CN102757489A - Method for synchronously extracting and separating arachin and conarrachin - Google Patents
Method for synchronously extracting and separating arachin and conarrachin Download PDFInfo
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- CN102757489A CN102757489A CN2012102559340A CN201210255934A CN102757489A CN 102757489 A CN102757489 A CN 102757489A CN 2012102559340 A CN2012102559340 A CN 2012102559340A CN 201210255934 A CN201210255934 A CN 201210255934A CN 102757489 A CN102757489 A CN 102757489A
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Abstract
The invention discloses a method for synchronously extracting and separating arachin and conarrachin, Which comprises the following steps: evenly mixing peanut spent meal and a buffer solution, carrying out primary centrifugation, taking and standing the supernatant, carrying out secondary centrifugation on the supernatant in the system obtained by standing, and drying the precipitate to obtain the arachin; and regulating the pH value of the supernatant obtained by the secondary centrifugation to 4-6, carrying out ternary centrifugation, collecting the precipitate, adding water to uniformly disperse the precipitate, and drying to obtain the conarrachin. Compared with the existing method, the invention greatly simplifies the technological process, lowers the production cost of related products on the premise of ensuring the separation effect, and basically does not destroy the nutritive value of the proteins. The globulin content in the arachin separated by the method is up to 89.71%, and the separation purity is not less than 80%; the conarrachin content in the conarrachin is up to 92.52%, and the separation purity is not less than 70%.
Description
Technical field
The present invention relates to a kind of simultaneous extraction and separate arachin and the method for accompanying sphaeroprotein (also being Ban Huashengqiudanbai).
Background technology
Semen arachidis hypogaeae protein is divided into two big types, i.e. water-soluble protein and salting-in protein, and wherein salting-in protein accounts for about 90% of gross protein; Mainly comprise arachin (14S; Account for about 73%), Ban Huashengqiudanbai I (7.8S accounts for about 6%), Ban Huashengqiudanbai II (2S accounts for about 18%).There are some researches show that arachin is made up of two acid subunits and three alkaline subunits, Ban Huashengqiudanbai only has a subunit, and 2S albumen is made up of 6 polypeptide.At present; The extraction of Semen arachidis hypogaeae protein is extensively adopted is that the alkali extraction and acid precipitation legal system is equipped with peanut protein isolate and alcohol and washes legal system and be equipped with peanut concentrated protein; Separating preparation ground-peanut ball/companion's sphaeroprotein then mainly is to utilize the principle of saltouing; Adopt the method for saturated ammonium sulphate fractionation precipitation, and be applicable to that the method for separating and preparing that large-scale industrialization is produced do not see relevant report yet.In addition; Arachin has unique physicochemical characteristic respectively with companion's sphaeroprotein; For example viscosity, gelation, rheological, water holding are held oiliness etc.; Therefore through to the fractional separation of arachin, just might the character of Semen arachidis hypogaeae protein different components be used respectively, in the hope of enlarging Semen arachidis hypogaeae protein in Application in Food Industry with companion's sphaeroprotein.
