CN102813057A - Method for producing fermented feed through utilizing enteromorpha leaching liquor - Google Patents
Method for producing fermented feed through utilizing enteromorpha leaching liquor Download PDFInfo
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- CN102813057A CN102813057A CN2012103474049A CN201210347404A CN102813057A CN 102813057 A CN102813057 A CN 102813057A CN 2012103474049 A CN2012103474049 A CN 2012103474049A CN 201210347404 A CN201210347404 A CN 201210347404A CN 102813057 A CN102813057 A CN 102813057A
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- enteromorpha
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Abstract
The invention belongs to the field of microbial fermented feeds, and in particular relates to a method for producing a fermented feed through utilizing enteromorpha leaching liquor, wherein dry enteromorpha which is coarsely ground is soaked in the water, then uniformly mixed and separated; then, the mixture is mixed: feed, fermented strain and the enteromorpha leaching liquor are put in a blender mixer to be mixed, and then, the mixed material is uniformly discharged; and finally, the mixed material is fermented: the mixed material is uniformly laid in a room, subjected to thermal insulation treatment, fermented for 37-72 hours and dried, and thus, the microbial fermented feed is produced. The method has the advantages that the activity of the compound strain in the fermented feed can be stimulated, and the nutritional ingredients of the feed, which cannot be easily absorbed by livestock directly, are degraded into micromolecular substances which can be absorbed and digested, such as various amino acids, small peptides and the like; and moreover, the number of putrefying bacteria in the feed is reduced, and the probability of causing digestive canal diseases after the livestock eat the feed is decreased.
Description
Technical field
The invention belongs to the fermentative feedstuff of microbe field, be specifically related to a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed.
Background technology
Along with the fast development of livestock breeding industry, the contradiction that the people holds common grain is outstanding day by day, and research has become the emphasis of people's research with utilizing the new way of developing feed resource, and wherein fermentative feedstuff of microbe more and more comes into one's own.
Fermentative feedstuff of microbe is meant under artificial controllable condition; With the vegetalitas agricultural byproducts is primary raw material; Metabolism through microorganism; Macromolecular substances such as degraded part polysaccharide, protein and fat generate small-molecule substances such as organic acid, soluble polypeptide, form nutritious, good palatability, biological feedstuff or feedstuff that useful viable bacteria content is high.Microorganism is just decomposed nutriments such as the glucose of feed, protein during the fermentation in advance; Help domestic animal feed absorption, digestion afterwards; There is a large amount of probios to exist simultaneously in the fermented feed; Can suppress the harmful bacteria in the feed, reduce the probability of the gastral disease generation of domestic animal, this is unexistent in the conventional feed.And fermented feed has fermenting aroma, good palatability, and livestock is liked to eat.
Enteromorpha is a genus of Chlorophyta Ulvaceae, and Enteromorpha self is rich in carbohydrate, protein, crude fibre and mineral matter, also contains fat and vitamin simultaneously.And Enteromorpha can reduce the pH value of feed moisture; Regulate the metabolic process of microorganism; If can Enteromorpha be used for the production of fermentative feedstuff of microbe, will promote the activity of bacterial classification in the fermented feed so, improve the fermentation usefulness of feed; Reduce antibiotic use in the domestic animal nursing, the security that has improved food.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed; Biochemical reaction through the Enteromorpha leaching liquor can effectively improve the nutritional labeling in the fermented feed; Shortened the feed fermentation time; Reduce the putrefactivebacteria amount in the fermented feed, thereby guaranteed the quality of fermented feed.
In order to realize above technique effect, the present invention realizes through following steps:
(1) preparation of Enteromorpha leaching liquor: the dry Enteromorpha after the coarse crushing is immersed in the water, evenly stir the back then and separate;
(2) mixing of mixed material: feed, fermented bacterium and Enteromorpha leaching liquor are put into batch mixer stir, mixed material is discharged after evenly;
(3) fermentation of mixed material: mixed material evenly is layed in indoor, and is incubated processing, it is dry with mixed material after 37-72 hour to ferment.
