CN102757489B - Method for synchronously extracting and separating arachin and conarrachin - Google Patents
Method for synchronously extracting and separating arachin and conarrachin Download PDFInfo
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Abstract
The invention discloses a method for synchronously extracting and separating arachin and conarrachin, Which comprises the following steps: evenly mixing peanut spent meal and a buffer solution, carrying out primary centrifugation, taking and standing the supernatant, carrying out secondary centrifugation on the supernatant in the system obtained by standing, and drying the precipitate to obtain the arachin; and regulating the pH value of the supernatant obtained by the secondary centrifugation to 4-6, carrying out ternary centrifugation, collecting the precipitate, adding water to uniformly disperse the precipitate, and drying to obtain the conarrachin. Compared with the existing method, the invention greatly simplifies the technological process, lowers the production cost of related products on the premise of ensuring the separation effect, and basically does not destroy the nutritive value of the proteins. The globulin content in the arachin separated by the method is up to 89.71%, and the separation purity is not less than 80%; the conarrachin content in the conarrachin is up to 92.52%, and the separation purity is not less than 70%.
Description
Technical field
The present invention relates to the separated arachin of a kind of simultaneous extraction and the method for accompanying sphaeroprotein (being also α-conarachin).
Background technology
Semen arachidis hypogaeae protein is divided into two large classes, be water-soluble protein and salting-in protein, wherein salting-in protein accounts for 90% left and right of gross protein, mainly comprise arachin (14S, account for 73% left and right), α-conarachin I (7.8S, account for 6% left and right), α-conarachin II (2S accounts for 18% left and right).There are some researches show, arachin is comprised of two acidics and three alkaline subunits, and α-conarachin only has a subunit, and 2S albumen is comprised of 6 polypeptide.At present, the extraction of Semen arachidis hypogaeae protein extensively adopts is alkali extraction and acid precipitation legal system for peanut protein isolate and alcohol wash legal system for peanut concentrated protein, it is mainly to utilize the principle of saltouing that ground-peanut ball/companion sphaeroprotein is prepared in separation, adopt the method for saturated ammonium sulphate fractionation precipitation, and be applicable to the method for separating and preparing that large-scale industrialization produces, have not yet to see relevant report.In addition, arachin and companion's sphaeroprotein have respectively unique physicochemical characteristic, such as viscosity, gelation, rheological, water holding, hold oiliness etc., therefore by the fractional separation to arachin and companion's sphaeroprotein, just likely the character of Semen arachidis hypogaeae protein different components is used respectively, to expanding the application of Semen arachidis hypogaeae protein in foodstuffs industry.
As far back as the seventies in last century, just carried out for glycinin (11S) in the world the research with the industrialization fractionation method of companion's sphaeroprotein (7S), current also comparative maturity and perfect of its separation-extraction technology, this has all possessed all theoretical investigationes for soybean protein classification component and product development to be expected to instruct or realize the possibility of industrialization.The separation of the protein ingredient that for example utilizes the difference of iso-electric point and carry out, the method is that regulator solution pH is carried out separation when approaching to Soy 11 S Globulin iso-electric point, so just can the higher Soy 11 S Globulin (JP 55-124457A) of DNA purity; Utilize and the reactive diverse ways of calcium, when soybean protein extracts, in solution, add a small amount of calcium salt, extract highly purified Soy 11 S Globulin (JP 48-56843A); Utilization is under certain pH and ionic concn condition, the method of soybean protein different fractions different solubility, be included in pH under 1.2 to 4.0 condition, in the soy bean proteinous soln that contains sodium-chlor or Repone K, soybean protein 7S component (JP49-31843A) is rich in preparation; Or regulate pH to 5.0 to 5.6 the sediment under iso-electric point condition, then the ionic strength of Chlorine in Solution sodium is adjusted to 0.01 to 0.2M, the soybean protein 7S that separation purity is higher and 11S component (JP58-36345A); Have one piece more similar with this patent, but be only applicable to the Patents of soybean protein components fractional separation, to utilize low-temperature sludge phenomenon and reductive agent to carry out fractional separation to soybean protein, under cold condition, reduce the solubleness of Soy 11 S Globulin, and in certain pH in solution, under the condition that hydrosulfide, paddy light ammonia peptide compounds or cysteine cpd exist, to processing in the aqueous solution of soybean protein, and then regulate pH to 5.5 to 7.0, just can isolate soybean protein 7S and 11S component (JP 61-187755A).The domestic and international fractional separation for ground-peanut ball/companion globulin fraction mainly adopts laboratory procedure---the saturated ammonium sulphate method of extraction on a small scale that is only applicable at present.Due to correlative study with the standby classification component of this legal system practical (industrialization), expect not obviously, and then affected the input of domestic and international investigator aspect Semen arachidis hypogaeae protein and component correlation theory and research and development of products.