As far back as the seventies in last century; Just carried out in the world to the research of glycinin (11S) with the industriallization fractionation method of companion's sphaeroprotein (7S); Present also comparative maturity and perfect of its separation-extraction technology, this makes all theoretical investigationes that are directed against Sunlover 10 classification component and product development all possess the possibility that is expected to instruct or realize industrialization.The separation of the protein ingredient that for example utilizes the difference of iso-electric point and carry out; This method is that regulator solution pH separates it when approaching to soybean 11S sphaeroprotein iso-electric point, so just can dna purity than higher soybean 11S sphaeroprotein (JP 55-124457A); Utilize and the reactive diverse ways of calcium, when Sunlover 10 extracts, in solution, add a spot of calcium salt, extract highly purified soybean 11S sphaeroprotein (JP 48-56843A); Be utilized under certain pH and the ionic concn condition; The method of Sunlover 10 different fractions different solubility; Be included in pH under 1.2 to 4.0 condition, contain in the soy bean proteinous soln of sodium-chlor or Repone K preparation and be rich in Sunlover 10 7S component (JP49-31843A); Perhaps the sediment under the iso-electric point condition is regulated pH to 5.0 to 5.6, the ionic strength with sodium-chlor in the solution is adjusted to 0.01 to 0.2M then, Sunlover 10 7S that separation purity is higher and 11S component (JP58-36345A); Have one piece more similar with this patent; But be only applicable to the related patent U.S. Patent No. of soybean protein components fractional separation; Be to utilize low-temperature sludge phenomenon and reductive agent that Sunlover 10 is carried out fractional separation, under coldcondition, reduce the solubleness of soybean 11S sphaeroprotein, and in certain pH in the solution; Under the condition that hydrosulfide, paddy light ammonia peptide compounds or cysteine cpd exist; Handle in the aqueous solution to Sunlover 10, and then regulate pH to 5.5, just can isolate Sunlover 10 7S and 11S component (JP 61-187755A) to 7.0.The fractional separation that is directed against ground-peanut ball/companion's globulin fraction at present both at home and abroad mainly adopts laboratory procedure---the saturated ammonium sulphate method of extraction on a small scale that is only applicable to.Owing to correlative study practical (industrialization) expection of the classification component for preparing with this method is not obvious, and then influenced the input of domestic and international investigator aspect Semen arachidis hypogaeae protein and component correlation theory and research and development of products.
Summary of the invention
The purpose of this invention is to provide a kind of simultaneous extraction and separate arachin and the method for accompanying sphaeroprotein.
Separated in synchronization provided by the invention prepares the method for arachin and Ban Huashengqiudanbai; Comprise the steps: carrying out the first time behind defatting peanut powder and the damping fluid mixing centrifugal; Getting supernatant leaves standstill; To carry out the second time centrifugal with leaving standstill supernatant in the gained system, is arachin after the gained deposition drying;
For the second time the pH value of centrifugal gained supernatant is adjusted to and carries out behind the 4-6 centrifugally for the third time again, and collecting precipitation adds water to make the deposition homodisperse, obtains said Ban Huashengqiudanbai after the drying.
In the aforesaid method, in the said defatting peanut powder, quality percentage composition≤5% of fat, NSI value>=60%;
The pH value of said damping fluid is 7.1-8.5, preferred 7.3-8.0; Phosphate concentration or Sorensen value are 0.1-2M, preferred 0.2-1M;
Said damping fluid is specially the pH value is 0.1-2M for 7.1-8.5, phosphate concentration phosphoric acid buffer or Tris-HCl damping fluid;
Said pH value is that 7.1-8.5, phosphate concentration are that the phosphoric acid buffer of 0.1-2M is specially by Na
2HPO
412H
2O, NaH
2PO
42H
2O and water are formed.
The mass ratio of said defatting peanut powder and said damping fluid is (1-5): 10, preferred (2-4): 10, be specially 1: 10 or 3: 10 or 4: 10;
In the said mixing step, the time is 1-2 hour;
In the said first time centrifugation step, temperature is 20-25 ℃, and cf-is 4000-8000g, preferred 5000-7000g; Time is 15-40 minute, preferred 20-30 minute;
The said supernatant of getting leaves standstill in the step, and the time is 6-24 hour, preferred 8-20 hour; Temperature is 0-15 ℃, preferred 2-10 ℃;
In the said second time centrifugation step, temperature is 0-15 ℃, and cf-is 4000-12000g, preferred 5000-10000g; Time is 15-60 minute, preferred 20-50 minute;
In the said centrifugation step for the third time, temperature is 20-25 ℃, and cf-is 4000-8000g, preferred 5000-7000g; Time is 15-40 minute, preferred 20-30 minute.
Said in gained precipitates, the adding in the even step of water-dispersion, the consumption of water is 1-3 a times of gained deposition quality.
In the aforesaid method, the drying step method therefor is ordinary method, like freezing or spray drying process, can choose according to practical situation.
In addition, at least a in arachin that obtains according to the method described above and the Ban Huashengqiudanbai also belongs to protection scope of the present invention.
