CN102731204A - Preparation method of foliar fertilizer promoting alfalfa seedling growth in acidic soil - Google Patents

Preparation method of foliar fertilizer promoting alfalfa seedling growth in acidic soil Download PDF

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CN102731204A
CN102731204A CN2012101969634A CN201210196963A CN102731204A CN 102731204 A CN102731204 A CN 102731204A CN 2012101969634 A CN2012101969634 A CN 2012101969634A CN 201210196963 A CN201210196963 A CN 201210196963A CN 102731204 A CN102731204 A CN 102731204A
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preparation
nutrient solution
growth
foliage fertilizer
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CN102731204B (en
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安渊
周鹏
王生垠
高小燕
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North Jiangsu Institute Of Shanghai Jiaotong University
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Shanghai Jiaotong University
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Abstract

The invention discloses a preparation method of a foliar fertilizer promoting alfalfa seedling growth in acidic soil. The method comprises: first preparing a root growth solution, a mineral nutrient solution and an organic nutrient solution, then respectively taking 1-2ml of the root growth solution, 1-2ml of the mineral nutrient solution, and 3-5ml of the organic nutrient solution, mixing them and adding water to a constant volume to 1L, thus forming the foliar fertilizer. The foliar fertilizer prepared by the invention can effectively promote the growth of alfalfa seedling roots in strongly acidic soil, protect root activity and cell structures, relieve alfalfa seedling poisoning, and promote alfalfa growth. In a soil environment with pH of 4.5, the foliar fertilizer of the invention is utilized to treat alfalfa having an average plant height of 5cm. The foliar fertilizer is sprayed once every 10 days and 2 times in total. 1-2L/m<2> of the fertilizer is sprayed every time. After 30 days of treatment, the alfalfa root biomass is increased by 1.5-1.8 times compared with a control one, and the aboveground biomass is increased by 1.0-1.4 times compared with the control one.

Description

A kind of foliage fertilizer preparation method who promotes acid soil alfalfa growth of seedling
Technical field
What the present invention relates to is a kind of leaf leaven of agricultural technology field, specifically is a kind of foliage fertilizer preparation method who improves acid soil alfalfa growth of seedling.
Background technology
Red Soils in Southern China district soil generally presents strongly-acid (pH < 5.0); The g and D that suppresses the alfalfa root system, tip of a root passivation, the root hair is short and thick; The dietetic alimentation function reduces; And then suppress the alfalfa growth, cause that alfalfa plants is short, poor growth, output be very low, seriously restricted the geographic alfalfa plantation of Southern Red Soil soil.
At present, the relevant alfalfa that promotes is fewer at acid soil Study on Growth report, does not still have the otherwise effective technique measure.Literature search through to prior art finds that Guo Yanjun has reported that at " grass cultivation journal " the 15th volume 1 phase (84-89 page or leaf) in acid soil, adding lime regulates soil pH value, has obviously promoted the growth of alfalfa with yellow found the state (2006); Wang Xinhai etc. (2005) have reported to mend in the survey article of " Yunnan Prov Agriculture University's journal " the 20th volume 5 phases (705-709 page or leaf) and have executed the growth that boron fertilizer can improve roots of alfalfa in the acid soil.But be to use the lime cost of improving the soil very high, the feasibility that big area is used is low; And it is very limited to the amount of increase in production that promotes the acid soil clover to use boron fertilizer separately, and therefore, these two kinds of methods all are not widely used in actual production, does not find a kind of technical measures that can effectively promote the growth of acid soil alfalfa at present as yet.
