoction extracting in water secondary merges the extract Recycled ethanol, is concentrated into relative density 1.30 under 20 ℃, and is for subsequent use; Get sealwort, radix polygonati officinalis, matrimony vine and extract secondary with poach, extract and fresh syrup of Fructrs Hippophae also are concentrated into relative density 1.30 together under 20 ℃, for subsequent use; Above-mentioned alcohol extracting cream and water extraction cream are merged, add ethanol precipitation secondary, refrigerate 5 days for the first time, refrigerate 3 days for the second time, get the supernatant Recycled ethanol after, for subsequent use; After getting the honey thin up, be heated to and dissolve, to clarification, for subsequent use with diatomite filtration; Merge gained liquid after the above-mentioned refrigeration, add honey for subsequent use, the distilled water that adds again 5 times of raw material gross weights is mixed well, and transfers pH to 9.7 to filter with NaOH solution; With the gained filled with solution, every 10ml, after the sealing 120 ℃ of lower pressure sterilizings 35 minutes, both.
Embodiment 2:
Health food consumption (unit is mass percent): 5% Chinese caterpillar fungus, 20% matrimony vine, 15% sealwort, 20% radix polygonati officinalis, 10% sea-buckthorn and 30% honey.
The preparation method: get Chinese caterpillar fungus, extract 3 times with alcohol reflux, dregs of a decoction extracting in water secondary merges the extract Recycled ethanol, is concentrated into relative density 1.30 under 20 ℃, and is for subsequent use; Get sealwort, radix polygonati officinalis, matrimony vine and extract secondary with poach, extract and fresh syrup of Fructrs Hippophae also are concentrated into relative density 1.30 together under 20 ℃, for subsequent use; Above-mentioned alcohol extracting cream and water extraction cream are merged, add ethanol precipitation secondary, refrigerate 5 days for the first time, refrigerate 3 days for the second time, get the supernatant Recycled ethanol after, for subsequent use; After getting the honey thin up, be heated to and dissolve, to clarification, for subsequent use with diatomite filtration; Merge gained liquid after the above-mentioned refrigeration, add honey for subsequent use, the distilled water that adds again 5 times of raw material gross weights is mixed well, and transfers pH to 9.8 to filter with NaOH solution; With the gained filled with solution, every 10ml, after the sealing 120 ℃ of lower pressure sterilizings 35 minutes, both.
Embodiment 3:
Health food consumption (unit is mass percent): 5% Chinese caterpillar fungus, 5% matrimony vine, 20% sealwort, 15% radix polygonati officinalis, 15% sea-buckthorn and 40% honey.
The preparation method: get Chinese caterpillar fungus, extract 3 times with alcohol reflux, dregs of a decoction extracting in water secondary merges the extract Recycled ethanol, is concentrated into relative density 1.30 under 20 ℃, and is for subsequent use; Get sealwort, radix polygonati officinalis, matrimony vine and extract secondary with poach, extract and fresh syrup of Fructrs Hippophae also are concentrated into relative density 1.30 together under 20 ℃, for subsequent use; Above-mentioned alcohol extracting cream and water extraction cream are merged, add ethanol precipitation secondary, refrigerate 5 days for the first time, refrigerate 3 days for the second time, get the supernatant Recycled ethanol after, for subsequent use; After getting the honey thin up, be heated to and dissolve, to clarification, for subsequent use with diatomite filtration; Merge gained liquid after the above-mentioned refrigeration, add honey for subsequent use, the distilled water that adds again 5 times of raw material gross weights is mixed well, and transfers pH to 9.9 to filter with NaOH solution; With the gained filled with solution, every 10ml, after the sealing 120 ℃ of lower pressure sterilizings 35 minutes, both.
Embodiment 4:
Health food consumption (unit is mass percent): 15% Chinese caterpillar fungus, 10% matrimony vine, 10% sealwort, 10% radix polygonati officinalis, 5% sea-buckthorn and 50% honey.
