CN102742896A - Grifola frondosus oral liquid and its production method - Google Patents
Grifola frondosus oral liquid and its production method Download PDFInfo
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- CN102742896A CN102742896A CN201110101559XA CN201110101559A CN102742896A CN 102742896 A CN102742896 A CN 102742896A CN 201110101559X A CN201110101559X A CN 201110101559XA CN 201110101559 A CN201110101559 A CN 201110101559A CN 102742896 A CN102742896 A CN 102742896A
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- oral liquid
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Abstract
The invention relates to the biological technology and food processing technical field, and concretely relates to a grifola frondosus oral liquid and its production method. According to the invention, a grifola frondosus broth is taken as a raw material, and is concentrated to obtain the oral liquid. The produced oral liquid keeps functional factor in the broth, the animal experiments prove that the oral liquid has the advantages of good anti-fatigue functional effect, good mouthfeel and wide market prospect.
Description
Technical field
The invention belongs to the food processing field of biological technical field and oral liquid.
Background technology
Modern disease is changing spectrum of disease to the mankind's threat; Require medical model to change to the pattern that prevention, health care, treatment, rehabilitation combine by simple disease treatment; Various alternative medicines and traditional medicine will be played a greater and greater role, and the world trends of " back to nature " that under this background, forms are just promoting international community growing to natural drug and nutrient drug demand.Therefore, the exploitation that has abundant nutrition, delicious flavour and have the fungi that health care is worth concurrently has good prospect.
It is antitumor to have reported that grifola frondosus has, anti HIV-1 virus, and antifatigue improves functions such as immunity; Receive people's favor, increasing in recent years grifola frondosus health products are also come out one after another, and comprise the grifola frondosus capsule; The grifola frondosus nutrient solution, ash tree jasmine tea, grifola frondosus pulvis or the like.Grifola frondosa oral liquid has very big exploitation and is worth as a kind of low dose of health beverages.
Summary of the invention
The object of the present invention is to provide a kind of Grifola frondosa oral liquid and preparation method thereof.
The object of the invention is realized through following technical scheme, being comprised:
((1) bacterial classification is cultivated: the PDA culture medium is processed flat board by conventional method.Take 1 of maitake mushroom mycelia after the activation of soya bean size with inoculation shovel, be forwarded on the PDA plating medium, 25 ℃ of constant temperature culture 15 days.
(2) seed liquor preparation: take 10 of maitake mushroom mycelias after the soya bean size is cultivated with the inoculation shovel, be inoculated in the 250mL baffle flask that 60mL potato-glucose-protein culture medium is housed, 25 ℃, the 140r/min shaking table is cultivated 100～110h.
(3) polyporus frondosus fermenting liquor preparation: seed fermentation liquid is inoculated in the fermentation tank by 10% inoculum concentration, 25 ℃ of cultivation temperature, initial PH is 5.5～6, incubation time 14 days, feed supplement midway is once.
(4) suction filtration: zymotic fluid obtains clarified solution and mycelium through the Buchner funnel suction filtration.
(5) concentrate: get polyporus frondosus fermenting liquor and steam in the bottle, revolve and steam 60～65 ℃ of temperature, fermentating liquid volume is condensed into the concentrate of variable concentrations in revolving.
(6) sterilization, canned.
Beneficial effect of the present invention:
The present invention all utilizes grifola frondosus fungi submerged fermentation liquid through filtering; Concentration obtains Grifola frondosa oral liquid; Retained various nutritional labelings in the grifola frondosus fungal fermented filtrate, realized the utilization fully of zymotic fluid function factor, product improves anti-fatigue ability for body and has remarkable result.
Specific embodiments
In order to understand the present invention, through embodiment the present invention is described further below, but does not limit the present invention.
Case study on implementation 1:
(1) bacterial classification is cultivated: the PDA culture medium is processed flat board by conventional method.Take 1 of maitake mushroom mycelia after the activation of soya bean size with inoculation shovel, be forwarded on the PDA plating medium, 25 ℃ of constant temperature culture 15 days.
(2) seed liquor preparation: take 10 of maitake mushroom mycelias after the soya bean size is cultivated with the inoculation shovel, be inoculated in the 250mL baffle flask that 60mL potato-glucose-protein culture medium is housed, 25 ℃, the 140r/min shaking table is cultivated 100h.
(3) polyporus frondosus fermenting liquor preparation: seed fermentation liquid is inoculated in the 7L fermentation tank by 10% inoculum concentration, and liquid amount is 5L, 25 ℃ of cultivation temperature, and initial PH is 5.5, incubation time 14 days replenishes glucose 80g once midway.
(4) suction filtration: zymotic fluid obtains clarified solution and mycelium through the Buchner funnel suction filtration.
(5) concentrate: get polyporus frondosus fermenting liquor and steam in the bottle in revolving, revolve and steam 60 ℃ of temperature, fermentating liquid volume is that original volume one half is for revolving the steaming terminal point in the bottle.