It is at least a in 40.5kDa, 37.5kDa, 35.5kDa and the 23.5kDa arachin that said arachin specifically can be molecular weight, and Ban Huashengqiudanbai is specially at least a in 61kDa, 18.1kDa, 17.0kDa, 16.3kDa and the 15.5kDa Ban Huashengqiudanbai.
The NSI value of said arachin is 90.3-96.2% or 90.3-92.6% or 92.6-96.2%, and the gel hardness of Ban Huashengqiudanbai is 176.5-220.7g or 176.5-196.5g or 196.5-220.7g.
The present invention is a raw material with pulverous defatting peanut powder, extracting, separating in the process of preparation, avoids the Semen arachidis hypogaeae protein sex change that causes under the conditions such as high-speed stirring, strong acid and strong base or heating as far as possible; Extract low-temperature sludge, technology such as low-temperature centrifugation through weakly alkaline buffered soln; With arachin and Ban Huashengqiudanbai extraction separation; Reach separating effect preferably, can utilize the different character of each component respectively, carry out theoretical investigation or be applied to different food systems.And; Inventor of the present invention finds through experimental study; Through dsc (Differential Scanning Calorimetry; DSC) measure, adopt the enthalpy change value of arachin and the Ban Huashengqiudanbai of the method for the invention preparation to be higher than the enthalpy change value of peanut protein isolate or peanut concentrated protein respective components, explain that operation according to the invention can protect the unlikely serious sex change of Semen arachidis hypogaeae protein component natural structure; Research finds that also the arachin component has good solubility; And the far ultra commercialization soybean protein isolate of gel hardness of the prepared heat-induced gelation of Ban Huashengqiudanbai; Character and peanut protein isolate that the Semen arachidis hypogaeae protein one-component is described have very big difference, can research and develop and use to different food systems according to different components advantage physicochemical characteristic.
The present invention has simplified technical process than existing methods greatly, when guaranteeing separating effect, has reduced the production cost of related prods, does not destroy proteinic nutritive value basically.Utilize this method the sphaeroprotein content in the isolating arachin can reach 89.71%, separation purity is not less than 80%, the Ban Huashengqiudanbai content in the Ban Huashengqiudanbai can reach 92.52%, separation purity is not less than 70%.
Description of drawings
Fig. 1 is the SDS-PAGE collection of illustrative plates of the ground-peanut ball/Ban Huashengqiudanbai of employing the method for the invention and saturated ammonium sulphate method institute extraction separation.
Fig. 2 separates the Ban Huashengqiudanbai (A) of preparation and the corresponding collection of illustrative plates of arachin (B) for adopting DSC (DSC) to measure the method for the invention.
Embodiment
Below in conjunction with specific embodiment the present invention is done further elaboration, but the present invention is not limited to following examples.Said method is ordinary method if no special instructions.Said starting material all can get from open commercial sources if no special instructions.
The detection method that relates among the following embodiment is all as follows:
When the defatting peanut powder was detected, the detection method of nitrogen soluble index (NSI) was carried out with reference to the soybean water-soluble protein determination method of GB5511-85 appendix A.The concrete steps of this measuring method are: accurately take by weighing sample 5.00g in ground tool plug Erlenmeyer flask, add water 200ml, shake up and make homodisperse; Under 25-30 ℃ of temperature, shake 2h then, after the taking-up mixed solution is transferred in the 250ml volumetric flask, be diluted with water to scale; Leave standstill 1-2min behind the mixing, supernatant liquid is poured in the centrifuge tube, the centrifugal 10min of 1500 * g; Again supernatant is filtered with quick filter paper or spun glass; Collect clear liquid in Erlenmeyer flask, get 10ml liquid and carry out Kjeldahl determination, calculate the protein contnt in the aqueous solution.The NSI calculation formula is following: nitrogen soluble index (NSI)=(in the aqueous solution before protein total content/measurement in the solid protein quality) * 100%.