Summary of the invention
The object of this invention is to provide the separated arachin of a kind of simultaneous extraction and the method for accompanying sphaeroprotein.
Separated in synchronization provided by the invention is prepared the method for arachin and α-conarachin, after comprising the steps: defatting peanut powder and damping fluid to mix, carry out centrifugal for the first time, get supernatant liquor standing, supernatant liquor in standing gained system is carried out centrifugal for the second time, after gained precipitation is dry, be arachin;
The pH value of centrifugal gained supernatant liquor is for the second time adjusted to again and carries out after 4-6 centrifugally for the third time, collecting precipitation, adds water and makes to precipitate dispersed, obtains described α-conarachin after dry.
In aforesaid method, in described defatting peanut powder, quality percentage composition≤5% of fat, NSI value >=60%;
The pH value of described damping fluid is 7.1-8.5, preferably 7.3-8.0; Phosphate concentration or hydrogen ion concentration are 0.1-2M, preferably 0.2-1M;
It is phosphoric acid buffer or the Tris-HCl damping fluid that 7.1-8.5, phosphate concentration are 0.1-2M that described damping fluid is specially pH value;
Described pH value is that the phosphoric acid buffer that 7.1-8.5, phosphate concentration are 0.1-2M is specially by Na
2hPO
412H
2o, NaH
2pO
42H
2o and water form.
The mass ratio of described defatting peanut powder and described damping fluid is (1-5): 10, preferably (2-4): 10, be specially 1: 10 or 3: 10 or 4: 10;
Described mixing in step, the time is 1-2 hour;
In described centrifugation step for the first time, temperature is 20-25 ℃, and centrifugal force is 4000-8000g, preferably 5000-7000g; Time is 15-40 minute, preferably 20-30 minute;
Described getting in the standing step of supernatant liquor, the time is 6-24 hour, preferably 8-20 hour; Temperature is 0-15 ℃, preferably 2-10 ℃;
In described centrifugation step for the second time, temperature is 0-15 ℃, and centrifugal force is 4000-12000g, preferably 5000-10000g; Time is 15-60 minute, preferably 20-50 minute;
In described centrifugation step for the third time, temperature is 20-25 ℃, and centrifugal force is 4000-8000g, preferably 5000-7000g; Time is 15-40 minute, preferably 20-30 minute.
Describedly in gained precipitation, add in the even step of water-dispersion, the consumption of water be gained precipitation quality 1-3 doubly.
In aforesaid method, drying step method therefor is ordinary method, as freezing or spray drying process, can choose according to practical situation.
In addition, the arachin obtaining according to the method described above and at least one in α-conarachin, also belong to protection scope of the present invention.
It is at least one in 40.5kDa, 37.5kDa, 35.5kDa and 23.5kDa arachin that described arachin specifically can be molecular weight, and α-conarachin is specially at least one in 61kDa, 18.1kDa, 17.0kDa, 16.3kDa and 15.5kDa α-conarachin.
The NSI value of described arachin is 90.3-96.2% or 90.3-92.6% or 92.6-96.2%, and the gel hardness of α-conarachin is 176.5-220.7g or 176.5-196.5g or 196.5-220.7g.
It is raw material that pulverous defatting peanut powder is take in the present invention, in the process of extraction, separated preparation, avoid as far as possible the Semen arachidis hypogaeae protein sex change causing under the conditions such as high-speed stirring, strong acid and strong base or heating, by weakly alkaline buffered soln, extract low-temperature sludge, the technology such as low-temperature centrifugation, arachin is extracted separated with α-conarachin, reach good separating effect, can utilize respectively the character that each component is different, carry out theoretical investigation or be applied to different food systems.And, inventor of the present invention studies discovery by experiment, through dsc (Differential Scanning Calorimetry, DSC) measure, adopt the enthalpy change value of arachin prepared by the method for the invention and α-conarachin higher than the enthalpy change value of peanut protein isolate or peanut concentrated protein respective components, illustrate that operation of the present invention can protect the unlikely serious sex change of Semen arachidis hypogaeae protein component natural structure; Research also finds that arachin component has good solubility, and the far super commercialization soybean protein isolate of gel hardness of the prepared heat-induced gelation of α-conarachin, character and peanut protein isolate that Semen arachidis hypogaeae protein one-component is described have very large difference, can for different food systems, research and develop and apply according to different components advantage physicochemical characteristic.