Also find in the retrieval; One Chinese patent application number is: 200910135796.0; Denomination of invention is: a kind of efficient green foliage fertilizer and method for making thereof, this invention are a kind of efficient green foliage fertilizers: " it forms (in ton) by following proportioning: sodium nitrophenolate 8-10kg, α-Nai Yisuanna 2-4kg, an ammonium nitrate 70-90kg, Tripotassium Citrate 70-90kg, trace element (in metal) 9-12kg, choline dichloride 40-60kg, kaolin surplus.At first that sodium nitrophenolate, α-Nai Yisuanna and Tripotassium Citrate is even in stirrer for mixing, add other raw material then and evenly promptly get product of the present invention in stirrer for mixing.It has onset rapidly, the distinguishing feature of increase yield significantly, safety nuisance free." but this invention can't be used to promote acid soil alfalfa growth of seedlings.
Summary of the invention
The present invention is directed to the deficiency and the problem that exist in the background technology, a kind of preparation method who improves the foliage fertilizer of acid soil alfalfa growth of seedling is provided.The foliage fertilizer of the inventive method preparation is at China's soils in south china severe acidification (pH < 5.0); The khoai that alfalfa growing receives obvious inhibition uses; Can effectively promote the g and D of alfalfa seedlings root; Improve the absorptive function of root system, promote the growth of acid soil alfalfa nutrition.
The present invention realizes through following technical scheme:
Foliage fertilizer of the present invention, by root growth liquid, mineral nutrition liquid; Organic nutrient solution for cultivating and clear water mix to be formed; Contain root growth liquid 1-2ml in every liter of this foliage fertilizer, mineral nutrition liquid 1-2ml, organic nutrient solution for cultivating 3-5ml; Clear water is a surplus, and wherein: described root growth liquid is made up of indole-3-acetic acid (IAA); Said mineral nutrition liquid is made up of zinc (Zn), calcium (Ca), boron (B); Said organic nutrient solution for cultivating is made up of succsinic acid, Hydrocerol A, NOVASOY 400;
Above-mentioned foliage fertilizer prepares through following steps:
1) preparation of root growth liquid:
(1) takes by weighing and draw indole-3-acetic acid (IAA), be dissolved in ethanol until dissolving fully;
(2) with the IAA solution of said (1) preparation, be settled to 1 liter (L), form described root growth liquid with clear water.
2, the preparation of mineral nutrition liquid:
(1) takes by weighing the ZnPO that contains zinc (Zn) respectively 3The CaPO of calcic (Ca) 3, boric acid;
(2) with the ZnPO in said (1) 3, CaPO 3Be dissolved in fully in the clear water,
(3) boric acid is added the ZnPO in said (2) 3And CaPO 3In the mixed solution, be settled to 1 liter (L), the described mineral nutrition liquid of mineral with clear water.
3, the preparation of organic nutrient solution for cultivating:
(1) gets succsinic acid, Hydrocerol A, NOVASOY 400 respectively;
(2) succsinic acid in said (1), Hydrocerol A and NOVASOY 400 are mixed, be settled to 1L, form described organic nutrient solution for cultivating with clear water.
4, foliage fertilizer preparation:
Get root growth liquid, mineral nutrition liquid and organic nutrient solution for cultivating respectively, be settled to 1L, form a kind of foliage fertilizer that improves acid soil alfalfa growth of seedling with its mixing and with clear water.
Further, described step 1) is specially:
(1) the pure article that take by weighing 0.7g draw indole-3-acetic acid (IAA) (or non-pure article IAA of suitable content), are dissolved in a small amount of 95% ethanol until dissolving fully;
(2) with the IAA solution of said (1) preparation, be settled to 1 liter (L), form described root growth liquid, contain IAA:4mmol/L in every liter of this root growth liquid with clear water.
Further, described step 2), be specially:
(1) takes by weighing the ZnSO that contains 65g zinc (Zn) respectively 4169g; The MgSO that contains 96g magnesium (Mg) 4512g; The MnCl that contains 55g manganese (Mn) 2126g;
(2) with the ZnSO in said (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described mineral nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this mineral nutrition liquid.