The preparation of protein gel and the mensuration of gel hardness thereof are specific as follows: preparation protein concentration (m/V) is 14% arachin, Ban Huashengqiudanbai solution, stirs 120min under the room temperature.Respectively get 20mL solution in the 50ml beaker, seal with preservative film and be placed on 90 ℃ of following heating in water bath 2 hours.After being cooled to room temperature with flowing water rapidly after the taking-up, place 4 ℃ of refrigerator overnight 14h, process protein gelatin.Under 25 ℃ of room temperatures, measure its gel hardness at utilization TA-TX2i matter structure appearance (probe type is P0.5R, and diameter is 12mm), the mensuration result adopts the TPA-macro instrument to carry out the hardness of analytical calculation protein gelatin body.
The mensuration of protein quality (content) all is based on the Kjeldahl method carries out, and measures nitrogen content and multiply by coefficient 5.46 to convert Semen arachidis hypogaeae protein quality (content) into.
Protein extracting ratio is the per-cent that the Semen arachidis hypogaeae protein quality that can be extracted in the defatting peanut powder sample accounts for total protein in the defatting peanut powder.
Adopt the saltout extraction and separation method of preparation arachin and Ban Huashengqiudanbai of saturated ammonium sulphate following: degreased peanut flour contains 0.5mol/L NaCl in 1: 20 ratio adding; In the phosphate buffer soln of pH 7.9; After at room temperature stirring 2h, in 10000 * g, 20 ℃ of following centrifugal 30min.Supernatant adds ammonium sulfate makes the solution saturation ratio reach 40%, 4 ℃ stir 1h down after, again in 10000 * g, 4 ℃ of centrifugal 30min down, deposition obtains arachin with the ratio redissolution of above-mentioned phosphate buffered saline buffer by 1: 5 after dialyse 48h and the lyophilize.Supernatant after centrifugal adds ammonium sulfate makes its saturation ratio reach 60%, behind 4 ℃ of following stirring 1h, and in 10000 * g, 4 ℃ of following centrifugal 30min.The gained supernatant continues to add ammonium sulfate makes the solution saturation ratio reach 85%; Behind 4 ℃ of following stirring 1h, in 10000 * g, 4 ℃ of following centrifugal 30min; The gained deposition is redissolved in 1: 5 ratio with phosphate buffered saline buffer, obtains Ban Huashengqiudanbai after dialysis 48h and the lyophilize.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is with reference to the method for Laemmli (1970).Concentrated gum concentration is 5%, and resolving gel concentration is 13%, before the electrophoresis sample introduction, with sample solution and sample buffer concussion mixing, boils 5min, cooling, and centrifugal 5min rapidly, and the electrophoresis applied sample amount is 10 μ L.Gel electrophoresis is carried out under constant voltage, and voltage is 80V in concentrating glue, increases to 110V behind the entering separation gel.Stop electrophoresis when running extremely apart from the glass plate edge 1cm left and right sides in the electrophoresis forward position.Gel film taken out puts into an amount of coomassie brilliant blue R250 dyeing 1h, decolour with methyl alcohol and acetic acid mixed solution again, during change destainer 2-3 time, to protein spectra when clear till.
Ground-peanut ball (or companion's ball) albumen sepn purity is meant ratio or the per-cent of ground-peanut ball in the arachin product that finally prepares (or companion's ball) albumen/(arachin+Ban Huashengqiudanbai).Specifically be to pass through spectrodensitometry through the bands of a spectrum that above-mentioned SDS-PAGE electrophoresis obtains, purity is represented (%) with the per-cent of the corresponding band area and the total area.The content of said arachin is meant that molecular weight is the total amount of 40.5kDa, 37.5kDa, 35.5kDa and 23.5kDa subunit band, and the content of Ban Huashengqiudanbai is meant 61kDa, 18.1kDa, 17.0kDa, 16.3 and the total amount of 15.5kDa subunit band.
The mensuration employing dsc of enthalpy change value (Differential Scanning Calorimetry, DSC).Specifically be after preparation 10% sphaeroprotein or Ban Huashengqiudanbai dispersion liquid are at room temperature placed 1h, to draw 10 μ L albumen dispersion liquids to the aluminium box, platen is contrast with the blank panel.Temperature scanning scope: 0-120 ℃; Temperature rise rate: 10 ℃/min; Protection gas nitrogen flow rate: 50mL/min.Adopt the proteinic sex change enthalpy change of the universal analyzer 2000 computed in software value.All experimental results are the MV of three measured values.
