CN102763615A - Full-manual breeding method for megalonibea fusca - Google Patents
Full-manual breeding method for megalonibea fusca Download PDFInfo
- Publication number
- CN102763615A CN102763615A CN2012102268504A CN201210226850A CN102763615A CN 102763615 A CN102763615 A CN 102763615A CN 2012102268504 A CN2012102268504 A CN 2012102268504A CN 201210226850 A CN201210226850 A CN 201210226850A CN 102763615 A CN102763615 A CN 102763615A
- Authority
- CN
- China
- Prior art keywords
- fish
- day
- water
- days
- feeding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a full-artificial breeding method for megalonibea fusca. The method comprises the following steps of: getting parent fish seeds from natural sea areas; carrying out reinforced breeding on parent fish; carrying out artificial spawning on the parent fish; carrying out hatching on fertile eggs; carrying out indoor artificial cultivation on juvenile fish; carrying out indoor nutrition strengthening cultivation on the juvenile fish in an alevin stage; carrying out ecological breeding on the alevin which are moved into an earthen pond after the alevin stage; and breeding the alevin into large-size seedlings, and the like. The full-artificial breeding method provided by the invention is a mode applicable to the large-scale production of megalonibea fusca fries. After carrying out three-year cultivation on first-generation juvenile fish which is successfully artificially bred, sexually-matured first-filial-generation parent fish is cultivated, and then second-generation juvenile fish is bred from the first-filial-generation parent fish, thereby achieving the full-artificial breeding. The full-artificial breeding method breaks through the bottleneck that because the megalonibea fusca belongs to endangered fish species, parent fish sources are extremely difficult, and realizes the large-scale artificial breeding and culturing industrialization of the megalonibea fusca.
Description
Technical field
The invention belongs to aquaculture technology, involving artificial propagation of marine fish breeding methods, in particular relates to a brown wool Chang artificial propagation seedling method.
Background technology
Brown hair Chang (Megalonibea Fusca) is Zhu Yuanding, etc. (1963) named the new species belonging Sciaenidae (Sciaenidea), Mao Chang genus.Be the fingerling that only there is distribution in China,, mainly be distributed in the East Sea, southern Yellow Sea, the Taiwan Straits and the South Sea for the warm large-scale fish of warm nature bottom in coastal waters.Adult fish generally weighing 40-50kg, the maximum individual up to 70-100kg, its swim bladder is extremely rare fine tonic history in Zhejiang area per 1000 g brown hair Chang Maw price of 3-5 million, and spread a saying: "Wood Tong charge yellow officials", brown hair Chang fish in Wenzhou, Zhejiang Province, Zhoushan area commonly known as "wood Tong", "net Tong."" yellow official " is yellow lip fish, and a Bahaba flavolabiata (Lin) price among the people is hundreds thousand of units and even 1,000,000 yuan."Wood hit" The ability to charge "yellow officials", it shows the value of high grade, in coastal Zhejiang grade brown hair behind the value Chang bahaba has important economic value.Brown hair Chang for our country one of a handful of large fish, natural sea resources are very scarce in recent decades has almost disappeared, and is an endangered species of rare and valuable, has an important economic value and high academic value.Brown hair Chang on basic biology, reproductive biology, breeding biology, breeding biology research at home and abroad in the blank.Because brown hair Chang in Sciaenidae classified location plays an important role as well as having an important economic value.So carry brown hair Chang's artificial breeding technology research, and to achieve full artificial breeding for brown hair Chang this precious rare and endangered species artificial reproduction and recovery resources are of great significance.
Brown hair Chang rapid growth, farmed year weigh 2-3kg, two years up to 7-8kg, three years up to 12-13kg, the maximum individual up to 15kg or more.Culturing profit and can reach 66%-78%, is that seawater cage culture dealer's first-selection is cultured object, but the natural waters natural seeding, wild fry almost disappeares, and can't satisfy the active demand of aquaculture.Therefore a need for a special brown hair Chang artificial breeding methods, especially of artificial breeding methods to achieve brown hair Chang scale aquaculture seed production to meet demand.
Summary of the invention
The invention provides a brown wool Chang wholly artificial breeding methods, characterized by its technology to get the original species from natural waters broodstock, broodstock intensive training artificial spawning, hatching, larvae artificial cultivation.Artificial breeding success first from generation to generation juvenile fish cultivate sexually matured first generation filial generation parent population through the breed in 3 years, utilize first generation filial generation parent population to breed out second juvenile fish from generation to generation, realize the artificial seedling rearing.
The object of the present invention is achieved through the following technical solutions A brown hair of artificial breeding Chang method comprising the steps of:
(1) original seed parent population reinforced cultivating: the original seed parent population of introducing a fine variety places marine net cage reinforced cultivating, promotes gonadal maturation.
(2) artificial spawning broodstock: brown hair Chang in reproductive season, select gonads mature spawning females and males into the indoor pool artificial oxytocin, oxytocin injections to control spawning temperature 24.0-26.0 ℃, salinity of 29-32 ‰ so naturally fertilized eggs.Gathered up fertilized egg in second day.
(3) fertilized egg hatching: fertilized egg places hatching pail to hatch, ten thousand/m3 of control incubating oosperm density 100-150 water body, and 24.0-26.0 ℃ of hatching water temperature, salinity 29-32 ‰, treats that moving into nursery pond again before embryonic development extremely is about to hatch hatches prelarva.
(4) prelarva is cultivated: the fertilized egg immigration nursery pond that step (3) is obtained hatches prelarva, lays the continuous little inflation of gas exhausting stone with 1.5-2.0 ㎡ area.The wheel animalcule young of throwing something and feeding during the prelarva opening.1-5 age in days prelarva is cultivated density 2.0-2.5 ten thousand tails/m3; Cultivate 24.0-26.0 ℃ of water temperature, salinity 29-32 ‰, the 3-5 age in days prelarva wheel animalcule young 5-6/ml that throws something and feeds; And add chlorella 10-15 ten thousand cell/ml, add the fresh sand filtration seawater of 10-15% every day.6-18 age in days postlarva is cultivated density 0.5-1.0 ten thousand tails/m3; Cultivate 24.0-26.0 ℃ of water temperature, salinity 28-30 ‰, big a little aeration quantity; The wheel animalcule density of throwing something and feeding 7-15/ml; And add chlorella 20-25 ten thousand cell/ml, and since 13 ages in days collocation a spot of artemia nauplii of throwing something and feeding, the 13-18 age in days artemia nauplii 1.0-5.0/ml that throws something and feeds.Day change water once, day quantity of exchanged water 15-50%, every day soil pick-up once.19 age in days prelarva completion morphologies are grown and are got into the postlarva phase.