The present invention has simplified technical process than existing methods greatly, has reduced the production cost of related products when guaranteeing separating effect, does not substantially destroy the nutritive value of protein.Utilize the sphaeroprotein content in the separated arachin of the method can reach 89.71%, separation purity is not less than 80%, and the α-conarachin content in α-conarachin can reach 92.52%, and separation purity is not less than 70%.
Accompanying drawing explanation
Fig. 1 is for adopting the method for the invention to extract the SDS-PAGE collection of illustrative plates of separated ground-peanut ball/α-conarachin with saturated ammonium sulphate method.
Fig. 2 is for adopting differential scanning calorimeter (DSC) to measure the α-conarachin (A) of the separated preparation of the method for the invention and the corresponding collection of illustrative plates of arachin (B).
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Described method is ordinary method if no special instructions.Described starting material all can obtain from open commercial sources if no special instructions.
The detection method relating in following embodiment is all as follows:
When defatting peanut powder is detected, the detection method of nitrogen soluble index (NSI) is carried out with reference to the soybean water-soluble protein determination method of GB5511-85 appendix A.The concrete steps of this measuring method are: accurately take sample 5.00g in ground tool plug Erlenmeyer flask, add water 200ml, shake up make dispersed, then at 25-30 ℃ of temperature, shake 2h, after taking-up, mixed solution is transferred in 250ml volumetric flask, be diluted with water to scale, mix rear standing 1-2min, supernatant liquid is poured in centrifuge tube, the centrifugal 10min of 1500 * g, then supernatant liquor is filtered with quick filter paper or glass fibre, clear liquid collected in Erlenmeyer flask, get 10ml liquid and carry out Kjeldahl determination, calculate the protein content in the aqueous solution.NSI calculation formula is as follows: nitrogen soluble index (NSI)=(in the aqueous solution before protein total content/measurement in solid protein quality) * 100%.
The preparation of protein gel and the mensuration of gel hardness thereof are specific as follows: arachin, α-conarachin solution that preparation protein concentration (m/V) is 14%, stir 120min under room temperature.Respectively get 20mL solution in 50ml beaker, with preservative film sealing, be placed at 90 ℃ heating in water bath 2 hours.After taking-up, with flowing water, be cooled to after room temperature rapidly, be placed in 4 ℃ of refrigerator overnight 14h, make protein gelatin.Using TA-TX2i matter structure instrument (probe type is P0.5R, and diameter is 12mm) to measure its gel hardness at 25 ℃ of room temperatures, measurement result employing TPA-macro instrument carries out the hardness of analytical calculation protein gelatin body.
The mensuration of protein quality (content) is all to carry out based on Kjeldahl method, measures nitrogen content and be multiplied by coefficient 5.46 to be converted to Semen arachidis hypogaeae protein quality (content).
Protein extracting ratio is the per-cent that the Semen arachidis hypogaeae protein quality that can be extracted in defatting peanut powder sample accounts for total protein in defatting peanut powder.
Adopt saturated ammonium sulphate to saltout to prepare the extraction and separation method of arachin and α-conarachin as follows: degreased peanut flour adds the NaCl containing 0.5mol/L in the ratio of 1: 20, in the phosphate buffer soln of pH 7.9, at room temperature stir after 2h, in 10000 * g, centrifugal 30min at 20 ℃.Supernatant liquor adds ammonium sulfate to make solution saturation ratio reach 40%, at 4 ℃, stirs after 1h, then in 10000 * g, and centrifugal 30min at 4 ℃, precipitation is the ratio redissolution by 1: 5 with above-mentioned phosphate buffered saline buffer, after dialyse 48h lyophilize, obtains arachin.Supernatant liquor after centrifugal adds ammonium sulfate to make its saturation ratio reach 60%, at 4 ℃, stirs after 1h, and in 10000 * g, centrifugal 30min at 4 ℃.Gained supernatant liquor continues to add ammonium sulfate to make solution saturation ratio reach 85%, at 4 ℃, stir after 1h, in 10000 * g, centrifugal 30min at 4 ℃, gained precipitation is redissolved in the ratio of 1: 5 with phosphate buffered saline buffer, after dialysis 48h lyophilize, obtains α-conarachin.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is with reference to the method for Laemmli (1970).Concentrated gum concentration is 5%, and resolving gel concentration is 13%, before electrophoresis sample introduction, sample solution and sample buffer concussion is mixed, and boils 5min, cooling rapidly, and centrifugal 5min, and electrophoresis applied sample amount is 10 μ L.Gel electrophoresis is carried out under constant voltage, and in concentrated glue, voltage is 80V, increases to 110V after entering separation gel.While running extremely apart from glass plate edge 1cm left and right in electrophoresis forward position, stop electrophoresis.Gel film is taken out and to put into appropriate coomassie brilliant blue R250 dyeing 1h, then decolour with methyl alcohol and acetic acid mixed solution, during change destainer 2-3 time, when clear to protein spectra till.