Embodiment 1:
With the quality percentage composition of fat be 4%, the NSI value is 60% defatting peanut powder according to mass ratio 1: 10, in pH 8.0, phosphate concentration be 0.4M by Na
2HPO
412H
2O and NaH
2PO
42H
2After fully mixing stirs 1h in the phosphate buffer solution that O and water are formed; 25 ℃ of 8000 * g carry out centrifugal 20 minutes of the first time, and getting supernatant, to be placed on temperature be to leave standstill 12h in 4 ℃ the environment, and then with the freezing whizzer at 4 ℃; Carry out centrifugal 30 minutes of the second time under 8000 * g condition; Collect centrifugal gained deposition, in this deposition, add 3 times to the deionized water of its quality, homodisperse is after lyophilize obtains the arachin powder-like product;
After again the pH value of centrifugal gained supernatant being adjusted to 4.5; Under 20 ℃, 6000 * g condition, carried out centrifugal for the third time 20 minutes; Directly carry out the homogeneous lyophilize behind the deionized water homodisperse with gained deposition (also being curdled milk) and 3 times of its quality, obtain the Ban Huashengqiudanbai powder-like product.
In said defatting peanut powder and the damping fluid mixing whipping step, damping fluid is 70.85% to the extraction yield of Semen arachidis hypogaeae protein, show add up to 70.85% Semen arachidis hypogaeae protein in the contained Semen arachidis hypogaeae protein of the used defatting peanut powder of this embodiment can be by this buffer extraction.
Through measuring, the quality percentage composition of the arachin in this embodiment gained arachin powder-like product is 85.61% (butt), carries out the SDS-PAGE collection of illustrative plates according to method shown in the embodiment 3 and measures, and the separation purity that obtains arachin is 85.7%;
The quality percentage composition of Ban Huashengqiudanbai in the Ban Huashengqiudanbai powder-like product is 89.61% (butt), carries out the SDS-PAGE collection of illustrative plates according to method shown in the embodiment 3 and measures, and the separation purity that obtains Ban Huashengqiudanbai is 75.1%.
The NSI value of arachin is 90.3%, and the gel hardness of Ban Huashengqiudanbai is 196.5g.
In addition, sex change peak enthalpy change value and embodiment 3 gained results that this embodiment prepares gained Ban Huashengqiudanbai and arachin do not have substantive difference, repeat no more, and show that the denaturation degrees of Ban Huashengqiudanbai and arachin is all very little.
Embodiment 2:
With the quality percentage composition of fat be 3%, the NSI value is 65% defatting peanut powder according to mass ratio 3: 10; In pH7.9, Sorensen value is in the Tris-HCl buffered soln of 0.1M; After fully mixing stirred 1h, 20 ℃ of 6000 * g carried out centrifugal 30 minutes of the first time, and getting supernatant, to be placed on temperature be to leave standstill 24h in 7 ℃ the environment; Under 10 ℃, 8000 * g condition, carrying out centrifugal 40 minutes of the second time with the freezing whizzer then; Collect centrifugal gained deposition, this deposition is scattered in the deionized water of its 2 times of quality, obtain the arachin powder-like product through lyophilize;
After again the pH value of centrifugal gained supernatant being adjusted to 4.0; Under 25 ℃, 7000 * g condition, carried out centrifugal for the third time 30 minutes; Directly carry out lyophilize behind the deionized water homodisperse with gained deposition (also being curdled milk) and its 2.5 times of quality, obtain the Ban Huashengqiudanbai powder-like product.
In said defatting peanut powder and the damping fluid mixing whipping step, damping fluid is 66.12% to the extraction yield of Semen arachidis hypogaeae protein, show add up to 66.12% Semen arachidis hypogaeae protein in the contained Semen arachidis hypogaeae protein of the used defatting peanut powder of this embodiment can be by this buffer extraction.