Further, described step 3) is specially:
(1) gets the pure succsinic acid of 2.4g (or non-pure article succsinic acid of suitable content), the pure Hydrocerol A of 3.8g (or non-pure article Hydrocerol A of suitable content) and the pure NOVASOY 400 of 4.4g (or non-pure article NOVASOY 400 of suitable content) respectively;
(2) succsinic acid in said (1), Hydrocerol A and NOVASOY 400 are mixed; Be settled to 1L with clear water; Form described organic nutrient solution for cultivating, contain succsinic acid: 20mmol/L, Hydrocerol A: 20mmol/L and NOVASOY 400 in every liter of this organic nutrient solution for cultivating respectively: 10 μ mol/L.
Further, described step 4) is specially:
Get 1-2ml root growth liquid, 1-2ml mineral nutrition liquid, 3-5ml organic nutrient solution for cultivating respectively; Mix and be settled to 1L with clear water; Form a kind of foliage fertilizer that improves acid soil alfalfa growth of seedling; Wherein, contain IAA:4-8 μ mol/L in this foliage fertilizer of every 1L, Zn:1-2mmol/L, Ca:4-8mmol/L, B:1-2mmol/L, succsinic acid: 60-100 μ mol/L, Hydrocerol A: 60-100 μ mol/L, NOVASOY 400: 30-50 μ mol/L.
Adopt the foliage fertilizer of the raising acid soil alfalfa growth of seedling that aforesaid method of the present invention obtains, have the acid soil of promotion clover root growth, strengthen the acidproof effect of coercing of clover.Under the edatope of pH 4.5, utilize this foliage fertilizer to handle the alfalfa of average plant height for 5cm, whenever sprayed 1 time at a distance from 10 days, spray time-division between the lights time, spray altogether 2 times, spray 1-2L/m at every turn 2, to handle 30 days, the comparison of alfalfa root system living weight increases 1.5-1.8 doubly according to (only spraying clear water), and the ground biomass comparison is according to increasing 1.0-1.4 doubly.
Embodiment
Elaborate in the face of embodiments of the invention down: following examples are that prerequisite is implemented with technical scheme of the present invention, provided detailed embodiment and process, but protection scope of the present invention are not limited to following embodiment.
Embodiment one:
1, the preparation of root growth liquid:
(1) the pure article that take by weighing 0.7g draw indole-3-acetic acid (IAA), are dissolved in a small amount of 95% ethanol until dissolving fully;
(2) with the IAA solution of said (1) preparation, be settled to 1 liter (L), form described root growth liquid, contain IAA:4mmol/L in every liter of this root growth liquid with clear water.
2, the preparation of mineral nutrition liquid:
(1) takes by weighing the ZnSO that contains 65g zinc (Zn) respectively 4169g; The MgSO that contains 96g magnesium (Mg) 4512g; The MnCl that contains 55g manganese (Mn) 2126g;
(2) with the ZnSO in said (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described mineral nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this mineral nutrition liquid.
3, the preparation of organic nutrient solution for cultivating:
(1) gets the pure succsinic acid of 2.4g (or non-pure article succsinic acid of suitable content), the pure Hydrocerol A of 3.8g (or non-pure article Hydrocerol A of suitable content) and the pure NOVASOY 400 of 4.4g (or non-pure article NOVASOY 400 of suitable content) respectively;
(2) succsinic acid in said (1), Hydrocerol A and NOVASOY 400 are mixed; Be settled to 1L with clear water; Form described organic nutrient solution for cultivating, contain succsinic acid: 20mmol/L, Hydrocerol A: 20mmol/L and NOVASOY 400 in every liter of this organic nutrient solution for cultivating respectively: 10 μ mol/L.
4, improve the foliage fertilizer preparation of acid soil alfalfa growth of seedling:
Get 1ml root growth liquid, 1ml mineral nutrition liquid, 3ml organic nutrient solution for cultivating respectively; Mix and be settled to 1L with clear water; Form a kind of foliage fertilizer that improves acid soil alfalfa growth of seedling; Wherein, contain IAA:4 μ mol/L, Zn:1mmol/L, Ca:4mmol/L, B:1mmol/L, succsinic acid 60 μ mol/L, Hydrocerol A in per 1 liter of this foliage fertilizer: 60 μ mol/L and NOVASOY 400: 30 μ mol/L.