(5) postlarva is cultivated: the postlarva that step (4) is obtained continues to cultivate at nursery pond, cultivates density 2000-2500 tail/m3, cultivates 24-28 ℃ of water temperature, and salinity 25-28 ‰.Strengthen the little state that rolls of aeration quantity to the water surface.Flowing water is cultivated a day quantity of exchanged water 50-100%, every day soil pick-up once.The 19-25 age in days halogen worm 5-13/ml that throws something and feeds, 16 ages in days begin the collocation live body copepod larva 2-3/ml that throws something and feeds.About 26 ages in days get into young stage.
(6) juvenile fish is cultivated: the juvenile fish that step (5) is obtained continues to cultivate at nursery pond, cultivates density 1000-2000 tail/m3, cultivates 24-28 ℃ of water temperature, and salinity 25-28 ‰.Flowing water is cultivated a day quantity of exchanged water 100-300%, every day soil pick-up once.Live body copepoda 0.5-1.8kg throws something and feeds per ten thousand tail juvenile fish days of 26-50 age in days.Indoor culture 45-50 days, the juvenile fish body reaches the 4-5cm commercial specification can provide cage culture.Or
(7) seedling rearing in earth ponds: the 8-18 age in days postlarva that step (4) is obtained moves into sea pond to carry out young juvenile fish and cultivates, and throws in postlarva 4-5 ten thousand tails for every mu, young every day postlarva phase throw something and feed fish slurry 3 times of full pool spilling head; Day feeding volume 0.5-1.5kg/ mu, day quantity of exchanged water 20-30% is to the fish gruel 3 times of throwing something and feeding of young stage full pool spilling head every day; Day feeding volume 2.0-3.0kg/ mu, day quantity of exchanged water 30-70% cultivates 24-30 ℃ of water temperature; Salinity 20-30 ‰, PH8.0-8.5, transparency 40-50cm.Tu Chi cultivated 40-45 days, and the juvenile fish body reaches 6.0-7.0cm and can go out the pool cage culture is provided.
(8) with step (6) or (7) obtain first from generation to generation juvenile fish marine cage culture 3 years are provided, cultivate into sexually matured first generation filial generation parent population.
(9) first generation filial generation parent population that utilizes step (8) to obtain gonad maturity breeds out second juvenile fish from generation to generation, realizes the artificial seedling rearing.
The described original seed parent population of step (1) is meant that the fragmentary natural seeding, wild fry that natural waters is caught once in a while reached sexually matured parent in 3 years through cage culture.
The described marine cage size of step (1) is 6.0m * 3.0m * 3.0m, mesh 4-5cm, and parent fish rearing density is every cubic metre of water body 5-6 ㎏.
The described parent population cultivation method for enhancing of step (1) is all to throw something and feed that (here " anniversary " refers to the whole time to high-quality bait the anniversary; Except that the phase of surviving the winter); Throw something and feed every day once; The high-quality bait secondary of throwing something and feeding the October-every day in December before the parent fish live through the winter, the high-quality bait secondary of throwing something and feeding every day late Febuary in next year to late April, a day feeding rate is fish physique amount 1.5-3.5%.Parent population mid-February in late December to next year water temperature be lower than 13.0 ℃ overwintering period and stop to ingest; And being in the grand period of growth yolk that the parent population sexual gland grows the III phase from the II phase, whole overwintering period accumulates the stage; Needing body accumulation a large number of nutrients to be transformed into gonad development gets on; If obviously parent population does not carry out the fortification cultivation before surviving the winter, the overwintering period gonad development is with regard to nonnutritive source, and gonad development will be affected.The quality of the food is fresh or chilled round scad, anchovies , Ammodytes other trash.
Step (2) brown hair Chang described the reproductive season, from April to June for the annual spring and autumn from September to November, spawning temperature 23.0-26.0 ℃, spawning peak of May and October.Study found that brown hair Chang sexual cycle of one year has two reproductive seasons.The raun of the described gonad maturity of step (2) is that belly expands softness; Egg cell reaches 500-600 μ m; Gonad development has arrived female parent of the IV end of term, and described gonad maturity milter is that belly expands softness, the male parent of gently pressing belly promptly to have full seminal fluid to flow out.
The described spawning pond area of step (2) 30-35 ㎡, depth of water 1.5-1.8 rice.
The described induced spawning method of step (2) is a parent population after the anesthesia of the 25ppm eugenol aqueous solution, thoracic cavity injection luteinizing hormone releasing hormone 4.5 ㎎+human chorionic gonadtropin 250 μ g/kg fish physique amounts, the same dosage of male and female.Raun: the milter ratio is 1:1.2-1.3,, the back parent population of hastening parturition was put into the spawning pond stimulation by running water 4-6 hour, made its natural mating spawning and fertilization.24.0-26.0 ℃ of water temperature of control, salinity 29-32 ‰, and parent population effect time of laying eggs is 30-36 hour.
The described method for incubating oosperm of step (3) is that the ovum that spawning pond is collected is focused in the big basin of the seawater that fills salinity 28-33 ‰, precipitates and isolates the fertilized egg of come-up, hatches in the conical bottom fiberglass hatching pail of immigration volume 0.5m3 after rinsing well.Ten thousand/m3 of control incubating oosperm density 100-150 water body, water temperature 24.0-26.0 ℃, salinity 29-32 ‰, and day is changed the water secondary, day quantity of exchanged water 150%, continuous little inflation.Treating that embryonic development to heart beat period is moved into again hatches prelarva in the nursery pond.
The method that the said fertilized egg of step (3) places hatching pail to hatch helps precipitating and isolating once more the dead embryo of hatching process, avoids the dead corrupt water quality that worsens of embryo of the direct lower storage reservoir hatching of fertilized egg.Hatching pail hatching fertilized egg helps the regulation and control of water temperature, salinity, water quality, saves water resource and electric energy.
The described nursery pond of step (4) is the square cement pit of interior length, area 20-25 ㎡, depth of water 1.5-1.6 rice.