In the said step (1), with dry Enteromorpha coarse crushing to 40 order-50 order, the moisture content 6%-10% of said dry Enteromorpha.
In the said step (1), mix dry Enteromorpha with the weight proportion of water by 1:900-1100, be heated to 45 ℃-50 ℃ and slowly at the uniform velocity stirring, Separation of Solid and Liquid is got the Enteromorpha leaching liquor after 1-1.5 hour.
Fermented bacterium in the said step (2) is one or more of bifidocaterium, lactic acid bacteria, bacillus, photosynthetic bacteria, saccharomycete and aspergillus niger, and wherein zymophyte content is 1 * 10
9Cfu/ml-1 * 10
10Cfu/ml.
In the said step (2), feed, fermented bacterium and Enteromorpha leaching liquor according to 1kg:0.4 * 10
9Cfu-2 * 10
10Cfu:0.5kg-1kg mixes, and incorporation time is 5-10 minute, and described feed is the dish dregs of rice, dregs of beans or cotton dregs.
In the said step (2), add 0.08 ‰-0.34 ‰ auxiliary leavening of mixed material total amount during to 2-4 minute at the batch mixer mixed material.
Said auxiliary leavening is a sucrose.
In the said step (3), be 5-10 rice with mixed material according to length, width is a 1.5-2 rice, highly evenly is layed on the indoor plank or concrete floor for 0.2-0.3 rice.
The method that insulation in the said step (3) is handled does, on mixed material, builds the insulation canopy with film, and this insulation canopy is a 1-1.5 rice apart from the height of mixed material.
Be incubated processing down at 10 ℃-35 ℃ in the said step (3).
The invention has the beneficial effects as follows: the present invention is a kind of technology of production fermented feed of technical process improvement; Add the Enteromorpha leaching liquor in process of production; Because Enteromorpha itself just is rich in nutriments such as protein, carbohydrate, vitamin; And Enteromorpha also can promote the activity of the composite bacteria in the fermented feed; Can better being not easy in the feed be resolved into the small-molecule substance that can be absorbed, digest by the nutritional labeling that domestic animal directly absorbs,, can effectively guarantee the fine quality of fermented feed like this like each amino acid, little peptide etc.; Simultaneously can also reduce the putrefactivebacteria number in the feed, reduce domestic animal at the incidence rate that eats the disease of digestive tract that is caused the back, thereby reduce antibiotic use amount, improve the security of the carnivorous food of domestic animal.
The specific embodiment
Below in conjunction with embodiment, the present invention is described further:
Embodiment 1
Feed is a dregs of beans:
To be crushed to the dry Enteromorpha 0.5kg of 40 purposes (water content of this drying Enteromorpha is 10%) inserts in the heated type mixer and adds 500kg water; Mixer stirs at a slow speed; This splash bar rotating speed is 5rpm/ minute; When stirring, heat, constant temperature stirs when being heated to 45 ℃, and heated and stirred continues 1 hour.Mixed liquor is fed Rose Box separate with filter cloth, the leaching liquor after the separation is faint yellow, and it is subsequent use to pour into container.With 1000kg dregs of beans, 0.5L composite zymocyte, wherein zymophyte content is 9 * 10
9Cfu/ml-12 * 10
9Cfu/ml.(the special-purpose EM stoste of the breed that the wide space in Anhui Bioisystech Co., Ltd produces; Its main component is bifidocaterium, lactic acid bacteria, bacillus, photosynthetic bacteria, saccharomycete) and 500kg Enteromorpha leaching liquor put into batch mixer and stir; Agitator shaft speed is 20rpm/ minute; Pour 0.5kg sucrose when being mixed to 2 minutes into and continue to mix, mixing of materials is discharged after being mixed to 8 minutes.
The specification of mixed material according to 0.3 meter of 7 meters of length, 1.5 meters of width, height is layed on the indoor cement flooring, builds the insulation canopy with transparent plastic film then, changing the insulation canopy is 1 meter apart from the height of mixed material.Fermentation time is 48 hours, and the dregs of beans that advances behind the everfermentation has little acid gas flavor, and moisture content is 32%.Fermentation removes the insulation canopy after accomplishing, and carries out stored dry after fermented bean dregs is collected.