The preparation method: get Chinese caterpillar fungus, extract 3 times with alcohol reflux, dregs of a decoction extracting in water secondary merges the extract Recycled ethanol, is concentrated into relative density 1.30 under 20 ℃, and is for subsequent use; Get sealwort, radix polygonati officinalis, matrimony vine and extract secondary with poach, extract and fresh syrup of Fructrs Hippophae also are concentrated into relative density 1.30 together under 20 ℃, for subsequent use; Above-mentioned alcohol extracting cream and water extraction cream are merged, add ethanol precipitation secondary, refrigerate 5 days for the first time, refrigerate 3 days for the second time, get the supernatant Recycled ethanol after, for subsequent use; After getting the honey thin up, be heated to and dissolve, to clarification, for subsequent use with diatomite filtration; Merge gained liquid after the above-mentioned refrigeration, add honey for subsequent use, the distilled water that adds again 5 times of raw material gross weights is mixed well, and transfers pH to 10.0 to filter with NaOH solution; With the gained filled with solution, every 10ml, after the sealing 120 ℃ of lower pressure sterilizings 35 minutes, both.
Embodiment 5:
The present invention increases the immunity function experimental study
1. material
1.1 sample: provided by S﹠P Pharmaceutical Co., Ltd..
1.2 animal used as test
160 of Kunming mouses, entirely female, body weight 18-22g.
2. experimental technique
2.1 dosage is selected
Tested material is established 400mg/kg.BW, 800mg/kg.BW, three dosage groups of 1200 mg/kg.BW (be equivalent to respectively human body recommended intake 10 times, 20 times, 30 times), take by weighing respectively 10.00g, 20.00g, 30.00g tested material adding distil water to the 250ml mixing, stored refrigerated, be finished again and join, other establishes the edible vegetable oil negative control group, every group of 40 animals.Be divided into immune one group, two groups, three groups, four groups, wherein one group is used for Turnover of Mouse Peritoneal Macrophages and engulfs the chicken red blood cell experiment; Two groups are used for NK cytoactive detection and lymphocyte transformation experiment; Three groups are used for antibody-producting cell experiment, serum hemolysin mensuration and delayed allergy; Four groups are used for mouse carbon and clean up experiment.Mouse gives 30 days continuously by per os gavage of 10 mg/kg.BW body weight, claims weekly body weight one time, adjusts the gavage volume.
2.2 experimental procedure
2.2.1 mouse carbon clearance test: gavage is after 30 days continuously, in the india ink of mouse tail vein injection with 4 times of normal saline dilutions, immediately timing after pressing 0.1ml/10g prepared Chinese ink and injecting is after injecting prepared Chinese ink the 2nd, 10min gets blood 20 μ l from the angular vein clump respectively, is added to 2mlNa
2CO
3In the solution, with Na
2CO
3Solution is made blank, measures OD value at the 600nm place with 723 spectrophotometers.Put to death mouse after getting blood, get liver, spleen is weighed, calculate phagocytic index.
2.2.2 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (half intracorporal method): gavage is after 30 days continuously, the chicken erythrocyte suspension 1ml of every mouse peritoneal injection 20%, after 30 minutes, the cervical vertebra dislocation is put to death, be fixed on the mouse plate, abdominal skin is cut off in the center, through Intraperitoneal injection physiological saline 2ml, rotated the mouse plate 1 minute, sucking-off abdominal cavity washing lotion 1ml, mean droplet is on 2 slides, put 37 ℃ in wet box 30 minutes, and took out in the physiological saline rinsing, dry, fixing, 4%GiemsaPBS dyeing 3 minutes, the distilled water rinsing is dried, microscopy.Calculate phagocytic percentage and phagocytic index.
2.2.3 delayed allergy (DTH) (the sufficient sole of the foot thickens method): gavage is after 30 days, inject the every mouse of 2% hematocrit SRBC(0.2ml/ to mouse peritoneal) sensitization is after 4 days, measure left back sufficient sole of the foot thickness, and then measuring point hypodermic injection 20%(v/v) the every mouse of SRBC(20 μ l/), 24h measures left back sufficient sole of the foot thickness after injection, same position is measured three times, averages, and represents the degree of DTH with sufficient sole of the foot thickness difference (swelling degree of the paw) before and after attacking.