The described prelarva opening time of step (4) is for hatching back 48 hours.The long 140-160 μ of said wheel animalcule young body m.
The described wheel animalcule of step (4) is brachionus plicatilis (Brachionus plicatilis).
Before throwing something and feeding, must strengthen 12 hours the said brachionus plicatilis of step (4) through 50DE wheel animalcule nutrition fortifier 15ppm and the chlorella more than density 2,000 ten thousand cell/ml; Every cubic metre of water body is thrown in hundred million of wheel animalcule 3-5; Add 50DE-micro-capsule 15ml, inflation was strengthened 12 hours.With the nutriture value that the improves wheel animalcule content of DHA and EPA particularly, satisfy prelarva to n-3 must fatty acid nutritional need, raising prelarva survival rate.
The said chlorella of step (4) is at least a in chlorella vulgaris (Chlorella vulgaris) or the chlorella pyrenoidosa (Chlorella pyrenoidosa).
The said interpolation of step (4) chlorella can be improved nursery pond water quality, and is that wheel animalcule provides bait in the nursery pond.
The described halogen worm of step (4) is at least a in Arthropoda, Anostraca, the genus artemia (Artemia sinica).
Before throwing something and feeding, the said halogen worm of step (4) must strengthen 6 hours through 50DE halogen worm nutrition fortifier 30ppm; Every cubic metre of water body is thrown in hundred million of halogen worm 1-2; Add 50DE-micro-capsule 30ml, inflation was strengthened 6 hours, with the nutriture value that improves the halogen worm content of DHA and EPA particularly; Satisfy the nutritional need of postlarva, improve young juvenile fish survival rate the necessary fatty acid of n-3.
Step (4) said 50DE wheel animalcule nutrition fortifier and 50DE halogen worm nutrition fortifier are that Shandong rises the rope fishing with feed research center product.
DHA Han Liang ≧ 50% of the said 50D-micro-capsule of step (4) (M type).Wheel animalcule, artemia larvae DHA content after the 50DE micro-capsule is strengthened improves 480% and thousands of times respectively.Survival rate, DHA content, the vigor of the young postlarva of wheel animalcule after the reinforcement, artemia nauplii feeding marine improve 30%, 155% respectively, more than 50%.
The said copepoda of step (5) is that bidentate is permitted water flea (Schmackeria dubia), ham and permitted at least a in water flea (Schmackeria poplesia), Calanus sinicus (Calanus sinicus), exquisite magnificent wise water flea (Sinocalanus tenellus), the Ke Shi spindle water flea (Acartia clausi).
The copepoda of the said young juvenile fish of throwing something and feeding of step (5) must be a live body, to guarantee the good water quality of nursery pond.Described copepoda contains the necessary fatty acid of abundant n-3, can satisfy the nutritional need of young juvenile fish to necessary fatty acid.
It is complete that the characteristic of the said juvenile fish of step (6) is that fish body whole body squama drapes over one's shoulders, complete the completions metamorphosis of the whole allelotaxises of fry this moment.Described indoor larvae juvenile fish was cultivated about 50 days time, and the juvenile fish body reaches the 4-5cm commercial specification can provide breed.
The said seedling rearing in earth ponds method of step (7) is: select freely to receive tide, convenient, the silt substrate of draining; Area 10-20 mu, depth of water 1.5-2.0 rice, the sea pond of salinity 20-30 ‰, before putting fry 8-10 days; Add quicklime 20kg with bleaching powder 10kg and convert the sterilization of water full pool spilling head; Inject the fresh seawater of 50-60cm after three days, water inlet is established the block filtration that 60 mesh sieve thin,tough silk are processed, and prevents that the trash fish shrimp from getting in the pond.Rub the adult fish slurry with trash fish through meat grinder every day and convert the water full pool spilling head, rich water is cultivated food organisms, in the 4-5 days after-bays food organisms particularly a large amount of breedings of copepod larva can throw in postlarva.
The said Tu Chi of step (7) water colour that fish slurry rich water cultivates of splashing is green clearly, promptly is main green algae with the chlorella, and this kind water stabilization has the effect of improving water quality and ecotope.The food organisms of breeding mainly contain wheel animalcule, cladocera, the copepoda of zooplankton through the eutrophic water pond, and wherein normal biomass with copepoda has comparative advantage.Tu Chi cultivates the young postlarva copepoda in the pond that mainly ingests in early stage, and along with the corresponding minimizing of copepoda quantity in increase and the pond of ingesting of young juvenile fish, later stage juvenile fish feeding habits convert the fish gruel of mainly ingesting into.Tu Chi cultivated 40-45 days, and body reaches the 6-7cm commercial specification and can go out the pool breed is provided.
The described marine cage culture method of step (8) is: require water temperature range at 13-31 ℃, salinity 20-30 ‰.The long 5-10cm juvenile fish of body breeding density is 40-50 tail/m3 water body, and the bait of throwing something and feeding is that fish is rotten, day bait throwing in secondary, and daily ration, feeding quantity is fish physique amount 10-15%; The long 20-30cm fingerling stocking of body density is 20-25 tail/m3 water body; The bait of throwing something and feeding is the trash fish piece that minces, day bait throwing in secondary, and daily ration, feeding quantity is fish physique amount 8-10%; Individual 2-3kg cultivation density is 4-5 tail/m3 water body, day bait throwing in once, feeding rate is 3-4%; The bait of throwing something and feeding is fresh or ices bright small fish, and individual 4-5kg cultivation density is 2-2.5 tail/m3 water body, and day bait throwing in once; Feeding rate is 3-4%, and the bait of throwing something and feeding is fresh or ices bright small fish; Individual 6-13kg cultivation density is 1-2 tail/m3 water body, day bait throwing in once, feeding rate is 2-3%, the bait of throwing something and feeding is fresh or ices bright small fish.Cultured body weight through 3 years and can reach 12-13kg, property is ripe.
Step (8) brown hair Chang said sexually mature male and female individuals ages of three age.
The described first generation filial generation parent population that utilizes step (8) to obtain of step (9); Raun and the milter of picking out gonadal maturation at breeding season move into indoor spawning pond and carry out artificial induced spawning; Second juvenile fish is from generation to generation cultivated in repeating step (2), (3), (4), (7), promptly realizes the artificial seedling rearing.