Nutritional labeling contrast (table one) in Enteromorpha leaching liquor fermented bean dregs and the traditional zymotic dregs of beans:
| Dregs of beans | The traditional zymotic dregs of beans | The leaching liquor fermented bean dregs | |
| Crude protein % | 41.3 | 50.5 | 51.6 |
| Crude fibre % | 5.8 | 3.3 | 3.1 |
| Little peptide content % | 0.5 | 8.1 | 9.8 |
| VBN mg/100g | 6.8 | 7.7 | 7.4 |
Can know that from table one utilize the dregs of beans of Enteromorpha leaching liquor fermentation to rise 2% than the crude protein content in the traditional zymotic dregs of beans, little peptide content has risen 21%, VBN (mg/100g) content has descended 5.2%, and crude fibre has reduced by 6.1%.
Embodiment 2
Feed is cotton dregs
To be crushed to the dry Enteromorpha 1kg of 40 purposes (moisture content of this drying Enteromorpha is 10%) inserts the heated type mixer and adds 1000kg water.Mixer stirs at a slow speed, and the splash bar rotating speed is 5rpm/ minute, heats simultaneously, and constant temperature stirs when being heated to 50 ℃, and heated and stirred continues 1 hour.Then, mixed liquor is fed Rose Box with filter cloth Enteromorpha is separated with leaching liquor, the leaching liquor after the separation is faint yellow, and it is subsequent use to pour into container.(wherein zymophyte content 5 * 10 with 2000kg cotton dregs, 2L aspergillus niger and lactic acid bacteria composite bacteria
9Cfu/ml-2 * 10
10Cfu/ml) and 1000kg Enteromorpha leaching liquor stir according to feeding mixer together, agitator shaft speed is 15rpm/ minute, pours 0.8kg sucrose when being mixed to 2 minutes into and proceeds to mix, and mixed material is discharged after being mixed to 10 minutes.The specification of mixed material according to 0.3 meter of long 10 meters, 2 meters of width, height is layed on the indoor cement flooring, builds the insulation canopy with transparent plastic film then, changing the insulation canopy is 1 meter apart from the height of mixed material.Fermentation time is 72 hours, and the cotton dregs that advance behind the everfermentation have little acid gas flavor, and moisture content is 35%.Fermentation removes the insulation canopy after accomplishing, and the cotton dregs that will ferment carry out stored dry after collecting.
Nutritional labeling contrast (table two) in Enteromorpha leaching liquor fermentation cotton dregs and the traditional zymotic cotton dregs:
| Cotton dregs | The traditional zymotic cotton dregs | Leaching liquor fermentation cotton dregs | |
| Crude protein % | 39.2 | 42.1 | 44.6 |
| Crude fibre % | 12.3 | 9.5 | 9.1 |
| Lysine % | 1.56 | 1.72 | 1.77 |
Can know that from table two risen 6% than the crude protein content in the traditional zymotic cotton dregs in the cotton dregs that utilize the Enteromorpha leaching liquor to ferment, lysine content has risen 3%, crude fiber content has reduced by 4.2%.
Embodiment 3
Feed is the dish dregs of rice
To be crushed to the dry Enteromorpha 0.3kg of 40 purposes (moisture content of this drying Enteromorpha is 10%) inserts the heated type mixer and adds 300kg water.Mixer stirs at a slow speed, and the splash bar rotating speed is 8rpm/ minute, heats simultaneously, and constant temperature stirs when being heated to 50 ℃, and heated and stirred continues 1 hour.Then, mixed liquor is fed Rose Box with filter cloth Enteromorpha is separated with leaching liquor, the leaching liquor after the separation is faint yellow, and it is subsequent use to pour into container.With the 600kg dish dregs of rice, 0.3L composite bacteria liquid, wherein zymophyte content is 9 * 10
9Cfu/ml-2 * 10
10Cfu/ml, (its composition is saccharomycete, bacillus and lactic acid bacteria) and 600kg Enteromorpha leaching liquor stir according to feeding mixer together; Agitator shaft speed is 20rpm/ minute; Pour 0.1kg sucrose when being mixed to 2 minutes into and continue to mix, mixed material is discharged after being mixed to 10 minutes.The specification of mixed material according to 5 meters of length, 1.5 meters of width, 0.2 meter of height is layed on the indoor cement flooring, builds the insulation canopy with transparent plastic film then, this insulation canopy is 1 meter apart from the height of mixed material.Fermentation time is 37 hours, and the dish dregs of rice that advance behind the everfermentation have little acid gas flavor, and moisture content is 38%.Fermentation removes the insulation canopy after accomplishing, and the dish dregs of rice that will ferment carry out stored dry after collecting.
Nutritional labeling contrast (table three) in the Enteromorpha leaching liquor fermentation dish dregs of rice and the traditional zymotic dish dregs of rice:
| The dish dregs of rice | The traditional zymotic dish dregs of rice | The leaching liquor fermentation dish dregs of rice | |
| Crude protein % | 35.8 | 40.1 | 41.3 |
| Crude fibre % | 11.1 | 8.4 | 8.2 |
| Lysine % | 1.4 | 1.66 | 1.67 |
Can know that from table three utilize the dish dregs of rice of Enteromorpha leaching liquor fermentation to rise 3% than the crude protein content in the traditional zymotic dish dregs of rice, lysine content has risen 1%, crude fiber content has reduced by 2.3%.
So; From the nutritional labeling contrast of above-mentioned three tables, can learn; Utilize the crude protein, the little peptide content of lysine etc. of the feed ratio traditional zymotic feed of Enteromorpha leaching liquor fermentation all to increase; Then do not descended by the crude fibre that domestic animal absorbed, the nutritional labeling of this explanation employing fermented feed that the present invention produced is horn of plenty more.
Claims (10)
1. method of utilizing the Enteromorpha leaching liquor to produce fermented feed, its step comprises:
(1) preparation of Enteromorpha leaching liquor: the dry Enteromorpha after the coarse crushing is immersed in the water, evenly stir the back then and separate;
(2) mixing of mixed material: feed, fermented bacterium and Enteromorpha leaching liquor are put into batch mixer stir, mixed material is discharged after evenly;
(3) fermentation of mixed material: mixed material evenly is layed in indoor, and is incubated processing, it is dry with mixed material after 37-72 hour to ferment.
2. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 1 is characterized in that: in the said step (1), with dry Enteromorpha coarse crushing to 40 order-50 order, the moisture content 6%-10% of said dry Enteromorpha.
3. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 1; It is characterized in that: in the said step (1); Mix dry Enteromorpha with the weight proportion of water by 1:900-1100; Be heated to 45 ℃-50 ℃ and slowly at the uniform velocity stirring, Separation of Solid and Liquid is got the Enteromorpha leaching liquor after 1-1.5 hour.
4. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 1; It is characterized in that: the fermented bacterium in the said step (2) is one or more in bifidocaterium, lactic acid bacteria, bacillus, photosynthetic bacteria, saccharomycete and the aspergillus niger, and wherein zymophyte content is 1 * 10
9Cfu/ml-1 * 10
10Cfu/ml.
5. according to claim 1 or 4 described a kind of methods of utilizing the Enteromorpha leaching liquor to produce fermented feed, it is characterized in that: in the said step (2), feed, fermented bacterium and Enteromorpha leaching liquor according to 1kg:0.4 * 10
9Cfu-2 * 10
10Cfu:0.5kg-1kg mixes, and incorporation time is 5-10 minute, and described feed is the dish dregs of rice, dregs of beans or cotton dregs.
6. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 5 is characterized in that: in the said step (2), add 0.08 ‰-0.34 ‰ auxiliary leavening of mixed material total amount during to 2-4 minute at the batch mixer mixed material.
7. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 6, it is characterized in that: said auxiliary leavening is a sucrose.
8. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 1; It is characterized in that: in the said step (3); Is 5-10 rice with mixed material according to length, and width is a 1.5-2 rice, highly evenly is layed on the indoor plank or concrete floor for 0.2-0.3 rice.
9. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 1; It is characterized in that: the method that the insulation in the said step (3) is handled does; On mixed material, build the insulation canopy with film, this insulation canopy is a 1-1.5 rice apart from the height of mixed material.
10. a kind of method of utilizing the Enteromorpha leaching liquor to produce fermented feed according to claim 1 is characterized in that: said step (3) is 10 ℃-35 ℃ insulation processing down.
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| CN201210347404.9A CN102813057B (en) | 2012-09-17 | 2012-09-17 | Method for producing fermented feed through utilizing enteromorpha leaching liquor |
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| CN201210347404.9A CN102813057B (en) | 2012-09-17 | 2012-09-17 | Method for producing fermented feed through utilizing enteromorpha leaching liquor |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103352016A (en) * | 2013-06-27 | 2013-10-16 | 中国海洋大学 | Method for preparing biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha |
| CN103931886A (en) * | 2014-04-11 | 2014-07-23 | 集美大学 | Enteromorpha prolifera feed additive as well as preparation method and applications thereof |
| CN106343175A (en) * | 2016-08-29 | 2017-01-25 | 界首市任寨乡天佑家庭农场 | Fodder used for producing functional beef high in DHA content |
| CN106490307A (en) * | 2016-12-14 | 2017-03-15 | 河南牧翔动物药业有限公司 | A kind of method of antimicrobial composition fermentation Entermorpha |
| CN115067424A (en) * | 2022-07-06 | 2022-09-20 | 福州天凯生物科技有限责任公司 | Enteromorpha fermentation preparation process |
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| CN1181365A (en) * | 1997-11-21 | 1998-05-13 | 董巧云 | Method for preparing animal feed or fertilizer |
| AU2007200687A1 (en) * | 2006-02-23 | 2007-09-06 | Van Schaik's Bio Gro Pty Ltd | A seagrass based nutrient product for plant biological growth stimulation and a method for preparing the same |
| CN101669580A (en) * | 2009-09-21 | 2010-03-17 | 宁波大学 | Method for preparing feed additive by fermenting enteromorpha |
| CN102613400A (en) * | 2012-02-21 | 2012-08-01 | 山东大学威海分校 | Optimum processing technology of enteromorpha additive aquatic feeds |
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2012
- 2012-09-17 CN CN201210347404.9A patent/CN102813057B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168773A (en) * | 1997-04-30 | 1997-12-31 | 窦观一 | Fermanted algae paste and the use thereof |
| CN1181365A (en) * | 1997-11-21 | 1998-05-13 | 董巧云 | Method for preparing animal feed or fertilizer |
| AU2007200687A1 (en) * | 2006-02-23 | 2007-09-06 | Van Schaik's Bio Gro Pty Ltd | A seagrass based nutrient product for plant biological growth stimulation and a method for preparing the same |
| CN101669580A (en) * | 2009-09-21 | 2010-03-17 | 宁波大学 | Method for preparing feed additive by fermenting enteromorpha |
| CN102613400A (en) * | 2012-02-21 | 2012-08-01 | 山东大学威海分校 | Optimum processing technology of enteromorpha additive aquatic feeds |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103352016A (en) * | 2013-06-27 | 2013-10-16 | 中国海洋大学 | Method for preparing biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha |
| CN103931886A (en) * | 2014-04-11 | 2014-07-23 | 集美大学 | Enteromorpha prolifera feed additive as well as preparation method and applications thereof |
| CN103931886B (en) * | 2014-04-11 | 2016-08-24 | 集美大学 | A kind of enteromorpha feed additive, preparation method and its usage |
| CN106343175A (en) * | 2016-08-29 | 2017-01-25 | 界首市任寨乡天佑家庭农场 | Fodder used for producing functional beef high in DHA content |
| CN106490307A (en) * | 2016-12-14 | 2017-03-15 | 河南牧翔动物药业有限公司 | A kind of method of antimicrobial composition fermentation Entermorpha |
| CN115067424A (en) * | 2022-07-06 | 2022-09-20 | 福州天凯生物科技有限责任公司 | Enteromorpha fermentation preparation process |
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| CN102813057B (en) | 2014-11-26 |
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