Under the edatope of pH4.5, contrast and two processing of this growth regulator are set respectively, handle 30 days alfalfa upgrowth situations and see table 1.
It is the alfalfa of 5cm that described control treatment adopts the average plant height of fresh water spraying, whenever sprays 1 time at a distance from 10 days, sprays altogether 2 times, sprays 1L/m at every turn 2
Described foliage fertilizer handled the foliage fertilizer of the raising acid soil alfalfa growth of seedling that adopts the embodiment of the invention two preparations, sprays the alfalfa that average plant height is 5cm, whenever sprays 1 time at a distance from 10 days, sprays altogether 2 times, sprays 1L/m at every turn 2
The alfalfa upgrowth situation of table 1 embodiment one
Figure 2012101969634100002DEST_PATH_IMAGE001
Figure 2012101969634100002DEST_PATH_IMAGE002
Embodiment two:
1, the preparation of root growth liquid:
(1) the pure article that take by weighing 0.7g draw indole-3-acetic acid (IAA), are dissolved in a small amount of 95% ethanol until dissolving fully;
(2) with the IAA solution of said (1) preparation, be settled to 1 liter (L), form described root growth liquid, contain IAA:4mmol/L in every liter of this root growth liquid with clear water.
2, the preparation of mineral nutrition liquid:
(1) takes by weighing the ZnSO that contains 65g zinc (Zn) respectively 4169g; The MgSO that contains 96g magnesium (Mg) 4512g; The MnCl that contains 55g manganese (Mn) 2126g;
(2) with the ZnSO in said (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described mineral nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this mineral nutrition liquid.
3, the preparation of organic nutrient solution for cultivating:
(1) gets the pure succsinic acid of 2.4g (or non-pure article succsinic acid of suitable content), the pure Hydrocerol A of 3.8g (or non-pure article Hydrocerol A of suitable content) and the pure NOVASOY 400 of 4.4g (or non-pure article NOVASOY 400 of suitable content) respectively;
(2) succsinic acid in said (1), Hydrocerol A and NOVASOY 400 are mixed; Be settled to 1L with clear water; Form described organic nutrient solution for cultivating, contain succsinic acid: 20mmol/L, Hydrocerol A: 20mmol/L and NOVASOY 400 in every liter of this organic nutrient solution for cultivating respectively: 10 μ mol/L.
4, improve the foliage fertilizer preparation of acid soil alfalfa growth of seedling:
Get 1.5ml root growth liquid, 1.5ml mineral nutrition liquid, 4ml organic nutrient solution for cultivating respectively; Mix and be settled to 1L with clear water; Form a kind of foliage fertilizer that improves acid soil alfalfa growth of seedling; Wherein, contain IAA:6 μ mol/L, Zn:1.5mmol/L, Ca:6mmol/L, B:1.5mmol/L, succsinic acid 80 μ mol/L, Hydrocerol A in per 1 liter of this foliage fertilizer: 80 μ mol/L and NOVASOY 400: 40 μ mol/L.
Under the edatope of pH4.5, contrast and two processing of this foliage fertilizer are set respectively, the alfalfa upgrowth situation of handling 30 days is seen table 2.
It is the alfalfa of 5cm that described control treatment adopts the average plant height of fresh water spraying, whenever sprays 1 time at a distance from 10 days, sprays altogether 2 times, sprays 1L/m at every turn 2
Described foliage fertilizer handled the foliage fertilizer of the raising acid soil alfalfa growth of seedling that adopts the embodiment of the invention one preparation, sprays the alfalfa that average plant height is 5cm, whenever sprays 1 time at a distance from 10 days, sprays altogether 2 times, sprays 1L/m at every turn 2
The alfalfa upgrowth situation of table 2 embodiment two
Figure 2012101969634100002DEST_PATH_IMAGE003
Embodiment three:
1, the preparation of root growth liquid:
(1) the pure article that take by weighing 0.7g draw indole-3-acetic acid (IAA), are dissolved in a small amount of 95% ethanol until dissolving fully;
(2) with the IAA solution of said (1) preparation, be settled to 1 liter (L), form described root growth liquid, contain IAA:4mmol/L in every liter of this root growth liquid with clear water.