Ground-peanut ball (or companion's ball) albumen sepn purity refers to ratio or the per-cent of ground-peanut ball in the arachin product finally preparing (or companion's ball) albumen/(arachin+α-conarachin).The bands of a spectrum that specifically obtain by above-mentioned SDS-PAGE electrophoresis are by spectrodensitometry, and purity represents (%) with the per-cent of corresponding band area and the total area.The content of described arachin refers to that molecular weight is the total amount of 40.5kDa, 37.5kDa, 35.5kDa and 23.5kDa subunit band, and the content of α-conarachin refers to 61kDa, 18.1kDa, 17.0kDa, 16.3 and the total amount of 15.5kDa subunit band.
The mensuration of enthalpy change value adopts dsc (Differential Scanning Calorimetry, DSC).Specifically prepare 10% sphaeroprotein or α-conarachin dispersion liquid and at room temperature place after 1h, draw 10 μ L albumen dispersion liquids to aluminium box, platen, take blank panel as contrast.Temperature scanning scope: 0-120 ℃; Temperature rise rate: 10 ℃/min; Protection gas nitrogen flow rate: 50mL/min.Adopt the sex change enthalpy change value of the universal analyzer 2000 computed in software protein.All experimental results are the mean value of three measured values.
Embodiment 1:
By the defatting peanut powder that fatty quality percentage composition is 4%, NSI value is 60%, according to mass ratio 1: 10, in pH 8.0, phosphate concentration, be 0.4M by Na
2hPO
412H
2o and NaH
2pO
42H
2in the phosphate buffer solution that O and water form, fully mix and stir after 1h, 25 ℃ of 8000 * g carry out centrifugal 20 minutes for the first time, getting supernatant liquor, to be placed on temperature be standing 12h in the environment of 4 ℃, and then with cryogenic freezing whizzer at 4 ℃, under 8000 * g condition, carry out centrifugal 30 minutes for the second time, collect centrifugal gained precipitation, in this precipitation, add 3 times to the deionized water of its quality, dispersedly by lyophilize, obtain arachin powder-like product;
After again the pH value of centrifugal gained supernatant liquor being adjusted to 4.5, under 20 ℃, 6000 * g condition, carry out centrifugal 20 minutes for the third time, the dispersed rear homogeneous lyophilize of directly carrying out of deionized water by gained precipitation (being also curdled milk) with 3 times of its quality, obtains α-conarachin powder-like product.
Described defatting peanut powder and damping fluid mix in whipping step, and damping fluid is 70.85% to the extraction yield of Semen arachidis hypogaeae protein, and showing to add up in the contained Semen arachidis hypogaeae protein of this embodiment defatting peanut powder used 70.85% Semen arachidis hypogaeae protein can be by this buffer extraction.
After measured, the quality percentage composition of the arachin in this embodiment gained arachin powder-like product is 85.61% (butt), according to method shown in embodiment 3, carry out SDS-PAGE collection of illustrative plates mensuration, the separation purity that obtains arachin is 85.7%;
The quality percentage composition of α-conarachin in α-conarachin powder-like product is 89.61% (butt), according to method shown in embodiment 3, carries out SDS-PAGE collection of illustrative plates mensuration, and the separation purity that obtains α-conarachin is 75.1%.
The NSI value of arachin is 90.3%, and the gel hardness of α-conarachin is 196.5g.