2.2.4ConA the mouse lymphocyte conversion test (mtt assay) of inducing: at first carry out the preparation of splenocyte suspension.Cell concentration is adjusted into 3 * 10
6Individual/ml, then cell suspension is divided two holes to add in 24 well culture plates, every hole 1ml, a hole adds 75 μ lConA liquid, and another hole compares, and puts 5%CO
237 ℃ of cultivations of incubator 72h.Cultivate and finish front 4h, every hole sucks gently supernatant 0.7ml and adds the RPMI1640 nutrient solution that 0.7ml does not contain calf serum, add simultaneously MTT (5mg/ml) 50 μ l/ holes and continue to cultivate 4h, after cultivating end, every hole adds 1ml acid isopropyl alcohol, purple crystal is dissolved fully, then every hole liquid is moved in the cuvette, with 723 spectrophotometers in 570nm wavelength place's colorimetric estimation OD value.
2.2.5 antibody-producting cell detects (Jernr improves slide method): gavage is after 30 days continuously, every mouse peritoneal is injected 2% hematocrit SRBC0.2ml, the mouse cervical vertebra dislocation of immunity after 5 days half put to death, take out spleen and be placed in the plate that fills Hank ' s liquid, make splenocyte suspension.Agarose is made into 1% aqueous solution, and 30min is boiled in water-bath, mix with the double Hank ' s of equivalent liquid, and the packing small test tube, every pipe 0.5ml adds 10%(S again in pipe
AThe buffer solution preparation) hematocrit SRBC50 μ l, each 20 μ l of splenocyte suspension do two Duplicate Samples, rapidly behind the mixing, be poured on the agarose thin layer slide, after agar solidifies, the slide level buckled be placed on the horse, put into CO2gas incubator and hatch 1.5h, then complement is joined in the slide frame groove, continue incubation 1.5h, counting hemolysis plaque number.
2.2.6 serum hemolysin is measured (Hemagglutination Method): in continuous gavage after 30 days, every mouse peritoneal injection 2%SRBC0.2ml immunity, continue gavage after 5 days, extract eyeball and get blood in centrifuge tube, place 1h, peel off, the centrifugal 10min of 2000r/min collects serum, with physiological saline serum is made doubling dilution, every part of dilution 12 holes place blood-coagulation-board with the dilution serum of difference, every hole 100 μ l, add again 0.5%SRBC suspension 100 μ l, mixing is put observed result behind wet 37 ℃ of 3h of box, records the aggegation degree in every hole.The calculating antibody product.
2.2.7NK cytoactive detection (lactate dehydrogenase L DH determination method): continuously gavage is after 30 days, and animal is put to death in the cervical vertebra dislocation, takes out spleen, tears up, cross 200 eye mesh screens after, use Hank ' s liquid to wash 3 times, be made into 2 * 10 with complete RPMI1640 nutrient solution
7Individual/the ml cell suspension.The cell suspension of each mouse is got 300 μ l divide 3 holes to place 96 well culture plates, every hole 100 μ l, every hole adds target cell (YAC-1 cell, 4 * 10
5Individual/ml) 100 μ l, do simultaneously each 8 hole of target cell Spontaneous release hole (target cell 100 μ l+ nutrient solutions 100 μ l) and maximum release aperture (target cell 100 μ l+2.5%Triton100 μ l), 37 ℃ of 5%CO
2Cultivated 4 hours, and took out the centrifugal 5min of 1500r/min.Each hole supernatant 100 μ l is placed another culture plate, and every hole adds 100 μ l matrix liquid again, adds the HCL30 μ l cessation reaction of 1mol/L after 10 minutes, measures the OD value at the 490nm place, calculates NK cytoactive rate.
3. test data is added up
Test data adopts SPSS10.0 for Windows software kit to process.The data of control group and dosage group are through the variance test of homogeneity, and variance is neat, carry out variance analysis,, then compare in twos with the Dunnett method less than 0.05 such as the P value; If heterogeneity of variance then carries out data transaction, and is still uneven, use rank test instead, less than 0.05, then use Dunnett ' sT3 method to compare in twos (P〉0.05 for non-significant difference, P<0.05 is significant difference) such as the P value.
4. result
4.1 the present invention sees Table 1-4 to the impact of Mouse Weight.