The present invention is relative, and background technology has the following advantages:
1 to provide a brown wool Chang artificial breeding methods, especially brown hair Chang wholly artificial breeding methods, addressing natural sea brown hair Chang resources are very scarce, broodstock source extremely difficult bottleneck, so that massive brown hair Chang artificial breeding, seed production can be achieved, meet the needs of aquaculture farming.
2, the present invention provides a kind of alevin stage of indoor fortification to cultivate, and grows seedlings with later stage immigration earth pond ecological and cultivates the seed production model that big specification seedling combines.The pond ecological breeding has the living environment requirement that natural ecological condition meets son, young, juvenile fish; Natural food organisms kind is abundant, and comprehensive nutrition is balanced, and size adapts to the selectivity of each developmental stage larvae juvenile fish to the agreeable to the taste bait of ingesting; The fry fast growth; The seedling body is healthy and strong, is convenient to characteristics such as large-scale production large size seedling seed, is the method for optimizing that seed scale, industrialization are produced.
Embodiment
Below in conjunction with specific embodiment the present invention is further described, but embodiment of the present invention is not limited thereto.
Embodiment 1,1998 implements the place and advances flourish aquatic products development corporation, Ltd. nursery at Ningde City.
(1) original seed parent population reinforced cultivating: the original seed parent population of introducing a fine variety places marine net cage reinforced cultivating, promotes gonadal maturation.
(2) artificial spawning broodstock: brown hair Chang in the reproductive season from April to June, select abdominal swelling soft, egg reaches 500-600μm, Ⅳ gonadal development has come to the end of the female, and abdominal swelling soft, gently press the abdomen that is full of semen outflow male, spawning pool into an indoor artificial oxytocin.Parent population body weight 7.5-18kg, after the anesthesia of the 25ppm eugenol aqueous solution, thoracic cavity injection luteinizing hormone releasing hormone 4.5 ㎎+human chorionic gonadtropin 250 μ g/kg fish physique amounts, the same dosage of male and female.Raun: the milter ratio is 1:1.2-1.3, and the back parent population of hastening parturition was put into the spawning pond stimulation by running water 4-6 hour, made its natural mating spawning and fertilization, spawning pond area 36 ㎡.From hastening parturition altogether the 4-6 month 6 batches, raun 16 tails of hastening parturition, milter 21 tails, parent population spawning rate 93.7%, fertilization rate 66.6%-95.2% obtains 2,280 ten thousand in fertilized egg.Parent population lays eggs water temperature 23.0-26.0 ℃, and salinity 28-29 ‰, and the effect of the laying eggs time is 30-36 hour.
(3) fertilized egg hatching: parent population lays eggs and spawning pond fertilized egg was collected in back second day, focuses in the big basin that fills salinity 29 ‰ sand filtration seawater, precipitates and isolates the fertilized egg of come-up; Place the continuous little inflation of hatching pail hatching; Day is changed the water secondary, day quantity of exchanged water 150%, ten thousand/m3 of incubating oosperm density 100-150 water body; 23.0-26.0 ℃ of hatching water temperature, salinity 28-29 ‰.Treat that embryonic development to heart beat period moves into nursery pond again and continues hatching, fertilized egg is hatched 22-26 hour prelarva time.
(4) prelarva is cultivated: the fertilized egg immigration nursery pond that step (3) is obtained hatches prelarva.Nursery pond specification 6.0m * 4.0m * 1.5m lays the continuous little inflation of gas exhausting stone with 1.5-2.0 ㎡ area.Hatch 48 hours openings of back prelarva, strengthened 12 hours through 50DE wheel animalcule nutrition fortifier 15ppm and the inflation of the chlorella more than density 2,000 ten thousand cell/ml before the wheel animalcule young of throwing something and feeding during the prelarva opening, wheel animalcule are thrown something and fed.1-5 age in days prelarva is cultivated density 2.0-2.5 ten thousand tails/m3; Cultivate 24.0-26.0 ℃ of water temperature, salinity 28-29 ‰, the 3-5 age in days prelarva wheel animalcule young 5-6/ml that throws something and feeds; And add chlorella 10-15 ten thousand cell/ml, add the fresh sand filtration seawater of 10-15% every day.6-18 age in days postlarva is cultivated density 0.5-1.0 ten thousand tails/m3; Cultivate 24.0-26.0 ℃ of water temperature, salinity 28-29 ‰, big a little aeration quantity; The wheel animalcule density of throwing something and feeding 7-15/ml; And add chlorella 20-25 ten thousand cell/ml, and since 13 ages in days collocation a spot of artemia nauplii of throwing something and feeding, the 13-18 age in days artemia nauplii 1.0-5.0/ml that throws something and feeds.Before throwing something and feeding, the halogen worm strengthened 6 hours through the inflation of 50DE halogen worm nutrition fortifier.Day change water once, day quantity of exchanged water 15-50%, every day soil pick-up once.19 age in days prelarva completion morphologies are grown and are got into the postlarva phase.
(5) postlarva is cultivated: the postlarva that step (4) is obtained continues to cultivate at nursery pond, cultivates density 2000-2500 tail/m3 water body, cultivates 24-28 ℃ of water temperature, and salinity 23-28 ‰.Strengthen the little state that rolls of aeration quantity to the water surface.Flowing water is cultivated a day quantity of exchanged water 50-100%, every day soil pick-up once.The 19-25 age in days halogen worm 5-13/ml that throws something and feeds strengthened 6 hours through the inflation of 50DE halogen worm nutrition fortifier before the halogen worm is thrown something and fed.16 ages in days begin the live body copepod larva 2-3/ml that throws something and feeds that arranges in pairs or groups.About 26 ages in days get into young stage.
(6) juvenile fish is cultivated: the juvenile fish that step (5) is obtained continues to cultivate at nursery pond, cultivates density 1000-2000 tail/m3 water body, cultivates 26-30 ℃ of water temperature, and salinity 18-28 ‰.Flowing water is cultivated a day quantity of exchanged water 100-300%, every day soil pick-up once.Live body copepoda 0.5-1.5kg throws something and feeds per ten thousand tail juvenile fish days of 26-40 age in days.Indoor culture overall process 31-41 days is cultivated abnormal juvenile fish 66.6 ten thousand tails, and the juvenile fish total length reaches 31-49mm, and survival rate of seedling reaches 25.3%.Move into marine net cage and carry out intermediate rearing.