In addition, this embodiment prepares the sex change peak enthalpy change value of gained α-conarachin and arachin and embodiment 3 acquired results without substantive difference, repeats no more, and shows that the denaturation degrees of α-conarachin and arachin is all very little.
Embodiment 2:
By fatty quality percentage composition, be 3%, NSI value is 65% defatting peanut powder according to mass ratio 3: 10, in pH7.9, hydrogen ion concentration is in the Tris-HCl buffered soln of 0.1M, fully mix and stir after 1h, 20 ℃ of 6000 * g carry out centrifugal 30 minutes for the first time, getting supernatant liquor, to be placed on temperature be standing 24h in the environment of 7 ℃, then using cryogenic freezing whizzer at 10 ℃, under 8000 * g condition, carry out centrifugal 40 minutes for the second time, collect centrifugal gained precipitation, this precipitation is scattered in the deionized water of its 2 times of quality, through lyophilize, obtain arachin powder-like product,
After again the pH value of centrifugal gained supernatant liquor being adjusted to 4.0, under 25 ℃, 7000 * g condition, carry out centrifugal 30 minutes for the third time, the dispersed rear lyophilize of directly carrying out of deionized water by gained precipitation (being also curdled milk) with its 2.5 times of quality, obtains α-conarachin powder-like product.
Described defatting peanut powder and damping fluid mix in whipping step, and damping fluid is 66.12% to the extraction yield of Semen arachidis hypogaeae protein, and showing to add up in the contained Semen arachidis hypogaeae protein of this embodiment defatting peanut powder used 66.12% Semen arachidis hypogaeae protein can be by this buffer extraction.
After measured, the quality percentage composition of the arachin in this embodiment gained arachin powder-like product is 88.30% (butt), according to method shown in embodiment 3, carry out SDS-PAGE collection of illustrative plates mensuration, the separation purity that obtains arachin is 83.2%;
The quality percentage composition of α-conarachin in α-conarachin powder-like product is 90.92% (butt), according to method shown in embodiment 3, carries out SDS-PAGE collection of illustrative plates mensuration, and the separation purity that obtains α-conarachin is 72.4%.
The NSI value of arachin is 92.6%, and the gel hardness of α-conarachin is 176.5g.
In addition, this embodiment prepares the sex change peak enthalpy change value of gained α-conarachin and arachin and embodiment 3 acquired results without substantive difference, repeats no more, and shows that the denaturation degrees of α-conarachin and arachin is all very little.
Embodiment 3:
By fatty quality percentage composition, be 1%, NSI value is 65% defatting peanut powder according to mass ratio 4: 10, in pH7.5, hydrogen ion concentration is in the Tris-HCl buffered soln of 0.2M, fully to mix to stir after 1h, 25 ℃ of 7000 * g carry out centrifugal 40 minutes for the first time, getting supernatant liquor, to be placed on temperature be standing 18h in the environment of 2 ℃, then with cryogenic freezing whizzer, under 2 ℃ of condition 10000 * g, carrying out centrifugal 50 minutes for the second time, collect centrifugal gained precipitation, this precipitation is at room temperature dissolved in appropriate deionized water, make its solution protein concentration 18%, the spray-dried arachin powder-like product that obtains,
After again the pH value of centrifugal gained supernatant liquor being adjusted to 5.0, under 20 ℃, 8000 * g condition, carry out centrifugal 40 minutes for the third time, isolate α-conarachin curdled milk, after add 3 times of deionized waters of curdled milk quality, adopt the NaOH solution of 2M that curdled milk suspension liquid pH value is adjusted to 7.0, the dry α-conarachin powder-like product that obtains of spraying.
Described defatting peanut powder and damping fluid mix in whipping step, and damping fluid is 64.27% to the extraction yield of Semen arachidis hypogaeae protein, and showing to add up in the contained Semen arachidis hypogaeae protein of this embodiment defatting peanut powder used 64.27% Semen arachidis hypogaeae protein can be by this buffer extraction.