Table 1 respectively organize mouse initial body weight (
)
Table 2 respectively organize mouse the body weight in mid-term (
)
Table 3 respectively organize mouse the end body weight (
)
The impact that table 4 the present invention increases weight on Mouse Weight (
)
By table 1-4 as seen, the initial body weight of one group, immune two groups of the present invention immunity, immune three groups and immune four groups of mouse is compared with negative control group, through the variance test of homogeneity, variance neat (P〉0.05), and the results of analysis of variance (P〉0.05), illustrate that the initial body weight of respectively organizing between mouse and the negative control group is balanced.Three dosage group mouse test mid-terms, the body weight in latter stage and the growths of duration of test Mouse Weight are compared with negative control group, learn by statistics and process, there was no significant difference (P〉0.05), i.e. the present invention on the body weight gain of mouse without impact.
4.2 the present invention is on the impact of mouse monokaryon-macrophage phagocytic function
Table 5 the present invention on mouse monokaryon-macrophage carbon clean up function impact (
)
By as seen from Table 5, gavage is after 30 days continuously, and the carbon of three dosage group mouse is cleaned up ability and compared with negative control group, and learn by statistics and process, there was no significant difference (P〉0.05).
Table 6 the present invention on mouse macrophage engulf the chicken red blood cell ability impact (
)
By as seen from Table 6, gavage is after 30 days continuously, and the phagocytic rate of three dosage treated animals is compared with negative control group with phagocytic index, learns by statistics and processes, and middle and high dosage group phagocytic index has significant difference (P<0.05).
By table 5, as seen from Table 6, the present invention is positive to mouse monokaryon-macrophage phagocytic function test result.
4.3 the present invention is on the impact of mouse cell immunity
Table 7 the present invention on the impact of mouse delayed allergy (DTH) (
)
By as seen from Table 7, gavage is after 30 days continuously, and the swelling degree of the paw of three dosage group mouse is compared with negative control group, and learn by statistics and process, there was no significant difference (P〉0.05)
The impact of the mouse lymphocyte conversion test that table 8 the present invention induces ConA (
)
By as seen from Table 8, the gavage mouse is after 30 days continuously, and the lymphopoiesis ability of three dosage group mouse is compared with negative control, and learn by statistics and process, there was no significant difference (P〉0.05).
By table 7, the present invention is negative to mouse cell immunity test result as seen from Table 8.
4.4 the present invention is on the impact of mouse humoral immune
Table 9 the present invention on the impact of mouse antibodies cellulation (
)
By as seen from Table 9, gavage is after 30 days continuously, and the hemolysis plaque number of three dosage group mouse is compared with negative control, learns by statistics and processes, and low, middle dosage group has significant difference (P<0.05).
Table 10 the present invention on the impact of mice serum hemolysin (
)
By as seen from Table 10, gavage is after 30 days continuously, and the antibody product of three dosage group mouse is compared with negative control group, learns by statistics and processes, and low dose group has utmost point significant difference (P<0.05)
By table 9, as seen from Table 10, the present invention is positive to the mouse humoral immune result of the test.
4.5 the present invention is on the impact of NK cells in mice activity
Table 11 the present invention on the impact of NK cells in mice activity (
)
By as seen from Table 11, gavage is after 30 days continuously, and three dosage group NK cells in mice activity are compared with negative control, and learn by statistics and process, there was no significant difference (P〉0.05).The present invention is aobvious negative to the NK cytoactive result of the test of mouse.
5. sum up
The continuous gavage mouse of the present invention had no significant effect Mouse Weight after 30 days; Test for celluar immunity result to mouse is negative; NK test cell line result to mouse is negative; To the test for humoral immunity of mouse, the hemolysis plaque number of three dosage groups and negative control group compare, and low, middle dosage group difference all has conspicuousness (P<0.05), and result of the test is positive; To the monocytes/macrophages phagocytic function test of mouse, the phagocytic rate of three dosage group mouse and phagocytic index and negative control group compare, and middle and high dosage group difference has conspicuousness (P<0.05), and result of the test is positive.
Can judge according to " health food check and assessment technique standard " version in 2003, the present invention has the function that increases immunity to animal.
Embodiment 6:
Around table 12 administration of the present invention on the impact (x ± s, n=10) of hyperlipidemia rats hemorheology index
Conclusion: of the present invention group can significantly be reduced the hyperlipidemia rats plasma viscosity, and packed cell volume (HCT) WBV is not made significant difference.
Can judge according to " health food check and assessment technique standard " version in 2003, the present invention has the function that reduces blood fat to animal.