Embodiment 2, calendar year 2001 implement the place in the Zhaoan County marine fishery nursery.
(1) parent fish rearing: with bred first in 1998 success first from generation to generation juvenile fish marine cage culture is provided, cultivate through 3 years, calendar year 2001 obtains into sexually matured first generation filial generation parent population.
(2) artificial spawning broodstock: 2001 brown hair Chang in the reproductive season from April to June and from September to November, select abdominal swelling soft, egg reaches 500-600μm, Ⅳ gonadal development has come to the end of the female, and abdominal swelling soft, Gently press the abdomen that is full of semen of the male, spawning pool into an indoor artificial oxytocin.Parent population is after the anesthesia of the 25ppm eugenol aqueous solution, and luteinizing hormone releasing hormone 4.5 ㎎+human chorionic gonadtropin 250 μ g/kg fish physique amounts, the same dosage of male and female are injected in the thoracic cavity.Raun: the milter ratio is 1:1.2-1.3, and the back parent population of hastening parturition was put into the spawning pond stimulation by running water 4-6 hour, made its natural mating spawning and fertilization, and parent population lays eggs water temperature 23.5-26.5 ℃, and salinity 31-32 ‰.
(3) fertilized egg hatching: parent population lays eggs and spawning pond fertilized egg was collected in back second day, focuses in the big basin that fills salinity 32 ‰ sand filtration seawater, precipitates and isolates the fertilized egg of come-up; Place the continuous little inflation of hatching pail hatching; Day is changed the water secondary, day quantity of exchanged water 150%, ten thousand/m3 of incubating oosperm density 100-150 water body; 24.0-26.5 ℃ of hatching water temperature, salinity 31-32 ‰.Treat that embryonic development to heart beat period moves into nursery pond again and continues hatching.
(4) prelarva is cultivated: the fertilized egg immigration nursery pond that step (3) is obtained hatches prelarva.Nursery pond specification 6.0m * 4.0m * 1.8m lays the continuous little inflation of gas exhausting stone with 1.5-2.0 ㎡ area.Hatch 48 hours openings of back prelarva, strengthened 12 hours through 50DE wheel animalcule nutrition fortifier 15ppm and the inflation of the chlorella more than density 2,000 ten thousand cell/ml before the wheel animalcule young of throwing something and feeding during the prelarva opening, wheel animalcule are thrown something and fed.1-5 age in days prelarva is cultivated density 2.0-2.5 ten thousand tails/m3; Cultivate 24.0-26.5 ℃ of water temperature, salinity 31-32 ‰, the 3-5 age in days prelarva wheel animalcule young 5-6/ml that throws something and feeds; And add chlorella 10-15 ten thousand cell/ml, add the fresh sand filtration seawater of 10-15% every day.6-18 age in days postlarva is cultivated density 0.5-1.0 ten thousand tails/m3; Cultivate 24.0-26.5 ℃ of water temperature, salinity 28-29 ‰, big a little aeration quantity; The wheel animalcule density of throwing something and feeding 7-15/ml; And add chlorella 20-25 ten thousand cell/ml, and since 13 ages in days collocation a spot of artemia nauplii of throwing something and feeding, the 13-18 age in days artemia nauplii 1.0-5.0/ml that throws something and feeds.Before throwing something and feeding, the halogen worm strengthened 6 hours through the inflation of 50DE halogen worm nutrition fortifier.Day change water once, day quantity of exchanged water 15-50%, every day soil pick-up once.19 age in days prelarva completion morphologies are grown and are got into the postlarva phase.
(5) seedling rearing in earth ponds: the 10-18 age in days postlarva immigration sea pond that step (4) obtains is carried out young juvenile fish cultivation.Select freely to receive tide, convenient, the silt substrate of draining; Area 10-20 mu, depth of water 1.5-2.0 rice, the sea pond of salinity 20-25 ‰, before putting fry 8-10 days; Add quicklime 20kg with bleaching powder 10kg and convert the sterilization of water full pool spilling head; Inject the fresh seawater of 50-60cm after three days, water inlet is established the block filtration that 60 mesh sieve thin,tough silk are processed, and prevents that the trash fish shrimp from getting in the pond.Rub the adult fish slurry with trash fish through meat grinder every day and convert the water full pool spilling head, rich water is cultivated food organisms, in the 4-5 days after-bays food organisms particularly a large amount of breedings of copepod larva can throw in postlarva.Throw in postlarva 4-5 ten thousand tails for every mu, young every day postlarva phase throw something and feed fish slurry 3 times of full pool spilling head, day feeding volume 0.5-1.5kg/ mu; Day quantity of exchanged water 20-30% is to young stage full pool spilling head every day rotten 3 times of the fish of throwing something and feeding, day feeding volume 2.0-3.0kg/ mu; Day quantity of exchanged water 30-70%, 18.4-31.5 ℃ of cultivating process water temperature, salinity 13-25 ‰; PH8.3-8.6, transparency 40-50cm.The Tu Chi water colour that fish slurry rich water cultivates of splashing is green clearly, promptly is main green algae with the chlorella, and this kind water stabilization has the effect of improving water quality and ecotope.The food organisms of breeding mainly contain wheel animalcule, cladocera, the copepoda of zooplankton through the eutrophic water pond, and wherein normal biomass with copepoda has comparative advantage.Tu Chi cultivates the young postlarva copepoda in the pond that mainly ingests in early stage, and along with the corresponding minimizing of copepoda quantity in increase and the pond of ingesting of young juvenile fish, later stage juvenile fish feeding habits convert the fish gruel of mainly ingesting into.Tu Chi cultivated 37-50 days, and the juvenile fish average body is long to reach the 4.8-8.6cm commercial specification respectively, and can go out the pool provides breed.Realized the artificial seedling rearing.