Through measuring, the quality percentage composition of the arachin in this embodiment gained arachin powder-like product is 88.30% (butt), carries out the SDS-PAGE collection of illustrative plates according to method shown in the embodiment 3 and measures, and the separation purity that obtains arachin is 83.2%;
The quality percentage composition of Ban Huashengqiudanbai in the Ban Huashengqiudanbai powder-like product is 90.92% (butt), carries out the SDS-PAGE collection of illustrative plates according to method shown in the embodiment 3 and measures, and the separation purity that obtains Ban Huashengqiudanbai is 72.4%.
The NSI value of arachin is 92.6%, and the gel hardness of Ban Huashengqiudanbai is 176.5g.
In addition, sex change peak enthalpy change value and embodiment 3 gained results that this embodiment prepares gained Ban Huashengqiudanbai and arachin do not have substantive difference, repeat no more, and show that the denaturation degrees of Ban Huashengqiudanbai and arachin is all very little.
Embodiment 3:
With the quality percentage composition of fat be 1%, the NSI value is 65% defatting peanut powder according to mass ratio 4: 10; After pH7.5, Sorensen value are that fully mixing stirs 1h in the Tris-HCl buffered soln of 0.2M; 25 ℃ of 7000 * g carry out centrifugal 40 minutes of the first time, and getting supernatant, to be placed on temperature be to leave standstill 18h in 2 ℃ the environment, under 2 ℃ of condition 10000 * g, carrying out centrifugal 50 minutes of the second time with the freezing whizzer then; Collect centrifugal gained deposition; Should precipitate and at room temperature be dissolved in the proper amount of deionized water, and make its solution protein concentration 18%, the spray-dried arachin powder-like product that obtains;
After again the pH value of centrifugal gained supernatant being adjusted to 5.0; Under 20 ℃, 8000 * g condition, carried out centrifugal for the third time 40 minutes; Isolate the Ban Huashengqiudanbai curdled milk; The back adds 3 times of deionized waters of curdled milk quality, adopts the NaOH solution of 2M that curdled milk suspension liquid pH value is adjusted to 7.0, and spraying drying obtains the Ban Huashengqiudanbai powder-like product.
In said defatting peanut powder and the damping fluid mixing whipping step, damping fluid is 64.27% to the extraction yield of Semen arachidis hypogaeae protein, show add up to 64.27% Semen arachidis hypogaeae protein in the contained Semen arachidis hypogaeae protein of the used defatting peanut powder of this embodiment can be by this buffer extraction.
Fig. 1 is the SDS-PAGE collection of illustrative plates of the ground-peanut ball/Ban Huashengqiudanbai of this embodiment method of employing and saturated ammonium sulphate method institute extraction separation.As shown in Figure 1, sample 1,2 is respectively the arachin and the Ban Huashengqiudanbai of adopting the saturated ammonium sulphate method to obtain, because its separation purity is high, can be used as the standard control sample.Sample 3; 4 are respectively arachin and the Ban Huashengqiudanbai that this embodiment method of employing prepares; But electrophoresis subunit band control sample 1,2 corresponding position band is confirmed ownership shown in its figure, through the densitometric scan analysis; 40.5kDa in the confirmatory sample 3,37.5kDa, 35.5kDa and 23.5kDa place band account for 20.7%, 18.7%, 11.7% and 34.3% of all band total amounts respectively, and adding the separation purity that amounts to arachin with the back is 85.4%;
61kDa in the sample 4,18.1kDa, 17.0kDa, 16.3 and 15.5kDa place band account for 41.7%, 6.6%, 8.6%, 12.1% and 9.4% of all band total amounts respectively, adding the separation purity that amounts to arachin with the back is 78.4%.