Fig. 1 is that this embodiment method of employing is extracted the SDS-PAGE collection of illustrative plates of separated ground-peanut ball/α-conarachin with saturated ammonium sulphate method.As shown in Figure 1, sample 1,2 is respectively arachin and the α-conarachin that adopts saturated ammonium sulphate method to obtain, and because its separation purity is high, can be used as standard control sample.Sample 3,4 are respectively arachin and the α-conarachin that adopts this embodiment method to prepare, the band of electrophoresis subunit shown in its figure can control sample 1,2 corresponding position bands are determined ownership, by densitometric scan, analyze, in confirmatory sample 3,40.5kDa, 37.5kDa, 35.5kDa and 23.5kDa place band account for respectively 20.7%, 18.7%, 11.7% and 34.3% of all band total amounts, and adding with the separation purity of rear total arachin is 85.4%;
61kDa, 18.1kDa in sample 4,17.0kDa, 16.3 and 15.5kDa place band account for respectively 41.7%, 6.6%, 8.6%, 12.1% and 9.4% of all band total amounts, adding with the separation purity of rear total arachin is 78.4%.
Fig. 2 is for adopting differential scanning calorimeter (DSC) to measure the α-conarachin (A) of the separated preparation of the method for the invention and the corresponding collection of illustrative plates of arachin (B).The arachin phenomenon that solubleness reduces at low temperatures, only in its structure in not occurring under denatured state, and the phenomenon that this low temperature cold is heavy is a hot reversing process, cold at low temperatures heavy protein rises to after normal temperature in temperature, protein still can redissolve, and protein structure character does not change still in denatured state not.In DSC spectrogram, enthalpy change value is higher illustrates that the denaturation degrees of respective egg white matter is less.As shown in the figure, α-conarachin and sex change peak, arachin place enthalpy change value are respectively 4.56 ± 0.03J/g and 18.65 ± 0.44J/g, have less denaturation degrees.
After measured, the quality percentage composition of the arachin in this embodiment gained arachin powder-like product is 87.21% (butt);
The quality percentage composition of α-conarachin in α-conarachin powder-like product is 92.36% (butt).
The NSI value of arachin is 96.2%, and the gel hardness of α-conarachin is 220.7g.
Claims (7)
1. the method for the separated arachin of a simultaneous extraction and α-conarachin, after comprising the steps: defatting peanut powder and damping fluid to mix, carry out centrifugal for the first time, get supernatant liquor standing, supernatant liquor in standing gained system is carried out centrifugal for the second time, after gained precipitation is dry, be arachin; Described damping fluid is that pH value is that 7.1-8.5, phosphate concentration or hydrogen ion concentration are phosphoric acid buffer or the Tris-HCl damping fluid of 0.1-2M; The mass ratio of described defatting peanut powder and described damping fluid is (1-5): 10;
The pH value of centrifugal gained supernatant liquor is for the second time adjusted to again and carries out after 4-6 centrifugally for the third time, collecting precipitation, adds water and makes to precipitate dispersed, obtains described α-conarachin after dry;
Described mixing in step, the time is 1-2 hour;
In described centrifugation step for the first time, temperature is 20-25 ℃, and centrifugal force is 4000-8000g, and the time is 15-40 minute;
Described getting in the standing step of supernatant liquor, the time is 6-24 hour; Temperature is 0-15 ℃;
In described centrifugation step for the second time, temperature is 0-15 ℃, and centrifugal force is 4000-12000g; Time is 15-60 minute;
In described centrifugation step for the third time, temperature is 20-25 ℃, and centrifugal force is 4000-8000g; Time is 15-40 minute.
2. method according to claim 1, is characterized in that: in described defatting peanut powder, and quality percentage composition≤5% of fat, NSI value >=60%.
3. method according to claim 1 and 2, is characterized in that: described phosphoric acid buffer is by Na
2hPO
412H
2o, NaH
2pO
42H
2o and water form.
4. method according to claim 3, is characterized in that: the pH value of described damping fluid is 7.3-8.0; Phosphate concentration or hydrogen ion concentration are 0.2-1M.
5. method according to claim 4, is characterized in that: the mass ratio of described defatting peanut powder and described damping fluid is (2-4): 10.
6. method according to claim 5, is characterized in that: described in mix in step, the time is 1-2 hour;
In described centrifugation step for the first time, centrifugal force is 5000-7000g; Time is 20-30 minute;
Described getting in the standing step of supernatant liquor, the time is 8-20 hour; Temperature is 2-10 ℃;
In described centrifugation step for the second time, centrifugal force is 5000-10000g; Time is 20-50 minute;
In described centrifugation step for the third time, centrifugal force is 5000-7000g; Time is 20-30 minute.
7. method according to claim 6, is characterized in that: describedly in gained precipitation, add in the even step of water-dispersion, the consumption of water be gained precipitation quality 1-3 doubly.
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