(6) sterilization, canned.
Case study on implementation 2:
(1) bacterial classification is cultivated: the PDA culture medium is processed flat board by conventional method.Take 1 of maitake mushroom mycelia after the activation of soya bean size with inoculation shovel, be forwarded on the PDA plating medium, 25 ℃ of constant temperature culture 15 days.
(2) seed liquor preparation: take 10 of maitake mushroom mycelias after the soya bean size is cultivated with the inoculation shovel, be inoculated in the 250mL baffle flask that 60mL potato-glucose-protein culture medium is housed, 25 ℃, the 140r/min shaking table is cultivated 110h.
(3) polyporus frondosus fermenting liquor preparation: seed fermentation liquid is inoculated in the 7L fermentation tank by 10% inoculum concentration, and liquid amount is 5L, 25 ℃ of cultivation temperature, and initial PH is 6, incubation time 14 days replenishes glucose 100g once midway.
(4) suction filtration: zymotic fluid obtains clarified solution and mycelium through the Buchner funnel suction filtration.
(5) concentrate: get polyporus frondosus fermenting liquor and steam in the bottle, revolve and steam 65 ℃ of temperature, be condensed into three times of concentrates in revolving.
(6) sterilization, canned.
Claims (2)
1. Grifola frondosa oral liquid and production method thereof is characterized in that may further comprise the steps:
(1) bacterial classification is cultivated: the PDA culture medium is processed flat board by conventional method.Take 1 of maitake mushroom mycelia after the activation of soya bean size with inoculation shovel, be forwarded on the PDA plating medium, 25 ℃ of constant temperature culture 15 days.
(2) seed liquor preparation: take 10 of maitake mushroom mycelias after the soya bean size is cultivated with the inoculation shovel, be inoculated in the 250mL baffle flask that 60mL potato-glucose-protein culture medium is housed, 25 ℃, the 140r/min shaking table is cultivated 100～110h.
(3) polyporus frondosus fermenting liquor preparation: seed fermentation liquid is inoculated in the fermentation tank by 10% inoculum concentration, 25 ℃ of cultivation temperature, initial PH is 5.5～6, incubation time 14 days, feed supplement midway is once.
(4) suction filtration: zymotic fluid obtains clarified solution and mycelium through the Buchner funnel suction filtration.
(5) concentrate: get polyporus frondosus fermenting liquor and steam in the bottle, revolve and steam 60～65 ℃ of temperature, fermentating liquid volume is condensed into the concentrate of variable concentrations in revolving.
(6) sterilization, canned.
(7) product has remarkable result for improving body resisting fatigue ability.
2. according to said a kind of Grifola frondosa oral liquid of claim 1 and production method thereof, it is characterized in that step (3) the grifola frondosus fermented and cultured cycle is long, need control fermentation parameter well, obtain the high-quality zymotic fluid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110101559XA CN102742896A (en) | 2011-04-22 | 2011-04-22 | Grifola frondosus oral liquid and its production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110101559XA CN102742896A (en) | 2011-04-22 | 2011-04-22 | Grifola frondosus oral liquid and its production method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102742896A true CN102742896A (en) | 2012-10-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201110101559XA Pending CN102742896A (en) | 2011-04-22 | 2011-04-22 | Grifola frondosus oral liquid and its production method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036445A (en) * | 2016-07-01 | 2016-10-26 | 河北大学 | Grifola frondosa flavored potato and jujube steamed sponge cakes and production method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1751694A (en) * | 2004-09-22 | 2006-03-29 | 中国食品发酵工业研究院 | Polyporus frondosus oral liquor and its prodn. method |
| CN100998611A (en) * | 2006-12-28 | 2007-07-18 | 温州大学 | Huishuhua fermentation preparation process using Chinese herb medicine as medium |
| CN101194917A (en) * | 2007-11-21 | 2008-06-11 | 贵州大学 | Grifola frondosa oral liquid rich in gastrodine, organic selenium and method for preparing the same |
-
2011
- 2011-04-22 CN CN201110101559XA patent/CN102742896A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1751694A (en) * | 2004-09-22 | 2006-03-29 | 中国食品发酵工业研究院 | Polyporus frondosus oral liquor and its prodn. method |
| CN100998611A (en) * | 2006-12-28 | 2007-07-18 | 温州大学 | Huishuhua fermentation preparation process using Chinese herb medicine as medium |
| CN101194917A (en) * | 2007-11-21 | 2008-06-11 | 贵州大学 | Grifola frondosa oral liquid rich in gastrodine, organic selenium and method for preparing the same |
Non-Patent Citations (2)
| Title |
|---|
| 马青等: "灰树花保健饮料加工工业的研究", 《食品与机械》 * |
| 黄小玲等: "灰树花保健饮料的研制", 《饮料工业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036445A (en) * | 2016-07-01 | 2016-10-26 | 河北大学 | Grifola frondosa flavored potato and jujube steamed sponge cakes and production method thereof |
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Application publication date: 20121024 |