Fig. 2 separates the Ban Huashengqiudanbai (A) of preparation and the corresponding collection of illustrative plates of arachin (B) for adopting DSC (DSC) to measure the method for the invention.Arachin is the phenomenon of solubleness reduction at low temperatures; Only be not in and could take place under the denatured state in its structure; And the cold heavy phenomenon of this low temperature is a hot reversing process; Promptly cold at low temperatures heavy protein is after temperature rises to normal temperature, and protein still can redissolve, and protein structure character does not change and still is in not denatured state.In the DSC spectrogram, the enthalpy change value is high more explains that then the denaturation degrees of respective egg white matter is more little.As shown in the figure, Ban Huashengqiudanbai and sex change peak, arachin place enthalpy change value are respectively 4.56 ± 0.03J/g and 18.65 ± 0.44J/g, have less denaturation degrees.
Through measuring, the quality percentage composition of the arachin in this embodiment gained arachin powder-like product is 87.21% (butt);
The quality percentage composition of Ban Huashengqiudanbai in the Ban Huashengqiudanbai powder-like product is 92.36% (butt).
The NSI value of arachin is 96.2%, and the gel hardness of Ban Huashengqiudanbai is 220.7g.
Claims (7)
1. a simultaneous extraction is separated the method for arachin and Ban Huashengqiudanbai; Comprise the steps: carrying out the first time behind defatting peanut powder and the damping fluid mixing centrifugal; Getting supernatant leaves standstill; To carry out the second time centrifugal with leaving standstill supernatant in the gained system, is arachin after the gained deposition drying;
For the second time the pH value of centrifugal gained supernatant is adjusted to and carries out behind the 4-6 centrifugally for the third time again, and collecting precipitation adds water to make the deposition homodisperse, obtains said Ban Huashengqiudanbai after the drying.
2. method according to claim 1 is characterized in that: in the said defatting peanut powder, and quality percentage composition≤5% of fat, NSI value>=60%.
3. method according to claim 1 and 2 is characterized in that: the pH value of said damping fluid is 7.1-8.5, preferred 7.3-8.0; Phosphate concentration or Sorensen value are 0.1-2M, preferred 0.2-1M;
Said damping fluid is specially the pH value is 0.1-2M for 7.1-8.5, phosphate concentration phosphoric acid buffer or Tris-HCl damping fluid;
Said pH value is that 7.1-8.5, phosphate concentration are that the phosphoric acid buffer of 0.1-2M is specially by Na
2HPO
412H
2O, NaH
2PO
42H
2O and water are formed.
4. according to the arbitrary described method of claim 1-3, it is characterized in that: the mass ratio of said defatting peanut powder and said damping fluid is (1-5): 10, preferred (2-4): 10.
5. according to the arbitrary described method of claim 1-4, it is characterized in that: in the said mixing step, the time is 1-2 hour;
In the said first time centrifugation step, temperature is 20-25 ℃, and cf-is 4000-8000g, preferred 5000-7000g; Time is 15-40 minute, preferred 20-30 minute;
The said supernatant of getting leaves standstill in the step, and the time is 6-24 hour, preferred 8-20 hour; Temperature is 0-15 ℃, preferred 2-10 ℃;
In the said second time centrifugation step, temperature is 0-15 ℃, and cf-is 4000-12000g, preferred 5000-10000g; Time is 15-60 minute, preferred 20-50 minute;
In the said centrifugation step for the third time, temperature is 20-25 ℃, and cf-is 4000-8000g, preferred 5000-7000g; Time is 15-40 minute, preferred 20-30 minute.
6. according to the described method of claim 1-5, it is characterized in that: said in gained precipitates, the adding in the even step of water-dispersion, the consumption of water is 1-3 a times of gained deposition quality.
7. at least a in the arachin that obtains of the arbitrary said method of claim 1-6 and the Ban Huashengqiudanbai.
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| CN103815431A (en) * | 2014-02-28 | 2014-05-28 | 中国农业科学院农产品加工研究所 | Sausage containing crosslinked peanut protein and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103815431A (en) * | 2014-02-28 | 2014-05-28 | 中国农业科学院农产品加工研究所 | Sausage containing crosslinked peanut protein and preparation method thereof |
| CN103815431B (en) * | 2014-02-28 | 2016-04-20 | 中国农业科学院农产品加工研究所 | A kind of sausage containing crosslinked peanut protein and preparation method thereof |
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| Publication number | Publication date |
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| CN102757489B (en) | 2014-01-29 |
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