Claims (9)
1 A brown wool Chang wholly artificial breeding method, which comprises the following steps:
(1) original seed parent population reinforced cultivating: the original seed parent population of introducing a fine variety places marine net cage reinforced cultivating, promotes gonadal maturation;
(2) artificial spawning broodstock: brown hair Chang in reproductive season, select gonads mature spawning females and males into the indoor pool artificial oxytocin, oxytocin injections to control spawning temperature 24.0-26.0 ℃, salinity of 29-32 ‰ so naturally fertilized eggs, fertilized eggs from the next collection;
(3) fertilized egg hatching: fertilized egg places hatching pail to hatch, ten thousand/m of control incubating oosperm density 100-150
3Water body, 24.0-26.0 ℃ of hatching water temperature, salinity 29-32 ‰, treats that moving into nursery pond again before embryonic development extremely is about to hatch hatches prelarva;
(4) prelarva is cultivated: the fertilized egg immigration nursery pond that step (3) is obtained hatches prelarva, lays the continuous little inflation of gas exhausting stone, the wheel animalcule young of throwing something and feeding during the prelarva opening with 1.5-2.0 ㎡ area; 1-5 age in days prelarva is cultivated density 2.0-2.5 ten thousand tails/m
3, cultivating 24.0-26.0 ℃ of water temperature, salinity 29-32 ‰, the 3-5 age in days prelarva wheel animalcule young 5-6/ml that throws something and feeds, and add chlorella 10-15 ten thousand cell/ml, add the fresh sand filtration seawater of 10-15% every day; 6-18 age in days postlarva is cultivated density 0.5-1.0 ten thousand tails/m
3, cultivating 24.0-26.0 ℃ of water temperature, salinity 28-30 ‰; Strengthen aeration quantity, the wheel animalcule density of throwing something and feeding 7-15/ml, and add chlorella 20-25 ten thousand cell/ml; Since 13 ages in days collocation a spot of artemia nauplii of throwing something and feeding, the 13-18 age in days artemia nauplii 1.0-5.0/ml that throws something and feeds; Day change water once, day quantity of exchanged water 15-50%, every day soil pick-up once, 19 age in days prelarva completion morphologies are grown and are got into the postlarva phase;
(5) postlarva is cultivated: the postlarva that step (4) is obtained continues to cultivate at nursery pond, cultivates density 2000-2500 tail/m
3, cultivating 24-28 ℃ of water temperature, salinity 25-28 ‰; Strengthen the little state that rolls of aeration quantity to the water surface, flowing water is cultivated a day quantity of exchanged water 50-100%, every day soil pick-up once; The 19-25 age in days halogen worm 5-13/ml that throws something and feeds, 16 ages in days begin the collocation live body copepod larva 2-3/ml that throws something and feeds, and about 26 ages in days get into young stage;
(6) juvenile fish is cultivated: the juvenile fish that step (5) is obtained continues to cultivate at nursery pond, cultivates density 1000-2000 tail/m
3, cultivating 24-28 ℃ of water temperature, salinity 25-28 ‰; Flowing water is cultivated a day quantity of exchanged water 100-300%, every day soil pick-up once; Live body copepoda 0.5-1.8kg throws something and feeds per ten thousand tail juvenile fish days of 26-50 age in days; Indoor culture 45-50 days, the juvenile fish body reaches the 4-5cm commercial specification can provide cage culture; Or
(7) seedling rearing in earth ponds: the 8-18 age in days postlarva that step (4) is obtained moves into sea pond to carry out young juvenile fish and cultivates, and throws in postlarva 4-5 ten thousand tails for every mu, young every day postlarva phase throw something and feed fish slurry 3 times of full pool spilling head; Day feeding volume 0.5-1.5kg/ mu, day quantity of exchanged water 20-30% is to the fish gruel 3 times of throwing something and feeding of young stage full pool spilling head every day; Day feeding volume 2.0-3.0kg/ mu, day quantity of exchanged water 30-70% cultivates 24-30 ℃ of water temperature; Salinity 20-30 ‰, PH8.0-8.5, transparency 40-50cm; Tu Chi cultivated 40-45 days, and the juvenile fish body reaches 6.0-7.0cm and can go out the pool cage culture is provided;
(8) with step (6) or (7) obtain first from generation to generation juvenile fish marine cage culture 3 years are provided, cultivate into sexually matured first generation filial generation parent population;
(9) first generation filial generation parent population that utilizes step (8) to obtain gonad maturity breeds out second juvenile fish from generation to generation, realizes the artificial seedling rearing.
(2) according to claim 1, wherein a brown wool Chang wholly artificial breeding methods, wherein: step (a) to strengthen the broodstock cultivation methods as the anniversary should be fed high-quality food, fed once a day, winter broodstock -12 month before October fed high-quality food twice a day the following year, in late February to late April-quality food twice daily feeding, feeding on fish quality was 1.5-3.5%.
3 as claimed in claim 1, wherein a total artificial breeding Chang brown hair, characterized in that: in step (2) The artificial oxytocin method: 25ppm broodstock eugenol solution by anesthesia, injection of luteinizing hormone-releasing hormone chest 4.5 mg + chorionic gonadotropin 250μg/kg quality fish, male and female same dose; female: male ratio of 1:1.2-1.3, after spawning broodstock spawning pond water into the stimulation 4-6 hours, so that the natural mating spawning fertilization; controlling water temperature 24.0-26.0 ℃, salinity of 29-32 ‰.
4 as claimed in claim 1, wherein a total artificial breeding Chang brown hair, characterized in that: in step (3) incubation of the fertilized egg is collected from the eggs of spawning tank containing concentrated salinity 28 - 33 ‰ seawater big tub, precipitation isolated floating eggs, rinse into a conical volume of 0.5m3 bucket bottom fiberglass hatch hatching; controlled hatching density 100-150 ten thousand / m3 of water, the water temperature 24.0 -26.0 ℃, salinity of 29-32 ‰, day changing the water twice a day for water 150% continuous micro-inflated, to be the heartbeat of the embryo to the pool and then moved into the nursery hatched larvae.
5 according to claim 1, wherein a brown wool Chang wholly artificial breeding methods, wherein: step (4) of the rotifer fed rotifers ago by 50DE nutrition enhancer 15ppm and density 20 million cell / ml or more of chlorella enhanced 12 hours per cubic meter of water running rotifers 3-5 billion, adding 50DE-microcapsules 15ml, inflatable strengthen 12 hours.
6 of claim 1, wherein a brown wool Chang wholly artificial breeding methods, wherein: step (4) of the brine shrimp Artemia fed ago by 50DE strengthen nutrition fortifier 30ppm 6 hours per cubic meter of water running Artemia 1-2 billion, adding 50DE-microcapsules 30ml, inflatable strengthen six hours.