2, the preparation of mineral nutrition liquid:
(1) takes by weighing the ZnSO that contains 65g zinc (Zn) respectively 4169g; The MgSO that contains 96g magnesium (Mg) 4512g; The MnCl that contains 55g manganese (Mn) 2126g;
(2) with the ZnSO in said (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described mineral nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this mineral nutrition liquid.
3, the preparation of organic nutrient solution for cultivating:
(1) gets the pure succsinic acid of 2.4g (or non-pure article succsinic acid of suitable content), the pure Hydrocerol A of 3.8g (or non-pure article Hydrocerol A of suitable content) and the pure NOVASOY 400 of 4.4g (or non-pure article NOVASOY 400 of suitable content) respectively;
(2) succsinic acid in said (1), Hydrocerol A and NOVASOY 400 are mixed; Be settled to 1L with clear water; Form described organic nutrient solution for cultivating, contain succsinic acid: 20mmol/L, Hydrocerol A: 20mmol/L and NOVASOY 400 in every liter of this organic nutrient solution for cultivating respectively: 10 μ mol/L.
4, improve the foliage fertilizer preparation of acid soil alfalfa growth of seedling:
Get 2ml root growth liquid, 2ml mineral nutrition liquid, 5ml organic nutrient solution for cultivating respectively; Mix and be settled to 1L with clear water; Form a kind of foliage fertilizer that improves acid soil alfalfa growth of seedling; Wherein, contain IAA:8 μ mol/L, Zn:2mmol/L, Ca:8mmol/L, B:2mmol/L, succsinic acid in per 1 liter of this growth regulator: 100 μ mol/L, Hydrocerol A: 100 μ mol/L and NOVASOY 400: 50 μ mol/L.
Under the edatope of pH4.5, contrast and two processing of this foliage fertilizer are set respectively, handle 30 days alfalfa upgrowth situations and see table 3.
It is the alfalfa of 5cm that described control treatment adopts the average plant height of fresh water spraying, whenever sprays 1 time at a distance from 10 days, sprays altogether 2 times, sprays 1L/m at every turn 2
Described foliage fertilizer handled the foliage fertilizer of the raising acid soil alfalfa growth of seedling that adopts the embodiment of the invention three preparations, sprays the alfalfa that average plant height is 5cm, whenever sprays 1 time at a distance from 10 days, sprays altogether 2 times, sprays 1L/m at every turn 2
The alfalfa upgrowth situation of table 3 embodiment three
Figure 2012101969634100002DEST_PATH_IMAGE004
Although content of the present invention has been done detailed introduction through the foregoing description, will be appreciated that above-mentioned description should not be considered to limitation of the present invention.After those skilled in the art have read foregoing, for multiple modification of the present invention with to substitute all will be conspicuous.Therefore, protection scope of the present invention should be limited appended claim.

Claims (5)

1. a foliage fertilizer preparation method who promotes acid soil alfalfa growth of seedling is characterized in that said foliage fertilizer is by root growth liquid; Mineral nutrition liquid; Organic nutrient solution for cultivating and clear water mix to be formed, and contains root growth liquid 1-2ml in every liter of this foliage fertilizer, plant nutrition liquid 1-2ml; Organic nutrient solution for cultivating 3-5ml, clear water are surplus; Wherein: said root growth liquid is made up of indole-3-acetic acid IAA; Said mineral nutrition liquid is made up of zinc Zn, calcium Ca, boron; Said organic nutrient solution for cultivating is made up of succsinic acid, Hydrocerol A, NOVASOY 400; This foliage fertilizer prepares through following steps:
1) preparation of root growth liquid:
(1) take by weighing draw indole-3-acetic acid (IAA, be dissolved in ethanol until fully the dissolving;
(2) with the IAA solution of said (1) preparation, be settled to 1 liter, form described root growth liquid with clear water;
2) preparation of mineral nutrition liquid:
(1) takes by weighing the ZnPO that contains zinc Zn respectively 3, calcic Ca CaPO 3, boric acid;
(2) with the ZnPO in said (1) 3, CaPO 3Be dissolved in fully in the clear water,
(3) boric acid is added the ZnPO in said (2) 3And CaPO 3In the mixed solution, be settled to 1 liter, the described mineral nutrition liquid of mineral with clear water;
3) preparation of organic nutrient solution for cultivating:
(1) gets succsinic acid, Hydrocerol A, NOVASOY 400 respectively;
(2) succsinic acid in said (1), Hydrocerol A and NOVASOY 400 are mixed, be settled to 1L, form described organic nutrient solution for cultivating with clear water;
4) foliage fertilizer preparation:
Get root growth liquid, mineral nutrition liquid and organic nutrient solution for cultivating respectively, be settled to 1L, form a kind of foliage fertilizer that improves acid soil alfalfa growth of seedling with its mixing and with clear water.
2. the foliage fertilizer preparation method of promotion acid soil alfalfa growth of seedling according to claim 1 is characterized in that described step 1) is specially:
(1) the pure article that take by weighing 0.7g draw the indole-3-acetic acid IAA or the suitable non-pure article IAA of content, are dissolved in a small amount of 95% ethanol until dissolving fully;
(2) with the IAA solution of said (1) preparation, be settled to 1 liter, form described root growth liquid, contain IAA:4mmol/L in every liter of this root growth liquid with clear water.
3. the foliage fertilizer preparation method of promotion acid soil alfalfa growth of seedling according to claim 1 is characterized in that described step 2), be specially:
(1) takes by weighing the ZnSO that contains 65g zinc Zn respectively 4169g contains the MgSO of 96g magnesium (Mg) 4512g contains the MnCl of 55g manganese (Mn) 2126g;
(2) with the ZnSO in said (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described mineral nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this mineral nutrition liquid.
4. the foliage fertilizer preparation method of promotion acid soil alfalfa growth of seedling according to claim 1 is characterized in that described step 3) is specially:
(1) gets the pure succsinic acid of 2.4g or quite non-pure article succsinic acid, the pure Hydrocerol A of 3.8g or quite non-pure article Hydrocerol A and the pure NOVASOY 400 of 4.4g or the suitable non-pure article NOVASOY 400 of content of content of content respectively;
(2) succsinic acid in said (1) and Hydrocerol A are mixed, be settled to 1L, form described organic nutrient solution for cultivating, contain succsinic acid: 20mmol/L, Hydrocerol A: 20mmol/L and NOVASOY 400 in every liter of this organic nutrient solution for cultivating respectively: 10 μ mol/L with clear water.
5. according to the foliage fertilizer preparation method of each described promotion acid soil alfalfa growth of seedling of claim 1-4, it is characterized in that described step 4) is specially:
Get 1-2ml root growth liquid, 1-2ml mineral nutrition liquid, 3-5ml organic nutrient solution for cultivating respectively; Mix and be settled to 1L with clear water; Form a kind of foliage fertilizer that improves acid soil alfalfa growth of seedling; Wherein, contain IAA:4-8 μ mol/L in this foliage fertilizer of every 1L, Zn:1-2mmol/L, Ca:4-8mmol/L, B:1-2mmol/L, succsinic acid: 60-100 μ mol/L, Hydrocerol A: 60-100 μ mol/L, NOVASOY 400: 30-50 μ mol/L.
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