CN102764285A - Coreopsis tinctoria extract - Google Patents
Coreopsis tinctoria extract Download PDFInfo
- Publication number
- CN102764285A CN102764285A CN2012102707997A CN201210270799A CN102764285A CN 102764285 A CN102764285 A CN 102764285A CN 2012102707997 A CN2012102707997 A CN 2012102707997A CN 201210270799 A CN201210270799 A CN 201210270799A CN 102764285 A CN102764285 A CN 102764285A
- Authority
- CN
- China
- Prior art keywords
- extract
- dichromatism
- coreopsis basalis
- coreopsis
- basalis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a coreopsis tinctoria extract, which is obtained by the following steps of: grinding coreopsis tinctoria; adding ethanol aqueous solution with mass concentration of 15-95%, wherein the feed liquid ratio is 1: (10-25); after soaking for 1-12h, refluxing and extracting for 1-3h at 40-90 DEG C for 1-3 times; filtrating; mixing the filtrate; decompressing and condensing; and drying to obtain the extract. The coreopsis tinctoria extract obtained by the invention has the capacity of removing DPPH (1,1-dipheny 1-2-picrylhydrazyl) radicals and hydroxy radicals better, wherein the EC50 (concentration for 50% of maximal effect) for removing the hydroxyl radicals is 0.307mg/ml and is superior to 0.43mg/ml of vitamin C, the EC50 of an extracting stock solution is 0.101mg/ml, therefore, the coreopsis tinctoria extract can be used for preparing the antioxidative functional foods or medicines.
Description
Technical field
The invention belongs to the extract drugs field, be specifically related to a kind of dichromatism Coreopsis basalis extract.
Background technology
The equal generic several kinds commonly used that have of Coreopsis basalis, China's cultivar mainly contains: dichromatism Coreopsis basalis (Coreopsis tinctoria Nutt.) has another name called Herba Coreopsis tinctoria, money chrysanthemum; Flos Caryophylli Coreopsis basalis (C. Grandifloa Hogg); Sword-like leave Coreopsis basalis (C. Lanceolata L.) has another name called Coreopsis lanceolata.
Relevant bibliographical information shows: detect saccharide, organic acid, flavone, lactone, steroidal, triterpenes and volatile oil isoreactivity composition in the Coreopsis basalis, and saponin, anthraquinone, coumarin, cardiotonic glycoside and alkaloid do not detect.And the pistil of Coreopsis basalis becomes brown, and test shows that this pigment belongs to water colo(u)r, and solution is subacidity, does not contain alkaloid, and supposition should contain abundant anthocyanidin.
Coreopsis basalis pattern pool is bright-coloured, can be used for extracting flavochrome, and Coreopsis basalis flavochrome main component is a flavone compound.Result of the tests such as Chaud Doc show that Coreopsis basalis contains abundant flavonoids.Chinese scholars research shows that the pigment composition in the Coreopsis basalis is that filament is plain and Coreopsis lanceolata is plain.Fine rule in the Flos Caryophylli Coreopsis basalis plain and chalcone glycosides difference Shu aurone class and chalcones flavonoids, through identifying, the chrysanthemum flavin contains fine rule plain 1 ‰ approximately.
Water-soluble and the ethanol of chrysanthemum flavochrome that from the inflorescence of Coreopsis lanceolata, extracts; Light resistance and thermostability are all better; This pigment < was yellow at 7 o'clock at pH; Color and luster is more stable, is the different glycosides of Flos Caryophylli Coreopsis basalis (Leptosin), Coreopsis lanceolata different (Leptosidln), Coreopsis lanceolata chalcone (Laneeoletin) and Coreopsis lanceolata chalcone glycosides flavone compounds such as (Lanceolin) through the main coloring ingredient of this pigment of isolation identification.
The dichromatism Coreopsis basalis, dry head's inflorescence of genus Compositae golden pheasant Chrysanthemum, ligulate flower is yellow or golden yellow, 6～JIUYUE of florescence.
Summary of the invention
The purpose of this invention is to provide a kind of dichromatism Coreopsis basalis extract.
The present invention realizes that the technical scheme that above-mentioned purpose adopts is following:
A kind of dichromatism Coreopsis basalis extract, this extract are to be 15～95% ethanol water through the dichromatism Coreopsis basalis being pulverized, adding mass concentration; Solid-liquid ratio is 1: (10～25), soak after 1～12 hour, extracted 1～3 hour 40～90 ℃ of refluxed; Reflux, extract, 1～3 time; Filter, merging filtrate obtains through concentrating under reduced pressure, drying.
Further, the mass concentration of said ethanol water is 50～60%.
Further, said solid-liquid ratio is 1: (12～18).
Further, soaked 2～3 hours.
Further, the reflux, extract, temperature is 45～55 ℃.
Further, the said dry lyophilization of adopting, baking temperature is-5～-55 ℃.
Further, said baking temperature is-20～-55 ℃.
Preferred, said baking temperature is-45～-55 ℃.
Prepreerence, said baking temperature is-51～-53 ℃.
Further, the application of said dichromatism Coreopsis basalis extract in preparation antioxidative functional food or medicine.
Beneficial effect
Gained dichromatism Coreopsis basalis extract tool of the present invention is removed the ability of DPPH free radical and hydroxyl radical free radical preferably, wherein, removes the EC of hydroxyl radical free radical
50Be 0.307mg/ml, be superior to ascorbic 0.43mg/ml, extract the EC of stock solution
50Be 0.101mg/ml.Characteristics such as method for distilling of the present invention has the extraction ratio height, and is easy to operate, and cost is low.
The specific embodiment
Below in conjunction with embodiment the present invention is further specified.
Embodiment 1
Take by weighing dichromatism golden pheasant Flos Chrysanthemi 100g, pulverized 60 mesh sieves, the mass concentration that adds 18 times of amounts (by the Mass Calculation of dichromatism golden pheasant Flos Chrysanthemi) is 55% ethanol; After soaking 3h, 55 ℃ of reflux, extract, 2.5h filter; Filter cake use the mass concentration of 18 times of amounts be 55% ethanol in 55 ℃ of reflux, extract, 2.5h, filter waste; Merging filtrate (extraction ratio 32%); Concentrating under reduced pressure (Rotary Evaporators, 45 ± 2 ℃), lyophilization (53 ℃) obtain extract (extraction ratio 17%), and main component is a flavone compound.Mass concentration is that 55% ethanol adds the water preparation by 95% ethanol and obtains, and extraction ratio descends obviously after concentrating under reduced pressure, dried, and extraction ratio accounts for the mass percent of raw material dichromatism golden pheasant Flos Chrysanthemi in the gained total flavones.
Embodiment 2
Take by weighing dichromatism golden pheasant Flos Chrysanthemi 100g, pulverized 60 mesh sieves, the mass concentration that adds 15 times of amounts is 50% ethanol (solid-liquid ratio is 1:15); After soaking 2h, 50 ℃ of reflux, extract, 2h, reflux, extract, 3 times; Filter; Merging filtrate, filtrating obtains extract through concentrating under reduced pressure (Rotary Evaporators, 45 ± 2 ℃), lyophilization (51 ℃).
Embodiment 3
Take by weighing dichromatism golden pheasant Flos Chrysanthemi 100g, pulverized 60 mesh sieves, the mass concentration that adds 12 times of amounts is 60% ethanol; After soaking 2.5h, 45 ℃ of reflux, extract, 3h, reflux, extract, 2 times; Filter; Merging filtrate, filtrating obtains extract through concentrating under reduced pressure (Rotary Evaporators, 45 ± 2 ℃), lyophilization (52 ℃).
Embodiment 4
Take by weighing dichromatism golden pheasant Flos Chrysanthemi 100g, pulverized 60 mesh sieves, the mass concentration that adds 10 times of amounts is 95% ethanol; After soaking 1h, 90 ℃ of reflux, extract, 1h filter; Filtrating obtains extract through concentrating under reduced pressure (Rotary Evaporators, 45 ± 2 ℃), lyophilization (52 ℃).
Embodiment 5
Take by weighing dichromatism golden pheasant Flos Chrysanthemi 100g, pulverized 60 mesh sieves, the mass concentration that adds 25 times of amounts is 15% ethanol; After soaking 12h, 40 ℃ of reflux, extract, 3h, reflux, extract, 2 times; Filter; Merging filtrate, filtrating obtains extract through concentrating under reduced pressure (Rotary Evaporators, 45 ± 2 ℃), lyophilization (53 ℃).
Embodiment 6 measures the extract obtained antioxidant activity of embodiment 1
The DPPH method is measured antioxidant activity
It is the sample A solution that 55% dissolve with ethanol is mixed with series concentration that the dichromatism Coreopsis basalis extract of dry gained is used mass concentration; Get 1ml sample A solution; Adding 4ml concentration is 1 of 4mg/100mL, 1-diphenyl-2-trinitrophenyl-hydrazine alcoholic solution (DPPH solution), mix homogeneously; The dark place reaction was measured its absorbance (A after 30 minutes in the 516nm place
i); Blank is that the use mass concentration is 55% ethanol replacement sample A solution, adds DPPH solution, measures absorbance (A
0); (Vc) is the contrast experiment with vitamin C.
Getting extracting solution (be after the reflux, extract, but without the filtrating of concentrate drying) simultaneously, to use mass concentration be the sample B solution (pressing the densitometer of dichromatism Coreopsis basalis extract) that 55% dissolve with ethanol is mixed with series concentration, and all the other steps are the same.
Clearance rate S (%)=(1-(Ai-Aj)/A
0) * 100%
The absorbance of Aj sample and alcohol mixeding liquid when not adding DPPH.
The Vc antioxidant activity
The antioxidant activity of dry gained dichromatism Coreopsis basalis extract (sample A)
。
The antioxidant activity of dichromatism Coreopsis basalis extract (sample B) in the extracting solution
Draw the EC of Vc, dry extract (sample A), extracting solution (sample B) removing DPPH free radical by experimental result
50Be worth as follows:
The hydroxyl radical free radical method is measured antioxidant activity
The orthophenanthroline alcoholic solution of getting 1ml concentration and be 0.75mmol/L is in test tube; Adding 2mL concentration successively is PBS and the 1mL distilled water of the pH=7.40 of 0.2mol/L; Fully behind the mixing; Adding 1mL concentration is copperas solution (FeSO4) mixing of 0.75mmol/L, adds 1mL concentration again and be 0.01% hydrogen peroxide (H
2O
2), in 37 ℃ of water-bath 60min, survey solution absorbency at the 510nm place, the absorbance that is the damage pipe is A (damage); The absorbance that replaces the 1mL distilled water then to record sample with the 1mL sample solution is A (appearance); The absorbance that uses the 1mL distilled water to replace the 1mL hydrogen peroxide to record is A (not).
Clearance rate (%)=(A (appearance)-A (damage))/(A (not)-A (damage)) * 100%
The Vc antioxidant activity
The antioxidant activity of dry gained dichromatism Coreopsis basalis extract (sample A)
The antioxidant activity of dichromatism Coreopsis basalis extract (sample B) in the extracting solution
Draw the EC of Vc, dry extract (sample A), extracting solution (sample B) removing hydroxyl radical free radical by experimental result
50Be worth as follows:
。
The result shows that the prepared dichromatism Coreopsis basalis of the present invention extract has certain antioxidant activity, and hydroxyl radical free radical is removed and can the ascorbic effect of force rate will be got well.Simultaneously, will point out also that extracting solution is after concentrating under reduced pressure, dried, the ability that extract is removed free radical decreases.
Claims (7)
1. dichromatism Coreopsis basalis extract is characterized in that: this extract is to be 15～95% ethanol water through the dichromatism Coreopsis basalis being pulverized, adding mass concentration; Solid-liquid ratio is 1: (10～25), soak after 1～12 hour, extracted 1～3 hour 40～90 ℃ of refluxed; Reflux, extract, 1～3 time; Filter, merging filtrate obtains through concentrating under reduced pressure, drying.
2. according to the said dichromatism Coreopsis basalis of claim 1 extract, it is characterized in that: the mass concentration of said ethanol water is 50～60%.
3. according to the said dichromatism Coreopsis basalis of claim 1 extract, it is characterized in that: said solid-liquid ratio is 1: (12～18).
4. according to the said dichromatism Coreopsis basalis of claim 1 extract, it is characterized in that: soaked 2～3 hours.
5. according to the said dichromatism Coreopsis basalis of claim 1 extract, it is characterized in that: the reflux, extract, temperature is 45～55 ℃.
6. according to the said dichromatism Coreopsis basalis of claim 1 extract, it is characterized in that: the said dry lyophilization of adopting, baking temperature is-5～-55 ℃.
7. the purposes of the arbitrary said dichromatism Coreopsis basalis extract of claim 1 to 6 is characterized in that: the application in preparation antioxidative functional food or medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210270799.7A CN102764285B (en) | 2012-08-01 | 2012-08-01 | Coreopsis tinctoria extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210270799.7A CN102764285B (en) | 2012-08-01 | 2012-08-01 | Coreopsis tinctoria extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102764285A true CN102764285A (en) | 2012-11-07 |
| CN102764285B CN102764285B (en) | 2014-09-24 |
Family
ID=47092012
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210270799.7A Active CN102764285B (en) | 2012-08-01 | 2012-08-01 | Coreopsis tinctoria extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102764285B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103030993A (en) * | 2012-11-10 | 2013-04-10 | 新疆维吾尔自治区中药民族药研究所 | Process method for extracting coreopsis tinctoria nutt food colorant |
| CN103222996A (en) * | 2013-05-15 | 2013-07-31 | 顾海 | Coreopsis tinctoria refined extract and medical application thereof |
| CN104839565A (en) * | 2015-06-01 | 2015-08-19 | 新疆生命核力高科股份有限公司 | Plains coreopsis noodles and processing method thereof |
| CN105504863A (en) * | 2015-11-16 | 2016-04-20 | 南京农业大学 | Method for using macroporous adsorption resin for purification of coreopsis tinctoria flower pigment |
| CN106943441A (en) * | 2017-02-22 | 2017-07-14 | 浙江大学 | Dichromatism coreopsis extract and its application in antidepressant is prepared |
| CN108783457A (en) * | 2018-06-22 | 2018-11-13 | 苏州大学 | A kind of preparation method and applications of the coreopsis tinctoria extract containing maritimetin |
| CN116172201A (en) * | 2023-03-17 | 2023-05-30 | 浙江大学 | Tagetes extract and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102266369A (en) * | 2011-05-27 | 2011-12-07 | 新疆维吾尔自治区药物研究所 | Application of calliopsis extract to liver protection and antioxidation |
-
2012
- 2012-08-01 CN CN201210270799.7A patent/CN102764285B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102266369A (en) * | 2011-05-27 | 2011-12-07 | 新疆维吾尔自治区药物研究所 | Application of calliopsis extract to liver protection and antioxidation |
Non-Patent Citations (1)
| Title |
|---|
| 曹燕等: "金鸡菊提取物体外抗氧化活性", 《中国实验方剂学杂志》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103030993A (en) * | 2012-11-10 | 2013-04-10 | 新疆维吾尔自治区中药民族药研究所 | Process method for extracting coreopsis tinctoria nutt food colorant |
| CN103222996A (en) * | 2013-05-15 | 2013-07-31 | 顾海 | Coreopsis tinctoria refined extract and medical application thereof |
| CN104839565A (en) * | 2015-06-01 | 2015-08-19 | 新疆生命核力高科股份有限公司 | Plains coreopsis noodles and processing method thereof |
| CN105504863A (en) * | 2015-11-16 | 2016-04-20 | 南京农业大学 | Method for using macroporous adsorption resin for purification of coreopsis tinctoria flower pigment |
| CN106943441A (en) * | 2017-02-22 | 2017-07-14 | 浙江大学 | Dichromatism coreopsis extract and its application in antidepressant is prepared |
| CN106943441B (en) * | 2017-02-22 | 2022-05-31 | 浙江大学 | Coreopsis tinctoria extract and application thereof in preparation of antidepressant drugs |
| CN108783457A (en) * | 2018-06-22 | 2018-11-13 | 苏州大学 | A kind of preparation method and applications of the coreopsis tinctoria extract containing maritimetin |
| CN116172201A (en) * | 2023-03-17 | 2023-05-30 | 浙江大学 | Tagetes extract and preparation method and application thereof |
| CN116172201B (en) * | 2023-03-17 | 2024-05-10 | 浙江大学 | Tagetes extract and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102764285B (en) | 2014-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102764285B (en) | Coreopsis tinctoria extract | |
| CN101391060B (en) | Preparation method of bamboo leaf flavone | |
| Wan et al. | Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures | |
| CN104492393B (en) | Flavonoid substances separation method in Camellia nitidissima Chi based on magnetic nano-particle-PAMAM nano composite material | |
| CN101955688A (en) | Method for extracting and preparing flavonoids pigment from chrysanthemum | |
| HUI et al. | PRELIMINARY PHYTOCHEMICAL SCREENING AND EFFECT OF HOT WATER EXTRACTION CONDITIONS ON PHENOLIC CONTENTS AND ANTIOXIDANT CAPACITIES OF Morinda citrifolia LEAF. | |
| CN102464685A (en) | Method of extracting flavonoid glycosides from grosvenor monordica leaves and product thereof | |
| CN102775811A (en) | Process of extracting flavonoids from Coreopsis bicolor | |
| CN104523479B (en) | Application and whitening articles for use of the himalayan prinsepia leaf extract in whitening articles for use | |
| Raaman et al. | Antioxidant and anticancer activity of Helicteres isora dried fruit solvent extracts | |
| Shailaja et al. | Evaluation of standardisation parameters, pharmacognostic study, preliminary phytochemical screening and in vitro antidiabetic activity of Emblica officinalis fruits as per WHO guidelines | |
| Sharifi-Rad et al. | Phytochemical analysis, antioxidant and antibacterial activities of various extracts from leaves and stems of Chrozaphora tinctoria | |
| Odo et al. | The antidiabetic activities of the methanolic root bark extract of Afzelia africana in alloxan-induced diabetic mice | |
| Aliyu et al. | In vitro evaluation of antioxidant activity of Anisopus mannii NE Br. | |
| CN102362876A (en) | Chinese redwood bark extract, preparation method thereof and purpose thereof | |
| WO2009107959A2 (en) | Method for separating valuable flavonoid-containing fraction and novel flavonoid substance from above-ground part of the tree named sageretia theezans | |
| Vinodhini et al. | Antioxidant activity of the isolated compounds, methanolic and hexane extracts of Toona ciliata leaves | |
| CN103071021A (en) | Method for extracting flavonoids from Allium mongolicum Regel leaves | |
| Butnariu | Plant genome engineering for improved flavonoids production | |
| Choi et al. | Antitumor activity of cell suspension culture of green tea seed (Camellia sinensis L.) | |
| Gul et al. | Preliminary phytochemical screening of Ficus religiosa for identification of bioactive compounds | |
| CN111067926B (en) | Method for extracting and enriching total flavonoids and quinic acid ester compounds in rhinacanthus nasutus | |
| KR20200096349A (en) | Composition of Anti-Oxidant Cosmetics Using Energy Saving Molecular Cluster Supercritical Centella Asiatica Extract | |
| Nazlić et al. | Free volatile compounds of Veronica austriaca ssp. jacquinii (Baumg.) Eb. Fisch. and their biological activity. Plants 2021, 10, 2529 | |
| CN118370708A (en) | Preparation method of angelica keiskei extract, product and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160113 Address after: 830011 the Xinjiang Uygur Autonomous Region high tech Zone of Urumqi city (urban) Beijing Road No. 556 diamond Baixin building 4 court A shop floor room 15 Patentee after: Xinjiang agricultural biological Polytron Technologies Inc Address before: 830000, room 4, building 1, 458, science building, No. 416-427, Northwest Road, the Xinjiang Uygur Autonomous Region, Urumqi Patentee before: Urumqi Sangaohe Pharmaceutical Co., Ltd. |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20210809 Address after: 830011 shop 14, floor 4, building a, Baixin Diamond Garden, No. 556, Beijing South Road, high tech Zone (new urban area), Urumqi, Xinjiang Uygur Autonomous Region Patentee after: Xinjiang Xueju Bio-technology Co.,Ltd. Address before: 830011 room 15, shop 4 / F, building a, Baixin Diamond Garden, No. 556, Beijing South Road, high tech Zone (new urban area), Urumqi, Xinjiang Patentee before: XINJIANG NONGDING BIOTECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right |