7 according to claim 1, wherein a brown wool Chang wholly artificial breeding methods, wherein: step (7 ) is the Earth Ponds: Select tidal flow freely, to facilitate drainage, silt sediment, an area of 10 - 20 acres, the depth 1.5-2.0 m, salinity 20-30 ‰ seawater ponds, put the fish in the 8-10 days before, the entire pool disinfection, three days after the injection of 50-60cm of fresh seawater intake system located 60 mesh silk into blocking filter to prevent miscellaneous fish into the pool; trash after a day with minced meat grinder into a fish paste watered Quanchiposa fertilizer food organisms cultured pool after 4-5 days of food organisms in particular a large number of copepod larvae you can put in the late larval breeding.
As claimed in claim 1, wherein a brown wool Chang wholly artificial breeding methods, wherein: step (8) The sea cages farming methods are: Requirements temperature range 13-31 ℃, salinity 20-30 ‰.The long 5-10cm juvenile fish of body breeding density is 40-50 tail/m3 water body, and the bait of throwing something and feeding is that fish is rotten, day bait throwing in secondary, and daily ration, feeding quantity is fish physique amount 10-15%; The long 20-30cm fingerling stocking of body density is 20-25 tail/m3 water body; The bait of throwing something and feeding is the trash fish piece that minces, day bait throwing in secondary, and daily ration, feeding quantity is fish physique amount 8-10%; Individual 2-3kg cultivation density is 4-5 tail/m3 water body, day bait throwing in once, feeding rate is 3-4%; The bait of throwing something and feeding is fresh or ices bright small fish, and individual 4-5kg cultivation density is 2-2.5 tail/m3 water body, and day bait throwing in once; Feeding rate is 3-4%, and the bait of throwing something and feeding is fresh or ices bright small fish; Individual 6-13kg cultivation density is 1-2 tail/m3 water body, day bait throwing in once, feeding rate is 2-3%, the bait of throwing something and feeding is fresh or ices bright small fish; Culture to sexual maturity.
(10) as claimed in claim 1, wherein a total artificial breeding Chang brown hair, characterized in that: in step (9) using the procedure described in (8) obtained in the first generation offspring broodstock selected in the breeding season gonadal mature females and males moved indoors spawning pools artificial oxytocin, repeat steps (2), (3), (4), (7) a second generation bred juveniles, namely, the achievement of artificial breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102268504A CN102763615B (en) | 2012-06-29 | 2012-06-29 | Full-manual breeding method for megalonibea fusca |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102268504A CN102763615B (en) | 2012-06-29 | 2012-06-29 | Full-manual breeding method for megalonibea fusca |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102763615A true CN102763615A (en) | 2012-11-07 |
| CN102763615B CN102763615B (en) | 2013-09-04 |
Family
ID=47091382
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102268504A Expired - Fee Related CN102763615B (en) | 2012-06-29 | 2012-06-29 | Full-manual breeding method for megalonibea fusca |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102763615B (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168730A (en) * | 2013-03-19 | 2013-06-26 | 大连海洋大学 | Pacific codfish artificial hatching method |
| CN106135097A (en) * | 2016-07-05 | 2016-11-23 | 临高海丰养殖发展有限公司 | A kind of gold Stromateoides argenteus staged big water body fry rearing method |
| CN107155956A (en) * | 2017-01-07 | 2017-09-15 | 李育培 | A kind of artificial seed's breeding method of rose Du rockfish |
| CN110432190A (en) * | 2019-07-26 | 2019-11-12 | 浙江省农业科学院 | A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method |
| CN111183931A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for grouper |
| CN111194706A (en) * | 2020-02-26 | 2020-05-26 | 海南晨海水产有限公司 | Efficient artificial breeding method for Lutjanus argentimaculatus in outdoor ecological pond |
| CN111194707A (en) * | 2020-02-27 | 2020-05-26 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for red sea perch |
| CN111727912A (en) * | 2020-06-17 | 2020-10-02 | 浙江省海洋水产养殖研究所 | Striped mullet seawater pond circulating water seedling raising method |
| CN111771772A (en) * | 2020-07-23 | 2020-10-16 | 福建省水产研究所（福建水产病害防治中心） | Grouper fry breeding method |
| CN111919801A (en) * | 2020-05-22 | 2020-11-13 | 福建省水产研究所 | Method for out-of-season seed production and cultivation of south-shifted apostichopus japonicus |
| CN113711953A (en) * | 2021-08-23 | 2021-11-30 | 福建省水产研究所（福建水产病害防治中心） | Propagation and seedling raising method for hippocampus blossoming |
| CN114375879A (en) * | 2021-12-29 | 2022-04-22 | 广东省鳇鲸海洋生物科技有限公司 | Breeding method for promoting gonad development of parent fish of yellow-lipped |
| CN114651751A (en) * | 2021-12-29 | 2022-06-24 | 广东省鳇鲸海洋生物科技有限公司 | Artificial fry breeding method for yellow lip fish |
| CN114916474A (en) * | 2022-05-24 | 2022-08-19 | 昆明市水产科学研究所 | Artificial breeding method for Pseudobagrus Zhongguchi |
| CN117598228A (en) * | 2023-12-28 | 2024-02-27 | 中国水产科学研究院黄海水产研究所 | Industrial cultivation method for micropterus salmoides fries |
-
2012
- 2012-06-29 CN CN2012102268504A patent/CN102763615B/en not_active Expired - Fee Related
Non-Patent Citations (10)
| Title |
|---|
| 《Japanese Journal of Ichthyology》 19690915 Nobuhiko Taniguchi Comparative osteology of the sciaenid fishes from Japan and its adjacent waters-I.Neurocranium 第55-67页 1-9 第16卷, 第2期 * |
| HONG WAN-SHU等: "Artificial propagation and breeding of marine fish in China", 《CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY》 * |
| NOBUHIKO TANIGUCHI: "Comparative osteology of the sciaenid fishes from Japan and its adjacent waters—I.Neurocranium", 《JAPANESE JOURNAL OF ICHTHYOLOGY》 * |
| NOBUHIKO TANIGUCHI: "Comparative osteology of the sciaenid fishes from Japan and its adjacent waters-III.Premaxillary and dentary", 《JAPANESE JOURNAL OF ICHTHYOLOGY》 * |
| WANSHU HONG等: "Review of captive bred species and fry production of marine fish in China", 《AQUACULTURE》 * |
| 孙庆海等: "褐毛鲿仔、稚、幼鱼形态特征及其生态习性的初步观察", 《浙江海洋学院学报（自然科学版）》 * |
| 孙庆海等: "褐毛鲿鱼(Megalanibea fusca Chu，Lo et Wu)工厂化育苗(Industrial Seed Culture)技术的研究", 《现代渔业信息》 * |
| 尤颖哲: "褐毛鳞人工繁育技术", 《渔业现代化》 * |
| 王波等: "褐毛鲿(黄金鮸) 的形态特征及养殖试验", 《齐鲁渔业》 * |
| 胡石柳: "娩状黄姑鱼人工繁殖与育苗技术的研究", 《集美大学学报(自然科学版)》 * |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168730B (en) * | 2013-03-19 | 2014-12-10 | 大连海洋大学 | Pacific codfish artificial hatching method |
| CN103168730A (en) * | 2013-03-19 | 2013-06-26 | 大连海洋大学 | Pacific codfish artificial hatching method |
| CN106135097A (en) * | 2016-07-05 | 2016-11-23 | 临高海丰养殖发展有限公司 | A kind of gold Stromateoides argenteus staged big water body fry rearing method |
| CN107155956B (en) * | 2017-01-07 | 2020-06-12 | 李育培 | Sebastes roseus artificial fry cultivation method |
| CN107155956A (en) * | 2017-01-07 | 2017-09-15 | 李育培 | A kind of artificial seed's breeding method of rose Du rockfish |
| CN110432190A (en) * | 2019-07-26 | 2019-11-12 | 浙江省农业科学院 | A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method |
| CN111183931A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for grouper |
| CN111194706A (en) * | 2020-02-26 | 2020-05-26 | 海南晨海水产有限公司 | Efficient artificial breeding method for Lutjanus argentimaculatus in outdoor ecological pond |
| CN111194706B (en) * | 2020-02-26 | 2022-02-18 | 海南晨海水产有限公司 | Efficient artificial breeding method for Lutjanus argentimaculatus in outdoor ecological pond |
| CN111194707A (en) * | 2020-02-27 | 2020-05-26 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for red sea perch |
| CN111194707B (en) * | 2020-02-27 | 2022-03-22 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for red sea perch |
| CN111919801A (en) * | 2020-05-22 | 2020-11-13 | 福建省水产研究所 | Method for out-of-season seed production and cultivation of south-shifted apostichopus japonicus |
| CN111727912A (en) * | 2020-06-17 | 2020-10-02 | 浙江省海洋水产养殖研究所 | Striped mullet seawater pond circulating water seedling raising method |
| CN111771772A (en) * | 2020-07-23 | 2020-10-16 | 福建省水产研究所（福建水产病害防治中心） | Grouper fry breeding method |
| CN113711953A (en) * | 2021-08-23 | 2021-11-30 | 福建省水产研究所（福建水产病害防治中心） | Propagation and seedling raising method for hippocampus blossoming |
| CN114375879A (en) * | 2021-12-29 | 2022-04-22 | 广东省鳇鲸海洋生物科技有限公司 | Breeding method for promoting gonad development of parent fish of yellow-lipped |
| CN114651751A (en) * | 2021-12-29 | 2022-06-24 | 广东省鳇鲸海洋生物科技有限公司 | Artificial fry breeding method for yellow lip fish |
| CN114375879B (en) * | 2021-12-29 | 2023-07-18 | 广东省鳇鲸海洋生物科技有限公司 | Cultivation method for promoting gonad development of parent yellow lip fish |
| CN114651751B (en) * | 2021-12-29 | 2024-02-06 | 广东省鳇鲸海洋生物科技有限公司 | Artificial breeding method for yellow lip fish |
| CN114916474A (en) * | 2022-05-24 | 2022-08-19 | 昆明市水产科学研究所 | Artificial breeding method for Pseudobagrus Zhongguchi |
| CN117598228A (en) * | 2023-12-28 | 2024-02-27 | 中国水产科学研究院黄海水产研究所 | Industrial cultivation method for micropterus salmoides fries |
| CN117598228B (en) * | 2023-12-28 | 2024-06-14 | 中国水产科学研究院黄海水产研究所 | Industrial cultivation method for micropterus salmoides fries |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102763615B (en) | 2013-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102763615B (en) | Full-manual breeding method for megalonibea fusca | |
| CN101692791B (en) | Totally artificial breeding method for Coilia ectenes | |
| CN101940184B (en) | Breeding and culturing method of improved crucian carp | |
| CN102124974B (en) | Method for artificially breeding seed of spotted maigre | |
| CN103109767A (en) | Loach fish fry artificial cultivation method | |
| CN107581106B (en) | A kind of artificial fecundation method of platax orbicularis | |
| CN105746411B (en) | A kind of foster method of bottom of pond kind blueness cray fingerling company | |
| CN102599091A (en) | Hybrid propagation method and fertilized ovum hatching device for loach | |
| CN103875566A (en) | Method for ecologically breeding portunus trituberculatus in ponds | |
| CN103444600A (en) | Two-stage efficient rearing method for freshwater shrimp fry | |
| CN104472412A (en) | Coilia ectenes step type breeding method | |
| CN102870722B (en) | Large scale larva breeding method for navodon septentrionalis | |
| CN102440209A (en) | Method for incubating oriental weatherfish | |
| CN101243778B (en) | Method for cultivating Protosalanx hyalocranius fingerlings | |
| CN101103706A (en) | Reproduction method for American reeves shad artificial cultivation parent fish | |
| CN102144596B (en) | Method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating | |
| CN103493757B (en) | Method for breeding and cultivating four generations of fish and shrimp seedlings in pond in one year | |
| CN105918168B (en) | Exopalaemon carinicauda saline-alkali water artificial cultivating method | |
| CN103493767B (en) | Odontobulis mpotamophila and Odontobutis yaluensis cross breeding method | |
| CN101444192B (en) | Sebastiscus marmoratus industrialized fry breeding method | |
| CN103416325A (en) | Technique of rearing fry of obscure puffer in low salinity sea water | |
| CN102919186B (en) | Artificial breeding method for sillago sihama | |
| CN109699537A (en) | A kind of mating system of Stichopus variegatus (Sempen) artificial seed | |
| CN103444604A (en) | Small-water body fry rearing method for cromileptes altivelis | |
| CN202680227U (en) | Loach germ cell incubating device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130904 Termination date: 20160629 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |